##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6617_flu.3520000003bae0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453311
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.20323795363448	34.0	34.0	34.0	34.0	34.0
2	33.4116820461008	34.0	34.0	34.0	34.0	34.0
3	33.43686343371328	34.0	34.0	34.0	34.0	34.0
4	33.428315218470324	34.0	34.0	34.0	34.0	34.0
5	33.36156634187125	34.0	34.0	34.0	34.0	34.0
6	36.8644462631615	38.0	38.0	38.0	36.0	38.0
7	36.141679773930036	38.0	38.0	38.0	34.0	38.0
8	36.80461758042492	38.0	38.0	38.0	35.0	38.0
9	36.861289489996935	38.0	38.0	38.0	36.0	38.0
10-14	36.873160368929945	38.0	38.0	38.0	36.0	38.0
15-19	36.77003470023891	38.0	38.0	38.0	35.6	38.0
20-24	36.85029394830481	38.0	38.0	38.0	35.8	38.0
25-29	36.75991670177869	38.0	38.0	38.0	35.8	38.0
30-34	36.42139458340962	38.0	37.6	38.0	33.4	38.0
35-39	36.67066848146195	38.0	38.0	38.0	34.8	38.0
40-44	36.204995687287536	38.0	37.2	38.0	33.2	38.0
45-49	36.51461402877936	38.0	37.4	38.0	34.2	38.0
50-54	36.4710412939461	38.0	37.6	38.0	34.0	38.0
55-59	36.093498282635984	38.0	37.0	38.0	33.0	38.0
60-64	36.28489359402265	38.0	37.0	38.0	34.0	38.0
65-69	36.14227230312081	38.0	37.0	38.0	33.0	38.0
70-74	35.98614064075215	38.0	37.0	38.0	32.0	38.0
75-79	36.037579939599965	38.0	37.0	38.0	32.0	38.0
80-84	35.32343975769395	38.0	36.6	38.0	28.8	38.0
85-89	35.52449907458676	38.0	37.0	38.0	30.0	38.0
90-94	35.50779222211683	38.0	37.0	38.0	30.0	38.0
95-99	34.576771796845875	38.0	35.2	38.0	24.8	38.0
100-104	34.88685074926485	38.0	36.0	38.0	25.6	38.0
105-109	34.67278446805835	38.0	35.2	38.0	24.6	38.0
110-114	34.19238491896292	37.8	34.2	38.0	24.0	38.0
115-119	34.21378567914743	37.6	34.4	38.0	23.6	38.0
120-124	33.70706203908575	37.0	33.2	38.0	22.4	38.0
125-129	33.253549108669326	37.0	32.0	38.0	22.4	38.0
130-134	33.02580965385795	37.0	31.6	38.0	21.6	38.0
135-139	32.35659889126891	36.8	30.0	38.0	19.2	38.0
140-144	31.130887183412717	35.4	26.6	38.0	15.4	38.0
145-149	30.87703982475607	35.0	26.8	37.6	15.0	38.0
150-154	29.869337386474182	35.0	23.8	37.0	10.4	38.0
155-159	29.661642448561803	34.8	24.0	37.0	10.0	37.4
160-164	28.950005184078925	33.6	22.8	37.0	10.2	37.4
165-169	27.794927544224606	31.8	21.0	36.6	9.6	37.0
170-174	27.22806373549285	30.2	20.4	36.0	9.2	37.0
175-179	26.340107343523545	28.6	19.8	35.4	9.0	37.0
180-184	25.48416208739695	27.2	18.0	35.0	9.0	36.8
185-189	24.764375450849418	26.0	16.6	35.0	8.4	36.2
190-194	23.793341877871928	24.8	15.2	34.0	8.0	36.0
195-199	22.960380842291496	23.6	14.2	33.0	8.0	36.0
200-204	22.110638832942506	23.0	12.4	31.4	8.0	35.6
205-209	21.22455135657418	21.8	9.4	30.0	8.0	35.0
210-214	19.976014700724228	20.2	9.0	28.2	8.0	34.4
215-219	19.426470568770668	19.2	9.0	27.0	8.0	34.0
220-224	18.760540114843895	18.2	9.0	26.0	8.0	33.2
225-229	17.989684344743456	16.6	9.0	25.2	8.0	32.0
230-234	17.116432206586648	15.4	9.0	24.0	7.6	30.8
235-239	16.09504071156447	14.4	8.2	22.4	7.0	29.0
240-244	15.410313890463721	13.2	8.0	21.0	7.0	28.0
245-249	14.757272600929605	12.6	8.0	20.2	7.0	26.6
250-254	14.085220521893358	11.6	8.0	19.0	7.0	25.6
255-259	13.47709673932466	11.0	8.0	18.0	7.0	24.2
260-264	12.947039008539388	10.2	8.0	16.4	7.0	23.2
265-269	12.540213231093002	9.0	8.0	15.8	7.0	22.4
270-274	12.078006931223817	9.0	7.4	14.4	7.0	21.2
275-279	11.67248577687283	8.2	7.0	14.0	7.0	20.6
280-284	11.511579026319678	8.0	7.0	13.4	7.0	20.2
285-289	11.218830780634047	8.0	7.0	13.0	7.0	19.0
