##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6205_flu.3520000003ba5d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667174
Sequences flagged as poor quality	0
Sequence length	301
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02908986261455	34.0	34.0	34.0	34.0	34.0
2	33.277390905520896	34.0	34.0	34.0	34.0	34.0
3	33.302328927686034	34.0	34.0	34.0	34.0	34.0
4	33.419093070173595	34.0	34.0	34.0	34.0	34.0
5	33.328908200859146	34.0	34.0	34.0	34.0	34.0
6	36.92456540572624	38.0	38.0	38.0	36.0	38.0
7	36.04068353982619	38.0	38.0	38.0	34.0	38.0
8	36.78363065706997	38.0	38.0	38.0	35.0	38.0
9	36.886665247746464	38.0	38.0	38.0	36.0	38.0
10-14	36.92034671614901	38.0	38.0	38.0	36.4	38.0
15-19	36.74822070404423	38.0	38.0	38.0	35.6	38.0
20-24	36.830699637575805	38.0	38.0	38.0	35.8	38.0
25-29	36.797041551379394	38.0	38.0	38.0	36.0	38.0
30-34	36.44474634802915	38.0	37.6	38.0	33.6	38.0
35-39	36.73035280151804	38.0	38.0	38.0	35.6	38.0
40-44	36.21252506842293	38.0	37.2	38.0	33.2	38.0
45-49	36.590504426131716	38.0	37.8	38.0	34.4	38.0
50-54	36.51762628639606	38.0	37.6	38.0	34.4	38.0
55-59	36.199646269189145	38.0	37.0	38.0	33.4	38.0
60-64	36.405100918201256	38.0	37.0	38.0	34.0	38.0
65-69	36.26032549230036	38.0	37.0	38.0	33.4	38.0
70-74	36.091784452032	38.0	37.0	38.0	32.4	38.0
75-79	36.085373530743105	38.0	37.0	38.0	32.2	38.0
80-84	35.34683156118194	38.0	36.6	38.0	29.0	38.0
85-89	35.59042588590083	38.0	37.0	38.0	30.4	38.0
90-94	35.67929235851517	38.0	37.0	38.0	31.0	38.0
95-99	34.76756138578541	38.0	35.8	38.0	25.4	38.0
100-104	35.12728283776046	38.0	36.0	38.0	27.2	38.0
105-109	34.95466490001109	38.0	35.8	38.0	26.8	38.0
110-114	34.54502333724036	38.0	35.2	38.0	24.0	38.0
115-119	34.5055898461271	38.0	35.0	38.0	24.0	38.0
120-124	33.923039866661476	37.4	34.2	38.0	22.8	38.0
125-129	33.49410318747433	37.0	33.2	38.0	23.0	38.0
130-134	33.50312632086982	37.0	34.0	38.0	23.0	38.0
135-139	33.03524327986402	37.0	31.4	38.0	21.4	38.0
140-144	31.65254101628661	36.2	27.6	38.0	16.8	38.0
145-149	31.46599717614895	35.2	28.0	37.6	17.6	38.0
150-154	30.62914981698927	35.0	26.0	37.0	12.6	38.0
155-159	30.29270475168397	35.0	25.2	37.0	11.6	37.4
160-164	29.673653949344548	34.8	24.4	37.0	10.2	37.4
165-169	28.43579875714581	33.0	22.4	36.6	9.8	37.0
170-174	28.01939703885343	33.0	21.8	36.2	9.4	37.0
175-179	27.259300572264504	30.6	20.2	36.2	9.2	37.0
180-184	26.008231735649172	28.2	19.6	35.0	9.0	37.0
185-189	25.565408424189194	27.2	18.2	35.0	9.0	36.8
190-194	24.462185277004203	25.6	15.6	35.0	8.8	36.0
195-199	23.752857575385132	24.6	15.0	34.8	8.0	36.0
200-204	22.739331268904365	23.4	13.4	33.4	8.0	36.0
205-209	22.0833497708244	23.0	10.2	32.4	8.0	36.0
210-214	20.84914759867741	20.8	9.0	29.2	8.0	35.0
215-219	20.388549313972067	20.4	9.0	28.4	8.0	35.0
220-224	19.837118053161543	20.0	9.0	28.0	8.0	35.0
225-229	19.12091538339324	18.6	9.0	26.0	8.0	34.0
230-234	18.575638738919682	17.4	9.0	25.8	8.0	33.6
235-239	17.5086765971096	15.6	9.0	24.8	8.0	31.8
240-244	16.839839082458248	15.0	9.0	23.8	8.0	31.0
245-249	16.199172329856978	14.2	8.6	22.6	7.4	29.6
250-254	15.26952848882001	13.0	8.0	21.0	7.0	28.0
255-259	14.615063836420484	12.2	8.0	19.8	7.0	26.2
260-264	14.232472788208174	11.6	8.0	19.2	7.0	25.6
265-269	13.659718154484437	11.0	8.0	18.0	7.0	24.8
270-274	13.104422534451283	9.4	8.0	16.2	7.0	23.8
275-279	12.62544343754403	9.0	8.0	15.6	7.0	22.6
280-284	12.258748692245202	9.0	8.0	14.8	7.0	21.8
285-289	11.90119009433821	9.0	8.0	14.0	7.0	21.0
290-294	11.787452148914678	9.0	8.0	14.0	7.0	21.0
295-299	11.37174590136906	8.0	7.0	13.4	7.0	19.4
