##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_3522_flu.3520000003bb68.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440633
Sequences flagged as poor quality	0
Sequence length	301
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05573345618689	34.0	34.0	34.0	34.0	34.0
2	33.41645768700937	34.0	34.0	34.0	34.0	34.0
3	33.428597041075	34.0	34.0	34.0	34.0	34.0
4	33.41224783436556	34.0	34.0	34.0	34.0	34.0
5	33.369500241697736	34.0	34.0	34.0	34.0	34.0
6	36.951254218363125	38.0	38.0	38.0	36.0	38.0
7	36.03377186910649	38.0	38.0	38.0	34.0	38.0
8	36.58613857790951	38.0	38.0	38.0	34.0	38.0
9	36.868859118586215	38.0	38.0	38.0	36.0	38.0
10-14	36.87016769057243	38.0	38.0	38.0	36.0	38.0
15-19	36.8327024984511	38.0	38.0	38.0	35.8	38.0
20-24	36.95237851000719	38.0	38.0	38.0	36.0	38.0
25-29	36.86622699616233	38.0	38.0	38.0	36.0	38.0
30-34	36.476916617684104	38.0	37.6	38.0	34.2	38.0
35-39	36.748311179598446	38.0	38.0	38.0	35.6	38.0
40-44	36.27201004010139	38.0	37.2	38.0	33.4	38.0
45-49	36.573062843681704	38.0	37.8	38.0	34.2	38.0
50-54	36.57496465312403	38.0	37.8	38.0	34.4	38.0
55-59	36.210616544834366	38.0	37.0	38.0	33.4	38.0
60-64	36.36500942961602	38.0	37.0	38.0	34.0	38.0
65-69	36.22936593491636	38.0	37.0	38.0	33.4	38.0
70-74	36.10161472245611	38.0	37.0	38.0	32.6	38.0
75-79	36.17162037341733	38.0	37.0	38.0	33.0	38.0
80-84	35.35139447113584	38.0	36.6	38.0	29.0	38.0
85-89	35.61959317618063	38.0	37.0	38.0	30.4	38.0
90-94	35.5530048816135	38.0	37.0	38.0	29.8	38.0
95-99	34.680781965944455	38.0	35.4	38.0	25.0	38.0
100-104	35.00355125467225	38.0	36.0	38.0	26.2	38.0
105-109	34.67842263289404	38.0	35.2	38.0	24.2	38.0
110-114	34.34178738315105	38.0	35.0	38.0	24.0	38.0
115-119	34.317931248907826	38.0	34.8	38.0	23.8	38.0
120-124	33.7626074306736	37.4	33.4	38.0	22.2	38.0
125-129	33.243067586858004	37.0	32.0	38.0	21.6	38.0
130-134	33.08837967197191	37.0	31.8	38.0	21.6	38.0
135-139	32.26316231421614	36.6	29.6	38.0	18.2	38.0
140-144	31.038940796535893	35.4	26.6	38.0	14.8	38.0
145-149	30.789661237356263	35.0	26.4	37.6	14.8	38.0
150-154	29.658045130528127	34.4	23.6	37.0	10.2	38.0
155-159	29.50080815553987	34.2	23.8	37.0	10.0	37.4
160-164	28.44711585378308	32.6	21.4	37.0	10.2	37.2
165-169	27.11095719113185	30.2	20.2	36.2	9.2	37.0
170-174	26.51360746925446	29.2	20.0	35.6	9.0	37.0
175-179	25.60251093313483	27.6	18.0	35.2	9.0	37.0
180-184	24.623984131919308	25.6	16.2	35.0	8.4	36.8
185-189	23.8484802545429	24.8	15.0	34.4	8.0	36.2
190-194	22.720345049054426	23.8	13.2	32.8	8.0	36.0
195-199	21.850001248204286	22.4	11.2	31.4	8.0	35.8
200-204	21.02690402216811	21.0	9.8	29.6	8.0	35.0
205-209	20.00613027167734	20.0	9.0	28.2	8.0	34.8
210-214	18.78632376603659	18.0	9.0	26.4	8.0	34.0
215-219	18.278964126608763	17.0	9.0	25.6	7.6	33.0
220-224	17.562374129944875	16.0	8.8	25.0	7.6	32.0
225-229	16.7612384909891	15.0	8.0	23.6	7.0	31.2
230-234	16.073805638706133	13.8	8.0	22.4	7.0	29.4
235-239	15.1788467954057	12.8	8.0	20.6	7.0	27.8
240-244	14.509820644391137	12.0	8.0	19.6	7.0	26.4
245-249	13.925535763322312	11.8	8.0	18.8	7.0	25.2
250-254	13.337801299494135	11.0	7.8	17.6	7.0	24.6
255-259	12.829449451130534	9.6	8.0	16.2	7.0	23.0
260-264	12.31614291258258	9.0	7.0	15.2	7.0	21.8
265-269	11.95420905833199	9.0	7.0	14.2	7.0	21.0
270-274	11.610872540186506	8.0	7.0	13.8	7.0	20.6
275-279	11.281594887355237	8.0	7.0	13.0	7.0	19.2
280-284	11.137706889860723	8.0	7.0	13.0	7.0	19.0
285-289	10.877995520081337	8.0	7.0	13.0	7.0	19.0
290-294	10.722549604773134	8.0	7.0	13.0	7.0	18.6
295-299	10.490142590318928	8.0	7.0	12.8	7.0	17.8
