##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30455_flu.3520000003bb32.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401532
Sequences flagged as poor quality	0
Sequence length	301
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16332695775181	34.0	34.0	34.0	34.0	34.0
2	33.35722433081298	34.0	34.0	34.0	34.0	34.0
3	33.389015072273196	34.0	34.0	34.0	34.0	34.0
4	33.378375820607076	34.0	34.0	34.0	34.0	34.0
5	33.33784604962992	34.0	34.0	34.0	34.0	34.0
6	36.78565842821992	38.0	38.0	38.0	35.0	38.0
7	36.027193349471524	38.0	38.0	38.0	34.0	38.0
8	36.70712919518245	38.0	38.0	38.0	34.0	38.0
9	36.80689708416764	38.0	38.0	38.0	36.0	38.0
10-14	36.794695316936135	38.0	38.0	38.0	36.0	38.0
15-19	36.69708616000717	38.0	38.0	38.0	35.6	38.0
20-24	36.772728948128666	38.0	38.0	38.0	35.8	38.0
25-29	36.654543598019586	38.0	38.0	38.0	35.0	38.0
30-34	36.29043413725432	38.0	37.4	38.0	33.0	38.0
35-39	36.57774872239323	38.0	38.0	38.0	34.4	38.0
40-44	36.063130211290755	38.0	37.0	38.0	32.6	38.0
45-49	36.36731767331122	38.0	37.0	38.0	34.0	38.0
50-54	36.3199889423508	38.0	37.0	38.0	34.0	38.0
55-59	35.93581781775798	38.0	37.0	38.0	32.4	38.0
60-64	36.10993345486786	38.0	37.0	38.0	33.0	38.0
65-69	35.93347877628682	38.0	37.0	38.0	31.8	38.0
70-74	35.80474233684987	38.0	37.0	38.0	31.4	38.0
75-79	35.86645796599025	38.0	37.0	38.0	31.8	38.0
80-84	35.01859976290807	38.0	36.2	38.0	27.4	38.0
85-89	35.25553131506331	38.0	36.4	38.0	28.0	38.0
90-94	35.20238586214797	38.0	36.4	38.0	27.8	38.0
95-99	34.28257075401213	38.0	34.8	38.0	23.4	38.0
100-104	34.53195262145981	38.0	35.2	38.0	24.0	38.0
105-109	34.24685604136158	37.8	34.8	38.0	23.8	38.0
110-114	33.7947077692438	37.0	34.0	38.0	23.0	38.0
115-119	33.76565055836148	37.0	34.0	38.0	23.0	38.0
120-124	33.17396222467948	37.0	32.8	38.0	21.4	38.0
125-129	32.636144566311025	37.0	30.8	38.0	19.6	38.0
130-134	32.46695306974288	36.8	30.2	38.0	19.4	38.0
135-139	31.69474861281293	36.0	28.0	38.0	15.8	38.0
140-144	30.36902463564548	35.2	25.2	37.6	13.2	38.0
145-149	29.963987428150187	35.0	23.8	37.6	10.6	38.0
150-154	28.962972814121912	34.0	22.6	37.0	10.0	38.0
155-159	28.838511002859054	33.2	22.4	37.0	10.0	37.0
160-164	27.93124931512308	31.8	21.0	37.0	10.0	37.2
165-169	26.589552015779567	29.4	19.6	35.8	9.0	37.0
170-174	25.92978841038821	28.4	18.4	35.4	9.0	37.0
175-179	25.132602631919745	26.8	17.2	35.0	8.8	37.0
180-184	24.12291423846667	25.2	15.4	35.0	8.0	36.6
185-189	23.373745554526163	24.4	14.6	34.2	8.0	36.0
190-194	22.433255630933523	23.2	12.8	32.4	8.0	36.0
195-199	21.630623711186153	22.2	10.8	31.4	8.0	35.8
200-204	20.869457477859797	20.6	9.4	29.0	8.0	35.0
205-209	19.916264706175347	19.6	9.0	28.2	8.0	34.8
210-214	18.72493599513862	18.0	9.0	26.2	7.8	34.0
215-219	18.278283175438077	17.2	9.0	25.6	7.4	33.0
220-224	17.642662602233447	16.0	8.8	25.0	7.4	32.0
225-229	16.99753792972914	15.2	8.0	23.8	7.0	31.2
230-234	16.23117559746172	14.2	8.0	22.6	7.0	29.6
235-239	15.362763615353197	13.0	8.0	20.8	7.0	28.0
240-244	14.700126515445842	12.2	8.0	19.8	7.0	26.8
245-249	14.058807766255242	11.8	8.0	19.0	7.0	25.4
250-254	13.445895719394718	11.0	7.4	17.8	7.0	24.6
255-259	12.92828616399191	10.0	7.6	16.6	7.0	23.4
260-264	12.447630076805833	9.4	7.0	15.8	7.0	22.2
265-269	12.055060119741391	9.0	7.0	14.6	7.0	21.2
270-274	11.69841158363468	8.2	7.0	13.8	7.0	20.6
275-279	11.384687646314614	8.0	7.0	13.0	7.0	19.6
280-284	11.195999322594464	8.0	7.0	13.0	7.0	19.2
285-289	10.914190649811223	8.0	7.0	13.0	7.0	19.0
290-294	10.770875546656304	8.0	7.0	13.0	7.0	18.8
295-299	10.521725292131139	8.0	7.0	12.8	7.0	18.0
