##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_6019_flu.3520000003ba19.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322786
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18877832371912	34.0	34.0	34.0	34.0	34.0
2	33.517612288017446	34.0	34.0	34.0	34.0	34.0
3	33.49452578488534	34.0	34.0	34.0	34.0	34.0
4	33.53387693394385	34.0	34.0	34.0	34.0	34.0
5	33.511818976039855	34.0	34.0	34.0	34.0	34.0
6	37.076589443160486	38.0	38.0	38.0	36.0	38.0
7	36.25619140854932	38.0	38.0	38.0	34.0	38.0
8	36.979360938826346	38.0	38.0	38.0	36.0	38.0
9	37.053041333886846	38.0	38.0	38.0	37.0	38.0
10-14	37.076111727274416	38.0	38.0	38.0	36.8	38.0
15-19	36.979725266895095	38.0	38.0	38.0	36.8	38.0
20-24	37.077919736295875	38.0	38.0	38.0	36.8	38.0
25-29	37.006564101293115	38.0	38.0	38.0	36.6	38.0
30-34	36.664282217940055	38.0	38.0	38.0	34.6	38.0
35-39	36.93895522110624	38.0	38.0	38.0	36.0	38.0
40-44	36.482470739127464	38.0	37.6	38.0	34.4	38.0
45-49	36.79413171574976	38.0	38.0	38.0	35.4	38.0
50-54	36.74549639699368	38.0	38.0	38.0	35.4	38.0
55-59	36.41451611903862	38.0	37.4	38.0	34.2	38.0
60-64	36.587016785114585	38.0	37.2	38.0	34.2	38.0
65-69	36.461115413927494	38.0	37.2	38.0	34.0	38.0
70-74	36.33845953665896	38.0	37.0	38.0	34.0	38.0
75-79	36.37555408227123	38.0	37.0	38.0	34.0	38.0
80-84	35.72915244155571	38.0	37.0	38.0	30.8	38.0
85-89	35.95701486433736	38.0	37.0	38.0	32.0	38.0
90-94	35.91019127223609	38.0	37.0	38.0	32.0	38.0
95-99	34.987463520722706	38.0	36.0	38.0	27.0	38.0
100-104	35.34542947959329	38.0	36.0	38.0	28.6	38.0
105-109	35.19766966349222	38.0	35.8	38.0	28.0	38.0
110-114	34.70071564442076	38.0	35.2	38.0	24.2	38.0
115-119	34.730479636663304	38.0	35.0	38.0	24.8	38.0
120-124	34.2881469456544	37.6	34.6	38.0	24.4	38.0
125-129	33.84576406659521	37.0	34.0	38.0	23.0	38.0
130-134	33.575526819626624	37.0	33.6	38.0	23.0	38.0
135-139	32.89406665716605	37.0	30.8	38.0	21.2	38.0
140-144	31.73279634184878	36.0	27.8	38.0	17.4	38.0
145-149	31.545277676231308	35.0	28.0	37.6	18.0	38.0
150-154	30.500482672730538	35.0	25.4	37.0	14.2	38.0
155-159	30.369033353367247	35.0	25.6	37.0	14.8	37.4
160-164	29.557871159220042	34.4	24.0	37.0	10.2	37.4
165-169	28.473618434504594	32.8	22.6	36.6	9.8	37.0
170-174	27.797955921260524	31.8	21.2	36.2	9.4	37.0
175-179	27.03850228944254	29.8	20.2	35.6	9.2	37.0
180-184	26.146627177139038	28.2	20.0	35.0	9.0	37.0
185-189	25.377235072153066	27.0	18.4	35.0	9.0	36.6
190-194	24.33734610546926	25.8	16.0	34.6	8.4	36.0
195-199	23.44826975147621	24.4	15.0	33.6	8.0	36.0
200-204	22.594685023514028	23.4	14.4	32.0	8.0	36.0
205-209	21.69222395023328	22.6	10.2	30.6	8.0	35.2
210-214	20.3671509916787	20.4	9.0	28.2	8.0	34.8
215-219	19.81623242643733	19.8	9.0	27.8	8.0	34.0
220-224	19.07762604326086	18.6	9.0	26.2	8.0	33.4
225-229	18.29507971225518	17.0	9.0	25.4	8.0	32.4
230-234	17.400865588966063	15.8	9.0	24.6	8.0	31.2
235-239	16.401527947308743	14.6	8.4	22.8	7.2	29.4
240-244	15.571617728154257	13.6	8.0	21.4	7.0	28.2
245-249	14.907678771693941	12.8	8.0	20.4	7.0	26.8
250-254	14.18030769612065	12.0	8.0	19.2	7.0	25.6
255-259	13.616516825388956	11.2	8.0	18.0	7.0	24.6
260-264	13.019095623725935	10.2	8.0	16.6	7.0	23.4
265-269	12.624554968307176	9.4	7.6	15.8	7.0	22.4
270-274	12.143680333099947	9.0	7.4	14.6	7.0	21.4
