##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6667_flu.3520000003ba33.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348094
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.14086424931197	34.0	34.0	34.0	34.0	34.0
2	33.45656920257172	34.0	34.0	34.0	34.0	34.0
3	33.42846759783277	34.0	34.0	34.0	34.0	34.0
4	33.463877573299165	34.0	34.0	34.0	34.0	34.0
5	33.45796824995547	34.0	34.0	34.0	34.0	34.0
6	37.000080438042595	38.0	38.0	38.0	36.0	38.0
7	36.156512321384454	38.0	38.0	38.0	34.0	38.0
8	36.92044390308365	38.0	38.0	38.0	36.0	38.0
9	36.98751773946118	38.0	38.0	38.0	36.0	38.0
10-14	36.987882583440104	38.0	38.0	38.0	36.4	38.0
15-19	36.897146173160124	38.0	38.0	38.0	36.6	38.0
20-24	36.996307893844765	38.0	38.0	38.0	36.6	38.0
25-29	36.929108229386316	38.0	38.0	38.0	36.0	38.0
30-34	36.5881336650445	38.0	38.0	38.0	34.6	38.0
35-39	36.844686780007706	38.0	38.0	38.0	35.8	38.0
40-44	36.39881066608445	38.0	37.4	38.0	34.0	38.0
45-49	36.717207995541436	38.0	38.0	38.0	35.2	38.0
50-54	36.67918780559275	38.0	38.0	38.0	35.2	38.0
55-59	36.33899866128115	38.0	37.4	38.0	33.8	38.0
60-64	36.516394422196306	38.0	37.4	38.0	34.0	38.0
65-69	36.37177773819715	38.0	37.2	38.0	34.0	38.0
70-74	36.25784931656391	38.0	37.0	38.0	33.8	38.0
75-79	36.30020626612352	38.0	37.0	38.0	34.0	38.0
80-84	35.624214149051696	38.0	37.0	38.0	30.0	38.0
85-89	35.836590116462794	38.0	37.0	38.0	31.8	38.0
90-94	35.82521502812459	38.0	37.0	38.0	31.8	38.0
95-99	34.89929271978259	38.0	36.0	38.0	26.4	38.0
100-104	35.254378415025826	38.0	36.0	38.0	28.0	38.0
105-109	35.08545220543876	38.0	35.8	38.0	27.4	38.0
110-114	34.60383861830425	38.0	35.2	38.0	24.0	38.0
115-119	34.658600263147314	38.0	35.0	38.0	24.4	38.0
120-124	34.195354128482535	37.6	34.6	38.0	23.4	38.0
125-129	33.752532361948205	37.0	34.0	38.0	23.0	38.0
130-134	33.567672525237434	37.0	33.6	38.0	22.8	38.0
135-139	32.895356426712326	37.0	31.0	38.0	21.0	38.0
140-144	31.580422529546617	36.0	27.4	38.0	16.8	38.0
145-149	31.468567685740062	35.0	28.0	37.6	17.8	38.0
150-154	30.493722959890146	35.0	25.6	37.0	14.0	38.0
155-159	30.36737203169259	35.0	25.4	37.0	14.2	37.4
160-164	29.61311312461576	34.4	24.2	37.0	10.2	37.4
165-169	28.515284377208456	33.0	22.6	36.6	9.8	37.0
170-174	27.88596011422202	32.2	21.4	36.2	9.4	37.0
175-179	27.0635000890564	29.8	20.2	35.6	9.2	37.0
180-184	26.197116296172872	28.2	20.0	35.0	9.0	37.0
185-189	25.46987537848972	27.4	18.6	35.0	9.0	36.6
190-194	24.437780024935794	25.8	16.0	34.6	8.6	36.0
195-199	23.56544439145748	24.8	15.0	33.6	8.0	36.0
200-204	22.72370738938333	23.4	14.6	32.4	8.0	36.0
205-209	21.758782972415496	22.6	11.0	31.0	8.0	35.2
210-214	20.465671916206542	20.4	9.0	28.6	8.0	35.0
215-219	19.88730515320574	20.0	9.0	27.8	8.0	34.0
220-224	19.209030319396486	19.0	9.0	26.2	8.0	33.8
225-229	18.421744126586496	17.2	9.0	25.6	8.0	32.4
230-234	17.456725482197335	15.8	9.0	24.6	7.6	31.4
235-239	16.424761702298802	14.8	8.2	22.8	7.0	29.4
240-244	15.579966330933598	13.6	8.0	21.4	7.0	28.6
245-249	14.85778036967026	12.8	8.0	20.2	7.0	26.8
250-254	14.141145782461058	12.0	8.0	19.2	7.0	25.6
255-259	13.549723350589208	11.2	8.0	18.0	7.0	24.4
260-264	12.929932144765493	10.2	7.8	16.4	7.0	23.2
265-269	12.566588335334709	9.0	7.2	15.8	7.0	22.4
270-274	12.049567645521037	8.8	7.0	14.4	7.0	21.2
275-279	11.553570587255166	8.0	7.0	13.8	7.0	20.4
