##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6628_flu.3520000003bad3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439231
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16855595347323	34.0	34.0	34.0	34.0	34.0
2	33.369038159874876	34.0	34.0	34.0	34.0	34.0
3	33.39950049063022	34.0	34.0	34.0	34.0	34.0
4	33.395195238951715	34.0	34.0	34.0	34.0	34.0
5	33.342787735838314	34.0	34.0	34.0	34.0	34.0
6	36.84530008127842	38.0	38.0	38.0	36.0	38.0
7	36.11043164075395	38.0	38.0	38.0	34.0	38.0
8	36.76997069878948	38.0	38.0	38.0	35.0	38.0
9	36.82920832090631	38.0	38.0	38.0	36.0	38.0
10-14	36.82291550459781	38.0	38.0	38.0	36.0	38.0
15-19	36.719313072164766	38.0	38.0	38.0	35.6	38.0
20-24	36.810574845582394	38.0	38.0	38.0	35.8	38.0
25-29	36.71925251177627	38.0	38.0	38.0	35.6	38.0
30-34	36.38309226807762	38.0	37.6	38.0	33.2	38.0
35-39	36.65590042597175	38.0	38.0	38.0	34.8	38.0
40-44	36.1848517067329	38.0	37.2	38.0	33.2	38.0
45-49	36.48831571542081	38.0	37.8	38.0	34.0	38.0
50-54	36.44136820943877	38.0	37.6	38.0	34.0	38.0
55-59	36.089450881199184	38.0	37.0	38.0	33.0	38.0
60-64	36.27705011713654	38.0	37.0	38.0	33.8	38.0
65-69	36.11958445555983	38.0	37.0	38.0	33.0	38.0
70-74	35.98866928791456	38.0	37.0	38.0	32.0	38.0
75-79	36.02210636316654	38.0	37.0	38.0	32.0	38.0
80-84	35.312215667837656	38.0	36.6	38.0	28.8	38.0
85-89	35.51873706546214	38.0	37.0	38.0	30.0	38.0
90-94	35.49654783018502	38.0	37.0	38.0	29.6	38.0
95-99	34.579100746532006	38.0	35.4	38.0	24.4	38.0
100-104	34.91025178095352	38.0	36.0	38.0	25.6	38.0
105-109	34.67754416241112	38.0	35.4	38.0	24.4	38.0
110-114	34.19573709505932	38.0	34.4	38.0	24.0	38.0
115-119	34.1877554179919	37.8	34.6	38.0	23.4	38.0
120-124	33.71150032670736	37.0	33.2	38.0	22.2	38.0
125-129	33.30750971584429	37.0	32.0	38.0	22.4	38.0
130-134	33.06612830150877	37.0	31.6	38.0	21.6	38.0
135-139	32.32528851561024	36.8	29.8	38.0	18.8	38.0
140-144	31.171866739824832	35.8	26.6	38.0	15.4	38.0
145-149	30.93295008776703	35.0	26.8	37.6	15.2	38.0
150-154	29.8602972012449	35.0	23.8	37.0	10.2	38.0
155-159	29.873132816217435	35.0	24.2	37.0	10.0	37.4
160-164	29.08539287982861	33.6	23.2	37.0	10.2	37.4
165-169	27.921534682205948	32.0	21.2	36.6	9.6	37.0
170-174	27.3228269407214	30.8	20.4	36.2	9.2	37.0
175-179	26.570334061120455	29.0	20.0	35.4	9.0	37.0
180-184	25.710967122083822	27.6	18.8	35.0	9.0	36.8
185-189	24.934351628186537	26.0	17.0	35.0	8.4	36.4
190-194	23.98939874462413	25.2	15.6	34.2	8.0	36.0
195-199	23.23205602518948	24.4	14.8	33.4	8.0	36.0
200-204	22.475021116451252	23.2	13.4	31.8	8.0	36.0
205-209	21.495255116328313	22.2	10.0	30.6	8.0	35.2
210-214	20.38452203965567	20.4	9.0	28.2	8.0	35.0
215-219	19.878353303842395	20.0	9.0	27.8	8.0	34.2
220-224	19.27005607527702	19.0	9.0	26.4	8.0	33.8
225-229	18.48516384317136	17.8	9.0	25.8	8.0	32.8
230-234	17.590410968260436	16.0	9.0	24.8	8.0	31.4
235-239	16.572715495946323	14.8	8.8	23.0	7.4	29.4
240-244	15.854035348142549	14.0	8.0	21.8	7.0	28.8
245-249	15.190107255635416	13.2	8.0	20.8	7.0	27.4
250-254	14.461688268815271	12.2	8.0	19.6	7.0	26.2
255-259	13.966759176834056	11.8	8.0	18.8	7.0	25.0
260-264	13.355130216218802	10.6	8.0	17.6	7.0	23.8
265-269	12.883606576038577	9.8	8.0	16.4	7.0	23.2
270-274	12.384007048682813	9.0	8.0	15.6	7.0	21.8
275-279	11.91372557947868	9.0	7.0	14.2	7.0	21.0
280-284	11.777249784282075	8.4	7.0	14.0	7.0	20.8
285-289	11.477341535547355	8.0	7.0	13.0	7.0	19.8
