##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_619_flu.3520000003ba40.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199758
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.17390542556493	34.0	34.0	34.0	34.0	34.0
2	33.48832086825058	34.0	34.0	34.0	34.0	34.0
3	33.458755093663335	34.0	34.0	34.0	34.0	34.0
4	33.48634848166281	34.0	34.0	34.0	34.0	34.0
5	33.476206209513514	34.0	34.0	34.0	34.0	34.0
6	37.031558185404336	38.0	38.0	38.0	36.0	38.0
7	36.18498883649215	38.0	38.0	38.0	34.0	38.0
8	36.9433915037195	38.0	38.0	38.0	36.0	38.0
9	37.01298571271238	38.0	38.0	38.0	36.0	38.0
10-14	37.019686821053476	38.0	38.0	38.0	36.6	38.0
15-19	36.92501326605192	38.0	38.0	38.0	36.6	38.0
20-24	37.01626468026312	38.0	38.0	38.0	36.6	38.0
25-29	36.93845452998128	38.0	38.0	38.0	36.0	38.0
30-34	36.615661950960664	38.0	38.0	38.0	34.6	38.0
35-39	36.892896404649626	38.0	38.0	38.0	35.8	38.0
40-44	36.39870943842048	38.0	37.4	38.0	33.8	38.0
45-49	36.70175712612261	38.0	38.0	38.0	35.0	38.0
50-54	36.65102473993532	38.0	38.0	38.0	34.8	38.0
55-59	36.30603530271629	38.0	37.4	38.0	33.6	38.0
60-64	36.46391934240431	38.0	37.2	38.0	34.0	38.0
65-69	36.38736170766627	38.0	37.0	38.0	34.0	38.0
70-74	36.28031918621532	38.0	37.0	38.0	34.0	38.0
75-79	36.31406401746112	38.0	37.0	38.0	34.0	38.0
80-84	35.625610989297044	38.0	36.8	38.0	30.0	38.0
85-89	35.81756124911142	38.0	37.0	38.0	31.6	38.0
90-94	35.81383674245838	38.0	37.0	38.0	31.6	38.0
95-99	34.89398472151303	38.0	35.8	38.0	26.4	38.0
100-104	35.31825508865727	38.0	36.0	38.0	28.6	38.0
105-109	35.05957909069975	38.0	35.8	38.0	27.2	38.0
110-114	34.56934490733788	38.0	35.0	38.0	24.0	38.0
115-119	34.57548033120075	38.0	35.0	38.0	24.0	38.0
120-124	34.11773646111795	37.4	34.6	38.0	23.6	38.0
125-129	33.80819691827111	37.0	34.0	38.0	23.0	38.0
130-134	33.60295457503579	37.0	33.6	38.0	23.0	38.0
135-139	33.01816698204828	37.0	31.2	38.0	21.2	38.0
140-144	31.67637741667418	36.0	27.8	38.0	17.2	38.0
145-149	31.56964727320057	35.2	28.0	37.6	18.0	38.0
150-154	30.574585248150264	35.0	25.6	37.0	13.8	38.0
155-159	30.271254217603296	35.0	25.2	37.0	13.4	37.4
160-164	29.631726388930606	34.4	24.4	37.0	10.2	37.4
165-169	28.413622483204684	32.6	22.4	36.6	9.8	37.0
170-174	27.816149540944544	32.2	21.4	36.2	9.4	37.0
175-179	27.16652749827291	30.4	20.0	36.0	9.2	37.0
180-184	26.056675577448715	28.0	19.4	35.0	9.0	37.0
185-189	25.37999379248891	26.8	18.2	35.0	9.0	36.6
190-194	24.3598033620681	25.6	16.0	34.8	8.4	36.0
195-199	23.512534166341272	24.8	15.0	34.0	8.0	36.0
200-204	22.639908289029727	23.4	14.0	32.4	8.0	36.0
205-209	21.775154937474348	22.6	10.4	31.2	8.0	35.4
210-214	20.43020654992541	20.4	9.0	28.2	8.0	35.0
215-219	19.927914776880023	20.0	9.0	28.0	8.0	34.2
220-224	19.18375033790887	18.8	9.0	26.2	8.0	33.8
225-229	18.31717678390853	17.0	9.0	25.4	8.0	32.4
230-234	17.508360115740043	15.8	9.0	24.8	8.0	31.4
235-239	16.56406151443246	14.8	9.0	23.0	8.0	29.8
240-244	15.68356811742208	13.6	8.0	21.6	7.0	28.6
245-249	14.88548543737923	12.6	8.0	20.2	7.0	27.2
250-254	14.253476706815245	11.8	8.0	19.4	7.0	25.8
255-259	13.592021345828453	11.0	8.0	18.0	7.0	24.6
260-264	13.088459035432873	10.2	8.0	16.8	7.0	23.6
265-269	12.596093272860161	9.4	7.2	15.8	7.0	22.4
270-274	12.068872335525986	9.0	7.0	14.6	7.0	21.2
275-279	11.67677690004906	8.2	7.0	14.0	7.0	20.8
280-284	11.489629451636478	8.0	7.0	13.6	7.0	20.4
285-289	11.190945043502639	8.0	7.0	13.0	7.0	19.0
