##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6777_flu.3520000003b9fe.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294389
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12478047753143	34.0	34.0	34.0	34.0	34.0
2	33.439391417478234	34.0	34.0	34.0	34.0	34.0
3	33.41358202921984	34.0	34.0	34.0	34.0	34.0
4	33.44634480228541	34.0	34.0	34.0	34.0	34.0
5	33.444099473825446	34.0	34.0	34.0	34.0	34.0
6	36.99673900859067	38.0	38.0	38.0	36.0	38.0
7	36.14456382541467	38.0	38.0	38.0	34.0	38.0
8	36.904269520939984	38.0	38.0	38.0	36.0	38.0
9	36.968140793304094	38.0	38.0	38.0	36.0	38.0
10-14	36.9845102908057	38.0	38.0	38.0	36.6	38.0
15-19	36.89263049910153	38.0	38.0	38.0	36.6	38.0
20-24	36.99565269082744	38.0	38.0	38.0	36.8	38.0
25-29	36.92273488479529	38.0	38.0	38.0	36.0	38.0
30-34	36.59406024002256	38.0	38.0	38.0	34.6	38.0
35-39	36.87523854491846	38.0	38.0	38.0	36.0	38.0
40-44	36.393444048520834	38.0	37.6	38.0	34.0	38.0
45-49	36.73148113550438	38.0	38.0	38.0	35.2	38.0
50-54	36.695149614965224	38.0	38.0	38.0	35.2	38.0
55-59	36.35913162516262	38.0	37.4	38.0	33.8	38.0
60-64	36.54778745129743	38.0	37.6	38.0	34.2	38.0
65-69	36.415209128058464	38.0	37.4	38.0	34.0	38.0
70-74	36.29417267628885	38.0	37.0	38.0	34.0	38.0
75-79	36.335118499672205	38.0	37.0	38.0	34.0	38.0
80-84	35.682967094558556	38.0	37.0	38.0	30.6	38.0
85-89	35.904701602301714	38.0	37.0	38.0	32.0	38.0
90-94	35.90283400534666	38.0	37.0	38.0	32.0	38.0
95-99	35.00034240409798	38.0	36.2	38.0	27.0	38.0
100-104	35.36649195452276	38.0	36.2	38.0	28.8	38.0
105-109	35.21155070332111	38.0	35.8	38.0	27.8	38.0
110-114	34.73934080417407	38.0	35.2	38.0	24.2	38.0
115-119	34.79882604309264	38.0	35.2	38.0	25.6	38.0
120-124	34.33936662035606	37.6	34.6	38.0	24.4	38.0
125-129	33.8893695076922	37.0	34.0	38.0	23.0	38.0
130-134	33.7749637384549	37.0	34.0	38.0	23.0	38.0
135-139	33.15078144903512	37.0	31.6	38.0	21.6	38.0
140-144	31.85179337543183	36.2	28.4	38.0	17.4	38.0
145-149	31.778670398690174	35.4	28.2	37.6	18.4	38.0
150-154	30.71469178535883	35.0	26.4	37.0	14.2	38.0
155-159	30.57286515460836	35.0	26.0	37.0	15.0	37.6
160-164	29.881726559076593	35.0	24.4	37.0	11.0	37.4
165-169	28.757860517886193	33.4	23.0	36.6	9.8	37.0
170-174	28.09889160260743	32.6	21.8	36.2	9.4	37.0
175-179	27.24393302738893	30.4	20.2	36.0	9.2	37.0
180-184	26.33851944196285	28.6	20.0	35.0	9.0	37.0
185-189	25.606447931138728	27.8	19.0	35.0	9.0	36.6
190-194	24.48884231408103	25.8	16.0	34.6	8.6	36.0
195-199	23.600820003464804	24.8	15.0	33.6	8.0	36.0
200-204	22.690010156629494	23.4	14.4	32.2	8.0	36.0
205-209	21.78122076572155	22.6	10.4	31.2	8.0	35.4
210-214	20.47604292279943	20.4	9.0	28.8	8.0	35.0
215-219	19.857190316214265	20.0	9.0	28.0	8.0	34.2
220-224	19.05296121798029	18.6	9.0	26.2	8.0	33.4
225-229	18.215883066283048	16.8	9.0	25.4	8.0	32.4
230-234	17.281139580622916	15.6	9.0	24.6	7.6	31.4
235-239	16.32237345824742	14.4	8.0	22.8	7.0	29.4
240-244	15.423450604472313	13.2	8.0	21.2	7.0	28.2
245-249	14.72699115795767	12.6	8.0	20.2	7.0	26.8
250-254	14.007892957956988	11.6	8.0	18.8	7.0	25.4
255-259	13.424089894663183	11.0	8.0	17.6	7.0	24.4
260-264	12.829043204739307	9.8	7.8	16.4	7.0	23.2
265-269	12.418807767953286	9.0	7.0	15.4	7.0	22.0
270-274	11.948304454310453	8.6	7.0	14.0	7.0	21.0
275-279	11.471574005822228	8.0	7.0	13.4	7.0	20.2