290-294	11.163204782904668	8.0	7.0	13.0	7.0	19.0
295-299	10.839595110545478	8.0	7.0	13.0	7.0	19.0
300-301	10.513601043642183	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.009122339820685
1101	2	-0.03298911278891836
1101	3	0.029946892273699177
1101	4	-0.00484090034413498
1101	5	0.09903216260295267
1101	6	0.07947639511379379
1101	7	0.8942625885234676
1101	8	0.21768702520643757
1101	9	0.07710066331680565
1101	10-14	-0.01676539443416658
1101	15-19	0.18225920844339072
1101	20-24	0.12043508050198426
1101	25-29	0.05456943821570803
1101	30-34	0.4157156440901062
1101	35-39	0.1973277367591848
1101	40-44	0.527405114141871
1101	45-49	0.15930077320292924
1101	50-54	0.13603757993935517
1101	55-59	0.26263693143503275
1101	60-64	0.2105178252774138
1101	65-69	0.23971907552525806
1101	70-74	0.1638945496610873
1101	75-79	0.2896043875888452
1101	80-84	0.6412391766915917
1101	85-89	0.3317692338046285
1101	90-94	0.30546059514934853
1101	95-99	0.8654379607443659
1101	100-104	0.4007755085173912
1101	105-109	0.5742104804961272
1101	110-114	0.4633575135349446
1101	115-119	0.3762011550274664
1101	120-124	0.6933593141224819
1101	125-129	0.7228147852950428
1101	130-134	0.6382802017508382
1101	135-139	0.6283327052184688
1101	140-144	1.0868584715822074
1101	145-149	1.4476595518715776
1101	150-154	1.01854942910904
1101	155-159	0.760394542017842
1101	160-164	1.1199177818805843
1101	165-169	1.0593542716500757
1101	170-174	0.6604317609219414
1101	175-179	0.7979685216707857
1101	180-184	0.6199353085170962
1101	185-189	0.7079689724748732
1101	190-194	0.8717896581072253
1101	195-199	0.8504858061172378
1101	200-204	0.9488416125371479
1101	205-209	0.4455742251356831
1101	210-214	0.8104926195536706
1101	215-219	0.7132788542251589
1101	220-224	0.5494697926141789
1101	225-229	0.4091589135603009
1101	230-234	0.3928618034815017
1101	235-239	0.6290119833142072
1101	240-244	0.18524903771933765
1101	245-249	0.21584143232897546
1101	250-254	0.46106928213808906
1101	255-259	0.14854577733389185
1101	260-264	0.20122211607173135
1101	265-269	0.07495520606584982
1101	270-274	0.09988062374982576
1101	275-279	0.27022933533706706
1101	280-284	-0.06542715416336442
1101	285-289	0.27831329390542514
1101	290-294	-0.13716970799810646
1101	295-299	-0.10354036600293348
1101	300-301	-0.28400771667360836
1102	1	0.00564248634083242
1102	2	0.06168910871856781
1102	3	0.1507024240048409
1102	4	0.22638099429239134
1102	5	0.15881208457799545
1102	6	-0.07492814343708432
1102	7	1.0074866273572667
1102	8	0.23285870989031565
1102	9	0.249007212052625
1102	10-14	0.23016870128041234
1102	15-19	0.23215765687842804
1102	20-24	0.14675361291808287
1102	25-29	0.07542205443417771
1102	30-34	0.42538388912421254
1102	35-39	0.21107799733871246
1102	40-44	0.5867008049009783
1102	45-49	0.1375606975604171
1102	50-54	0.17158003206716899
1102	55-59	0.1564321605961112
1102	60-64	0.2721423525116933
1102	65-69	0.21681190139856454
1102	70-74	0.10996501036569128
1102	75-79	0.37603787280281864
1102	80-84	0.867933837044788
1102	85-89	0.3957315316693695
1102	90-94	0.4734530904589107
1102	95-99	1.0813970423132062
1102	100-104	0.7418053188154943
1102	105-109	0.9034114692093098
1102	110-114	0.8775791997078457
1102	115-119	0.693088495329441
1102	120-124	1.0069931328782502
1102	125-129	0.7549798289235028
1102	130-134	0.9424265432142533
1102	135-139	1.0487448675818527
1102	140-144	1.7419565983876488
1102	145-149	1.7654164832870443