300-301	11.033773048709932	8.0	7.5	13.0	7.0	17.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.10907075036841718
1101	2	-0.025376668857951756
1101	3	0.033800485156262994
1101	4	0.07417960186887029
1101	5	0.07734923449366704
1101	6	0.0926427165200252
1101	7	0.8414238555163749
1101	8	0.26766934596123804
1101	9	0.06083305883023371
1101	10-14	-0.016501002895374484
1101	15-19	0.009421354153722916
1101	20-24	0.040807843472386196
1101	25-29	-0.06463198064081865
1101	30-34	0.3278894756082451
1101	35-39	0.1043872241847481
1101	40-44	0.3366056732627669
1101	45-49	0.09451740726235158
1101	50-54	0.10320792240590748
1101	55-59	0.20634958059462605
1101	60-64	0.08466216912653124
1101	65-69	0.07301791557640769
1101	70-74	0.13527364344545845
1101	75-79	0.14953040059090483
1101	80-84	0.5152933785541052
1101	85-89	0.09196205996343565
1101	90-94	0.29981767522472325
1101	95-99	0.7581796188954257
1101	100-104	0.16550863501731072
1101	105-109	0.31582098470800446
1101	110-114	0.23744235423902182
1101	115-119	0.21077205410953326
1101	120-124	0.4498827934296372
1101	125-129	0.4295107210314555
1101	130-134	0.6619531940368972
1101	135-139	0.4214019172332186
1101	140-144	0.9451410138818375
1101	145-149	1.0657752402424236
1101	150-154	0.7469808103754119
1101	155-159	0.43866814759496364
1101	160-164	0.8893661244077506
1101	165-169	0.6331828376864586
1101	170-174	0.2690839330944428
1101	175-179	0.5211173627469101
1101	180-184	0.4696305951825259
1101	185-189	0.4066544983287592
1101	190-194	0.8540861277686709
1101	195-199	0.8611140770208507
1101	200-204	0.8187549454478749
1101	205-209	0.26139847110758296
1101	210-214	0.9458964357103206
1101	215-219	0.8223575118268904
1101	220-224	0.3972470553859182
1101	225-229	0.42942578758559335
1101	230-234	0.06342519357612986
1101	235-239	-0.003939645452355478
1101	240-244	0.031360323924918276
1101	245-249	0.13508199881773209
1101	250-254	0.43239640987534145
1101	255-259	0.050965329601709186
1101	260-264	-0.004128267146892739
1101	265-269	-0.23162197225648384
1101	270-274	-0.15268236474880226
1101	275-279	0.05328539812262889
1101	280-284	0.027150883933815706
1101	285-289	-0.13016691704149785
1101	290-294	-0.07856815438141318
1101	295-299	0.10610142544343582
1101	300-301	-0.028486108123233933
1102	1	-0.10620052091068288
1102	2	0.023336169118657324
1102	3	0.05957502201852094
1102	4	0.030787476954529325
1102	5	0.05667242388314264
1102	6	0.017448564772934105
1102	7	1.0001275458176835
1102	8	0.21380694707956138
1102	9	0.2510998149524255
1102	10-14	0.045010299993094804
1102	15-19	0.13333179952097396
1102	20-24	0.16458169311798798
1102	25-29	0.09407177486131246
1102	30-34	0.4535263083348795
1102	35-39	0.17650520115972768
1102	40-44	0.48744223240675666
1102	45-49	0.10317359876498955
1102	50-54	0.17467428236791704
1102	55-59	0.30979981248133726
1102	60-64	0.2826807161522993
1102	65-69	0.1579430943733655
1102	70-74	0.17517537276685857
1102	75-79	0.2890724169784846
1102	80-84	0.7549183955878291
1102	85-89	0.3242414220125198
1102	90-94	0.31817600981317185
1102	95-99	0.7907085696903238
1102	100-104	0.6069818345455218
1102	105-109	0.7975470851837869
1102	110-114	0.6004944177472282
1102	115-119	0.5726009502009433
1102	120-124	1.0065104243642935
1102	125-129	0.8378640547168743
1102	130-134	0.9499733254426346
1102	135-139	0.964520720302616
1102	140-144	1.742989517164375
1102	145-149	1.607189759031023
1102	150-154	1.4024932464541529
1102	155-159	1.0366904762683937
1102	160-164	1.3225981356640197
1102	165-169	1.1928131620670719
1102	170-174	1.1725638965558964
1102	175-179	1.3059963141612165
1102	180-184	1.4240911464033132
