##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_7047_flu.3520000003b96a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346323
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.36495121606131	34.0	34.0	34.0	34.0	34.0
2	33.547477354954765	34.0	34.0	34.0	34.0	34.0
3	33.564244938973154	34.0	34.0	34.0	34.0	34.0
4	33.5640572529113	34.0	34.0	34.0	34.0	34.0
5	33.513078831033454	34.0	34.0	34.0	34.0	34.0
6	37.09631471198852	38.0	38.0	38.0	36.0	38.0
7	36.188179820572124	38.0	38.0	38.0	34.0	38.0
8	37.00039558446883	38.0	38.0	38.0	36.0	38.0
9	37.07068545837267	38.0	38.0	38.0	37.0	38.0
10-14	37.08301556639322	38.0	38.0	38.0	37.0	38.0
15-19	37.00651184010302	38.0	38.0	38.0	36.8	38.0
20-24	37.108147018823466	38.0	38.0	38.0	36.8	38.0
25-29	37.03465435446101	38.0	38.0	38.0	36.8	38.0
30-34	36.688828059355	38.0	38.0	38.0	34.6	38.0
35-39	36.97163226236779	38.0	38.0	38.0	36.2	38.0
40-44	36.523219075833836	38.0	38.0	38.0	34.8	38.0
45-49	36.85262774923987	38.0	38.0	38.0	35.8	38.0
50-54	36.829583365817456	38.0	38.0	38.0	35.6	38.0
55-59	36.48801436808991	38.0	37.4	38.0	34.4	38.0
60-64	36.6677604432856	38.0	37.8	38.0	34.8	38.0
65-69	36.55209558706757	38.0	37.6	38.0	34.4	38.0
70-74	36.4203093643795	38.0	37.0	38.0	34.0	38.0
75-79	36.470183614718046	38.0	37.0	38.0	34.0	38.0
80-84	35.81949163064538	38.0	37.0	38.0	31.2	38.0
85-89	36.04342477975762	38.0	37.0	38.0	32.2	38.0
90-94	36.06058159579352	38.0	37.0	38.0	32.2	38.0
95-99	35.16993615786419	38.0	36.4	38.0	28.0	38.0
100-104	35.50981598103505	38.0	36.4	38.0	29.6	38.0
105-109	35.35290003840345	38.0	36.2	38.0	28.8	38.0
110-114	34.92517447585058	38.0	35.6	38.0	26.0	38.0
115-119	34.97655079217956	38.0	35.4	38.0	26.8	38.0
120-124	34.50790678066429	37.8	34.6	38.0	25.4	38.0
125-129	34.08429645157844	37.0	34.0	38.0	23.0	38.0
130-134	33.96970400464306	37.0	34.0	38.0	23.0	38.0
135-139	33.344362921319124	37.0	32.8	38.0	22.4	38.0
140-144	32.07043771277103	36.4	28.8	38.0	17.6	38.0
145-149	31.914771470563608	35.6	28.8	37.6	19.8	38.0
150-154	30.927598224778603	35.0	27.0	37.2	15.0	38.0
155-159	30.77351201046422	35.0	26.6	37.0	15.6	37.6
160-164	30.11129725718477	35.0	24.6	37.0	12.2	37.4
165-169	29.001239882999396	33.8	23.4	36.6	10.0	37.0
170-174	28.38081617449607	32.8	22.6	36.2	9.6	37.0
175-179	27.49275502926459	31.0	20.6	36.2	9.4	37.0
180-184	26.601827773494684	28.8	20.0	35.0	9.0	37.0
185-189	25.838859677237725	28.0	19.8	35.0	9.0	36.8
190-194	24.763365413212522	26.0	17.6	34.8	8.6	36.0
195-199	23.851063891222935	25.0	15.4	33.8	8.0	36.0
200-204	22.96746389930787	24.0	14.8	32.4	8.0	36.0
205-209	22.024252504165187	22.8	12.0	31.2	8.0	35.6
210-214	20.65053259529399	20.4	9.0	28.8	8.0	35.0
215-219	20.07244046742492	20.0	9.0	28.0	8.0	34.0
220-224	19.24091094151991	19.2	9.0	26.6	8.0	33.6
225-229	18.39070867369479	17.2	9.0	25.6	8.0	32.4
230-234	17.44742335911851	15.8	9.0	24.6	7.8	31.4
235-239	16.443750487261887	14.6	8.4	22.8	7.0	29.4
240-244	15.529100290769023	13.4	8.0	21.2	7.0	28.2
245-249	14.814841636275963	12.8	8.0	20.2	7.0	26.6
250-254	14.062645564978359	11.8	8.0	19.0	7.0	25.4
255-259	13.450585147391308	11.0	8.0	17.8	7.0	24.2
260-264	12.854130970221442	10.2	7.8	16.4	7.0	23.0
265-269	12.408205634624327	9.0	7.2	15.4	7.0	21.8
270-274	11.937014867623578	8.6	7.0	14.0	7.0	21.0
275-279	11.483646191561057	8.0	7.0	13.6	7.0	20.2
