##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6957_flu.3520000003b991.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281933
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.40122653254497	34.0	34.0	34.0	34.0	34.0
2	33.57661572075635	34.0	34.0	34.0	34.0	34.0
3	33.590551655889875	34.0	34.0	34.0	34.0	34.0
4	33.60164294353623	34.0	34.0	34.0	34.0	34.0
5	33.5461403950584	34.0	34.0	34.0	34.0	34.0
6	37.121958053863864	38.0	38.0	38.0	36.0	38.0
7	36.228167685230176	38.0	38.0	38.0	34.0	38.0
8	37.02772644564489	38.0	38.0	38.0	36.0	38.0
9	37.10221932161187	38.0	38.0	38.0	37.0	38.0
10-14	37.13435319739087	38.0	38.0	38.0	37.0	38.0
15-19	37.0476645160375	38.0	38.0	38.0	36.8	38.0
20-24	37.141431474853945	38.0	38.0	38.0	36.8	38.0
25-29	37.07142193357997	38.0	38.0	38.0	36.8	38.0
30-34	36.73075234186846	38.0	38.0	38.0	34.8	38.0
35-39	37.00434429456644	38.0	38.0	38.0	36.4	38.0
40-44	36.55782402201941	38.0	38.0	38.0	34.8	38.0
45-49	36.87995374787627	38.0	38.0	38.0	36.0	38.0
50-54	36.84177801108774	38.0	38.0	38.0	35.6	38.0
55-59	36.521033011389235	38.0	37.4	38.0	34.4	38.0
60-64	36.70286770261019	38.0	37.8	38.0	35.0	38.0
65-69	36.58060603051079	38.0	37.6	38.0	34.6	38.0
70-74	36.44958199288483	38.0	37.0	38.0	34.0	38.0
75-79	36.48807340751172	38.0	37.0	38.0	34.0	38.0
80-84	35.823621924357916	38.0	37.0	38.0	31.2	38.0
85-89	36.060309364281586	38.0	37.0	38.0	32.2	38.0
90-94	36.03863542047224	38.0	37.0	38.0	32.2	38.0
95-99	35.156553507393596	38.0	36.4	38.0	27.8	38.0
100-104	35.521476379139735	38.0	36.4	38.0	29.8	38.0
105-109	35.35015908034888	38.0	36.2	38.0	28.6	38.0
110-114	34.91527064940961	38.0	35.6	38.0	26.0	38.0
115-119	34.940334050997926	38.0	35.2	38.0	26.8	38.0
120-124	34.47876835985855	37.8	34.6	38.0	24.8	38.0
125-129	34.06967541933722	37.0	34.0	38.0	23.0	38.0
130-134	33.888606158200716	37.0	34.0	38.0	23.0	38.0
135-139	33.28731790886487	37.0	32.8	38.0	22.4	38.0
140-144	32.02631972844612	36.4	28.6	38.0	17.6	38.0
145-149	31.925647582936374	35.6	28.6	37.6	19.8	38.0
150-154	30.9007225120862	35.0	27.2	37.2	14.6	38.0
155-159	30.710312024488086	35.0	26.4	37.0	15.4	37.6
160-164	29.96105883312702	35.0	24.6	37.0	11.2	37.4
165-169	28.887742123128543	33.4	23.4	36.6	10.0	37.0
170-174	28.219295364501495	32.6	22.4	36.2	9.6	37.0
175-179	27.345274231821037	30.6	20.4	36.0	9.2	37.0
180-184	26.458098910024724	28.8	20.0	35.0	9.0	37.0
185-189	25.693648490953525	27.8	19.8	35.0	9.0	36.6
190-194	24.60731592257735	25.8	17.0	34.6	8.6	36.0
195-199	23.70410913231158	24.8	15.4	33.8	8.0	36.0
200-204	22.777792596113258	23.6	14.6	32.2	8.0	36.0
205-209	21.86539284156165	22.6	11.0	31.2	8.0	35.4
210-214	20.442729300933202	20.4	9.0	28.4	8.0	35.0
215-219	19.83794305739307	19.6	9.0	27.8	8.0	34.0
220-224	19.04922091418883	18.6	9.0	26.2	8.0	33.4
225-229	18.225235782969712	16.8	9.0	25.4	8.0	32.4
230-234	17.30636144048409	15.6	9.0	24.6	7.6	31.4
235-239	16.25270755817872	14.4	8.0	22.6	7.0	29.2
240-244	15.394673911886866	13.2	8.0	21.2	7.0	28.2
245-249	14.683936254358306	12.6	8.0	20.0	7.0	26.6
250-254	13.964976785264586	11.6	8.0	18.6	7.0	25.2
255-259	13.316461003146136	10.8	8.0	17.2	7.0	24.2
260-264	12.734512100392648	9.8	7.8	16.0	7.0	22.8
265-269	12.297655116641188	9.0	7.0	15.2	7.0	21.8
270-274	11.850892942649494	8.6	7.0	14.0	7.0	21.0
275-279	11.43143725636942	8.0	7.0	13.6	7.0	19.8
