##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6929_flu.3520000003bb82.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397083
Sequences flagged as poor quality	0
Sequence length	301
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.008736208802695	34.0	34.0	34.0	33.0	34.0
2	33.408803700989466	34.0	34.0	34.0	34.0	34.0
3	33.418076321575086	34.0	34.0	34.0	34.0	34.0
4	33.4053787243473	34.0	34.0	34.0	34.0	34.0
5	33.356426742016154	34.0	34.0	34.0	34.0	34.0
6	36.90089981187812	38.0	38.0	38.0	36.0	38.0
7	35.97238864418774	38.0	38.0	38.0	34.0	38.0
8	36.51238154239794	38.0	37.0	38.0	34.0	38.0
9	36.78533707058726	38.0	38.0	38.0	36.0	38.0
10-14	36.80076860505234	38.0	38.0	38.0	35.6	38.0
15-19	36.769919638967174	38.0	38.0	38.0	35.6	38.0
20-24	36.834483470710154	38.0	38.0	38.0	35.8	38.0
25-29	36.739355751820156	38.0	38.0	38.0	35.4	38.0
30-34	36.400325372781005	38.0	37.4	38.0	33.2	38.0
35-39	36.691931913479046	38.0	38.0	38.0	34.8	38.0
40-44	36.16592500812173	38.0	37.2	38.0	33.0	38.0
45-49	36.47559678958807	38.0	37.4	38.0	34.0	38.0
50-54	36.42602931880741	38.0	37.2	38.0	34.0	38.0
55-59	36.058525799392065	38.0	37.0	38.0	32.8	38.0
60-64	36.214977725059995	38.0	37.0	38.0	33.6	38.0
65-69	36.056963909308635	38.0	37.0	38.0	32.6	38.0
70-74	35.961117952669845	38.0	37.0	38.0	32.0	38.0
75-79	35.98022075989151	38.0	37.0	38.0	32.0	38.0
80-84	35.21164542425639	38.0	36.4	38.0	28.2	38.0
85-89	35.3892878818786	38.0	37.0	38.0	28.8	38.0
90-94	35.352304681892704	38.0	36.6	38.0	28.4	38.0
95-99	34.43209807521349	38.0	34.8	38.0	24.0	38.0
100-104	34.73464943097539	38.0	35.6	38.0	24.8	38.0
105-109	34.430857024853744	38.0	34.8	38.0	24.0	38.0
110-114	33.989362929160905	37.2	34.0	38.0	23.0	38.0
115-119	33.99334597552653	37.2	34.0	38.0	23.2	38.0
120-124	33.36624383315326	37.0	32.8	38.0	21.6	38.0
125-129	32.88973690638985	37.0	31.0	38.0	20.8	38.0
130-134	32.70352948879705	37.0	30.8	38.0	20.4	38.0
135-139	31.999890702951273	36.4	28.4	38.0	17.6	38.0
140-144	30.60040545679367	35.4	25.6	37.8	13.6	38.0
145-149	30.23328221052022	35.0	24.4	37.6	11.2	38.0
150-154	29.19255269049544	34.0	22.8	37.0	10.0	38.0
155-159	29.102257210709098	33.8	23.2	37.0	10.0	37.0
160-164	28.273872716787167	32.6	21.4	37.0	10.0	37.2
165-169	26.966056970457053	30.0	20.0	36.0	9.2	37.0
170-174	26.222835024415552	28.8	19.4	35.4	9.0	37.0
175-179	25.42100971333449	27.0	17.4	35.0	9.0	37.0
180-184	24.392383960028507	25.6	15.6	35.0	8.0	36.6
185-189	23.654041598356013	24.6	15.0	34.4	8.0	36.2
190-194	22.652688732582355	23.4	13.0	32.8	8.0	36.0
195-199	21.836648257417217	22.4	11.4	31.4	8.0	36.0
200-204	21.125846233658958	21.2	10.4	29.8	8.0	35.0
205-209	20.190331492408387	20.2	9.0	28.6	8.0	35.0
210-214	19.07551166884505	18.4	9.0	26.6	8.0	34.2
215-219	18.623827260295705	17.6	9.0	25.8	7.4	33.6
220-224	18.018668137391934	16.8	9.0	25.0	7.4	32.2
225-229	17.332033353228418	15.6	8.2	24.6	7.2	31.6
230-234	16.61331510036944	14.8	8.0	23.2	7.0	30.2
235-239	15.710226829151589	13.4	8.0	21.6	7.0	28.6
240-244	14.96283447037521	12.6	8.0	20.2	7.0	27.6
245-249	14.384661141373465	12.0	8.0	19.4	7.0	26.0
250-254	13.693625262224774	11.0	7.8	18.4	7.0	25.0
255-259	13.23285358476691	10.8	8.0	17.4	7.0	24.0
260-264	12.687815393758987	9.8	7.0	16.2	7.0	22.8
265-269	12.332177907389639	9.0	7.0	15.6	7.0	21.8
270-274	11.926586129348271	8.8	7.0	14.2	7.0	21.0
275-279	11.521024068016006	8.0	7.0	13.4	7.0	20.2
280-284	11.369939282215556	8.0	7.0	13.4	7.0	19.6
285-289	11.087325319895337	8.0	7.0	13.0	7.0	19.0
290-294	10.901152655741999	8.0	7.0	13.0	7.0	19.0
