##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6955_flu.3520000003bb4f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406625
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18183584383646	34.0	34.0	34.0	34.0	34.0
2	33.389651398708885	34.0	34.0	34.0	34.0	34.0
3	33.41754687980326	34.0	34.0	34.0	34.0	34.0
4	33.41399077774362	34.0	34.0	34.0	34.0	34.0
5	33.36322410083	34.0	34.0	34.0	34.0	34.0
6	36.864120504150016	38.0	38.0	38.0	36.0	38.0
7	36.11988687365509	38.0	38.0	38.0	34.0	38.0
8	36.792103289271445	38.0	38.0	38.0	35.0	38.0
9	36.831650783891796	38.0	38.0	38.0	36.0	38.0
10-14	36.847323701198896	38.0	38.0	38.0	36.0	38.0
15-19	36.728291669228405	38.0	38.0	38.0	35.6	38.0
20-24	36.80719778665847	38.0	38.0	38.0	35.8	38.0
25-29	36.735989179219175	38.0	38.0	38.0	35.6	38.0
30-34	36.377099784814014	38.0	37.4	38.0	33.4	38.0
35-39	36.648004426683066	38.0	38.0	38.0	34.8	38.0
40-44	36.13587506916692	38.0	37.2	38.0	33.0	38.0
45-49	36.44184051644636	38.0	37.2	38.0	34.0	38.0
50-54	36.384265109130034	38.0	37.2	38.0	34.0	38.0
55-59	36.016178051029826	38.0	37.0	38.0	32.8	38.0
60-64	36.155984998462955	38.0	37.0	38.0	33.2	38.0
65-69	36.07657571472487	38.0	37.0	38.0	32.4	38.0
70-74	35.955236889025514	38.0	37.0	38.0	32.0	38.0
75-79	35.95256268060252	38.0	37.0	38.0	32.0	38.0
80-84	35.247964094681834	38.0	36.6	38.0	28.4	38.0
85-89	35.435474946203506	38.0	37.0	38.0	29.4	38.0
90-94	35.38166025207501	38.0	36.8	38.0	28.8	38.0
95-99	34.448604980018445	38.0	35.0	38.0	23.8	38.0
100-104	34.807719151552405	38.0	36.0	38.0	25.2	38.0
105-109	34.53741063633569	38.0	35.0	38.0	24.0	38.0
110-114	34.056696464801725	37.2	34.2	38.0	23.4	38.0
115-119	34.008147064248384	37.0	34.0	38.0	23.0	38.0
120-124	33.54985650169075	37.0	33.2	38.0	22.0	38.0
125-129	33.181599262219486	37.0	32.0	38.0	21.8	38.0
130-134	32.96669019366739	37.0	31.4	38.0	21.4	38.0
135-139	32.34247082692899	37.0	30.0	38.0	18.8	38.0
140-144	30.9577960036889	35.6	26.2	37.8	14.0	38.0
145-149	30.86243098678143	35.0	26.8	37.6	14.8	38.0
150-154	29.898060375038426	35.0	24.0	37.0	10.2	38.0
155-159	29.442112511527817	34.4	23.8	37.0	10.0	37.0
160-164	28.839202705195202	33.4	22.8	37.0	10.2	37.4
165-169	27.60288078696588	31.2	20.6	36.6	9.6	37.0
170-174	27.10414755610206	29.8	20.2	35.8	9.2	37.0
175-179	26.42825305871503	28.8	19.8	35.4	9.0	37.0
180-184	25.27089873962496	27.0	17.4	35.0	8.8	36.8
185-189	24.732721303412234	26.0	16.2	35.0	8.4	36.2
190-194	23.81514761758377	24.8	15.2	34.4	8.0	36.0
195-199	23.017575407316322	24.0	15.0	33.6	8.0	36.0
200-204	22.107446910544112	23.0	12.0	31.4	8.0	35.6
205-209	21.293796003688904	21.8	9.0	30.0	8.0	35.0
210-214	19.996960836151242	20.2	9.0	28.2	8.0	34.8
215-219	19.569905932984938	19.6	9.0	27.4	8.0	34.0
220-224	18.91563971718414	18.2	9.0	26.0	8.0	33.6
225-229	18.130068244697203	16.8	9.0	25.2	8.0	32.4
230-234	17.36706990470335	15.8	9.0	24.8	8.0	31.2
235-239	16.54462219489702	14.8	9.0	22.8	8.0	29.6
240-244	15.577175530279742	13.6	8.0	21.6	7.0	28.4
245-249	14.913184875499539	12.8	8.0	20.2	7.0	26.8
250-254	14.382381309560406	12.2	8.0	19.6	7.0	26.0
255-259	13.680898616661542	11.4	8.0	18.4	7.0	24.6
260-264	13.202502551490932	10.6	8.0	17.2	7.0	23.8
265-269	12.731996802951121	9.8	7.8	16.0	7.0	22.4
270-274	12.20501592376268	9.0	7.0	15.0	7.0	21.6
275-279	11.854929726406393	8.6	7.0	14.2	7.0	21.0
280-284	11.688216907470029	8.2	7.0	14.0	7.0	20.6