290-294	10.846013676548623	8.0	7.0	13.0	7.0	19.0
295-299	10.647360305970222	8.0	7.0	13.0	7.0	18.4
300-301	10.367171777851201	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.12835005679654898
1101	2	0.10793606288109459
1101	3	0.05862880373566526
1101	4	0.07576507042319491
1101	5	0.115325869181639
1101	6	0.029349146964499084
1101	7	0.9174853405523322
1101	8	0.26220798630047426
1101	9	0.0945579328663726
1101	10-14	0.036031812933181584
1101	15-19	0.17758485135674107
1101	20-24	0.0959117839885053
1101	25-29	0.20665218691233633
1101	30-34	0.4953565054955291
1101	35-39	0.24132746407669714
1101	40-44	0.4000422995848254
1101	45-49	-0.04183474245584051
1101	50-54	0.2068259494767659
1101	55-59	0.4320585700680937
1101	60-64	0.04649443729491054
1101	65-69	0.13405098423097428
1101	70-74	0.029063920832470558
1101	75-79	0.14690735551982925
1101	80-84	0.8112674647604763
1101	85-89	0.1454648504078193
1101	90-94	0.008483747546392806
1101	95-99	0.974051061958285
1101	100-104	0.2125689771596484
1101	105-109	0.24396656170166153
1101	110-114	0.24388868456941282
1101	115-119	0.16942853619434572
1101	120-124	0.5174991129630016
1101	125-129	0.4933425283589514
1101	130-134	0.66392843684919
1101	135-139	0.5269177202700845
1101	140-144	0.984373016497841
1101	145-149	0.8304342285492332
1101	150-154	0.14427058806883508
1101	155-159	0.6152768426046649
1101	160-164	0.5579798252349057
1101	165-169	0.15780706544023104
1101	170-174	-0.0785112673594206
1101	175-179	0.4697674163650447
1101	180-184	0.3656025339965723
1101	185-189	0.6755070734426276
1101	190-194	0.4112974513125387
1101	195-199	0.6258429180254907
1101	200-204	0.37965632576610986
1101	205-209	0.40011545397563
1101	210-214	0.4775826441441673
1101	215-219	0.5439317052503405
1101	220-224	0.2965036077319141
1101	225-229	0.27222133349000543
1101	230-234	-0.01598865681596351
1101	235-239	-0.29987907163734207
1101	240-244	0.2434722433217349
1101	245-249	-0.2564719137352931
1101	250-254	0.07663888707982913
1101	255-259	-0.06368065536709899
1101	260-264	0.2046003754343957
1101	265-269	0.17048287779068616
1101	270-274	-0.017347781616992464
1101	275-279	0.18923905379907247
1101	280-284	0.017725912805909516
1101	285-289	-0.04419541492423562
1101	290-294	0.036923515752308234
1101	295-299	-0.18290494893007114
1101	300-301	-0.22301609929912658
1102	1	-0.10571238148463635
1102	2	0.053503593846308206
1102	3	0.03153953364001438
1102	4	-0.06024909316340654
1102	5	0.007790530750924063
1102	6	-0.1834961257268617
1102	7	0.8811100337216473
1102	8	0.004226861677835814
1102	9	0.11695657293634554
1102	10-14	0.04640198475836854
1102	15-19	0.19078915121849604
1102	20-24	0.01400985695072876
1102	25-29	0.014728534851286668
1102	30-34	0.3836017161154004
1102	35-39	0.15205877914596044
1102	40-44	0.3795897419570835
1102	45-49	0.19254722295340798
1102	50-54	0.1317448333184288
1102	55-59	0.2590829472370686
1102	60-64	0.2650164303541871
1102	65-69	0.16712733216992604
1102	70-74	0.0065488967092335315
1102	75-79	0.36365819489573425
1102	80-84	0.7627726985160876
1102	85-89	0.17026241269091713
1102	90-94	0.21459708445353698
1102	95-99	0.7583321611880294
1102	100-104	0.4137314069765381
1102	105-109	0.6581865769373962
1102	110-114	0.5760163261347415
1102	115-119	0.6667573458323517
1102	120-124	0.8825930666350885
1102	125-129	0.681972159033549
1102	130-134	0.9534221309495834
1102	135-139	0.8907744761880423
1102	140-144	1.7331428723226878