280-284	11.230101478010734	8.0	7.0	13.0	7.0	19.4
285-289	10.945404759286783	8.0	7.0	13.0	7.0	19.0
290-294	10.74530473552227	8.0	7.0	13.0	7.0	18.6
295-299	10.483865315518226	8.0	7.0	12.8	7.0	17.8
300-301	10.225074751802733	8.0	7.0	12.5	7.0	15.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.13684816792276422
1101	2	0.029545023302425477
1101	3	0.12160738851665087
1101	4	0.15395576908134245
1101	5	0.21866961214953307
1101	6	0.15669134859459177
1101	7	0.9530570180235074
1101	8	0.28183727067686704
1101	9	0.14211821794852142
1101	10-14	0.10073563540210984
1101	15-19	0.21423337690186628
1101	20-24	0.15540600103486923
1101	25-29	0.21420787755024406
1101	30-34	0.578906425894715
1101	35-39	0.2639804604242073
1101	40-44	0.5420166690045605
1101	45-49	0.290514636963465
1101	50-54	0.2923858891587514
1101	55-59	0.39718626905440146
1101	60-64	0.27554845698055175
1101	65-69	0.3761857874674419
1101	70-74	0.2818185959589101
1101	75-79	0.2794339687346721
1101	80-84	0.9057085047323881
1101	85-89	0.5770131579328819
1101	90-94	0.4746652309403103
1101	95-99	1.0647646068247738
1101	100-104	0.6080895673478892
1101	105-109	0.6571652281002613
1101	110-114	0.8559923478200062
1101	115-119	0.7341139314122529
1101	120-124	0.9837809510204636
1101	125-129	0.976891937612649
1101	130-134	0.82624192723204
1101	135-139	0.8928352506643407
1101	140-144	1.361818541398037
1101	145-149	1.652544639644372
1101	150-154	1.3094030894435562
1101	155-159	0.8971586257652184
1101	160-164	1.2263525581408317
1101	165-169	0.7161245248988664
1101	170-174	0.628573839008375
1101	175-179	1.0203594264879605
1101	180-184	0.6822868277898628
1101	185-189	0.5933150424543285
1101	190-194	1.155118907346253
1101	195-199	1.1745736132574471
1101	200-204	1.1134603430675902
1101	205-209	0.7106477583807198
1101	210-214	1.038382582325987
1101	215-219	0.6610822624714991
1101	220-224	0.46298771708976716
1101	225-229	0.1780446258712729
1101	230-234	0.08971345407232079
1101	235-239	0.04918934950385889
1101	240-244	-0.032162090920030195
1101	245-249	0.1174202539145508
1101	250-254	0.06794149779037895
1101	255-259	0.12203324616381472
1101	260-264	0.19997093001326327
1101	265-269	-0.023626145508288232
1101	270-274	-0.004929502458356438
1101	275-279	-0.04289970947513133
1101	280-284	-0.04136053867181033
1101	285-289	-0.006465662034491615
1101	290-294	-0.1345407267579546
1101	295-299	0.11684010790879285
1101	300-301	-0.11427186819505586
1102	1	-0.09116959418256698
1102	2	0.08431695656393146
1102	3	0.08944622353150322
1102	4	-0.011845647968968365
1102	5	0.14796402557559674
1102	6	0.07207646630118347
1102	7	0.9541998005356689
1102	8	0.30072583305646816
1102	9	0.23029205149408227
1102	10-14	0.1816772881921338
1102	15-19	0.21793599224128002
1102	20-24	0.20270740175972435
1102	25-29	0.18358130622428348
1102	30-34	0.46595706748546917
1102	35-39	0.2335040833712796
1102	40-44	0.5835845666112434
1102	45-49	0.3138274002115793
1102	50-54	0.2078134530729443
1102	55-59	0.39732993314164844
1102	60-64	0.3493591468946846
1102	65-69	0.40661971831276844
1102	70-74	0.2594135285347434
1102	75-79	0.3489967727964469
1102	80-84	0.9211817799470694
1102	85-89	0.38077127985145154
1102	90-94	0.45216874091515535
1102	95-99	1.1563047511418745
1102	100-104	0.8812798896125571
1102	105-109	0.8057400150529546