295-299	10.641613969875316	8.0	7.0	13.0	7.0	18.4
300-301	10.392727716875314	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.36082298271779933
1101	2	0.1311127084889563
1101	3	0.19270383148356984
1101	4	0.0485505391261114
1101	5	0.18456491750022508
1101	6	0.3232429081490267
1101	7	1.0630159677907614
1101	8	0.5365653375931032
1101	9	0.22325851463637747
1101	10-14	0.18846620776999856
1101	15-19	0.26432255838617635
1101	20-24	0.24711384357070187
1101	25-29	0.16733303272753375
1101	30-34	0.5554197423279845
1101	35-39	0.3017725603564685
1101	40-44	0.5514249078878208
1101	45-49	0.14900347247327517
1101	50-54	0.20922299080151419
1101	55-59	0.30277225334826596
1101	60-64	0.28644152045921345
1101	65-69	0.31274201375845934
1101	70-74	0.2417483783909944
1101	75-79	0.34434159283642884
1101	80-84	0.7301726059103686
1101	85-89	0.39534097542330215
1101	90-94	0.24442033572004362
1101	95-99	0.8968192684969765
1101	100-104	0.38269957650111763
1101	105-109	0.6399619374696144
1101	110-114	0.4256274838647869
1101	115-119	0.48873398693805115
1101	120-124	0.8928445202597004
1101	125-129	0.631882025515246
1101	130-134	0.7503243534095034
1101	135-139	0.6328712238337033
1101	140-144	1.1430314003619237
1101	145-149	1.3307630941765005
1101	150-154	1.1249128439118579
1101	155-159	0.684157160323533
1101	160-164	0.8370498277452292
1101	165-169	0.6083670334439901
1101	170-174	0.23127264853684792
1101	175-179	0.8184024981680089
1101	180-184	0.5131905631219773
1101	185-189	0.22621124840773632
1101	190-194	0.8738183670780089
1101	195-199	1.0276520702443257
1101	200-204	0.7109804946009852
1101	205-209	0.545204011164703
1101	210-214	0.6186778419170693
1101	215-219	0.6970761664777214
1101	220-224	0.44746175845356007
1101	225-229	0.6488161922176268
1101	230-234	0.5660081756999951
1101	235-239	0.22436371765048513
1101	240-244	0.20045907472379554
1101	245-249	0.23639445009283477
1101	250-254	0.25642980301785734
1101	255-259	0.13808224248250411
1101	260-264	0.1665975474787995
1101	265-269	0.045526742500539896
1101	270-274	-0.051065938726043925
1101	275-279	-0.055822400466384536
1101	280-284	-0.02554232815623081
1101	285-289	-0.01576117336811933
1101	290-294	-0.047508185507330936
1101	295-299	0.028457470515371952
1101	300-301	-0.2006855788876276
1102	1	-0.15820585237116092
1102	2	-0.1235502182467556
1102	3	0.022838491127728844
1102	4	0.013433361999439342
1102	5	0.03625028382166562
1102	6	0.027997673425943503
1102	7	0.8975811915964869
1102	8	0.18271217598677936
1102	9	0.09805104805232645
1102	10-14	0.08381397708929228
1102	15-19	0.1816606791261961
1102	20-24	0.06411669831089739
1102	25-29	0.03328306370921297
1102	30-34	0.37856453002972756
1102	35-39	-0.006409552888932524
1102	40-44	0.4300322446438898
1102	45-49	-0.015354690460824827
1102	50-54	0.005218609107259908
1102	55-59	0.18538821333764588
1102	60-64	0.10345599332811162
1102	65-69	0.2504539062962934
1102	70-74	0.04181698067478834
1102	75-79	0.14959317142661632
1102	80-84	0.4672632051845156
1102	85-89	0.06438161305570844
1102	90-94	0.07149481714578343
1102	95-99	0.8562432306108079
1102	100-104	0.4446138752825135
1102	105-109	0.6242833649872495
1102	110-114	0.26537623722113324
1102	115-119	0.23569261902326843
1102	120-124	0.6650985847605995
1102	125-129	0.6952517254355897
1102	130-134	0.7115725461450495
1102	135-139	0.8117478061121801
1102	140-144	1.4242011799494207
1102	145-149	1.4747419528705485
1102	150-154	1.0336963336288925
1102	155-159	0.8476435947751568
1102	160-164	1.14791609755137
