##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30427_flu.3520000003bb18.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350507
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.162849814697	34.0	34.0	34.0	34.0	34.0
2	33.37217801641622	34.0	34.0	34.0	34.0	34.0
3	33.401449899716695	34.0	34.0	34.0	34.0	34.0
4	33.39479097421735	34.0	34.0	34.0	34.0	34.0
5	33.3197282793211	34.0	34.0	34.0	34.0	34.0
6	36.774132898915	38.0	38.0	38.0	35.0	38.0
7	36.08864016981116	38.0	38.0	38.0	34.0	38.0
8	36.71890718302345	38.0	38.0	38.0	34.0	38.0
9	36.78149937091128	38.0	38.0	38.0	36.0	38.0
10-14	36.807285446510335	38.0	38.0	38.0	35.8	38.0
15-19	36.69379670020855	38.0	38.0	38.0	35.6	38.0
20-24	36.776343411115896	38.0	38.0	38.0	35.8	38.0
25-29	36.67993848910292	38.0	38.0	38.0	35.0	38.0
30-34	36.32516611651123	38.0	37.4	38.0	33.0	38.0
35-39	36.57539050575309	38.0	38.0	38.0	34.4	38.0
40-44	36.116938606076346	38.0	37.0	38.0	33.0	38.0
45-49	36.39889474389955	38.0	37.0	38.0	34.0	38.0
50-54	36.33838525336155	38.0	37.0	38.0	34.0	38.0
55-59	35.97388354583504	38.0	37.0	38.0	32.8	38.0
60-64	36.14076124014641	38.0	37.0	38.0	33.0	38.0
65-69	36.00060540873649	38.0	37.0	38.0	32.4	38.0
70-74	35.850012695894804	38.0	37.0	38.0	31.6	38.0
75-79	35.88102948015304	38.0	37.0	38.0	31.8	38.0
80-84	35.13580670286185	38.0	36.4	38.0	28.0	38.0
85-89	35.331943156627396	38.0	37.0	38.0	28.0	38.0
90-94	35.26184584045397	38.0	36.2	38.0	28.0	38.0
95-99	34.342557495285405	38.0	34.8	38.0	23.6	38.0
100-104	34.62138445166573	38.0	35.2	38.0	24.0	38.0
105-109	34.4139198361231	37.8	34.8	38.0	23.8	38.0
110-114	33.88894544188846	37.0	34.0	38.0	23.0	38.0
115-119	33.88093361901474	37.0	34.0	38.0	23.2	38.0
120-124	33.40041254525587	37.0	32.8	38.0	21.8	38.0
125-129	32.93328007714539	37.0	31.0	38.0	21.0	38.0
130-134	32.57656994011532	36.8	30.6	38.0	20.2	38.0
135-139	31.823910506780173	36.0	28.0	38.0	16.8	38.0
140-144	30.71546417047306	35.4	26.0	37.6	13.6	38.0
145-149	30.474243310404642	35.0	25.4	37.6	10.8	38.0
150-154	29.427463074917192	34.0	23.6	37.0	10.2	38.0
155-159	29.18336695130197	33.8	23.2	37.0	10.0	37.0
160-164	28.383496477959067	32.6	21.4	37.0	10.0	37.2
165-169	27.297026307605837	30.4	20.2	36.0	9.4	37.0
170-174	26.71050050355628	29.2	20.0	35.4	9.2	37.0
175-179	25.966926195482547	28.0	19.2	35.2	9.0	37.0
180-184	25.09669535843792	26.4	17.2	35.0	8.8	36.6
185-189	24.385613411429734	25.4	15.8	35.0	8.2	36.2
190-194	23.52592729959744	24.6	15.2	33.8	8.0	36.0
195-199	22.69717009931328	23.4	13.6	32.4	8.0	36.0
200-204	21.9223193830651	23.0	11.6	31.0	8.0	35.4
205-209	21.06898749525687	21.4	9.4	29.6	8.0	35.0
210-214	19.86056027411721	20.0	9.0	28.0	8.0	34.4
215-219	19.420612427141258	19.4	9.0	26.6	8.0	34.0
220-224	18.8260884946663	18.2	9.0	26.0	8.0	33.2
225-229	18.171472181725328	16.8	9.0	25.4	8.0	32.4
230-234	17.29136022961025	15.6	9.0	24.2	7.8	31.0
235-239	16.3523861149706	14.6	8.6	22.8	7.2	29.0
240-244	15.673870136687714	13.8	8.0	21.8	7.0	28.6
245-249	15.033316880975272	12.8	8.0	20.6	7.0	27.4
250-254	14.380284559224208	12.2	8.0	19.6	7.0	26.0
255-259	13.816460156287892	11.2	8.0	18.4	7.0	25.0
260-264	13.286524377544527	10.6	8.0	17.4	7.0	23.8
265-269	12.853731309217793	9.8	8.0	16.4	7.0	22.8
270-274	12.400980294259458	9.0	8.0	15.6	7.0	21.8
275-279	11.988689526885342	9.0	7.0	14.2	7.0	21.0