1102	110-114	0.8873666254182027
1102	115-119	0.795079745889673
1102	120-124	1.0230763439743953
1102	125-129	0.9189104180384149
1102	130-134	0.8833973783046147
1102	135-139	1.1717166298395796
1102	140-144	2.056721731393793
1102	145-149	2.003920876298306
1102	150-154	1.8482478995784106
1102	155-159	1.2871576462373575
1102	160-164	1.7978189113585863
1102	165-169	1.8000145565702645
1102	170-174	1.4496402183468682
1102	175-179	1.5378897102245332
1102	180-184	1.4395500269278827
1102	185-189	1.6155176741192
1102	190-194	1.5795156814344757
1102	195-199	1.9429381282278975
1102	200-204	1.7019802764596967
1102	205-209	1.3855359289491744
1102	210-214	1.544772369115421
1102	215-219	1.9073976051430002
1102	220-224	1.2876195778662058
1102	225-229	1.3181269062121501
1102	230-234	1.2290708837911986
1102	235-239	0.8572900449686465
1102	240-244	0.911315886028417
1102	245-249	0.46686029048359146
1102	250-254	0.4703184854156799
1102	255-259	0.5082251683239996
1102	260-264	0.4445525083591644
1102	265-269	0.44965233426446005
1102	270-274	0.18997368753739963
1102	275-279	0.1741734612565775
1102	280-284	0.1872286146896016
1102	285-289	0.28883260420118084
1102	290-294	0.016492675142329105
1102	295-299	0.04587657899639552
1102	300-301	-0.20728620195285075
1103	1	0.009199083160822852
1103	2	0.08244337950574732
1103	3	0.08604004268070753
1103	4	0.12145003623295736
1103	5	0.08875721463461872
1103	6	-0.05371603015588988
1103	7	0.9028904276778533
1103	8	0.18062332796851166
1103	9	0.1962675369370075
1103	10-14	-0.0790749130389159
1103	15-19	0.21325471344945868
1103	20-24	0.22085890044378687
1103	25-29	0.04374603893320739
1103	30-34	0.4952171910172396
1103	35-39	0.09634758729779946
1103	40-44	0.3530209306996497
1103	45-49	0.08770742734736814
1103	50-54	0.12731108579500727
1103	55-59	0.23386948071274105
1103	60-64	0.16648239807476273
1103	65-69	0.20805358193055667
1103	70-74	0.19257424415238944
1103	75-79	0.3234418920838138
1103	80-84	0.7832811807575979
1103	85-89	0.27307025657647443
1103	90-94	0.3281731025315153
1103	95-99	0.9875393229895622
1103	100-104	0.5474762973171252
1103	105-109	0.7178072720295745
1103	110-114	0.6711856680647159
1103	115-119	0.5523337745243282
1103	120-124	1.05172910887687
1103	125-129	1.0314493546990846
1103	130-134	0.9245539169997556
1103	135-139	0.8518520956765414
1103	140-144	1.3972027168065324
1103	145-149	1.498700078349831
1103	150-154	2.1374386918795167
1103	155-159	0.685988036900369
1103	160-164	0.9700143174541971
1103	165-169	1.5909208706137505
1103	170-174	2.191097239732283
1103	175-179	1.9689545058004043
1103	180-184	1.452759544344154
1103	185-189	1.2580931588517679
1103	190-194	1.446938172173148
1103	195-199	2.0102153501482185
1103	200-204	1.6573305376949463
1103	205-209	1.375584482121468
1103	210-214	1.5359432926201926
1103	215-219	1.7529285466909457
1103	220-224	1.1448789342081298
1103	225-229	0.42964043681272557
1103	230-234	1.596378296052741
1103	235-239	1.6103000219884684
1103	240-244	1.0376518086274853
1103	245-249	1.1730795007753017
1103	250-254	0.7393456815854105
1103	255-259	0.6675474129225929
1103	260-264	0.5414564000717395
1103	265-269	0.40272127124478807
1103	270-274	0.5609046511065383
1103	275-279	0.43168890272816185
1103	280-284	0.5575064013468936
1103	285-289	0.2736738562834269
