##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6471_flu.3520000003bb5c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294938
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.962069994371696	34.0	34.0	34.0	33.0	34.0
2	33.34522509815622	34.0	34.0	34.0	34.0	34.0
3	33.36297120072693	34.0	34.0	34.0	34.0	34.0
4	33.30177189782259	34.0	34.0	34.0	34.0	34.0
5	33.3046606405414	34.0	34.0	34.0	34.0	34.0
6	36.84756457289328	38.0	38.0	38.0	36.0	38.0
7	35.93823786694153	38.0	38.0	38.0	34.0	38.0
8	36.495948300998855	38.0	37.0	38.0	34.0	38.0
9	36.75214790905207	38.0	38.0	38.0	36.0	38.0
10-14	36.6993964833287	38.0	38.0	38.0	35.4	38.0
15-19	36.66401480989225	38.0	38.0	38.0	35.4	38.0
20-24	36.78038909872583	38.0	38.0	38.0	35.8	38.0
25-29	36.71901145325458	38.0	38.0	38.0	35.6	38.0
30-34	36.37813777810929	38.0	37.6	38.0	33.4	38.0
35-39	36.64625717947501	38.0	38.0	38.0	34.8	38.0
40-44	36.144835185700046	38.0	37.2	38.0	33.0	38.0
45-49	36.49164705802575	38.0	37.8	38.0	34.0	38.0
50-54	36.416012178830805	38.0	37.6	38.0	34.0	38.0
55-59	36.04765137079657	38.0	37.0	38.0	32.8	38.0
60-64	36.21978924384108	38.0	37.0	38.0	33.6	38.0
65-69	36.134334673728034	38.0	37.0	38.0	33.0	38.0
70-74	36.00811831639193	38.0	37.0	38.0	32.0	38.0
75-79	36.01525201906841	38.0	37.0	38.0	32.2	38.0
80-84	35.227149434796466	38.0	36.6	38.0	28.4	38.0
85-89	35.42830357566675	38.0	37.0	38.0	29.4	38.0
90-94	35.423602926716796	38.0	37.0	38.0	29.0	38.0
95-99	34.48788626762235	38.0	35.4	38.0	23.8	38.0
100-104	34.87931158412955	38.0	36.0	38.0	25.4	38.0
105-109	34.57651709850884	38.0	35.4	38.0	24.0	38.0
110-114	34.13970597210261	38.0	34.6	38.0	23.6	38.0
115-119	34.09193389797178	38.0	34.0	38.0	23.2	38.0
120-124	33.55040652611735	37.0	33.2	38.0	21.8	38.0
125-129	33.282989645281376	37.0	32.0	38.0	21.8	38.0
130-134	33.120072693244005	37.0	32.0	38.0	21.6	38.0
135-139	32.477538330089715	37.0	30.2	38.0	19.4	38.0
140-144	31.081535102292683	35.8	26.6	38.0	14.2	38.0
145-149	30.94176267554537	35.0	26.8	37.6	14.4	38.0
150-154	29.913320765720254	35.0	24.0	37.0	10.2	38.0
155-159	29.642555384521494	35.0	24.0	37.0	10.0	37.4
160-164	28.988843756993	33.6	23.2	37.0	10.2	37.4
165-169	27.65236558191891	31.4	20.6	36.6	9.6	37.0
170-174	27.13188263296015	30.2	20.2	36.0	9.2	37.0
175-179	26.498210471353296	29.0	19.8	35.4	9.0	37.0
180-184	25.33859523018397	27.0	17.4	35.0	8.6	36.8
185-189	24.82907865381877	26.0	16.6	35.0	8.4	36.4
190-194	23.900530280940405	25.0	15.4	34.6	8.0	36.0
195-199	23.055301792241085	24.0	14.8	33.6	8.0	36.0
200-204	22.233699964060243	23.0	12.6	31.4	8.0	35.8
205-209	21.306178925740323	22.0	9.4	30.0	8.0	35.0
210-214	20.17330218554408	20.2	9.0	28.2	8.0	34.8
215-219	19.77074435983156	19.8	9.0	27.8	8.0	34.0
220-224	19.057176762573828	18.6	9.0	26.2	8.0	33.6
225-229	18.192247184153956	16.8	9.0	25.4	8.0	32.4
230-234	17.509047325200548	16.0	9.0	24.8	8.0	31.2
235-239	16.696077141636547	14.8	9.0	23.2	8.0	30.0
240-244	15.787596037133227	13.6	8.0	21.6	7.0	28.8
245-249	15.129763543524401	13.2	8.0	20.6	7.0	27.4
250-254	14.531063477747868	12.4	8.0	19.6	7.0	26.4
255-259	13.827051787155266	11.4	8.0	18.6	7.0	24.8
260-264	13.44113135642067	10.8	8.0	17.8	7.0	24.0
265-269	12.850211908943574	9.8	7.8	16.4	7.0	22.8
270-274	12.315225572832256	9.0	7.0	15.0	7.0	22.0
275-279	11.983359892587595	9.0	7.2	14.2	7.0	21.2
280-284	11.800871369575978	8.4	8.0	14.0	7.0	21.0
285-289	11.499418182804522	8.0	7.6	13.4	7.0	19.8
290-294	11.080433175786098	8.0	7.0	13.0	7.0	19.0
295-299	10.853920484983286	8.0	7.0	13.0	7.0	19.0