1102	145-149	1.9089969912950515
1102	150-154	1.617522005838211
1102	155-159	1.0159434058188381
1102	160-164	1.500513611878251
1102	165-169	1.2144811618767655
1102	170-174	1.3676685142617764
1102	175-179	1.5304437135182205
1102	180-184	1.5744561863397664
1102	185-189	0.960444155518072
1102	190-194	1.4497895371969207
1102	195-199	1.68316947051823
1102	200-204	1.6478693720658022
1102	205-209	1.5390060670727195
1102	210-214	2.3093188120757766
1102	215-219	1.979081805411166
1102	220-224	1.7310033679102297
1102	225-229	1.7132583506725219
1102	230-234	0.7479647331340971
1102	235-239	1.2313922654390872
1102	240-244	1.115736160033073
1102	245-249	0.6739247795468764
1102	250-254	0.6934948247826167
1102	255-259	0.4430054321537096
1102	260-264	0.47969418803491237
1102	265-269	0.6906286047325381
1102	270-274	0.5789596697838508
1102	275-279	0.4576989753632734
1102	280-284	0.5124970947036065
1102	285-289	0.15278071576447516
1102	290-294	0.15779364525601913
1102	295-299	0.15760587126009185
1102	300-301	-0.07528594101681385
1103	1	0.1339470717219271
1103	2	0.1135330778064727
1103	3	0.10900193806401859
1103	4	0.21009342863214897
1103	5	0.22540049604731394
1103	6	0.3371849678600256
1103	7	1.1730823554777032
1103	8	0.4972826131661421
1103	9	0.2792594254036871
1103	10-14	0.10282285770930599
1103	15-19	0.2671370901627128
1103	20-24	0.2966580526452134
1103	25-29	0.16299547049442964
1103	30-34	0.5531923263910485
1103	35-39	0.31259612079311694
1103	40-44	0.5198184189878035
1103	45-49	0.26674734709639125
1103	50-54	0.01839311365587548
1103	55-59	0.24325259991883996
1103	60-64	0.2942556313247593
1103	65-69	0.2818121782608287
1103	70-74	0.2783176521757582
1103	75-79	0.30548944507206954
1103	80-84	0.7220883602828607
1103	85-89	0.4857633578705034
1103	90-94	0.5360956878448917
1103	95-99	1.1169615097194736
1103	100-104	0.6625689771596441
1103	105-109	0.6973247706568912
1103	110-114	0.6797095800918029
1103	115-119	0.7235330138062892
1103	120-124	1.1339170234107598
1103	125-129	1.016476856717162
1103	130-134	1.1269881383417228
1103	135-139	1.0750520486283008
1103	140-144	1.6687013747067994
1103	145-149	1.8322999001910283
1103	150-154	1.8901661104568959
1103	155-159	1.0611723649927214
1103	160-164	1.050144004339387
1103	165-169	1.805941393798438
1103	170-174	2.1263394789092374
1103	175-179	1.8873047297978829
1103	180-184	1.7167219369816387
1103	185-189	1.4307309540396353
1103	190-194	1.529581033402092
1103	195-199	1.765768291159823
1103	200-204	1.6054025944228272
1103	205-209	1.126234856960707
1103	210-214	1.623105032203867
1103	215-219	1.5864690186831787
1103	220-224	1.7498618166871402
1103	225-229	0.5479676021467199
1103	230-234	1.7537874625870238
1103	235-239	0.7698970477656388
1103	240-244	0.5259349298888942
1103	245-249	0.8312146534288836
1103	250-254	0.6538776930499761
1103	255-259	0.7329611356776784
1103	260-264	0.5393018679717105
1103	265-269	0.5100351165966579
1103	270-274	0.37407012883076973
1103	275-279	0.46349278514235515
1103	280-284	0.44869606205964274
1103	285-289	0.4755807044787481
1103	290-294	0.15819217246872697
1103	295-299	0.15440848390574935
1103	300-301	-0.007531024672260855
2116	1	0.12004584847157673
2116	2	-0.24137066642821026
2116	3	-0.12781302741568368
2116	4	0.10585215897277322
2116	5	0.05547039606736348
2116	6	3.0411301715815853E-4
2116	7	0.7824049841768215
2116	8	0.11494167868308836
2116	9	0.26503591722481445
2116	10-14	0.1516576186455012