1103	290-294	0.21970386190688274
1103	295-299	0.07902699437997107
1103	300-301	0.06918717549389974
2116	1	0.12196386126265679
2116	2	0.08984948224491518
2116	3	0.15012726512453156
2116	4	0.16469173005302906
2116	5	0.1250037762102565
2116	6	0.23520831501097916
2116	7	0.9445486763564404
2116	8	0.25822768080222147
2116	9	0.19776637050604506
2116	10-14	0.09935673376129728
2116	15-19	0.2565126388773109
2116	20-24	0.164438177170247
2116	25-29	0.15466006839530877
2116	30-34	0.4706176714037582
2116	35-39	0.002744740204725815
2116	40-44	0.4754780507634777
2116	45-49	0.2794389772759587
2116	50-54	0.2701012301009129
2116	55-59	0.4899293097282964
2116	60-64	0.3915391655328051
2116	65-69	0.5369056101044976
2116	70-74	0.4501715863129476
2116	75-79	0.5198602324191839
2116	80-84	0.8933777588238598
2116	85-89	0.36669520628438335
2116	90-94	0.6726492619259048
2116	95-99	1.3618104978989436
2116	100-104	0.5969091407667193
2116	105-109	0.6513713014053408
2116	110-114	0.826208918979475
2116	115-119	0.7110260595030127
2116	120-124	1.0168946971776833
2116	125-129	0.9496461957519315
2116	130-134	1.0433157561445228
2116	135-139	1.285783062994021
2116	140-144	1.828944489194491
2116	145-149	2.017646681540551
2116	150-154	2.301291592070669
2116	155-159	1.7320830401553202
2116	160-164	1.7293966184400063
2116	165-169	1.6608573428637428
2116	170-174	1.6857596309243945
2116	175-179	2.1443166519643064
2116	180-184	2.0259666855608707
2116	185-189	1.468439254718401
2116	190-194	1.8091288866574367
2116	195-199	0.8939359109330027
2116	200-204	1.017320315235576
2116	205-209	0.9442578244685222
2116	210-214	1.8249163326103854
2116	215-219	1.81438797544196
2116	220-224	1.1765084667550951
2116	225-229	0.5361812567161302
2116	230-234	1.1315302601636184
2116	235-239	0.5813310917032233
2116	240-244	0.7098023412486985
2116	245-249	0.8888972025523199
2116	250-254	0.7686806864289917
2116	255-259	1.3509139865165274
2116	260-264	0.40559024521874854
2116	265-269	0.4349078587564641
2116	270-274	0.5146338629929073
2116	275-279	0.2624143001867054
2116	280-284	0.329919575196044
2116	285-289	0.1370914385681825
2116	290-294	0.0745718764877008
2116	295-299	0.1607945819313663
2116	300-301	0.05879721975093055
1117	1	0.14559450268092178
1117	2	0.09067425874285107
1117	3	0.17079789495706876
1117	4	0.13349681081754738
1117	5	0.0038675980161428924
1117	6	0.11953826799695122
1117	7	0.8889884111563617
1117	8	0.4766450989515434
1117	9	0.18800327739990763
1117	10-14	0.10400268034685922
1117	15-19	0.03307944930586615
1117	20-24	0.13942572604752002
1117	25-29	0.08561055707979648
1117	30-34	0.43160874544997085
1117	35-39	0.24019559800760248
1117	40-44	0.414503578214223
1117	45-49	0.16334824126448666
1117	50-54	0.24749246380406476
1117	55-59	0.33887617434131556
1117	60-64	0.3229527300154942
1117	65-69	0.2924294933006024
1117	70-74	0.31709787213421237
1117	75-79	0.4313459957524799
1117	80-84	0.8621605865179731
1117	85-89	0.3687280066037033
1117	90-94	0.3560470585187119
1117	95-99	1.034195143986821
1117	100-104	0.4529829927025659
1117	105-109	0.3860970411433087
1117	110-114	0.5848803753546221
1117	115-119	0.7781402601409297
1117	120-124	0.8848831255810765
1117	125-129	0.9283976925358246
1117	130-134	0.7226048588512697
1117	135-139	0.9834242375322759
1117	140-144	1.2376062319278134
1117	145-149	1.7020405679509913
