##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30515_flu.3520000003ba90.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385756
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03837659038356	34.0	34.0	34.0	34.0	34.0
2	33.302958346726946	34.0	34.0	34.0	34.0	34.0
3	33.3043841184583	34.0	34.0	34.0	34.0	34.0
4	33.456591731560884	34.0	34.0	34.0	34.0	34.0
5	33.33183670506745	34.0	34.0	34.0	34.0	34.0
6	36.85282665726521	38.0	38.0	38.0	36.0	38.0
7	35.937253082259254	38.0	38.0	38.0	33.0	38.0
8	36.72573336513236	38.0	38.0	38.0	34.0	38.0
9	36.88369072678066	38.0	38.0	38.0	36.0	38.0
10-14	36.90169277989195	38.0	38.0	38.0	36.0	38.0
15-19	36.685760428872136	38.0	38.0	38.0	35.4	38.0
20-24	36.73484689803917	38.0	38.0	38.0	35.2	38.0
25-29	36.679437779321646	38.0	38.0	38.0	35.2	38.0
30-34	36.29839069256214	38.0	37.4	38.0	33.0	38.0
35-39	36.45877705077821	38.0	37.8	38.0	34.0	38.0
40-44	35.95322328103775	38.0	37.0	38.0	32.0	38.0
45-49	36.349065730669125	38.0	37.0	38.0	34.0	38.0
50-54	36.32296114642416	38.0	37.0	38.0	34.0	38.0
55-59	35.907401569904295	38.0	37.0	38.0	32.4	38.0
60-64	36.05655129148995	38.0	37.0	38.0	32.6	38.0
65-69	35.88243345534483	38.0	37.0	38.0	32.0	38.0
70-74	35.682967471666025	38.0	37.0	38.0	31.2	38.0
75-79	35.702227314675596	38.0	37.0	38.0	31.0	38.0
80-84	34.97002509358247	38.0	36.0	38.0	27.0	38.0
85-89	35.14315733261439	38.0	36.0	38.0	27.4	38.0
90-94	35.140107736496645	38.0	36.2	38.0	27.2	38.0
95-99	34.082961768579096	37.8	34.8	38.0	22.6	38.0
100-104	34.34117887991373	38.0	35.0	38.0	24.0	38.0
105-109	34.224376030444105	37.8	34.8	38.0	23.6	38.0
110-114	33.739569054013415	37.0	34.0	38.0	23.0	38.0
115-119	33.726220201370815	37.0	34.0	38.0	23.0	38.0
120-124	33.015650825910676	37.0	32.4	38.0	20.6	38.0
125-129	32.54495017575877	37.0	31.0	38.0	19.2	38.0
130-134	32.53572050726366	37.0	31.0	38.0	19.2	38.0
135-139	32.006152593867625	36.8	28.6	38.0	17.0	38.0
140-144	30.451674115243833	35.4	25.4	37.6	11.6	38.0
145-149	30.15833480231027	35.0	24.2	37.4	10.8	38.0
150-154	29.372033617105114	34.0	23.4	37.0	10.0	38.0
155-159	28.81040605978909	33.8	22.4	37.0	10.0	37.0
160-164	28.27685220709464	32.4	21.4	37.0	10.0	37.2
165-169	26.927520505189808	30.0	20.0	35.8	9.2	37.0
170-174	26.437501425771735	28.8	19.6	35.6	9.0	37.0
175-179	25.785714285714285	28.2	18.2	35.2	9.0	37.0
180-184	24.44584556040606	25.8	16.0	35.0	8.2	36.6
185-189	23.804037267080748	25.0	15.0	35.0	8.0	36.2
190-194	22.87041445888074	23.6	13.4	33.6	8.0	36.0
195-199	21.93635873453686	22.6	10.6	32.4	8.0	36.0
200-204	21.094851667893693	21.2	9.2	30.0	8.0	35.2
205-209	20.5338084177563	20.2	9.0	28.8	8.0	35.0
210-214	19.355499849645888	18.6	9.0	26.8	8.0	35.0
215-219	18.910711434170825	18.2	9.0	26.4	8.0	34.6
220-224	18.251790250832133	17.0	9.0	26.0	7.8	34.0
225-229	17.55883304472257	15.8	8.6	25.0	7.2	32.2
230-234	17.003725152687192	15.0	8.0	24.2	7.0	31.6
235-239	16.215878949387694	14.0	8.0	22.8	7.0	30.0
240-244	15.604474849386659	13.0	8.0	21.6	7.0	28.6
245-249	14.966438888831282	12.2	8.0	20.4	7.0	27.4
250-254	14.237884569520627	11.6	8.0	19.2	7.0	25.8
255-259	13.625515610904301	11.0	8.0	18.2	7.0	25.0
260-264	13.299902529059818	10.6	8.0	17.4	7.0	24.4
265-269	12.882581217142441	9.4	8.0	16.0	7.0	23.2
270-274	12.366375117950206	9.0	7.2	15.2	7.0	22.2
275-279	11.977378446479122	9.0	7.0	14.4	7.0	21.0
280-284	11.705341200136875	9.0	7.0	13.8	7.0	20.6
285-289	11.442847810533085	8.8	7.0	13.4	7.0	19.6
290-294	11.307546739389668	8.0	7.0	13.0	7.0	19.4
295-299	10.874261968705607	8.0	7.0	13.0	7.0	18.8
300-301	10.63752734889412	8.0	7.0	13.0	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.15422759383236695
1101	2	0.06419038607005945