300-301	10.291305549744479	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.22762447654261564
1101	2	0.10822319204191899
1101	3	0.0471614045235782
1101	4	0.18335840225864075
1101	5	0.11557876620920382
1101	6	0.14093993778330116
1101	7	0.8950654685606381
1101	8	0.2802378165806516
1101	9	0.05990112716997231
1101	10-14	0.03548599568215849
1101	15-19	0.1653012815240018
1101	20-24	0.02521168626939385
1101	25-29	-0.006105290899974136
1101	30-34	0.3110459942468182
1101	35-39	0.1277979594409686
1101	40-44	0.4216568474756315
1101	45-49	0.06970581399902898
1101	50-54	0.09833529474732217
1101	55-59	0.277796982746743
1101	60-64	0.12519365456459752
1101	65-69	-0.010144150871141733
1101	70-74	0.035322278423208786
1101	75-79	0.17934622269856249
1101	80-84	0.6404963362778133
1101	85-89	0.17426696570017697
1101	90-94	0.09447276387645331
1101	95-99	0.7821552219859313
1101	100-104	0.20590913489827045
1101	105-109	0.36739525774173387
1101	110-114	0.41451671588855277
1101	115-119	0.4735654584737148
1101	120-124	0.7056064023151194
1101	125-129	0.4616029372815973
1101	130-134	0.5828245308617994
1101	135-139	0.2351087681985753
1101	140-144	0.6235153510953246
1101	145-149	1.0562523541375057
1101	150-154	0.8461527673262275
1101	155-159	0.524252767269676
1101	160-164	0.7549414745643972
1101	165-169	0.33082307254422716
1101	170-174	0.265481326494303
1101	175-179	0.31455374414216664
1101	180-184	-0.001665459203472608
1101	185-189	0.3300209310104911
1101	190-194	0.7504728686065967
1101	195-199	0.5365451718061713
1101	200-204	0.5706655435475909
1101	205-209	0.24421500663993356
1101	210-214	0.4603381764043135
1101	215-219	0.6003905436535426
1101	220-224	0.3150549169664707
1101	225-229	0.393198467014507
1101	230-234	0.1659473495018915
1101	235-239	0.18280540282393432
1101	240-244	0.0369031665498909
1101	245-249	0.14460429496220328
1101	250-254	0.13946912481782547
1101	255-259	0.0584373291829241
1101	260-264	0.07481786021254777
1101	265-269	0.18923986112896607
1101	270-274	0.19166609515911226
1101	275-279	-0.04963439639889877
1101	280-284	-0.06348425498930155
1101	285-289	-0.06754846217379473
1101	290-294	-0.10621294563118511
1101	295-299	-0.17355029250773057
1101	300-301	-0.3937583120637207
1102	1	-0.003982032904758626
1102	2	0.09003567358644915
1102	3	0.07565026197995195
1102	4	0.07684895488360155
1102	5	0.007201899245309562
1102	6	-0.0881466033072229
1102	7	0.9252877726046123
1102	8	0.10266981844716128
1102	9	0.004261319507804728
1102	10-14	0.0669955341952928
1102	15-19	0.2097236300433707
1102	20-24	0.11844960409199956
1102	25-29	0.17620553375397208
1102	30-34	0.5055097194618128
1102	35-39	0.24429286117587878
1102	40-44	0.5559750798340346
1102	45-49	0.12109712817392904
1102	50-54	0.20933748570714528
1102	55-59	0.4155183116130985
1102	60-64	0.3318933982842722
1102	65-69	0.40641618801676316
1102	70-74	0.2012096536095882
1102	75-79	0.31394844136436717
1102	80-84	0.7221892446402904
1102	85-89	0.17855738271165222
1102	90-94	0.344124798625586
1102	95-99	0.8685977010879711
1102	100-104	0.7492917800174581
1102	105-109	0.9259370846595516
1102	110-114	0.8929339388013986
1102	115-119	0.8469429269490618
1102	120-124	1.1838641335598084
1102	125-129	0.8819721194812331
1102	130-134	0.9642372807168798
1102	135-139	1.0871664841320658
1102	140-144	1.8399129173436073
1102	145-149	1.792919579784492
1102	150-154	1.800995481199994
1102	155-159	1.1434101773437142
1102	160-164	1.2215792808339714
1102	165-169	1.3313646151111875
1102	170-174	1.679633250054959
1102	175-179	1.0966760952134074
1102	180-184	1.1596484240449776