280-284	11.325977194699746	8.0	7.0	13.0	7.0	19.6
285-289	11.036103290858534	8.0	7.0	13.0	7.0	19.0
290-294	10.808565991863087	8.0	7.0	13.0	7.0	18.8
295-299	10.546198202256274	8.0	7.0	13.0	7.0	17.8
300-301	10.275466832985392	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.028013857562442013
1101	2	-0.01856857061680728
1101	3	0.11732429244716513
1101	4	0.15776249585039182
1101	5	0.23943777923574316
1101	6	0.2224693805043927
1101	7	1.0694718809864057
1101	8	0.31394232309442316
1101	9	0.23305607275625562
1101	10-14	-0.00802131083134583
1101	15-19	0.16846252430479325
1101	20-24	0.20660572705643432
1101	25-29	0.11317248965070092
1101	30-34	0.4309849759023905
1101	35-39	0.2858461589670256
1101	40-44	0.4664057004304283
1101	45-49	0.18956989869651153
1101	50-54	0.1747340810470135
1101	55-59	0.3758430092210361
1101	60-64	0.222741772974274
1101	65-69	0.2469137769945675
1101	70-74	0.20393109380179197
1101	75-79	0.3474492361458843
1101	80-84	0.6867106584852465
1101	85-89	0.3484883576802531
1101	90-94	0.33033592362241393
1101	95-99	1.0317796003261392
1101	100-104	0.37834902816308613
1101	105-109	0.508063371916684
1101	110-114	0.5199534732128441
1101	115-119	0.44959070680589974
1101	120-124	0.8478340803468711
1101	125-129	0.7444786219102539
1101	130-134	0.6086856878883182
1101	135-139	0.44245948628053355
1101	140-144	0.9141994576158012
1101	145-149	1.1434233416687825
1101	150-154	1.0686790216351163
1101	155-159	0.7635726147013564
1101	160-164	1.1340906567101996
1101	165-169	0.4735581915281273
1101	170-174	0.21074682685361168
1101	175-179	0.7399363871197018
1101	180-184	0.633660161174749
1101	185-189	0.4393883851357856
1101	190-194	0.8100367126963697
1101	195-199	0.7657786862312754
1101	200-204	1.0632689580773977
1101	205-209	0.6306932663419538
1101	210-214	0.8368068017800034
1101	215-219	1.092625440663113
1101	220-224	0.7416667033344488
1101	225-229	0.460288014632912
1101	230-234	0.4870637165612379
1101	235-239	0.06720097979019357
1101	240-244	0.10566547589957942
1101	245-249	0.19897634077380566
1101	250-254	0.2768255887763633
1101	255-259	0.09697521011212906
1101	260-264	0.0772681549433738
1101	265-269	0.11531101868807347
1101	270-274	0.11619872383777086
1101	275-279	0.29656217143036834
1101	280-284	0.23946579966682435
1101	285-289	0.08415684938908363
1101	290-294	0.003047301258067847
1101	295-299	0.04926609108011348
1101	300-301	-0.1619264907372706
1102	1	-0.12991365785556752
1102	2	0.03251478806563313
1102	3	0.008270633699439145
1102	4	-0.11429814001340333
1102	5	-0.044353982164437866
1102	6	-0.25325018343487216
1102	7	0.8539351432180027
1102	8	0.16758039075910602
1102	9	0.10761827465377394
1102	10-14	0.09589816197136969
1102	15-19	0.053160601419783404
1102	20-24	0.0240373355684369
1102	25-29	0.027722475884033315
1102	30-34	0.29116684020993233
1102	35-39	0.2029712937781838
1102	40-44	0.44023195665398873
1102	45-49	0.1419641568534189
1102	50-54	0.11302093033144445
1102	55-59	0.12553900029493548
1102	60-64	0.25073295749692903
1102	65-69	0.20510879237836832
1102	70-74	0.0717261062999981
1102	75-79	0.32802440636564256
1102	80-84	0.7026924985057335
1102	85-89	0.28585951308249946
1102	90-94	0.4514063813130491
1102	95-99	0.8739108970742038
1102	100-104	0.6241202561860177
1102	105-109	0.8912788569543011
1102	110-114	0.7027272225200036
1102	115-119	0.5931391889254698
