FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6955_flu.3520000003bb4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6955_flu.3520000003bb4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406625
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA137853.3901014448201665No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC130023.1975407316323397No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC117762.8960344297571474No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG98262.416477098063326No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG70231.727144174608054No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA67821.667875806947433No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG67591.6622194897018137No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC63161.5532739010144483No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT62131.5279434368275437No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG57501.414079311404857No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA57481.413587457731325No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA50681.2463572087304027No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA37870.9313249308330771No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT32270.7936059022440825No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA28190.693267752843529No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA26660.6556409468183215No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC15260.3752843529050108No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13830.3401168152474639No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG12380.30445742391638486No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT8970.2205963725791577Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8590.21125115278204737No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT7630.1876421764525054No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7410.182231786043652No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6090.14976944359053182No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6000.14755610205963726No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4670.11484783276975101No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG4270.10501075929910851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA84150.0292.89661
GGGTTAT84950.0291.180082
GGTTATT87300.0282.66613
ATTAGTA87400.0280.655037
TTAGTAG88350.0280.475378
GAGCGAA46450.0278.170076
TTATTAG88100.0277.922825
GGGGGGA58900.0277.219881
GGAGCGA47000.0276.17025
CGAAAGC47800.0275.55969
GTTATTA89700.0274.280944
GCGAAAG48050.0273.818948
TAGTAGA91900.0269.48049
TATTAGT91650.0267.80146
GGGGGAG63550.0257.863862
AGCGAAA55300.0233.919547
GGGAGCG56400.0231.7114
GGGGAGC72150.0226.514193
GCAAAAG20050.0150.810478
GAGCAAA21300.0141.960086
Produced by FastQC (version 0.11.2)