##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n02_30429_flu.3520000003bb0b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 398481 Sequences flagged as poor quality 0 Sequence length 301 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.19346970119027 34.0 34.0 34.0 34.0 34.0 2 33.405120946795456 34.0 34.0 34.0 34.0 34.0 3 33.44030204702357 34.0 34.0 34.0 34.0 34.0 4 33.42424607446779 34.0 34.0 34.0 34.0 34.0 5 33.339526853225124 34.0 34.0 34.0 34.0 34.0 6 36.797739415430094 38.0 38.0 38.0 35.0 38.0 7 36.09708116572685 38.0 38.0 38.0 34.0 38.0 8 36.74028623698495 38.0 38.0 38.0 34.0 38.0 9 36.804474993788915 38.0 38.0 38.0 36.0 38.0 10-14 36.829705305899154 38.0 38.0 38.0 36.0 38.0 15-19 36.717551401447 38.0 38.0 38.0 35.6 38.0 20-24 36.7977268677804 38.0 38.0 38.0 35.8 38.0 25-29 36.688107086661596 38.0 38.0 38.0 35.2 38.0 30-34 36.34382467420028 38.0 37.4 38.0 33.0 38.0 35-39 36.61565444776538 38.0 38.0 38.0 34.8 38.0 40-44 36.14296591305482 38.0 37.2 38.0 33.0 38.0 45-49 36.40618097224209 38.0 37.2 38.0 34.0 38.0 50-54 36.34863644690712 38.0 37.0 38.0 34.0 38.0 55-59 35.96520135213473 38.0 37.0 38.0 32.8 38.0 60-64 36.13858276806171 38.0 37.0 38.0 33.0 38.0 65-69 35.996225666970325 38.0 37.0 38.0 32.4 38.0 70-74 35.86970269598802 38.0 37.0 38.0 31.6 38.0 75-79 35.92175586790839 38.0 37.0 38.0 32.0 38.0 80-84 35.16187722877628 38.0 36.4 38.0 27.8 38.0 85-89 35.34499612277624 38.0 37.0 38.0 28.4 38.0 90-94 35.30300415829111 38.0 36.6 38.0 28.2 38.0 95-99 34.39785334808937 38.0 34.8 38.0 23.6 38.0 100-104 34.67355432253984 38.0 35.4 38.0 24.6 38.0 105-109 34.410579676320836 37.8 34.8 38.0 23.8 38.0 110-114 33.8943041198953 37.0 34.0 38.0 23.0 38.0 115-119 33.905402767007715 37.0 34.0 38.0 23.2 38.0 120-124 33.40464413610687 37.0 32.8 38.0 21.8 38.0 125-129 32.94909217754423 37.0 31.0 38.0 20.8 38.0 130-134 32.60945289737779 36.8 30.4 38.0 20.2 38.0 135-139 31.85158087838567 36.0 28.2 38.0 17.0 38.0 140-144 30.727633187027738 35.4 25.8 37.6 13.6 38.0 145-149 30.33828413399886 35.0 24.6 37.6 10.8 38.0 150-154 29.287666413204143 34.0 22.8 37.0 10.0 38.0 155-159 29.163681580803097 33.8 23.2 37.0 10.0 37.0 160-164 28.390396530825814 32.6 21.4 37.0 10.0 37.2 165-169 27.181059071825256 30.4 20.2 36.0 9.2 37.0 170-174 26.548348352870022 29.0 20.0 35.4 9.0 37.0 175-179 25.766543448746614 28.0 18.4 35.2 9.0 37.0 180-184 24.82689663999036 26.0 16.6 35.0 8.6 36.6 185-189 24.083289792988875 25.0 15.2 35.0 8.0 36.2 190-194 23.225553539566505 23.8 14.6 33.4 8.0 36.0 195-199 22.42599471492995 23.2 12.8 32.2 8.0 36.0 200-204 21.64733977278716 22.4 11.0 31.0 8.0 35.4 205-209 20.708402659097924 20.8 9.4 29.2 8.0 35.0 210-214 19.600652979690373 19.2 9.0 27.4 8.0 34.4 215-219 19.09798961556511 18.4 9.0 26.0 8.0 34.0 220-224 18.54093771095736 17.8 9.0 25.8 8.0 33.2 225-229 17.81063187454358 16.6 8.8 25.2 7.4 31.8 230-234 16.948205309663447 15.4 9.0 23.8 7.0 30.8 235-239 15.963004007719315 14.0 8.0 22.2 7.0 28.8 240-244 15.32639950210926 13.0 8.0 21.0 7.0 28.0 245-249 14.718959247743305 12.6 8.0 20.2 7.0 26.6 250-254 14.026072510358086 11.6 8.0 18.8 7.0 25.4 255-259 13.50982405685591 11.2 8.0 18.0 7.0 24.4 260-264 12.98876634017682 10.2 7.8 16.8 7.0 23.2 265-269 12.53407264085364 9.4 7.2 15.8 7.0 22.4 270-274 12.119474705192971 9.0 7.4 14.6 7.0 21.0 275-279 11.734862138972748 8.6 7.0 14.0 7.0 20.8 280-284 11.553875843515751 8.4 7.0 13.4 7.0 20.2 285-289 11.253314963574173 8.0 7.0 13.0 7.0 19.0 290-294 10.976475666342939 8.0 7.0 13.0 7.0 19.0 295-299 10.705689355326854 8.0 7.0 13.0 7.0 18.4 300-301 10.403358002012643 8.0 7.0 12.5 7.0 16.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.14378067879054868 1101 2 -0.026661997146206318 1101 3 0.13879955504037866 1101 4 0.11847293799913672 1101 5 0.21897170974938263 1101 6 0.14799778908083994 1101 7 0.9842898874349046 1101 8 0.28967235565134786 1101 9 0.2197123054151291 1101 10-14 0.10562231431212865 1101 15-19 0.18045836029723006 1101 20-24 0.09090013066354885 1101 25-29 0.006353234053143808 1101 30-34 0.4840707659470098 1101 35-39 0.2598570232859956 1101 40-44 0.5442789764299363 1101 45-49 0.2614758854366599 1101 50-54 0.2556006188675397 1101 55-59 0.23274695779501542 1101 60-64 0.26112942505777426 1101 65-69 