1102	120-124	0.9303023178165049
1102	125-129	0.9724428742599045
1102	130-134	0.9646556729433584
1102	135-139	0.9234906999476493
1102	140-144	1.7192879550530407
1102	145-149	2.0074670872126568
1102	150-154	1.9174399613274922
1102	155-159	1.3304501298236495
1102	160-164	1.7609112535704377
1102	165-169	1.331392387933402
1102	170-174	1.2420845743380475
1102	175-179	1.4575941537452124
1102	180-184	1.2692782004404144
1102	185-189	1.2837403838276522
1102	190-194	1.500534585174936
1102	195-199	1.4032382128261993
1102	200-204	1.634171630734869
1102	205-209	1.4410417133535063
1102	210-214	2.0122533167688417
1102	215-219	1.522573055181958
1102	220-224	1.3690810103299569
1102	225-229	1.3902285354097579
1102	230-234	1.4226967849413015
1102	235-239	0.884538806628342
1102	240-244	1.133151922644215
1102	245-249	0.5615879920646858
1102	250-254	0.7911976302951924
1102	255-259	0.3844398466059431
1102	260-264	0.4446559385577924
1102	265-269	0.44685232049794266
1102	270-274	0.3538732268850726
1102	275-279	0.43892010722358776
1102	280-284	0.6341632876405274
1102	285-289	0.3272514522537531
1102	290-294	-0.1101281822886584
1102	295-299	0.1544376694617533
1102	300-301	0.17505210419683515
1103	1	-0.04550695472438804
1103	2	0.016968196669232327
1103	3	0.12246730554630858
1103	4	0.0956339996825406
1103	5	0.038397125448078384
1103	6	0.05658269220076306
1103	7	1.0656745816294872
1103	8	0.2349067473380657
1103	9	0.1572849457003187
1103	10-14	0.19380644962451754
1103	15-19	0.14842710332323605
1103	20-24	0.12369534639311297
1103	25-29	0.12095352701995665
1103	30-34	0.508957922212069
1103	35-39	0.11837107948248615
1103	40-44	0.5250013140416314
1103	45-49	0.1752980078092108
1103	50-54	0.14264772852199314
1103	55-59	0.26873081166694135
1103	60-64	0.2537354862817196
1103	65-69	0.3764373659741338
1103	70-74	0.24101230095072879
1103	75-79	0.23279158973896585
1103	80-84	0.724221690620503
1103	85-89	0.2470827425449329
1103	90-94	0.4133190838875791
1103	95-99	1.062106954941207
1103	100-104	0.7095855370579116
1103	105-109	0.6631382722877888
1103	110-114	0.7178457003277359
1103	115-119	0.8678798899375693
1103	120-124	1.1026995030652174
1103	125-129	0.894101432171702
1103	130-134	1.0120238613802286
1103	135-139	1.0713466081899057
1103	140-144	1.6580681896793479
1103	145-149	1.4479383944044564
1103	150-154	2.144068292078682
1103	155-159	1.0328524156444203
1103	160-164	1.088548111952516
1103	165-169	1.7435112037884224
1103	170-174	2.1829093380334363
1103	175-179	1.8098504103075754
1103	180-184	1.5144582793021932
1103	185-189	1.4261528946827902
1103	190-194	1.4254116398872299
1103	195-199	1.6572787139962202
1103	200-204	1.8186262338518304
1103	205-209	1.6369428602057852
1103	210-214	1.9673398532892925
1103	215-219	1.5720431093292504
1103	220-224	1.562339654724525
1103	225-229	0.592125900432741
1103	230-234	1.302522529332169
1103	235-239	0.6675528266316633
1103	240-244	0.6537065113075631
1103	245-249	1.2133498395468294
1103	250-254	0.7278318022167234
1103	255-259	0.7992522900905303
1103	260-264	0.6415017065761308
1103	265-269	0.5189244786610665
1103	270-274	0.4854051544354494
1103	275-279	0.3006453906517468
1103	280-284	0.29875683655166974
1103	285-289	0.2061805949156792
1103	290-294	0.07090222882700559
1103	295-299	0.15622776718489284
1103	300-301	-0.2000453979562753
2116	1	0.012566427292654225