1102	165-169	1.0868157735224777
1102	170-174	1.283450586632508
1102	175-179	0.8107179358948393
1102	180-184	0.8442792002232729
1102	185-189	0.8162454757837381
1102	190-194	1.1716797093222588
1102	195-199	1.1317543761662527
1102	200-204	1.2275400163029175
1102	205-209	0.5744315346960285
1102	210-214	1.1926185636426005
1102	215-219	0.971296092121694
1102	220-224	0.6855402674972559
1102	225-229	0.5940984943037257
1102	230-234	0.9160435833303389
1102	235-239	0.5501583607710767
1102	240-244	0.5763750627442121
1102	245-249	0.18857165052952496
1102	250-254	0.5703291420754244
1102	255-259	0.36354383258016654
1102	260-264	0.2814820300848009
1102	265-269	0.15555097459755807
1102	270-274	0.3096083553343245
1102	275-279	0.31208722155716195
1102	280-284	0.44621252563976377
1102	285-289	0.2940779063285568
1102	290-294	0.10729305228441355
1102	295-299	0.09564086728737564
1102	300-301	-0.06645700419228007
1103	1	-0.08505147716527972
1103	2	-0.03576841636630235
1103	3	-0.04335109099376666
1103	4	0.004205351461955331
1103	5	-0.12036726781988705
1103	6	0.004532446119597466
1103	7	0.6626022311701263
1103	8	0.054800461431675274
1103	9	-0.015549945077253824
1103	10-14	-0.17157096498269198
1103	15-19	-0.08305046118511683
1103	20-24	0.03770892532956793
1103	25-29	-0.1521778966971894
1103	30-34	0.2254301916487691
1103	35-39	0.00430016633234942
1103	40-44	0.3875850296082817
1103	45-49	-0.04983545265874767
1103	50-54	-0.09090515458525061
1103	55-59	0.06388701230785188
1103	60-64	0.08109859916802264
1103	65-69	0.30816213999754893
1103	70-74	0.028880887075224848
1103	75-79	0.2974126693988879
1103	80-84	0.6501113926594968
1103	85-89	0.33096521643548726
1103	90-94	0.2796456668505414
1103	95-99	1.0299496167135374
1103	100-104	0.6586721399736888
1103	105-109	0.6015370313722954
1103	110-114	0.5885232165881007
1103	115-119	0.6241663628876708
1103	120-124	0.9650885119002481
1103	125-129	1.014561217246161
1103	130-134	0.9213680852290977
1103	135-139	1.0936995090154298
1103	140-144	1.5799648071229093
1103	145-149	1.3899870909004939
1103	150-154	1.8169316456289337
1103	155-159	0.6745383134202001
1103	160-164	0.9869031489347613
1103	165-169	1.6475341590335226
1103	170-174	2.0727363300867374
1103	175-179	1.7419865445968838
1103	180-184	1.4363619319829972
1103	185-189	1.3748500125637406
1103	190-194	1.2849710754289951
1103	195-199	1.2006403994826549
1103	200-204	1.2552999119807495
1103	205-209	1.2305566051379877
1103	210-214	1.5877612670349777
1103	215-219	1.6714629749162562
1103	220-224	1.3146143538389126
1103	225-229	0.8678448996084036
1103	230-234	1.5585566803174622
1103	235-239	0.986087255451606
1103	240-244	0.7413429248748855
1103	245-249	1.223737004245729
1103	250-254	0.5430458469614869
1103	255-259	0.8908175993126868
1103	260-264	0.51506403904874
1103	265-269	0.4389929163546462
1103	270-274	0.3341507434733977
1103	275-279	0.409230975793891
1103	280-284	0.3942035344775636
1103	285-289	0.37606064453128596
1103	290-294	0.23758758816603098
1103	295-299	0.08931311456067093
1103	300-301	-0.23987626840159315
2116	1	0.14252623317105417
2116	2	0.059507230633478514
2116	3	0.1241523983488051
2116	4	0.07166361432251733
2116	5	0.1281757048610146
2116	6	0.12542876241821688
2116	7	0.9567611115359043
2116	8	0.24042552061995082
2116	9	0.1796142730754653
2116	10-14	0.14074594963306453
2116	15-19	0.27257873122567844
2116	20-24	0.20551138738490948
2116	25-29	0.1501667113945473
2116	30-34	0.5917661120076829
2116	35-39	0.09733502258559668
2116	40-44	0.5011707638836782