1117	150-154	2.2230264838253717
1117	155-159	1.5866461558677578
1117	160-164	2.223335305932725
1117	165-169	1.3021106499172568
1117	170-174	1.2392302268474928
1117	175-179	1.40551542133419
1117	180-184	1.5285275547728645
1117	185-189	1.2439831303555309
1117	190-194	-0.8315649146261741
1117	195-199	1.0934078420403175
1117	200-204	1.357983386088648
1117	205-209	1.572766833429803
1117	210-214	1.8491534585027196
1117	215-219	2.054406766923627
1117	220-224	2.165159047630876
1117	225-229	1.4671391630907529
1117	230-234	1.5904351971662365
1117	235-239	1.4130526669380288
1117	240-244	0.8993670641273273
1117	245-249	1.0135606690155292
1117	250-254	0.9038596117116597
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2108	230-234	-0.7416608235258728
2108	235-239	-1.1616801447332517
2108	240-244	-0.8512857399878424
2108	245-249	-0.935849736616678
2108	250-254	-0.88415305929977
2108	255-259	-0.679037704304724
2108	260-264	-0.5240111772776892
2108	265-269	-0.20075416980058058
2108	270-274	-0.4934951471509468
2108	275-279	-0.39872626774071307
2108	280-284	-0.5645329029255723
2108	285-289	-0.8583218673435322
2108	290-294	-0.49353442512867574
2108	295-299	-0.4557494372637656
2108	300-301	2.6890552614617835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	49.0
12	190.0
13	340.0
14	464.0
15	731.0
16	1071.0
17	1730.0
18	2313.0
19	2640.0
20	3531.0
21	4792.0
22	6906.0
23	9970.0
24	14316.0
25	20951.0
26	30323.0
27	40964.0
28	48801.0
29	45500.0
30	30166.0
31	12893.0
32	2970.0
33	302.0
34	15.0
35	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.604313790865206	23.28212731393629	14.860622913954735	33.252935981243766
2	43.50891878566894	24.704451057520757	17.490325715684225	14.296304441126084
3	42.73426665200598	23.474726264750846	19.125820673706166	14.665186409537018
4	43.07264491918293	22.556777674128252	19.135397417116835	15.23517998957199
5	27.68246356403826	22.79264931739101	34.828487619398935	14.69639949917179
6	26.587877261618896	22.01054860551975	35.374007299606646	16.02756683325471
7	17.192382587352313	46.645479599763064	20.707756807468442	15.454381005416181
8	25.976029765937298	21.162120078174603	36.47072176722838	16.391128388659716
9	15.96407657138398	19.679853014723356	37.72669393082754	26.629376483065126
10-14	26.121738143459616	33.89067615355421	23.755644071463788	16.231941631522385
15-19	21.21681392387553	35.02101563137341	22.248690291665042	21.513480153086018
20-24	25.23684705231385	30.641251644894353	26.521833201505324	17.600068101286475
25-29	19.534286514881195	25.981633934303538	37.04745453707086	17.4366250137444
30-34	19.7656180723789	28.094547286057324	31.349646901923506	20.79018773964027
35-39	20.808773715740976	30.03515019525916	27.602018919388648	21.55405716961122
40-44	20.644053729077473	28.766338101605697	29.178918395505317	21.41068977381151
45-49	21.185671773080838	30.369626826231766	26.45862669499491	21.986074705692488
50-54	22.114544945075604	29.56078217165071	27.8298035348824	20.494869348391283
55-59	21.733532435011156	26.86872412949176	29.626329659883734	21.771413775613354
60-64	22.17682924666499	26.116985241174323	28.20492812122029	23.5012573909404
65-69	20.545874374408104	27.153047000528495	29.16473062748951	23.136347997573893
70-74	20.805723345617576	26.404358482334455	29.32547803910858	23.464440132939387