300-301	10.292975106267573	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2856730111807053
1101	2	0.13027170302866864
1101	3	0.24746285398981627
1101	4	0.24460788621536977
1101	5	0.2745194862259197
1101	6	0.26756023763767445
1101	7	1.0651941136239742
1101	8	0.4102969949638364
1101	9	0.2656333710076737
1101	10-14	0.275664661351108
1101	15-19	0.353231261153212
1101	20-24	0.20212076853688643
1101	25-29	0.1390979388514495
1101	30-34	0.6282472880019938
1101	35-39	0.3319834457062427
1101	40-44	0.5411817195012105
1101	45-49	0.36486639878289395
1101	50-54	0.32893505551651003
1101	55-59	0.39974831690776824
1101	60-64	0.43669322149161616
1101	65-69	0.38482492110451716
1101	70-74	0.31765802024437306
1101	75-79	0.4347606996432205
1101	80-84	0.8458004636393213
1101	85-89	0.4873613237522676
1101	90-94	0.4357526411588424
1101	95-99	1.0515759605964803
1101	100-104	0.5951076778886915
1101	105-109	0.5492157953729802
1101	110-114	0.5415676693045839
1101	115-119	0.6259573074803413
1101	120-124	0.9710892726202047
1101	125-129	0.7439230177601743
1101	130-134	1.0385814661408332
1101	135-139	0.6198235460221397
1101	140-144	1.4889131388682806
1101	145-149	1.2799431814285285
1101	150-154	1.0366390980262175
1101	155-159	0.6040594921550664
1101	160-164	1.1834927157204689
1101	165-169	0.6842424978168538
1101	170-174	0.3460229208721124
1101	175-179	0.7415492194602038
1101	180-184	0.455505823978573
1101	185-189	0.4613983416314831
1101	190-194	0.6345908881477413
1101	195-199	0.7801153904224485
1101	200-204	0.8534011122641552
1101	205-209	0.35925132849944674
1101	210-214	0.6804251212549239
1101	215-219	0.47760371335163043
1101	220-224	0.47399070192808423
1101	225-229	0.46755922694871543
1101	230-234	0.22286998794171353
1101	235-239	0.2916915191009757
1101	240-244	0.119627544939366
1101	245-249	0.1220968214238205
1101	250-254	-0.05688196697104786
1101	255-259	-0.04079357938611672
1101	260-264	0.07516887361442315
1101	265-269	0.02254833698581038
1101	270-274	-0.12620694848649983
1101	275-279	0.16656669401946544
1101	280-284	0.1251740901434335
1101	285-289	0.15642216049275426
1101	290-294	-0.052199812887838704
1101	295-299	-0.07160583720281188
1101	300-301	-0.2914632041168641
1102	1	0.19104468779492123
1102	2	0.09546432375574199
1102	3	0.13738869953150612
1102	4	0.05536932589705401
1102	5	-0.09872160619585202
1102	6	-0.13439309931295185
1102	7	0.9140546670694434
1102	8	0.23088842198500004
1102	9	0.10087482515852031
1102	10-14	0.1179471717508136
1102	15-19	0.11126888097597032
1102	20-24	0.1655174044565797
1102	25-29	0.14206411718387812
1102	30-34	0.461499232295715
1102	35-39	0.2041194558346504
1102	40-44	0.5019702888627648
1102	45-49	0.2074165886906627
1102	50-54	0.17336804520723348
1102	55-59	0.3409564919846346
1102	60-64	0.3018786076373985
1102	65-69	0.17699529549410897
1102	70-74	0.13857319477864394
1102	75-79	0.3874700503395445
1102	80-84	0.8460410134674987
1102	85-89	0.2781082942713411
1102	90-94	0.4021878012239526
1102	95-99	0.8338367672522935
1102	100-104	0.6249846900066132
1102	105-109	0.7451680471364099
1102	110-114	0.8046007675140245
1102	115-119	0.7614827189471569
1102	120-124	1.1719302926961674
1102	125-129	0.8965256583823518
1102	130-134	1.1001857345437998
1102	135-139	1.0641588688653272
1102	140-144	1.6490270835237304
1102	145-149	1.8162010942518236
1102	150-154	1.8810648531356406
1102	155-159	1.2690332667978623
1102	160-164	1.4968206231178307
1102	165-169	1.4779701158309635
1102	170-174	1.5682837275279304
1102	175-179	1.3433614820755047
1102	180-184	1.250302351379549