300-301	10.570180851568804	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.22261244450262296
1101	2	0.13454530430426814
1101	3	0.2891822609880421
1101	4	0.17119626120478415
1101	5	0.1501488963030937
1101	6	0.1595635584973465
1101	7	1.0798467301806127
1101	8	0.39959297324254806
1101	9	0.2665329692905942
1101	10-14	0.18857472850808676
1101	15-19	0.3149217399797166
1101	20-24	0.2768016203834094
1101	25-29	0.27286276645313023
1101	30-34	0.6344305821734153
1101	35-39	0.2739443223723015
1101	40-44	0.6107936120387762
1101	45-49	0.38275971322632074
1101	50-54	0.32798804216278654
1101	55-59	0.5301113066744563
1101	60-64	0.41891515654132405
1101	65-69	0.4257101153962424
1101	70-74	0.38679855972151245
1101	75-79	0.3867495445872109
1101	80-84	0.8249356851246787
1101	85-89	0.48776841239663327
1101	90-94	0.5047767205821856
1101	95-99	0.9549865551718
1101	100-104	0.4910248202619485
1101	105-109	0.5202308101339312
1101	110-114	0.5236784128681506
1101	115-119	0.42272716604406924
1101	120-124	0.9622013877851501
1101	125-129	0.7035767592694526
1101	130-134	0.8038257609342097
1101	135-139	0.6951405775437394
1101	140-144	1.2266157857039879
1101	145-149	1.654292972257963
1101	150-154	1.1850841271003318
1101	155-159	0.9339319432304052
1101	160-164	1.2262574357943983
1101	165-169	0.7531591314955044
1101	170-174	0.7237049038291481
1101	175-179	1.0723445534705576
1101	180-184	0.9198351597927257
1101	185-189	1.0303390722214942
1101	190-194	1.0119481715247076
1101	195-199	1.0999335151177512
1101	200-204	0.7063451575388875
1101	205-209	0.6981074739552113
1101	210-214	1.0662041440344794
1101	215-219	0.885634577125991
1101	220-224	0.9878734337189066
1101	225-229	0.8257608954219542
1101	230-234	0.7840178454196334
1101	235-239	0.4506809554269715
1101	240-244	0.521530667578217
1101	245-249	0.64179321261072
1101	250-254	0.5217058420005234
1101	255-259	0.27443509363535235
1101	260-264	0.32967281285325534
1101	265-269	0.056709121941853624
1101	270-274	0.24491398144372312
1101	275-279	0.19615793883683708
1101	280-284	0.2850602799556441
1101	285-289	0.04242280882976779
1101	290-294	-0.012779078831428592
1101	295-299	0.033889749549313564
1101	300-301	-0.024442592001593155
1102	1	-0.0936062376503628
1102	2	0.11733630554299879
1102	3	0.1956815883452876
1102	4	0.169571262869745
1102	5	0.09122938699931638
1102	6	-0.016841998464173003
1102	7	1.1951217145300177
1102	8	0.3221491489022057
1102	9	0.3224422357770038
1102	10-14	0.13928733023696793
1102	15-19	0.21471928117076544
1102	20-24	0.20308596845273286
1102	25-29	0.34500869663147427
1102	30-34	0.5530794360168088
1102	35-39	0.23068806546272214
1102	40-44	0.616288637146333
1102	45-49	0.3092971348068261
1102	50-54	0.27819383088636584
1102	55-59	0.437604946537526
1102	60-64	0.41119708043279246
1102	65-69	0.39971480391602654
1102	70-74	0.18457217880591514
1102	75-79	0.4639709306308788
1102	80-84	0.8808889064840884
1102	85-89	0.5604644444703695
1102	90-94	0.6314480047304514
1102	95-99	1.1314866817085587
1102	100-104	0.8778749874236667
1102	105-109	1.024902680347175
1102	110-114	0.9155261158930443
1102	115-119	0.8598863492826041
1102	120-124	1.4459540683664187
1102	125-129	1.0822579798829466
1102	130-134	1.3450927268492023
1102	135-139	1.0268273790941436
1102	140-144	1.8128825518241065
1102	145-149	1.8918584050802707
1102	150-154	1.8141349682367647
1102	155-159	1.5264809262744876
1102	160-164	1.627004002652427
1102	165-169	1.377323655917099
1102	170-174	1.2921266574953805
1102	175-179	1.499471369938977