75-79	20.60603051079512	26.2408444559523	30.223209060308655	22.929915972943927
80-84	23.69357258639464	25.32828721717571	27.68125760375692	23.296882592672727
85-89	21.039112129477573	28.2421000734217	26.153944376855492	24.564843420245236
90-94	20.60666896035583	30.17411938297397	28.536709076269894	20.682502580400307
95-99	22.06013485473499	29.94895950456314	26.376692334703634	21.614213305998234
100-104	22.70532360525373	28.689582276640195	26.293055442250463	22.31203867585561
105-109	23.367041105510882	27.767022661412465	24.189222262026792	24.67671397104986
110-114	21.630529239216408	28.33800938520854	28.78960604115162	21.241855334423427
115-119	23.264747298116927	27.94280910712829	26.76671407745812	22.025729517296664
120-124	19.30380622346444	28.767260306526726	27.721621803761888	24.207311666246945
125-129	21.0281875481054	25.197688812590226	30.44475105787545	23.32937258142892
130-134	24.318685645171016	29.276601178294133	25.8769282063469	20.527784970187952
135-139	24.25498256678005	27.67317057598791	24.816747241365857	23.255099615866182
140-144	21.380327950257687	29.207932381097635	26.553613801860728	22.85812586678395
145-149	20.976189378327476	27.22923531477337	28.72483888015947	23.069736426739688
150-154	23.30965158388695	25.618214256578693	25.955883135354853	25.116251024179505
155-159	22.274228274093492	26.996910613514558	29.465298492904342	21.263562619487608
160-164	24.896908130655156	28.771374759251312	26.160541688982843	20.17117542111069
165-169	20.804375507656076	25.442498749702942	28.25671347447798	25.496412268163006
170-174	22.64864347203059	28.406749121245117	26.387049405355175	22.55755800136912
175-179	21.49141817382144	28.258274128959716	28.25373404319466	21.996573654024182
180-184	20.66554819762142	28.96106521762263	26.530345862314803	23.84304072244115
185-189	22.891112427420698	27.152692306328095	27.524979339062828	22.431215927188376
190-194	21.57753792567738	27.315922577349937	26.67066288799112	24.435876608981566
195-199	22.22329418691675	29.10379416386163	26.92476581315419	21.748145836067433
200-204	23.493028485491234	26.976196472211484	27.24966570071613	22.281109341581157
205-209	22.703692011931913	26.857302976239033	28.0090659837621	22.429939028066954
210-214	24.611804932377552	26.47409136213214	27.038622651481028	21.875481054009285
215-219	24.457229199845354	26.328099229249503	27.051888214575804	22.162783356329342
220-224	21.805677235371522	26.48643472030589	27.339474272256176	24.368413772066415
225-229	23.470186178985788	27.40984560161457	25.821950605285654	23.29801761411399
230-234	23.491396892169416	26.057538493188098	27.55165234293254	22.899412271709945
235-239	23.491325953329337	26.567659692196372	25.246636612244757	24.694377742229538
240-244	24.58569943922847	29.51424629255887	23.163517573324157	22.7365366948885
245-249	23.194163152238296	28.26260139820454	23.82473850170076	24.718496947856405
250-254	23.70591594456839	26.57446982084396	24.48801665643965	25.231597578148
255-259	23.561200710807178	26.955056697867935	25.986103081228517	23.49763951009637
260-264	23.34625602536773	27.838599951052203	25.12533119570962	23.68981282787045
265-269	22.57869777571267	27.602373613589045	25.29707412754094	24.521854483157345
270-274	21.954294105337084	28.938364788797337	23.817573678852778	25.289767427012798