275-279	11.645094892591377	8.0	7.0	14.0	7.0	20.6
280-284	11.527066849243772	8.0	7.0	13.6	7.0	20.2
285-289	11.243573141338224	8.0	7.0	13.0	7.0	19.0
290-294	10.957205083244006	8.0	7.0	13.0	7.0	19.0
295-299	10.650362779054854	8.0	7.0	13.0	7.0	18.2
300-301	10.388883037058608	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.1630827793070111
1101	2	0.023586038915546226
1101	3	0.09316760847262628
1101	4	0.16706138391768377
1101	5	0.11457844136682382
1101	6	0.14270476597624082
1101	7	1.0004960274718542
1101	8	0.21367285218155985
1101	9	0.14891963568384625
1101	10-14	0.11651311356711602
1101	15-19	0.16982420687124034
1101	20-24	0.157174106464403
1101	25-29	0.030348987043360864
1101	30-34	0.5027989897637752
1101	35-39	0.22014603824596435
1101	40-44	0.5312420376876261
1101	45-49	0.21881945985798978
1101	50-54	0.2840150817052205
1101	55-59	0.2631970987742491
1101	60-64	0.3317348732226719
1101	65-69	0.3398304641274805
1101	70-74	0.24865752284749476
1101	75-79	0.3179612836152472
1101	80-84	0.6413872508468401
1101	85-89	0.4018677085702791
1101	90-94	0.3937236500645156
1101	95-99	0.9090395270788889
1101	100-104	0.4225959228923699
1101	105-109	0.45464084384020964
1101	110-114	0.4296474651394746
1101	115-119	0.40395206783017557
1101	120-124	0.7927694888365764
1101	125-129	0.5431492190846825
1101	130-134	0.6286212009441741
1101	135-139	0.3521548530925003
1101	140-144	0.924551244483979
1101	145-149	1.2052365906797462
1101	150-154	0.8469477682172446
1101	155-159	0.5449042924198721
1101	160-164	0.8815769542699634
1101	165-169	0.5508391256693983
1101	170-174	0.2487810098740617
1101	175-179	0.6920902874760273
1101	180-184	0.6621871047744996
1101	185-189	0.6702934453640559
1101	190-194	0.7445839971494941
1101	195-199	0.7744512079582577
1101	200-204	0.6151182467344718
1101	205-209	0.5344515579153075
1101	210-214	0.7937161474510326
1101	215-219	0.5888604648711855
1101	220-224	0.3719943805867949
1101	225-229	0.484876002294385
1101	230-234	0.24915411022943346
1101	235-239	0.15251809568122
1101	240-244	-0.003432986070601274
1101	245-249	-0.04746075627094193
1101	250-254	0.354202328854754
1101	255-259	-0.09822426916133509
1101	260-264	0.26713965760881386
1101	265-269	0.17821107085157806
1101	270-274	0.13822281492942068
1101	275-279	0.3320855496738826
1101	280-284	0.3446945426081012
1101	285-289	0.14905002748372453
1101	290-294	-0.025413275551855463
1101	295-299	0.057337672862022515
1101	300-301	-0.20063314821104505
1102	1	0.003919065628771534
1102	2	0.0839262231820257
1102	3	0.02053826757964572
1102	4	-0.12476001934241054
1102	5	0.05894413945328836
1102	6	0.03696415647942786
1102	7	0.8814882315824164
1102	8	0.24275860696539553
1102	9	0.11807626218987366
1102	10-14	0.04001984638072997
1102	15-19	0.1575463897202809
1102	20-24	0.09661280242471548
1102	25-29	0.1003815880355674
1102	30-34	0.5034821931656168
1102	35-39	0.1166364705634706
1102	40-44	0.49425833818372666
1102	45-49	0.2400412883484222
1102	50-54	0.20689814336361678
1102	55-59	0.47411134399041543
1102	60-64	0.22277668753876156
1102	65-69	0.27840735994605126
1102	70-74	0.1533378913804455
1102	75-79	0.2848953729136099
1102	80-84	0.8178351601310467
1102	85-89	0.4112341153739578
1102	90-94	0.28245788110633185
1102	95-99	0.9460799239605322
1102	100-104	0.8014959937640853