280-284	11.828093590142279	8.6	7.0	14.2	7.0	20.8
285-289	11.480275714892997	8.0	7.2	13.2	7.0	19.6
290-294	11.160441874199375	8.0	7.0	13.0	7.0	19.0
295-299	10.865753323043476	8.0	7.0	13.0	7.0	19.0
300-301	10.550444071017127	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.0824340173694651
1101	2	-0.07717898131827639
1101	3	0.019007114289827598
1101	4	0.0920688015624691
1101	5	0.05466301459498624
1101	6	0.09084367512386393
1101	7	0.7948387523351599
1101	8	0.2563738270999707
1101	9	0.09431254375739684
1101	10-14	0.021476655708127623
1101	15-19	0.08902197790902733
1101	20-24	0.07199092445377886
1101	25-29	-0.11460659311900656
1101	30-34	0.41256734807512174
1101	35-39	0.10662081761696385
1101	40-44	0.37971034419969385
1101	45-49	0.17591191187420208
1101	50-54	0.3220618114187914
1101	55-59	0.23133346442503466
1101	60-64	0.2753663828455828
1101	65-69	0.2795820818558994
1101	70-74	0.14443903437820182
1101	75-79	0.3562878296810794
1101	80-84	0.5391733612772853
1101	85-89	0.24461343149616255
1101	90-94	0.41676842315062856
1101	95-99	0.7913743536251232
1101	100-104	0.30778809698733056
1101	105-109	0.565090169353283
1101	110-114	0.5665211038680766
1101	115-119	0.43088674978680785
1101	120-124	0.8589277227486392
1101	125-129	0.5211530184776763
1101	130-134	0.801826275713637
1101	135-139	0.5266949506194756
1101	140-144	1.2503049373744126
1101	145-149	1.2642623103878172
1101	150-154	1.087639960069545
1101	155-159	0.7381405321508687
1101	160-164	1.3352175722100768
1101	165-169	1.0724334217832912
1101	170-174	0.43727630761090097
1101	175-179	1.0033431357856024
1101	180-184	0.743635474081632
1101	185-189	0.5633877638744131
1101	190-194	1.1031757691322284
1101	195-199	1.0245632490269934
1101	200-204	1.0785461239309697
1101	205-209	0.7635227969806131
1101	210-214	1.0802309400986552
1101	215-219	1.3903188825339114
1101	220-224	0.9815171145372226
1101	225-229	0.5222441252651038
1101	230-234	0.742362444773498
1101	235-239	0.5202294830698371
1101	240-244	0.172785276746243
1101	245-249	-0.04641663219004499
1101	250-254	0.2960410819596948
1101	255-259	0.3146437326955347
1101	260-264	0.3530493708432587
1101	265-269	0.48240647387347835
1101	270-274	0.20555587662691366
1101	275-279	0.22413014477015558
1101	280-284	-0.002612179436322748
1101	285-289	0.23355441998406334
1101	290-294	-0.00630099583593946
1101	295-299	0.059912213753666066
1101	300-301	-0.2821581579372019
1102	1	-0.12296122121525599
1102	2	-0.0298211908768522
1102	3	0.008971705604260194
1102	4	-0.013134966402752468
1102	5	0.01340004585438237
1102	6	-0.014016971712521809
1102	7	0.8471806424727291
1102	8	0.24802218807754883
1102	9	0.1628766348932018
1102	10-14	0.19386990789508474
1102	15-19	0.1659150691474096
1102	20-24	0.08684913053162546
1102	25-29	0.1144172095828111
1102	30-34	0.5100201158599376
1102	35-39	0.20581182996578917
1102	40-44	0.5046177545548858
1102	45-49	0.19244539802024718
1102	50-54	0.1637704838937566
1102	55-59	0.3022413912305737
1102	60-64	0.21582673113122297
1102	65-69	0.2247576926645749
1102	70-74	0.2852524083200976
1102	75-79	0.3149137887400073
1102	80-84	0.8075768575754552
1102	85-89	0.29017425767523974
1102	90-94	0.43298284202017356
1102	95-99	1.246147805246821
1102	100-104	0.8131284059378387
1102	105-109	0.8761361935698488
1102	110-114	0.8827776169791903
1102	115-119	0.825326772567216