280-284	11.423619482093919	8.0	7.0	13.4	7.0	19.8
285-289	11.169650726527893	8.0	7.0	13.0	7.0	19.0
290-294	10.87488781765845	8.0	7.0	13.0	7.0	19.0
295-299	10.588452544427657	8.0	7.0	13.0	7.0	18.0
300-301	10.29862048756945	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.01597018577513154
1101	2	-0.019593157763068803
1101	3	0.04460071438218449
1101	4	0.11542455635983373
1101	5	0.09190626299114513
1101	6	0.07158323767629327
1101	7	0.8983730974445052
1101	8	0.2961254255624297
1101	9	0.29747133744978527
1101	10-14	0.15531739551025936
1101	15-19	0.2607800861768226
1101	20-24	0.17256412260128684
1101	25-29	0.1580461872202008
1101	30-34	0.5164980125599499
1101	35-39	0.2564269819128171
1101	40-44	0.5512324659242935
1101	45-49	0.24421396012876784
1101	50-54	0.14258731430081895
1101	55-59	0.4244505228100621
1101	60-64	0.2815267828134296
1101	65-69	0.3409374275179786
1101	70-74	0.2015434126340807
1101	75-79	0.3560099267340249
1101	80-84	0.7691933105334456
1101	85-89	0.45508188670035565
1101	90-94	0.457127146105087
1101	95-99	1.063260826681116
1101	100-104	0.5646427292428982
1101	105-109	0.598696177116409
1101	110-114	0.5550372285075085
1101	115-119	0.5976667308453258
1101	120-124	1.1100998134631013
1101	125-129	0.7968907884445784
1101	130-134	1.0587616884374
1101	135-139	0.7721334063108074
1101	140-144	1.5391783901943725
1101	145-149	1.6413681137934049
1101	150-154	1.367262102877838
1101	155-159	1.2411740763604016
1101	160-164	1.4446949228425652
1101	165-169	0.8075244260637611
1101	170-174	1.0712402100913074
1101	175-179	1.1266766405872026
1101	180-184	0.812005199834541
1101	185-189	0.7770272726303169
1101	190-194	1.2009709427292456
1101	195-199	1.201432371359875
1101	200-204	1.3124566128832278
1101	205-209	0.9872714327044143
1101	210-214	0.926761559537475
1101	215-219	1.4908155411426094
1101	220-224	1.3347092383170747
1101	225-229	0.43964135611777166
1101	230-234	0.7386154552761326
1101	235-239	0.41354925021476774
1101	240-244	0.5709227361643077
1101	245-249	0.4348641935964537
1101	250-254	0.37262889977115954
1101	255-259	0.18973298155725615
1101	260-264	0.06997607483661206
1101	265-269	0.1318815323396425
1101	270-274	0.1027193372589128
1101	275-279	0.3581794584486371
1101	280-284	0.006766217623388471
1101	285-289	0.035086562275880695
1101	290-294	-0.11124339597798993
1101	295-299	0.013145373050468478
1101	300-301	-0.23112172907347883
1102	1	0.06902261459593717
1102	2	-0.010037990123272778
1102	3	-0.004149059890977469
1102	4	0.01952134802900929
1102	5	0.07903441564268121
1102	6	0.07307002513189786
1102	7	0.9673010454370399
1102	8	0.05468679316611258
1102	9	0.1411768692622246
1102	10-14	0.20561439183843078
1102	15-19	0.1557635328751914
1102	20-24	0.10174344000332525
1102	25-29	0.15789257258105494
1102	30-34	0.416449857500659
1102	35-39	0.17492622949000491
1102	40-44	0.4425029167760428
1102	45-49	0.21286309032799267
1102	50-54	0.1968520467392807
1102	55-59	0.33282300219367755
1102	60-64	0.27192875717086196
1102	65-69	0.27556332290110674
1102	70-74	0.2574740693486959
1102	75-79	0.21354029451828893
1102	80-84	0.8029584342317477
1102	85-89	0.35574088868675346
1102	90-94	0.5283942261026837
1102	95-99	0.9695898461237178
1102	100-104	0.7149262421544762
1102	105-109	0.7726779985815213
1102	110-114	0.7330893563591871