1102	150-154	1.7630081682019316
1102	155-159	1.0603382568396036
1102	160-164	1.3877786473024294
1102	165-169	1.0486112477363179
1102	170-174	1.3559506864408633
1102	175-179	1.3728775421895634
1102	180-184	1.0903167969473593
1102	185-189	1.2932255316338725
1102	190-194	1.9149169102588637
1102	195-199	1.3082016404184351
1102	200-204	1.5221898361292787
1102	205-209	1.245855353221689
1102	210-214	1.269590865481188
1102	215-219	1.422671033861242
1102	220-224	1.1930288622708112
1102	225-229	1.0013209791369952
1102	230-234	0.747777077908971
1102	235-239	0.7249701714675147
1102	240-244	0.5554509496285647
1102	245-249	0.391947629654684
1102	250-254	0.633355830278294
1102	255-259	0.406257740167808
1102	260-264	0.39892365572448796
1102	265-269	0.24001997869160263
1102	270-274	0.3592632736202823
1102	275-279	0.3596557625683712
1102	280-284	0.14685522470437995
1102	285-289	0.19723762161024005
1102	290-294	0.11252980657945777
1102	295-299	0.15863212275492167
1102	300-301	-0.17656705435489783
1103	1	0.019934269324203058
1103	2	0.08472795726459736
1103	3	0.04518627386822516
1103	4	0.03195916355873152
1103	5	0.08878301403604638
1103	6	0.11407108391409082
1103	7	0.954590526421498
1103	8	0.29091172366477736
1103	9	0.2587859562170536
1103	10-14	0.11093359401247938
1103	15-19	0.19681918636786122
1103	20-24	0.15660192508004656
1103	25-29	0.07145475435336124
1103	30-34	0.5355930667712698
1103	35-39	0.16222705343420074
1103	40-44	0.5351173188634277
1103	45-49	0.19356752410519107
1103	50-54	0.1444094365355113
1103	55-59	0.25727126906002695
1103	60-64	0.19084474653891448
1103	65-69	0.3347172237944349
1103	70-74	0.22145142684413344
1103	75-79	0.3645646298388101
1103	80-84	0.9142785568337786
1103	85-89	0.4453321817601008
1103	90-94	0.4742260353166614
1103	95-99	1.1563684010206003
1103	100-104	0.6007169792999818
1103	105-109	0.740594231739685
1103	110-114	0.757182898949111
1103	115-119	0.8230264169929171
1103	120-124	1.0327289994734699
1103	125-129	0.9390317355306834
1103	130-134	0.9917551380295109
1103	135-139	1.1994676346711586
1103	140-144	1.6909224034966037
1103	145-149	1.7058624289529583
1103	150-154	2.161398335135516
1103	155-159	0.8640112990925424
1103	160-164	1.2414312632071471
1103	165-169	2.050191181365488
1103	170-174	2.2052224427219684
1103	175-179	1.936148451813331
1103	180-184	1.6013369598252893
1103	185-189	1.75819212419335
1103	190-194	1.6586294434516802
1103	195-199	1.6148094160483275
1103	200-204	1.8081439094095408
1103	205-209	1.4196894971948808
1103	210-214	1.9973268860141076
1103	215-219	1.9258966206747061
1103	220-224	1.898483437328757
1103	225-229	1.1993800320057169
1103	230-234	1.6843772454642298
1103	235-239	0.91528216173392
1103	240-244	0.6514673676759841
1103	245-249	1.0308028364492579
1103	250-254	0.8564004514879482
1103	255-259	0.8791716623523023
1103	260-264	0.7881947903341224
1103	265-269	0.4665879969509792
1103	270-274	0.413250555998264
1103	275-279	0.3002273746808992
1103	280-284	0.3299346595162067
1103	285-289	0.3197794290164211
1103	290-294	0.17740566470810926
1103	295-299	0.266204821312348
1103	300-301	-0.013863789987624386
2116	1	0.030995972445168718
2116	2	0.010782947536043253
2116	3	0.03261309220952313
2116	4	-0.005495782057039378
2116	5	-0.01775556237589626
2116	6	0.09842275023762426
2116	7	0.8353903269208303
2116	8	0.2518619325895344
2116	9	0.2317431766623912
2116	10-14	0.1342832914267973
