FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6617_flu.3520000003bae0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6617_flu.3520000003bae0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453311
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC181113.995270355230736No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT129382.8541111951838802No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA97242.1451056780003133No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA92312.036350320199598No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG89641.9774503596868376No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT65381.4422769357019793No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT61881.365067249636563No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG59451.3114616675968596No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA49221.0857887851827994No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT41400.9132802866023547No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG39960.8815140157640119No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA31870.7030493414013779No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT28450.6276044481603138No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA24760.5462033791370604No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG18350.40479935408582635No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14770.32582487519605746No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG13820.304867960406873No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC12170.2684691084046052No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11530.2543507658097862No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10850.239350026802791No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10070.22214329676535532No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7590.16743471921043168No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT7570.1669935210043436Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC7430.16390513356172692No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA6910.1524339802034365No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA5810.1281680788685913No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC5790.1277268806625032No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5480.12088830846813776No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT4710.10390217753374616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA82700.0292.324681
GGGTTAT83500.0289.170652
GTTATTA83900.0286.561374
GGTTATT85050.0283.553773
TTATTAG85300.0279.956055
TTAGTAG86800.0277.836988
GGGGGGA58350.0274.777221
GGGGAGC58750.0271.902133
TATTAGT88150.0271.5746
ATTAGTA88550.0270.347267
GGAGCGA45800.0269.23585
TAGTAGA89950.0267.94339
GGGAGCG46650.0265.27874
CGAAAGC48350.0256.86669
GAGCGAA48650.0252.250786
AGCGAAA49800.0246.129537
GCGAAAG51100.0243.331718
GGGGGAG68650.0233.980322
GGGAGCA17900.0196.941354
CAAAAGC23050.0138.221279
Produced by FastQC (version 0.11.2)