1102	185-189	1.1909994260653392
1102	190-194	1.2906481818482334
1102	195-199	1.4910162245240919
1102	200-204	1.399833379819821
1102	205-209	1.011823980461422
1102	210-214	1.6250755710942428
1102	215-219	1.486644549172862
1102	220-224	1.0339028220661923
1102	225-229	1.136274341302265
1102	230-234	0.8753057737606689
1102	235-239	0.7327277531660634
1102	240-244	0.46037450726419316
1102	245-249	0.1608523947329008
1102	250-254	0.6677802629868257
1102	255-259	0.4499824480087753
1102	260-264	0.4500807936197635
1102	265-269	0.21196642132203358
1102	270-274	0.2190106226809938
1102	275-279	0.11303817358604462
1102	280-284	0.18661636146878102
1102	285-289	0.10866722888993507
1102	290-294	-0.07211417380816876
1102	295-299	0.12794984055388703
1102	300-301	-0.13774638755522872
1103	1	-0.07765785399629976
1103	2	0.07236936349399059
1103	3	0.17694469695105397
1103	4	0.11458403790184235
1103	5	0.07643466453122016
1103	6	0.3060108753954154
1103	7	1.116048793748405
1103	8	0.5780556070233729
1103	9	0.29717290675727526
1103	10-14	0.07954182705921653
1103	15-19	0.21120023559815593
1103	20-24	0.2966378540893544
1103	25-29	0.05838388440410114
1103	30-34	0.5731710869415423
1103	35-39	0.16567989952702078
1103	40-44	0.5597868933845618
1103	45-49	0.2070177427525124
1103	50-54	0.27369136265726723
1103	55-59	0.4682453981900281
1103	60-64	0.45130904024140506
1103	65-69	0.38493788785188343
1103	70-74	0.2514018859406377
1103	75-79	0.24680234126234524
1103	80-84	0.8947821039770503
1103	85-89	0.553020667925118
1103	90-94	0.634362785428813
1103	95-99	1.3051382605211188
1103	100-104	0.7381396569887642
1103	105-109	0.9588451767757391
1103	110-114	0.7587679517439909
1103	115-119	0.8089172889107061
1103	120-124	1.127717465057323
1103	125-129	1.1451028763011522
1103	130-134	1.3151025909893903
1103	135-139	1.3538130273040245
1103	140-144	1.5887385172532298
1103	145-149	1.64153176473706
1103	150-154	2.1162047831971655
1103	155-159	0.7103068520244769
1103	160-164	0.7812389113383631
1103	165-169	1.6900689091825214
1103	170-174	1.893685804150092
1103	175-179	1.6919551939632775
1103	180-184	1.4173663138317778
1103	185-189	1.0996978949145237
1103	190-194	1.0023856514423386
1103	195-199	1.4423865639050675
1103	200-204	1.1912035779832912
1103	205-209	0.901208483958321
1103	210-214	1.4402336817452195
1103	215-219	1.3352645010883002
1103	220-224	1.2344763416654168
1103	225-229	0.69644182526525
1103	230-234	1.2280882882089834
1103	235-239	0.7637011654399792
1103	240-244	0.7285077177382959
1103	245-249	0.6792336184532068
1103	250-254	0.6555356460216597
1103	255-259	0.6933063903779111
1103	260-264	0.4131036955689176
1103	265-269	0.23705618269975126
1103	270-274	0.17964288876301993
1103	275-279	0.04378069523645145
1103	280-284	0.3844883901641776
1103	285-289	0.17684262809417284
1103	290-294	0.08487803451589748
1103	295-299	0.0851164524987702
1103	300-301	-0.02606672887794126
2116	1	0.02392405958794086
2116	2	0.008040802245041334
2116	3	0.04034475309322261
2116	4	0.02433679642573594
2116	5	0.1999819859019567
2116	6	0.08657618385344534
2116	7	0.9897841913861143
2116	8	0.24647563682190565
2116	9	0.3959157540289837
2116	10-14	0.2871177377499876
2116	15-19	0.2642195182739826
2116	20-24	0.1443808093267691
2116	25-29	0.13821632162203912
2116	30-34	0.4216637560698189
2116	35-39	-0.038911666191708605
2116	40-44	0.40372289131162375
2116	45-49	0.20711534375443108
2116	50-54	0.2823536675092484
2116	55-59	0.43472213157949824
2116	60-64	0.2379936724022329
2116	65-69	0.4653170634257293
2116	70-74	0.4553979991750694
2116	75-79	0.4888293326898605
2116	80-84	0.88641985742656