1102	105-109	0.8286139125857801
1102	110-114	0.7723434254513037
1102	115-119	0.5518471776813456
1102	120-124	0.9613676461717944
1102	125-129	0.6237728902398985
1102	130-134	0.9595241066847819
1102	135-139	0.7939606645737172
1102	140-144	1.6951990120247302
1102	145-149	1.803510155527384
1102	150-154	1.600163926969902
1102	155-159	1.1612274674730223
1102	160-164	1.4253558344967594
1102	165-169	1.487802981091086
1102	170-174	1.6050786711072256
1102	175-179	1.2979527963349895
1102	180-184	1.2948958219963274
1102	185-189	1.3100893348041716
1102	190-194	1.3627186534216449
1102	195-199	1.2855553893898666
1102	200-204	1.474140216968344
1102	205-209	0.9853516287870256
1102	210-214	1.7646644110938432
1102	215-219	1.3289540155444683
1102	220-224	1.1030787179361958
1102	225-229	1.0707215019400245
1102	230-234	0.9745431959842179
1102	235-239	1.092858279947695
1102	240-244	0.8355606354744012
1102	245-249	0.1353797823541445
1102	250-254	0.7469734131921033
1102	255-259	0.5329167655658082
1102	260-264	0.43139196093978605
1102	265-269	0.2952854861598695
1102	270-274	0.28945740032984446
1102	275-279	0.22169859007076376
1102	280-284	0.3643061655705413
1102	285-289	0.21247171423071265
1102	290-294	0.05164129567422471
1102	295-299	0.004796212904546238
1102	300-301	-0.06045596890558791
1103	1	0.2754861801503097
1103	2	0.11811704050536775
1103	3	0.14770828725882978
1103	4	0.1403993254397733
1103	5	0.14790255832265586
1103	6	0.2576145606814322
1103	7	1.080095115050483
1103	8	0.25573271255343855
1103	9	0.18038849584836214
1103	10-14	0.06011413658502818
1103	15-19	0.19880132721409893
1103	20-24	0.16232486335940877
1103	25-29	0.15205715044974255
1103	30-34	0.5860441684462927
1103	35-39	0.33347817345023145
1103	40-44	0.5133746152779963
1103	45-49	0.2867387243910713
1103	50-54	0.25037272323421433
1103	55-59	0.4210432313518382
1103	60-64	0.31630803973958166
1103	65-69	0.37179175534196673
1103	70-74	0.37227181744899696
1103	75-79	0.34088987973053264
1103	80-84	0.7505028933435511
1103	85-89	0.5934673491315507
1103	90-94	0.5736384907363927
1103	95-99	1.2109464877480107
1103	100-104	0.5201583753704213
1103	105-109	0.8270616636369894
1103	110-114	0.8377063577917099
1103	115-119	0.8061169949055937
1103	120-124	1.2608460769336247
1103	125-129	1.3288062315959266
1103	130-134	1.0603104246947836
1103	135-139	1.3510914674562287
1103	140-144	1.683843979674414
1103	145-149	1.8428883498558122
1103	150-154	2.1562915160571556
1103	155-159	1.052850929594129
1103	160-164	1.0364220500050862
1103	165-169	1.9259225569267429
1103	170-174	2.3516407596494098
1103	175-179	2.160077015892419
1103	180-184	2.000827043946405
1103	185-189	1.7785760194982956
1103	190-194	1.8184704974605452
1103	195-199	2.1598203930000786
1103	200-204	1.945071934437518
1103	205-209	1.306246677843422
1103	210-214	2.0943103273086443
1103	215-219	1.9853658910134406
1103	220-224	1.281912124417584
1103	225-229	0.7798670854491476
1103	230-234	1.499126322851268
1103	235-239	1.4865320584931325
1103	240-244	1.2335021075908337
1103	245-249	1.3898155276856485
1103	250-254	0.8257181925445298
1103	255-259	0.7415614500617238
1103	260-264	0.7694939812419097
1103	265-269	0.7230070893764982
1103	270-274	0.4788852881443244
1103	275-279	0.5953182862467727
1103	280-284	0.40197552691722116
1103	285-289	0.22358351749547545