1102	180-184	1.3725490402088312
1102	185-189	1.4417906486030745
1102	190-194	1.561805651178929
1102	195-199	1.5751109995670518
1102	200-204	1.5752317672862155
1102	205-209	1.4582697073955444
1102	210-214	1.6874543271797862
1102	215-219	1.4269787970601904
1102	220-224	1.562269054215193
1102	225-229	1.35050948686807
1102	230-234	1.2388242441835686
1102	235-239	0.7742554189777273
1102	240-244	0.7772294834605784
1102	245-249	0.3900070464080194
1102	250-254	0.6680982125001407
1102	255-259	0.6479303052181269
1102	260-264	0.4331399199566288
1102	265-269	0.28186063305710896
1102	270-274	0.6472868786026407
1102	275-279	0.5445915469991256
1102	280-284	0.43012601247026083
1102	285-289	0.24764145102378343
1102	290-294	0.02277641752564641
1102	295-299	0.26163792808601727
1102	300-301	0.03666800112279667
1103	1	0.05086220056223567
1103	2	0.16760125337016518
1103	3	0.053944301593986665
1103	4	0.10579430254984601
1103	5	0.019902557029823242
1103	6	0.2080663642170606
1103	7	1.1845368691537033
1103	8	0.3276718899851261
1103	9	0.1600955363539427
1103	10-14	0.19428050235832472
1103	15-19	0.2482013686068072
1103	20-24	0.2388081881340085
1103	25-29	0.05906497925992227
1103	30-34	0.5484377853036264
1103	35-39	0.23041919732245475
1103	40-44	0.5044816341603564
1103	45-49	0.18664719916530714
1103	50-54	0.18814293215458378
1103	55-59	0.40481152743645055
1103	60-64	0.32269307191264573
1103	65-69	0.3806545683052178
1103	70-74	0.4174377970066274
1103	75-79	0.2675348219336584
1103	80-84	0.9219221703407641
1103	85-89	0.5650655852814808
1103	90-94	0.5890445914445124
1103	95-99	1.2827983531340479
1103	100-104	0.7218938964005304
1103	105-109	0.8179398775846636
1103	110-114	0.7594084672968435
1103	115-119	0.9353157869136908
1103	120-124	0.9753884325243121
1103	125-129	1.181187407713253
1103	130-134	1.0339074201827145
1103	135-139	1.2687149445143788
1103	140-144	1.8324230257899607
1103	145-149	1.8409243904836288
1103	150-154	2.030899860526162
1103	155-159	0.759380193829319
1103	160-164	0.9422210246519178
1103	165-169	1.7001483721844366
1103	170-174	2.013926865271973
1103	175-179	1.7903082914477757
1103	180-184	1.568967834189845
1103	185-189	1.2351290565639275
1103	190-194	1.3686632588731982
1103	195-199	1.33802614438995
1103	200-204	1.5477686672332887
1103	205-209	1.2704742954686452
1103	210-214	1.8781677588258567
1103	215-219	1.992072982582478
1103	220-224	1.3728878756384013
1103	225-229	0.7448469212844984
1103	230-234	1.5639972279992058
1103	235-239	1.3552222988658649
1103	240-244	0.7376735242579731
1103	245-249	1.0395829992074486
1103	250-254	0.8361607284264156
1103	255-259	0.9693332546003504
1103	260-264	0.6485990212901864
1103	265-269	0.3571600469703
1103	270-274	0.4560366907543063
1103	275-279	0.46453515982900306
1103	280-284	0.5775232512630666
1103	285-289	0.48886419668040304
1103	290-294	0.38504928438528196
1103	295-299	0.1729137286466198
1103	300-301	-0.13155916795377287
2116	1	0.2010828815206196
2116	2	0.20123579798246283
2116	3	0.1669660866005458
2116	4	0.1434596524224574
2116	5	0.2362278008726335
2116	6	0.3052078059135397
2116	7	0.9714381996732939
2116	8	0.5396269431287024
2116	9	0.2765304772912245
2116	10-14	0.39323017680565897
2116	15-19	0.5273659978885377
2116	20-24	0.3078073388661835
2116	25-29	0.34289253928771046
2116	30-34	0.6650467618072256
2116	35-39	0.3481711467781565
2116	40-44	0.716096980172658
2116	45-49	0.42499582360877497
2116	50-54	0.5808466277564008
2116	55-59	0.8472221612990793
2116	60-64	0.701004894750227