2116	15-19	0.22797589040334998
2116	20-24	0.19657210999761077
2116	25-29	0.1300099661543257
2116	30-34	0.4467208450262774
2116	35-39	-0.0940021259768713
2116	40-44	0.34424546800376277
2116	45-49	0.14396753215956437
2116	50-54	0.1469497068893162
2116	55-59	0.2683729196254916
2116	60-64	0.287342261513551
2116	65-69	0.4428694160641342
2116	70-74	0.20310924125531216
2116	75-79	0.4535478574248941
2116	80-84	0.8249208867102311
2116	85-89	0.399199058446321
2116	90-94	0.5057443256539287
2116	95-99	1.2151452551000617
2116	100-104	0.609778705867356
2116	105-109	0.3923723255885605
2116	110-114	0.6008486066480785
2116	115-119	0.5346339391221306
2116	120-124	0.8588812139742572
2116	125-129	0.8504514176934705
2116	130-134	1.0192704885866632
2116	135-139	1.3481964036287266
2116	140-144	1.3947620502160092
2116	145-149	1.7039180570077157
2116	150-154	2.1203742349018526
2116	155-159	2.015191616145785
2116	160-164	1.8250501836157476
2116	165-169	1.4670774123620518
2116	170-174	1.5019210241580367
2116	175-179	1.9600516000531343
2116	180-184	2.1072130992127143
2116	185-189	1.5095009715146404
2116	190-194	1.2571528671317154
2116	195-199	0.7210680877622266
2116	200-204	0.719023071690998
2116	205-209	0.4376523376952264
2116	210-214	2.056750862553084
2116	215-219	1.8353811280022292
2116	220-224	1.3018657127538305
2116	225-229	0.8146476376696796
2116	230-234	1.043833155427997
2116	235-239	0.6651437031642722
2116	240-244	0.7682910475730296
2116	245-249	0.8875636665208155
2116	250-254	0.6404863961754241
2116	255-259	1.3828107360443695
2116	260-264	0.7665607272263006
2116	265-269	0.6754338904815516
2116	270-274	0.5979249430514137
2116	275-279	0.7817341120968351
2116	280-284	0.5742955120266977
2116	285-289	0.07431448280401476
2116	290-294	0.05883316138212358
2116	295-299	0.1785848668661867
2116	300-301	-0.035947603008983364
1117	1	0.08054000836697384
1117	2	0.10111768796252107
1117	3	0.1264054329236899
1117	4	0.0742569563446338
1117	5	0.1215671957556026
1117	6	0.04551712113538997
1117	7	0.9136407117041614
1117	8	0.18054679510275662
1117	9	0.12319439948961275
1117	10-14	0.03721706315268136
1117	15-19	0.007854754214370985
1117	20-24	0.058087150042481994
1117	25-29	0.1400898373697217
1117	30-34	0.3638128480710776
1117	35-39	0.13593082299369286
1117	40-44	0.43707818278740973
1117	45-49	0.05615727091185363
1117	50-54	0.03825419723229828
1117	55-59	0.1917395295968234
1117	60-64	0.2521282700287699
1117	65-69	0.30690988706028577
1117	70-74	0.16509156251023427
1117	75-79	0.3828387044676944
1117	80-84	0.76418598411815
1117	85-89	0.22295500872439789
1117	90-94	0.14480947186596893
1117	95-99	0.7326204068845072
1117	100-104	0.3549745961236539
1117	105-109	0.2463795442273593
1117	110-114	0.595056941954212
1117	115-119	0.5232866177033131
1117	120-124	0.9681936950808776
1117	125-129	0.6029293192621168
1117	130-134	0.8374387316748724
1117	135-139	0.6091410910820656
1117	140-144	0.9663988456065624
1117	145-149	1.2841174254678798
1117	150-154	2.0274774191883793
1117	155-159	1.5053211655818082
1117	160-164	1.9478967019518905
1117	165-169	1.3382964590792454
1117	170-174	1.198762232314884
1117	175-179	1.216603916973952
1117	180-184	1.2075521785631054
1117	185-189	1.2364681598511922
1117	190-194	-0.7426031122831418
1117	195-199	0.8160656657668639
1117	200-204	1.658149769364062
1117	205-209	1.8454551690624328
1117	210-214	2.2531575683844416
1117	215-219	1.6211747594291666
1117	220-224	1.9645039759100023