290-294	10.966484378274519	8.0	7.0	13.0	7.0	19.0
295-299	10.685524066259147	8.0	7.0	13.0	7.0	18.4
300-301	10.40588580466832	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.14576349718470283
1101	2	0.03518798926751998
1101	3	0.12721714514786697
1101	4	0.11261484189994775
1101	5	0.23700710731306174
1101	6	0.26066401806782125
1101	7	0.916328078925801
1101	8	0.37263073506171907
1101	9	0.22892413099797437
1101	10-14	0.13159891910137844
1101	15-19	0.13525018518463128
1101	20-24	0.16354232390893486
1101	25-29	0.09126107982695686
1101	30-34	0.49776332761638287
1101	35-39	0.2543002124858589
1101	40-44	0.5165134509297431
1101	45-49	0.22111917706040884
1101	50-54	0.20549630223974447
1101	55-59	0.2902658192083294
1101	60-64	0.33343686424309027
1101	65-69	0.3788346186052678
1101	70-74	0.24419743634793178
1101	75-79	0.3613573917151953
1101	80-84	0.7406652444640756
1101	85-89	0.3795544227420109
1101	90-94	0.3429155164889224
1101	95-99	0.9309312369510394
1101	100-104	0.4945992375081829
1101	105-109	0.6338213619479802
1101	110-114	0.6481701470925856
1101	115-119	0.613821730156495
1101	120-124	0.9070354373230387
1101	125-129	0.7160178451812342
1101	130-134	1.017511571363407
1101	135-139	0.6232958893377258
1101	140-144	1.1708925730580013
1101	145-149	1.3992321338002043
1101	150-154	1.390468285162438
1101	155-159	0.9150491625250581
1101	160-164	1.3900325505393383
1101	165-169	0.8534863958011272
1101	170-174	0.969638736045674
1101	175-179	1.1184372648988194
1101	180-184	0.8503077971610935
1101	185-189	0.7823216495889405
1101	190-194	0.9705690034610264
1101	195-199	1.0742277037815633
1101	200-204	1.1117685468408176
1101	205-209	1.0871555284970569
1101	210-214	1.0222224255451557
1101	215-219	1.179145689269312
1101	220-224	0.7879726717259743
1101	225-229	0.9083100645422171
1101	230-234	0.7660700603150623
1101	235-239	0.4501633594060195
1101	240-244	0.2826715471621277
1101	245-249	0.2148289148626059
1101	250-254	0.45019685546873767
1101	255-259	0.11954605500850057
1101	260-264	0.16559159486218533
1101	265-269	0.05815781243875584
1101	270-274	0.07253020093542162
1101	275-279	0.2309649761938033
1101	280-284	0.12158329344611474
1101	285-289	0.0989968048862142
1101	290-294	-0.1051204505608272
1101	295-299	-0.09161572858226386
1101	300-301	-0.16685262808099033
1102	1	0.10086134045377548
1102	2	-0.07001178542950015
1102	3	-0.03308374334532971
1102	4	0.05302729170644227
1102	5	0.11442048376889602
1102	6	0.14246208118899517
1102	7	0.9258700234358628
1102	8	0.2084161158055764
1102	9	0.23780397546623533
1102	10-14	0.18836771100500727
1102	15-19	0.23696837476619237
1102	20-24	0.12849244837065754
1102	25-29	0.13079375784356273
1102	30-34	0.4203643414797895
1102	35-39	0.13097212410893633
1102	40-44	0.5126651307087968
1102	45-49	0.22198463120943757
1102	50-54	0.1820349943922963
1102	55-59	0.40696476718877506
1102	60-64	0.2549500281146635
1102	65-69	0.20711304947904097
1102	70-74	0.23616385893637215
1102	75-79	0.34005986459707316
1102	80-84	0.8143555982260864
1102	85-89	0.37298560540095593
1102	90-94	0.31238594547306064
1102	95-99	1.1185831801892263
1102	100-104	0.7020194093925554
1102	105-109	0.8247218834153571
1102	110-114	0.8377338224382598
1102	115-119	0.6270531199488047
1102	120-124	1.079521873720374
1102	125-129	1.0477325825397443
1102	130-134	0.9758478178884786
1102	135-139	0.9729963453945629
1102	140-144	1.9904750357649128
1102	145-149	1.7449908052394179