2116	2	0.05639590511102455
2116	3	-0.023327040952260347
2116	4	-0.01006077502631797
2116	5	0.15683971572165234
2116	6	0.05962776918949686
2116	7	0.8879180658448078
2116	8	0.09178343872179795
2116	9	0.16675786714507268
2116	10-14	0.05799765747769925
2116	15-19	0.1709789646074924
2116	20-24	0.12625147570981454
2116	25-29	-0.005429010630187747
2116	30-34	0.42517386589280903
2116	35-39	-0.062035603036875386
2116	40-44	0.3275504081400271
2116	45-49	0.16464012109497617
2116	50-54	0.0987402275722431
2116	55-59	0.3031785235794544
2116	60-64	0.25878982161773934
2116	65-69	0.40529254835044526
2116	70-74	0.3677806030711466
2116	75-79	0.38980077773407373
2116	80-84	0.7411404526096916
2116	85-89	0.4348552986273404
2116	90-94	0.48508956692567295
2116	95-99	1.167267522073601
2116	100-104	0.4320501616352104
2116	105-109	0.3620206106067414
2116	110-114	0.5537164023956294
2116	115-119	0.690132988951234
2116	120-124	0.7355839894708254
2116	125-129	0.8865624922384043
2116	130-134	0.9293509005382603
2116	135-139	1.3087725625172766
2116	140-144	1.8711142985001104
2116	145-149	1.7734867896711712
2116	150-154	2.0461483716235485
2116	155-159	1.9715482268754378
2116	160-164	1.688085898110021
2116	165-169	1.7731913827643737
2116	170-174	1.5883698399728097
2116	175-179	2.224913750723026
2116	180-184	2.0988182854931843
2116	185-189	1.3929391600605996
2116	190-194	1.563524700535499
2116	195-199	0.8703208361932759
2116	200-204	0.963045898694034
2116	205-209	1.1596215899267683
2116	210-214	1.8727689312535851
2116	215-219	1.7107923882193745
2116	220-224	1.1889552926077656
2116	225-229	0.6521719576618956
2116	230-234	0.9930664924113373
2116	235-239	0.806391687301506
2116	240-244	0.5602638037803498
2116	245-249	0.863995672587027
2116	250-254	0.786917786654822
2116	255-259	1.6955344117247648
2116	260-264	0.7083130642325557
2116	265-269	0.6542984943167713
2116	270-274	0.45033341865948806
2116	275-279	0.5441779144659602
2116	280-284	0.14243827248662733
2116	285-289	0.0751733392605356
2116	290-294	0.2898818407643642
2116	295-299	0.15591821757956836
2116	300-301	-0.19525734563301178
1117	1	0.0555062275140088
1117	2	0.0630575282775041
1117	3	0.009354139787170368
1117	4	0.11758881378832342
1117	5	0.07697729119903585
1117	6	0.008313657195778035
1117	7	0.87367017781051
1117	8	0.16372686746134235
1117	9	0.07639165299244866
1117	10-14	0.13215672386977673
1117	15-19	0.03618511767772503
1117	20-24	0.1746634736363717
1117	25-29	0.1777929454072975
1117	30-34	0.4342374560243556
1117	35-39	0.22108618348297426
1117	40-44	0.3858658790768601
1117	45-49	0.18501586185906405
1117	50-54	0.21095180240517664
1117	55-59	0.19485674826738375
1117	60-64	0.26655190850603816
1117	65-69	0.31780603270858876
1117	70-74	0.2366278830834716
1117	75-79	0.3537257416590407
1117	80-84	0.7388190415504212
1117	85-89	0.28139489710868304
1117	90-94	0.271640233087993
1117	95-99	0.974807554856362
1117	100-104	0.3676537705397891
1117	105-109	0.45954509259724574
1117	110-114	0.5993754898022843
1117	115-119	0.6530443494354117
1117	120-124	0.7436283722724895
1117	125-129	0.8243453135163676
1117	130-134	0.7059189387567884
1117	135-139	0.9001775976116306
1117	140-144	1.1996198813746233
1117	145-149	1.850987875424682
1117	150-154	2.1620704215261846
1117	155-159	1.6518596630121287
1117	160-164	1.7449576671177134
1117	165-169	1.2745769443441084