2116	45-49	0.283725694695498
2116	50-54	0.28758243999429567
2116	55-59	0.4010445057871834
2116	60-64	0.38884914370017043
2116	65-69	0.42126905770217604
2116	70-74	0.48976918474513553
2116	75-79	0.4867059414507722
2116	80-84	1.0646185611896612
2116	85-89	0.5783489396812698
2116	90-94	0.7143164118661076
2116	95-99	1.3602041860485627
2116	100-104	0.6482514628863427
2116	105-109	0.7290569473979716
2116	110-114	0.9450978283351361
2116	115-119	0.8263041328415284
2116	120-124	1.0487378553197075
2116	125-129	1.1729778124443726
2116	130-134	1.109377491629317
2116	135-139	1.387566143111961
2116	140-144	2.0738459561764735
2116	145-149	2.302181336428074
2116	150-154	2.270747795996808
2116	155-159	2.059888296471332
2116	160-164	2.10106983551524
2116	165-169	1.6630187860124082
2116	170-174	1.6611105575414271
2116	175-179	2.0848112437434168
2116	180-184	2.0770535519849602
2116	185-189	1.4669649391614286
2116	190-194	1.6303403173499618
2116	195-199	1.185897451406376
2116	200-204	0.8023293463665055
2116	205-209	0.7418326911267208
2116	210-214	1.8899655048713981
2116	215-219	1.7619244354926558
2116	220-224	1.2314981695733032
2116	225-229	0.9358586250933918
2116	230-234	1.1743868340786534
2116	235-239	1.0860257438958438
2116	240-244	0.8177036357183542
2116	245-249	1.1246433925084407
2116	250-254	0.9697521288401827
2116	255-259	1.5756971738474341
2116	260-264	0.604320935202189
2116	265-269	0.8648012180250149
2116	270-274	0.46744070894726875
2116	275-279	0.5341219144779306
2116	280-284	0.3753312581340218
2116	285-289	0.21292580323552457
2116	290-294	0.11139225047643819
2116	295-299	0.1265807978053637
2116	300-301	-0.14992557500262294
1117	1	0.2627156606868368
1117	2	0.22213622865734095
1117	3	0.19463110853573085
1117	4	0.1011005996745098
1117	5	0.19950649550674626
1117	6	0.16260495151320953
1117	7	0.9366500248215814
1117	8	0.4369217802307048
1117	9	0.3051529225595644
1117	10-14	0.2742108449121119
1117	15-19	0.24981851461833315
1117	20-24	0.2738702876499346
1117	25-29	0.3031463274252246
1117	30-34	0.5457031664839107
1117	35-39	0.23943077908033672
1117	40-44	0.6247556823306297
1117	45-49	0.3721282742493628
1117	50-54	0.380095968917594
1117	55-59	0.5695668645410734
1117	60-64	0.5638839994835223
1117	65-69	0.5656423900234842
1117	70-74	0.5518750975329354
1117	75-79	0.6310362631359965
1117	80-84	1.176242366311719
1117	85-89	0.8456222731772485
1117	90-94	0.7235839389262182
1117	95-99	1.3245774641547925
1117	100-104	0.8618402938554439
1117	105-109	0.952199561089266
1117	110-114	1.0201125341776205
1117	115-119	1.0185490260061911
1117	120-124	1.3292474409989694
1117	125-129	1.5566106389569967
1117	130-134	1.2522637041000522
1117	135-139	1.5706031458127683
1117	140-144	1.7321651258182023
1117	145-149	2.148878237041888
1117	150-154	2.533102194602389
1117	155-159	2.0625981472623742
1117	160-164	2.2645998882936276
1117	165-169	1.9033056561121562
1117	170-174	1.8659369653491211
1117	175-179	1.8184792648836918
1117	180-184	1.8570975204209397
1117	185-189	1.6479582137798197
1117	190-194	-0.596435972223663
1117	195-199	1.6625274054229457
1117	200-204	1.7044951487543223
1117	205-209	1.7402722001910327
1117	210-214	1.9291534351471853
1117	215-219	1.73374795092543
1117	220-224	1.8417375924356243
1117	225-229	1.4154760111490923
1117	230-234	1.1219592179973752
1117	235-239	0.8553344021356803
1117	240-244	0.7325214323629812
1117	245-249	1.120577983812522
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2108	240-244	-0.778074288999397
2108	245-249	-0.8606653311001864