1102	185-189	1.1911472000747736
1102	190-194	1.2976621842012825
1102	195-199	1.0163176568067733
1102	200-204	1.3885961097259099
1102	205-209	1.0033227299209386
1102	210-214	1.2542220590464694
1102	215-219	1.2006144069768006
1102	220-224	0.9951877526304713
1102	225-229	0.9350705487321633
1102	230-234	0.9004515014557164
1102	235-239	0.9101437972553619
1102	240-244	0.7313705604539393
1102	245-249	0.4705783042131664
1102	250-254	0.5803384437367356
1102	255-259	0.5337531602595273
1102	260-264	0.6672985159162774
1102	265-269	0.6189035137667407
1102	270-274	0.3650887060377741
1102	275-279	0.5142351263330003
1102	280-284	0.22861346876823418
1102	285-289	0.3127651508742879
1102	290-294	0.29903790616537407
1102	295-299	0.026218421798059666
1102	300-301	-0.18685906725600887
1103	1	0.04960932965440179
1103	2	0.11395340536321186
1103	3	0.22142803833204994
1103	4	0.11032443755343735
1103	5	0.16611588276366263
1103	6	0.2398113030403337
1103	7	1.0831325204760063
1103	8	0.44184692389441693
1103	9	0.3706116387921483
1103	10-14	0.21024877718948431
1103	15-19	0.2794585332840498
1103	20-24	0.27811514471899557
1103	25-29	0.16531614202192912
1103	30-34	0.5420508313121815
1103	35-39	0.2673199405122233
1103	40-44	0.644490575781127
1103	45-49	0.2871426696221562
1103	50-54	0.30489206272181946
1103	55-59	0.516710859969912
1103	60-64	0.3557884822008859
1103	65-69	0.4798885780510673
1103	70-74	0.35147929335968797
1103	75-79	0.5128328995730271
1103	80-84	0.9952991940702489
1103	85-89	0.6558378208985403
1103	90-94	0.5931198521853958
1103	95-99	1.2700993545700072
1103	100-104	0.8689997620509544
1103	105-109	0.9327180561301418
1103	110-114	0.9916518601210171
1103	115-119	0.9705328272415912
1103	120-124	1.3080317057144981
1103	125-129	1.257976779810896
1103	130-134	1.2615904894775483
1103	135-139	1.3093646596699458
1103	140-144	1.8621553202428416
1103	145-149	1.951385568139969
1103	150-154	2.3001411639868614
1103	155-159	1.262539183423197
1103	160-164	1.160511121103049
1103	165-169	2.0310750956849475
1103	170-174	2.2381833283636574
1103	175-179	1.9629976236926936
1103	180-184	1.7186203883778006
1103	185-189	1.5301700671095162
1103	190-194	1.6147171240233327
1103	195-199	1.5740478933225965
1103	200-204	1.9095708617248448
1103	205-209	1.585717435588613
1103	210-214	1.87625907331471
1103	215-219	2.0076126915100794
1103	220-224	1.6642115759549583
1103	225-229	1.1494781272308643
1103	230-234	1.5512728372558833
1103	235-239	0.9599555643031756
1103	240-244	0.7834887422543808
1103	245-249	0.7458433818775525
1103	250-254	0.6229060231722112
1103	255-259	0.6122091231164521
1103	260-264	0.38651525606112713
1103	265-269	0.5343719884065212
1103	270-274	0.5171192604711532
1103	275-279	0.3618544527537271
1103	280-284	0.49035699966322355
1103	285-289	0.5270730020131502
1103	290-294	0.316099871789687
1103	295-299	0.1882747960547384
1103	300-301	-0.11533688816440701
2116	1	0.15248298530173798
2116	2	0.151005422899388
2116	3	0.1464977217288279
2116	4	0.1531469433565107
2116	5	0.20304655825683682
2116	6	0.12292745658745474
2116	7	0.9315499858975329
2116	8	0.236109563931322
2116	9	0.2852426963843513
2116	10-14	0.21145455756488474
2116	15-19	0.23632644476600007
2116	20-24	0.18967663383624966
2116	25-29	0.15978801683032628
2116	30-34	0.5226963902415562
2116	35-39	-0.03120703770816391
2116	40-44	0.36959383689668357
2116	45-49	0.1824093990143112
2116	50-54	0.30835422727768247
2116	55-59	0.5323531975569651
2116	60-64	0.3642746567517392
2116	65-69	0.452445887288043
2116	70-74	0.4132486850540431
2116	75-79	0.4779276731657873
