FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6957_flu.3520000003b991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6957_flu.3520000003b991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281933
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC96043.4064831005948224No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT76682.7197951286298516No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA57212.029205520460535No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG55491.9681981179925727No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG41181.4606307172271427No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA39951.4170033305785417No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT31701.12438061525256No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT31421.1144491776414962No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA29641.0513136099711633No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA19430.6891708313677364No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT19330.685623889363785No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA15460.54835723381087No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14940.5299131353903233No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14660.5199816977792596No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG13680.4852216661405369No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8640.3064557891413917No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT7240.2567986010860736Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG6550.23232470125880972No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6050.21458999123905326No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA5420.1922442566141601No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5050.1791205711995403No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5000.17734710019756467No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4850.17202668719163772No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4290.15216381196951048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA42300.0291.5131
GGGTTAT42550.0289.800262
GTTATTA42700.0288.091344
GGTTATT43150.0285.428743
TTAGTAG44650.0276.500558
TTATTAG44400.0276.39645
GGGGGGA30250.0275.983461
GGAGCGA22800.0273.651345
TATTAGT44800.0273.599336
GGGGAGC30800.0272.491853
TAGTAGA45500.0269.714269
ATTAGTA45900.0267.042487
GGGAGCG23650.0263.192384
CGAAAGC24600.0256.6269
GAGCGAA25550.0244.774956
GCGAAAG26450.0239.234398
AGCGAAA26350.0236.783697
GGGGGAG37550.0223.901462
GGGAGCA10250.0208.658524
GCAAAAG14650.0130.887378
Produced by FastQC (version 0.11.2)