285-289	11.323063264678757	8.0	7.0	13.0	7.0	19.4
290-294	11.000584322164157	8.0	7.0	13.0	7.0	19.0
295-299	10.78215210574854	8.0	7.0	13.0	7.0	18.8
300-301	10.523879495849984	8.0	7.0	13.0	7.0	16.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.17583460789342098
1101	2	0.10108184987340252
1101	3	0.13736853385359638
1101	4	0.12431946631537016
1101	5	0.16408272102304267
1101	6	0.2029309642989645
1101	7	0.9246165090860217
1101	8	0.47450227875574313
1101	9	0.27292759674111977
1101	10-14	0.1718090803264758
1101	15-19	0.2696232338097033
1101	20-24	0.23530502729596492
1101	25-29	0.119021206748684
1101	30-34	0.5690243906778534
1101	35-39	0.28984824578478197
1101	40-44	0.506339756475775
1101	45-49	0.3262949840712537
1101	50-54	0.3143901218766061
1101	55-59	0.5051986788157876
1101	60-64	0.34571079001148775
1101	65-69	0.31112414207631645
1101	70-74	0.2552489157704585
1101	75-79	0.3585987329751319
1101	80-84	0.8128906603795443
1101	85-89	0.49477857314243323
1101	90-94	0.5015199037932163
1101	95-99	1.084809091465381
1101	100-104	0.5904435674048898
1101	105-109	0.7022955106205018
1101	110-114	0.4851655418405798
1101	115-119	0.635718248055376
1101	120-124	0.9284641217845078
1101	125-129	0.6231931134730431
1101	130-134	0.7129214954029521
1101	135-139	0.6427969919647722
1101	140-144	1.1900923323268486
1101	145-149	1.4136764365054546
1101	150-154	0.9254194026136062
1101	155-159	0.6903153285742896
1101	160-164	1.1942457899751773
1101	165-169	0.4991699898677524
1101	170-174	0.42501001998025956
1101	175-179	0.6622057405708972
1101	180-184	0.29208824578777026
1101	185-189	0.277985941748355
1101	190-194	0.6476112764719986
1101	195-199	1.1121211117032814
1101	200-204	0.975016449515838
1101	205-209	0.552749966037684
1101	210-214	0.7995214105870332
1101	215-219	1.0363888911529564
1101	220-224	0.6334024445023019
1101	225-229	0.3873837728344789
1101	230-234	0.32266578537432267
1101	235-239	0.2672497274095953
1101	240-244	0.2035682756189363
1101	245-249	0.07839441086881216
1101	250-254	-0.036496269675525994
1101	255-259	-0.05577191090253564
1101	260-264	0.0676413795371289
1101	265-269	0.053753867799931854
1101	270-274	-0.06319941294318454
1101	275-279	0.16366190016790583
1101	280-284	-0.10613790289189495
1101	285-289	-0.01587720623659372
1101	290-294	-0.09344548011815945
1101	295-299	-0.10392612253297351
1101	300-301	-0.26635620197357923
1102	1	-0.13269806616778368
1102	2	-9.731590239567822E-4
1102	3	0.10434455669836495
1102	4	0.055444929646291996
1102	5	0.11393484197087389
1102	6	0.005980156503049727
1102	7	0.9784033381793336
1102	8	0.18535916840738054
1102	9	0.15030843189729381
1102	10-14	0.17401917759958963
1102	15-19	0.20624396008750523
1102	20-24	0.15636750575591662
1102	25-29	0.19696489665632555
1102	30-34	0.49074696183915023
1102	35-39	0.2080073010468908
1102	40-44	0.4747908772618388
1102	45-49	0.1476417912200887
1102	50-54	0.15267848778670157
1102	55-59	0.18677903288149622
1102	60-64	0.18057349635639497
1102	65-69	0.20416397974345557
1102	70-74	0.2013094600216334
1102	75-79	0.3379577361894377
1102	80-84	0.6369826372815837
1102	85-89	0.38581896645499825
1102	90-94	0.4201196069800801
1102	95-99	0.8413375735686728
1102	100-104	0.6218792074873178
1102	105-109	0.781812239767973
1102	110-114	0.8760065358128841
1102	115-119	0.777299340637164
1102	120-124	1.0976847059858983
1102	125-129	1.04509064260926
1102	130-134	0.903473061408711