1101	3	0.09124034063693642
1101	4	0.015086729762387563
1101	5	0.20168167724092
1101	6	0.1336935863123827
1101	7	1.064188520755586
1101	8	0.3154672405479033
1101	9	0.21529044604827163
1101	10-14	0.16250065715783535
1101	15-19	0.15258669001499925
1101	20-24	0.1323279710490155
1101	25-29	0.13842411841175561
1101	30-34	0.5415099093822846
1101	35-39	0.31416221384079535
1101	40-44	0.5413561282878945
1101	45-49	0.16242488026571067
1101	50-54	0.2903547152579975
1101	55-59	0.33073818769988605
1101	60-64	0.19451270184519132
1101	65-69	0.3784530113789799
1101	70-74	0.2808222168841823
1101	75-79	0.3859119240214923
1101	80-84	0.8732364429948021
1101	85-89	0.4770021252951082
1101	90-94	0.32189201111363985
1101	95-99	0.9618598777470879
1101	100-104	0.3485568173193343
1101	105-109	0.5213626106204146
1101	110-114	0.5115282214410399
1101	115-119	0.43729301009519617
1101	120-124	0.8874612892356168
1101	125-129	0.5143338026665276
1101	130-134	0.7820227266751729
1101	135-139	0.4756142503273679
1101	140-144	1.1772734337173922
1101	145-149	1.0263760710770526
1101	150-154	0.8482158465567977
1101	155-159	0.8256489239143967
1101	160-164	1.149993713025335
1101	165-169	0.6662867784829061
1101	170-174	0.5457581612611904
1101	175-179	0.634324538385659
1101	180-184	0.6652326958780712
1101	185-189	0.36095015159187227
1101	190-194	0.6402073087150946
1101	195-199	0.6959397492074118
1101	200-204	0.989914570688196
1101	205-209	0.6068716027572059
1101	210-214	0.6445351960840675
1101	215-219	0.677590893384739
1101	220-224	0.5405802652461773
1101	225-229	0.33804265940438327
1101	230-234	0.21908833691842844
1101	235-239	0.26620615624088373
1101	240-244	0.3047308844477623
1101	245-249	0.10326655960452236
1101	250-254	0.3807337675749771
1101	255-259	0.16926165759053546
1101	260-264	0.09598743572362345
1101	265-269	0.1432248889384926
1101	270-274	0.021114272283034197
1101	275-279	0.1112753129940316
1101	280-284	0.05910383540688713
1101	285-289	-0.07512969093134103
1101	290-294	-0.15579987080579194
1101	295-299	-0.144001908035726
1101	300-301	-0.3239638380192158
1102	1	0.005133920802812497
1102	2	0.040732681328677245
1102	3	0.11449503583398268
1102	4	0.05277530734859681
1102	5	0.18474336905126165
1102	6	0.13249188526558697
1102	7	1.2385121429846464
1102	8	0.4024698190577567
1102	9	0.26632473638078125
1102	10-14	0.20830230432532204
1102	15-19	0.33660955023667327
1102	20-24	0.32531134543324924
1102	25-29	0.22072630437234153
1102	30-34	0.5383198693576503
1102	35-39	0.32901423816345954
1102	40-44	0.6418312283494672
1102	45-49	0.326042491103145
1102	50-54	0.22949918878016717
1102	55-59	0.3591718391777192
1102	60-64	0.40065648263337295
1102	65-69	0.3460588457385896
1102	70-74	0.22760696903934985
1102	75-79	0.4447950828884899
1102	80-84	0.7985075713814993
1102	85-89	0.37846263945766623
1102	90-94	0.4426086059412313
1102	95-99	1.0099706382150728
1102	100-104	0.7706619589449488
1102	105-109	0.7327592393149942
1102	110-114	0.7727287679311914
1102	115-119	0.7216449189621912
1102	120-124	1.0345341032750213
1102	125-129	0.7233245277280957
1102	130-134	1.019861011773699
1102	135-139	1.1565629110416538
1102	140-144	1.6077048530523648
1102	145-149	1.936123800448975
1102	150-154	1.485916162135613
1102	155-159	1.204510531057256
1102	160-164	1.4851457296509558
1102	165-169	1.4537989013277297
1102	170-174	1.443247962677443
1102	175-179	1.1624193274866457
1102	180-184	1.488347304868217
1102	185-189	1.2840332027002468
1102	190-194	1.5843964250944005
1102	195-199	1.5392319674955885
1102	200-204	1.883129048828593
1102	205-209	1.3331035147030157
1102	210-214	1.602835496268792
1102	215-219	1.334539785010353
1102	220-224	1.086579726453074