1102	115-119	0.7810614217500387
1102	120-124	0.8823909107965093
1102	125-129	0.8184671443104676
1102	130-134	0.8981741967140593
1102	135-139	1.1252566230687648
1102	140-144	1.774454378877934
1102	145-149	1.8403790088755585
1102	150-154	1.8144189077896584
1102	155-159	1.315837773465077
1102	160-164	1.476668196784658
1102	165-169	1.5437709799673343
1102	170-174	1.7539746951331416
1102	175-179	1.3344719815852137
1102	180-184	1.2889485574460444
1102	185-189	1.209480170962948
1102	190-194	1.4526138729646014
1102	195-199	1.8531179188227043
1102	200-204	1.9566388797826448
1102	205-209	1.2105775183000311
1102	210-214	1.810674158104863
1102	215-219	1.7645532164571236
1102	220-224	1.2776293611124707
1102	225-229	1.0665359567317516
1102	230-234	1.2036536783544456
1102	235-239	0.746355125132002
1102	240-244	0.911888485878384
1102	245-249	0.6374467494262266
1102	250-254	0.9958018366278392
1102	255-259	0.5236302908933173
1102	260-264	0.4985033727358452
1102	265-269	0.39073520115322147
1102	270-274	0.327150565814863
1102	275-279	0.5139487957146347
1102	280-284	0.36825035655073357
1102	285-289	0.20270132180155187
1102	290-294	0.059606661206141
1102	295-299	0.05004803963687543
1102	300-301	-0.06280836002514434
1103	1	-0.0133731100044443
1103	2	-0.04601426569605849
1103	3	-0.01748322701061511
1103	4	0.0791523013834734
1103	5	0.04593229766512508
1103	6	0.12764534730212063
1103	7	0.9220553109755301
1103	8	0.23432284544450965
1103	9	0.19457366656075692
1103	10-14	0.014537989754600744
1103	15-19	0.04375364288689099
1103	20-24	0.06329274323056211
1103	25-29	0.04778852567095271
1103	30-34	0.5144199085379952
1103	35-39	0.1653777728765604
1103	40-44	0.4571055614820594
1103	45-49	0.1371166511567239
1103	50-54	0.12959916416365047
1103	55-59	0.22210997059477933
1103	60-64	0.2182366071085724
1103	65-69	0.3161916450184066
1103	70-74	0.21550126681655257
1103	75-79	0.2509678392902046
1103	80-84	0.7259839240411168
1103	85-89	0.48647970352366343
1103	90-94	0.28315715019124355
1103	95-99	0.8723687596680989
1103	100-104	0.4795233550090572
1103	105-109	0.6292443062390447
1103	110-114	0.6920146378823162
1103	115-119	0.6537307084298192
1103	120-124	0.980385494392408
1103	125-129	0.9941399858060862
1103	130-134	1.1872317135380968
1103	135-139	1.1670976232275052
1103	140-144	1.4373034266779285
1103	145-149	1.492895520249526
1103	150-154	2.0781833567451002
1103	155-159	0.8037831476346966
1103	160-164	1.1299476226265845
1103	165-169	1.9955034115287091
1103	170-174	2.0538457789430957
1103	175-179	1.7623457784075285
1103	180-184	1.3720845880780708
1103	185-189	1.5080750806975658
1103	190-194	1.627129835963732
1103	195-199	1.9682424816861896
1103	200-204	1.9081019342304941
1103	205-209	1.601585133013792
1103	210-214	1.8810070793553528
1103	215-219	1.8418428016644697
1103	220-224	1.6240084035730398
1103	225-229	0.935899601404099
1103	230-234	1.4504863326329662
1103	235-239	1.2273457594669992
1103	240-244	0.5275667149162739
1103	245-249	0.9317556768723882
1103	250-254	0.8886912428918183
1103	255-259	0.5463748588557191
1103	260-264	0.6248349855832114
1103	265-269	0.3996260889913277
1103	270-274	0.4440544607193093
1103	275-279	0.4466011501187097
1103	280-284	0.5337075520463639
1103	285-289	0.08516583377199893
1103	290-294	0.17709260308802932
1103	295-299	0.18008647397861033