1102	135-139	1.0899649586541784
1102	140-144	1.8958075675618105
1102	145-149	1.680239066185358
1102	150-154	1.7074571091343564
1102	155-159	1.2131149023450156
1102	160-164	1.5412164673702513
1102	165-169	1.3597353415069051
1102	170-174	1.4938040232297354
1102	175-179	1.2967345321475996
1102	180-184	1.4045661782241048
1102	185-189	1.402367522454096
1102	190-194	1.925026048200202
1102	195-199	1.3663649415773058
1102	200-204	1.790941317813811
1102	205-209	0.9482808887607668
1102	210-214	1.2691657623457537
1102	215-219	1.2776163752045306
1102	220-224	1.0587910446274975
1102	225-229	1.125539205851073
1102	230-234	0.8899892624486725
1102	235-239	0.8803638386934161
1102	240-244	1.144390103340717
1102	245-249	0.39265445292905987
1102	250-254	0.9312868457369525
1102	255-259	0.6197209606589666
1102	260-264	0.22669576245419698
1102	265-269	0.4114059493602422
1102	270-274	0.26814901190753027
1102	275-279	0.3807724384406228
1102	280-284	0.3224377457713903
1102	285-289	0.255185448060514
1102	290-294	-0.01767126909950001
1102	295-299	0.05509133458034299
1102	300-301	-0.011429684323045919
1103	1	-0.09862355482054141
1103	2	0.03161088372754506
1103	3	0.12593606991666917
1103	4	0.20249212753081025
1103	5	0.0046973888338968095
1103	6	0.12508214701652065
1103	7	1.017438598506459
1103	8	0.28863733627884613
1103	9	0.1295549296844669
1103	10-14	0.0612432639863556
1103	15-19	0.10189337977998747
1103	20-24	0.16309023544515355
1103	25-29	0.10310904490573591
1103	30-34	0.42866510430274474
1103	35-39	0.16396675892022472
1103	40-44	0.42633243777468977
1103	45-49	-0.038858377935582666
1103	50-54	0.018930723058076637
1103	55-59	0.26074873675550236
1103	60-64	0.23113257311165114
1103	65-69	0.3937893862348858
1103	70-74	-0.001164010155179085
1103	75-79	0.337889901312991
1103	80-84	0.7705256891260674
1103	85-89	0.3332837026757076
1103	90-94	0.21542225754588884
1103	95-99	1.0097078794987837
1103	100-104	0.4411413780652751
1103	105-109	0.6267921136952168
1103	110-114	0.5084719969864793
1103	115-119	0.6368485235117021
1103	120-124	0.984659891884526
1103	125-129	0.9396339913391998
1103	130-134	0.980334291619215
1103	135-139	0.9721387125982659
1103	140-144	1.3275358207015415
1103	145-149	1.5089100894736092
1103	150-154	1.9475016074324394
1103	155-159	0.7341766821388518
1103	160-164	1.1482336660142458
1103	165-169	1.6953443364460448
1103	170-174	1.8567939705498233
1103	175-179	1.322302584509906
1103	180-184	1.2001634703524964
1103	185-189	1.2950952094401558
1103	190-194	1.0223796249796138
1103	195-199	1.405911984114784
1103	200-204	1.4161769274991762
1103	205-209	1.1882140550097269
1103	210-214	1.8555302617485303
1103	215-219	1.9604805604610576
1103	220-224	1.7121408447476298
1103	225-229	0.7839039538091228
1103	230-234	1.6088653093151528
1103	235-239	0.9122325946052321
1103	240-244	0.524286642208553
1103	245-249	0.9732605825782503
1103	250-254	0.9667315180237299
1103	255-259	0.7296296856394608
1103	260-264	0.5600789794498535
1103	265-269	0.5131044103888467
1103	270-274	0.5959200579800115
1103	275-279	0.39320969549510565
1103	280-284	0.4894378907619661
1103	285-289	0.4305482697152012
1103	290-294	0.10444704321020026
1103	295-299	0.25183301660910296
1103	300-301	-0.006454695197339433
2116	1	0.07177456400578563
2116	2	0.1174531637924261
2116	3	0.07515124631961356
2116	4	0.14343769192582556
2116	5	0.0776074580906112