0.22909384342590755 1101 70-74 0.20816802862399442 1101 75-79 0.35874245749336353 1101 80-84 0.7780362832550907 1101 85-89 0.349797150934414 1101 90-94 0.38994057352115874 1101 95-99 0.886582039797922 1101 100-104 0.22496758847848497 1101 105-109 0.38849172670686016 1101 110-114 0.4074654556082109 1101 115-119 0.2549147408730974 1101 120-124 0.725610897762273 1101 125-129 0.5329034530003298 1101 130-134 0.8181861698923498 1101 135-139 0.603255465139938 1101 140-144 0.9640094729845821 1101 145-149 0.9940550585956878 1101 150-154 0.7910161230329926 1101 155-159 0.48311855475174426 1101 160-164 1.0498058947033755 1101 165-169 0.30486803561522535 1101 170-174 0.5278255423149645 1101 175-179 0.4744569043774014 1101 180-184 0.18543753389564088 1101 185-189 0.5481750411864148 1101 190-194 0.741743871241713 1101 195-199 0.5100195410337278 1101 200-204 0.6562530076749518 1101 205-209 0.2161240561202753 1101 210-214 0.6218594680437128 1101 215-219 0.8868407498103004 1101 220-224 0.5667470240115868 1101 225-229 0.5308632038353629 1101 230-234 0.1959059354427204 1101 235-239 -0.08146694050439862 1101 240-244 -0.01683206112113389 1101 245-249 0.20226543304275957 1101 250-254 0.09936232680744084 1101 255-259 0.0165747688127329 1101 260-264 -0.03389548127367625 1101 265-269 -0.017947029949636928 1101 270-274 0.12479817795852632 1101 275-279 0.06563596637120739 1101 280-284 -0.06703439395760036 1101 285-289 0.01250942771186736 1101 290-294 -0.09507311889217185 1101 295-299 0.05914987462550414 1101 300-301 -0.1689736175317922 1102 1 -0.18564306820401555 1102 2 -0.10447902079107507 1102 3 -0.02359108766733442 1102 4 -0.007561398767144567 1102 5 0.06917586785205998 1102 6 -0.33426350757567036 1102 7 0.8589544454780764 1102 8 0.09149664559810589 1102 9 0.07366073965197018 1102 10-14 0.0029007573104138373 1102 15-19 0.06323891467418719 1102 20-24 0.05209264174392558 1102 25-29 0.059937618483864696 1102 30-34 0.4381734897081486 1102 35-39 0.006968594816640916 1102 40-44 0.406895726761924 1102 45-49 0.10578489796533574 1102 50-54 0.06557672235907575 1102 55-59 0.20112072784117885 1102 60-64 0.18865412515750535 1102 65-69 0.0450119303827492 1102 70-74 -0.036669058169380264 1102 75-79 0.2174538460807156 1102 80-84 0.7798036330202578 1102 85-89 -0.016978253111275876 1102 90-94 0.2021571813190235 1102 95-99 0.863889540656622 1102 100-104 0.4984672606132392 1102 105-109 0.6509020229611124 1102 110-114 0.6460034888943369 1102 115-119 0.6660915474840365 1102 120-124 0.8234607630344541 1102 125-129 0.7349169312923394 1102 130-134 0.7195321623137971 1102 135-139 0.8494732594977208 1102 140-144 1.4641259248398164 1102 145-149 1.5219493188417061 1102 150-154 1.3766822588702254 1102 155-159 0.7651704584824301 1102 160-164 1.1371673785277707 1102 165-169 1.382160658555506 1102 170-174 1.322137952886738 1102 175-179 1.1575967329323547 1102 180-184 1.1916767083732154 1102 185-189 1.3188866648816138 1102 190-194 1.0274017415687133 1102 195-199 1.289535402547859 1102 200-204 1.367024620606621 1102 205-209 1.005589571493207 1102 210-214 1.7426505324761195 1102 215-219 1.4325253269192402 1102 220-224 1.1508030384560293 1102 225-229 1.2689947576961842 1102 230-234 1.2515194107958827 1102 235-239 0.9693709067159073 1102 240-244 0.7896317515566906 1102 245-249 0.7340395237907167 1102 250-254 0.7762398374561723 1102 255-259 0.8810885455815072 1102 260-264 0.6790060618052429 1102 265-269 0.43257066650776643 1102 270-274 0.24054177081051442 1102 275-279 0.3205257503842418 1102 280-284 0.36909047769739445 1102 285-289 0.2723465787925363 1102 290-294 0.08752761471778037 1102 295-299 0.011780051819675563 1102 300-301 -0.13848444626858303 1103 1 -0.1048413529331853 1103 2 0.11077913905941728 1103 3 0.09295388748731881 1103 4 0.1519106194286337 1103 5 0.05931016312781878 1103 6 0.17694587426042574 1103 7 1.1231717082882753 1103 8 0.4960629747156915 1103 9 0.37145725405937924 1103 10-14 0.09796464535422444 1103 15-19 0.22544355923356107 1103 20-24 0.28664796067708664 1103 25-29 0.062307705551233994 1103 30-34 0.5794758061930025 1103 35-39 0.14667117496643556 1103 40-44 0.4503330773638723 1103 45-49 0.15839221104572232 1103 50-54 0.17701825972851282 1103 55-59 0.29981978762936734 1103 60-64 0.31229487442952575 1103 