1103	300-301	0.08088749397245998
2116	1	0.14461305324152818
2116	2	0.09734859682574637
2116	3	0.1655661718182273
2116	4	0.18507045894691032
2116	5	0.14996089516342437
2116	6	0.2760058420988969
2116	7	1.1243595116662135
2116	8	0.41239078182778144
2116	9	0.29551151666643705
2116	10-14	0.2194700187217009
2116	15-19	0.2942935996903415
2116	20-24	0.2890850071221678
2116	25-29	0.2731414321977894
2116	30-34	0.5256496240645063
2116	35-39	0.10806797049497874
2116	40-44	0.5629809844963489
2116	45-49	0.24044809800995637
2116	50-54	0.3288223193593609
2116	55-59	0.4814841981966822
2116	60-64	0.5496903902711594
2116	65-69	0.64559416982177
2116	70-74	0.6595645139492916
2116	75-79	0.5114219804990228
2116	80-84	0.9647880496575922
2116	85-89	0.645396093955739
2116	90-94	0.7559911437926132
2116	95-99	1.3384901950869548
2116	100-104	0.8322305309483511
2116	105-109	0.7902209639353046
2116	110-114	1.0085247267008839
2116	115-119	0.9394340382596909
2116	120-124	1.1539986427736935
2116	125-129	1.1604496842387704
2116	130-134	1.3826466429105935
2116	135-139	1.6443993406943918
2116	140-144	2.050662708737512
2116	145-149	2.1713614654338045
2116	150-154	2.3006640395738955
2116	155-159	2.295248942670561
2116	160-164	2.051154815890694
2116	165-169	1.9045141644652652
2116	170-174	1.841216218184961
2116	175-179	2.469453342422735
2116	180-184	2.1056256652197085
2116	185-189	1.6808804306011176
2116	190-194	1.7248547409659842
2116	195-199	1.0532629827199003
2116	200-204	1.4594894210925062
2116	205-209	1.2344851919181714
2116	210-214	2.280038044578667
2116	215-219	2.2374598539045714
2116	220-224	1.553436800103455
2116	225-229	1.1610866516807157
2116	230-234	1.2031334492058932
2116	235-239	0.9225927536112088
2116	240-244	0.8087016059431722
2116	245-249	1.174345621548472
2116	250-254	0.824112640196077
2116	255-259	1.4938011995078249
2116	260-264	0.7754236828724554
2116	265-269	0.7206671950076569
2116	270-274	0.5103805599789588
2116	275-279	0.2548477631169419
2116	280-284	0.3634258505183148
2116	285-289	0.27843849174545454
2116	290-294	0.3785869263229191
2116	295-299	0.2816743512265045
2116	300-301	-0.17849562703561261
1117	1	0.0948926261308003
1117	2	-0.08597341424104599
1117	3	0.10644536033106533
1117	4	0.1038636795220853
1117	5	0.074846075768086
1117	6	0.06978730493573693
1117	7	0.7727282343403559
1117	8	0.32100789761102533
1117	9	0.4051075730339022
1117	10-14	0.11801763320723069
1117	15-19	0.1733381165198722
1117	20-24	0.2550192683007353
1117	25-29	0.1287241224844493
1117	30-34	0.4293072387242276
1117	35-39	0.1963252123908461
1117	40-44	0.5315213411187116
1117	45-49	0.22628609089144192
1117	50-54	0.21371446751267342
1117	55-59	0.403461820752959
1117	60-64	0.24898260345491252
1117	65-69	0.37313979274964737
1117	70-74	0.2874284377243157
1117	75-79	0.5589153468120855
1117	80-84	1.0024697139727081
1117	85-89	0.6404266060124968
1117	90-94	0.501193231734689
1117	95-99	1.194926642333904
1117	100-104	0.5844930681811888
1117	105-109	0.7127566871378264
1117	110-114	0.783088993627679
1117	115-119	0.8623127971070232
1117	120-124	1.1575068035150409
1117	125-129	1.201656231411981
1117	130-134	1.151646625491722
1117	135-139	1.4028093460942372
1117	140-144	1.6802694486795673
1117	145-149	2.0801764595984835
1117	150-154	2.26231929687966
1117	155-159	1.9789547495417281