1117	225-229	1.3566282835593881
1117	230-234	1.4825697843746646
1117	235-239	1.3608915534157582
1117	240-244	1.320471025501913
1117	245-249	1.3377321135665277
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2108	260-264	-0.62208880436493
2108	265-269	-0.5531024080705098
2108	270-274	-0.7280811260679219
2108	275-279	-0.31478204184005065
2108	280-284	-0.7783725057160034
2108	285-289	-1.0660630321453919
2108	290-294	-0.7804588461810411
2108	295-299	-0.7804389270839955
2108	300-301	2.762711686353615
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	13.0
11	132.0
12	278.0
13	332.0
14	383.0
15	776.0
16	1214.0
17	1521.0
18	1627.0
19	2103.0
20	2695.0
21	3663.0
22	5258.0
23	7364.0
24	10419.0
25	14720.0
26	20605.0
27	27856.0
28	32538.0
29	31500.0
30	21987.0
31	10122.0
32	2404.0
33	236.0
34	7.0
35	3.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.056658556853794	21.437439301554882	13.511348731965677	35.994553409625645
2	47.94200983189659	23.07141641386077	15.81163207481052	13.174941679432111
3	47.09548553750037	21.686240350824498	17.859610128255188	13.358663983419937
4	47.5019773926451	20.786151243004035	17.69390963065309	14.017961733697772
5	28.444918351204958	20.978383844451788	37.07335876410457	13.503339040238687
6	27.317053634898226	20.320587911372762	37.71914015959311	14.643218294135904
7	16.016880425314632	50.80998007589182	18.714644720111334	14.458494778682205
8	26.551627469237776	19.33739825188478	38.83499033830935	15.27598394056809
9	14.877001171417415	18.382242513441263	39.76361397290722	26.9771423422341
10-14	27.150752410416608	34.81833017951722	23.129987284614383	14.900930125451797
15-19	21.070495299312167	37.10129256400244	20.502508034722013	21.325704101963375
20-24	27.38123128986073	30.89037735660149	25.196988355910655	16.53140299762713
25-29	18.375534396619912	26.443596752070004	38.59750297860411	16.583365872705976
30-34	18.772114258252483	28.863925349673103	31.480691636880625	20.883268755193786
35-39	21.0527738563662	30.747103995834962	28.529020114338348	19.671102033460485
40-44	19.490383363870283	31.05908148860121	28.43100151182931	21.019533635699194
45-49	20.94474313919843	29.709047948017105	26.946905755964718	22.39930315681975
50-54	22.829023117972746	31.178325774186767	25.365292003324026	20.627359104516465
55-59	21.569198730463864	27.786521691246406	29.02241712472091	21.62186245356882
60-64	20.552668729162285	27.149651077804144	29.255298911683138	23.042381281350437
65-69	18.371930035342764	28.34359575085854	29.493086634828142	23.791387578970554
70-74	20.25060322990819	26.077653961293162	30.715365592366762	22.956377216431882
75-79	21.19915097267694	25.9433915037195	28.593898617326968	24.263558906276593
80-84	22.60775538401466	27.804143013045785	25.559326785410345	24.02877481752921
85-89	21.375264069524125	27.42648604811822	27.26088567166271	23.93736421069494
90-94	21.65910752009932	30.036644339650977	27.925890327296027	20.378357812953674
95-99	23.58513801700057	28.33718799747695	27.55233832937855	20.525335656143934
100-104	22.32811702159613	27.23205078144555	27.962134182360654	22.477698014597664
105-109	24.56752670731585	28.451826710319487	24.36968732165921	22.610959260705453
110-114	21.14058010192333	26.966229137256082	30.129757005977233	21.763433754843362
115-119	24.863985422361058	26.813043782977402	27.7049229567777	20.61804783788384
120-124	20.008610418606516	27.888244776179178	27.349392765245952	24.75375203996836