2116	85-89	0.7046682678473815
2116	90-94	0.7791121538294092
2116	95-99	1.326113838677493
2116	100-104	0.7296955292437346
2116	105-109	0.7363618790710902
2116	110-114	0.7621794954377066
2116	115-119	0.8143798356058767
2116	120-124	1.0880423986584162
2116	125-129	1.184714890725374
2116	130-134	0.921003219349835
2116	135-139	1.4435088663055602
2116	140-144	1.8345755754035586
2116	145-149	1.9334580889364972
2116	150-154	2.06914244379616
2116	155-159	1.926621248172264
2116	160-164	1.8193440699399979
2116	165-169	1.8180200223089962
2116	170-174	1.4824126293104243
2116	175-179	1.9615787391253328
2116	180-184	1.900088604432284
2116	185-189	1.281729464088155
2116	190-194	1.3668099998692789
2116	195-199	0.8673062590098972
2116	200-204	0.8289917492600871
2116	205-209	0.5834386236659483
2116	210-214	1.3897148849150902
2116	215-219	1.4535641707132285
2116	220-224	1.142703069656612
2116	225-229	0.4096848992649491
2116	230-234	1.096804403170875
2116	235-239	0.820364679507918
2116	240-244	0.9309950749536444
2116	245-249	0.7245294660919956
2116	250-254	0.7920030943600675
2116	255-259	1.3331220713176446
2116	260-264	0.6083632235428347
2116	265-269	0.7732054344973811
2116	270-274	0.5710486454946828
2116	275-279	0.2084957737275257
2116	280-284	0.1693619623176641
2116	285-289	0.05012854288763613
2116	290-294	0.12571481195538325
2116	295-299	0.03803632034874127
2116	300-301	-0.1768192498772727
1117	1	0.07949853870958634
1117	2	0.022576982758572228
1117	3	0.09848515250534717
1117	4	-0.023434449359690745
1117	5	0.04707284783676613
1117	6	0.06814270931380406
1117	7	0.8856889203564648
1117	8	0.2725498133586086
1117	9	0.07233487739718925
1117	10-14	0.110649682074353
1117	15-19	0.1311796635147502
1117	20-24	0.1158403061712363
1117	25-29	0.143058545189092
1117	30-34	0.3635658914549751
1117	35-39	0.10743829490451162
1117	40-44	0.3635188626092756
1117	45-49	0.11403675812290004
1117	50-54	0.16677313668881055
1117	55-59	0.2576331716071607
1117	60-64	0.2899177668087276
1117	65-69	0.3521347343862118
1117	70-74	0.2763534759624875
1117	75-79	0.475324188745887
1117	80-84	0.73112531260076
1117	85-89	0.375149464354692
1117	90-94	0.430462028472796
1117	95-99	0.8963783843678215
1117	100-104	0.4922858292350938
1117	105-109	0.47856193094278865
1117	110-114	0.7101933278921067
1117	115-119	0.5727984402570385
1117	120-124	0.974255245550431
1117	125-129	0.7943514429861196
1117	130-134	0.6816189364761414
1117	135-139	1.0114445453926102
1117	140-144	1.3357063785504408
1117	145-149	1.7473035112586182
1117	150-154	2.007379497483374
1117	155-159	1.4786795173864071
1117	160-164	1.8183035235580398
1117	165-169	1.6169032245221828
1117	170-174	1.3362886559378993
1117	175-179	1.5384446507705256
1117	180-184	1.4327596513761733
1117	185-189	1.2017815086978167
1117	190-194	-0.5418726317551688
1117	195-199	1.081507694127044
1117	200-204	1.6486488772967434
1117	205-209	1.6660771643420134
1117	210-214	1.5784735950387194
1117	215-219	1.5123891037122768
1117	220-224	1.1754353252111969
1117	225-229	1.355241223290193
1117	230-234	1.1333251950788892
1117	235-239	0.7144972981644386
1117	240-244	0.479843177313807
1117	245-249	0.6226783378824354
1117	250-254	0.53234907862565
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2108	240-244	-0.7525368417966529
2108	245-249	-0.9393295155389652
2108	250-254	-0.7480630516323394
2108	255-259	-0.48116808095776165
2108	260-264	-0.30005581742754117
2108	265-269	-0.14710662100242367
2108	270-274	-0.46661739578111394
2108	275-279	-0.25605241772934484
2108	280-284	-0.6043812201946626
2108	285-289	-0.89501199553027