1102	150-154	1.6148954886800695
1102	155-159	1.2388875984863184
1102	160-164	1.5176980846345849
1102	165-169	1.2504448055981712
1102	170-174	1.58883454977099
1102	175-179	1.2648830377425426
1102	180-184	1.5932629727702299
1102	185-189	1.5019877651112274
1102	190-194	1.6138437459070225
1102	195-199	1.5535657490965349
1102	200-204	1.6970801008862786
1102	205-209	1.3283486167831207
1102	210-214	1.505897625864943
1102	215-219	1.5269065047207313
1102	220-224	1.5118070376979595
1102	225-229	1.26717032218707
1102	230-234	1.321783489462728
1102	235-239	0.6898472084530276
1102	240-244	0.7808395432374926
1102	245-249	0.5607777129438958
1102	250-254	0.8863405406259979
1102	255-259	0.6987004420572127
1102	260-264	0.6025037812095491
1102	265-269	0.4505370482255895
1102	270-274	0.21316969800103358
1102	275-279	0.16913718761520968
1102	280-284	0.4247999658664856
1102	285-289	0.2793720433621498
1102	290-294	0.061592851399671744
1102	295-299	0.0962235795195614
1102	300-301	4.940463808722484E-6
1103	1	-0.21240612311257934
1103	2	-0.0604936110949339
1103	3	0.06799676422536294
1103	4	0.08512568930441233
1103	5	-0.043244517619413614
1103	6	0.09462501968840087
1103	7	0.9703849210677546
1103	8	0.26607559191082686
1103	9	0.1735757071389088
1103	10-14	-0.027186450407398866
1103	15-19	0.05409703203147842
1103	20-24	0.1281607915061258
1103	25-29	0.017578189973853853
1103	30-34	0.5247030874923269
1103	35-39	0.21850799473618565
1103	40-44	0.4152222177236169
1103	45-49	0.14277794995322068
1103	50-54	0.08661153739753757
1103	55-59	0.26157918286212123
1103	60-64	0.18973742011811368
1103	65-69	0.3060160359994484
1103	70-74	0.08972091727567033
1103	75-79	0.2744639838005156
1103	80-84	0.7744480872681905
1103	85-89	0.4566707034327564
1103	90-94	0.4193499702850829
1103	95-99	1.0964391045865653
1103	100-104	0.5842603802757083
1103	105-109	0.9442538989337095
1103	110-114	0.5747391609807551
1103	115-119	0.7868925650145613
1103	120-124	1.2461111164838243
1103	125-129	0.9047676317182507
1103	130-134	1.1752224117976553
1103	135-139	1.1706823008943488
1103	140-144	1.612901390385975
1103	145-149	1.6764154680048193
1103	150-154	2.256030172000923
1103	155-159	0.8894387629572158
1103	160-164	1.1483389265478436
1103	165-169	1.6310945648986532
1103	170-174	2.0811043990007008
1103	175-179	2.1312857497473026
1103	180-184	1.6190990565055472
1103	185-189	1.5512948316685033
1103	190-194	1.5827235332325778
1103	195-199	1.7793826691918504
1103	200-204	2.005218143439347
1103	205-209	1.525954280827726
1103	210-214	2.3123026179232227
1103	215-219	2.1860899797880684
1103	220-224	1.5447167426828123
1103	225-229	0.9659745530152115
1103	230-234	1.7394403358130006
1103	235-239	1.2519630701419047
1103	240-244	1.1047196068272047
1103	245-249	1.2831995214459795
1103	250-254	0.8219995285480053
1103	255-259	0.9229182473168667
1103	260-264	0.8284270105486637
1103	265-269	0.46587691258338637
1103	270-274	0.48053856519910454
1103	275-279	0.5236790752057736
1103	280-284	0.4914708289506695
1103	285-289	0.3341948811055673
1103	290-294	0.12031388125648057
1103	295-299	0.12268562886803025
1103	300-301	-0.18711261727714934
2116	1	0.07972123151478172
2116	2	0.030901761550438778
2116	3	-0.025778829550233695
2116	4	2.823232110458207E-4
2116	5	-0.004137228512128388
2116	6	0.045024282587661446
2116	7	0.7901535533363813
2116	8	0.055413224998460464
2116	9	0.20536317642638124
2116	10-14	0.14057562985468763