1103	290-294	0.24021802326614328
1103	295-299	0.26427124582117045
1103	300-301	-0.12624108351207397
2116	1	0.03956432419504807
2116	2	-0.07069458835282205
2116	3	0.13708735099128688
2116	4	0.1342270348113388
2116	5	0.13347181943982633
2116	6	0.11953144542567173
2116	7	1.0291915010046964
2116	8	0.21848830106192452
2116	9	0.12566003149292726
2116	10-14	0.13192665872907128
2116	15-19	0.18674593528321282
2116	20-24	0.11951362574126989
2116	25-29	0.1655792821881974
2116	30-34	0.5335030916606414
2116	35-39	-0.005452537389182055
2116	40-44	0.35912726675014284
2116	45-49	0.23045350765638517
2116	50-54	0.22689421612965077
2116	55-59	0.4303024880778139
2116	60-64	0.29127606338907697
2116	65-69	0.4387728732215024
2116	70-74	0.2926178049812975
2116	75-79	0.4255400003172909
2116	80-84	0.7896022751569163
2116	85-89	0.4904144455421289
2116	90-94	0.5031156927954541
2116	95-99	1.20532056522174
2116	100-104	0.48549560104068945
2116	105-109	0.602453485078307
2116	110-114	0.8321190120818827
2116	115-119	0.679832366398962
2116	120-124	1.040497763848549
2116	125-129	1.1712598812773862
2116	130-134	1.1398060259801213
2116	135-139	1.434175259858236
2116	140-144	2.0903522442785416
2116	145-149	2.1218545458944362
2116	150-154	2.4127961555349238
2116	155-159	2.1159907814974623
2116	160-164	1.966744111703374
2116	165-169	1.8076074609429682
2116	170-174	1.6592615960549786
2116	175-179	2.3713805702946225
2116	180-184	2.0089196939684975
2116	185-189	1.589893253622634
2116	190-194	1.8883519900276653
2116	195-199	0.9110549195243713
2116	200-204	1.0019812200961091
2116	205-209	1.4715631103360458
2116	210-214	2.3683969664608284
2116	215-219	2.1514158302070783
2116	220-224	1.481959456256181
2116	225-229	1.0021480696778013
2116	230-234	1.5261473308005513
2116	235-239	1.05916878389597
2116	240-244	0.8157589607896334
2116	245-249	0.9528873913699538
2116	250-254	0.9769497095299577
2116	255-259	1.4541841400696427
2116	260-264	0.8132767527262548
2116	265-269	0.574244488642444
2116	270-274	0.710672037691868
2116	275-279	0.6586274931101634
2116	280-284	0.22981102552257227
2116	285-289	0.2897392009412325
2116	290-294	0.17234006280327563
2116	295-299	0.2856313111555231
2116	300-301	-0.16073929078448757
1117	1	0.09472350116952555
1117	2	0.017477759538223836
1117	3	0.029533499091165538
1117	4	0.10411331944599311
1117	5	0.09401087007601205
1117	6	0.2037737837472875
1117	7	0.9313570276096144
1117	8	0.46445946190741694
1117	9	0.2579594483302188
1117	10-14	0.11864178142771209
1117	15-19	0.21085244767576228
1117	20-24	0.16857126860795546
1117	25-29	0.1419351559371549
1117	30-34	0.45685822657324593
1117	35-39	0.16602728910697095
1117	40-44	0.5525292673335827
1117	45-49	0.2361463643903008
1117	50-54	0.28762329220253946
1117	55-59	0.3078203251139655
1117	60-64	0.3643895088474238
1117	65-69	0.3604349551042034
1117	70-74	0.37978522638792356
1117	75-79	0.5067773739858126
1117	80-84	0.9423587345222799
1117	85-89	0.45081990584262854
1117	90-94	0.49680341311727716
1117	95-99	1.092518794195577
1117	100-104	0.5060884149711669
1117	105-109	0.37866949792479687
1117	110-114	0.47465035809992884
1117	115-119	0.8591855710675347
1117	120-124	0.9624799172702438
1117	125-129	0.8180631190709207
1117	130-134	0.9956822286339388
1117	135-139	1.1643096953572822