65-69 0.38149946424631054 1103 70-74 0.33726179497504916 1103 75-79 0.42358043422737524 1103 80-84 0.8863481735623608 1103 85-89 0.5056150799323191 1103 90-94 0.44501468635328933 1103 95-99 1.186438688091684 1103 100-104 0.7991311312725529 1103 105-109 0.8613276535114736 1103 110-114 0.7044889366176932 1103 115-119 0.8251622734318502 1103 120-124 1.3115197834177863 1103 125-129 1.0555153927639438 1103 130-134 1.2027079486003203 1103 135-139 1.2834835018666482 1103 140-144 1.76622981172466 1103 145-149 1.8777944953668424 1103 150-154 2.0395275947532845 1103 155-159 0.9804572303253316 1103 160-164 1.1073573042090281 1103 165-169 2.010315221262857 1103 170-174 2.048173779447353 1103 175-179 1.927764565809344 1103 180-184 1.6391675452921035 1103 185-189 1.565350367490737 1103 190-194 1.5740838369806553 1103 195-199 1.8734797286811116 1103 200-204 1.6368994521942604 1103 205-209 1.131847050450716 1103 210-214 1.957597944358426 1103 215-219 1.8933591310828284 1103 220-224 1.7147859726701746 1103 225-229 1.0988819757412998 1103 230-234 1.6717687335246723 1103 235-239 1.1380353423715217 1103 240-244 0.8193400541049698 1103 245-249 1.3145413564069361 1103 250-254 0.8253890834034099 1103 255-259 0.7597497940784717 1103 260-264 0.7663200838545858 1103 265-269 0.609226540296282 1103 270-274 0.48300667584767254 1103 275-279 0.518367174754415 1103 280-284 0.44704077250956864 1103 285-289 0.5215606544440927 1103 290-294 0.28323400497087015 1103 295-299 0.2516608231120685 1103 300-301 -0.22029935203009465 2116 1 0.24445565009825287 2116 2 0.19907840687348255 2116 3 0.16433146613928074 2116 4 0.16690161656600822 2116 5 0.24250786739785468 2116 6 0.3407645650472588 2116 7 1.1492668221252558 2116 8 0.35746109114802493 2116 9 0.4519625397244269 2116 10-14 0.22436536417654196 2116 15-19 0.28892640104341893 2116 20-24 0.3905891747975261 2116 25-29 0.282106969848563 2116 30-34 0.6857058926345445 2116 35-39 0.11501169417840629 2116 40-44 0.6520327193594113 2116 45-49 0.3321574825031277 2116 50-54 0.3999577998791679 2116 55-59 0.6522860796456627 2116 60-64 0.5820399808813477 2116 65-69 0.6944004608913374 2116 70-74 0.6696033481095256 2116 75-79 0.7443833770380976 2116 80-84 1.1269528423906507 2116 85-89 0.785893535660037 2116 90-94 1.017067303865261 2116 95-99 1.5395879316402201 2116 100-104 0.9003776409049067 2116 105-109 0.9179553077734184 2116 110-114 1.199773511478604 2116 115-119 1.1182678741970946 2116 120-124 1.4276465127762563 2116 125-129 1.4340685342706507 2116 130-134 1.4724813648350121 2116 135-139 1.9621736205215612 2116 140-144 2.129039021371792 2116 145-149 2.4921514237026514 2116 150-154 2.8421744715336352 2116 155-159 2.4528951070564524 2116 160-164 2.19964649303704 2116 165-169 2.12070315320684 2116 170-174 2.105312944009839 2116 175-179 2.595404022261512 2116 180-184 2.388267259029206 2116 185-189 1.9314922188450332 2116 190-194 2.1451236324468717 2116 195-199 1.528069606087442 2116 200-204 1.3214950618920298 2116 205-209 1.0434134430458606 2116 210-214 2.3582807200535107 2116 215-219 2.208805898140792 2116 220-224 1.6485224420006581 2116 225-229 1.309686993430514 2116 230-234 1.2249274722798873 2116 235-239 0.9918731642414453 2116 240-244 0.9685588095998803 2116 245-249 1.0065829665126511 2116 250-254 1.0114826498733738 2116 255-259 1.5476483290032483 2116 260-264 0.6702259547057086 2116 265-269 0.6192374773676779 2116 270-274 0.5693056201213587 2116 275-279 0.7232674047494232 2116 280-284 0.40346885159408963 2116 285-289 0.33983154214041456 2116 290-294 0.25487235013218346 2116 295-299 0.01603375262950202 2116 300-301 0.09827230512964213 1117 1 0.16489548917208197 1117 2 0.007395680412805916 1117 3 0.06757794763769454 1117 4 0.05040686003014372 1117 5 0.17397181726315836 1117 6 0.11491579907245608 1117 7 0.946479978964966 1117 8 0.198130643888625 1117 9 0.15717153977366394 1117 10-14 0.12315261527903942 1117 15-19 0.11003002539897011 1117 20-24 0.09999382533874979 1117 25-29 0.13223251758131482 1117 30-34 0.4623705430351066 1117 35-39 0.12486666368824473 1117 40-44 0.49413006984507746 1117 45-49 0.2429786772111342 1117 50-54 0.23604081611949113 1117 55-59 0.40545888537373287 1117 60-64 0.24913697969269322 1117 65-69 0.36307841161472965 1117 70-74 0.3417730731705291 