2116	65-69	0.6640626414966562
2116	70-74	0.6407765776639067
2116	75-79	0.7589244042482974
2116	80-84	1.304277666133025
2116	85-89	0.8965422175485074
2116	90-94	0.9088708132320917
2116	95-99	1.6337389815922378
2116	100-104	0.9575448087200584
2116	105-109	1.1467528971178282
2116	110-114	1.2181855216242496
2116	115-119	1.2636877662224393
2116	120-124	1.2858530849678758
2116	125-129	1.3778855573388071
2116	130-134	1.4741598200077135
2116	135-139	1.938428573976239
2116	140-144	2.348812286412244
2116	145-149	2.452506602182936
2116	150-154	2.7896412070016936
2116	155-159	2.5216260568655855
2116	160-164	2.106899847000527
2116	165-169	2.5191603906109776
2116	170-174	2.3456161361884114
2116	175-179	2.680581922968482
2116	180-184	2.713081185709523
2116	185-189	2.1019709383355476
2116	190-194	2.3893844288039716
2116	195-199	1.8486107880834943
2116	200-204	1.2880027305938135
2116	205-209	1.146264050210391
2116	210-214	2.3735955453250703
2116	215-219	2.0765558299458853
2116	220-224	1.9319634604751066
2116	225-229	1.4246053946612385
2116	230-234	1.5369380752082051
2116	235-239	0.8514469173387305
2116	240-244	1.218470098877729
2116	245-249	1.497888774228679
2116	250-254	1.1114827424779374
2116	255-259	1.8048709312094573
2116	260-264	0.8351873451443197
2116	265-269	0.863065031915097
2116	270-274	0.6027814857719314
2116	275-279	0.7737207634525465
2116	280-284	0.341959839265229
2116	285-289	0.420702411159132
2116	290-294	0.39710760077614893
2116	295-299	0.38947445129633707
2116	300-301	0.27061177293204075
1117	1	-0.04284294968142888
1117	2	0.02353536998517569
1117	3	-0.031207748150741565
1117	4	0.023075629247969687
1117	5	0.14544801069569502
1117	6	-0.06534258815713656
1117	7	0.7115668598330132
1117	8	0.03325591645716486
1117	9	0.0733002721656959
1117	10-14	0.04940155840176885
1117	15-19	-0.011628730437571733
1117	20-24	0.15200066837189752
1117	25-29	0.10696621223515024
1117	30-34	0.40962502789626853
1117	35-39	0.11358100637416868
1117	40-44	0.33616261512229073
1117	45-49	0.0068118108732448945
1117	50-54	0.07951500815204326
1117	55-59	0.12074635358468555
1117	60-64	0.24752415462809552
1117	65-69	0.18297216182652676
1117	70-74	0.21196374818428865
1117	75-79	0.30481014985089416
1117	80-84	0.8319298139486762
1117	85-89	0.43898636912218336
1117	90-94	0.2829606665647333
1117	95-99	0.958688091671057
1117	100-104	0.3603783334342907
1117	105-109	0.3279829978537734
1117	110-114	0.5742270686778497
1117	115-119	0.5002319491129796
1117	120-124	0.9084442953650864
1117	125-129	0.7763458110054842
1117	130-134	0.8635375276124932
1117	135-139	1.0880116680834462
1117	140-144	1.4017241033129118
1117	145-149	1.724966020732964
1117	150-154	2.1468682282449834
1117	155-159	1.4218707199482061
1117	160-164	1.8944787916218502
1117	165-169	1.3236152160193981
1117	170-174	1.0170368783983363
1117	175-179	1.4036813801426504
1117	180-184	1.1595665189732571
1117	185-189	0.8140214106984054
1117	190-194	-1.0906008261675844
1117	195-199	1.281487634474221
1117	200-204	1.6537616428543735
1117	205-209	1.2817858676330118
1117	210-214	1.7788169817257184
1117	215-219	1.6608112712528431
1117	220-224	1.5578364183539115
1117	225-229	1.3271198787157275
1117	230-234	1.2081350322518887
1117	235-239	0.8158135335504326
1117	240-244	1.0577629899471983
1117	245-249	1.0450147985514509
1117	250-254	0.8070298743741748
1117	255-259	0.7056897369926372
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2108	245-249	-1.2976949120760342
2108	250-254	-0.8752445710889987
2108	255-259	-0.6849676646062282