280-284	11.339360505997167	8.0	7.0	13.0	7.0	19.6
285-289	11.072207861027415	8.0	7.0	13.0	7.0	19.0
290-294	10.819287405439741	8.0	7.0	13.0	7.0	18.8
295-299	10.54792808155196	8.0	7.0	13.0	7.0	18.0
300-301	10.221788178226769	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.14979526909561258
1101	2	0.06970017502841586
1101	3	0.13785817661915445
1101	4	0.11570494174597457
1101	5	0.15015940441095665
1101	6	0.20161619928975938
1101	7	0.9947813774579757
1101	8	0.3343063549648235
1101	9	0.2575878009759762
1101	10-14	0.14032504653965105
1101	15-19	0.09738410466366787
1101	20-24	0.1361220482631964
1101	25-29	0.03037945095116612
1101	30-34	0.41131029590719237
1101	35-39	0.24508294078044202
1101	40-44	0.4979159010224805
1101	45-49	0.23089542849883316
1101	50-54	0.17424424146447848
1101	55-59	0.26574232735755743
1101	60-64	0.24694123526288791
1101	65-69	0.2863343224870434
1101	70-74	0.19470537380799868
1101	75-79	0.3133397652744705
1101	80-84	0.5472631818827978
1101	85-89	0.34002107813766713
1101	90-94	0.3297532809081716
1101	95-99	0.9411998064980907
1101	100-104	0.3458692311838405
1101	105-109	0.49999923903575905
1101	110-114	0.2506452929774383
1101	115-119	0.30625060035001184
1101	120-124	0.8880240983927834
1101	125-129	0.4458896576118221
1101	130-134	0.7494491925628779
1101	135-139	0.6798161467246402
1101	140-144	1.1811169668013015
1101	145-149	1.4243140043224365
1101	150-154	0.791671261471123
1101	155-159	0.6414980522364289
1101	160-164	0.9113179905866744
1101	165-169	0.4838253020605414
1101	170-174	0.4260428503846896
1101	175-179	0.8118851614884974
1101	180-184	0.7706913835327036
1101	185-189	0.9430593743285058
1101	190-194	1.052250340659569
1101	195-199	1.243681895816664
1101	200-204	1.162034361740531
1101	205-209	0.7350934437453596
1101	210-214	1.3411270342359565
1101	215-219	0.910737954416458
1101	220-224	0.7484556896800001
1101	225-229	0.8000692450445612
1101	230-234	0.45640351085942754
1101	235-239	0.23621722253753674
1101	240-244	0.23122795969523935
1101	245-249	-0.0013590337773212724
1101	250-254	0.08890436979151595
1101	255-259	0.13076891872321106
1101	260-264	0.19973221065477276
1101	265-269	0.17808132874876392
1101	270-274	-0.053115436852065656
1101	275-279	0.016990411306194275
1101	280-284	0.048203395320573605
1101	285-289	-0.008196239551798712
1101	290-294	-0.11704286072558467
1101	295-299	0.08998785279259991
1101	300-301	-0.20284830183087976
1102	1	-0.05425206047740261
1102	2	-0.008363059513406768
1102	3	0.09349500652102449
1102	4	0.10133466291074456
1102	5	0.08560609220452164
1102	6	-0.1642246580106459
1102	7	0.7984971874221642
1102	8	0.23617920822587024
1102	9	0.09054576641555201
1102	10-14	0.09196503883298845
1102	15-19	0.14024314571496177
1102	20-24	0.12555926216975166
1102	25-29	0.1098823708483252
1102	30-34	0.4846385800440771
1102	35-39	0.12169320260785099
1102	40-44	0.5857679329918639
1102	45-49	0.17095017241820898
1102	50-54	0.26747433201783366
1102	55-59	0.1588010109788911
1102	60-64	0.11348765132996164
1102	65-69	0.0673028380897307
1102	70-74	0.18785640457487318
1102	75-79	0.23074819876127606
1102	80-84	0.8181047367695626
1102	85-89	0.3907761323168657
1102	90-94	0.35612204498446687
1102	95-99	0.9661707404001234
1102	100-104	0.5939156572164279
1102	105-109	0.7912588142389012
1102	110-114	0.8401967781719932
1102	115-119	0.5430346608340741