2116	80-84	0.8712689433063261
2116	85-89	0.5016062418565568
2116	90-94	0.6041578691610852
2116	95-99	1.2375757065145834
2116	100-104	0.5133348702524216
2116	105-109	0.4119646825484864
2116	110-114	0.6410619548159602
2116	115-119	0.831621483654537
2116	120-124	1.0499430868598125
2116	125-129	0.8325778999456119
2116	130-134	0.896972673576542
2116	135-139	1.208041742491666
2116	140-144	1.8414420408970926
2116	145-149	1.7916936613653291
2116	150-154	2.188722460427069
2116	155-159	1.9549060305941808
2116	160-164	1.4776300757894951
2116	165-169	1.6804287566995768
2116	170-174	1.673386902111531
2116	175-179	1.9606386444938657
2116	180-184	1.7116472656403197
2116	185-189	1.5479297912457568
2116	190-194	1.3556249615315714
2116	195-199	0.7691988983589333
2116	200-204	0.456290597879736
2116	205-209	0.9552786235978381
2116	210-214	1.7760621332528608
2116	215-219	1.921178057794517
2116	220-224	1.2436987998897777
2116	225-229	1.0050897633549702
2116	230-234	1.2679433400003681
2116	235-239	0.8306883450486993
2116	240-244	0.7828850495399458
2116	245-249	0.9813883183992971
2116	250-254	0.9724529647267612
2116	255-259	1.2722198898151884
2116	260-264	0.7872018979393491
2116	265-269	0.6463963107449171
2116	270-274	0.6050439616342711
2116	275-279	0.6544926823668966
2116	280-284	0.2755325973289633
2116	285-289	0.07731223523416197
2116	290-294	0.20666848124518467
2116	295-299	0.17367105647273995
2116	300-301	-0.08997768522112537
1117	1	0.15003528268538702
1117	2	0.04835078767563772
1117	3	0.13250762998838894
1117	4	0.101791850620927
1117	5	0.13998147336494782
1117	6	0.09240815065446384
1117	7	1.0167148637933323
1117	8	0.2809995601570776
1117	9	0.29810273737726334
1117	10-14	0.09245837275587121
1117	15-19	0.1024807582744458
1117	20-24	0.23951117345642814
1117	25-29	0.1662593349678687
1117	30-34	0.499807212350305
1117	35-39	0.16076103137475428
1117	40-44	0.5308497295875725
1117	45-49	0.2544435316257747
1117	50-54	0.18369642473754055
1117	55-59	0.37836480089263347
1117	60-64	0.363882313289956
1117	65-69	0.46849482991289193
1117	70-74	0.4233917158311016
1117	75-79	0.5595761251708495
1117	80-84	0.9277316749627573
1117	85-89	0.5579466359966716
1117	90-94	0.5189747072981348
1117	95-99	1.1218171805832426
1117	100-104	0.4923009436530279
1117	105-109	0.5019621145399
1117	110-114	0.6966061710670459
1117	115-119	0.7663409914095141
1117	120-124	1.0951634007282323
1117	125-129	1.1474259097072235
1117	130-134	0.9581838403825103
1117	135-139	1.0734641894260974
1117	140-144	1.283790311744859
1117	145-149	1.8072825844555354
1117	150-154	2.1866460874074782
1117	155-159	1.8101907795753078
1117	160-164	1.8729915983868644
1117	165-169	1.2935162680199284
1117	170-174	1.1295855320202293
1117	175-179	1.3808620453688896
1117	180-184	1.5438935176651292
1117	185-189	1.380864548908601
1117	190-194	-0.6401734716975049
1117	195-199	1.4169366859544716
1117	200-204	2.0084368848535803
1117	205-209	1.7263762425237736
1117	210-214	2.1934133006492473
1117	215-219	1.7147973341747758
1117	220-224	1.6749763190855624
1117	225-229	1.3418166348507405
1117	230-234	1.1245089824580248
1117	235-239	1.1799517328963987
1117	240-244	0.9782326687039529
1117	245-249	1.222577588884878
1117	250-254	0.9367139036021239
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2108	230-234	-0.8265424995623754
2108	235-239	-0.8773320553926354
2108	240-244	-0.6881093129939622
2108	245-249	-1.0125885912009327
2108	250-254	-0.9355582784092338
2108	255-259	-0.6927511279493466
2108	260-264	-0.5431184487664069
2108	265-269	-0.516363154188646
2108	270-274	-0.7130002541601712
2108	275-279	-0.47957052211763695