1102	120-124	1.3145069784233954
1102	125-129	1.094809805378219
1102	130-134	1.0634819589668538
1102	135-139	1.006688866409771
1102	140-144	1.628332450736682
1102	145-149	1.8354606520869403
1102	150-154	1.6805327541721056
1102	155-159	1.3914056657381906
1102	160-164	1.709462263560244
1102	165-169	1.3237650501229794
1102	170-174	1.7664702559446397
1102	175-179	1.3715283787472003
1102	180-184	1.3887569637225567
1102	185-189	1.6401556335874297
1102	190-194	1.4959935718128143
1102	195-199	1.5431819919302256
1102	200-204	1.7088461957387935
1102	205-209	1.2620912390338184
1102	210-214	1.4318621803931002
1102	215-219	2.009797550657961
1102	220-224	1.4381589845120004
1102	225-229	1.464807063230925
1102	230-234	0.843416215745453
1102	235-239	1.0739335994621797
1102	240-244	0.7830675911513172
1102	245-249	0.19367618738129977
1102	250-254	0.8238186828709111
1102	255-259	0.3041817218642269
1102	260-264	0.4828213190991768
1102	265-269	0.48226498062447476
1102	270-274	0.38069268291204494
1102	275-279	0.4223133714529528
1102	280-284	0.03543111042320923
1102	285-289	0.16016611652349333
1102	290-294	-0.08606569256284935
1102	295-299	0.2159898793980446
1102	300-301	-0.016743352791092292
1103	1	0.004382702887767209
1103	2	0.04715113658097181
1103	3	0.10903926863066005
1103	4	0.20675261721090976
1103	5	0.16613139615982675
1103	6	0.112279902347872
1103	7	0.9674003814884315
1103	8	0.4589461743668508
1103	9	0.36548081814110844
1103	10-14	0.23047987114221513
1103	15-19	0.30231243878792213
1103	20-24	0.2698473017313745
1103	25-29	0.15613295672022787
1103	30-34	0.5615491272819924
1103	35-39	0.3351309998248908
1103	40-44	0.5985742241568204
1103	45-49	0.1816996932246866
1103	50-54	0.1010543087392648
1103	55-59	0.35201942369619843
1103	60-64	0.33410164543936816
1103	65-69	0.3563237753178541
1103	70-74	0.16823324659684147
1103	75-79	0.35350112014195645
1103	80-84	0.8860115177617445
1103	85-89	0.47440978733752814
1103	90-94	0.32973734062115767
1103	95-99	1.129638019219982
1103	100-104	0.6231149994524969
1103	105-109	0.9436539421292736
1103	110-114	0.817968049205696
1103	115-119	0.78094034035486
1103	120-124	1.0173414419340574
1103	125-129	0.9170801353051132
1103	130-134	1.1441628244810644
1103	135-139	1.0565984875969683
1103	140-144	1.3917840370528758
1103	145-149	1.6031690627993918
1103	150-154	1.8807803673578647
1103	155-159	0.8689015182173279
1103	160-164	0.790523038448022
1103	165-169	1.5710400670137297
1103	170-174	2.3736107127555925
1103	175-179	1.9351759332132588
1103	180-184	1.0870438342102489
1103	185-189	1.6703545377222113
1103	190-194	1.4680203564848533
1103	195-199	1.6929448138715841
1103	200-204	1.7319223297187527
1103	205-209	1.1834584882989425
1103	210-214	1.747969418126516
1103	215-219	1.628261004720411
1103	220-224	1.6143145421899554
1103	225-229	0.806274135983216
1103	230-234	1.427892991397293
1103	235-239	1.0773570286218188
1103	240-244	0.9311989959316715
1103	245-249	1.420678759021099
1103	250-254	1.2002211462683778
1103	255-259	0.59738756978021
1103	260-264	0.4835959946374686
1103	265-269	0.5707237300363932
1103	270-274	0.6784390491885439
1103	275-279	0.41941417478087395
1103	280-284	0.5832399105958306
1103	285-289	0.37053191194548063
1103	290-294	0.1285328734030724
1103	295-299	0.18981575073115842
1103	300-301	-0.08172943339272187
2116	1	0.18923595594927178