1102	120-124	0.9841408677042978
1102	125-129	0.9797744163466007
1102	130-134	1.0130637528509396
1102	135-139	1.1324586702598864
1102	140-144	1.6857050918884973
1102	145-149	1.940600320335701
1102	150-154	1.7158934526894996
1102	155-159	1.220502696168424
1102	160-164	1.6245282829750103
1102	165-169	1.50565643301411
1102	170-174	1.5303745294418185
1102	175-179	1.4765221840570746
1102	180-184	1.5515914692361648
1102	185-189	1.6913981222606616
1102	190-194	1.9190651613798728
1102	195-199	1.7536600381109935
1102	200-204	1.6796653584917678
1102	205-209	1.201442542530227
1102	210-214	1.7712454910420696
1102	215-219	1.7952601742026637
1102	220-224	1.4849308190656636
1102	225-229	1.225062103089062
1102	230-234	0.9684112972518442
1102	235-239	1.063125453392118
1102	240-244	1.0910509720724146
1102	245-249	0.44579979666933234
1102	250-254	0.8866069854081466
1102	255-259	0.7765657948650926
1102	260-264	0.5887063600977012
1102	265-269	0.6844380013283047
1102	270-274	0.47825535354648885
1102	275-279	0.4589007349198244
1102	280-284	0.15533671403781213
1102	285-289	0.549644298851991
1102	290-294	0.19202908368010796
1102	295-299	0.04444941578464956
1102	300-301	-0.0036840767164427746
1103	1	-0.2450259801610173
1103	2	-0.05222762273513126
1103	3	0.12464319801854629
1103	4	0.21231906047118088
1103	5	0.1408782095546144
1103	6	0.09122652559395306
1103	7	1.0348796489329217
1103	8	0.34417249907681935
1103	9	0.3720282482671706
1103	10-14	0.10220682829508121
1103	15-19	0.16981642116020623
1103	20-24	0.14273294976748474
1103	25-29	0.05293996882624441
1103	30-34	0.4335266686368584
1103	35-39	0.1214207684738966
1103	40-44	0.4144602158031745
1103	45-49	0.09858673127831707
1103	50-54	0.0781039316363561
1103	55-59	0.16686932959011358
1103	60-64	0.15498233769724834
1103	65-69	0.23100007273320955
1103	70-74	0.13442830284389373
1103	75-79	0.28929152645918776
1103	80-84	0.8170456782512403
1103	85-89	0.46041923359768333
1103	90-94	0.41893561934031
1103	95-99	1.0247364096977662
1103	100-104	0.559318189486703
1103	105-109	0.7192274959844767
1103	110-114	0.5359191954663842
1103	115-119	0.48244024089868986
1103	120-124	0.9815155630082302
1103	125-129	0.6577202563073783
1103	130-134	0.8734457608605766
1103	135-139	0.7780008542012737
1103	140-144	1.4124926699590539
1103	145-149	1.4708418430324421
1103	150-154	1.7997084642437784
1103	155-159	0.7848028205477782
1103	160-164	0.751647317017607
1103	165-169	1.3954579929246087
1103	170-174	1.481467767273152
1103	175-179	1.3191863031144635
1103	180-184	1.0129588418306916
1103	185-189	1.0053379466623618
1103	190-194	1.4550434733553068
1103	195-199	1.4770873728915284
1103	200-204	1.1891682078793764
1103	205-209	1.0789548887650042
1103	210-214	1.7001571883700812
1103	215-219	1.6584922367324673
1103	220-224	1.3835399711185907
1103	225-229	0.7243114920296385
1103	230-234	1.720438880791086
1103	235-239	1.0047635261457017
1103	240-244	0.7749363429929854
1103	245-249	0.6166340053265333
1103	250-254	0.8452466820959224
1103	255-259	0.731320720854379
1103	260-264	0.23880389618605058
1103	265-269	0.6428966310212925
1103	270-274	0.3926246701936478
1103	275-279	0.29245081511616355
1103	280-284	0.28670865498929743
1103	285-289	0.2967027104642863
1103	290-294	0.048081489737015204
1103	295-299	0.04925686120612127
1103	300-301	-0.07502714031663871