2108	280-284	-1.0840008888381014
2108	285-289	-1.3825392765161233
2108	290-294	-1.1527476769472642
2108	295-299	-0.8448537270746339
2108	300-301	2.633564856503437
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	182.0
11	890.0
12	1240.0
13	1181.0
14	1354.0
15	2196.0
16	3317.0
17	5302.0
18	6856.0
19	7345.0
20	9467.0
21	12233.0
22	16807.0
23	23957.0
24	33353.0
25	46240.0
26	62325.0
27	80289.0
28	94098.0
29	99266.0
30	84713.0
31	52025.0
32	19053.0
33	3300.0
34	167.0
35	8.0
36	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.146819270535122	22.311271122675645	16.777032678131942	31.764876928657294
2	28.647399329110545	26.89793067475651	16.610509402344817	27.844160593788125
3	42.55186802843036	26.558888685710176	19.06998773933037	11.81925554652909
4	43.3275277513812	24.884362999757183	19.310854439771333	12.477254809090283
5	42.093067175879156	24.886461402872413	20.367550294226096	12.652921127022335
6	26.485294690740346	24.51969051551769	34.837688519037016	14.157326274704948
7	16.72921906429207	34.642537029320685	34.91877681084695	13.709467095540292
8	25.61505694166739	36.37446902906888	22.780563990803	15.229910038460732
9	14.533240204204601	21.32082485228741	38.27262453273059	25.8733104107774
10-14	22.83230461618708	34.83747867872549	27.333619115852837	14.996597589234591
15-19	22.891839310284873	32.98992466732816	23.1141801089371	21.004055913449864
20-24	21.313990053569235	30.03066666266971	28.75004121863262	19.90530206512844
25-29	17.25726721964585	28.980086154436474	34.30259572465354	19.46005090126414
30-34	17.165357163198806	31.100342639251526	32.039677805190244	19.694622392359413
35-39	19.02643088609568	32.02687754618735	31.067937299714917	17.87875426800205
40-44	18.300863043224105	29.87682373713604	32.859224130436736	18.963089089203116
45-49	19.909558825733615	29.451207630992815	32.25326526513324	18.385968278140336
50-54	18.435490591659747	32.139981474098214	31.202744711274722	18.22178322296732
55-59	20.50532544733458	32.32997688758854	27.453168139046184	19.71152952603069
60-64	18.277001202085213	31.556145773066696	28.981525059429774	21.185327965418317
65-69	17.96901557914427	32.00304568223582	30.099044627038822	19.928894111581087
70-74	19.67924409524352	32.3465242950115	26.700620827550235	21.273610782194748
75-79	19.775021208860057	35.04231279995923	27.055041113712463	18.127624877468246
80-84	20.207651976845618	32.071303737855494	26.272036979858328	21.44900730544056
85-89	20.02479113394707	32.97742418019887	25.633642797830852	21.364141888023212
90-94	18.867761633396984	32.293254833072034	28.84734716880454	19.991636364726443
95-99	19.8539811203674	30.754795600547986	27.7190657909331	21.672157488151516
100-104	19.332527946232915	28.963328906702003	29.860006535026844	21.84413661203824
105-109	19.741117009955424	29.252938513791005	32.208359438467326	18.797585037786245
110-114	18.708792608824684	28.891953223596843	29.92916390626733	22.470090261311142
115-119	17.88289111985779	30.679912586521656	30.268985302185037	21.16821099143552
120-124	21.341748929064984	29.800411886554336	28.04782560471481	20.810013579665874
125-129	21.331886434423403	30.41449456963251	28.969474230110887	19.2841447658332
130-134	21.236319161118388	29.842769652294603	29.376894183526336	19.54401700306067
135-139	19.701637054201754	27.645022138152864	31.796622770071973	20.85671803757341
140-144	19.346677178667036	30.134747457185085	29.719053800058155	20.79952156408973
145-149	19.554658904573618	31.724857383531134	27.983194788765747	20.737288923129498
150-154	19.34652729273023	28.342441402093005	29.5531600452056	22.757871259971164