1102	185-189	1.3409841618521448
1102	190-194	1.5330246012965922
1102	195-199	1.5159374197225048
1102	200-204	1.3999176613688782
1102	205-209	1.119084933129578
1102	210-214	1.7897649657114272
1102	215-219	1.5600942179636768
1102	220-224	1.1531641510147175
1102	225-229	1.0319234881171013
1102	230-234	0.9435283348398862
1102	235-239	0.6158333168944292
1102	240-244	0.6880232765363985
1102	245-249	0.25677397823337067
1102	250-254	0.5436009413306362
1102	255-259	0.564697467819526
1102	260-264	0.5425571897656276
1102	265-269	0.23742444478107316
1102	270-274	0.10629621664281608
1102	275-279	0.16289695265574444
1102	280-284	0.1608695142707628
1102	285-289	0.095090997533811
1102	290-294	0.025434479027515167
1102	295-299	-0.07147561473943931
1102	300-301	-0.16435161070033288
1103	1	-0.15838928565220556
1103	2	-0.04717733105696453
1103	3	0.08634555111993336
1103	4	0.04227959142573923
1103	5	-0.0066637588748506005
1103	6	0.04981446351303731
1103	7	1.064982239378594
1103	8	0.3559091664136389
1103	9	0.25157420875263625
1103	10-14	0.07957649844592396
1103	15-19	0.2295524276805665
1103	20-24	0.1705102920810475
1103	25-29	0.07618870229530472
1103	30-34	0.5608294835996546
1103	35-39	0.1489351267687482
1103	40-44	0.4268102604132693
1103	45-49	0.15274138717335006
1103	50-54	0.17591309025227986
1103	55-59	0.30880144219801053
1103	60-64	0.14083783001767358
1103	65-69	0.16281321868045495
1103	70-74	0.20600029292942423
1103	75-79	0.2223953703964483
1103	80-84	0.6974937161702002
1103	85-89	0.43217301688869725
1103	90-94	0.2411185741021029
1103	95-99	1.0421606174182472
1103	100-104	0.5470081608458699
1103	105-109	0.6957068095830721
1103	110-114	0.5606711800318109
1103	115-119	0.5136128754194189
1103	120-124	0.8510732514443973
1103	125-129	0.9750836242429486
1103	130-134	1.0759485368197588
1103	135-139	1.1408101253850091
1103	140-144	1.5367665335000247
1103	145-149	1.6758792592589344
1103	150-154	2.097868433031323
1103	155-159	0.7200574488746732
1103	160-164	0.7628608080395978
1103	165-169	1.6999171286332384
1103	170-174	2.1192089290452323
1103	175-179	1.8942054839721365
1103	180-184	1.4134815832429943
1103	185-189	1.340869178447683
1103	190-194	1.4700818559196414
1103	195-199	1.5777244040752798
1103	200-204	1.4839551198800187
1103	205-209	1.3513330364961007
1103	210-214	1.6788996266881888
1103	215-219	1.6439331226578417
1103	220-224	1.4129140324751255
1103	225-229	0.8377906052342148
1103	230-234	1.2722291409091113
1103	235-239	0.8544155833714342
1103	240-244	0.6199293931793566
1103	245-249	0.8909228639612046
1103	250-254	0.5393803391495062
1103	255-259	0.7783366333007979
1103	260-264	0.39719682940526724
1103	265-269	0.24780258608026529
1103	270-274	0.32184090606908455
1103	275-279	0.2508456250123121
1103	280-284	0.28437117382505406
1103	285-289	0.0962852839254662
1103	290-294	0.016646546358002112
1103	295-299	0.14383021740849955
1103	300-301	-0.17568844095163705
2116	1	0.21510312740932136
2116	2	0.13245612065413326
2116	3	0.09174173228403504
2116	4	0.16916191316727236
2116	5	0.218915062267115
2116	6	0.19851211594362184
2116	7	1.0849037180244778
2116	8	0.45026450724935785
2116	9	0.3398156071476208
2116	10-14	0.2525696494467624
2116	15-19	0.30164909772945236
2116	20-24	0.23890342924551078
2116	25-29	0.24695304599053713
2116	30-34	0.4897071240387305
2116	35-39	0.13555709429942198
2116	40-44	0.5794357100950265
2116	45-49	0.47433533607105716
2116	50-54	0.3496251555599059
2116	55-59	0.4557274637827291
2116	60-64	0.552361213469382
2116	65-69	0.6264420352102889
2116	70-74	0.5940393345307555