1102	225-229	0.8816063909314806
1102	230-234	1.0313535770662092
1102	235-239	0.7830649924477804
1102	240-244	0.9647201085856949
1102	245-249	0.46520794661191367
1102	250-254	0.6043001930675871
1102	255-259	0.5089841791422636
1102	260-264	0.6307796155265795
1102	265-269	0.37011509060105396
1102	270-274	0.4380258332436249
1102	275-279	0.38890885671324504
1102	280-284	0.36624361373201
1102	285-289	0.3590274827139801
1102	290-294	0.16700127605381176
1102	295-299	0.1347696436884096
1102	300-301	-0.07247638420148128
1103	1	-0.02840665980841095
1103	2	0.11261081992928013
1103	3	0.1617235020447012
1103	4	0.07932368364588172
1103	5	-0.044148495695971235
1103	6	0.03286493036578264
1103	7	1.2108302834983036
1103	8	0.4172098831449915
1103	9	0.192442676024001
1103	10-14	0.18315523846851534
1103	15-19	0.23816390482082284
1103	20-24	0.21337772833057045
1103	25-29	0.14700876647000882
1103	30-34	0.6282658256444194
1103	35-39	0.34116881274857036
1103	40-44	0.5709469201568282
1103	45-49	0.2525401715278406
1103	50-54	0.23052841064634322
1103	55-59	0.42144358818532623
1103	60-64	0.4421917066995604
1103	65-69	0.4910614761848109
1103	70-74	0.4122872502579682
1103	75-79	0.5692993633418837
1103	80-84	0.9466667372909185
1103	85-89	0.46963486194559323
1103	90-94	0.5733953484922836
1103	95-99	1.1266285367276794
1103	100-104	0.7435492323678901
1103	105-109	0.8960114273679878
1103	110-114	0.9542993834555986
1103	115-119	0.9811006557262587
1103	120-124	1.4410671751579542
1103	125-129	1.3778771127393412
1103	130-134	1.4117276720635275
1103	135-139	1.5617224117351398
1103	140-144	1.9762456727950628
1103	145-149	2.161201996441129
1103	150-154	2.5947104612412666
1103	155-159	1.287173499157113
1103	160-164	1.2593922263748496
1103	165-169	1.7867885230217446
1103	170-174	2.057180718906821
1103	175-179	2.071466149429348
1103	180-184	1.6305004446644809
1103	185-189	1.4686981795044893
1103	190-194	1.6229568536180068
1103	195-199	1.7877574828239169
1103	200-204	1.6668642824600397
1103	205-209	1.2333923855241977
1103	210-214	1.7173670377102823
1103	215-219	2.04820375746241
1103	220-224	1.3386453441296737
1103	225-229	0.9084522467830247
1103	230-234	1.5058525966271645
1103	235-239	1.1601791538136972
1103	240-244	0.9730227533798015
1103	245-249	1.1647095966190868
1103	250-254	0.7851868925749788
1103	255-259	0.8119034961827705
1103	260-264	0.6260754211605164
1103	265-269	0.5979863422151936
1103	270-274	0.6122694603898307
1103	275-279	0.44765198168335374
1103	280-284	0.2726657286107699
1103	285-289	0.39149197169001937
1103	290-294	0.20695536281071192
1103	295-299	0.03544590749825538
1103	300-301	-0.2772064047667886
2116	1	-0.09177993375880078
2116	2	0.014880540220730154
2116	3	-0.006847021407210718
2116	4	0.17582779262227177
2116	5	0.21068817016318775
2116	6	0.19745505764155524
2116	7	1.1007749381020346
2116	8	0.5087347699384992
2116	9	0.36250771152332106
2116	10-14	0.18958573993135275
2116	15-19	0.30405846904570666
2116	20-24	0.24484596529085678
2116	25-29	0.2347068079511061
2116	30-34	0.5104885862057031
2116	35-39	0.12668658104328756
2116	40-44	0.6469564611813752
2116	45-49	0.2553771355440162
2116	50-54	0.3428851555171164
2116	55-59	0.42160161140429864
2116	60-64	0.5297604225745545
2116	65-69	0.5901230269739983
2116	70-74	0.6718269703630639
2116	75-79	0.5546904149044067
2116	80-84	1.198092114180021
2116	85-89	0.8486289547096888
2116	90-94	0.6799969175435905
2116	95-99	1.5151565728046705
2116	100-104	0.912018009737757
2116	105-109	0.8281426909946958
2116	110-114	1.042970534900725
2116	115-119	0.9303502317842529
2116	120-124	1.202281046913157
2116	125-129	1.2102539832087515
2116	130-134	1.149381921732747