2116	1	0.07384024980643744
2116	2	-0.00563923234251007
2116	3	0.028257913958064762
2116	4	0.17898984694173947
2116	5	0.12997061675602595
2116	6	0.18493393712116557
2116	7	1.137304565506895
2116	8	0.2667696234536976
2116	9	0.3417493375433267
2116	10-14	0.1610939868663408
2116	15-19	0.3103989614312894
2116	20-24	0.16112836854555468
2116	25-29	0.17064342534170862
2116	30-34	0.512449916690251
2116	35-39	-0.04399331301582521
2116	40-44	0.40621533465951387
2116	45-49	0.18531486541913011
2116	50-54	0.25095112810980424
2116	55-59	0.40501409949906275
2116	60-64	0.3919554353777954
2116	65-69	0.47214340071079874
2116	70-74	0.30973057285584105
2116	75-79	0.39672579498800076
2116	80-84	0.8529993716554358
2116	85-89	0.43145734178371953
2116	90-94	0.617119939366674
2116	95-99	1.2037807799857418
2116	100-104	0.6457556302645102
2116	105-109	0.5726929926268411
2116	110-114	0.7351459906709437
2116	115-119	0.8269049547783069
2116	120-124	0.8766873176327081
2116	125-129	0.9537163012956356
2116	130-134	0.9735276625620628
2116	135-139	1.2425107211316089
2116	140-144	1.8141659695099897
2116	145-149	1.915386810691981
2116	150-154	2.082348434581192
2116	155-159	1.8872976089958406
2116	160-164	1.7969766953392003
2116	165-169	1.5087252446034292
2116	170-174	1.3606556715289102
2116	175-179	2.15183558420868
2116	180-184	2.0298955204447964
2116	185-189	1.697814360785042
2116	190-194	1.5867265780395208
2116	195-199	0.9184867878793739
2116	200-204	0.7927026700389774
2116	205-209	0.930051992542861
2116	210-214	1.543590138561651
2116	215-219	2.0037304849918556
2116	220-224	1.3553292019512035
2116	225-229	0.7163266529079806
2116	230-234	0.6839891629976549
2116	235-239	0.684599722742746
2116	240-244	0.5652323921010485
2116	245-249	0.9553609859222583
2116	250-254	0.6607651594164032
2116	255-259	1.2840388850697604
2116	260-264	0.6768609966680685
2116	265-269	0.4699756076620041
2116	270-274	0.6005445812058845
2116	275-279	0.5551153394848036
2116	280-284	0.24048115941808668
2116	285-289	0.21231759119596383
2116	290-294	0.12434037821392074
2116	295-299	0.16946482890685743
2116	300-301	0.1478715537055244
1117	1	0.1499742321511235
1117	2	0.18739929583173165
1117	3	0.0946402256485257
1117	4	0.1671096390381024
1117	5	0.10707811613726648
1117	6	0.12585300320482418
1117	7	1.0376576716862544
1117	8	0.14884205972133202
1117	9	0.1780598729776912
1117	10-14	0.16215528543906288
1117	15-19	0.08291608048087085
1117	20-24	0.20837496536099565
1117	25-29	0.12810304088498725
1117	30-34	0.3711490339248016
1117	35-39	0.13241886419727678
1117	40-44	0.40934675945944576
1117	45-49	0.252808966565901
1117	50-54	0.23941412621226021
1117	55-59	0.23041827407211457
1117	60-64	0.2696968758530076
1117	65-69	0.3637823743928408
1117	70-74	0.18200810771269715
1117	75-79	0.46836146861229366
1117	80-84	0.7406812460882364
1117	85-89	0.32940173581062027
1117	90-94	0.34514930988066794
1117	95-99	0.9117184088942523
1117	100-104	0.38121541081068244
1117	105-109	0.49864497678655084
1117	110-114	0.6280868371357542
1117	115-119	0.5817944853450996
1117	120-124	0.8742198644522787
1117	125-129	0.878308001650403
1117	130-134	0.9865153698469342
1117	135-139	0.9106407434069936
1117	140-144	1.1604301391329592
1117	145-149	1.6856631905286186
1117	150-154	2.1461352508504703
1117	155-159	1.571349089896767
1117	160-164	2.029609316410067