2108	290-294	-0.6176102894888338
2108	295-299	-0.5171924418136573
2108	300-301	2.481368280119673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	96.0
11	572.0
12	836.0
13	956.0
14	1422.0
15	2176.0
16	3186.0
17	4803.0
18	5919.0
19	6926.0
20	9118.0
21	11982.0
22	15545.0
23	20835.0
24	28262.0
25	37315.0
26	46659.0
27	53673.0
28	55281.0
29	48118.0
30	31252.0
31	13163.0
32	3092.0
33	329.0
34	11.0
35	0.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.752986063377264	18.132801370750027	12.89262126057201	32.2215913053007
2	52.05911359493142	21.255591086140083	13.330942490262295	13.354352828666208
3	51.773457657173026	20.15107139655121	15.373868085233555	12.701602861042208
4	51.987637348953506	18.952412261040216	15.586055407788171	13.473894982218104
5	35.2935756054312	19.230845860354844	32.417092535588694	13.058485998625265
6	34.26800354641722	18.990516322484883	32.51023579689788	14.231244334200014
7	16.046292698962972	52.38685833258619	17.34581552653338	14.221033441917456
8	33.29871591803393	17.714154787165157	34.0986521622187	14.888477132582208
9	14.620005379396911	16.722203958837653	34.93220963708995	33.72558102467549
10-14	27.56970801828995	36.95172489365729	20.547552872498333	14.931014215554427
15-19	23.117958220017336	34.95522150164869	18.724584840062562	23.20223543827142
20-24	25.445344331211462	29.049888925415658	25.249743482462172	20.25502326091071
25-29	21.5872209437853	23.99280754709463	36.066664674297435	18.35330683482263
30-34	21.264307701503242	26.27915085223594	29.27069324487214	23.18584820138868
35-39	20.621071296932747	28.334977037944668	27.88769014673799	23.15626151838459
40-44	23.678959584790256	26.270384427642128	27.65707340884413	22.39358257872349
45-49	23.200541924429434	27.162666985445743	25.167757488817827	24.469033601306993
50-54	22.740304633254635	27.601336879750555	27.001932598149086	22.65642588884572
55-59	23.04702987557654	25.650608170706196	26.76663379257444	24.53572816114282
60-64	23.110586453881634	25.468555432692785	27.29207136666567	24.128786746759907
65-69	19.681171114630963	30.186336331849017	26.107358815735733	24.025133737784284
70-74	21.250410926152835	25.71854796130819	27.99084506340715	25.040196049131826
75-79	21.327067332117988	24.457577478258273	27.075301594891567	27.140053594732176
80-84	27.27573393901358	23.79745574449857	25.885608120896965	23.041202195590888
85-89	22.390046123347577	28.45631232379985	24.06622635306775	25.087415199784825
90-94	22.486327366187503	28.055547254017117	27.477311895440465	21.980813484354922
95-99	22.05468057340386	28.135092595359772	25.6682406383551	24.141986192881266
100-104	22.58310670133389	28.783558969148164	23.43379855154757	25.19953577797037
105-109	25.38811352519849	25.670183198350315	23.394548877798034	25.54715439865316
110-114	20.750326250460734	27.72705537790263	28.272566071944453	23.25005229969218
115-119	25.984379825269222	26.045545560503275	23.216630305928295	24.753444308299212
120-124	19.885986671049878	25.36046940218961	28.596575117300738	26.15696880945977
125-129	22.845601346841597	22.990944681868445	30.58017791857187	23.583276052718087
130-134	26.30928543677714	25.94164350537442	24.603269477899644	23.145801579948795
135-139	25.5037207495293	26.22749868005539	22.74732773477581	25.5214528356395
140-144	22.00193259814909	25.206558879491546	26.44207684568104	26.34943167667832
145-149	21.31247322753853	25.443501389677536	28.252443142763216	24.991582240020723
150-154	23.072681629359554	23.948029048743315	25.834852514868057	27.144436807029077
155-159	23.52206050825339	24.233585368040405	28.46642359762111	23.777930526085093