155-159	19.583167209753377	30.489347606471473	30.284303644926215	19.643181538848935
160-164	20.708181074202532	28.9791568616283	28.26519019026521	22.04747187390396
165-169	20.473699514669335	28.739519225869113	29.532715603425792	21.254065656035756
170-174	19.110337033517492	31.417741099023644	31.439354651110506	18.032567216348358
175-179	20.16625348110088	30.991735289444733	28.969444252923527	19.87256697653086
180-184	22.580616151109005	28.50371867009206	28.712689643181537	20.202975535617394
185-189	19.57270517136459	30.673347582489725	28.50278937728389	21.251157868861796
190-194	20.044186374169257	29.791029026910522	28.772374223216136	21.392410375704088
195-199	19.8538312344306	30.416622949935103	31.227475890847067	18.502069924787236
200-204	21.234790324563008	28.4181038229907	29.016718277390908	21.330387575055383
205-209	21.561062031793803	28.8229157611058	29.6033718340343	20.012650373066094
210-214	21.50815229610267	28.041380509432322	29.029608467955885	21.420858726509127
215-219	20.285982367418395	30.164904507669664	27.98835086499174	21.560762259920203
220-224	20.498820397677367	29.040190415094113	30.324922733799582	20.136066453428942
225-229	19.14220278368162	30.617320219313104	29.665364657495648	20.575112339509634
230-234	17.895301675425003	31.22702623303666	27.575085360041008	23.30258673149733
235-239	19.99559335345802	30.2021361743713	27.289792467931907	22.512478004238776
240-244	18.310245902867916	30.63584612110184	28.863115169356117	22.19079280667412
245-249	20.49900026080153	30.098115334230652	28.668982904010047	20.73390150095777
250-254	21.023511108046776	31.0753716421803	27.14269440955433	20.758422840218593
255-259	19.47824705399191	31.05124000635516	27.010644899231686	22.459868040421238
260-264	18.340342998977775	32.28902804965421	28.00456852335373	21.36606042801428
265-269	19.378932632266846	32.081346095621235	27.239850473789446	21.299870798322477
270-274	18.535734306192985	31.60668731095636	27.905883622563227	21.95169476028742
275-279	18.3319194093295	32.943789775980484	26.026164089128173	22.69812672556185
280-284	19.281866499593807	30.97608719764259	28.40146648400567	21.340579818757927
285-289	18.987280679402975	33.19107159451657	26.632452703492643	21.18919502258781
290-294	17.708094140359186	33.60041008792309	26.324047399928652	22.36744837178907
295-299	18.372988156013275	32.7445913659705	26.24898452277817	22.633435955238063
300-301	13.641493823200545	34.53162143608714	36.78800432870585	15.038880412006463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.25
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.25
15	0.75
16	0.75
17	1.25
18	0.75
19	0.75
20	2.25
21	1.75
22	1.0
23	2.75
24	4.25
25	4.5
26	6.5
27	8.25
28	13.5
29	36.75
30	123.0
31	518.5
32	1707.0
33	4151.5
34	7173.75
35	10114.75
36	13035.5
37	14849.75
38	16059.25
39	18082.0
40	20749.25
41	21568.5
42	20359.0
43	18307.5
44	16060.0
45	13298.25
46	10043.75
47	6614.25
48	4043.75
49	2321.25
50	1217.0
51	648.25
52	363.75
53	219.25
54	145.0
55	115.0
56	90.5
57	80.0
58	66.5
59	52.75
60	45.25
61	40.75
62	29.5
63	20.0
64	18.5
65	18.75
66	15.25
67	12.0
68	9.5
69	6.5
70	6.25
71	5.0
72	4.75
73	3.75
74	2.75
75	2.75
76	1.75
77	2.0
78	1.75
79	2.0
80	2.5
81	2.5
82	2.25
83	0.75
84	1.25
85	1.0
86	0.75
87	1.5
88	1.0
89	1.0
90	0.5
91	0.75
92	1.5
93	0.75
94	0.5
95	1.0
96	1.0
97	0.75
98	0.75
99	0.25
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	667174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.34519580169821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.68179383907564	7.6147382234337115