2108	250-254	-0.6610628165879451
2108	255-259	-0.6269363170382061
2108	260-264	-0.40325137151051926
2108	265-269	-0.5383432974404165
2108	270-274	-0.5632316502828747
2108	275-279	-0.406697194249487
2108	280-284	-0.6897642392441874
2108	285-289	-1.0252911073787114
2108	290-294	-0.8118190954665216
2108	295-299	-0.5612544770404178
2108	300-301	2.486851051745349
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	16.0
11	95.0
12	362.0
13	657.0
14	1140.0
15	2131.0
16	3318.0
17	4666.0
18	4885.0
19	6370.0
20	8516.0
21	11265.0
22	14965.0
23	20342.0
24	27157.0
25	36044.0
26	44406.0
27	51385.0
28	55310.0
29	49817.0
30	34358.0
31	15502.0
32	3900.0
33	445.0
34	22.0
35	4.0
36	3.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.40569603835974	17.967780035911886	12.94439701523359	33.68212691049478
2	52.682940342447296	20.787341689268995	13.586831972156954	12.942885996126755
3	52.28252027913559	19.733154025732656	15.331807204035428	12.65251849109632
4	52.682184832893874	18.635146808098053	15.634514698438363	13.048153660569707
5	34.40313486097365	18.801358909850084	34.06088903327516	12.73461719590111
6	33.39755164537389	18.634391298544635	34.10496042389123	13.863096632190247
7	15.321230070287573	53.61675015047231	17.152333391255734	13.909686387984376
8	32.41362637030545	17.44244905976836	35.68019784276839	14.4637267271578
9	14.216423266672207	16.537348614773233	36.29291609059063	32.953312027963925
10-14	28.12233210688949	36.86629747433156	20.672604971756535	14.33876544702241
15-19	22.819007613017934	35.81563552204451	18.493715419698148	22.87164144523941
20-24	26.312181584202797	29.509850585393988	24.47578969636071	19.702178134042505
25-29	22.26622645643354	24.66144357728737	34.60107836396924	18.47125160230984
30-34	20.99122853408481	27.712291888597594	29.391588156632242	21.90489142068535
35-39	20.774095088432393	28.631344076679184	27.584207835641415	23.010352999247008
40-44	23.252518994769357	27.18444254727601	26.310922401613766	23.252116056340867
45-49	22.40770821213701	28.40655480088546	25.22580921369084	23.959927773286694
50-54	22.92921127320988	28.387465592835753	27.008559923240227	21.674763210714133
55-59	22.93570865536928	25.471652022373153	27.49717313508763	24.095466187169936
60-64	23.256195807929323	25.51431312848951	27.47843649816285	23.751054565418315
65-69	20.777872636199486	28.505123613954765	26.928072972149398	23.78893077769635
70-74	20.858409954593878	24.665875900000756	29.061077910663514	25.414636234741856
75-79	21.598154541997516	25.218505954674463	28.204229342480037	24.979110160847984
80-84	26.111820450636262	25.742325911711156	25.47265936844438	22.6731942692082
85-89	24.245711853693056	27.15296298255025	23.945119786039694	24.656205377717004
90-94	22.681555241599362	28.082390835165445	27.45234623491814	21.78370768831705
95-99	22.587922424279057	27.884396964866287	25.803068879805984	23.72461173104867
100-104	21.957223049085457	28.691986310166897	24.25412319338778	25.09666744735987
105-109	25.301964576675406	26.91160286388488	22.698579390203054	25.087853169236656
110-114	21.11694532377362	27.57564539403601	28.471226418658063	22.836182863532308
115-119	24.697254730119393	27.172203292510634	24.04394043562681	24.086601541743164
120-124	19.71396408307583	26.104920130048377	28.810601310053563	25.370514476822226
125-129	23.015238627692447	24.24379789615773	28.920603501031273	23.820359975118553
130-134	25.4049657124581	26.043774223525055	26.09535034237175	22.4559097216451
135-139	24.122765265700117	26.285134342190425	23.297597731456648	26.294502660652814