1117	160-164	2.340269298521168
1117	165-169	2.147074738591268
1117	170-174	1.769643003764454
1117	175-179	2.086157111285914
1117	180-184	2.0335751737172103
1117	185-189	1.4940727872002633
1117	190-194	-0.557435724524332
1117	195-199	1.9527193977999957
1117	200-204	1.9473423129155023
1117	205-209	2.0200423925893816
1117	210-214	2.290794778424111
1117	215-219	1.9889643218451383
1117	220-224	1.7236732903169525
1117	225-229	1.8979035270835283
1117	230-234	1.858952339391518
1117	235-239	1.2134382075760364
1117	240-244	1.106200372106434
1117	245-249	1.204803331431357
1117	250-254	0.7134596066995869
1117	255-259	1.0131841069497476
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2108	255-259	-0.7833678289651118
2108	260-264	-0.7229884756263374
2108	265-269	-0.44722464320623345
2108	270-274	-0.43931279519370925
2108	275-279	-0.3731526249688262
2108	280-284	-0.786433240105417
2108	285-289	-1.2220309778896858
2108	290-294	-0.7644028179399758
2108	295-299	-0.5620419124207015
2108	300-301	2.526665342950519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	56.0
11	348.0
12	640.0
13	616.0
14	693.0
15	1248.0
16	1738.0
17	2611.0
18	3348.0
19	3604.0
20	4697.0
21	6337.0
22	8604.0
23	12393.0
24	17620.0
25	25326.0
26	36451.0
27	49369.0
28	58651.0
29	55935.0
30	37676.0
31	16086.0
32	3688.0
33	345.0
34	7.0
35	6.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.345791653978523	22.047492918579465	13.644015696909456	35.962699730532556
2	46.53944049595799	23.58759415560165	16.33294454946078	13.540020798979585
3	45.51845191241446	22.56459462099318	18.235304256896125	13.68164920969623
4	45.99619642970003	21.400828511838757	18.186179595166823	14.416795463294395
5	27.434256264112566	21.51229265658127	37.248559297201325	13.804891782104834
6	26.543404942343162	20.786052043413562	37.61685062080932	15.053692393433957
7	16.32518802392457	49.62423942957936	19.156894402086795	14.89367814440927
8	25.63330594609502	19.90037173866829	38.78607502571145	15.680247289525242
9	15.223186840336231	18.349066631427142	39.94984113486587	26.477905393370754
10-14	26.917269473188277	34.22776606318983	23.378627612081793	15.4763368515401
15-19	20.66872741271036	36.63843674409786	21.096140697627654	21.59669514556413
20-24	26.016420851838873	30.75019965871288	26.12104776296058	17.112331726487675
25-29	19.09754261779864	25.26696811780726	38.90655972237384	16.72892954202026
30-34	19.07547961182899	28.014559285710185	31.64972679793389	21.260234304526936
35-39	20.51871046326567	30.075209569828836	28.10803978235764	21.29804018454785
40-44	20.469298522812803	28.8054376116796	29.007107275620953	21.71815658988664
45-49	20.7448562744546	30.684470286761623	26.512895941900748	22.057777496883027
50-54	22.079840502852676	29.64480858618649	27.66453888892081	20.610812022040022
55-59	21.37991462076336	26.903020448499543	30.24470401673111	21.472360914005986
60-64	22.484386401374344	25.34200532040196	28.31775325055876	23.85585502766494
65-69	20.017581457882066	27.6467850638046	28.601642085183887	23.733991393129443
70-74	20.848908628129184	26.551391290858216	29.424293437979397	23.175406643033202
75-79	20.150246772423543	26.320246829879284	30.35582342700535	23.173682970691825
80-84	23.00551000591794	25.614632829063417	27.932167747792263	23.447689417226382
85-89	21.05264669888019	27.819726855389636	26.377185472889508	24.75044097284067