2116	75-79	0.6536205802479316
2116	80-84	0.9792833342866203
2116	85-89	0.6170394959957974
2116	90-94	0.7106854959727755
2116	95-99	1.4025494191892989
2116	100-104	0.6293910129812872
2116	105-109	0.6496749628899607
2116	110-114	0.96055011186467
2116	115-119	0.9227437212823446
2116	120-124	1.2725230715716904
2116	125-129	1.165320341335466
2116	130-134	1.1703707534049457
2116	135-139	1.6427068600035781
2116	140-144	2.113677104480562
2116	145-149	2.020348750080821
2116	150-154	2.261654638178456
2116	155-159	2.3864627620376666
2116	160-164	2.0948141564391882
2116	165-169	1.8055594817371912
2116	170-174	1.7922182224683496
2116	175-179	2.385387109283453
2116	180-184	2.4655547314765514
2116	185-189	1.777573587635029
2116	190-194	1.8700247809265562
2116	195-199	1.3159410123217476
2116	200-204	1.131208602031112
2116	205-209	1.1498058480448528
2116	210-214	1.9986141364197678
2116	215-219	1.8959088398426047
2116	220-224	1.0891051126337814
2116	225-229	0.8178506781057244
2116	230-234	1.1977987378097694
2116	235-239	0.521577284321527
2116	240-244	0.5965413293476072
2116	245-249	0.6278613382716891
2116	250-254	0.670720275502239
2116	255-259	1.3378583563890736
2116	260-264	0.4550259042138638
2116	265-269	0.47534732365059895
2116	270-274	0.48976187738169763
2116	275-279	0.261954494120328
2116	280-284	0.11445373078474219
2116	285-289	0.13853749774337842
2116	290-294	0.25835107084836473
2116	295-299	0.15821542172544234
2116	300-301	-0.15918425545214632
1117	1	0.1782195214307407
1117	2	0.06408198860752634
1117	3	0.06535300685153089
1117	4	0.054703869151737194
1117	5	0.15864379005338947
1117	6	0.1925276508382936
1117	7	0.8871339169182022
1117	8	0.1413996137575353
1117	9	0.2057447586128447
1117	10-14	0.08138334839933492
1117	15-19	0.04316845750349785
1117	20-24	0.15802194166166572
1117	25-29	0.2302159623139488
1117	30-34	0.5367684397972354
1117	35-39	0.24914365426175777
1117	40-44	0.4347016863219224
1117	45-49	0.16258295287698843
1117	50-54	0.20917264290837778
1117	55-59	0.4169002306416303
1117	60-64	0.3055023220959612
1117	65-69	0.37466317208212985
1117	70-74	0.26803780457900217
1117	75-79	0.48376769099289874
1117	80-84	0.7374203238123229
1117	85-89	0.5160756263947519
1117	90-94	0.3470365610545656
1117	95-99	0.9116014375487254
1117	100-104	0.42037488964363945
1117	105-109	0.42372521592044166
1117	110-114	0.5805989526319877
1117	115-119	0.6801669294734722
1117	120-124	0.8516580603912658
1117	125-129	0.9162171284367986
1117	130-134	0.9019189468849973
1117	135-139	1.0761945615453001
1117	140-144	1.244937549343458
1117	145-149	1.70384174760936
1117	150-154	2.2329674544665536
1117	155-159	1.4328626678867913
1117	160-164	1.7681182637711892
1117	165-169	1.4740771938708868
1117	170-174	1.1260483978243627
1117	175-179	1.4077655958081081
1117	180-184	1.1445673241563163
1117	185-189	1.0000373983917648
1117	190-194	-1.0189989921698306
1117	195-199	0.986672446945736
1117	200-204	1.5101293976060184
1117	205-209	1.3011780970739188
1117	210-214	1.471839980835444
1117	215-219	1.4039599166932568
1117	220-224	1.619458766864689
1117	225-229	0.9195671662780178
1117	230-234	1.020449084991128
1117	235-239	0.9766802618430077
1117	240-244	0.9240486848848555
1117	245-249	1.2681502637748103
1117	250-254	0.7427086243312413
1117	255-259	0.6382667358171048
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2108	240-244	-0.5502956813935445
2108	245-249	-0.9951071459818497
2108	250-254	-0.5205949162061838
2108	255-259	-0.5360107408291857
2108	260-264	-0.24208049139379284
2108	265-269	-0.3221660029100626