2116	15-19	0.20257541858895678
2116	20-24	0.18849172995795271
2116	25-29	0.057025317565788214
2116	30-34	0.38707003865269485
2116	35-39	-0.024079748436697912
2116	40-44	0.39361117580868665
2116	45-49	0.15172689846181697
2116	50-54	0.1676693876462494
2116	55-59	0.44330114776478524
2116	60-64	0.3436589238451333
2116	65-69	0.47138968023340055
2116	70-74	0.4287030441624182
2116	75-79	0.4854913220423356
2116	80-84	0.8334789013203974
2116	85-89	0.5807120884471431
2116	90-94	0.47684941111153734
2116	95-99	1.2388229261771144
2116	100-104	0.5599981099584141
2116	105-109	0.6519856329129183
2116	110-114	0.7124634322654373
2116	115-119	0.7990225429683093
2116	120-124	0.8801067835661982
2116	125-129	0.9119145781832358
2116	130-134	1.002478153969271
2116	135-139	1.4020347559171071
2116	140-144	1.9914618962197572
2116	145-149	2.1049986026577265
2116	150-154	2.284866631057646
2116	155-159	2.1260836992769647
2116	160-164	1.8559785735727772
2116	165-169	1.8306646184894326
2116	170-174	1.6827668736666084
2116	175-179	2.126451646433768
2116	180-184	2.1639162308939213
2116	185-189	1.7381524560371986
2116	190-194	1.71318664185916
2116	195-199	0.8518775391471962
2116	200-204	0.9942338122979528
2116	205-209	0.9185712205558865
2116	210-214	2.0160274639281113
2116	215-219	1.954685756007489
2116	220-224	1.42835547272853
2116	225-229	0.9235397659274938
2116	230-234	1.6362342255228626
2116	235-239	0.746622904815915
2116	240-244	0.9268723859297161
2116	245-249	1.1110656089865998
2116	250-254	0.9506608926512001
2116	255-259	1.4939496453657473
2116	260-264	0.5927547434746661
2116	265-269	0.819580217004857
2116	270-274	0.6355794073325711
2116	275-279	0.580835793725095
2116	280-284	0.04374906590022931
2116	285-289	0.21356576612035383
2116	290-294	0.4174025040143796
2116	295-299	0.057134935184311786
2116	300-301	-0.036943016110614124
1117	1	0.03593721292711649
1117	2	-0.02109543670086822
1117	3	0.10725906574955957
1117	4	0.03339330080566327
1117	5	0.07745260547352473
1117	6	0.17351534848778272
1117	7	1.0408973405290638
1117	8	0.4590165112929583
1117	9	0.42608748212460057
1117	10-14	0.13229137431569882
1117	15-19	0.1351286862233536
1117	20-24	0.28364359690743157
1117	25-29	0.1525382667727726
1117	30-34	0.42393476228365756
1117	35-39	0.25143314257814353
1117	40-44	0.4858793310304108
1117	45-49	0.1348209166003187
1117	50-54	0.2542888313875338
1117	55-59	0.4022854329761145
1117	60-64	0.44288104035162235
1117	65-69	0.46783239443141156
1117	70-74	0.39563652181227127
1117	75-79	0.558717116292371
1117	80-84	0.876156001838595
1117	85-89	0.5216597184265765
1117	90-94	0.515606834246995
1117	95-99	1.1194965875395653
1117	100-104	0.6649812487845068
1117	105-109	0.6304156961386767
1117	110-114	0.7054105443347822
1117	115-119	0.8150928856327582
1117	120-124	0.9833013868267031
1117	125-129	0.8329916274923477
1117	130-134	1.0130116595513456
1117	135-139	1.1034121639807992
1117	140-144	1.3033911403910956
1117	145-149	1.9500483423933552
1117	150-154	2.2392533004011526
1117	155-159	1.7370198944999657
1117	160-164	1.8468528814499585
1117	165-169	1.561028261201301
1117	170-174	1.1501718531684268
1117	175-179	1.3467096413070436
1117	180-184	1.2826961452932792
1117	185-189	1.3011169384617105
1117	190-194	-0.9440024156899653
1117	195-199	1.3143789575807254
1117	200-204	1.6288070747532508
1117	205-209	1.519767505569373
1117	210-214	1.8772703317533797
1117	215-219	1.5460097436823936