2108	260-264	-0.524730187906636
2108	265-269	-0.3995777610511464
2108	270-274	-0.4526000441985438
2108	275-279	-0.4312304623207819
2108	280-284	-0.7213218318938015
2108	285-289	-1.1954575811396744
2108	290-294	-0.9846931950138647
2108	295-299	-0.7582626812925479
2108	300-301	2.455428388257415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	65.0
11	326.0
12	566.0
13	604.0
14	842.0
15	1414.0
16	1968.0
17	2985.0
18	4014.0
19	4133.0
20	5170.0
21	6848.0
22	9019.0
23	11630.0
24	15633.0
25	21557.0
26	29262.0
27	37949.0
28	44583.0
29	44223.0
30	31954.0
31	15672.0
32	4033.0
33	458.0
34	23.0
35	3.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.66076260095342	17.77560029565536	12.293431161803497	37.270205941587726
2	54.933240206416265	20.467691514826843	12.771158684198033	11.827909594558857
3	54.51925489424896	19.11656009059531	15.03400714726485	11.330177867890878
4	54.690138266347496	18.05464199255437	15.17980050044416	12.075419240653968
5	32.02537482453939	18.278417836969126	38.12055415036381	11.575653188127674
6	31.033640968610353	18.06854321925286	38.20497867348392	12.692837138652868
7	14.286731448643444	56.47966691304613	16.328855556082974	12.904746082227451
8	29.936461222358595	16.911689914490502	39.77852972489133	13.373319138259564
9	13.299744353050471	15.88503346466037	40.391200862554165	30.424021319734994
10-14	28.674975757616856	36.84421810685636	21.28684672710875	13.19395940841804
15-19	21.718259430795626	38.672127701415214	18.05626945324102	21.55334341454814
20-24	27.931972143297912	30.787284107168283	24.74147108883901	16.539272660694788
25-29	18.232509883433128	25.734628972868872	39.83054065600228	16.20232048769572
30-34	18.01212458211556	30.22648827889251	30.8556374560077	20.905749682984222
35-39	20.057571421790342	31.67872569828235	28.47405217367718	19.78965070625013
40-44	19.46293797340458	32.27763123097058	27.413422482013168	20.846008313611676
45-49	20.703808936115387	30.364958058981887	26.03360706317938	22.897625941723344
50-54	22.9252249625345	31.806481362184595	24.64219598695319	20.626097688327715
55-59	22.22684089537462	27.008795068794118	28.11655330950912	22.64781072632214
60-64	19.804704717601666	27.28559900724898	29.33443639002095	23.5752598851284
65-69	17.219415605991767	28.032874705870388	29.72550163085123	25.022208057286615
70-74	20.445585173833145	24.86529372274851	31.776644582929293	22.912476520489054
75-79	21.47698838399935	24.565298469508846	29.37946280235168	24.578250344140123
80-84	23.873898921129186	27.826458442113257	23.94482908272247	24.354813554035086
85-89	22.424441747079047	27.384535054825083	24.97019712617567	25.2208260719202
90-94	22.173202503577023	30.71404837626891	27.8353416650279	19.27740745512616
95-99	24.025049332402066	27.197309264998065	28.14082959808502	20.63681180451485
100-104	21.595318338091396	27.328930148031112	27.82693311814686	23.248818395730627
105-109	25.34498776013942	28.773437129159348	23.62299873193688	22.25857637876435
110-114	19.824302056703445	27.510188582007068	31.524388176498114	21.14112118479138
115-119	25.0146132407489	25.910462537889316	27.696193776319088	21.378730445042688
120-124	20.219368138388408	27.80733577904509	26.37408540099953	25.599210681566976
125-129	19.3201282981508	24.814706819738387	30.495833022533546	25.369331859577265
130-134	23.664973655480136	27.782856057883354	27.256643769198952	21.295526517437562
135-139	22.421322447429628	25.618875831530698	25.070964067024253	26.88883765401542
