FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_6205_flu.3520000003ba5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_6205_flu.3520000003ba5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667174
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT128461.9254347441596944No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT92231.3823979951257095No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG91221.367259515508698No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA65070.9753077907712231No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA57860.8672400303369135No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTCTATAT53840.8069858837424719No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA51700.7749102932668239No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC43760.6559008594459615No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT43210.6476571329218465No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT40490.606888158111677No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA39300.5890517316322279No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG36930.5535287646101317No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC34830.522052717881692No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT34180.512310131989556No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT30660.45955028223521904No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA29800.44666009167023896No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG29700.445161232302218No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT29140.43676761984130075No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC28750.4309220683060191No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT27220.40798951997529875No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT27020.40499180123925693Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT23860.35762784520979535No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT23610.353880696789743No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA23470.3517822936745137No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT22980.3444378827712111No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA22820.3420397077823776No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC21360.320156361009272No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA19030.28523293773438413No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG19000.28478327992397784No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC17740.26589765188691405No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA16120.2416161301249749No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA15900.23831863951532883No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15710.23547080671608905No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTCTATAT14710.2204822130358797No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA14170.21238837244856665No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA13460.20174647093561798No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA12290.18420981632977304No Hit
GGGGGGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT10440.15648091802138572No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA10310.1545324008429585No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA9920.14868684930767687No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9390.1407428946571659No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG8600.1289019056498005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT92650.0288.472751
CGGGTTA94150.0284.503452
TTATTAG100450.0265.04486
GGGGGGA75150.0259.670651
GTTATTA105950.0252.121295
TATTAGT106550.0250.009377
GGGTTAT108050.0248.586323
ATTAGTA108650.0247.892328
GGGGAGC80600.0243.393313
GGAGCAG81300.0236.03635
GGGAGCA83150.0235.396884
GAGCAGA81950.0234.52416
CAGAAGC84000.0232.13699
TTAGTAG119000.0225.340339
GGGGGAG87550.0224.57742
GGTTATT121050.0221.767874
CGCGCGG150.0028494294196.66667295
GCAGAAG100550.0193.6358
AGCAGAA125150.0152.862587
CCGGGTA1550.0152.258071
Produced by FastQC (version 0.11.2)