1117	220-224	1.4448500361274164
1117	225-229	1.5310528082419168
1117	230-234	1.439280147853804
1117	235-239	1.1335814632801053
1117	240-244	0.7402139534515157
1117	245-249	1.0586411787604355
1117	250-254	0.7779508262830603
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2108	230-234	-0.9050155328573766
2108	235-239	-1.0126955399317783
2108	240-244	-1.0150158256224504
2108	245-249	-0.9844816633787357
2108	250-254	-0.9391767965695301
2108	255-259	-0.545632864537053
2108	260-264	-0.4284519705254315
2108	265-269	-0.4516269297470519
2108	270-274	-0.48781731933273065
2108	275-279	-0.4721206223975063
2108	280-284	-0.7932340481241056
2108	285-289	-1.2595114454577772
2108	290-294	-0.7215052015643675
2108	295-299	-0.6648891027362858
2108	300-301	2.4299727473068558
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	121.0
11	676.0
12	841.0
13	905.0
14	1154.0
15	1879.0
16	2702.0
17	3712.0
18	5030.0
19	5906.0
20	7443.0
21	9781.0
22	13013.0
23	17173.0
24	23027.0
25	32206.0
26	44078.0
27	57410.0
28	68005.0
29	66391.0
30	48341.0
31	22867.0
32	5879.0
33	657.0
34	28.0
35	0.0
36	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.57047430623066	19.219044193146658	14.24466852294123	35.96581297768145
2	49.289553788325506	22.53620532248407	14.467102731819931	13.707138157370496
3	48.979694056202774	21.78694126780669	16.62678636070769	12.606578315282846
4	49.304124708866176	20.335085638308772	17.128800107460542	13.231989545364511
5	29.689616625420335	20.530427041807158	36.876267840840015	12.903688491932492
6	28.587235418265106	20.11720484209903	37.02949017715052	14.266069562485345
7	15.773476826544574	51.62636517003582	18.607520871705322	13.992637131714291
8	27.927218251899344	18.72090084716243	38.51640708419943	14.835473816738801
9	14.272444340221888	17.5094654065856	39.57097745833058	28.64711279486193
10-14	27.269113518854542	35.305249401795415	22.54426486290813	14.881372216441918
15-19	21.108847053145155	36.814751235682365	20.25754102055638	21.8188606906161
20-24	26.010140450013775	30.892582718432898	25.97999685814526	17.11727997340807
25-29	19.643832061033944	24.62754222721074	39.14846629677778	16.58015941497754
30-34	19.01837529682559	28.012458137062275	32.189303578299345	20.77986298781279
35-39	19.935296005974077	30.139038455846695	27.369151995191597	22.55651354298763
40-44	19.87368833256305	28.885757152842128	29.362226254522106	21.878328260072717
45-49	22.03974673918735	29.541585179552442	25.76229819844228	22.656369882817927
50-54	21.812759117639693	30.571066249877628	26.943544513023898	20.67263011945878
55-59	21.5929203539823	27.01516969430664	29.553105313604917	21.838804638106144
60-64	22.178261552577116	25.166256480075404	29.387452160708143	23.26802980663933
65-69	19.458872438420784	27.217751023948672	28.401501715498224	24.921874822132317
70-74	20.323474436002922	25.0813353337993	29.75978471464901	24.835405515548768
75-79	19.89495276972709	25.630385833422505	30.405868438247758	24.06879295860265
80-84	23.394478076456352	25.397888582545402	27.024413122024633	24.183220218973613
85-89	21.2189941056073	27.182507609890923	26.04674988787221	25.551748396629563
90-94	19.644879345947803	29.250075700485624	30.027570913710555	21.07747403985602
95-99	22.06192185888519	29.466636007021364	26.854343158838972	21.61709897525448
100-104	21.913799344763916	27.70396442874023	27.026234487092214	23.356001739403638
105-109	23.447980675316632	27.62514485544053	23.321122598359405	25.60575187088343
