FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_619_flu.3520000003ba40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_619_flu.3520000003ba40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199758
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA72993.6539212447060945No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC60913.049189519318375No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC41272.0659998598303946No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG37651.8847805845072538No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA36651.8347200112135684No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG29921.497812352947066No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG27921.3976912063596951No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG21211.0617847595590666No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA19730.9876951110844122No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT16710.8365121797374824No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC14360.7188698324973218No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA12520.6267583776369408No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC11090.5551717578269707No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9660.4835851380170006No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA9440.4725718118923898No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT8880.44453789084792594No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8690.4350263819221258No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG7330.3669440022427137No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG7130.35693188758397665No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG6990.34992340732286065No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA6370.31888585188077573No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA5180.25931376966129016No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT4650.23278166581563692Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3810.19073078424894122No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3540.17721442945964616No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT3480.17421079506202505No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA3280.16419868040328797No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG3250.16269686320447743No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT3190.1596932288068563No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG3130.1566895944092352No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT2500.1251514332342134No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2280.11413810710960261No Hit
GGGGGGAGCAAAATTAAACAGAGAAAAAATAGATGGGGTAAAGCTGGAAT2260.11313689564372892No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2110.10562780964967611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA39050.0292.733671
GGGTTAT39600.0287.922972
ATTAGTA39900.0284.279457
TTATTAG40550.0281.177585
GGTTATT40700.0279.778873
TAGTAGA40900.0278.050149
GGGGGGA24900.0275.45181
TATTAGT41300.0275.357126
GTTATTA41400.0275.04834
TTAGTAG41600.0274.080548
GGAGCGA20250.0269.506165
GAGCGAA20500.0265.56
GCGAAAG20700.0263.647348
CGAAAGC20750.0263.012059
GGGGGAG27700.0250.270752
AGCGAAA22300.0242.746637
GGGGAGC28600.0242.395113
GGGAGCG23150.0236.38234
GCAAAAG9550.0135.916238
GGGAGCA10950.0134.70324
Produced by FastQC (version 0.11.2)