2	3.536934264376696	0.8732829206293149
3	2.2248749841367657	0.8239955194040572
4	1.853103907597711	0.9150772232074325
5	1.863993535240515	1.1505682582821908
6	1.877153339519012	1.3904295315704331
7	1.6836736044902119	1.4549696219507862
8	1.4958897052816764	1.477364104755754
9	1.5070413187365528	1.6744248144947005
>10	21.484537134236753	54.20333526129909
>50	0.6418878273158972	4.980098165809357
>100	0.09408753432941129	2.2597881839635017
>500	0.006253332771920681	0.6109486823272106
>1k	0.04002106493735859	12.227716910841192
>5k	0.0075039496747898215	6.359888821093785
>10k+	0.0012506582791316368	1.9833737569375307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	12846	1.9254347441596944	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	9223	1.3823979951257095	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	9122	1.367259515508698	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	6507	0.9753077907712231	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA	5786	0.8672400303369135	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTCTATAT	5384	0.8069858837424719	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	5170	0.7749102932668239	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	4376	0.6559008594459615	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	4321	0.6476571329218465	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	4049	0.606888158111677	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	3930	0.5890517316322279	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	3693	0.5535287646101317	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	3483	0.522052717881692	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	3418	0.512310131989556	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	3066	0.45955028223521904	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2980	0.44666009167023896	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2970	0.445161232302218	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	2914	0.43676761984130075	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	2875	0.4309220683060191	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	2722	0.40798951997529875	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	2702	0.40499180123925693	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	2386	0.35762784520979535	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT	2361	0.353880696789743	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2347	0.3517822936745137	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	2298	0.3444378827712111	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA	2282	0.3420397077823776	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2136	0.320156361009272	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1903	0.28523293773438413	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1900	0.28478327992397784	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1774	0.26589765188691405	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	1612	0.2416161301249749	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1590	0.23831863951532883	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1571	0.23547080671608905	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTCTATAT	1471	0.2204822130358797	No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	1417	0.21238837244856665	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	1346	0.20174647093561798	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	1229	0.18420981632977304	No Hit
GGGGGGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1044	0.15648091802138572	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	1031	0.1545324008429585	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	992	0.14868684930767687	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	939	0.1407428946571659	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	860	0.1289019056498005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.4965781040628083E-4	0.0	0.0
2	5.995437472083744E-4	0.0	0.0
3	5.995437472083744E-4	0.0	0.0
4	5.995437472083744E-4	0.0	0.0
5	8.993156208125617E-4	0.0	0.0
6	0.0010492015576146552	0.0	0.0
7	0.0011990874944167488	0.0	0.0
8	0.0017986312416251233	0.0	0.0
9	0.0019485171784272169	0.0	0.0
10-14	0.003027695923402291	0.0	0.0
15-19	0.004826327165027414	0.0	0.0
20-24	0.005695665598479556	0.0	0.0
25-29	0.006475072469850444	0.0	0.0
30-34	0.013849460560513449	0.0	0.0
35-39	0.01615770398726569	0.0	0.0
40-44	0.024491362073462093	0.0	0.0
45-49	0.038130982322452614	0.0	0.0
50-54	0.04688432103169489	0.0	0.0
55-59	0.0606738272174875	0.0	0.0
60-64	0.08354642117348698	0.0	0.0
65-69	0.11499249071456621	0.0	0.0
70-74	0.16211662924514442	0.0	0.0
75-79	0.2213215742819714	0.0	0.0
80-84	0.30963436824576496	0.0	0.0
85-89	0.4242371555246458	0.0	0.0
90-94	0.5619823314457698	0.0	0.0
95-99	0.7404665049897028	0.0	0.0
100-104	0.9869988938417864	0.0	0.0
105-109	1.2716622650163227	0.0	0.0
110-114	1.582405789194423	0.0	0.0
115-119	1.92081825730619	0.0	0.0
120-124	2.299130361794674	0.0	0.0
125-129	2.6992358814941833	0.0	0.0
130-134	3.1249419191994887	0.0	0.0
135-139	3.5289444732558524	0.0	0.0
140-144	4.1007892993432	0.0	0.0
145-149	4.848570238048845	0.0	0.0
150-154	5.455818122408847	0.0	0.0
155-159	6.504150341590051	0.0	0.0
160-164	6.992568655253352	0.0	0.0
165-169	7.49921309883179	0.0	0.0
170-174	7.971293845383663	0.0	0.0
175-179	8.447541420978636	0.0	0.0
180-184	8.913506821308983	0.0	0.0
185-189	9.422429531126813	0.0	0.0
190-194	9.92838449939596	0.0	0.0
195-199	10.444741551679172	0.0	0.0
200-204	10.92995230629491	0.0	0.0
205-209	11.425505190549991	0.0	0.0
210-214	11.961617209303721	0.0	1.498859368020936E-4
215-219	12.453422945138751	0.0	1.498859368020936E-4
220-224	12.951463935944748	0.0	1.498859368020936E-4
225-229	13.405918096328694	0.0	1.498859368020936E-4
230-234	13.880576880993564	0.0	1.498859368020936E-4
235-239	14.296510355619375	0.0	1.498859368020936E-4
240-244	14.68135748695243	0.0	1.498859368020936E-4
245-249	15.003971977325255	0.0	1.498859368020936E-4
250-254	15.307191227475892	0.0	1.498859368020936E-4
255-259	15.53678650546934	0.0	2.997718736041872E-4
260-264	15.747376246676279	0.0	2.997718736041872E-4
265-269	15.91204093684706	0.0	4.4965781040628083E-4
270-274	16.04702821153102	0.0	4.4965781040628083E-4
275-279	16.144933705450153	0.0	4.4965781040628083E-4
280-284	16.218857449480943	0.0	4.4965781040628083E-4
285-289	16.276263763276145	0.0	5.995437472083745E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	9265	0.0	288.47275	1
CGGGTTA	9415	0.0	284.50345	2
TTATTAG	10045	0.0	265.0448	6
GGGGGGA	7515	0.0	259.67065	1
GTTATTA	10595	0.0	252.12129	5
TATTAGT	10655	0.0	250.00937	7
GGGTTAT	10805	0.0	248.58632	3
ATTAGTA	10865	0.0	247.89232	8
GGGGAGC	8060	0.0	243.39331	3
GGAGCAG	8130	0.0	236.0363	5
GGGAGCA	8315	0.0	235.39688	4
GAGCAGA	8195	0.0	234.5241	6
CAGAAGC	8400	0.0	232.1369	9
TTAGTAG	11900	0.0	225.34033	9
GGGGGAG	8755	0.0	224.5774	2
GGTTATT	12105	0.0	221.76787	4
CGCGCGG	15	0.0028494294	196.66667	295
GCAGAAG	10055	0.0	193.635	8
AGCAGAA	12515	0.0	152.86258	7
CCGGGTA	155	0.0	152.25807	1
>>END_MODULE