275-279	22.4845619349278	28.599844643940227	22.894659369424648	26.02093405170732
280-284	20.891630280953276	28.924247959621614	25.2201054860552	24.964016273369914
285-289	21.657131304246043	28.619069069601643	24.849733802002604	24.87406582414971
290-294	21.022796196259392	29.921648051132717	23.64625638006193	25.409299372545956
295-299	20.24019891250758	31.159814565872036	21.91102141288888	26.688965108731505
300-301	15.941908183859285	32.096100846655055	29.78881507308474	22.173175896400917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.5
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	1.25
31	0.75
32	2.25
33	5.75
34	14.75
35	55.25
36	192.0
37	589.75
38	1390.5
39	2361.5
40	3296.75
41	4409.0
42	6296.75
43	8971.0
44	11261.0
45	11612.5
46	10280.5
47	8605.75
48	7156.25
49	5814.75
50	4664.75
51	3363.75
52	2001.75
53	934.25
54	359.5
55	143.5
56	71.75
57	41.0
58	28.5
59	19.75
60	14.25
61	14.0
62	9.0
63	4.75
64	6.0
65	7.75
66	5.0
67	3.5
68	4.0
69	3.5
70	1.5
71	2.0
72	1.0
73	1.5
74	1.0
75	1.25
76	1.0
77	1.25
78	0.25
79	0.75
80	0.5
81	1.0
82	0.25
83	0.75
84	0.0
85	0.75
86	0.75
87	0.75
88	0.75
89	0.5
90	0.0
91	0.5
92	0.75
93	0.5
94	0.5
95	0.25
96	0.0
97	0.0
98	0.0
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	281933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.558953811708733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.8372391156835	6.236653857063587
2	6.430918002628254	1.8725487633430187
3	5.636145969148878	2.4616916652266063
4	5.604147828161985	3.2636211753679265
5	5.703972261486256	4.15219343491231
6	5.197289464372046	4.540025835472383
7	4.306732841328712	4.3891067161391675
8	3.918765168675431	4.564249687174287
9	3.323033498198164	4.3541902093525175
>10	16.77138263455847	39.54963871625204
>50	0.17990458293255496	1.6191183010022994
>100	0.037723329584373196	1.2115380898331192
>500	0.015070086640401716	1.4243021472226396
>1k	0.027628492174015286	9.924040044522519
>5k	0.01004672442691465	10.437081357115654
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9604	3.4064831005948224	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7668	2.7197951286298516	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5721	2.029205520460535	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5549	1.9681981179925727	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4118	1.4606307172271427	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3995	1.4170033305785417	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3170	1.12438061525256	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3142	1.1144491776414962	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2964	1.0513136099711633	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1943	0.6891708313677364	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1933	0.685623889363785	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1546	0.54835723381087	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1494	0.5299131353903233	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1466	0.5199816977792596	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1368	0.4852216661405369	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	864	0.3064557891413917	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	