1117 75-79 0.47771577257324793 1117 80-84 0.9092805043894288 1117 85-89 0.554299290458637 1117 90-94 0.4581725810901318 1117 95-99 0.9529800414751364 1117 100-104 0.5899206049567596 1117 105-109 0.4376434429851557 1117 110-114 0.6749776584222005 1117 115-119 0.5527938523792244 1117 120-124 0.8750536180042445 1117 125-129 0.9931093777263484 1117 130-134 0.8008282493522074 1117 135-139 0.9007767349493534 1117 140-144 1.3395380824013579 1117 145-149 1.6870050216826264 1117 150-154 2.0912193240765973 1117 155-159 1.544930914535854 1117 160-164 1.7522821162391047 1117 165-169 1.523661085924509 1117 170-174 1.0353918245601363 1117 175-179 1.3555841146815233 1117 180-184 1.1581525076981158 1117 185-189 1.2226811945584082 1117 190-194 -0.7400139073802485 1117 195-199 1.3323143151472756 1117 200-204 1.4880272717431282 1117 205-209 1.6188771256386865 1117 210-214 2.007785914283236 1117 215-219 1.7512538679249339 1117 220-224 1.4617784538731868 1117 225-229 1.4423030283728728 1117 230-234 1.3939491846524916 1117 235-239 1.162283462672276 1117 240-244 0.9601295277891779 1117 245-249 1.3213082737001702 1117 250-254 0.8701259255086757 1117 255-259 0.9990355083641855 1117 260-264 0.8571095575387968 1117 265-269 0.37557992375492866 1117 270-274 0.3752999089303799 1117 275-279 0.4170513852807325 1117 280-284 0.3494843815321236 1117 285-289 0.3655456168501079 1117 290-294 0.3452640801588416 1117 295-299 0.35297661258575275 1117 300-301 -0.140882058797013 2117 1 0.16342932375854957 2117 2 0.0882102167536516 2117 3 -0.04175789196130353 2117 4 0.044604855017610134 2117 5 0.06602695510777323 2117 6 0.21947720258122416 2117 7 0.9870689513960471 2117 8 0.3486945536630657 2117 9 0.32729685305687184 2117 10-14 0.18529547242189892 2117 15-19 0.3133132334190236 2117 20-24 0.24832715867208321 2117 25-29 0.21729517422292588 2117 30-34 0.6019444778721947 2117 35-39 0.3613027539138116 2117 40-44 0.5895260598200096 2117 45-49 0.24235962959209445 2117 50-54 0.32606337251046824 2117 55-59 0.567017782616837 2117 60-64 0.40151291954231283 2117 65-69 0.36335159457212995 2117 70-74 0.5036953789349496 2117 75-79 0.4739162738263829 2117 80-84 0.8906739880986976 2117 85-89 0.5068632002330702 2117 90-94 0.5171024056515279 2117 95-99 1.1381279111493328 2117 100-104 0.6519206049567572 2117 105-109 0.698034420428769 2117 110-114 1.0007119942618061 2117 115-119 0.9202825741837302 2117 120-124 1.1938907107360706 2117 125-129 0.9728437135659433 2117 130-134 0.9097881491015727 2117 135-139 1.3375261083829386 2117 140-144 2.2460769295191447 2117 145-149 2.009020059276608 2117 150-154 2.122953659916192 2117 155-159 2.2175800373428736 2117 160-164 2.1109763518280786 2117 165-169 2.1443979280297683 2117 170-174 1.7117652581440979 2117 175-179 2.2500327362354113 2117 180-184 1.8391625327607741 2117 185-189 1.5243829489443712 2117 190-194 1.6553444886097282 2117 195-199 1.7013669467262247 2117 200-204 2.0773455674824746 2117 205-209 1.6018846444356782 2117 210-214 1.8616706260626863 2117 215-219 1.9989255471229264 2117 220-224 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-0.3887217865296435 2110 275-279 -0.4395361713972239 2110 280-284 -0.4251626367770065 2110 285-289 -0.41128386142573525 2110 290-294 -0.38907558579994905 2110 295-299 -0.37712440331277364 2110 300-301 -0.4793355398278347 2109 1 -0.09290272257349841 2109 2 0.07757958612302218 2109 3 0.05541070520729363 2109 4 -0.07428832387557094 2109 5 -0.12591641683560084 2109 6 -0.20788444478041868 2109 7 0.5982529925800861 2109 8 0.040972545941052374 2109 9 -0.024041628281608496 2109 10-14 -0.13022576309933953 2109 15-19 -0.11632640622060819 2109 20-24 -0.2242339738890422 2109 25-29 -0.21291074598300241 2109 30-34 -4.053551017623299 2109 35-39 -0.21672041210936044 2109 40-44 -0.1872101049161543 2109 45-49 -0.45576649653403933 2109 50-54 -0.3868864344393401 2109 55-59 -0.22709547349641213 2109 60-64 -0.11284229281290692 2109 65-69 -0.25412845690792807 2109 70-74 -0.20645391321434658 2109 75-79 -0.6794067634966581 2109 80-84 -0.30449718307247053 2109 85-89 -0.5283414329715654 2109 90-94 -0.3849233343742071 2109 95-99 -0.18388238486097208 2109 100-104 -0.9591141440779865 2109 105-109 -0.48820496181061657 2109 110-114 -0.8242094986596911 2109 115-119 -0.737238661337507 2109 120-124 -0.5020140511686861 2109 125-129 -1.2319465052242684 2109 130-134 -0.9016661623527362 2109 135-139 -1.4861212532644217 2109 140-144 -0.6041573047150841 2109 145-149 -1.0374818712253209 2109 150-154 -1.1568095318469958 2109 155-159 -1.864178006415166 2109 160-164 -1.7349206880689607 2109 165-169 -1.6995557399238983 2109 170-174 -1.8958975475187074 2109 175-179 -1.6021937759911076 2109 180-184 -1.4708812252829837 2109 185-189 -1.4395063689449472 2109 190-194 -1.2471059618407239 2109 195-199 -2.4159380725787933 2109 200-204 -1.825323673186766 2109 205-209 -1.3327485601975297 2109 210-214 -1.6181234537313927 2109 215-219 -1.2337745815771974 2109 220-224 -1.3466039887197851 2109 225-229 -1.4238623851609624 2109 230-234 -1.2006149283326764 2109 235-239 -1.2749904653911255 2109 240-244 -1.10649310988609 2109 245-249 -0.8022133682425245 2109 250-254 -0.7444087219207098 2109 255-259 -0.7687403502011456 2109 260-264 -0.6459792455500057 2109 265-269 -0.6464582798622214 2109 270-274 -0.5661245982836007 2109 275-279 -0.33172427007387206 2109 280-284 -0.6737324443162809 2109 285-289 -0.9654440194026854 2109 290-294 -0.5347349037875126 2109 295-299 -0.3089975796515976 2109 300-301 -0.30398407058323507 2108 1 0.02392505936622058 2108 2 -0.062889641099936 2108 3 -0.18346009523067153 2108 4 -0.11558881946711352 2108 5 -0.06331917140253296 2108 6 -0.4086926623796856 2108 7 0.8314115242460858 2108 8 0.00924892466519367 2108 9 -0.24468431254369705 2108 10-14 -0.2711437599874884 2108 15-19 -0.10755919017727678 2108 20-24 -0.12247419171881546 2108 25-29 -0.17028122947289148 2108 30-34 0.21228918291838994 2108 35-39 -0.19843178766401337 2108 40-44 -0.11419391175070359 2108 45-49 -0.2191748409125367 2108 50-54 -0.23705283218036044 2108 55-59 -0.11031235242224824 2108 60-64 -0.07314166467942584 2108 65-69 -0.32843909483796807 2108 70-74 -0.27039026427756596 2108 75-79 -0.508750392840291 2108 80-84 -0.3730825861727993 2108 85-89 -0.5597689959289696 2108 90-94 -0.3546879283925364 2108 95-99 -0.26506647406744577 2108 100-104 -0.6284695869408949 2108 105-109 -0.6284335966287955 2108 110-114 -0.8306838834922843 2108 115-119 -0.8124050590785288 2108 120-124 -0.7335705543669775 2108 125-129 -0.9904572814589265 2108 130-134 -0.7351834216162629 2108 135-139 -0.9026810700108072 2108 140-144 -0.6218775836293915 2108 145-149 -0.9104181070346904 2108 150-154 -1.2029283585628399 2108 155-159 -1.7989292021738272 2108 160-164 -1.3081544228698583 2108 165-169 -1.3893046306056256 2108 170-174 -1.7671892550044426 2108 175-179 -1.2457330775527957 2108 180-184 -2.0883882845810895 2108 185-189 -1.444342708482779 2108 190-194 -1.4391750565217585 2108 195-199 -1.0543089594571455 2108 200-204 -1.1241711347207897 2108 205-209 -1.1552208992743438 2108 210-214 -1.1456410299429969 2108 215-219 -0.9731892361037957 2108 220-224 -0.9494464367114865 2108 225-229 -1.0436918094679974 2108 230-234 -0.7691988907599239 2108 235-239 -0.6973338642270619 2108 240-244 -0.6273915136203172 2108 245-249 -1.0628952388124553 2108 250-254 -0.6899332709900357 2108 255-259 -0.4325434390042364 2108 260-264 -0.5853211460859367 2108 265-269 -0.4457336213017431 2108 270-274 -0.5144879936619215 2108 275-279 -0.5053815627610145 2108 280-284 -0.7676657699660243 2108 285-289 -1.0567877351867008 2108 290-294 -0.7653758592418995 2108 295-299 -0.4864143087474311 2108 300-301 2.4979702024531267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 15.0 11 98.0 12 335.0 13 642.0 14 1195.0 15 2176.0 16 3248.0 17 4301.0 18 4867.0 19 6261.0 20 8342.0 21 10678.0 22 14105.0 23 18297.0 24 24536.0 25 32832.0 26 43947.0 27 53527.0 28 58885.0 29 53775.0 30 36143.0 31 15967.0 32 3876.0 33 421.0 34 8.0 35 2.0 36 1.0 37 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.49268848451997 18.308777582870952 13.070886692213682 35.1276472403954 2 52.73124691014126 21.18394603506817 13.395368913448822 12.689438141341745 3 52.30487777334427 19.984139770779535 15.472757797736906 12.238224658139284 4 52.550058848477086 18.849330331935526 15.702881693230042 12.89772912635734 5 32.44822212351404 19.110572398684003 35.96357166339173 12.477633814410224 6 31.41103339933397 18.834273152295843 36.073489074761405 13.681204373608779 7 