140-144	22.175263106201978	25.301561638246916	26.24448792821652	26.278687327334588
145-149	22.934751676601618	25.304331839942783	27.881072722831245	23.879843760624354
150-154	23.34731026007157	24.75316243707235	25.025851018552796	26.873676284303283
155-159	22.525164764041776	25.310678120191497	28.876985416147257	23.287171699619474
160-164	25.945155043152184	26.735871341759783	25.034413460158206	22.284560154929824
165-169	21.97359242274285	24.288574429023654	26.69587970273217	27.04195344550132
170-174	23.68451935741394	27.4465539950086	25.5254946698801	23.343431977697357
175-179	22.586663241690026	27.145861192748118	25.50842015397285	24.759055411589014
180-184	21.268299070975086	27.003573560187665	26.92258293606123	24.80554443277602
185-189	22.59693817161651	25.1118280057318	26.555153456582126	25.736080366069565
190-194	21.899955424936348	25.735627060337514	25.231702188207507	27.132715326518635
195-199	21.341684232263784	28.4940428071713	27.073080439102153	23.091192521462766
200-204	24.574207407519335	26.29601367975965	23.868309648108834	25.261469264612185
205-209	22.840514451638576	25.85147185852832	27.363498311436146	23.94451537839696
210-214	24.528020590153695	25.33017026666969	25.69427550411375	24.447533639062865
215-219	23.910618183100258	24.650010199378972	25.86622947847175	25.573142139049015
220-224	20.167572018948178	25.32034864247525	27.3771982180048	27.13488112057177
225-229	22.458377719519596	26.617911116819403	24.001228962206895	26.922482201454105
230-234	22.21948559872873	24.268276405688482	26.569205934275708	26.943032061307083
235-239	22.862524963294828	24.87298625224449	24.030341263665278	28.2341475207954
240-244	23.316032164560056	28.314987043011158	22.760027500547743	25.608953291881043
245-249	23.208749807974655	26.356706280550917	22.012576715699236	28.42196719577519
250-254	23.157022587217284	24.940831010141455	23.963604586446664	27.9385418161946
255-259	23.033673060795852	25.0144176406444	25.426623652989427	26.525285645570325
260-264	23.322831750540818	26.610255286678104	23.899688478227475	26.167224484553607
265-269	22.202411082821474	26.4499865267463	23.49246882893501	27.85513356149722
270-274	21.034141476718972	27.99142748493388	22.70991203350433	28.264519004842814
275-279	22.075284008632956	27.35710166388387	21.80919354391903	28.75842078356414
280-284	20.436130481536605	27.444740772080394	24.329875617943856	27.78925312843914
285-289	21.677231208588633	26.80411903808524	24.569372146377457	26.949277606948673
290-294	21.77378532951549	28.659902337798393	22.396728139960665	27.16958419272545
295-299	21.09347416031409	29.517254579017482	21.087984124225915	28.301287136442504
300-301	17.530969595777204	29.071126187723976	27.152257840300393	26.24564637619843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.25
3	0.0
4	0.75
5	1.25
6	1.0
7	1.0
8	2.25
9	2.25
10	1.75
11	2.25
12	5.25
13	7.75
14	8.25
15	11.25
16	9.5
17	7.75
18	6.25
19	7.0
20	5.0
21	5.5
22	5.5
23	5.0
24	4.75
25	4.75
26	6.5
27	3.75
28	4.25
29	5.0
30	4.5
31	8.0
32	9.75
33	20.75
34	47.25
35	126.5
36	374.25
37	1008.5
38	2227.25
39	3688.0
40	5015.5
41	6357.75
42	8325.25
43	11016.75
44	12601.0
45	12256.5
46	10602.25
47	8369.75
48	6576.0
49	5256.25
50	4246.5
51	3473.5
52	2632.0
53	1858.75
54	1379.75
55	1310.0
56	1412.75
57	1570.5
58	1804.25
59	2245.0
60	2740.75
61	3131.25
62	3070.75
63	2494.75
64	1752.5
65	1089.75
66	611.75
67	402.75
68	320.0
69	258.0
70	185.5
71	111.5
72	67.25
73	37.5
74	25.0
75	19.0
76	13.5
77	9.75