2108	270-274	-0.4691318183275506
2108	275-279	-0.21321758117045775
2108	280-284	-0.5559772850016671
2108	285-289	-0.9551525041971036
2108	290-294	-0.6551575528917475
2108	295-299	-0.5088950831428871
2108	300-301	2.5401619725889937
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	39.0
11	322.0
12	726.0
13	865.0
14	1216.0
15	1927.0
16	2717.0
17	4221.0
18	5598.0
19	6413.0
20	8353.0
21	11155.0
22	15110.0
23	21260.0
24	30212.0
25	42550.0
26	54389.0
27	62245.0
28	63415.0
29	55027.0
30	34645.0
31	14583.0
32	3311.0
33	313.0
34	14.0
35	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.854781643680795	17.71043022197611	12.731456790571746	33.703331343771346
2	53.030299591723725	20.796899006656332	13.048046787235638	13.124754614384306
3	52.474735210481285	20.00099856343034	15.114619195566378	12.409647030521999
4	52.63359757439865	18.781162554779147	15.416457687009371	13.168782183812834
5	34.59250668924025	18.939344079994008	33.75076310671239	12.71738612405335
6	33.667246892538685	18.729872705857257	33.80704577278597	13.795834628818088
7	15.462981665013743	53.55681485499271	17.03458433662481	13.945619143368745
8	32.697051741471924	17.23157366788234	35.51685870100514	14.554515889640584
9	14.257216322880947	16.297689914282408	36.19497404869813	33.25011971413852
10-14	28.275866764404846	36.748087410611554	20.404282021546276	14.571763803437326
15-19	22.836374034627458	35.88069890362274	18.225280448808874	23.05764661294093
20-24	25.17791450027574	30.242900554429653	24.418280065269737	20.160904880024873
25-29	19.451062448795255	23.35308522058039	39.65731118640683	17.538541144217522
30-34	22.597853542517242	25.90205454425792	29.486216420467827	22.01387549275701
35-39	21.76096660940057	29.511543620200936	26.802123308966873	21.925366461431622
40-44	22.953659848445305	26.620884046360576	27.79732793503891	22.62812817015521
45-49	21.99050003063774	29.186828948353842	25.614604444061158	23.208066576947257
50-54	22.112143212151608	26.89780384129196	28.678560162311946	22.311492784244486
55-59	21.68339638656206	25.02141237719372	29.937703258720976	23.357487977523245
60-64	23.75482544430399	25.84894912546269	26.382136608016193	24.01408882221713
65-69	19.341038914470772	29.043580485347213	27.93031842826116	23.68506217192085
70-74	22.027583045300737	25.071975998166273	28.72944150801234	24.17099944852065
75-79	20.985627494990162	25.592227545372225	27.195103408051597	26.227041551586012
80-84	28.410899773734606	23.24601198730009	26.078800271427692	22.264287967537612
85-89	21.730510424775268	30.44515503831987	23.282414163260583	24.54192037364428
90-94	22.015418727149353	28.995286326716336	27.64150665065939	21.34778829547492
95-99	21.687980700492247	28.904326276061937	25.90632113346027	23.501371889985542
100-104	21.529526839796382	28.574028726854323	24.724566702902415	25.17187773044688
105-109	26.555478141673454	26.288680148785954	22.881808670707823	24.27403303883277
110-114	19.72194547389778	26.81764643138394	30.052810388690816	23.407597706027463
115-119	24.049265488513115	26.540545079465222	23.823544764009956	25.586644668011697
120-124	19.21994040391891	27.20436281440564	27.99663211788495	25.5790646637905
125-129	22.070067380336926	22.68636257384263	30.399357288264838	24.84421275755561
130-134	25.989428844412473	27.494445490918746	23.322674425201924	23.19345123946686
135-139	26.05297378997942	26.94074206879648	23.27056756983703	23.735716571387073
140-144	22.669931666488893	26.57249910923603	25.691448438950324	25.066120785324745
145-149	20.659596534984896	25.376764790653443	28.87337080972147	25.090267864640186