110-114	20.693074942342413	28.299505271713514	29.429024818375755	21.578394967568318
115-119	24.308894408636913	26.95870737721154	26.597394081929554	22.135004132221997
120-124	17.701938160102543	28.705123272264483	28.05635303519105	25.536585532441926
125-129	19.94276360275117	23.462232856970477	32.31852942984443	24.276474110433917
130-134	25.6798814291341	28.118370515742285	26.041786668063043	20.15996138706057
135-139	24.701261978321202	27.109061063540597	24.116239518613213	24.073437439524987
140-144	20.067117302740474	27.970657808761224	27.734335691242194	24.227889197256115
145-149	21.220861004801574	26.313261131386444	29.78728732716953	22.678590536642453
150-154	23.40185460498007	24.46544073619576	25.219349271795476	26.91335538702869
155-159	21.464605184971006	25.177321272860976	31.346512427401528	22.01156111476649
160-164	24.91754908009681	29.598548372041133	25.495969091434805	19.987933456427257
165-169	20.86774385232372	25.03584674123639	28.314303862887634	25.78210554355225
170-174	22.55523858744032	28.790864032821002	25.784291181633357	22.869606198105323
175-179	19.54502300611751	28.54079060904171	28.856524243507405	23.057662141333378
180-184	19.602532608126474	30.231745937786727	26.465481716909782	23.70023973717702
185-189	21.71044393496816	27.485218484123386	27.4772955460794	23.327042034829056
190-194	20.07772675425915	26.87387729918881	26.347730465290475	26.70066548126157
195-199	20.969330488968218	30.123010443251957	27.15951287591267	21.74814619186715
200-204	22.193469950891444	26.70353413124301	27.048819413930254	24.054176503935288
205-209	20.911411079819047	27.40102588387432	28.477316036436406	23.210246999870225
210-214	23.803602204762413	25.95823154558763	27.8008610503357	22.437305199314256
215-219	23.57743419749517	26.073979295632594	27.822125487499743	22.526461019372494
220-224	19.1553874840346	26.169145620413858	28.922821931967462	25.752644963584082
225-229	22.30657672158841	28.193000949386544	26.302788282247835	23.197634046777207
230-234	21.533999194045958	26.436704148841955	28.594111071395233	23.435185585716855
235-239	23.0706849015666	25.930091455293457	25.1439447579975	25.85527888514244
240-244	22.98726638147125	30.53523089217291	22.98726638147125	23.490236344884583
245-249	21.778016578975528	28.649161830562964	23.895490072422028	25.67733151803948
250-254	22.132054491624903	26.054047211625413	25.923637413405437	25.890260883344247
255-259	22.273291274978316	26.936759928147147	27.112794861929146	23.677153934945395
260-264	21.969942922972194	28.145372252869223	26.097474905004432	23.787209919154158
265-269	21.493246150658766	27.83164211997787	26.482374877911624	24.19273685145174
270-274	20.53611880764336	29.4555256801091	24.656410863531946	25.351944648715595
275-279	21.525211107594867	28.392030617146784	23.708754618867975	26.37400365639037
280-284	19.3581054160567	29.458758603104062	26.38543272218946	24.797703258649776
285-289	21.54847904633325	27.76060888234209	27.8271342414356	22.863777829889056
290-294	21.10980326980564	30.373630276551516	24.74269803360874	23.773868420034105
295-299	20.53825891159777	31.349107872622834	22.680594038216793	25.432039177562604
300-301	16.337530820912	32.16291655188272	29.74232237706355	21.757230250141724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.25
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.0
24	0.25
25	0.5
26	1.25
27	1.5
