FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6929_flu.3520000003bb82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6929_flu.3520000003bb82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397083
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC126763.192279699710136No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT85832.1615128323297648No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT76291.9212607943427444No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG68901.7351536076840357No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA51191.289151134649431No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA49411.244324234479945No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG47111.1864018353845418No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA44821.1287312728069445No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT39540.9957615914053234No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC37080.9338098080250224No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT36550.9204624725812991No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36180.9111445214224735No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG30780.7751528018071788No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC28550.7189932583364183No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC27100.6824769632545337No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT25120.6326133327289257No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA23600.5943341820223984No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA22490.5663803285459211No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA21710.5467370801570453No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20420.5142501693600582No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG18220.45884613544271596No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT16740.4215743308074131No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA16190.4077233223280775No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG15940.401427409382925No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC15380.38732456438578333No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14980.3772511036735393No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG13560.3414903181450729No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT13540.34098664510946075No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11170.2813013903894148No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT10450.26316916110737554Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG10030.2525920273595193No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG9830.24755529700339726No Hit
GGGGGGAGCGAAAGCAGGGTCCGGTCGGTCTGCAGGCGGTCAGCCTCCTC7330.18459616755187203No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7220.1818259658560049No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT7040.1772929085354951No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7040.1772929085354951No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6490.16344190005615955No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6350.15991618880687414No Hit
CGGGTTATTAGTAGAAACAAGGGCTCCACGGTGACGACCACCAATTTCTC6150.15487945845075213No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5770.14530967077412027No Hit
GGGGGGAGCGAAAGCAGGCCCTGTCCCGTGGGGACTCCCTCCTCAAGCAG5530.13926559434677385No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG5280.13296968140162133No Hit
GGGGGGAGCGAAAGCAGGAAGTATTTCGAAGAAAATGGAAAGAAATCAAA4750.11962234595789796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA69200.0288.818631
GGGTTAT69350.0288.406622
GTTATTA69750.0285.695344
TTAGTAG71000.0281.704228
GGTTATT70950.0281.279083
TTATTAG71150.0280.07385
TAGTAGA71550.0278.714179
TATTAGT71500.0278.29026
GGGGGGA76150.0275.630341
ATTAGTA72400.0274.627087
GGGGAGC78050.0270.054443
GGGAGCG58600.0267.060584
GGAGCGA58550.0265.77715
GCGAAAG59950.0262.768988
CGAAAGC60550.0260.652379
AGCGAAA59550.0260.075567
GAGCGAA61050.0253.927126
GGGGGAG85150.0248.749252
GGGAGCA22450.0236.52564
CAAAAGC26450.0192.391319
Produced by FastQC (version 0.11.2)