150-154	23.027644320783963	24.412606409415545	25.613333545149818	26.946415724650674
155-159	23.495789012625018	25.297288219447932	27.84244484639144	23.36447792153561
160-164	27.679860564233728	26.324583043031275	23.335610360549484	22.659946032185516
165-169	23.638492804669646	23.809474097491563	26.54599178908525	26.00604130875354
170-174	24.00650881799593	27.70555087794151	24.171317173248486	24.11662313081408
175-179	24.144038235901533	26.7397584838176	25.09044942162752	24.025753858653346
180-184	21.673546919999183	27.505111963924627	25.686637178785976	25.134703937290215
185-189	23.714020511400644	25.363647298318554	26.776206049024925	24.146126141255873
190-194	22.54011842054499	26.050795106131407	25.38774898838716	26.021337484936442
195-199	21.871262479206052	27.37380087283522	27.674822357835204	23.080114290123525
200-204	24.16559812814746	26.982273229649163	24.201092519171283	24.651036123032092
205-209	23.444953056171464	25.681735140127955	26.419038065691858	24.454273738008727
210-214	24.136594399420833	26.831172426940338	25.54397877598818	23.48825439765065
215-219	24.888285716230968	25.77909507458588	26.05878361357411	23.273835595609043
220-224	20.881595341247706	26.712116432495975	27.231324027024755	25.17496419923156
225-229	24.740089825319483	27.095610178992498	23.86993257427383	24.294367421414194
230-234	22.439263514080878	27.058073271861165	26.66364071687776	23.839022497180192
235-239	22.789486942648416	26.39829517988893	23.79553959871367	27.01667827874898
240-244	23.40147015770494	29.090966859041423	22.570029934208286	24.937533049045353
245-249	22.085726670494495	27.797917995247744	23.12999707239358	26.98635826186418
250-254	22.052229406331346	26.476092348961604	23.565416117267656	27.90626212743939
255-259	22.438673453872042	26.78305982529679	25.311131939732157	25.46713478109901
260-264	22.834240740026278	27.852566648435317	23.857268974407273	25.45592363713113
265-269	21.668054821132326	28.047649631325843	23.849825137926572	26.43447040961526
270-274	20.942507710498308	29.24923916274995	23.01461760694274	26.793635519809
275-279	20.841516636293694	29.34314951444853	21.390862690719942	28.424471158537827
280-284	19.326151241509372	29.295808530001153	24.516411616923833	26.861628611565635
285-289	20.386398658293864	28.666940515122562	23.83616297462968	27.11049785195389
290-294	19.847038238170995	29.837211466231533	23.122825571393882	27.19292472420359
295-299	19.107738185746413	31.188994015427806	21.206173845354297	28.49709395347148
300-301	14.723023468510075	32.21637961750481	28.201700735078855	24.858896178906257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.25
25	0.75
26	0.25
27	0.5
28	2.0
29	1.25
30	1.25
31	2.25
32	4.25
33	8.5
34	23.5
35	93.5
36	304.0
37	821.25
38	1807.5
39	3264.5
40	5036.25
41	7342.75
42	10744.0
43	13774.75
44	15654.0
45	14660.5
46	11540.75
47	9040.0
48	7385.25
49	6268.0
50	5426.0
51	4421.75
52	3077.75
53	2123.5
54	1777.0
55	1765.5
56	1947.0
57	2082.0
58	2163.5
59	2326.25
60	2466.75
61	2565.0
62	2363.0
63	1757.5
64	1099.5
65	625.75
66	347.25
67	189.25
68	132.75
69	92.0
70	80.25
71	82.75
72	68.75
73	55.0
74	31.75
75	14.75
76	11.25
77	8.25
78	3.25
79	3.25
80	2.25
81	2.5
82	1.5
83	1.5
84	1.75
85	1.25
86	1.5
87	1.0
88	0.5
89	0.5
90	0.25
91	0.0
92	0.25
93	0.75
94	0.5
95	0.5
96	0.25
97	0.5
98	1.0
99	1.0
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	440633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.945242107388008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.502513514016236	7.600507464141431