2116	15-19	0.24255423923869301
2116	20-24	0.21375149584682163
2116	25-29	0.20877904992471485
2116	30-34	0.5958435461327767
2116	35-39	0.020422409150604892
2116	40-44	0.4317916280430296
2116	45-49	0.3106628803381497
2116	50-54	0.23882795836395587
2116	55-59	0.41178393306687155
2116	60-64	0.3276878705685604
2116	65-69	0.5347834397668549
2116	70-74	0.5058245208792798
2116	75-79	0.5186746717612039
2116	80-84	0.9522736400937433
2116	85-89	0.543622996635051
2116	90-94	0.6270690530041705
2116	95-99	1.3505545837020492
2116	100-104	0.5861952697106005
2116	105-109	0.682659918589728
2116	110-114	0.8515460815376699
2116	115-119	1.017190476562476
2116	120-124	1.18392847930118
2116	125-129	1.1213933483439718
2116	130-134	1.139575401150644
2116	135-139	1.5422373786994257
2116	140-144	2.2453901291247114
2116	145-149	2.0356445834839363
2116	150-154	2.380451752672503
2116	155-159	2.08533352135197
2116	160-164	1.9879069036158192
2116	165-169	1.8685740898781766
2116	170-174	1.8443210247173312
2116	175-179	2.113329156790712
2116	180-184	1.9427477051541935
2116	185-189	1.6645933574670302
2116	190-194	1.778577341586388
2116	195-199	1.094636559445739
2116	200-204	0.9342773162485223
2116	205-209	1.0491627165361592
2116	210-214	2.187962937333541
2116	215-219	2.0353426415407334
2116	220-224	1.2508336550497248
2116	225-229	1.0452128822535443
2116	230-234	1.2054569205795822
2116	235-239	0.8134597941386268
2116	240-244	1.2691596150172177
2116	245-249	0.9666966549431102
2116	250-254	1.1434573500058267
2116	255-259	1.7006465437951643
2116	260-264	1.0161368795085348
2116	265-269	0.8955554547619275
2116	270-274	0.587986353896893
2116	275-279	0.5949014339281327
2116	280-284	0.2653754153552583
2116	285-289	0.2534362815969686
2116	290-294	0.36540499509759705
2116	295-299	0.23143358136598202
2116	300-301	0.010932018267487464
1117	1	0.124203213496763
1117	2	0.13242203182070966
1117	3	0.18196891819751215
1117	4	0.1277034943822173
1117	5	0.16257448319958456
1117	6	0.10844770601108422
1117	7	0.9562571569399907
1117	8	0.36562718896242785
1117	9	0.2485163295795303
1117	10-14	0.20820175598080937
1117	15-19	0.2079168518513015
1117	20-24	0.2617267210720513
1117	25-29	0.287402923798588
1117	30-34	0.523149852439083
1117	35-39	0.2654314181596149
1117	40-44	0.5009425289439164
1117	45-49	0.13009080826608255
1117	50-54	0.28479417458017764
1117	55-59	0.3804821312650759
1117	60-64	0.35379147417216217
1117	65-69	0.4354906469740598
1117	70-74	0.4475272235819716
1117	75-79	0.5302084555449866
1117	80-84	0.9204808473009507
1117	85-89	0.5142423660044244
1117	90-94	0.3651140980492116
1117	95-99	1.1181581873056459
1117	100-104	0.6160105850259185
1117	105-109	0.5070270357068409
1117	110-114	0.7345708563124518
1117	115-119	0.7813323684543647
1117	120-124	0.912631182003885
1117	125-129	0.7891951501457797
1117	130-134	0.8427420678173121
1117	135-139	1.0123207120327606
1117	140-144	1.2317932822778666
1117	145-149	1.7325477366370876
1117	150-154	2.191904455375205
1117	155-159	1.651847034865483
1117	160-164	1.7252807774896972
1117	165-169	1.2879119277160171
1117	170-174	1.1568976012939025
1117	175-179	1.309437264898822
1117	180-184	1.3093468042532876
1117	185-189	1.5660663304399982
1117	190-194	-0.6594451809361352
1117	195-199	1.1105149378241173
1117	200-204	1.7516444333656374
1117	205-209	1.5345455994190473
1117	210-214	2.1213075319281387
1117	215-219	1.6386669658650561
1117	220-224	1.8457953667614397