140-144	21.145054214784125	27.13329581132306	26.336246940034858	25.385403033857962
145-149	23.140404519190145	23.454708077431917	29.97940582726256	23.42548157611537
150-154	21.273081122134144	27.42162759630838	24.98911635665801	26.31617492489947
155-159	21.66835063640494	25.36451728837925	31.756097891760305	21.211034183455507
160-164	22.116663242215818	30.240410011873692	24.896300169052594	22.7466265768579
165-169	20.73391695881846	28.253395628911836	25.590124026066498	25.4225633862032
170-174	20.374329773938776	30.802765871312527	26.584723965527644	22.23818038922105
175-179	19.565671429249537	28.55969729231228	26.84401467427052	25.030616604167655
180-184	20.788504702683277	28.069492571320076	27.202056025334137	23.939946700662514
185-189	20.90764838711865	26.129966298001612	29.294427981474076	23.667957333405663
190-194	20.010931118357835	25.837244327448023	28.27728422738625	25.87454032680789
195-199	18.240782808590282	31.593012768785307	27.19697021068835	22.96923421193607
200-204	21.118215433898264	26.644465375411357	25.281398315170183	26.955920875520196
205-209	21.632357737800987	24.42399719534085	29.096643427868518	24.847001638989642
210-214	22.865890458333617	25.82325776942951	29.010571713377047	22.30028005885983
215-219	21.1397649675525	27.094168943981444	29.419742454346338	22.346323634119713
220-224	20.301568669733076	26.032607553185787	27.951656247520663	25.714167529560473
225-229	23.62810056900133	26.519083006654288	27.502883658859595	22.34993276548479
230-234	22.17788145305115	25.890187090168105	28.371250906970282	23.56068054981047
235-239	21.668960934162435	26.354894927069417	26.241989841932885	25.734154296835264
240-244	22.300890356617323	29.753439705972102	24.87071859170402	23.074951345706555
245-249	22.625094087570947	27.18923977242675	25.129959516915417	25.055706623086888
250-254	20.268734445883542	27.336931829740486	23.944354406688863	28.449979317687106
255-259	22.396790102862365	27.666511379687513	24.91752498323375	25.019173534216364
260-264	20.620672819372206	26.580094799584998	27.754850171900536	25.044382209142263
265-269	21.003329513321447	27.57257457499542	25.119923509347725	26.304172402335407
270-274	19.387939160094664	29.734520475489763	25.065878252378464	25.811662112037105
275-279	20.201601692559116	27.607904034068177	25.766161023672773	26.424333249699938
280-284	18.000325492137332	28.74956770575511	28.862269358305813	24.38783744380175
285-289	19.863293302321168	28.754450087815066	25.79518407258475	25.58707253727902
290-294	20.731882632960147	28.671856457967436	25.739375733204945	24.856885175867472
295-299	19.59883094074009	29.24072177881453	24.590320677566133	26.570126602879252
300-301	15.139113983277841	30.025462978659924	29.740826885650545	25.09459615241169
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.75
25	0.25
26	0.0
27	0.25
28	1.75
29	1.25
30	1.5
31	6.25
32	17.0
33	60.5
34	133.75
35	254.75
36	571.25
37	1259.0
38	2532.0
39	4307.0
40	5600.5
41	6087.75
42	6968.5
43	8846.0
44	10119.5
45	9679.25
46	8601.75
47	7794.0
48	6693.75
49	5313.25
50	4250.75
51	3025.25
52	1658.75
53	744.25
54	346.25
55	201.0
56	168.25
57	176.75
58	207.75
59	280.0
60	378.25
61	481.25
62	493.25
63	418.25
64	311.25
65	191.25
66	87.75
67	39.75
68	21.25
69	14.0
70	10.0
71	7.25
72	6.5
73	2.75
74	2.75
75	3.0
76	2.0
77	0.75
78	1.75
79	1.0
80	1.0
81	1.0
82	1.25
83	1.0
84	0.25
85	1.25
86	0.5
87	0.75
88	0.5
89	1.5
90	1.25
91	0.5
92	0.5
93	0.5
94	0.25
95	0.25
96	0.0
97	0.25
98	0.0
99	0.5
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	6.781086194386617E-5