28	0.0
29	1.5
30	1.75
31	3.5
32	7.0
33	17.0
34	51.75
35	185.25
36	686.75
37	1963.75
38	3989.5
39	5691.0
40	6983.0
41	8700.0
42	12007.25
43	15929.5
44	17076.25
45	15189.0
46	12284.0
47	9951.0
48	8065.0
49	6674.25
50	5488.75
51	4463.0
52	2934.5
53	1530.75
54	712.0
55	409.0
56	347.5
57	375.75
58	414.0
59	485.75
60	624.25
61	747.75
62	731.25
63	610.0
64	430.5
65	254.5
66	145.5
67	70.75
68	39.5
69	21.25
70	16.0
71	11.25
72	9.0
73	10.0
74	7.75
75	5.25
76	3.5
77	3.0
78	4.0
79	2.5
80	2.0
81	2.5
82	2.25
83	1.5
84	1.5
85	1.25
86	0.25
87	0.25
88	1.25
89	0.25
90	0.5
91	1.25
92	1.75
93	0.5
94	0.5
95	0.25
96	0.0
97	0.25
98	0.5
99	0.75
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	4.553412668960069E-5
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	439231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.532465217705328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.8981790248179	7.835050938218243
2	4.7279895429096035	1.2796270807819745
3	3.0838860883252144	1.2519774367687893
4	2.8740415304190967	1.5557146817854806
5	3.1760786404627943	2.1490086865379796
6	3.042013346861456	2.4699563884918816
7	2.7639811659704963	2.6182435293621853
8	2.673553030001118	2.8943810708944655
9	2.3063313581207425	2.8089313995844836
>10	16.65813022029032	39.28073173625394
>50	0.6414527513695376	5.752991309812143
>100	0.10743361961724261	2.369457002846418
>500	0.010428817964540982	1.052812018067151
>1k	0.02433390857996236	8.42014401646647
>5k	0.005214408981420506	4.402946417236601
>10k+	0.0069525453085606745	13.858026286891864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	24845	5.6564768880156455	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	12435	2.831084326925923	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	10932	2.488895364853574	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10705	2.437214131060877	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7744	1.7630813854213385	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	5740	1.3068294359915398	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5235	1.191855766100298	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4863	1.1071622904576406	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4783	1.0889486397818005	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3841	0.8744829030737813	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3709	0.8444303794586447	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	3076	0.7003148684860585	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2493	0.5675828891858725	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2485	0.5657615241182886	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2464	0.5609804408158805	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1834	0.41754794174363835	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1791	0.40775810450537414	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	1305	0.29711017664964445	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1075	0.2447459309566037	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1071	0.24383524842281168	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	1008	0.22949199851558746	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	959	0.2183361374766353	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	872	0.198528792366659	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	710	0.16164614974808245	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	681	0.15504370137809034	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	652	0.14844125300809824	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	602	0.13705772133569807	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	485	0.11042025722228167	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	2.2767063344800344E-4	0.0	0.0