2116	135-139	1.4388332702409983
2116	140-144	2.2573350639957077
2116	145-149	2.154026847828497
2116	150-154	2.4100856732122686
2116	155-159	2.4380394589431873
2116	160-164	1.9018568668161215
2116	165-169	1.9339934873360782
2116	170-174	1.607078413780382
2116	175-179	2.2778634809240437
2116	180-184	2.5035026994769183
2116	185-189	1.7898766451139458
2116	190-194	1.9157313910087588
2116	195-199	0.9430767903542474
2116	200-204	0.8081243685528783
2116	205-209	1.1862854403291827
2116	210-214	1.532299486511132
2116	215-219	2.077776833883778
2116	220-224	1.2828269962922434
2116	225-229	1.2418484415540973
2116	230-234	1.3974967462274464
2116	235-239	0.9515963313848381
2116	240-244	1.0596463115110986
2116	245-249	0.9939181760872486
2116	250-254	1.1653404434386996
2116	255-259	1.6962897585982155
2116	260-264	0.776958524975063
2116	265-269	0.7115673943367646
2116	270-274	0.6849046093751632
2116	275-279	0.7441005589153367
2116	280-284	0.4670752845431636
2116	285-289	0.20957370398228647
2116	290-294	0.4715644975963187
2116	295-299	0.2505218443635062
2116	300-301	0.06853181337233849
1117	1	0.1988472222603761
1117	2	0.13379222403528956
1117	3	0.19231886353115613
1117	4	0.18983498514926112
1117	5	0.2071689828848946
1117	6	0.33930255576635204
1117	7	1.0778804817619516
1117	8	0.4340789340469229
1117	9	0.3105564612906804
1117	10-14	0.3299471709923125
1117	15-19	0.31921522515210654
1117	20-24	0.3516809338306004
1117	25-29	0.3787560540141115
1117	30-34	0.6785527136183234
1117	35-39	0.44432049157097
1117	40-44	0.7378896661796688
1117	45-49	0.4694676079836313
1117	50-54	0.47645711180549455
1117	55-59	0.4608916830475849
1117	60-64	0.5431369060567448
1117	65-69	0.6804183792781018
1117	70-74	0.6490842225771374
1117	75-79	0.6466910315141519
1117	80-84	1.0005463337269234
1117	85-89	0.7500620083754157
1117	90-94	0.7203011994094268
1117	95-99	1.2054878313465025
1117	100-104	0.7772335129608621
1117	105-109	0.6763634523197268
1117	110-114	1.017672052550246
1117	115-119	1.0936486051000927
1117	120-124	1.3418128746910583
1117	125-129	1.2859059822086465
1117	130-134	1.2096446200395263
1117	135-139	1.4208192423694825
1117	140-144	1.7016899830562728
1117	145-149	2.340641152798412
1117	150-154	2.5519918244583657
1117	155-159	2.193244265491284
1117	160-164	2.1273294286227866
1117	165-169	1.8590797281033744
1117	170-174	1.525109172830728
1117	175-179	2.0253843525996658
1117	180-184	1.9481779548153924
1117	185-189	1.5355924293832537
1117	190-194	-0.4269974078617942
1117	195-199	1.4698024986564846
1117	200-204	1.7243620716064143
1117	205-209	1.6413885438688638
1117	210-214	2.175672905743717
1117	215-219	1.691630437948895
1117	220-224	1.6614387220287412
1117	225-229	1.1944961976022306
1117	230-234	1.0584775692886517
1117	235-239	0.9320278078079731
1117	240-244	0.5989813129492312
1117	245-249	0.9152712960761189
1117	250-254	0.614138900410433
1117	255-259	0.8332572042359825
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2108	240-244	-0.7012546924753167
2108	245-249	-0.9302941375108631
2108	250-254	-0.600390030501945
2108	255-259	-0.581645794332541
2108	260-264	-0.49711352581483936
2108	265-269	-0.3657384524076619
2108	270-274	-0.5857066411785041
2108	275-279	-0.35989656200596976
2108	280-284	-0.9454634722854198
2108	285-289	-1.2224494024698025
2108	290-294	-0.934612971767331
2108	295-299	-0.5102038311126478
2108	300-301	2.518433998519246
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	16.0
11	273.0
12	834.0
13	1125.0
14	1565.0
15	2265.0
16	3217.0
17	4786.0
18	6051.0
19	6699.0
20	8349.0
21	10730.0
22	13961.0
23	18256.0
24	24183.0
25	32229.0
26	41078.0
27	48029.0
28	50362.0
29	47224.0
30	36260.0
31	20344.0
32	6889.0
33	977.0