1117	170-174	1.1011207891315316
1117	175-179	1.117157968209959
1117	180-184	1.2747544852157873
1117	185-189	1.2050547682927153
1117	190-194	-1.1602778184126485
1117	195-199	1.1845750286028078
1117	200-204	1.5184914779260197
1117	205-209	1.4313555733112366
1117	210-214	1.7861811393259899
1117	215-219	1.6976281522396057
1117	220-224	1.9541103101899715
1117	225-229	1.7158909786624683
1117	230-234	1.2564065504115938
1117	235-239	0.9322221136173745
1117	240-244	0.8075746374989503
1117	245-249	1.4003908308268471
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2108	235-239	-0.9397783004715343
2108	240-244	-0.8923716015268095
2108	245-249	-0.7888099187681821
2108	250-254	-0.7506553272541705
2108	255-259	-0.6539517255476301
2108	260-264	-0.3109543096149672
2108	265-269	-0.3174043575387522
2108	270-274	-0.41793431869222
2108	275-279	-0.0657060946546899
2108	280-284	-0.6953215503877015
2108	285-289	-0.8373262476665317
2108	290-294	-0.6678360139000556
2108	295-299	-0.519763352758492
2108	300-301	2.666863040342337
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	17.0
11	171.0
12	462.0
13	567.0
14	629.0
15	1013.0
16	1392.0
17	1943.0
18	2677.0
19	3112.0
20	4112.0
21	5861.0
22	7939.0
23	11442.0
24	16616.0
25	24724.0
26	35909.0
27	49975.0
28	60429.0
29	57659.0
30	38967.0
31	16559.0
32	3763.0
33	366.0
34	10.0
35	6.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.066218530100514	23.564995683220577	14.034586209983166	33.334199576695745
2	44.667550234896325	23.93488159896975	17.583585265777902	13.813982900356025
3	43.66617290795009	22.777291719002204	18.91413507044002	14.64240030260768
4	43.992457907791284	21.91797830349125	18.912691331502675	15.176872457214795
5	28.229716189799696	22.03174493175446	35.18507289437895	14.553465984066897
6	27.062020137270697	21.477637927599382	35.7481310799456	15.712210855184322
7	16.88452687231284	47.60700271134172	20.128030768964233	15.380439647381202
8	26.55324653574842	20.585984759891776	36.76105831839064	16.099710385969168
9	15.687089797674425	19.371511565792627	37.78524672054701	27.156151915985944
10-14	26.33177698275887	34.10596466304577	23.660628950430667	15.901629403764694
15-19	21.272626998495625	35.25922332620126	21.98710452381159	21.481045151491525
20-24	25.120018017861938	30.73598923548248	26.539790888852316	17.604201857803265
25-29	19.15899319421465	25.534197844209018	37.77762377895779	17.529185182618537
30-34	19.787077381519563	28.44079082244032	30.952030330067597	20.820101465972517
35-39	20.75819394033893	29.955099719048402	27.913537362519957	21.373168978092703
40-44	20.461880960837135	28.70430205328552	29.6800963262619	21.153720659615445
45-49	20.866243362410238	30.85703230798994	26.273103432344953	22.003620897254876
50-54	22.172076356464917	29.517242574128776	27.8243720457492	20.486309023657107
55-59	21.432593272754048	27.02794789834923	29.906070344735987	21.63338848416074
60-64	22.11773402286305	26.020680116538607	28.990855357570823	22.87073050302752
65-69	20.27610063437889	26.893911175405616	29.5913352563936	23.238652933821893
70-74	21.055199914530657	26.07883392093508	29.616283065231013	23.24968309930325
75-79	20.646621795260494	25.855054385645772	30.547321431149534	22.951002387944204
80-84	23.313034363874184	25.762770592770334	27.56582727684849	23.358367766506987