125-129	19.727470238989177	24.782987414771874	29.86914166141031	25.620400684828642
130-134	23.886202304788796	29.117532213978915	26.613001732095835	20.383263749136454
135-139	23.091440643178245	26.714624695882016	25.195086054125493	24.998848606814246
140-144	21.668418786731948	27.858208432202964	26.86480641576307	23.608566365302018
145-149	22.228095996155346	25.33795893030567	29.30926420969373	23.124680863845253
150-154	21.781956166962026	27.455320938335387	25.379809569579194	25.3829133251234
155-159	21.15679972767048	27.126422971795872	30.77673985522482	20.940037445308825
160-164	23.234613882798186	30.24289390162096	24.871694750648285	21.65079746493257
165-169	20.63717097688203	27.922285966018883	27.504680663602958	23.93586239349613
170-174	21.519038036023588	30.198540233682756	26.594279077683996	21.688142652609656
175-179	19.395468516905456	28.431502117562253	28.488070565384117	23.684958800148177
180-184	20.911402797384834	28.984971815897236	27.32145896534807	22.78216642136986
185-189	21.467275403237917	27.209723765756564	28.33738823977012	22.985612591235395
190-194	20.62906116400845	26.45360886672874	28.00278336787513	24.91454660138768
195-199	19.986483645210704	31.03565314029976	26.794921855445086	22.182941359044445
200-204	21.753421640184623	27.135033390402384	26.435186575756664	24.676358393656326
205-209	21.981097127524304	26.447000871053977	28.693018552448464	22.87888344897326
210-214	23.780574495139117	25.993452077013185	28.329979274922657	21.895994152925038
215-219	23.030466864906536	27.323661630572992	28.759298751489304	20.886572753031167
220-224	21.475685579551257	26.79452137085874	27.447211125461806	24.282581924128195
225-229	24.31432032759639	26.91817098689414	27.063146407152654	21.70436227835681
230-234	22.89770622453168	27.13703581333413	27.709128044934374	22.256129917199814
235-239	23.598954735229626	26.829163287577966	25.55952702770352	24.01235494948888
240-244	24.195276284304008	29.50940638172188	24.81632775658547	21.478989577388642
245-249	24.612380980986995	27.825769180708658	24.284784589353116	23.27706524895123
250-254	23.014547602599144	27.569258803151815	23.804603570320086	25.611590023928954
255-259	23.71699756705614	28.329979274922657	24.295797915477728	23.65722524254348
260-264	22.55969723365272	27.940107530111437	26.127514292293675	23.37268094394217
265-269	22.846243955185773	28.21564092551988	24.70779643368476	24.230318685609586
270-274	21.828812863564913	30.29936222829624	23.663432753631895	24.208392154506953
275-279	22.666326254768272	28.847205118193013	23.786081158201426	24.70038746883729
280-284	20.78925499854824	29.55386017080668	26.231940648184306	23.424944182460777
285-289	22.140890477477747	29.65778592096437	24.26526096576858	23.936062635789305
290-294	22.54517966739755	29.996295517576264	23.79469157680794	23.663833238218242
295-299	21.655202795382415	30.534046195897034	22.84253947276204	24.96821153595851
300-301	17.980005807026505	31.931136675377207	28.74753451676529	21.341323000831007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.25
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.25
25	0.25
26	0.5
27	0.5
28	1.25
29	0.25
30	0.5
31	4.25
32	16.0
33	45.0
34	85.25
35	151.0
36	298.25
37	681.75
38	1440.0
39	2457.0
40	3340.0
41	4057.0
42	5174.0
43	6510.75
44	7041.5
45	6714.25
46	6319.75
47	5698.5
48	4755.25
49	3849.0
50	3187.25
51	2273.5
52	1309.75
53	602.25
54	232.75