34	46.0
35	5.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.027094847520196	18.98661329959871	13.994856852518172	33.99143500036293
2	32.88114766847437	22.230114372815976	14.237756509296032	30.650981449413617
3	50.50057549331702	21.763498169827557	16.025415029189436	11.710511307665985
4	51.13284044836632	20.725795580626095	16.213616897728098	11.927747073279482
5	49.616078557430086	20.778937981522	17.45844523481164	12.146538226236274
6	29.862400066363193	21.080164663673408	35.68214104252429	13.375294227439108
7	16.251205425191053	34.76290712263711	35.764317340495026	13.221570111676812
8	28.969089268864256	36.90519395680171	19.370016279720858	14.755700494613174
9	13.94378830141333	18.81785377285123	38.34496417424486	28.89339375149058
10-14	24.768091747114756	35.4937836352513	25.640871431682204	14.097253185951741
15-19	25.590580574249007	33.17397525897199	19.85851159800496	21.376932568774045
20-24	24.212714773068985	28.86705585914412	24.320606808448865	22.59962255933803
25-29	19.27798919524259	26.54543286429764	32.93470483932849	21.241873101131283
30-34	20.26623046692728	28.544468524144794	30.329534731799374	20.859766277128546
35-39	23.3576665042151	28.31707089455511	28.342320015761256	19.982942585468535
40-44	20.543193106523294	26.938375553458666	30.65243314426736	21.865998195750684
45-49	21.49975632264955	28.521604330198365	29.151795435456613	20.826843911695477
50-54	19.310652329451777	29.952768071008617	30.249017513661485	20.487562085878118
55-59	22.39819989838136	27.517394415122514	25.235693028753925	24.8487126577422
60-64	19.9278818735159	28.640954385673844	28.663040885948632	22.768122854861623
65-69	20.09513785916486	28.147067057932993	28.2524704735636	23.505324609338548
70-74	20.20878482771493	28.437924491129106	26.283557481931584	25.06973319922438
75-79	18.452130362197867	34.282292433559036	25.756540403778555	21.509036800464543
80-84	21.153527100032143	29.170149006107486	24.665280643722976	25.011043250137394
85-89	22.728356785118052	28.667655201733737	25.118883439272494	23.485104573875716
90-94	21.006387457356464	28.68253507398459	27.324733769533072	22.98634369912587
95-99	23.05965428923983	26.803471624550234	26.556579806924585	23.58029427928535
100-104	22.155507626582608	26.369051939568017	28.333091384191043	23.142349049658336
105-109	23.117981314613385	25.438930308277776	30.492435632887112	20.95065274422174
110-114	21.354016528582832	27.544821078609278	29.103837659038355	21.99732473376953
115-119	19.58839266271944	29.63241012453468	27.03071371540559	23.748483497340285
120-124	24.203693526477878	28.28917761486536	27.01655969058161	20.490569168075158
125-129	22.650483725463765	28.33656508259107	27.642395711278635	21.370555480666535
130-134	22.84096682877259	26.241095407459635	28.777361855680795	22.140575908086976
135-139	20.643930360124017	24.963396551187795	30.736579599539603	23.65609348914858
140-144	20.563879758189113	27.727371706467302	26.992140109291885	24.7166084260517
145-149	20.816682047719283	29.242007901367707	27.486908823193936	22.454401227719075
150-154	20.85867750598824	26.07513557793009	28.487126577421996	24.57906033865967
155-159	22.434025653521918	29.24247451757069	27.727682783935958	20.595817044971433
160-164	21.137299225417102	25.713508020614068	28.73572932112527	24.41346343284356
165-169	23.558933626437437	26.536463463951304	26.355105299723142	23.549497609888114
170-174	18.57319134375097	27.988676780140814	31.201381183960848	22.236750692147368
175-179	20.15320565331453	26.589502172357655	30.02084219040015	23.236449983927663
180-184	23.340660935928412	26.858947106461077	25.30070821970365	24.499683737906864