85-89	20.974928029613974	28.32817918532699	26.112155415609127	24.584737369449908
90-94	20.56819789618362	30.29426287021076	28.515576499395074	20.62196273421055
95-99	22.115943786580736	30.240613531298816	26.5515140490236	21.09192863309685
100-104	22.7338640517667	28.667284586931853	26.380344360611335	22.218507000690106
105-109	23.95341920692533	27.346263459256242	23.9015601042957	24.798757229522728
110-114	21.45736783291898	27.932710215607976	29.419241575061434	21.190680376411617
115-119	23.253783317885325	28.11496781905909	26.934047117863958	21.697201745191627
120-124	18.81890605013239	29.02816157171196	27.880446866075886	24.272485512079765
125-129	20.901355093366597	25.372268084995795	30.232124346347195	23.49425247529041
130-134	24.721372822480745	29.023599356669926	25.85372614582341	20.401301675025916
135-139	24.06320111572145	27.52551808571767	24.835428198531428	23.57585260002945
140-144	21.245311457800955	28.94921792661764	26.616135803859404	23.189334811722002
145-149	21.194434097648728	26.923652197515036	28.685302448869987	23.19661125596625
150-154	23.211914888702164	25.63231434239135	25.820924397166806	25.334846371739676
155-159	22.27868203959887	27.138769299180243	29.71255157757354	20.869997083647345
160-164	24.732807234864563	28.767018072724014	26.441039145537548	20.059135546873872
165-169	20.459397729864897	25.266817393011724	28.477346292334033	25.796438584789342
170-174	22.768398287148127	28.42964515784398	26.714079053369254	22.087877501638644
175-179	20.966438844662353	28.810849986861975	28.3348781339963	21.887833034479375
180-184	20.683754760729144	29.090588843362987	26.597886943691297	23.627769452216572
185-189	22.96307204546045	27.185142193847938	27.502822509622522	22.34896325106909
190-194	21.46470202672072	27.637090230796108	26.24648088634021	24.651726856142965
195-199	22.184550260883626	28.869985533735846	27.341123748639273	21.60434045674125
200-204	23.35034057801532	27.29919756989862	27.243815744261862	22.106646107824197
205-209	22.869055765860193	26.88738547540879	27.37121126809366	22.872347490637353
210-214	24.284555169596015	26.944557537327867	26.658292980830034	22.11259431224608
215-219	24.597904268558544	26.750057027688023	27.016628985080406	21.635409718673028
220-224	21.945698091088378	26.65234477640815	27.43207930169235	23.96987783081112
225-229	23.58029931595649	27.615953892753296	26.052269124487832	22.751477666802376
230-234	24.156582150189273	26.065262774923987	27.26350834336732	22.51464673151942
235-239	24.008512284774618	27.079345004518903	24.889424034788334	24.022718675918146
240-244	24.779006880859775	30.083823482702567	23.071352465761734	22.06581717067593
245-249	23.356750778897155	28.743514002823957	23.596642440727297	24.30309277755159
250-254	24.184475186458883	27.171975294739305	24.135041565244006	24.508507953557807
255-259	23.836187605212476	27.49283183617606	25.83900000866243	22.831980549949034
260-264	23.45111355584238	28.43755684722066	25.066715176294963	23.044614420642002
265-269	22.710590980096615	28.46313990119051	25.296789413351124	23.52947970536176
270-274	22.024583986625203	30.052927469443265	23.351899816067657	24.570588727863875
275-279	22.665199827906317	29.39533325825891	23.15722605775533	24.78224085607944
280-284	20.739078836808414	30.080300759695426	25.15357051076596	24.027049892730197
285-289	21.895975722086032	29.797963173107185	24.701160477357842	23.60490062744894