1117	225-229	1.4471363056769633
1117	230-234	1.192297010669673
1117	235-239	0.9262165508953828
1117	240-244	0.9819906960982951
1117	245-249	0.9661516098980663
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2108	240-244	-0.5658453694754737
2108	245-249	-0.6682391004844455
2108	250-254	-0.5833259907119412
2108	255-259	-0.4718351797316451
2108	260-264	-0.3493034173456966
2108	265-269	-0.39447002153403865
2108	270-274	-0.45755141748104045
2108	275-279	-0.24337614693141596
2108	280-284	-0.6296877215521413
2108	285-289	-0.9097886851730799
2108	290-294	-0.8012592716281475
2108	295-299	-0.6379666180137242
2108	300-301	2.629856475511616
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	27.0
11	247.0
12	598.0
13	797.0
14	1183.0
15	1946.0
16	2839.0
17	4119.0
18	5177.0
19	6128.0
20	8011.0
21	10318.0
22	14010.0
23	18663.0
24	25696.0
25	35726.0
26	49382.0
27	63490.0
28	72164.0
29	65625.0
30	43616.0
31	18749.0
32	4346.0
33	432.0
34	11.0
35	5.0
36	4.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.496723000324284	19.993779105294156	14.553584625124913	34.95591326925665
2	48.38554546437214	23.055915254648575	14.845437238452188	13.713102042527096
3	48.0167037640825	22.305216506989684	16.863698432202177	12.814381296725646
4	48.29355563840277	20.88169049504645	17.320559174606394	13.504194691944383
5	29.50314464021389	21.047801619638616	36.2413442426943	13.207709497453182
6	28.421547237988932	20.710064392878177	36.43635384978525	14.432034519347644
7	16.07924802177754	50.75257384003476	18.875782851066926	14.292395287120762
8	27.790633803282955	19.09660255321402	38.12459878538134	14.988164858121685
9	14.551378634094474	18.044124232590868	38.9933180531688	28.41117908014586
10-14	26.99299156649629	35.249927753793756	22.643439051776816	15.113641627933141
15-19	21.314726534321913	36.56637496111941	20.34841422334777	21.770484281210916
20-24	25.975191424871667	30.553990527474518	26.142096706234792	17.328721341419026
25-29	19.33295243221541	25.21986009604885	38.452188453401746	16.994999018333992
30-34	18.911078707553976	27.865505138856108	31.84374524333184	21.379670910258078
35-39	20.429418213985542	30.402130104938998	27.887918007725382	21.280533673350085
40-44	20.457743138816397	29.056166737626043	28.799389381682772	21.686700741874784
45-49	21.140409123096504	30.229356887434893	26.32739995279179	22.302834036676806
50-54	21.950890227680333	29.654740343825765	27.483383372563207	20.910986055930696
55-59	21.87354818215309	26.561146762377263	29.91350309169643	21.651801963773217
60-64	21.736732618445174	26.06991667971878	28.723547410056234	23.469803291779815
65-69	20.129579913128072	27.359318437011236	28.564451336940866	23.94665031291983
70-74	20.641634551113917	25.766416433750784	29.584236870492887	24.007712144642422
75-79	20.13160942487608	25.374058869076638	30.641391892100568	23.852939813946715
80-84	23.85951366721743	25.391883276602595	27.03869970064702	23.70990335553296
85-89	21.120864042566804	28.63901383376975	25.983618310607948	24.256503813055495
90-94	20.06375314077973	30.22547434322132	29.10434558173087	20.606426934268086
95-99	21.833707984143334	29.798923917575348	26.691344352993863	21.676023745287452
100-104	22.394603263543132	28.53722940762523	26.214673811136286	22.853493517695355
105-109	23.53510062628085	28.057558717966252	23.624972700861	24.782367954891896
110-114	20.979636496798005	28.110414263055606	29.516910024243842	21.393039215902547