7	2.2767063344800344E-4	0.0	0.0
8	4.5534126689600687E-4	0.0	0.0
9	4.5534126689600687E-4	0.0	0.0
10-14	6.830119003440104E-4	0.0	0.0
15-19	6.830119003440104E-4	0.0	0.0
20-24	7.740801537232117E-4	0.0	0.0
25-29	9.106825337920137E-4	0.0	0.0
30-34	0.003187388868272048	0.0	0.0
35-39	0.003187388868272048	0.0	0.0
40-44	0.005145356315924878	0.0	0.0
45-49	0.00792293804399052	0.0	0.0
50-54	0.010063041998401754	0.0	0.0
55-59	0.012112077699433784	0.0	0.0
60-64	0.015253932441016232	0.0	0.0
65-69	0.021992983191077134	0.0	0.0
70-74	0.03155514979589328	0.0	0.0
75-79	0.04293868146829345	0.0	0.0
80-84	0.05687212423531126	0.0	0.0
85-89	0.07795442489259638	0.0	0.0
90-94	0.11643076194530896	0.0	0.0
95-99	0.15477049661795275	0.0	0.0
100-104	0.19584227889197256	0.0	0.0
105-109	0.24378971429612206	0.0	0.0
110-114	0.306171467860875	0.0	0.0
115-119	0.37766004676354814	0.0	0.0
120-124	0.4552501986426277	0.0	0.0
125-129	0.5462729178951395	0.0	0.0
130-134	0.6546896735430787	0.0	0.0
135-139	0.7687526609005284	0.0	0.0
140-144	0.910090590145049	0.0	0.0
145-149	1.1045668452363333	0.0	0.0
150-154	1.3073303113851253	0.0	0.0
155-159	1.5596804414988923	0.0	0.0
160-164	1.8106645478119716	0.0	0.0
165-169	2.1012633443450026	0.0	0.0
170-174	2.429109056510128	0.0	0.0
175-179	2.788191179584319	0.0	0.0
180-184	3.1928529634748	0.0	0.0
185-189	3.6744218873440166	0.0	0.0
190-194	4.22256170443343	0.0	0.0
195-199	4.748981743091903	0.0	0.0
200-204	5.269664481787488	0.0	9.106825337920137E-5
205-209	5.805009209277122	0.0	2.2767063344800344E-4
210-214	6.3596148723564605	0.0	2.2767063344800344E-4
215-219	6.926378147261919	0.0	2.2767063344800344E-4
220-224	7.503933010192813	0.0	2.2767063344800344E-4
225-229	8.066825884329656	0.0	2.2767063344800344E-4
230-234	8.593746798381718	0.0	2.2767063344800344E-4
235-239	9.070944446088731	0.0	2.2767063344800344E-4
240-244	9.507343516281866	0.0	2.2767063344800344E-4
245-249	9.826719880882724	0.0	2.2767063344800344E-4
250-254	10.137490295539248	0.0	2.2767063344800344E-4
255-259	10.38574235425095	0.0	2.2767063344800344E-4
260-264	10.609679189310409	0.0	2.2767063344800344E-4
265-269	10.772327089845662	0.0	2.2767063344800344E-4
270-274	10.903419840585022	0.0	2.2767063344800344E-4
275-279	10.998130824099391	0.0	2.2767063344800344E-4
280-284	11.068435515708135	0.0	2.2767063344800344E-4
285-289	11.123213070115726	0.0	2.2767063344800344E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8140	0.0	290.6511	1
GGGTTAT	8230	0.0	288.36874	2
GTTATTA	8260	0.0	286.4286	4
GGTTATT	8345	0.0	284.04135	3
TTATTAG	8425	0.0	279.24332	5
TTAGTAG	8530	0.0	278.22687	8
TAGTAGA	8640	0.0	274.6846	9
GGGGGGA	6365	0.0	273.6803	1
TATTAGT	8660	0.0	272.1767	6
GGGGAGC	6505	0.0	269.8309	3
GGAGCGA	4870	0.0	268.95276	5
ATTAGTA	8815	0.0	266.88882	7
GGGAGCG	4985	0.0	264.52356	4
GCGAAAG	5065	0.0	260.63672	8
CGAAAGC	5075	0.0	260.4138	9
GAGCGAA	5230	0.0	250.15775	6
AGCGAAA	5305	0.0	246.06503	7
GGGGGAG	7160	0.0	245.55864	2
GGGAGCA	2075	0.0	207.56627	4
CAAAAGC	2445	0.0	156.24744	9
>>END_MODULE