160-164	26.032146877459333	26.590956636083803	24.382116493828637	22.994779992628235
165-169	22.955082035802874	23.965163424085752	26.993166173555284	26.08658836655609
170-174	23.994451251705968	27.132133927059364	25.2662303378062	23.60718448342847
175-179	23.156859229152346	26.91650976759013	25.25442555014295	24.67220545311457
180-184	21.642808045186936	27.446928264746024	26.402080033471805	24.50818365659524
185-189	22.51242740304633	25.04956018449339	26.763495811043704	25.674516601416574
190-194	21.40711076576711	24.96488449239413	25.80715858262853	27.820846159210227
195-199	21.44825319028122	27.890330035962265	27.526722652241915	23.1346941215146
200-204	24.173366008188637	25.92341332695775	23.818923522907266	26.084297141946344
205-209	23.19974497673909	25.611906398493765	26.934391281392266	24.253957343374875
210-214	25.222049550222646	24.94097606168375	25.3385533407051	24.4984210473885
215-219	24.745375212934462	24.21655061115926	26.185708735542875	24.852365440363407
220-224	20.613898767719636	25.608021278503333	27.245649163703018	26.53243079007402
225-229	23.78322215077361	26.053926488512243	23.84100088710284	26.321850473611306
230-234	23.380801530139568	24.79289321897134	26.177589830947472	25.648715419941624
235-239	23.387376348584922	25.089407569010692	23.373280336311925	28.149935746092464
240-244	24.22691093113376	27.478108843130812	22.57673111981112	25.71824910592431
245-249	23.651315461781376	26.523813793172152	21.8416963031589	27.98317444188757
250-254	23.603747646513852	24.880109181833575	23.273412828865446	28.242730342787127
255-259	23.82993136288017	25.106392516661185	24.766544135959276	26.297131984499366
260-264	23.924419473416812	26.28179074146021	23.262903081199006	26.530886703923972
265-269	22.99627426954763	26.38499546736001	23.66556090174631	26.953169361346045
270-274	21.729127441897532	27.71385593178128	22.866720460635765	27.690296165685425
275-279	22.578773298267635	27.47437315083231	20.77757189962444	29.169281651275615
280-284	20.623163284619906	27.618321827401054	23.84507336899674	27.913441518982296
285-289	21.85748582927388	27.063048524152496	23.73539344311288	27.344072203460744
290-294	21.452935257962004	29.10896267296255	21.914517398364264	27.523584670711177
295-299	20.95603837303129	30.01753284918761	20.24884691631053	28.77758186147057
300-301	17.01645198888258	29.883795064901424	26.051597382026838	27.04815556418916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.25
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.25
21	0.5
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	0.5
28	0.75
29	0.5
30	0.5
31	2.25
32	4.5
33	12.25
34	26.5
35	81.75
36	293.5
37	837.25
38	1876.5
39	3141.5
40	4500.25
41	6019.75
42	8309.5
43	10694.75
44	12513.75
45	12162.75
46	10065.5
47	8116.75
48	6868.75
49	6033.75
50	5284.0
51	4315.75
52	2911.25
53	1864.75
54	1371.0
55	1280.0
56	1379.5
57	1577.25
58	1927.25
59	2478.25
60	3170.0
61	3734.5
62	3663.0
63	2926.0
64	1990.5
65	1123.75
66	562.25
67	272.25
68	136.0
69	79.0
70	51.25
71	33.25
72	25.0
73	17.0
74	14.5
75	13.25
76	8.5
77	5.5
78	7.0
79	5.5
80	3.0
81	2.5
82	2.5
83	1.5
84	1.25
85	1.75
86	1.25
87	0.5
88	1.5
89	0.75
90	1.0
91	0.25
92	0.5
93	0.75
94	2.0
95	1.0
96	1.25
97	1.0
98	0.75
99	0.25
100	1.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	4.9809230646623434E-5
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	401532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.447342842441097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.67900531172847	8.600926841763686