115-119	23.670989673755987	27.077833981527032	26.729993315847178	22.521183028869803
120-124	18.912490541813458	28.241273650981334	27.741793161869005	25.104442645336206
125-129	20.47177324177	24.315536133030083	31.57505553582419	23.637635089375728
130-134	25.050881183117106	28.219301980318146	25.794697238761028	20.935119597803713
135-139	24.500618780484036	26.712345387603655	24.782103235968243	24.004932595944066
140-144	20.686151450108202	28.788138827427527	26.91547304168661	23.610236680777653
145-149	20.701990465706764	27.048759019745827	29.057313852961876	23.191936661585533
150-154	22.807741263724022	25.165769195982453	25.824081039286494	26.202408501007035
155-159	21.78751453196591	26.261264341699185	29.287751676001683	22.663469450333213
160-164	24.764808266289588	28.91048308997576	25.769195982449133	20.55551266128552
165-169	20.42280024089422	25.182137649428316	28.41682641718379	25.978235692493673
170-174	21.97100886587795	28.47457926236072	26.357401430805783	23.197010440955548
175-179	20.422667881432393	27.939449958196473	28.708193712484366	22.92968844788677
180-184	20.07037111387105	29.051004718614813	26.551749240587586	24.326874926926546
185-189	22.06811659103794	26.987697188023237	27.509193467619358	23.434992753319463
190-194	20.259998102847714	27.241474396165106	27.000844894564658	25.497682606422522
195-199	21.224104422791417	29.452539205975587	27.130866005898817	22.192490365334177
200-204	22.964807825091384	26.448111781977495	27.320051796669393	23.267028596261728
205-209	21.745644822208153	26.906913796488503	27.460441065846624	23.887000315456717
210-214	23.671430871962073	26.06184275254737	27.640008735724482	22.626717639766078
215-219	23.904957082444504	26.007354774095486	27.848474888101105	22.2392132553589
220-224	19.96258639212373	26.372887487839474	28.45110751779683	25.213418602239962
225-229	22.792431685972765	27.836518416716117	25.832596164663997	23.538453732647124
230-234	22.33010008581305	26.3770347509767	28.122679573184854	23.17018559002539
235-239	22.822742002731015	26.266823439095898	25.74501832075551	25.165416237417578
240-244	23.287147234459344	29.87000094857614	23.239233109278178	23.603618707686337
245-249	21.605079073748488	28.460681518868945	24.272166349371624	25.662073058010947
250-254	22.293789473452	26.521791882394208	25.009937989592135	26.17448065456166
255-259	22.364690025170358	26.596398499043705	26.813776855183306	24.22513462060263
260-264	21.645889907811636	27.89784496736236	26.152773702822124	24.30349142200388
265-269	21.054860790936026	28.09497232584252	25.830566652915987	25.01960023030546
270-274	20.264189485805552	29.762878024137958	24.279181400848422	25.69375108920807
275-279	21.157439373851506	28.77874130563785	23.133169060534602	26.93065025997604
280-284	18.958948712914534	29.68778608946176	26.286677358369857	25.066587839253845
285-289	20.314530201120203	28.400502083558525	26.346283236012365	24.938684479308908
290-294	19.785930630406057	30.565858759218283	24.25923924193324	25.388971368442416
295-299	19.133442603422374	31.687119880170567	22.290965804933037	26.888471711474022
300-301	14.765470063598721	32.519727074789714	29.813858476851436	22.900944384760134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.25
21	0.25
22	0.0
23	0.25
24	0.25
25	1.0
26	2.25
27	2.0
28	2.0
29	2.0
30	5.0
31	4.0
32	8.5
33	17.75
34	60.25
35	180.5
36	612.0
37	1755.25
38	3567.5
39	5284.75