290-294	21.040069530467225	31.21040185029582	23.32429552758552	24.425233091651435
295-299	20.36353346442483	32.416443609000844	21.755124551358126	25.4648983752162
300-301	16.214343257594788	34.22339838820985	28.894846718237023	20.667411635958338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.25
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.0
25	0.0
26	0.25
27	0.25
28	0.0
29	0.25
30	1.25
31	1.75
32	3.75
33	11.25
34	26.25
35	89.25
36	324.0
37	867.5
38	1695.5
39	2747.0
40	4199.25
41	5964.0
42	8530.5
43	11970.75
44	14748.75
45	14779.5
46	12452.0
47	9875.0
48	7915.75
49	6416.25
50	5123.25
51	3664.5
52	2108.75
53	970.75
54	387.5
55	155.5
56	83.25
57	57.75
58	49.25
59	29.25
60	24.75
61	27.0
62	19.5
63	13.0
64	12.75
65	9.5
66	7.25
67	5.25
68	4.75
69	3.5
70	2.0
71	2.0
72	2.25
73	1.0
74	2.25
75	1.75
76	2.25
77	1.5
78	1.5
79	1.5
80	1.25
81	1.25
82	0.75
83	0.25
84	1.25
85	1.0
86	1.0
87	0.25
88	0.25
89	0.0
90	0.0
91	0.25
92	0.0
93	0.5
94	0.25
95	0.25
96	0.0
97	0.5
98	0.5
99	0.5
100	1.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	346323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.605725679806369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.56945927020416	6.4721701356712
2	5.349577286117987	1.4556976211568873
3	4.055264144241671	1.6552443451712073
4	4.5521328538643715	2.4774028347085095
5	4.672306089297122	3.1785057471532765
6	4.292201158655317	3.5039106916326777
7	3.79514851390226	3.614502471599527
8	3.401127234091481	3.701984331933382
9	2.925488702099865	3.582305708412918
>10	19.03271014093903	43.68649190812729
>50	0.25646636754232977	2.2220415360783923
>100	0.0480718553168046	1.1232899384500465
>500	0.015231508092105413	1.4909129855354044
>1k	0.02175929727216163	7.943345177086239
>5k	0.010879648636080815	10.37925304921421
>10k+	0.0021759297272161628	3.5129415180688497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11866	3.4262812461199514	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9177	2.6498384456129105	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7151	2.0648354281985313	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	6710	1.9374976539242268	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	6272	1.8110261230123326	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5749	1.6600110301654813	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4190	1.209853229499629	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3752	1.0833816985877347	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3658	1.0562394065655472	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	3034	0.8760607871842182	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2979	0.8601796588733638	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2801	0.8087825527036898	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1830	0.5284084510702437	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1791	0.5171472873589106	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1660	0.47932132720033027	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1136	0.3280174865660092	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	930	0.26853544234717297	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	854	0.24659061049944705	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	