15.333729839063844 54.037708196877645 17.079107912296948 13.549454051761565 8 30.52692600149066 17.731083790695166 37.42537285341083 14.316617354403347 9 13.966537927780747 16.713469400046677 38.33382269167162 30.986169980500954 10-14 28.00053202034727 36.63306406077078 21.174158868302378 14.192245050579578 15-19 22.051440344709032 37.044175255532885 18.985848760668638 21.91853563908944 20-24 26.494763865780303 30.589865012384532 25.464953159623672 17.4504179622115 25-29 20.020929479699156 25.11105924749235 38.16307427455764 16.704936998250858 30-34 19.504618789854472 27.603424002650062 31.630165553690137 21.261791653805325 35-39 20.062637867301074 30.151650894270993 27.325568847699138 22.460142390728794 40-44 20.633806881633003 28.615216283837874 28.760016161372814 21.990960673156312 45-49 22.48112206102675 29.02793357776155 25.7237860776298 22.767158283581903 50-54 22.468122695937822 29.895478078001208 26.363967165310264 21.272432060750702 55-59 22.431985464802587 26.5137861027251 28.731558091853813 22.3226703406185 60-64 22.464207829231505 25.196282884252952 29.485571457610277 22.85393782890527 65-69 19.9384663258725 27.254950675189033 27.603424002650062 25.203158996288405 70-74 20.54687676451324 24.890371184573418 29.141615284041144 25.4211367668722 75-79 20.065097206642225 24.75500713961268 29.851912638243732 25.327983015501367 80-84 24.847508413199122 24.79270027931068 26.399050393870727 23.960740913619468 85-89 21.703920638625178 26.942213054072845 26.043801335571835 25.310064971730146 90-94 20.451564817394054 29.34393358779967 29.305437398520883 20.899064196285394 95-99 21.884355841307364 29.274670561457132 26.53401291404107 22.30696068319443 100-104 22.453868565878928 27.7483242613826 25.830591671873943 23.967215500864533 105-109 23.72042832657015 27.24727151357279 23.031010261467923 26.001289898389135 110-114 20.530865963496378 28.73562353035653 29.082039043266803 21.651471462880288 115-119 24.8771208664905 26.651157771637795 25.909943008575066 22.561778353296642 120-124 18.78950313816719 27.413954492184068 27.552932260258334 26.24361010939041 125-129 20.139329102265854 23.377024249587812 31.80337331014528 24.68027333800106 130-134 26.393930952793234 27.787021213056583 25.28808148945621 20.530966344693976 135-139 25.199796226168875 26.676002118043268 23.774784745069404 24.349416910718453 140-144 20.647960630494303 27.329684476800654 26.991048506704207 25.03130638600084 145-149 21.46546510373142 26.52086297715575 28.832340814242087 23.181331104870747 150-154 23.167227546608245 24.441416278316908 25.644183787934683 26.747172387140168 155-159 22.098619507580036 24.626870540878986 29.749423435496297 23.52508651604468 160-164 25.421789244656583 29.008459625427562 24.860708540683245 20.70904258923261 165-169 21.80570717298943 24.748582742966416 27.706666064379483 25.739044019664675 170-174 23.140174813855616 28.274221355597884 25.38123524082704 23.204368589719458 175-179 20.272334189083043 28.233868114163535 27.6960758480329 23.797721848720517 180-184 20.028006354129808 29.69576968538023 26.300676820224805 23.975547140265157 185-189 21.940167787171784 27.029243552390202 26.85488141216269 24.175707248275323 190-194 20.253362142737043 26.338570722318 26.209580883404733 27.19848625154022 195-199 20.80054005084308 29.821898660161967 26.778892845581094 22.598668443413864 200-204 22.766054090408325 26.471375046739997 26.250887746216257 24.511683116635425 205-209 21.030613755737413 27.01408599155292 27.453303921642437 24.50199633106723 210-214 23.953764420386417 25.389566880227665 27.51272959062038 23.143939108765537 215-219 23.94322439463864 25.246071958261496 27.757509140962807 23.053194506137057 220-224 18.816555870919817 26.223784822864832 28.67649900497138 26.283160301243974 225-229 22.466315834381064 27.56302057061692 25.353128505499633 24.617535089502386 230-234 21.500347569896682 26.11120730975881 28.14648628165458 24.241958838689925 235-239 23.26434635528419 25.363066244061827 24.372705348561162 26.999882052092822 240-244 22.808716099387425 30.190950133130563 22.488249126056196 24.512084641425815 245-249 21.688411743596305 28.24219975356416 22.64810618323082 27.421282319608714 250-254 22.33546894331223 25.730862952060452 24.391978538499952 27.54168956612737 