150-154	0.0
155-159	0.0
160-164	6.781086194386617E-5
165-169	0.0
170-174	6.781086194386617E-5
175-179	0.0
180-184	0.0
185-189	0.0
190-194	6.781086194386617E-5
195-199	0.0
200-204	6.781086194386617E-5
205-209	6.781086194386617E-5
210-214	0.0
215-219	0.0
220-224	6.781086194386617E-5
225-229	6.781086194386617E-5
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	6.781086194386617E-5
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	294938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.38510557871442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.02009288542309	8.851249252996169
2	10.392804270043591	3.4057439044715663
3	7.146281617675164	3.512777364025009
4	5.000162601821236	3.2771276856632134
5	3.957532415610027	3.2422293230477504
6	3.2923490726747358	3.2367292294654835
7	2.4172222199822335	2.772450889713635
8	1.8515716166773122	2.427055714064697
9	1.541446158491293	2.273108224570524
>10	10.036884054857287	28.615872497121682
>50	0.2253555630760662	2.370002629054753
>100	0.05164091605097047	1.6068386356593591
>500	0.021502131489378108	2.3115367114074625
>1k	0.030102984085129226	11.995963037145621
>5k	0.010751065744689009	11.067966131514279
>10k+	0.004300426297875604	9.03334877007882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	15595	5.287551960072964	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	10045	3.405800541130678	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6654	2.2560673768724273	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	6601	2.2380974984573028	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	6376	2.1618102787704534	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	5947	2.016355979900861	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	5837	1.9790600058317342	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	4427	1.5009934291274776	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	4148	1.4063972767157844	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3632	1.2314452529006097	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	2848	0.9656266740806543	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2843	0.9639314025320576	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	2713	0.9198543422685446	No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT	2701	0.9157856905519126	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	1928	0.6536967091388699	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1876	0.6360658850334646	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1655	0.5611348825854926	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	1591	0.5394354067634554	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	1409	0.4777275223945372	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1234	0.41839301819365426	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1044	0.35397269934698145	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	884	0.29972400979188846	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	794	0.2692091219171487	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	739	0.25056113488258547	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	685	0.23225220215774162	No Hit
GGGGGGAGCGAAAGCAGGTGAAGCATCGACAACCATCTCAATTTCATTAT	646	0.21902908407868774	No Hit
