FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30429_flu.3520000003bb0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30429_flu.3520000003bb0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398481
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC218935.494113897525854No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG117292.943427666563776No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA105552.648808851614004No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT95402.394091562709389No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA69091.7338342355093468No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG51341.2883926711687634No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT46131.1576461612975275No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG41761.0479797029218456No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA36940.9270203598164029No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT34340.8617725813777821No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG33110.8309053631164347No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA29160.7317789304885302No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC28720.7207369987527636No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26880.6745616478577398No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG25920.6504701604342491No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC24030.6030400445692518No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC21410.5372903601426416No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA17110.4293805727249229No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16510.41432339308524124No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT15120.3794409269199786No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG13280.33326557602495477No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11990.30089263979963915No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA9680.24292249818686462No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8460.21230623291951184No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7940.1992566772317877No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT7820.1962452413038514Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCATGGGTAGCAAACAGGATTAGATACCCTGGTAGTC7350.1844504505861007No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT6720.16864041196443494No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG6590.16537802304250393No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG5660.1420393946009973No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC4910.12321792005139517No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG4910.12321792005139517No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG4890.12271601406340579No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4860.1219631550814217No Hit
GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC4820.12095934310544293No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4710.11819886017150127No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG4510.11317980029160739No Hit
GGGGGGAGCGAAAGCAGGGGTAGCGAACAGGATTAGATACCCTGGTAGTC4400.11041931735766575No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4080.1023888215498355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA80100.0290.212221
GGGTTAT80400.0289.129362
GTTATTA81400.0285.214974
TTATTAG81200.0284.282655
GGTTATT82300.0282.095983
TTAGTAG82550.0281.95648
GGGGGGA66400.0281.671661
TATTAGT83050.0278.127626
ATTAGTA83100.0277.96037
TAGTAGA84300.0275.753279
GGGGAGC69750.0267.508943
GGGAGCG51450.0265.184634
GGAGCGA51800.0261.969125
GAGCGAA52300.0258.900576
CGAAAGC53150.0257.81289
GCGAAAG54350.0252.120518
AGCGAAA53900.0251.215217
GGGGGAG77250.0243.064732
GGGAGCA23350.0212.880084
CAAAAGC26300.0175.541829
Produced by FastQC (version 0.11.2)