1117	140-144	1.440045940723131
1117	145-149	1.8700686612128834
1117	150-154	2.2886411144081826
1117	155-159	1.4437922935219518
1117	160-164	2.0418728627973124
1117	165-169	1.3145206244984387
1117	170-174	1.0557301764259321
1117	175-179	1.6082809473465325
1117	180-184	1.4447753400686167
1117	185-189	0.8972183661520461
1117	190-194	-0.9927583365052826
1117	195-199	1.320817374096844
1117	200-204	1.524676037063717
1117	205-209	1.5564545886357912
1117	210-214	2.4930400212628534
1117	215-219	2.0807860744592794
1117	220-224	1.9447812658327024
1117	225-229	1.3173697342133828
1117	230-234	1.3637974495323704
1117	235-239	0.8543723455779002
1117	240-244	0.9978867549405557
1117	245-249	1.2791065394998213
1117	250-254	0.8275512749905847
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2108	245-249	-0.826944939875732
2108	250-254	-0.6134864583306019
2108	255-259	-0.7726138236101168
2108	260-264	-0.6258984363473044
2108	265-269	-0.21616663812675085
2108	270-274	-0.5209415409731246
2108	275-279	-0.203079883089611
2108	280-284	-0.7393937599898255
2108	285-289	-0.9842772036375571
2108	290-294	-0.7537271805188794
2108	295-299	-0.5179354459965051
2108	300-301	2.5766935444797614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	45.0
12	210.0
13	347.0
14	590.0
15	979.0
16	1395.0
17	2037.0
18	2824.0
19	3331.0
20	4594.0
21	5999.0
22	8408.0
23	11842.0
24	16977.0
25	24442.0
26	34649.0
27	46373.0
28	53955.0
29	50628.0
30	34542.0
31	14710.0
32	3557.0
33	336.0
34	10.0
35	2.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.078088888613507	22.750057313514215	13.920987899103432	34.25086589876884
2	45.95149727683357	23.391349067183832	17.030478397452182	13.626675258530419
3	45.03603006326173	22.575328545847714	18.241807265494785	14.146834125395774
4	45.34335442057586	21.5037207313824	18.577943281307117	14.574981566734616
5	27.96279888223157	21.717794452051827	36.085517959267136	14.233888706449473
6	27.222370239105786	20.940809080939076	36.46502636421654	15.371794315738601
7	16.34240642407044	48.8462944489538	19.590378764878277	15.22092036209749
8	26.566517754797296	20.041761414683414	37.60293197350567	15.788788857013625
9	15.436233293885113	18.85459716344575	38.5723048707193	27.136864671949834
10-14	26.68492437714151	34.411963344135124	23.426604623496683	15.476507655226682
15-19	21.127991920343508	36.18347759816101	21.246522463799543	21.442008017695933
20-24	25.741574913410126	31.05977334828648	26.198100289355796	17.0005514489476
25-29	19.325125625027106	25.428426263840436	38.189698437974386	17.056749673158066
30-34	19.270352493602573	28.279974967935413	31.62943869932401	20.820233839138005
35-39	20.46668690711493	30.028997540165932	27.48365790337871	22.020657649340432
40-44	20.0570037114373	28.81277378820643	29.318619766656546	21.81160273369973
45-49	21.222605689218245	30.131542260197158	26.24172052071651	22.404131529868085
50-54	21.9303811193794	30.032343410185074	27.43377965587107	20.60349581456445
55-59	21.66401268952185	27.011518467343688	29.942004919668143	21.38246392346632
60-64	22.33194748223281	25.632276492784694	28.621315670444197	23.4144603545383
65-69	20.19052870942358	27.564454468285486	28.516974094291577	23.728042727999355
70-74	20.295365970023482	26.693784736636655	29.384297955921262	23.6265513374186