185-189	21.55087671999917	28.862182312135133	26.46128640902539	23.125654558840303
190-194	20.505345347836457	27.32727423552712	26.997687657482967	25.169692759153456
195-199	22.00183535706509	27.17894212922158	29.497817273094913	21.32140524061842
200-204	21.688684038615083	26.421986955484815	27.692479183732722	24.19684982216738
205-209	22.679413930049048	25.938883646657473	27.876533352689265	23.505169070604214
210-214	21.14564647082612	27.41753854768299	26.982289322784354	24.45452565870654
215-219	22.421167784817346	28.256151556942733	25.548170346021838	23.774510312218087
220-224	21.216727672414688	26.69666836031066	28.684038615083107	23.40256535219154
225-229	21.26230052157322	27.505884548782134	25.715737409139454	25.51607752050519
230-234	19.721533819305467	27.227625753066704	25.761413950787542	27.289426476840283
235-239	20.872987069546554	27.62279783075312	25.22921224815687	26.275002851543462
240-244	19.89366335196342	26.62128392040565	26.91431889588237	26.570733831748566
245-249	21.563579049969412	26.449672850195462	26.05123445908813	25.935513640746997
250-254	21.431837741992346	26.82659504972055	25.091508622030506	26.6500585862566
255-259	20.10389987453209	28.7089766588206	24.224691255612356	26.962432211034955
260-264	20.507367351382737	29.232416346084054	24.435861010587004	25.824355291946205
265-269	20.979168178848806	27.87539273530418	24.273893341905247	26.871545743941766
270-274	19.891226578458923	27.37092877362893	25.43213845021205	27.305706197700104
275-279	20.935928410705213	28.691815551799582	24.53385041321457	25.838405624280636
280-284	19.597050985597114	27.688487022884935	25.84675286968965	26.867709121828305
285-289	20.254098445649582	28.791360341770446	23.881520961436763	27.07302025114321
290-294	18.88971266811145	29.064227128029117	23.221362726697706	28.82469747716173
295-299	19.297275998299444	28.864982009353064	23.53124773172679	28.306494260620703
300-301	15.802994639098289	27.163932641358784	33.127287715550764	23.90578500399216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.5
21	1.0
22	0.25
23	0.75
24	1.75
25	1.5
26	3.0
27	4.25
28	7.5
29	17.0
30	52.25
31	184.5
32	527.25
33	1215.75
34	2376.75
35	3928.75
36	5143.0
37	5580.25
38	6090.75
39	6966.25
40	8125.5
41	8650.5
42	8425.5
43	7789.25
44	7231.25
45	6523.75
46	5239.75
47	3845.75
48	2775.75
49	2264.5
50	2203.5
51	2215.75
52	2214.5
53	2561.0
54	3165.75
55	3560.25
56	3585.75
57	3556.25
58	3267.25
59	2538.25
60	1681.75
61	1114.5
62	826.5
63	679.5
64	523.5
65	384.0
66	286.25
67	238.0
68	213.75
69	193.25
70	178.0
71	139.0
72	102.0
73	65.75
74	47.5
75	34.0
76	16.0
77	14.5
78	11.0
79	9.0
80	7.0
81	4.5
82	3.5
83	2.25
84	1.75
85	1.0
86	0.75
87	0.75
88	1.25
89	1.25
90	1.0
91	0.5
92	0.5
93	0.5
94	0.75
95	0.25
96	0.25
97	0.25
98	0.5
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	385756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.85501324692282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.670962493375356	11.062417750223043
2	7.68670282161373	2.898657670533718
3	4.864757354555638	2.7517519308963534
4	4.172894886243539	3.1471995343255377
5	3.6318910836106464	3.423967724642981
6	3.138285691348102	3.5503450969798083
7	2.820155312146175	3.7221846038227118
8	2.1432601793974833	3.232895925931339
9	2.015351967427521	3.419953923875209
>10	10.33271006872376	31.47553834264408
>50	0.3059293430332302	4.000978741165158
>100	0.1474210505997428	5.444315335193997
>500	0.034127876551856895	4.52555221841348
>1k	0.031283886808349856	10.508322054825536
>5k	0.0028439897098499864	4.066267248832293
>10k+	0.0014219948549249932	2.769651897694685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT	10330	2.677858542705752	No Hit
