FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30455_flu.3520000003bb32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30455_flu.3520000003bb32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401532
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC156673.9018060827032466No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT94672.35771993265792No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG93722.334060548100774No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG77491.9298586414034247No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC70731.7615034418178375No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA68261.6999890419692576No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT55101.3722443043144756No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC51961.2940438121992768No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG42081.047986212804957No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA41671.0377753205223992No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT35380.8811252901387685No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA30380.7566022135222099No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT28930.720490521303408No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26730.6657003675921221No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA26500.6599723060677605No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA22500.5603538447745136No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG21610.5381887371367662No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA19890.49535279878067No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA14880.37058067601087835No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG13770.3429365530020023No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13100.32625046073538344No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT11640.28988972236334837Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11590.2886444915971828No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGTTTAGCCAAATA10870.27071316856439837No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9790.24381618401522173No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8740.2176663379257444No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT8200.20421784565115608No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7000.17433230726318202No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG6730.16760806112588786No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG6500.16187999960152616No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6470.1611328611418268No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG6450.16063476883536057No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGCCATGGC6020.14992578424633654No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC4880.12153452277776117No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4590.11431218433400078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA62950.0289.37651
GGGTTAT63100.0288.68862
GTTATTA63150.0287.058564
GGTTATT63600.0285.491363
TTATTAG63850.0282.294435
TTAGTAG65150.0279.378368
GGGGGGA80400.0274.269261
ATTAGTA65900.0274.18447
TATTAGT66000.0273.768926
TAGTAGA67450.0268.758369
GGGGAGC82000.0268.378053
GGAGCGA56800.0264.097725
GGGAGCG57400.0262.108034
CGAAAGC57950.0260.63859
GCGAAAG59200.0255.633448
AGCGAAA58500.0255.414527
GGGGGAG87700.0252.616882
GAGCGAA59350.0252.005056
GGGAGCA28450.0243.154654
GCAAAAG34550.0194.247478
Produced by FastQC (version 0.11.2)