78	8.0
79	5.75
80	3.0
81	2.5
82	3.25
83	3.5
84	2.25
85	1.0
86	1.5
87	0.75
88	0.25
89	1.0
90	0.75
91	0.75
92	0.0
93	1.0
94	0.25
95	0.5
96	0.5
97	0.5
98	0.5
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	397083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.49471710335413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.227015907091136	10.399027527674432
2	9.38378496900624	3.471008967209542
3	5.996604236469403	3.3271649680282955
4	4.2627361835808095	3.153523992062341
5	3.5638782394941586	3.295645991512212
6	3.3687402556313195	3.7382338813549283
7	2.5845725358282836	3.3460656518269905
8	2.5024858787415662	3.702621470597107
9	2.1052809332725233	3.504291775556462
>10	9.745195506030067	29.11412635288312
>50	0.15187655054272586	1.8138631524402709
>100	0.049155302402154474	1.9475133019094635
>500	0.015366869558286141	1.9127057419572375
>1k	0.03632169164949972	16.70769004680659
>5k	0.0055879525614614955	7.291431676784225
>10k+	0.0013969881403653739	3.2750855013967204
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	12676	3.192279699710136	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	8583	2.1615128323297648	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7629	1.9212607943427444	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6890	1.7351536076840357	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5119	1.289151134649431	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	4941	1.244324234479945	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	4711	1.1864018353845418	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4482	1.1287312728069445	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3954	0.9957615914053234	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	3708	0.9338098080250224	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3655	0.9204624725812991	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3618	0.9111445214224735	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	3078	0.7751528018071788	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	2855	0.7189932583364183	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC	2710	0.6824769632545337	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2512	0.6326133327289257	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2360	0.5943341820223984	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2249	0.5663803285459211	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA	2171	0.5467370801570453	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2042	0.5142501693600582	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1822	0.45884613544271596	No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT	1674	0.4215743308074131	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1619	0.4077233223280775	No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG	1594	0.401427409382925	No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC	1538	0.38732456438578333	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1498	0.3772511036735393	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	1356	0.3414903181450729	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT	1354	0.34098664510946075	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1117	0.2813013903894148	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT	1045	0.26316916110737554	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG	1003	0.2525920273595193	No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG	983	0.24755529700339726	No Hit