1117	165-169	1.743600914927658
1117	170-174	1.2435155015759385
1117	175-179	1.3671567623292944
1117	180-184	1.352525006460798
1117	185-189	1.0073630778875966
1117	190-194	-1.3759900295011107
1117	195-199	0.9232326009311045
1117	200-204	1.402521661871333
1117	205-209	1.3216978963461763
1117	210-214	1.9732167370271299
1117	215-219	1.6153272179346772
1117	220-224	1.6889006894522325
1117	225-229	1.0252724494594183
1117	230-234	1.1087303559685324
1117	235-239	0.7236370958967733
1117	240-244	0.913024488462737
1117	245-249	1.0213835913178535
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2108	235-239	-0.6287461286454761
2108	240-244	-0.44929689693762853
2108	245-249	-0.8642089065508607
2108	250-254	-0.792558544643942
2108	255-259	-0.5714603927347657
2108	260-264	-0.5395200015665633
2108	265-269	-0.2663245046795133
2108	270-274	-0.39227840065898256
2108	275-279	-0.34434413732469693
2108	280-284	-0.6253012190123428
2108	285-289	-1.0256281638995262
2108	290-294	-0.6659908687769303
2108	295-299	-0.5946462287082905
2108	300-301	2.486201677281194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	167.0
11	623.0
12	623.0
13	581.0
14	530.0
15	851.0
16	1262.0
17	1896.0
18	2350.0
19	2854.0
20	3761.0
21	5087.0
22	7343.0
23	10499.0
24	15196.0
25	22072.0
26	31069.0
27	41613.0
28	49032.0
29	46168.0
30	32378.0
31	14518.0
32	3512.0
33	385.0
34	12.0
35	4.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.775531694458696	22.857171973137582	14.359911545608023	34.007384786795704
2	44.218024450641835	24.2339217837623	17.387198570598766	14.160855194997096
3	43.22579987703345	23.260379973436507	19.177686666281687	14.336133483248355
4	43.62900787733237	22.166589104891827	19.313221621731792	14.89118139604401
5	27.578136411346893	22.31910839059883	35.637540804853444	14.465214393200835
6	26.74386610912772	21.6278461491428	35.910309148779334	15.717978592950143
7	16.7696483224577	47.31732503592186	20.26230599648764	15.650720645132802
8	26.01014304202942	20.896840574885612	36.93106739721932	16.16194898586564
9	15.562402127796895	19.462683727992555	38.11045929025881	26.86445485395174
10-14	26.025428939260635	33.96696208078427	23.844980620879177	16.162628359075917
15-19	21.14854834929294	35.2507736362432	22.05204678163926	21.548631232824597
20-24	25.145844443916044	30.718199389243484	26.581156225266568	17.554799941573904
25-29	19.126801612832	25.63370234621538	37.84727010859781	17.392225932354812
30-34	19.603381919840754	28.54563180010123	31.13676122409465	20.714225055963368
35-39	20.69071874288781	29.979177211105036	28.10471858663197	21.225385459375183
40-44	20.395734894985885	28.903729419237813	29.05427852263502	21.646257163141286
45-49	20.81239448484828	30.601890695644197	26.437332916651098	22.148381902856425
50-54	22.03017096426837	29.473723542659542	27.87950636742541	20.61659912564668
55-59	21.317100842762468	26.98959539928462	29.622981836957223	22.07032192099569
60-64	21.98764220130508	26.15885783775888	28.370693198455104	23.482806762480934
65-69	20.270730224295068	26.932867736226555	29.58330644147709	23.213095598001285
70-74	20.928159679879343	26.093638009572366	29.644721779686062	23.33348053086223
75-79	20.457965481047186	25.805244081810123	30.646049954312147	23.09074048283054
80-84	22.9742959145892	25.761016885821142	27.68377894554484	23.580908254044818