GGGGGGAGCGAAAGCAGGCCTTTACTACAAACATGGGGTTGACAAGGTTG	597	0.20241542290244052	No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT	596	0.20207636859272118	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	553	0.18749703327478998	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	547	0.18546270741647397	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	520	0.17630824105405205	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	461	0.1563040367806115	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	456	0.15460876523201486	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	433	0.14681051610847026	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	392	0.13290928940997768	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	3.3905430971933084E-4	0.0	0.0
9	6.781086194386617E-4	0.0	0.0
10-14	0.0021021367202598514	0.0	0.0
15-19	0.002441191029979182	0.0	0.0
20-24	0.002983677925530112	0.0	0.0
25-29	0.0030514887874739777	0.0	0.0
30-34	0.004272084302463569	0.0	0.0
35-39	0.004543327750239034	0.0	0.0
40-44	0.0057639232652286244	0.0	0.0
45-49	0.0071879513660498134	0.0	0.0
50-54	0.011934711702120446	0.0	0.0
55-59	0.013969037560436432	0.0	0.0
60-64	0.018241121862899997	0.0	0.0
65-69	0.022987882198970633	0.0	0.0
70-74	0.03322732235249442	0.0	0.0
75-79	0.04706073818904312	0.0	0.0
80-84	0.06523404918999924	0.0	0.0
85-89	0.08469576656788885	0.0	0.0
90-94	0.11087075927822118	0.0	0.0
95-99	0.1429452969776699	0.0	0.0
100-104	0.180376892770684	0.0	0.0
105-109	0.21896127321674386	0.0	0.0
110-114	0.2652760919244045	0.0	0.0
115-119	0.31755826648312524	0.0	0.0
120-124	0.3766215272362327	0.0	0.0
125-129	0.4560958574344438	0.0	0.0
130-134	0.5463521146817298	0.0	0.0
135-139	0.6370830479626226	0.0	0.0
140-144	0.7365615824342743	0.0	0.0
145-149	0.8661481396090025	0.0	0.0
150-154	1.0233337175948845	0.0	0.0
155-159	1.1991672826153292	0.0	0.0
160-164	1.4081603591263248	0.0	0.0
165-169	1.6610948741769456	0.0	0.0
170-174	1.913825956641735	0.0	0.0
175-179	2.2102272341983737	0.0	0.0
180-184	2.5332103696370085	0.0	0.0
185-189	2.9127477639368275	0.0	0.0
190-194	3.309237873722613	0.0	0.0
195-199	3.7194935884830036	0.0	0.0
200-204	4.166299357831138	0.0	1.3562172388773233E-4
205-209	4.643280960744292	0.0	3.3905430971933084E-4
210-214	5.1212797265866055	0.0	3.3905430971933084E-4
215-219	5.568153306796683	0.0	3.3905430971933084E-4
220-224	6.059103947270273	0.0	3.3905430971933084E-4
225-229	6.53893360638507	0.0	3.3905430971933084E-4
230-234	6.996656924506167	0.0	3.3905430971933084E-4
235-239	7.392129871362795	0.0	3.3905430971933084E-4
240-244	7.748747194325587	0.0	3.3905430971933084E-4
245-249	8.057218805308235	0.0	3.3905430971933084E-4
250-254	8.328936929117306	0.0	3.3905430971933084E-4
255-259	8.539082790281347	0.0	3.3905430971933084E-4
260-264	8.72257898270145	0.0	3.3905430971933084E-4
265-269	8.875356854660978	0.0	3.3905430971933084E-4
270-274	8.9995185428802	0.0	3.3905430971933084E-4
275-279	9.0802134685934	0.0	5.424868955509293E-4
280-284	9.139480161932338	0.0	6.781086194386617E-4
285-289	9.181048220303927	0.0	6.781086194386617E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6805	0.0	293.91623	1
GGGTTAT	6910	0.0	290.09045	2
ATTAGTA	7035	0.0	283.04904	7
TTATTAG	7050	0.0	282.65604	5
GGGGGGA	4685	0.0	281.14728	1
TATTAGT	7120	0.0	279.87714	6
GGTTATT	7185	0.0	278.7822	3
GGAGCGA	3830	0.0	276.51437	5
TTAGTAG	7285	0.0	276.17023	8
TAGTAGA	7260	0.0	276.10538	9
GTTATTA	7310	0.0	274.01505	4
CGAAAGC	3945	0.0	271.44485	9
GAGCGAA	3905	0.0	268.55954	6
GCGAAAG	4005	0.0	266.2734	8
GGGGGAG	5025	0.0	264.47263	2
GGGAGCG	4245	0.0	249.48174	4
GGGGAGC	5410	0.0	245.10628	3
AGCGAAA	4355	0.0	241.14812	7
GCAAAAG	1630	0.0	161.97855	8
CGGGGGA	140	0.0	158.0357	1
>>END_MODULE