75-79	20.292144021116158	26.029257774500753	30.612665976839143	23.065932227543946
80-84	23.053973840253295	25.598074265922317	27.770039592795225	23.577912301029162
85-89	20.58397823945276	27.89953715464735	26.401516794408682	25.114967811491205
90-94	19.892312553828233	29.814985780052417	29.278407365870883	21.014294300248462
95-99	22.23479333056576	30.16605428983909	26.532997094049925	21.06615528554522
100-104	22.72273270835786	28.11708066644774	26.80965097618856	22.35053564900584
105-109	23.23099514848847	27.63248715867479	23.77519471104695	25.36132298178979
110-114	21.74654414999411	28.22129832148854	28.92194828772004	21.11020924079731
115-119	23.83913800474618	27.718550370833928	26.945964199190797	21.496347425229096
120-124	18.310087798107723	28.831361954979464	27.962922803343393	24.895627443569424
125-129	20.55151090815587	24.52435979255606	31.103703382426744	23.820425916861325
130-134	25.56864300186501	28.822873358819773	25.778565365288458	19.829918274026753
135-139	24.679013340107687	27.55980742659223	24.325776210864163	23.435403022435917
140-144	20.76496502326619	29.103678598204386	26.844410848054128	23.2869455304753
145-149	21.169567453359193	26.668133066489872	29.064581487425105	23.09771799272583
150-154	23.39004789550972	24.991046699670992	25.529607851641643	26.089297553177648
155-159	21.655028408914884	26.45734325528369	30.629581208602602	21.258047127198825
160-164	25.67812730415817	28.798460899791195	25.691572744790665	19.831839051259966
165-169	20.62759847081348	25.09272397191948	28.590459313600963	25.689218243666083
170-174	22.82124999225493	28.584635021345413	25.933094991728268	22.661019994671392
175-179	20.365257477090086	28.62968034549206	28.92709101386058	22.07797116355728
180-184	20.090400451072846	29.561195343044616	26.338812711827654	24.009591494054884
185-189	22.460081911854914	27.29597937952699	27.566623087742343	22.677315620875753
190-194	20.75362624153464	27.04937636700476	26.544397836337385	25.652599555123206
195-199	21.73384223603254	29.44799340739685	27.1384756464035	21.67968871016711
200-204	22.805573971609675	26.89162479165763	27.4492697948486	22.853531441884098
205-209	21.88322913633181	26.925207412960912	28.195089006338563	22.996474444368715
210-214	24.227011084743452	26.174245475330405	27.458006233231924	22.14073720669422
215-219	23.7583414398394	26.473886723711686	27.442949818145767	22.32482201830315
220-224	20.243752826950363	26.237940926805997	28.239700606593843	25.278605639649797
225-229	22.511633094372122	27.62189190361416	26.180193688697774	23.686281313315945
230-234	21.95281084061886	26.126040162832343	28.09985563190473	23.821293364644067
235-239	22.680723451450806	26.211112006096922	25.50333657593576	25.604827966516513
240-244	23.524378380722833	30.106634116721292	23.148835451351673	23.220152051204206
245-249	21.841033997757027	28.42260816764048	24.056123871543374	25.680233963059116
250-254	22.219860836591426	26.193763050442087	25.528802364414815	26.057573748551672
255-259	22.14036544335876	26.92570309740819	26.73467870353733	24.199252755695724
260-264	22.016134528758993	27.8367091509545	25.92547384335132	24.221682476935182
265-269	21.216347673071322	27.38030769612065	26.576493404298823	24.826851226509206
270-274	20.421579622412374	29.101076254856157	24.419026847508878	26.058317275222592