85-89	20.845072336262565	28.090587623858227	26.544470071911654	24.51986996796755
90-94	20.374130826899105	30.108733682304706	28.823359568462138	20.693775922334055
95-99	21.940018139264712	29.964298937799988	26.795838159713846	21.29984476322145
100-104	22.572582535352883	28.653516265893085	26.557038476301763	22.216862722452266
105-109	23.616235660979182	27.5154302640384	24.044852219342435	24.823481855639987
110-114	21.40073168494747	27.75484138333973	29.455584277945167	21.388842653767632
115-119	22.99916097408531	28.051863350872488	27.203190336595455	21.745785338446748
120-124	18.436694305833438	29.14803202565313	27.960012092843144	24.45526157567029
125-129	20.70539320422978	25.39463091351919	30.398214607203393	23.50176127504764
130-134	24.73747320721902	28.731847997037935	26.126723484912816	20.403955310830227
135-139	24.00979656169218	27.47480374606388	25.086195476053792	23.429204216190143
140-144	21.143588924857927	29.129009575765398	26.668727432071172	23.058674067305503
145-149	21.133941825272004	27.036404213472647	28.71622241320158	23.113431548053764
150-154	23.154533627275477	25.650754613793314	25.642466260627945	25.552245498303268
155-159	22.068215864043832	26.995030384966828	30.059886748485848	20.87686700250349
160-164	25.40291926668456	28.273678704027667	26.373811521490275	19.949590507797506
165-169	20.786782114820866	25.433083437220823	28.150576278325616	25.6295581696327
170-174	22.931359527699744	28.59257648893131	26.486791286359207	21.98927269700974
175-179	21.44006739382246	28.97146292830235	27.983654280560756	21.604815397314436
180-184	20.914708090315877	28.91745275808539	26.64848211040494	23.519357041193796
185-189	23.399991168148265	27.31977077947885	27.508908281219746	21.771329771153134
190-194	22.134930313292955	27.865171592688586	26.215653438137977	23.784244655880485
195-199	23.003305150667995	29.167733848751144	26.765945738461696	21.06301526211917
200-204	24.30450866031	27.535403836420517	26.83836692267714	21.321720580592345
205-209	23.791853635835576	27.23077288893267	27.1698331119709	21.807540363260856
210-214	25.354412019470836	27.056785409780936	26.78238657015038	20.80641600059785
215-219	25.341435991154558	27.348372391631482	26.48835384474284	20.82183777247112
220-224	23.05045365146116	27.27112765762308	26.854468067760685	22.823950623155078
225-229	24.98381393326517	28.255811188597402	25.273498670126944	21.48687620801049
230-234	24.66742982923954	27.161408884163468	26.657993335348806	21.513167951248178
235-239	25.16907900770749	27.48947820740585	24.528294195774976	22.813148589111684
240-244	26.340318422223657	30.681309423925484	22.145052974126074	20.833319179724786
245-249	25.009222491329496	29.397973429713748	22.875039488567847	22.717764590388906
250-254	25.38634256035382	27.918230640411156	23.767260325623578	22.928166473611448
255-259	25.49252859311999	28.64950796395246	24.733329030636337	21.124634412291222
260-264	25.61868819826828	29.311217470761473	24.06815472045491	21.00193961051534
265-269	24.85242315439775	29.145110720848944	24.173525505368747	21.828940619384557
270-274	24.49738271470741	30.67601031288533	22.447306115377952	22.379300857029307
275-279	25.20759946873015	30.059139437954542	21.931525974136264	22.801735119179046
280-284	23.2608555346837	30.653319247662107	24.231204290921195	21.854620926732995