2116	6	0.22808155485874693
2116	7	0.9666297453186772
2116	8	0.35275429172970263
2116	9	0.2672204549455728
2116	10-14	0.11963122172861063
2116	15-19	0.24028408376118904
2116	20-24	0.23302269513121132
2116	25-29	0.08344511589601211
2116	30-34	0.4569285372730647
2116	35-39	0.016442407504612788
2116	40-44	0.5375113466596275
2116	45-49	0.22350750691119003
2116	50-54	0.31952305246943524
2116	55-59	0.5514413897081454
2116	60-64	0.3439362256094327
2116	65-69	0.3883214528655117
2116	70-74	0.4337520150072294
2116	75-79	0.5547411667283981
2116	80-84	0.9960245176835159
2116	85-89	0.6561492313696178
2116	90-94	0.8266806357201588
2116	95-99	1.37629576343587
2116	100-104	0.6463599186109263
2116	105-109	0.6175841461694844
2116	110-114	0.7356973516374907
2116	115-119	0.9061402513250982
2116	120-124	1.0286041775620305
2116	125-129	1.022277417958847
2116	130-134	1.259996847509477
2116	135-139	1.208257418776455
2116	140-144	1.8714603677866606
2116	145-149	1.9128871583784601
2116	150-154	2.407608539111166
2116	155-159	2.1978183666131628
2116	160-164	1.7848669487138054
2116	165-169	1.7676855909623228
2116	170-174	1.7118143527919578
2116	175-179	1.9855069304330293
2116	180-184	2.0749150644583843
2116	185-189	1.6522023899967841
2116	190-194	1.5197291611226937
2116	195-199	0.9148285070330076
2116	200-204	1.0179801766431247
2116	205-209	1.005451853555936
2116	210-214	2.07770802922791
2116	215-219	2.0671055186238227
2116	220-224	1.413698992066184
2116	225-229	1.0047300568102457
2116	230-234	1.2266971536720632
2116	235-239	0.8212397434084728
2116	240-244	0.8230384766103409
2116	245-249	0.9875957782048648
2116	250-254	1.0022474247545894
2116	255-259	1.4785973572579412
2116	260-264	0.5745135545981075
2116	265-269	0.7033348369999004
2116	270-274	0.6077249377033258
2116	275-279	0.5752496361076886
2116	280-284	0.2367023793178724
2116	285-289	0.12367220232936127
2116	290-294	0.17750334969046477
2116	295-299	0.09659825608972383
2116	300-301	0.027336916759098884
1117	1	0.30737908200992337
1117	2	0.1342164210715282
1117	3	0.07883735293476235
1117	4	0.05754340208437014
1117	5	0.09830743546578447
1117	6	0.20251723943383837
1117	7	0.8173267640624786
1117	8	0.360580741894168
1117	9	0.1681480728992426
1117	10-14	0.12027133032778181
1117	15-19	0.20913491652888894
1117	20-24	0.2602487171643233
1117	25-29	0.27923341711949945
1117	30-34	0.5206884356353711
1117	35-39	0.0768862022104102
1117	40-44	0.5215799869074615
1117	45-49	0.12637598704485242
1117	50-54	0.1980597482003077
1117	55-59	0.3335026855886767
1117	60-64	0.3672248313979125
1117	65-69	0.48598970327014257
1117	70-74	0.48745709167059204
1117	75-79	0.515546610610123
1117	80-84	0.9633246795380614
1117	85-89	0.4818670475515887
1117	90-94	0.5305794157198065
1117	95-99	1.0820657907596996
1117	100-104	0.3889095627049741
1117	105-109	0.574170929789112
1117	110-114	0.867237406285156
1117	115-119	0.6783231991661722
1117	120-124	0.9254373977712262
1117	125-129	0.9953542205485135
1117	130-134	0.9800100743320783
1117	135-139	1.3083587083916512
1117	140-144	1.3332198832672688
1117	145-149	1.8091860845693049
1117	150-154	2.240607373062332
1117	155-159	1.484115378395476
1117	160-164	2.0290904820158104
1117	165-169	1.5754814105902284
1117	170-174	1.173802553073898
1117	175-179	1.4689667887667994
1117	180-184	1.4426687670985245
1117	185-189	1.4124905954998397
1117	190-194	-0.6307586649793926