55	96.5
56	51.25
57	31.75
58	25.5
59	20.75
60	15.5
61	12.75
62	13.0
63	6.25
64	9.75
65	7.75
66	4.25
67	5.0
68	3.75
69	3.25
70	3.0
71	2.25
72	1.25
73	1.5
74	1.0
75	1.5
76	1.25
77	1.5
78	0.5
79	0.75
80	0.75
81	0.0
82	0.75
83	0.5
84	0.5
85	0.0
86	0.75
87	1.0
88	0.5
89	0.5
90	0.25
91	0.5
92	0.25
93	0.5
94	0.25
95	0.25
96	0.25
97	0.0
98	0.25
99	0.25
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	199758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.492556730768424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.31609959694391	13.08178580176855
2	16.16540964827319	8.24195245069431
3	9.824999984483314	7.513931084527192
4	6.203844125207787	6.326073932428157
5	4.376899357981336	5.578917759410156
6	2.7307760046476623	4.1768677330501
7	2.067752513997246	3.6898608787784677
8	1.566100318524027	3.193912097283861
9	1.0126060732644524	2.3232525991753232
>10	4.613942019664652	18.223865156707554
>50	0.030573691635091885	0.5920834633609698
>100	0.04651207334731435	2.696556998691422
>500	0.018198242194137387	3.5912541110328275
>1k	0.022242296015056808	13.867591409375077
>5k	0.00404405382091942	6.902094523716026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	7299	3.6539212447060945	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	6091	3.049189519318375	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC	4127	2.0659998598303946	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	3765	1.8847805845072538	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	3665	1.8347200112135684	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2992	1.497812352947066	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	2792	1.3976912063596951	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	2121	1.0617847595590666	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	1973	0.9876951110844122	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1671	0.8365121797374824	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1436	0.7188698324973218	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1252	0.6267583776369408	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	1109	0.5551717578269707	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	966	0.4835851380170006	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	944	0.4725718118923898	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	888	0.44453789084792594	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	869	0.4350263819221258	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	733	0.3669440022427137	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	713	0.35693188758397665	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	699	0.34992340732286065	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	637	0.31888585188077573	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	518	0.25931376966129016	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	465	0.23278166581563692	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	381	0.19073078424894122	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	354	0.17721442945964616	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	348	0.17421079506202505	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	328	0.16419868040328797	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	325	0.16269686320447743	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	319	0.1596932288068563	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	313	0.1566895944092352	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	250	0.1251514332342134	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	228	0.11413810710960261	No Hit