285-289	24.5755785712102	30.318048568390804	23.987852807000262	21.118520053398733
290-294	24.231543977526336	31.58786503571805	22.36829501102283	21.81229597573279
295-299	23.649728760245797	32.82051978844318	20.529299668126185	23.000451783184833
300-301	20.356229342808323	35.782247298642275	26.022371759814394	17.839151598735008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.25
27	0.5
28	1.0
29	0.75
30	0.75
31	1.5
32	3.5
33	9.25
34	24.5
35	73.25
36	260.0
37	798.0
38	1689.75
39	2837.75
40	3852.75
41	5121.25
42	7440.75
43	10347.25
44	11938.0
45	11634.5
46	10145.25
47	8280.0
48	6520.5
49	5252.25
50	4054.75
51	3085.0
52	2169.25
53	1138.5
54	514.75
55	219.75
56	130.0
57	84.75
58	64.0
59	57.75
60	51.0
61	45.75
62	39.5
63	36.25
64	28.75
65	22.75
66	16.0
67	11.25
68	10.25
69	7.75
70	5.5
71	5.5
72	4.25
73	3.0
74	2.75
75	2.0
76	1.5
77	1.25
78	0.75
79	0.75
80	1.5
81	1.5
82	1.0
83	0.5
84	1.5
85	0.5
86	1.0
87	1.0
88	0.0
89	0.25
90	0.5
91	0.5
92	0.5
93	0.75
94	0.0
95	0.0
96	0.25
97	0.25
98	0.0
99	0.25
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	294389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.62420916279628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.20817863109805	8.672552327147145
2	7.773750485398068	2.7401240906808932
3	6.151401029797658	3.252407351801831
4	5.6789562093044115	4.003484482365666
5	4.974185450072579	4.383304239330855
6	3.913622681900872	4.1384702838050815
7	3.616132548026562	4.461203347077092
8	3.025292204533331	4.2654706073018
9	2.546891009652652	4.039824588206737
>10	12.963195708039816	35.71857535037374
>50	0.07335947268357207	0.8429294098705874
>100	0.027687974973226524	0.9317861290953735
>500	0.017754972944790007	2.2516142255703624
>1k	0.021700522487914913	10.514370876328295
>5k	0.007891099086514513	9.78388269104455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9609	3.264048588771999	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7915	2.6886194796680583	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5615	1.9073402878504293	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5001	1.6987727122956362	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4872	1.6549531402328213	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4804	1.6318544510834303	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3463	1.176334713593239	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3184	1.081562150759709	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2892	0.9823736620593839	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2227	0.7564820696425478	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2085	0.7082465717129376	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1872	0.6358933248185223	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1833	0.6226455472181366	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1525	0.5180220728356019	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1484	0.5040949220249398	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	965	0.3277975739582661	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	912	0.3097941838859468	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	855	0.29043204739307515	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	756	0.2568030734844033	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	656	