1117	195-199	1.0034475223308732
1117	200-204	1.473951216863103
1117	205-209	1.3946075993920957
1117	210-214	1.786577658006692
1117	215-219	1.5994558901931164
1117	220-224	1.8937250976371125
1117	225-229	1.4387828596783798
1117	230-234	1.1213322848090463
1117	235-239	1.0922465482606292
1117	240-244	0.9022326846543418
1117	245-249	1.200021333818432
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2108	230-234	-1.0602710568578821
2108	235-239	-0.8835068186128332
2108	240-244	-0.8402964057496884
2108	245-249	-1.2496232025355685
2108	250-254	-0.7157340213478864
2108	255-259	-0.6412442977910242
2108	260-264	-0.41449042123707613
2108	265-269	-0.5399129556370568
2108	270-274	-0.5194480885784927
2108	275-279	-0.28147537723115335
2108	280-284	-0.7232053675367407
2108	285-289	-0.9383415323739985
2108	290-294	-0.7439434412425676
2108	295-299	-0.5658612540736137
2108	300-301	2.5819659248656706
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	36.0
11	251.0
12	611.0
13	669.0
14	1083.0
15	1893.0
16	3076.0
17	3970.0
18	4392.0
19	5616.0
20	7185.0
21	9361.0
22	12312.0
23	17209.0
24	22989.0
25	31633.0
26	43019.0
27	55699.0
28	63390.0
29	58677.0
30	40358.0
31	18210.0
32	4481.0
33	475.0
34	19.0
35	6.0
36	1.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.83750384260682	19.221395634798647	13.435474946203504	36.50562557639102
2	51.076052874269905	22.166615431909005	13.880602520750077	12.876729173071011
3	50.842176452505385	20.91656932062711	16.09738702735936	12.143867199508145
4	50.89505072241008	19.927205656317245	16.32978788810329	12.847955733169384
5	29.863141715339687	19.807931140485703	37.84223793421457	12.486689209960037
6	28.76065170611743	19.577497694435905	37.926590839225334	13.735259760221332
7	15.359852443897939	53.39022440823855	17.750261297264064	13.499661850599445
8	27.961881340301257	18.34319090070704	39.44002459268368	14.254903166308022
9	14.337780510298186	17.10273593605902	40.322164156163545	28.23731939747925
10-14	27.940436520135258	35.55256071318782	22.31672917307101	14.190273593605902
15-19	21.220633261604675	38.08673839532739	19.269794036274206	21.422834306793728
20-24	27.681573931755306	30.99096218874885	24.976968951736858	16.35049492775899
25-29	17.42022748232401	26.478303104826313	39.878413771902856	16.223055640946818
30-34	18.41426375653243	29.537485398094066	30.20739010144482	21.84086074392868
35-39	20.649886258837995	32.08784506609283	27.631995081463263	19.630273593605903
40-44	19.497473101752227	31.656046726098985	28.04638180141408	20.800098370734705
45-49	20.16678758069474	30.261346449431294	26.791613894866277	22.780252075007684
50-54	22.73898555179834	30.97251767599139	25.13042729787888	21.158069474331388
55-59	21.722422379342145	26.712671380264368	29.601377190285888	21.963529050107596
60-64	20.0459391331079	28.138555179834	28.377546879803255	23.43795880725484
65-69	18.242803565939134	28.60158622809714	28.78829388256994	24.36731632339379
70-74	20.286209652628344	26.493944051644636	30.429609591146633	22.790236704580387
75-79	21.383436827543807	25.60280356593913	28.132210267445434	24.881549339071626
80-84	23.16276667691362	28.230335075315093	24.885877651398708	23.72102059637258
85-89	20.867285582539193	28.382268675069167	25.81607131878266	24.934374423608975
90-94	22.21211189671073	30.528570550261296	27.433138641254228	19.82617891177375
95-99	24.39087611435598	27.561709191515526	27.324242237934214	20.723172456194284