2	7.142264389228918	1.99201212205546
3	5.109928790586108	2.13777582408707
4	4.339204263454176	2.4204501602911503
5	3.5844637738324145	2.4993107575627356
6	3.446164137259785	2.883455594153137
7	2.8026823368453417	2.7358858616185797
8	2.354575914471996	2.6268104950029203
9	2.0870366097890507	2.6193807729441527
>10	14.038417066592961	34.53558960497992
>50	0.43701735368355127	4.052287319360702
>100	0.10475593454844886	2.4302816150627025
>500	0.013593577521432386	1.3741145826070118
>1k	0.01869116908479563	6.110651292662013
>5k	0.015292774705741877	16.463309698758
>10k+	0.0033983943790537505	7.518176834713057
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	15990	3.628870284340937	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	15738	3.5716798333306854	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	9794	2.2227114174380946	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9177	2.0826855909566464	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	8990	2.040246645167293	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	8062	1.8296405398597018	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7846	1.7806201532794865	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7763	1.7617836158435702	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	6410	1.454725361014722	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	5797	1.3156073194699442	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5639	1.2797498144714536	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	4527	1.027385602077012	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3995	0.9066502054998151	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3791	0.8603531737296117	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2750	0.6241021439610742	No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG	2268	0.5147140590922605	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1637	0.37151098533246485	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1491	0.33837683514398603	No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC	1444	0.32771036213810584	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT	1369	0.31068939457553113	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG	1289	0.292533695842118	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1227	0.2784630293237229	No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG	902	0.20470550321923234	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	855	0.19403903021335214	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA	727	0.16498991223989123	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	715	0.16226655742987928	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	707	0.16045098755653797	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	692	0.15704679404402302	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	664	0.15069229948732846	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	537	0.12187012774803521	No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT	496	0.11256533214716101	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	446	0.10121802043877785	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	441	0.10008328926793954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.2694623416766335E-4	0.0	0.0
2	2.2694623416766335E-4	0.0	0.0
3	2.2694623416766335E-4	0.0	0.0
4	2.2694623416766335E-4	0.0	0.0
5	2.2694623416766335E-4	0.0	0.0
6	2.2694623416766335E-4	0.0	0.0
7	2.2694623416766335E-4	0.0	0.0
8	2.2694623416766335E-4	0.0	0.0