2	7.612252129272005	2.351781368879338
3	5.202782924692132	2.4110751471755325
4	4.011527964273029	2.4786979114466106
5	3.8419745474496954	2.9674149013193962
6	3.326594690722734	3.0832229211263025
7	2.9540892461953496	3.1942980561203558
8	2.5194030552429294	3.1134466202104876
9	2.058357958466473	2.861654496920886
>10	12.201339295557036	32.742669777646334
>50	0.4179199250351583	4.272673525949014
>100	0.1195032969110905	3.317768097094389
>500	0.015068087578494522	1.7043344650680456
>1k	0.026787711250281735	9.608411843013055
>5k	0.01171962367199826	13.239756338268933
>10k+	0.0016742319531426084	4.051867687997565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	15667	3.9018060827032466	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9467	2.35771993265792	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	9372	2.334060548100774	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7749	1.9298586414034247	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	7073	1.7615034418178375	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6826	1.6999890419692576	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	5510	1.3722443043144756	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	5196	1.2940438121992768	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4208	1.047986212804957	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4167	1.0377753205223992	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3538	0.8811252901387685	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	3038	0.7566022135222099	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2893	0.720490521303408	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2673	0.6657003675921221	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2650	0.6599723060677605	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2250	0.5603538447745136	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2161	0.5381887371367662	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1989	0.49535279878067	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1488	0.37058067601087835	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1377	0.3429365530020023	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1310	0.32625046073538344	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT	1164	0.28988972236334837	Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1159	0.2886444915971828	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGTTTAGCCAAATA	1087	0.27071316856439837	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	979	0.24381618401522173	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	874	0.2176663379257444	No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT	820	0.20421784565115608	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	700	0.17433230726318202	No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG	673	0.16760806112588786	No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG	650	0.16187999960152616	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	647	0.1611328611418268	No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG	645	0.16063476883536057	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGCCATGGC	602	0.14992578424633654	No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC	488	0.12153452277776117	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	459	0.11431218433400078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	2.4904615323311717E-4	0.0	0.0
4	2.4904615323311717E-4	0.0	0.0
5	2.4904615323311717E-4	0.0	0.0
6	2.4904615323311717E-4	0.0	0.0
7	2.4904615323311717E-4	0.0	0.0