100-104	22.904297571472487	27.127549953888717	27.87865969873963	22.08949277589917
105-109	25.47551183522902	28.118290808484474	23.489013218567475	22.91718413771903
110-114	20.469031663080234	27.236052874269905	30.192732861973564	22.102182600676297
115-119	24.416550876114357	26.21717798954811	27.86237934214571	21.503891792191823
120-124	19.55049492775899	28.493575161389483	27.83321241930526	24.122717491546265
125-129	19.724463572087302	24.993593605902245	29.622772825084535	25.65916999692591
130-134	23.725693206271135	28.993642791269597	25.819907777436214	21.460756225023054
135-139	22.134841684598833	26.567869658776516	25.42027666769136	25.87701198893329
140-144	21.93283738087919	28.044217645250534	26.378014140793116	23.64493083307716
145-149	22.433249308330772	24.21572702121119	29.177891177374732	24.17313249308331
150-154	21.201844451275743	26.953532124193053	26.055899169996927	25.78872425453428
155-159	21.989449738702735	27.2482508453735	29.820399631109744	20.941899784814016
160-164	23.32291423301568	29.378665846910547	24.137866584691054	23.160553335382726
165-169	19.721463264678757	28.08086074392868	27.435007685213648	24.762668306178913
170-174	21.001315708576698	29.615050722410086	27.085152167230248	22.29848140178297
175-179	19.47671687672917	27.892972640639407	28.068072548416843	24.562237934214572
180-184	20.812689824777127	27.910236704580388	28.198807254841686	23.0782662158008
185-189	20.837725176759914	26.784973870273593	28.434159237626805	23.943141715339685
190-194	19.8644943129419	26.07739317553028	28.989068552105753	25.06904395942207
195-199	18.470482631417156	31.817030433446046	26.904936981248078	22.807549953888717
200-204	20.420338149400553	27.41454657239471	25.84297571472487	26.322139563479862
205-209	22.133661235782355	25.122065785428838	28.864482016600064	23.87979096218875
210-214	22.86671995081463	26.230900707039655	28.53007070396557	22.37230863818014
215-219	22.048078696587766	26.88477098063326	29.36932062711343	21.69782969566554
220-224	20.46908084844759	26.429019366738395	27.622649861666154	25.47924992314786
225-229	23.321537042729787	26.503240086074392	28.256501690747005	21.918721180448816
230-234	21.75877036581617	27.070249000922225	27.91230249000922	23.258678143252382
235-239	21.69478020288964	26.589806332616046	26.118659698739627	25.596753765754688
240-244	22.54130956040578	29.498432216415615	25.437393175530282	22.522865047648324
245-249	23.20093452197971	26.883492161082078	25.63934829388257	24.27622502305564
250-254	21.01877651398709	26.85845680909929	24.371644635720873	27.751122041192744
255-259	22.195044574239166	28.00167230249001	24.83211804488165	24.97116507838918
260-264	20.690906855210574	26.50874884721795	27.409923147863513	25.390421149707965
265-269	21.54397786658469	27.054263756532432	25.84543498309253	25.556323393790347
270-274	20.6323270826929	29.625182908084845	24.424764832462344	25.317725176759915
275-279	21.316200430371964	28.366775284352908	24.946228097141102	25.370796188134033
280-284	19.0131939747925	29.102490009222254	27.830212111896714	24.054103904088535
285-289	20.73920688595143	28.91745465723947	24.904666461727636	25.438671995081464
290-294	21.222403934829387	28.76876729173071	25.355696280356593	24.65313249308331
295-299	19.777239471257303	29.053403012603752	24.72745158315401	26.441905932984938