90-94	20.00258550851207	30.168000597539745	29.10392020546174	20.725493688486445
95-99	21.97653507385936	30.688319821657366	26.61993599430039	20.71520911018288
100-104	22.951903796101053	27.98531431165145	26.573109562359594	22.489672329887902
105-109	23.534332680253033	27.349164306193153	23.674122507138875	25.442380506414935
110-114	21.510454072750466	27.545433130131514	29.993967146805172	20.950145650312844
115-119	23.776968290174494	26.662625612621877	27.617367722511737	21.94303837469189
120-124	17.815647497515037	29.012565571368654	28.0596620453096	25.112124885806708
125-129	20.482226065373148	24.51188472079381	30.360017696369372	24.645871517463675
130-134	25.161651737748997	28.87926824363534	25.66881359632743	20.290266422288234
135-139	24.470803863324274	26.738582107131982	24.814906318408244	23.975707711135495
140-144	20.899412227731588	29.094210184605306	26.701063505834632	23.30531408182847
145-149	21.034088493338007	26.230730779617	29.258763437462292	23.4764172895827
150-154	23.072101214039886	25.059495423649935	25.259096680781628	26.609306681528555
155-159	21.386234752681744	26.755818830545774	30.315719317195928	21.542227099576554
160-164	25.516958063051938	28.508161588536428	25.66846886185915	20.306411486552484
165-169	20.285727418455934	25.066734847483723	28.467138186811606	26.180399547248733
170-174	22.8766367705275	28.379403264635418	26.383965250765595	22.359994714071487
175-179	20.715898579119433	28.33464523950427	29.09949611311887	21.849960068257424
180-184	20.163576505196872	29.287548765563326	26.392755979706635	24.156118749533174
185-189	22.37073893833275	27.36559664918097	27.56732376886703	22.696340643619255
190-194	20.98473400862985	27.34583187299982	26.195108217895168	25.474325900475158
195-199	21.758490522674908	29.308003010681023	27.507167604152897	21.426338862491164
200-204	22.52678874097227	27.232299321447655	27.43477336581498	22.806138571765096
205-209	22.24651961826403	26.721460295207617	27.558992685883698	23.473027400644654
210-214	24.198463633386382	26.527374789568338	27.226496291231676	22.047665285813604
215-219	24.333714456439928	26.68796359604015	27.00339563451252	21.974926313007405
220-224	20.981056840968243	26.516688021051788	27.92033186438146	24.58192327359851
225-229	23.129786781731372	27.72291392554885	25.98229213947957	23.165007153240218
230-234	22.598493510373636	26.308353490723768	27.841042936678022	23.25211006222457
235-239	23.530425689612574	26.413440047803178	25.389291398300458	24.666842864283787
240-244	24.38720575476739	30.380414485742357	22.563215683120077	22.669164076370176
245-249	22.895597166282673	28.71603647290674	23.48038173596787	24.907984624842715
250-254	23.34495854568019	26.454118715059728	24.745787057518946	25.455135681741137
255-259	23.610461541997278	27.35651864151637	25.919320643274517	23.11369917321183
260-264	23.566392985802686	28.05834056318121	25.07747907174499	23.297787379271117
265-269	22.54322108396008	27.71734071831172	25.71156067039363	24.02787752733457
270-274	22.11879549776784	29.59562646871247	23.379604359741908	24.905973673777773
275-279	23.50393859130005	28.484259998735972	22.959315587168984	25.05248582279499
280-284	21.105735806994662	29.326791039202053	25.407275046395515	24.16019810740777
