FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_7153_flu.3520000003ba26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_7153_flu.3520000003ba26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279927
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC121934.355778470815606No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG78312.797515066428033No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT73582.628542441422228No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA60082.1462738499680274No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG57612.058036559531592No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG55311.9758722809875433No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA39241.4017940391602095No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC36561.306054792856709No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC36371.2992673089769833No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32231.151371607597695No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT31261.1167197162117266No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT28471.0170508739778585No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA26590.9498905071679402No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA20030.7155436953205657No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT17900.639452428668903No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15980.5708631178843055No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13860.4951290872263126No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9610.34330378991665683No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7310.2611395113726079No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6850.24470665566379804No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG6260.22362973203728115No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6240.2229152600499416No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT5360.19147849260700112No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT4700.16790091702479573No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT4560.16289961311341886Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3590.12824772172745036No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG3470.12396088980341302No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT3210.1146727539679988No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2890.10324120217056588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT46500.0289.29032
CGGGTTA46950.0286.20341
GGTTATT46900.0285.250523
GTTATTA46850.0284.610474
TTATTAG47000.0283.38835
GGGGGGA51850.0282.198641
TTAGTAG48050.0282.107188
TATTAGT48800.0273.842226
GGGGAGC53600.0273.810643
GGAGCGA40500.0272.055575
GGGAGCG41050.0270.207064
CGAAAGC41150.0267.75829
ATTAGTA50200.0265.911357
TAGTAGA51600.0262.412789
GCGAAAG42200.0261.445538
GAGCGAA42400.0259.51656
AGCGAAA42600.0257.605657
GGGGGAG58600.0252.20992
GGGAGCA15000.0236.04
CAAAAGC17900.0185.405019
Produced by FastQC (version 0.11.2)