821	0.23706193351293445	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	737	0.21280711936544788	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	617	0.17815738486903845	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT	553	0.15967752647095343	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	524	0.15130384063432115	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	362	0.10452669906416841	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.887477874700785E-4	0.0	0.0
2	2.887477874700785E-4	0.0	0.0
3	2.887477874700785E-4	0.0	0.0
4	2.887477874700785E-4	0.0	0.0
5	2.887477874700785E-4	0.0	0.0
6	2.887477874700785E-4	0.0	0.0
7	2.887477874700785E-4	0.0	0.0
8	2.887477874700785E-4	0.0	0.0
9	2.887477874700785E-4	0.0	0.0
10-14	9.239929199042513E-4	0.0	0.0
15-19	0.001154991149880314	0.0	0.0
20-24	0.001154991149880314	0.0	0.0
25-29	0.001154991149880314	0.0	0.0
30-34	0.0018479858398085026	0.0	0.0
35-39	0.0031184761046768477	0.0	0.0
40-44	0.00415796813956913	0.0	0.0
45-49	0.005717206191907555	0.0	0.0
50-54	0.007738440704198104	0.0	0.0
55-59	0.010799167251380937	0.0	0.0
60-64	0.01570787963837227	0.0	0.0
65-69	0.02408156547500455	0.0	0.0
70-74	0.038634453963496504	0.0	0.0
75-79	0.056536816786641374	0.0	0.0
80-84	0.08246636810145443	0.0	0.0
85-89	0.1276842716192687	0.0	0.0
90-94	0.17988987159385889	0.0	0.0
95-99	0.25288531226629474	0.0	0.0
100-104	0.35181030425354365	0.0	0.0
105-109	0.47054339446124	0.0	0.0
110-114	0.6281996864199028	0.0	0.0
115-119	0.823681938537146	0.0	0.0
120-124	1.0489052127638072	0.0	0.0
125-129	1.3266228347525286	0.0	0.0
130-134	1.6648042434374848	0.0	0.0
135-139	2.011532586631555	0.0	0.0
140-144	2.4167612315670635	0.0	0.0
145-149	2.909422706548511	0.0	0.0
150-154	3.466186190348316	0.0	0.0
155-159	4.148208464352642	0.0	0.0
160-164	4.926268252469516	0.0	0.0
165-169	5.756706889233461	0.0	0.0
170-174	6.692827216211455	0.0	0.0
175-179	7.710951914830953	0.0	0.0
180-184	8.777817239975398	0.0	0.0
185-189	9.908669074823214	0.0	0.0
190-194	11.118406805207854	0.0	0.0
195-199	12.362678770973918	0.0	0.0
200-204	13.574091238525885	0.0	0.0
205-209	14.752470959191276	0.0	0.0
210-214	15.92686596038958	0.0	0.0
215-219	17.076717399652928	0.0	0.0
220-224	18.22679983714625	0.0	0.0
225-229	19.33045163041438	0.0	0.0
230-234	20.284936316675473	0.0	0.0
235-239	21.124499383523474	0.0	0.0
240-244	21.80438492390052	0.0	0.0
245-249	22.35537345195092	0.0	0.0
250-254	22.817312162345555	0.0	0.0
255-259	23.16825622323669	0.0	0.0
260-264	23.465204447870917	0.0	0.0
265-269	23.696087178731993	0.0	0.0
270-274	23.861655160067336	0.0	0.0
275-279	23.97068632461604	0.0	0.0
280-284	24.046973490065632	0.0	0.0
285-289	24.102932811277334	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5220	0.0	292.17432	1
GGGTTAT	5255	0.0	289.3863	2
GTTATTA	5345	0.0	283.96164	4
GGTTATT	5380	0.0	282.38846	3
TTATTAG	5400	0.0	278.33795	5
TTAGTAG	5515	0.0	276.54578	8
TAGTAGA	5555	0.0	274.81998	9
GGGGGGA	3875	0.0	273.3032	1
GGGGAGC	3925	0.0	271.32483	3
TATTAGT	5560	0.0	270.5935	6
GGAGCGA	2925	0.0	269.78632	5
GGGAGCG	2940	0.0	268.40985	4
ATTAGTA	5600	0.0	268.39734	7
CGAAAGC	3115	0.0	256.17175	9
GAGCGAA	3190	0.0	247.3746	6
AGCGAAA	3210	0.0	245.37383	7
GCGAAAG	3265	0.0	244.8545	8
GGGGGAG	4845	0.0	220.10835	2
GGGAGCA	1295	0.0	205.0193	4
CAAAAGC	1745	0.0	136.9341	9
>>END_MODULE