255-259 22.34344924852126 26.031404257668495 25.926004000190723 25.69914249361952 260-264 21.73368366371295 27.642673050910833 24.903621502656335 25.720021782719876 265-269 21.44694477277461 27.247723228961984 25.328133587297764 25.97719841096564 270-274 20.146907882684495 28.968206765190814 23.543757418797885 27.34112793332681 275-279 21.68484821108158 27.46349261319862 22.276093464933084 28.57556571078671 280-284 19.266865923343897 28.512174984503652 25.303138669095894 26.91782042305656 285-289 21.352787209427802 26.921333764972484 26.22413615705642 25.501742868543293 290-294 20.911310702392335 29.736925976395362 23.48051726431122 25.871246056901082 295-299 20.38415884320708 30.38829956760799 21.481526095347082 27.74601549383785 300-301 16.391873138242477 30.30658927276332 27.87548214343971 25.42605544555449 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.25 3 0.0 4 0.5 5 0.25 6 1.25 7 1.75 8 1.5 9 3.0 10 1.75 11 1.5 12 2.75 13 1.75 14 3.25 15 2.0 16 2.25 17 2.5 18 1.25 19 0.75 20 0.25 21 2.0 22 1.5 23 1.0 24 1.75 25 1.25 26 1.25 27 1.0 28 1.25 29 0.5 30 1.0 31 3.25 32 8.75 33 27.0 34 79.25 35 197.75 36 592.5 37 1554.0 38 3076.0 39 4342.75 40 5208.75 41 6512.25 42 8917.0 43 11832.5 44 14013.0 45 13474.25 46 10996.5 47 9001.5 48 7752.25 49 6818.0 50 6031.5 51 5014.75 52 3347.25 53 1882.5 54 1151.75 55 984.0 56 1009.75 57 975.75 58 974.25 59 1027.0 60 1162.5 61 1261.5 62 1207.75 63 945.5 64 615.75 65 372.5 66 212.25 67 121.0 68 82.5 69 54.0 70 33.25 71 21.25 72 13.75 73 7.75 74 5.5 75 4.75 76 2.0 77 4.25 78 2.5 79 2.0 80 3.0 81 1.75 82 1.5 83 1.25 84 1.5 85 0.5 86 0.75 87 0.5 88 0.75 89 1.25 90 0.25 91 0.25 92 0.5 93 0.5 94 0.75 95 0.25 96 0.0 97 0.25 98 0.25 99 0.75 100 0.6666666666666666 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 398481.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.465389153154389 #Duplication Level Percentage of deduplicated Percentage of total 1 55.37859476741678 8.564515188329398 2 8.770586375794123 2.712810628060202 3 5.7243167480885075 2.6558635843532397 4 4.571467439067238 2.8279809178459567 5 3.7378976142975167 2.8904020609879235 6 3.199026295970252 2.968451194701239 7 2.7189269204940865 2.9434484033100095 8 2.1538002150630295 2.6647486787277894 9 1.7278948346769665 2.405030943060418 >10 11.38140708015475 31.873785562652863 >50 0.49950234983799513 5.078935420039441 >100 0.08615372088554708 2.7736680342798867 >500 0.013446836867732034 1.565423153319338 >1k 0.026893673734921852 10.980577784515576 >5k 0.005042563825297847 5.610516093918223 >10k+ 0.005042563825297847 11.483842351898502 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 21893 5.494113897525854 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 11729 2.943427666563776 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 10555 2.648808851614004 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 9540 2.394091562709389 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 6909 1.7338342355093468 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG 5134 1.2883926711687634 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 4613 1.1576461612975275 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 4176 1.0479797029218456 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 3694 0.9270203598164029 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 3434 0.8617725813777821 No Hit GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 3311 0.8309053631164347 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 2916 0.7317789304885302 No Hit GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 2872 0.7207369987527636 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 2688 0.6745616478577398 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 2592 0.6504701604342491 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC 2403 0.6030400445692518 No Hit GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 2141 0.5372903601426416 No Hit GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 1711 0.4293805727249229 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 1651 0.41432339308524124 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT 1512 0.3794409269199786 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 1328 