275-279	21.523176345938175	28.150849169418745	23.690370710006007	26.63560377463707
280-284	19.1595670196353	28.691083256398976	26.51149678114912	25.637852942816604
285-289	21.124522129212544	27.540475733148277	26.96721666986796	24.367785467771217
290-294	20.358751618719523	29.57934978592628	24.678827458439955	25.383071136914243
295-299	19.923354792339197	30.378950759946218	22.8026618254819	26.895032622232684
300-301	15.385425638038825	30.881605769766967	30.872931291939555	22.860037300254657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.25
24	0.5
25	0.75
26	1.0
27	0.0
28	0.5
29	1.0
30	0.25
31	2.75
32	2.5
33	8.5
34	28.5
35	87.5
36	302.0
37	947.5
38	2023.25
39	3237.5
40	4369.5
41	5664.25
42	7920.0
43	11174.75
44	13002.5
45	12465.75
46	10740.5
47	8860.0
48	7180.75
49	5839.0
50	4686.25
51	3770.75
52	2613.25
53	1405.75
54	583.25
55	234.0
56	108.5
57	61.25
58	45.25
59	36.25
60	33.25
61	36.5
62	36.5
63	35.0
64	27.75
65	21.25
66	12.25
67	6.0
68	6.25
69	3.25
70	3.25
71	1.5
72	2.5
73	1.5
74	1.25
75	1.5
76	0.5
77	0.75
78	0.75
79	1.5
80	1.0
81	0.5
82	0.5
83	1.0
84	0.75
85	0.5
86	0.75
87	0.5
88	0.75
89	0.25
90	0.0
91	0.0
92	0.25
93	0.75
94	0.25
95	0.5
96	0.5
97	0.5
98	0.75
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	322786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.163472934720783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.15320384941986	6.820165994413507
2	5.36470365200639	1.5196566995598049
3	4.731046480307618	2.010241463302289
4	5.598237758360878	3.17161955890705
5	4.899328407907482	3.4695752651853136
6	4.2960790927910235	3.6508439973699285
7	3.994461310034085	3.9602811257419956
8	3.255199260214396	3.6883941295335765
9	2.8762439057384253	3.66638424413236
>10	16.387831378468785	39.090933933547824
>50	0.34360719844293297	3.0719337878416058
>100	0.05464138931929218	1.5617985862766086
>500	0.011354079247349734	1.0748753894835454
>1k	0.02497897434373013	10.739748355821893
>5k	0.004541631698860024	4.4927990172040015
>10k+	0.004541631698860024	8.010748451678722
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14848	4.599951670766391	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10059	3.116306159498863	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	8110	2.5125005421548643	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5859	1.815134485386603	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4656	1.4424417415873054	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4612	1.4288104192870819	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4436	1.3742851300861871	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3721	1.1527761427075522	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3514	1.088646967340591	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2755	0.8535066576617326	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	2199	0.6812563122316333	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1983	0.6143389118487171	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1891	0.5858370561300676	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1877	0.58149981721636	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1748	0.5415352586543407	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	859	0.2661205876339123	