8	7.471384596993514E-4	0.0	0.0
9	9.961846129324687E-4	0.0	0.0
10-14	0.0021417969178048075	0.0	0.0
15-19	0.0024904615323311716	0.0	0.0
20-24	0.0025900799936244183	0.0	0.0
25-29	0.0029387446081507824	0.0	0.0
30-34	0.007023101521173904	0.0	0.0
35-39	0.007471384596993515	0.0	1.494276919398703E-4
40-44	0.00911508920833209	0.0	2.4904615323311717E-4
45-49	0.014295249195580926	0.0	2.4904615323311717E-4
50-54	0.026000418397537434	0.0	2.4904615323311717E-4
55-59	0.03033382146379367	0.0	2.4904615323311717E-4
60-64	0.038004442983373674	0.0	2.4904615323311717E-4
65-69	0.04622296604006655	0.0	2.4904615323311717E-4
70-74	0.0652500921470767	0.0	2.4904615323311717E-4
75-79	0.08636920594124503	0.0	2.4904615323311717E-4
80-84	0.10958030742257155	0.0	2.4904615323311717E-4
85-89	0.14325134733968897	0.0	2.4904615323311717E-4
90-94	0.1848918641602662	0.0	2.4904615323311717E-4
95-99	0.22917227020511444	0.0	2.4904615323311717E-4
100-104	0.28944143928752875	0.0	2.4904615323311717E-4
105-109	0.3570823745056434	0.0	2.4904615323311717E-4
110-114	0.4413595927597303	0.0	2.4904615323311717E-4
115-119	0.5355986571431417	0.0	2.4904615323311717E-4
120-124	0.6443322076447207	0.0	2.4904615323311717E-4
125-129	0.7781696103921979	0.0	2.4904615323311717E-4
130-134	0.9419423607582958	0.0	2.4904615323311717E-4
135-139	1.123795861849118	0.0	2.4904615323311717E-4
140-144	1.3287110367293267	0.0	2.4904615323311717E-4
145-149	1.604106272974508	0.0	2.4904615323311717E-4
150-154	1.9149158722094377	0.0	2.4904615323311717E-4
155-159	2.265174382116494	0.0	2.4904615323311717E-4
160-164	2.6557285596166684	0.0	2.4904615323311717E-4
165-169	3.1124792046462053	0.0	2.4904615323311717E-4
170-174	3.6105217018817926	0.0	2.4904615323311717E-4
175-179	4.182082623551797	0.0	2.4904615323311717E-4
180-184	4.840759889622745	0.0	2.4904615323311717E-4
185-189	5.536246177141548	0.0	2.4904615323311717E-4
190-194	6.272376796867996	0.0	2.4904615323311717E-4
195-199	6.995407588934381	0.0	2.4904615323311717E-4
200-204	7.680135082633514	0.0	2.4904615323311717E-4
205-209	8.358088520964705	0.0	2.4904615323311717E-4
210-214	9.038781466981462	0.0	2.4904615323311717E-4
215-219	9.689389637687658	0.0	2.4904615323311717E-4
220-224	10.315392048454418	0.0	2.4904615323311717E-4
225-229	10.877190360917684	0.0	2.4904615323311717E-4
230-234	11.394658458105456	0.0	3.9847384517298747E-4
235-239	11.842841915463774	0.0	4.980923064662343E-4
240-244	12.227070320671826	0.0	4.980923064662343E-4
245-249	12.497135969237819	0.0	4.980923064662343E-4
250-254	12.74583345785641	0.0	4.980923064662343E-4
255-259	12.93241883585866	0.0	4.980923064662343E-4
260-264	13.088471155474531	0.0	4.980923064662343E-4
265-269	13.216480878236354	0.0	4.980923064662343E-4
270-274	13.320183696442623	0.0	4.980923064662343E-4
275-279	13.38722692089298	0.0	4.980923064662343E-4
280-284	13.429465148481317	0.0	4.980923064662343E-4
285-289	13.462538477630673	0.0	4.980923064662343E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6295	0.0	289.3765	1
GGGTTAT	6310	0.0	288.6886	2
GTTATTA	6315	0.0	287.05856	4
GGTTATT	6360	0.0	285.49136	3
TTATTAG	6385	0.0	282.29443	5
TTAGTAG	6515	0.0	279.37836	8
GGGGGGA	8040	0.0	274.26926	1
ATTAGTA	6590	0.0	274.1844	7
TATTAGT	6600	0.0	273.76892	6
TAGTAGA	6745	0.0	268.75836	9
GGGGAGC	8200	0.0	268.37805	3
GGAGCGA	5680	0.0	264.09772	5
GGGAGCG	5740	0.0	262.10803	4
CGAAAGC	5795	0.0	260.6385	9
GCGAAAG	5920	0.0	255.63344	8
AGCGAAA	5850	0.0	255.41452	7
GGGGGAG	8770	0.0	252.61688	2
GAGCGAA	5935	0.0	252.00505	6
GGGAGCA	2845	0.0	243.15465	4
GCAAAAG	3455	0.0	194.24747	8
>>END_MODULE