FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6271_flu.3520000003bb75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6271_flu.3520000003bb75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362286
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA4496112.41036087510972No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT195745.40291372009959No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG143793.9689637468740164No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG117133.2330810464660518No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC88402.44006116714419No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG84982.345660610677752No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT76192.1030346190578713No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC46401.280756087731792No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT44371.2247230088935261No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT43681.205677282588894No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG42161.1637214797149213No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGTATGCT33890.935448789078242No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA31110.8587138338218977No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTGGCCAAATA25720.7099363486306399No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC25340.6994473979121467No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT22580.6232644926936178No Hit
GGGGGGAGCAAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG19720.5443213372860116No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC18660.515062685281794No Hit
GGGGGGAGCAAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT18050.4982251591284234No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT17370.4794554578426989Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGGACCAAAACCAAAACAAGAAACTAGCTG13970.3856069514140762No Hit
GGGGGGAGCGAAAGCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCC12180.3361984730295954No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT11090.3061117459686546No Hit
GGGGGGAGCGAAAGCAGGGTTTGTTGGGGTTTTCGCAGTCTTTGGCCTTG11060.3052836709119315No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG10930.30169534566613115No Hit
GGGGGGAGCGAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA10860.2997631705337772No Hit
GGGGGGAGCGAAAGCAGGGATACCGTCTTTGTTACGTTTAAAATCTACAA10710.29562279525016144No Hit
GGGGGGAGCGAAAGCAGGGGGAGCGAACAGGATTAGATACCCTGGTAGTC9760.26940041845392865No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGCGGTTTGGTTGAATTTG9620.26553606818922065No Hit
GGGGGGAGCGAAAGCAGGAAAGGAACATCCAAGACATTCTCTCGCTTTGC9490.2619477429434204No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGCCGTGGTTTGGTTGAATTTG9090.25090674218711184No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC8560.236277416185003No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA8170.22551244044760216No Hit
CGGGTTATTAGTAGAAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA7480.2064667141429699No Hit
GGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC6900.19045726304632252No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAACATTCT6800.18769701285724538No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTAATATCCGTGGGAAATCTCATC6520.1799683123278294No Hit
CGGGTTATTAGTAGAAACAAGGAATGTGGAACAAATTTACTTCTAACAGA6470.1785881872332908No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT6410.17693203711984454No Hit
CGGGTTATTAGTAGAAACAAGGGCACCACGGTGACGTCCACCAATTTCAC5970.16478693628790514No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCTGTAAACG5950.16423488625008972No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA5860.16175066107992028No Hit
CGGGTTATTAGTAGAAACAAGGCCGTCTGAAAAAAACCTAAAAACCGAGT5810.1603705359853817No Hit
GGGGGGAGCGAAAGCAGGGACGAGGCGAAAGTGACGGTACCTGCAGAAGA5770.15926643590975087No Hit
GGGGGGAGCGAAAGCAGGTCTACATATGGGCCTTTTTTTAATGAACGAGC5210.1438090348509189No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC5190.14325698481310348No Hit
GGGGGGAGCAAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA5080.1402207096051186No Hit
CGGGTTATTAGTAGAAACAAGGCCGCGTGGAGGTTAGACTACGGTCTAGC4960.13690840937822604No Hit
GGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATA4950.13663238435931835No Hit
GGGGGGAGCAAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC4870.13442418420805663No Hit
GGGGGGAGCGAAAGCAGCATAACGCCTGCCGTATCTTTATCCAAGCGATG4840.13359610915133346No Hit
CGGGTTATTAGTAGAAACAAGGTCGATACAGCCCCAGGTTGTGTAGCCCC4630.1277995837542715No Hit
GGGGGGAGCGAAAGCAGGACTTAGTGATCCGGTGGTTCCGCATGGAAGGG4560.12586740862191748No Hit
GGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC4470.12338318345174806No Hit
GGGGGGAGCAAAAGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCAA4470.12338318345174806No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGATA4310.11896678314922465No Hit
GGGGGGAGCGAAAGCAGGAGGTGAAAGTAAGTTGTTCAGCATTCCATTTC4110.11344628277107037No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT3900.10764975737400836No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT3890.10737373235510067No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG3850.10626963227946983No Hit
GGGGGGAGCGAAAGCAGGTTTCATGATGTTATTGGTTATCCAAATCAGAT3690.10185323197694639No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGTGGTTTGGTTGAATTTG3670.10130118193913097No Hit
GGGGGGAGCGAAAGCAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGA3630.10019708186350011No Hit
CGGGTTATTAGTAGAAACAAGGACAATAGCACATGAAAAAAAATGCTTTG3630.10019708186350011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA98600.0284.528381
GGGTTAT99350.0282.528932
TATTAGT99050.0281.746586
TTATTAG99300.0281.18585
GTTATTA100000.0279.512484
GGTTATT100850.0278.180483
TAGTAGA101200.0277.072639
ATTAGTA100750.0276.846137
GGGGGGA139900.0276.44391
TTAGTAG102650.0273.158788
GGGGGAG146500.0266.70822
AGCGAAA111950.0266.14567
GGGGAGC147050.0265.209143
GCGAAAG114150.0263.988168
CGAAAGC114650.0262.836889
GGAGCGA115200.0259.405365
GGGAGCG116050.0258.52224
GAGCGAA116000.0257.616366
GGGAGCA35400.0253.333334
GCAAAAG37500.0240.326668
Produced by FastQC (version 0.11.2)