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	748	0.23173247910380254	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	661	0.20477963728290569	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	571	0.17689738712335729	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	503	0.1558307981139207	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	483	0.14963474252290992	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	434	0.13445440632493355	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	411	0.12732894239527115	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	334	0.10347412836987974	Illumina Single End PCR Primer 1 (96% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	2.478422236404305E-4	0.0	0.0
15-19	3.7176333546064575E-4	0.0	0.0
20-24	0.0019207772332133365	0.0	0.0
25-29	0.0023545011245840897	0.0	0.0
30-34	0.002788225015954843	0.0	0.0
35-39	0.003469791130966027	0.0	0.0
40-44	0.0039035150223367806	0.0	0.0
45-49	0.004585081137347965	0.0	0.0
50-54	0.006319976702830977	0.0	0.0
55-59	0.009294083386516144	0.0	0.0
60-64	0.014003085635684323	0.0	0.0
65-69	0.02081874678579616	0.0	0.0
70-74	0.032033607405525646	0.0	0.0
75-79	0.04882491805716481	0.0	0.0
80-84	0.08079656490678035	0.0	0.0
85-89	0.12596581016524883	0.0	0.0
90-94	0.18216403437571643	0.0	0.0
95-99	0.2638900076211484	0.0	0.0
100-104	0.3630268970773206	0.0	0.0
105-109	0.49611817117223184	0.0	0.0
110-114	0.6605614865576575	0.0	0.0
115-119	0.8401231775851492	0.0	0.0
120-124	1.0491161326699423	0.0	0.0
125-129	1.292559156840755	0.0	0.0
130-134	1.5859423890751148	0.0	0.0
135-139	1.893514588612889	0.0	0.0
140-144	2.2244459177287736	0.0	0.0
145-149	2.6256405172467203	0.0	0.0
150-154	3.0966027027194487	0.0	0.0
155-159	3.6254360474122174	0.0	0.0
160-164	4.158854473242334	0.0	0.0
165-169	4.727404534273481	0.0	0.0
170-174	5.370740986288129	0.0	0.0
175-179	6.036321277874505	0.0	0.0
180-184	6.751284132521238	0.0	0.0
185-189	7.497722949570303	0.0	0.0
190-194	8.284931812408221	0.0	0.0
195-199	9.033477288358231	0.0	0.0
200-204	9.743731140755795	0.0	0.0
205-209	10.453303427038348	0.0	0.0
210-214	11.133072685928138	0.0	0.0
215-219	11.827712478236354	0.0	0.0
220-224	12.495895113170956	0.0	0.0
225-229	13.133531194041874	0.0	0.0
230-234	13.702762821188031	0.0	0.0
235-239	14.204581363503994	0.0	0.0
240-244	14.629320974267781	0.0	0.0
245-249	14.968369136207892	0.0	0.0
250-254	15.276189177969304	0.0	0.0
255-259	15.520189847143309	0.0	0.0
260-264	15.720570284956597	0.0	0.0
265-269	15.880614400872407	0.0	0.0
270-274	15.99325869151698	0.0	0.0
275-279	16.07510858587423	0.0	0.0
280-284	16.13273190287063	0.0	0.0
285-289	16.1785207536882	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5335	0.0	291.95874	1
GGGTTAT	5365	0.0	290.05124	2
GTTATTA	5405	0.0	287.35892	4
GGTTATT	5450	0.0	284.7156	3
TTAGTAG	5565	0.0	279.62714	8
TTATTAG	5550	0.0	279.05405	5
GGGGGGA	3655	0.0	276.83994	1
TATTAGT	5630	0.0	275.3508	6
GGGGAGC	3710	0.0	275.12128	3
TAGTAGA	5665	0.0	274.95145	9
GGAGCGA	2740	0.0	270.2372	5
GGGAGCG	2775	0.0	267.36035	4
GCGAAAG	2780	0.0	266.8795	8
ATTAGTA	5870	0.0	263.5903	7
CGAAAGC	2810	0.0	263.50534	9
AGCGAAA	2950	0.0	248.0	7
GAGCGAA	2970	0.0	246.32997	6
GGGGGAG	4415	0.0	232.19139	2
GGGAGCA	1310	0.0	201.5458	4
GCAAAAG	1800	0.0	131.93056	8
>>END_MODULE