0.2228344129705933	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	649	0.22045660673462664	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT	602	0.20449133629313596	Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	562	0.19090387208761198	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	519	0.1762973480666737	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	414	0.14063025452717323	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	312	0.10598222080308707	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	6.793732102761992E-4	0.0	0.0
9	6.793732102761992E-4	0.0	0.0
10-14	0.001698433025690498	0.0	0.0
15-19	0.0023778062359666972	0.0	0.0
20-24	0.0029892421252152765	0.0	0.0
25-29	0.004347988545767675	0.0	0.0
30-34	0.006114358892485792	0.0	0.0
35-39	0.006861669423789611	0.0	0.0
40-44	0.008628039770507728	0.0	0.0
45-49	0.011277595290584905	0.0	0.0
50-54	0.014878273305048761	0.0	0.0
55-59	0.021468193444727897	0.0	0.0
60-64	0.03145497963578802	0.0	0.0
65-69	0.04612944097775392	0.0	0.0
70-74	0.0692281301271447	0.0	0.0
75-79	0.10428378777739655	0.0	0.0
80-84	0.15014147947104	0.0	0.0
85-89	0.21434224784214084	0.0	0.0
90-94	0.2932174775552076	0.0	0.0
95-99	0.3932212141078641	0.0	0.0
100-104	0.5318812863252363	0.0	0.0
105-109	0.6994826573003746	0.0	0.0
110-114	0.901052688789323	0.0	0.0
115-119	1.14433623538923	0.0	0.0
120-124	1.4463176273570002	0.0	0.0
125-129	1.800474881873983	0.0	0.0
130-134	2.2116315487331386	0.0	0.0
135-139	2.6500310813243706	0.0	0.0
140-144	3.1310952515209465	0.0	0.0
145-149	3.6994588792380148	0.0	0.0
150-154	4.368030055470823	0.0	0.0
155-159	5.114049777675117	0.0	0.0
160-164	5.909595806908547	0.0	0.0
165-169	6.782658319434489	0.0	0.0
170-174	7.749338460336494	0.0	0.0
175-179	8.785790229933863	0.0	0.0
180-184	9.890043445916797	0.0	0.0
185-189	11.059108866160079	0.0	0.0
190-194	12.269072553661989	0.0	0.0
195-199	13.403489940181188	0.0	0.0
200-204	14.462836586964865	0.0	0.0
205-209	15.495008305337496	0.0	0.0
210-214	16.55496638801042	0.0	0.0
215-219	17.671516259099356	0.0	0.0
220-224	18.688470017561798	0.0	0.0
225-229	19.58028323069136	0.0	0.0
230-234	20.374470513504242	0.0	0.0
235-239	21.067499125306988	0.0	0.0
240-244	21.6453060406469	0.0	0.0
245-249	22.109114131302462	0.0	0.0
250-254	22.523735601534025	0.0	2.7174928411047967E-4
255-259	22.841274640017122	0.0	3.396866051380996E-4
260-264	23.09311828906651	0.0	3.396866051380996E-4
265-269	23.297541688038617	0.0	4.076239261657195E-4
270-274	23.44795491679377	0.0	6.793732102761992E-4
275-279	23.54836627727259	0.0	6.793732102761992E-4
280-284	23.612567045643686	0.0	6.793732102761992E-4
285-289	23.665082594798037	0.0	6.793732102761992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4800	0.0	291.0052	1
GGGTTAT	4810	0.0	290.09354	2
GTTATTA	4840	0.0	287.07645	4
GGTTATT	4945	0.0	282.77048	3
TTATTAG	4980	0.0	278.11746	5
TTAGTAG	4970	0.0	278.0835	8
TATTAGT	5025	0.0	274.74625	6
GGGGAGC	3270	0.0	270.19113	3
GGGAGCG	2375	0.0	264.56842	4
TAGTAGA	5225	0.0	264.22968	9
GGAGCGA	2400	0.0	261.8125	5
ATTAGTA	5285	0.0	260.9508	7
GCGAAAG	2550	0.0	250.46077	8
CGAAAGC	2560	0.0	248.33008	9
GGGGGGA	3595	0.0	248.22672	1
GAGCGAA	2580	0.0	243.54651	6
AGCGAAA	2605	0.0	242.34166	7
GGGGGAG	4065	0.0	220.97787	2
GGGAGCA	1205	0.0	190.95436	4
GCGCGAA	35	1.0053172E-6	168.57143	6
>>END_MODULE