GGGGGGAGCGAAAGCAGGGTCCGGTCGGTCTGCAGGCGGTCAGCCTCCTC	733	0.18459616755187203	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	722	0.1818259658560049	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	704	0.1772929085354951	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	704	0.1772929085354951	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	649	0.16344190005615955	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	635	0.15991618880687414	No Hit
CGGGTTATTAGTAGAAACAAGGGCTCCACGGTGACGACCACCAATTTCTC	615	0.15487945845075213	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	577	0.14530967077412027	No Hit
GGGGGGAGCGAAAGCAGGCCCTGTCCCGTGGGGACTCCCTCCTCAAGCAG	553	0.13926559434677385	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	528	0.13296968140162133	No Hit
GGGGGGAGCGAAAGCAGGAAGTATTTCGAAGAAAATGGAAAGAAATCAAA	475	0.11962234595789796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	2.5183651780610097E-4	0.0	0.0
15-19	5.540403391734221E-4	0.0	0.0
20-24	0.0010073460712244039	0.0	0.0
25-29	0.0011584479819080646	0.0	0.0
30-34	0.003525711249285414	0.0	0.0
35-39	0.003525711249285414	0.0	0.0
40-44	0.003727180463530294	0.0	0.0
45-49	0.0052381995703669	0.0	0.0
50-54	0.0066484840700810665	0.0	0.0
55-59	0.009166849248142078	0.0	0.0
60-64	0.012088152854692847	0.0	0.0
65-69	0.015916067925345584	0.0	0.0
70-74	0.020902430977906384	0.0	0.0
75-79	0.02845752651208941	0.0	0.0
80-84	0.04120045431307812	0.0	0.0
85-89	0.05520256470309733	0.0	0.0
90-94	0.07318369207445294	0.0	0.0
95-99	0.09690669205178767	0.0	0.0
100-104	0.12798331834906054	0.0	0.0
105-109	0.16616173444846544	0.0	0.0
110-114	0.21542095733133876	0.0	0.0
115-119	0.274703273622895	0.0	0.0
120-124	0.34481456018011347	0.0	0.0
125-129	0.4455491673025539	0.0	0.0
130-134	0.558120090761881	0.0	0.0
135-139	0.680915576844136	0.0	0.0
140-144	0.8266785533503072	0.0	0.0
145-149	1.013742718776679	0.0	0.0
150-154	1.300131206825777	0.0	0.0
155-159	1.5520180919354392	0.0	0.0
160-164	1.8168493740603353	0.0	0.0
165-169	2.109886346179514	0.0	0.0
170-174	2.4400440210233123	0.0	0.0
175-179	2.819612020660668	0.0	0.0
180-184	3.2246155085964396	0.0	0.0
185-189	3.6963556737508276	0.0	0.0
190-194	4.24379789615773	0.0	0.0
195-199	4.736692328807831	0.0	0.0
200-204	5.238652876098952	0.0	0.0
205-209	5.736684773712296	0.0	0.0
210-214	6.258540405910099	0.0	0.0
215-219	6.76095425893327	0.0	0.0
220-224	7.286889642719532	0.0	0.0
225-229	7.755053729321074	0.0	0.0
230-234	8.179347894520792	0.0	0.0
235-239	8.571457352744893	0.0	0.0
240-244	8.915264566853782	0.0	0.0
245-249	9.181052827746342	0.0	0.0
250-254	9.416419237287922	0.0	0.0
255-259	9.609980784873692	0.0	0.0
260-264	9.76929256603783	0.0	0.0
265-269	9.902765920475066	0.0	0.0
270-274	10.022539368343647	0.0	0.0
275-279	10.087412455330497	0.0	0.0
280-284	10.128159603911524	0.0	0.0
285-289	10.163718920225747	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6920	0.0	288.81863	1
GGGTTAT	6935	0.0	288.40662	2
GTTATTA	6975	0.0	285.69534	4
TTAGTAG	7100	0.0	281.70422	8
GGTTATT	7095	0.0	281.27908	3
TTATTAG	7115	0.0	280.0738	5
TAGTAGA	7155	0.0	278.71417	9
TATTAGT	7150	0.0	278.2902	6
GGGGGGA	7615	0.0	275.63034	1
ATTAGTA	7240	0.0	274.62708	7
GGGGAGC	7805	0.0	270.05444	3
GGGAGCG	5860	0.0	267.06058	4
GGAGCGA	5855	0.0	265.7771	5
GCGAAAG	5995	0.0	262.76898	8
CGAAAGC	6055	0.0	260.65237	9
AGCGAAA	5955	0.0	260.07556	7
GAGCGAA	6105	0.0	253.92712	6
GGGGGAG	8515	0.0	248.74925	2
GGGAGCA	2245	0.0	236.5256	4
CAAAAGC	2645	0.0	192.39131	9
>>END_MODULE