40	6875.5
41	8983.5
42	12077.5
43	15527.75
44	17551.0
45	16561.0
46	13430.75
47	10693.25
48	8766.5
49	7420.0
50	6132.0
51	4693.0
52	2970.75
53	1654.75
54	992.0
55	660.0
56	564.0
57	531.5
58	468.25
59	479.75
60	512.25
61	522.0
62	485.25
63	393.25
64	260.5
65	145.75
66	85.75
67	50.0
68	27.75
69	18.0
70	13.0
71	7.25
72	6.25
73	6.5
74	4.0
75	3.5
76	3.0
77	3.75
78	2.75
79	1.75
80	1.5
81	1.0
82	1.5
83	2.0
84	1.5
85	1.0
86	1.25
87	0.5
88	0.75
89	0.25
90	0.25
91	0.5
92	1.5
93	0.75
94	0.25
95	0.5
96	0.75
97	0.5
98	0.75
99	0.25
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	453311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.984246284166554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.23844923571018	8.284050636042691
2	4.3702873563933835	1.2223034944876847
3	3.1926338936195355	1.3393973599065956
4	2.54133134167102	1.4215441348639586
5	2.6975296634788983	1.886145958646692
6	2.4331213119282977	2.0415220599155846
7	2.444766441145313	2.393175121416901
8	2.317275199284484	2.5924277675988283
9	2.0781175024262533	2.6154816265229224
>10	18.18988748880614	45.194898300235344
>50	0.3869320749501542	3.43177952283944
>100	0.0640949394596094	1.8528675766702287
>500	0.011393387841350246	1.0602136444296362
>1k	0.021159148700495338	6.992675695976618
>5k	0.009765760938690147	10.604412495135424
>10k+	0.0032552536462300487	7.067104605311434
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	18111	3.995270355230736	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	12938	2.8541111951838802	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	9724	2.1451056780003133	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	9231	2.036350320199598	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	8964	1.9774503596868376	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	6538	1.4422769357019793	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	6188	1.365067249636563	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5945	1.3114616675968596	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4922	1.0857887851827994	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4140	0.9132802866023547	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3996	0.8815140157640119	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3187	0.7030493414013779	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2845	0.6276044481603138	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2476	0.5462033791370604	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1835	0.40479935408582635	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1477	0.32582487519605746	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1382	0.304867960406873	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1217	0.2684691084046052	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1153	0.2543507658097862	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1085	0.239350026802791	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1007	0.22214329676535532	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	759	0.16743471921043168	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT	757	0.1669935210043436	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	743	0.16390513356172692	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	691	0.1524339802034365	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA	581	0.1281680788685913	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC	579	0.1277268806625032	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	548	0.12088830846813776	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	471	0.10390217753374616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.2059910304404702E-4	0.0	0.0