724	0.2567986010860736	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG	655	0.23232470125880972	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	605	0.21458999123905326	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA	542	0.1922442566141601	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	505	0.1791205711995403	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	500	0.17734710019756467	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	485	0.17202668719163772	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	429	0.15216381196951048	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	3.546942003951293E-4	0.0	0.0
8	3.546942003951293E-4	0.0	0.0
9	3.546942003951293E-4	0.0	0.0
10-14	0.0011350214412644138	0.0	0.0
15-19	0.002128165202370776	0.0	0.0
20-24	0.0028375536031610346	0.0	0.0
25-29	0.003688819684109345	0.0	0.0
30-34	0.006739189807507458	0.0	0.0
35-39	0.008157966609087975	0.0	0.0
40-44	0.00893829384995726	0.0	0.0
45-49	0.010073315291221675	0.0	0.0
50-54	0.014045890335647121	0.0	0.0
55-59	0.021494468543944836	0.0	0.0
60-64	0.031638722675245534	0.0	0.0
65-69	0.04625212373152487	0.0	0.0
70-74	0.06590218243341503	0.0	0.0
75-79	0.09200767558249655	0.0	0.0
80-84	0.13308126398825254	0.0	0.0
85-89	0.18635633288760098	0.0	0.0
90-94	0.25573451848488826	0.0	0.0
95-99	0.3480259494277009	0.0	0.0
100-104	0.46791258916125467	0.0	0.0
105-109	0.6134081501633366	0.0	0.0
110-114	0.7911808834013755	0.0	0.0
115-119	1.0059127523205869	0.0	0.0
120-124	1.2554046528785208	0.0	0.0
125-129	1.560654481738569	0.0	0.0
130-134	1.9392550712403303	0.0	0.0
135-139	2.3278580371932343	0.0	0.0
140-144	2.7605140228352125	0.0	0.0
145-149	3.266520769118904	0.0	0.0
150-154	3.840983496078856	0.0	0.0
155-159	4.515824681750629	0.0	0.0
160-164	5.270755817871622	0.0	0.0
165-169	6.094213873508954	0.0	0.0
170-174	7.003011353761354	0.0	0.0
175-179	7.963736064951601	0.0	0.0
180-184	8.977168334320565	0.0	0.0
185-189	10.081757013191076	0.0	0.0
190-194	11.217771598216597	0.0	0.0
195-199	12.354921204683382	0.0	0.0
200-204	13.47362671272962	0.0	0.0
205-209	14.563389174023616	0.0	0.0
210-214	15.63782884586054	0.0	0.0
215-219	16.712552273057785	0.0	0.0
220-224	17.750884075294486	0.0	0.0
225-229	18.68990150142055	0.0	0.0
230-234	19.541593215409335	0.0	0.0
235-239	20.285741647838314	0.0	0.0
240-244	20.930291948796345	0.0	0.0
245-249	21.39749514955681	0.0	0.0
250-254	21.832633994601554	0.0	0.0
255-259	22.163918377770607	0.0	0.0
260-264	22.437387606275248	0.0	0.0
265-269	22.6567304997996	0.0	0.0
270-274	22.7994594460386	0.0	0.0
275-279	22.896574718106784	0.0	0.0
280-284	22.964888821102885	0.0	0.0
285-289	23.007664941670537	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4230	0.0	291.513	1
GGGTTAT	4255	0.0	289.80026	2
GTTATTA	4270	0.0	288.09134	4
GGTTATT	4315	0.0	285.42874	3
TTAGTAG	4465	0.0	276.50055	8
TTATTAG	4440	0.0	276.3964	5
GGGGGGA	3025	0.0	275.98346	1
GGAGCGA	2280	0.0	273.65134	5
TATTAGT	4480	0.0	273.59933	6
GGGGAGC	3080	0.0	272.49185	3
TAGTAGA	4550	0.0	269.71426	9
ATTAGTA	4590	0.0	267.04248	7
GGGAGCG	2365	0.0	263.19238	4
CGAAAGC	2460	0.0	256.626	9
GAGCGAA	2555	0.0	244.77495	6
GCGAAAG	2645	0.0	239.23439	8
AGCGAAA	2635	0.0	236.78369	7
GGGGGAG	3755	0.0	223.90146	2
GGGAGCA	1025	0.0	208.65852	4
GCAAAAG	1465	0.0	130.88737	8
>>END_MODULE