0.33326557602495477 No Hit GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 1199 0.30089263979963915 No Hit GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA 968 0.24292249818686462 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 846 0.21230623291951184 No Hit GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 794 0.1992566772317877 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 782 0.1962452413038514 Illumina Single End PCR Primer 1 (96% over 32bp) GGGGGGAGCGAAAGCATGGGTAGCAAACAGGATTAGATACCCTGGTAGTC 735 0.1844504505861007 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT 672 0.16864041196443494 No Hit CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG 659 0.16537802304250393 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG 566 0.1420393946009973 No Hit GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC 491 0.12321792005139517 No Hit GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 491 0.12321792005139517 No Hit GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG 489 0.12271601406340579 No Hit GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC 486 0.1219631550814217 No Hit GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC 482 0.12095934310544293 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 471 0.11819886017150127 No Hit GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG 451 0.11317980029160739 No Hit GGGGGGAGCGAAAGCAGGGGTAGCGAACAGGATTAGATACCCTGGTAGTC 440 0.11041931735766575 No Hit GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 408 0.1023888215498355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 2.5095299399469485E-4 0.0 0.0 9 2.5095299399469485E-4 0.0 0.0 10-14 0.0016060991615660472 0.0 0.0 15-19 0.0021080051495554366 0.0 0.0 20-24 0.0025095299399469483 0.0 0.0 25-29 0.0028608641315395213 0.0 0.0 30-34 0.008381829999422809 0.0 0.0 35-39 0.00883354538861326 0.0 0.0 40-44 0.010389453951380367 0.0 0.0 45-49 0.014153748861300788 0.0 0.0 50-54 0.021732529279940575 0.0 0.0 55-59 0.02534625239346418 0.0 0.0 60-64 0.030867218261347463 0.0 0.0 65-69 0.03663913712322545 0.0 0.0 70-74 0.0439167739490716 0.0 0.0 75-79 0.05360355951726683 0.0 0.0 80-84 0.0694135981389326 0.0 0.0 85-89 0.08582592394618564 0.0 0.0 90-94 0.12136086789583445 0.0 0.0 95-99 0.151324655378801 0.0 0.0 100-104 0.1915775156155501 0.0 0.0 105-109 0.23343647501386516 0.0 0.0 110-114 0.27117980531066727 0.0 0.0 115-119 0.31253685872099296 0.0 0.0 120-124 0.36348031650191603 0.0 0.0 125-129 0.4257166590126003 0.0 0.0 130-134 0.5026588469713739 0.0 0.0 135-139 0.5868284811571944 0.0 0.0 140-144 0.6863564385754903 0.0 0.0 145-149 0.8168519954527318 0.0 0.0 150-154 0.9713888491546647 0.0 0.0 155-159 1.1713481947696376 0.0 0.0 160-164 1.3801912763720228 0.0 0.0 165-169 1.6100642188711631 0.0 0.0 170-174 1.872410478793217 0.0 0.0 175-179 2.177117604101576 0.0 0.0 180-184 2.5526436643152373 0.0 0.0 185-189 3.136008994155305 0.0 0.0 190-194 3.546869235923419 0.0 0.0 195-199 3.972937229127612 0.0 0.0 200-204 4.379782222991812 0.0 0.0 205-209 4.8117727068542795 0.0 0.0 210-214 5.245821005267503 0.0 0.0 215-219 5.68544046014741 0.0 0.0 220-224 6.154019890534304 0.0 0.0 225-229 6.608847096850289 0.0 0.0 230-234 7.020259435205192 0.0 0.0 235-239 7.398445597155197 0.0 0.0 240-244 7.739992621981976 0.0 0.0 245-249 8.017797586334103 0.0 0.0 250-254 8.256052358832665 0.0 0.0 255-259 8.446575871873439 0.0 0.0 260-264 8.613911328269102 0.0 0.0 265-269 8.756502819456887 0.0 0.0 270-274 8.865918324838576 0.0 0.0 275-279 8.935131160582312 0.0 0.0 280-284 8.988082242315192 0.0 0.0 285-289 9.02411909225283 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 8010 0.0 290.21222 1 GGGTTAT 8040 0.0 289.12936 2 GTTATTA 8140 0.0 285.21497 4 TTATTAG 8120 0.0 284.28265 5 GGTTATT 8230 0.0 282.09598 3 TTAGTAG 8255 0.0 281.9564 8 GGGGGGA 6640 0.0 281.67166 1 TATTAGT 8305 0.0 278.12762 6 ATTAGTA 8310 0.0 277.9603 7 TAGTAGA 8430 0.0 275.75327 9 GGGGAGC 6975 0.0 267.50894 3 GGGAGCG 5145 0.0 265.18463 4 GGAGCGA 5180 0.0 261.96912 5 GAGCGAA 5230 0.0 258.90057 6 CGAAAGC 5315 0.0 257.8128 9 GCGAAAG 5435 0.0 252.12051 8 AGCGAAA 5390 0.0 251.21521 7 GGGGGAG 7725 0.0 243.06473 2 GGGAGCA 2335 0.0 212.88008 4 CAAAAGC 2630 0.0 175.54182 9 >>END_MODULE