285-289	22.425436807299178	28.21462018879958	26.10180008848185	23.2581429154194
290-294	21.98722184237591	30.279579653771684	23.548466793452345	24.184731710400065
295-299	21.170718254264653	31.394795658643933	21.846282900595813	25.5882031864956
300-301	17.229828724425012	32.50084747223451	29.57735554189386	20.69196826144662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.0
25	0.25
26	0.25
27	0.0
28	1.0
29	0.75
30	1.5
31	1.0
32	1.25
33	7.25
34	21.25
35	75.5
36	274.25
37	879.25
38	1988.5
39	3217.75
40	4328.25
41	5653.0
42	8269.25
43	11907.75
44	14263.5
45	14084.5
46	12252.5
47	10014.75
48	7894.75
49	6326.5
50	4899.75
51	3886.5
52	2763.25
53	1556.75
54	674.0
55	264.25
56	126.0
57	90.75
58	62.0
59	50.75
60	44.0
61	36.5
62	31.25
63	22.25
64	23.75
65	13.5
66	11.25
67	8.25
68	5.0
69	4.5
70	4.5
71	3.75
72	3.75
73	2.75
74	2.75
75	2.25
76	2.5
77	1.25
78	2.25
79	1.75
80	1.5
81	1.25
82	1.5
83	1.0
84	1.25
85	0.25
86	0.5
87	0.75
88	0.5
89	0.25
90	0.25
91	0.75
92	0.75
93	0.0
94	0.25
95	0.75
96	0.5
97	1.25
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	348094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.852588289123952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.36408740268059	6.976729673467882
2	5.14319761790673	1.4249319818092996
3	4.8516063342385625	2.016219152673381
4	4.667243276383171	2.5861359821167205
5	4.266823568572066	2.9553275098879737
6	3.9880158938143175	3.3146605360495442
7	3.967584772326242	3.8472922847263114
8	3.136205383452005	3.4755649573675793
9	2.6230521834078995	3.2702455761873557
>10	16.498509600917895	40.09657331183253
>50	0.38501767892864863	3.4002369340360423
>100	0.06406442251132974	1.4563457222596978
>500	0.010617110681837464	0.9895169876848671
>1k	0.019110799225566563	6.830726363387695
>5k	0.010617110680870312	9.292164577530675
>10k+	0.0042468442723481254	8.067328448982472
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14906	4.2821766534326935	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	12520	3.596729619010957	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7683	2.2071624331358772	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	7335	2.107189437335892	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	5902	1.6955190264698616	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	5436	1.561647141289422	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5234	1.5036168391296603	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4553	1.307980028383138	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4391	1.2614408751659034	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	3208	0.9215901451906668	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2502	0.718771366355065	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	2423	0.6960763471935741	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1782	0.5119306853895788	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1771	0.5087706194303838	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1523	0.43752549598671625	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT	1069	0.3071009554890346	Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	929	0.26688193419019	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	657	0.18874212138100627	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	647	0.18586933414537454	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	624	0.1792619235034215	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	507	0.14565031284652996	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	441	0.12668991709136038	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT	439	0.12611535964423404	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	353	0.10140938941780094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.872787235631755E-4	0.0	0.0