300-301	16.00639409775592	29.98094067015063	30.961573931755304	23.05109130033815
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.25
17	0.25
18	0.0
19	0.0
20	0.25
21	0.25
22	0.25
23	0.25
24	0.0
25	0.0
26	0.75
27	0.25
28	0.5
29	1.5
30	3.25
31	7.75
32	29.0
33	87.5
34	181.75
35	359.5
36	718.0
37	1485.5
38	2766.75
39	4763.75
40	6946.5
41	8365.5
42	9765.75
43	11803.0
44	13898.75
45	14502.5
46	13443.5
47	12098.0
48	10343.5
49	8131.25
50	6153.5
51	4262.75
52	2423.75
53	1117.0
54	478.5
55	234.5
56	140.0
57	110.0
58	100.0
59	103.75
60	134.0
61	143.25
62	126.5
63	98.75
64	58.25
65	34.25
66	21.5
67	18.25
68	11.0
69	9.25
70	5.5
71	3.75
72	4.5
73	3.0
74	3.25
75	1.5
76	2.25
77	2.0
78	1.5
79	0.75
80	0.75
81	0.5
82	1.5
83	2.0
84	1.25
85	1.25
86	1.5
87	1.0
88	0.25
89	1.0
90	0.75
91	1.0
92	1.5
93	0.75
94	1.75
95	0.5
96	0.5
97	1.5
98	1.25
99	1.0
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	406625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.642875733412414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.89072075647039	7.215815307311421
2	6.575473408974364	1.794167332139899
3	4.72466472091971	1.9337404100863573
4	4.762522931952975	2.5989803415264547
5	3.828939871984818	2.611887543209846
6	3.314273027969925	2.7129729040316497
7	2.901862242529035	2.7712822179213568
8	2.529936238347351	2.76124845705838
9	2.098944409055741	2.5772073969679403
>10	15.974256995006266	38.74975093123966
>50	0.30808329615017943	2.681396072549181
>100	0.04341699310889511	1.089609929002326
>500	0.011257225807482141	1.1439219810922707
>1k	0.01313343010842513	4.260847012061833
>5k	0.016885838710832306	15.226269645109824
>10k+	0.005628612903610769	9.870902518691606
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	13785	3.3901014448201665	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	13002	3.1975407316323397	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	11776	2.8960344297571474	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	9826	2.416477098063326	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	7023	1.727144174608054	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	6782	1.667875806947433	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	6759	1.6622194897018137	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC	6316	1.5532739010144483	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	6213	1.5279434368275437	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	5750	1.414079311404857	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	5748	1.413587457731325	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	5068	1.2463572087304027	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	3787	0.9313249308330771	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	3227	0.7936059022440825	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	2819	0.693267752843529	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	2666	0.6556409468183215	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC	1526	0.3752843529050108	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1383	0.3401168152474639	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1238	0.30445742391638486	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	897	0.2205963725791577	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	859	0.21125115278204737	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	763	0.1876421764525054	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	741	0.182231786043652	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	609	0.14976944359053182	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	600	0.14755610205963726	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	467	0.11484783276975101	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	427	0.10501075929910851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	2.459268367660621E-4	0.0	0.0