CCGGGTTATTAGTAGAAACACGAGCAATTAGTATGGGTTAGCTTCACGCG	9872	2.559130642167588	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	5294	1.3723700992337127	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	3324	0.8616845881852778	No Hit
CCGGGTTATTAGTAGAAACACGTGCAATTAGTATGGGTTAGCTTCACGCG	2968	0.7693982724831241	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	2958	0.7668059602442995	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	2681	0.6949989112288597	No Hit
CCGGGTTATTAGTAGAAACACGAGCGTGCCAAACAATTCCTGCAACAGGT	2497	0.647300366034488	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2408	0.6242287871089497	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	2272	0.588973340660936	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	2017	0.5228693785709102	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	1962	0.5086116612573751	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA	1881	0.48761393212289633	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	1446	0.3748483497340288	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	1282	0.3323344290173063	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1236	0.3204097927187134	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT	1229	0.3185951741515362	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1224	0.31729901803212396	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1181	0.3061520754051784	No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT	1160	0.30070821970364686	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	1139	0.2952643640021153	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1137	0.29474590155435043	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1099	0.28489511504681714	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	1057	0.2740074036437541	No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT	1035	0.2683043167183401	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	990	0.2566389116436296	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	927	0.24030734453903504	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	915	0.23719656985244558	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	863	0.22371654621055798	No Hit
GGGGGGAGCAGAAGCGGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT	814	0.21101421624031771	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCTGATACCTCCAGCACACTTTA	786	0.203755741971609	No Hit
CCGGGTTATTAGTAGAAACACGTGCGTGCCAAACAATTCCTGCAACAGGT	779	0.20194112340443182	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	773	0.2003857360611371	No Hit
CCGGGTTATTAGTAGAAACACGAGCGGTGTCCCTGGACCAGATGCTCGCC	756	0.19597880525513536	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	735	0.19053494955360384	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	708	0.18353570650877757	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	683	0.1770549259117162	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	659	0.1708333765385373	No Hit
CCGGGTTATTAGTAGAAACACGAGCCAACCTACCCGAACAGCGAGCCACA	658	0.17057414531465487	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	632	0.16383413349371106	No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT	624	0.16176028370265141	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA	614	0.15916797146382688	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	596	0.15450180943394268	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	590	0.15294642209064796	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	589	0.15268719086676552	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	580	0.15035410985182343	No Hit
GGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAATA	566	0.14672487271746906	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCAATTATTAAAGCAACA	531	0.13765177988158317	No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGCA	511	0.1324671554039341	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	480	0.12443098746357802	No Hit
CCGGGTTATTAGTAGTAACAAGAGCAATTAGTATGGGTTAGCTTCACGCG	447	0.11587635707545703	No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTA	425	0.11017327015004302	No Hit
GGGGGGAGCAGAAGCGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCC	409	0.10602557056792376	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	397	0.10291479588133431	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	5.184624477649084E-4	0.0	0.0
7	7.776936716473626E-4	0.0	0.0
8	7.776936716473626E-4	0.0	0.0
9	0.0010369248955298168	0.0	0.0
10-14	0.0017627723224006887	0.0	0.0
15-19	0.002384927259718579	0.0	0.0
20-24	0.0034736984000248864	0.0	0.0
25-29	0.004510623295554702	0.0	0.0
30-34	0.008036167940356078	0.0	0.0
35-39	0.01104325013739255	0.0	0.0
40-44	0.05780856292578729	0.0	0.0
45-49	0.12526052738000187	0.0	0.0
50-54	0.1312746917740748	0.0	0.0
55-59	0.1397774759174193	0.0	0.0
60-64	0.15242795964288308	0.0	0.0
65-69	0.1737367662460208	0.0	0.0
70-74	0.20111158348800795	0.0	0.0
75-79	0.2359004137330333	0.0	0.0
80-84	0.2895612770767014	0.0	0.0
85-89	0.3768185070355354	0.0	0.0
90-94	0.43566399485685253	0.0	0.0
95-99	0.5108410497827642	0.0	0.0
100-104	0.5938468876699261	0.0	0.0
105-109	0.6931842926616824	0.0	0.0
110-114	0.8046537189311378	0.0	0.0
115-119	0.9282033202335155	0.0	0.0
120-124	1.0475533757089974	0.0	0.0
125-129	1.169495743423304	0.0	0.0
130-134	1.301237051400367	0.0	0.0
135-139	1.4314229720341356	0.0	0.0
140-144	1.6180176069847263	0.0	0.0
145-149	1.8585841827476437	0.0	0.0
150-154	2.105268615394187	0.0	0.0
155-159	2.6181316687232346	0.0	0.0
160-164	2.9024046288327336	0.0	0.0
165-169	3.42164477026929	0.0	0.0
170-174	3.9085328549653147	0.0	0.0
175-179	4.209033689689856	0.0	0.0
180-184	4.498283889297898	0.0	0.0
185-189	4.830514625825651	0.0	0.0
190-194	5.163263824801169	0.0	0.0
195-199	5.519862296373875	0.0	0.0
200-204	5.875423843051047	0.0	2.0738497910596337E-4
205-209	6.249287114134323	0.0	2.592312238824542E-4
210-214	6.653117514698411	0.0	2.592312238824542E-4
215-219	7.06088822986551	0.0	2.592312238824542E-4
220-224	7.474206493223695	0.0	2.592312238824542E-4
225-229	7.862897790313047	0.0	2.592312238824542E-4
230-234	8.229087817169402	0.0	2.592312238824542E-4
235-239	8.53923205342237	0.0	2.592312238824542E-4
240-244	8.820446085090058	0.0	2.592312238824542E-4
245-249	9.068789597569447	0.0	2.592312238824542E-4
250-254	9.301734775350223	0.0	2.592312238824542E-4
255-259	9.52586609151899	0.0	5.184624477649084E-4
260-264	9.690322379950022	0.0	6.740011820943809E-4
265-269	9.811849977706114	0.0	7.776936716473626E-4
270-274	9.897551820321654	0.0	8.295399164238535E-4
275-279	9.9583156191997	0.0	0.0011406173850827986
280-284	10.007569551737367	0.0	0.001296156119412271
285-289	10.048113315152582	0.0	0.001296156119412271
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7230	0.0	287.04358	2
CCGGGTT	7235	0.0	285.82587	1
GGGGGGA	5850	0.0	272.3077	1
TTATTAG	7710	0.0	268.79053	6
GTTATTA	7710	0.0	268.79053	5
GGGTTAT	7855	0.0	264.2043	3
TATTAGT	7880	0.0	263.17892	7
TTAGTAG	8310	0.0	252.04573	9
GGGAGCA	6430	0.0	247.51555	4
GGTTATT	8435	0.0	246.2122	4
GGGGAGC	6505	0.0	245.56879	3
CAGAAGC	6665	0.0	242.99326	9
GAGCAGA	6565	0.0	242.42574	6
GGGGGAG	6645	0.0	241.06096	2
GGAGCAG	6775	0.0	234.2583	5
GCAGAAG	7045	0.0	229.67706	8
ATTAGTA	9600	0.0	218.63802	8
AGCAGAA	7420	0.0	213.89488	7
CGGGTAA	135	0.0	131.11111	2
GTAATTA	185	0.0	95.675674	5
>>END_MODULE