GGGGGGAGCAAAATTAAACAGAGAAAAAATAGATGGGGTAAAGCTGGAAT	226	0.11313689564372892	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	211	0.10562780964967611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.006057329368536E-4	0.0	0.0
2	5.006057329368536E-4	0.0	0.0
3	5.006057329368536E-4	0.0	0.0
4	5.006057329368536E-4	0.0	0.0
5	0.0010012114658737071	0.0	0.0
6	0.0010012114658737071	0.0	0.0
7	0.0010012114658737071	0.0	0.0
8	0.0010012114658737071	0.0	0.0
9	0.0010012114658737071	0.0	0.0
10-14	0.001101332612461078	0.0	0.0
15-19	0.0016019383453979314	0.0	0.0
20-24	0.0023027863715095265	0.0	0.0
25-29	0.002503028664684268	0.0	0.0
30-34	0.005206299622543277	0.0	0.0
35-39	0.005807026502067501	0.0	0.0
40-44	0.007609207140640175	0.0	0.0
45-49	0.010212356951911814	0.0	0.0
50-54	0.014417445108581384	0.0	0.0
55-59	0.021526046516284706	0.0	0.0
60-64	0.031237797735259663	0.0	0.0
65-69	0.04575536399042842	0.0	0.0
70-74	0.06447801840226673	0.0	0.0
75-79	0.08750588211736202	0.0	0.0
80-84	0.12334925259564074	0.0	0.0
85-89	0.16700207250773436	0.0	0.0
90-94	0.22937754683166628	0.0	0.0
95-99	0.30346719530632066	0.0	0.0
100-104	0.38746883729312465	0.0	0.0
105-109	0.483685259163588	0.0	0.0
110-114	0.5943191261426326	0.0	0.0
115-119	0.7276804933970104	0.0	0.0
120-124	0.8891759028424394	0.0	0.0
125-129	1.0764024469608224	0.0	0.0
130-134	1.307982659017411	0.0	0.0
135-139	1.5565834659938527	0.0	0.0
140-144	1.830314680763724	0.0	0.0
145-149	2.136084662441554	0.0	0.0
150-154	2.4960201844231515	0.0	0.0
155-159	2.9130247599595513	0.0	0.0
160-164	3.3780874858578884	0.0	0.0
165-169	3.919042040869452	0.0	0.0
170-174	4.510557774907638	0.0	0.0
175-179	5.154336747464432	0.0	0.0
180-184	5.834659938525616	0.0	0.0
185-189	6.55533195166151	0.0	0.0
190-194	7.353197368816268	0.0	0.0
195-199	8.170286046115802	0.0	0.0
200-204	8.976561639583895	0.0	0.0
205-209	9.760710459656185	0.0	0.0
210-214	10.607535117492166	0.0	0.0
215-219	11.418216041410108	0.0	0.0
220-224	12.22078715245447	0.0	0.0
225-229	12.99141961773746	0.0	0.0
230-234	13.714494538391454	0.0	0.0
235-239	14.413940868450828	0.0	0.0
240-244	15.002653210384565	0.0	0.0
245-249	15.501957368415782	0.0	0.0
250-254	15.903443166231138	0.0	0.0
255-259	16.212517145746354	0.0	4.0048458634948285E-4
260-264	16.45370898787533	0.0	5.006057329368536E-4
265-269	16.666566545520077	0.0	5.006057329368536E-4
270-274	16.808638452527557	0.0	5.006057329368536E-4
275-279	16.907357903062707	0.0	5.006057329368536E-4
280-284	16.983149611029347	0.0	5.006057329368536E-4
285-289	17.03180848827081	0.0	5.006057329368536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	3905	0.0	292.73367	1
GGGTTAT	3960	0.0	287.92297	2
ATTAGTA	3990	0.0	284.27945	7
TTATTAG	4055	0.0	281.17758	5
GGTTATT	4070	0.0	279.77887	3
TAGTAGA	4090	0.0	278.05014	9
GGGGGGA	2490	0.0	275.4518	1
TATTAGT	4130	0.0	275.35712	6
GTTATTA	4140	0.0	275.0483	4
TTAGTAG	4160	0.0	274.08054	8
GGAGCGA	2025	0.0	269.50616	5
GAGCGAA	2050	0.0	265.5	6
GCGAAAG	2070	0.0	263.64734	8
CGAAAGC	2075	0.0	263.01205	9
GGGGGAG	2770	0.0	250.27075	2
AGCGAAA	2230	0.0	242.74663	7
GGGGAGC	2860	0.0	242.39511	3
GGGAGCG	2315	0.0	236.3823	4
GCAAAAG	955	0.0	135.91623	8
GGGAGCA	1095	0.0	134.7032	4
>>END_MODULE