4	2.459268367660621E-4	0.0	0.0
5	2.459268367660621E-4	0.0	0.0
6	2.459268367660621E-4	0.0	0.0
7	2.459268367660621E-4	0.0	0.0
8	2.459268367660621E-4	0.0	0.0
9	2.459268367660621E-4	0.0	0.0
10-14	0.002213341530894559	0.0	0.0
15-19	0.002803565939133108	0.0	0.0
20-24	0.003541346449431294	0.0	0.0
25-29	0.003787273286197356	0.0	0.0
30-34	0.00590224408238549	0.0	0.0
35-39	0.005951429449738702	0.0	0.0
40-44	0.0065416538579772514	0.0	0.0
45-49	0.009837073470642483	0.0	0.0
50-54	0.012739010144482015	0.0	0.0
55-59	0.015001537042729787	0.0	0.0
60-64	0.018788810328927145	0.0	0.0
65-69	0.023313864125422686	0.0	0.0
70-74	0.030396557024285276	0.0	0.0
75-79	0.040381186596987396	0.0	0.0
80-84	0.05587457731324931	0.0	0.0
85-89	0.07161389486627727	0.0	0.0
90-94	0.08941899784814018	0.0	0.0
95-99	0.10830617891177374	0.0	0.0
100-104	0.13407931140485707	0.0	0.0
105-109	0.1651152782047341	0.0	0.0
110-114	0.2031355671687673	0.0	0.0
115-119	0.2521733784199201	0.0	0.0
120-124	0.3117860436520135	0.0	0.0
125-129	0.38384260682446975	0.0	0.0
130-134	0.4680971411005226	0.0	0.0
135-139	0.563123270826929	0.0	0.0
140-144	0.6744789425146019	0.0	0.0
145-149	0.8115093759606516	0.0	0.0
150-154	0.9758868736550876	0.0	0.0
155-159	1.174890869966185	0.0	0.0
160-164	1.4013894866277283	0.0	0.0
165-169	1.683762680602521	0.0	0.0
170-174	2.0062711343375343	0.0	0.0
175-179	2.3598647402397783	0.0	0.0
180-184	2.747199508146326	0.0	0.0
185-189	3.1829326775284352	0.0	0.0
190-194	3.652603750384261	0.0	0.0
195-199	4.155032277897325	0.0	0.0
200-204	4.6460498001844455	0.0	0.0
205-209	5.175530279741777	0.0	0.0
210-214	5.724980018444513	0.0	0.0
215-219	6.241623117122655	0.0	0.0
220-224	6.784875499538887	0.0	0.0
225-229	7.295124500461112	0.0	0.0
230-234	7.781666154319089	0.0	0.0
235-239	8.188134030126037	0.0	0.0
240-244	8.573009529664924	0.0	0.0
245-249	8.88435290501076	0.0	0.0
250-254	9.155806947433138	0.0	1.9674146941284966E-4
255-259	9.425982170304334	0.0	2.459268367660621E-4
260-264	9.642004303719643	0.0	2.459268367660621E-4
265-269	9.783904088533662	0.0	2.459268367660621E-4
270-274	9.898948662772824	0.0	2.459268367660621E-4
275-279	9.978284660313557	0.0	2.459268367660621E-4
280-284	10.03022440823855	0.0	2.459268367660621E-4
285-289	10.072720565631723	0.0	2.459268367660621E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8415	0.0	292.8966	1
GGGTTAT	8495	0.0	291.18008	2
GGTTATT	8730	0.0	282.6661	3
ATTAGTA	8740	0.0	280.65503	7
TTAGTAG	8835	0.0	280.47537	8
GAGCGAA	4645	0.0	278.17007	6
TTATTAG	8810	0.0	277.92282	5
GGGGGGA	5890	0.0	277.21988	1
GGAGCGA	4700	0.0	276.1702	5
CGAAAGC	4780	0.0	275.5596	9
GTTATTA	8970	0.0	274.28094	4
GCGAAAG	4805	0.0	273.81894	8
TAGTAGA	9190	0.0	269.4804	9
TATTAGT	9165	0.0	267.8014	6
GGGGGAG	6355	0.0	257.86386	2
AGCGAAA	5530	0.0	233.91954	7
GGGAGCG	5640	0.0	231.711	4
GGGGAGC	7215	0.0	226.51419	3
GCAAAAG	2005	0.0	150.81047	8
GAGCAAA	2130	0.0	141.96008	6
>>END_MODULE