2	2.872787235631755E-4	0.0	0.0
3	2.872787235631755E-4	0.0	0.0
4	2.872787235631755E-4	0.0	0.0
5	2.872787235631755E-4	0.0	0.0
6	2.872787235631755E-4	0.0	0.0
7	2.872787235631755E-4	0.0	0.0
8	5.74557447126351E-4	0.0	0.0
9	5.74557447126351E-4	0.0	0.0
10-14	0.002068406809654863	0.0	0.0
15-19	0.002872787235631755	0.0	0.0
20-24	0.003332433193332835	0.0	0.0
25-29	0.003792079151033916	0.0	0.0
30-34	0.007067056599654116	0.0	0.0
35-39	0.00787143702563101	0.0	0.0
40-44	0.009422742132872156	0.0	0.0
45-49	0.011203870218963843	0.0	0.0
50-54	0.015857785540687286	0.0	0.0
55-59	0.020166966394134917	0.0	0.0
60-64	0.02688928852551322	0.0	0.0
65-69	0.036082207679534836	0.0	0.0
70-74	0.05079087832596942	0.0	0.0
75-79	0.07078547748596643	0.0	0.0
80-84	0.1003751860129735	0.0	0.0
85-89	0.13858325624687584	0.0	0.0
90-94	0.18391583882514492	0.0	0.0
95-99	0.2411417605589295	0.0	0.0
100-104	0.31905175038926264	0.0	0.0
105-109	0.4170137951243055	0.0	0.0
110-114	0.5404287347670458	0.0	0.0
115-119	0.6899285825093223	0.0	0.0
120-124	0.8625830953707906	0.0	0.0
125-129	1.0581624503726004	0.0	0.0
130-134	1.2954546760357837	0.0	0.0
135-139	1.5496388906444811	0.0	0.0
140-144	1.8373198044206451	0.0	0.0
145-149	2.192568673978868	0.0	0.0
150-154	2.5953334444144396	0.0	0.0
155-159	3.042166771044603	0.0	0.0
160-164	3.5593833849477443	0.0	0.0
165-169	4.132504438456278	0.0	0.0
170-174	4.762047033272622	0.0	0.0
175-179	5.441518670244244	0.0	0.0
180-184	6.166495257028274	0.0	0.0
185-189	6.962774423000684	0.0	0.0
190-194	7.834722804759633	0.0	0.0
195-199	8.708222491625827	0.0	0.0
200-204	9.552362292943858	0.0	0.0
205-209	10.390124506598791	0.0	0.0
210-214	11.226392870891196	0.0	0.0
215-219	12.079553224129114	0.0	0.0
220-224	12.905594465862668	0.0	0.0
225-229	13.696013145874392	0.0	0.0
230-234	14.430527386280719	0.0	0.0
235-239	15.080064580257055	0.0	0.0
240-244	15.646348400144788	0.0	0.0
245-249	16.08352916166323	0.0	0.0
250-254	16.471355438473516	0.0	0.0
255-259	16.79069446758634	0.0	0.0
260-264	17.06452854688676	0.0	0.0
265-269	17.277229713812936	0.0	0.0
270-274	17.421558544531074	0.0	0.0
275-279	17.515843421604508	0.0	0.0
280-284	17.578125448873003	0.0	0.0
285-289	17.630467632306214	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6675	0.0	291.46442	1
GGGTTAT	6675	0.0	291.46442	2
GTTATTA	6740	0.0	287.55933	4
GGTTATT	6785	0.0	286.08698	3
TTAGTAG	6885	0.0	284.50253	8
TATTAGT	6815	0.0	284.17828	6
TAGTAGA	6945	0.0	281.83224	9
TTATTAG	6920	0.0	279.01373	5
GGGGAGC	3610	0.0	278.65652	3
GGGGGGA	3605	0.0	277.40637	1
ATTAGTA	7065	0.0	273.91364	7
GGAGCGA	2955	0.0	271.04062	5
GGGAGCG	2985	0.0	269.79898	4
GCGAAAG	3060	0.0	262.70422	8
CGAAAGC	3085	0.0	260.57538	9
GAGCGAA	3120	0.0	257.17947	6
AGCGAAA	3170	0.0	252.65773	7
GGGGGAG	4755	0.0	213.10725	2
GGGAGCA	1035	0.0	192.39131	4
CAAAAGC	1410	0.0	112.97872	9
>>END_MODULE