9	2.2694623416766335E-4	0.0	0.0
10-14	0.0013162881581724475	0.0	0.0
15-19	0.0017701806265077741	0.0	0.0
20-24	0.0018155698733413068	0.0	0.0
25-29	0.002224073094843101	0.0	0.0
30-34	0.005537488113690986	0.0	0.0
35-39	0.006672219284529301	0.0	0.0
40-44	0.008987070873039469	0.0	0.0
45-49	0.010121802043877784	0.0	0.0
50-54	0.012255096645053821	0.0	0.0
55-59	0.01565929015756877	0.0	0.0
60-64	0.01956276538525258	0.0	0.0
65-69	0.027642051321621397	0.0	0.0
70-74	0.0434828984665243	0.0	0.0
75-79	0.06785692401613133	0.0	0.0
80-84	0.10416832148295747	0.0	0.0
85-89	0.15477733170234637	0.0	0.0
90-94	0.2310766556295148	0.0	0.0
95-99	0.3406009082388291	0.0	0.0
100-104	0.4980108162575204	0.0	0.0
105-109	0.6996298506920725	0.0	0.0
110-114	0.9619796973898914	1.3616774050059801E-4	0.0
115-119	1.275437836022268	2.2694623416766335E-4	0.0
120-124	1.660928709379461	2.2694623416766335E-4	0.0
125-129	2.0947591306143662	2.2694623416766335E-4	0.0
130-134	2.605342768244775	2.2694623416766335E-4	0.0
135-139	3.1358068959882717	2.2694623416766335E-4	0.0
140-144	3.7044887695656024	2.2694623416766335E-4	0.0
145-149	4.4519588864202175	2.2694623416766335E-4	0.0
150-154	5.229522073925466	2.2694623416766335E-4	0.0
155-159	6.107486275426489	2.2694623416766335E-4	0.0
160-164	7.016542111008481	2.2694623416766335E-4	0.0
165-169	7.9927286426572675	2.2694623416766335E-4	0.0
170-174	8.988205604210306	2.2694623416766335E-4	0.0
175-179	10.030978160963887	2.2694623416766335E-4	0.0
180-184	11.060315500654742	2.2694623416766335E-4	0.0
185-189	12.126009627059254	2.2694623416766335E-4	0.0
190-194	13.191295250242266	2.2694623416766335E-4	0.0
195-199	14.192037364427994	2.2694623416766335E-4	0.0
200-204	15.121745307319241	2.2694623416766335E-4	1.8155698733413067E-4
205-209	16.00710795605413	2.2694623416766335E-4	4.538924683353267E-4
210-214	16.81848613245036	2.2694623416766335E-4	4.538924683353267E-4
215-219	17.565366189096142	2.2694623416766335E-4	4.992817151688594E-4
220-224	18.280791497686284	2.2694623416766335E-4	6.8083870250299E-4
225-229	18.887328003122782	2.2694623416766335E-4	6.8083870250299E-4
230-234	19.425962195296314	2.2694623416766335E-4	6.8083870250299E-4
235-239	19.89437922261837	2.2694623416766335E-4	6.8083870250299E-4
240-244	20.30683130859468	2.2694623416766335E-4	6.8083870250299E-4
245-249	20.57390163696319	2.2694623416766335E-4	6.8083870250299E-4
250-254	20.81873123438326	2.2694623416766335E-4	6.8083870250299E-4
255-259	21.021621167729155	2.2694623416766335E-4	6.8083870250299E-4
260-264	21.17839562629218	2.2694623416766335E-4	6.8083870250299E-4
265-269	21.301309706717383	2.2694623416766335E-4	6.8083870250299E-4
270-274	21.406158866902842	2.2694623416766335E-4	6.8083870250299E-4
275-279	21.470566208159624	2.2694623416766335E-4	6.8083870250299E-4
280-284	21.51432144210715	2.2694623416766335E-4	6.8083870250299E-4
285-289	21.544187566523615	2.2694623416766335E-4	6.8083870250299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7775	0.0	289.3087	1
GGGTTAT	7850	0.0	287.29617	2
GTTATTA	7825	0.0	286.51755	4
GGTTATT	7865	0.0	286.37317	3
TTATTAG	7925	0.0	281.7855	5
TTAGTAG	8065	0.0	279.27155	8
GGGGGGA	8370	0.0	277.90622	1
TATTAGT	8110	0.0	274.99384	6
GGGGAGC	8570	0.0	271.7649	3
GGAGCGA	6705	0.0	271.02164	5
ATTAGTA	8245	0.0	270.67007	7
GGGAGCG	6775	0.0	268.87454	4
CGAAAGC	6865	0.0	267.49817	9
GCGAAAG	6990	0.0	262.9256	8
GAGCGAA	6950	0.0	262.1043	6
TAGTAGA	8610	0.0	261.25143	9
AGCGAAA	7065	0.0	257.83795	7
GGGGGAG	9230	0.0	253.45068	2
GGGAGCA	2145	0.0	234.48718	4
CAAAAGC	2660	0.0	183.54324	9
>>END_MODULE