FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_7098_flu.3520000003ba69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_7098_flu.3520000003ba69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498865
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT73691.4771531376224027No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT64951.301955438846181No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA61941.2416184739358345No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG58651.175668768103595No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA49200.9862387619897167No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA43610.8741843985847875No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT37920.7601254848506109No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC32400.6494743066761548No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT28120.5636795525843665No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG25270.5065498682008158No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC24210.4853016347107935No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC23450.47006705220851336No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA23190.4648552213524701No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG22490.4508233690477384No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA20480.4105319074298658No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA20200.40491916650797305No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT19930.39950688061900513No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT19500.3908873142032414No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA19280.3864773034788971No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT18520.3712427209766169No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT17700.3548054082767883No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT17400.34879175728904616No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT16590.33255489962214224No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA14700.29466889839936655No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA14470.2900584326420976No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC13950.2796347709300111No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT13600.27261884477764525No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT13530.2712156595471721Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA13480.2702133843825484No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12520.2509697012217734No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG11990.24034558447676227No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT11780.2361360287853427No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA10300.20646868391248135No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT9860.1976486624637928No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC9820.19684684233209382No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA9740.19524320206869594No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8660.1735940585128241No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA8400.1683822276567809No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA7670.15374901025327492No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA7020.14071943311316687No Hit
GGGGGGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5770.1156625539975745No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG5480.10984935804275707No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG5230.10483798221963857No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA4990.10002706142944484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT69050.0289.232451
CGGGTTA69700.0286.746772
TTATTAG75100.0265.73576
GTTATTA77650.0257.958775
GGGTTAT77900.0256.94163
TATTAGT77950.0256.01997
ATTAGTA80300.0252.384838
GGGGGGA55750.0251.874451
GGGGAGC58700.0241.477863
GAGCAGA58100.0239.909646
TTAGTAG85400.0236.79459
GGGAGCA60850.0233.188194
GGAGCAG59750.0232.543935
GGTTATT87200.0230.722464
CAGAAGC61300.0230.032649
GGGGGAG63950.0222.114932
GCAGAAG74400.0190.520848
AGCAGAA96500.0144.901577
CGGGTAA850.0138.823532
CCGGGTA1000.0132.751
Produced by FastQC (version 0.11.2)