2	2.2059910304404702E-4	0.0	0.0
3	4.4119820608809403E-4	0.0	0.0
4	4.4119820608809403E-4	0.0	0.0
5	4.4119820608809403E-4	0.0	0.0
6	4.4119820608809403E-4	0.0	0.0
7	4.4119820608809403E-4	0.0	0.0
8	6.617973091321411E-4	0.0	0.0
9	6.617973091321411E-4	0.0	0.0
10-14	0.0015883135419171384	0.0	0.0
15-19	0.0022059910304404705	0.0	0.0
20-24	0.0024265901334845174	0.0	0.0
25-29	0.0028677883395726114	0.0	0.0
30-34	0.004676700984533797	0.0	0.0
35-39	0.005382618114274747	0.0	0.0
40-44	0.005956175782189269	0.0	0.0
45-49	0.00666209291193022	0.0	0.0
50-54	0.01036815784307021	0.0	0.0
55-59	0.013765384029948532	0.0	0.0
60-64	0.016809651651956383	0.0	0.0
65-69	0.022501108510492796	0.0	0.0
70-74	0.03229570868564848	0.0	0.0
75-79	0.04407570078820059	0.0	0.0
80-84	0.060752992978330554	0.0	0.0
85-89	0.08104811045838287	0.0	0.0
90-94	0.10840239923584472	0.0	0.0
95-99	0.1435658962610658	0.0	0.0
100-104	0.19037702592701256	0.0	0.0
105-109	0.24649743774141814	0.0	0.0
110-114	0.3153684777117697	0.0	0.0
115-119	0.3942988367809297	0.0	0.0
120-124	0.4873034186242998	0.0	0.0
125-129	0.6006472377683313	0.0	0.0
130-134	0.7432424979760033	0.0	0.0
135-139	0.9067505531522508	0.0	0.0
140-144	1.1064809810483311	0.0	0.0
145-149	1.3756560065826773	0.0	0.0
150-154	1.7028927160382168	0.0	0.0
155-159	2.1043830835783823	0.0	0.0
160-164	2.5421840634795982	0.0	0.0
165-169	3.0706512747319166	0.0	0.0
170-174	3.6480914868600136	0.0	0.0
175-179	4.309182878862414	0.0	0.0
180-184	5.053748971456683	0.0	0.0
185-189	5.934336470987909	0.0	0.0
190-194	6.793614097165081	0.0	0.0
195-199	7.671466167818562	0.0	0.0
200-204	8.496683292485733	0.0	0.0
205-209	9.325121164057347	0.0	0.0
210-214	10.090423572337755	0.0	0.0
215-219	10.835916181164809	1.764792824352376E-4	0.0
220-224	11.594115298327198	2.2059910304404702E-4	0.0
225-229	12.28397281336654	2.2059910304404702E-4	0.0
230-234	12.896002964851945	2.2059910304404702E-4	0.0
235-239	13.407969363196568	2.2059910304404702E-4	0.0
240-244	13.888301850164675	2.2059910304404702E-4	0.0
245-249	14.229524542753207	2.2059910304404702E-4	0.0
250-254	14.537172051858438	2.2059910304404702E-4	0.0
255-259	14.770080584852343	2.2059910304404702E-4	0.0
260-264	14.969546293824768	2.2059910304404702E-4	0.0
265-269	15.119906642459592	2.2059910304404702E-4	0.0
270-274	15.244015697832172	2.2059910304404702E-4	0.0
275-279	15.329475790351438	2.2059910304404702E-4	0.0
280-284	15.389125787814546	2.2059910304404702E-4	0.0
285-289	15.427995349770907	2.2059910304404702E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8270	0.0	292.32468	1
GGGTTAT	8350	0.0	289.17065	2
GTTATTA	8390	0.0	286.56137	4
GGTTATT	8505	0.0	283.55377	3
TTATTAG	8530	0.0	279.95605	5
TTAGTAG	8680	0.0	277.83698	8
GGGGGGA	5835	0.0	274.77722	1
GGGGAGC	5875	0.0	271.90213	3
TATTAGT	8815	0.0	271.574	6
ATTAGTA	8855	0.0	270.34726	7
GGAGCGA	4580	0.0	269.2358	5
TAGTAGA	8995	0.0	267.9433	9
GGGAGCG	4665	0.0	265.2787	4
CGAAAGC	4835	0.0	256.8666	9
GAGCGAA	4865	0.0	252.25078	6
AGCGAAA	4980	0.0	246.12953	7
GCGAAAG	5110	0.0	243.33171	8
GGGGGAG	6865	0.0	233.98032	2
GGGAGCA	1790	0.0	196.94135	4
CAAAAGC	2305	0.0	138.22127	9
>>END_MODULE