2116	2	-0.00808933684038493
2116	3	-0.09469610798560524
2116	4	-0.0072037349817364316
2116	5	0.02937738826947367
2116	6	0.1397456385910587
2116	7	1.0724960463535425
2116	8	0.24272761606715676
2116	9	0.320674542343518
2116	10-14	0.1392808106874881
2116	15-19	0.26279543803536853
2116	20-24	0.31058388698598804
2116	25-29	0.26367007103916507
2116	30-34	0.6407281653882961
2116	35-39	-0.0024373566550011105
2116	40-44	0.4194095528836499
2116	45-49	0.27757846451838475
2116	50-54	0.4306791747628438
2116	55-59	0.5442063749629895
2116	60-64	0.3943668617400249
2116	65-69	0.6049919926903229
2116	70-74	0.5249268225700376
2116	75-79	0.5819111906078476
2116	80-84	1.0630008680616143
2116	85-89	0.7304155796797787
2116	90-94	0.8489948261973197
2116	95-99	1.3238511500493786
2116	100-104	0.9482366614442483
2116	105-109	0.7545056900939713
2116	110-114	0.9632347477986585
2116	115-119	1.037392872794257
2116	120-124	1.303898875059872
2116	125-129	1.1455688812858185
2116	130-134	1.2653406767535316
2116	135-139	1.799584964530208
2116	140-144	2.076839109913241
2116	145-149	1.9887066788172767
2116	150-154	2.334626801874748
2116	155-159	2.3303689876022844
2116	160-164	1.9281821568261606
2116	165-169	1.6982834594107103
2116	170-174	1.554658351349925
2116	175-179	2.3713767040052574
2116	180-184	2.315261890811005
2116	185-189	1.7793862587776097
2116	190-194	1.8149178046616328
2116	195-199	1.0581549573118636
2116	200-204	1.071179131160001
2116	205-209	1.1793210683997373
2116	210-214	1.906488494088297
2116	215-219	2.038060781236336
2116	220-224	1.5114899771857395
2116	225-229	0.8594104384620671
2116	230-234	1.439667181267538
2116	235-239	0.9457782732912676
2116	240-244	1.0863213420583246
2116	245-249	1.2724791740629495
2116	250-254	1.191606291419001
2116	255-259	1.7525945205909057
2116	260-264	0.7984332845510416
2116	265-269	0.7461343250057197
2116	270-274	0.6430649414144902
2116	275-279	0.5943588738755352
2116	280-284	0.34571807528990917
2116	285-289	0.3534201014964591
2116	290-294	0.17021242461057007
2116	295-299	0.11595289697639721
2116	300-301	0.1453092886432632
1117	1	0.2564154786697159
1117	2	-0.014720658022270072
1117	3	0.21112319111112043
1117	4	0.07001113322349539
1117	5	0.14342086552592548
1117	6	0.1759043005449925
1117	7	0.985163821898162
1117	8	0.4265195166377609
1117	9	0.3447602955719731
1117	10-14	0.17984010100560255
1117	15-19	0.2175213372660778
1117	20-24	0.36444809563985814
1117	25-29	0.3339181824814048
1117	30-34	0.5723852448809836
1117	35-39	0.39161790242970795
1117	40-44	0.6048682356290485
1117	45-49	0.35041187870740487
1117	50-54	0.35708525511029166
1117	55-59	0.5167055086520875
1117	60-64	0.4923470867498452
1117	65-69	0.48097438925280045
1117	70-74	0.562136839434217
1117	75-79	0.7026455912362195
1117	80-84	1.065437627365796
1117	85-89	0.765251211624701
1117	90-94	0.6852477427773565
1117	95-99	1.1804020775795792
1117	100-104	0.7093919736626972
1117	105-109	0.7118550097511473
1117	110-114	0.8386585470917538
1117	115-119	0.998935599247929
1117	120-124	1.2230545107010542
1117	125-129	1.1242225186365218
1117	130-134	1.0374641780356697
1117	135-139	1.528711492125101
1117	140-144	1.3139460935341951
1117	145-149	2.0612326103902667
1117	150-154	2.3466733747488213
1117	155-159	1.6651861382479787
1117	160-164	1.928696629993624
1117	165-169	1.545647564107277
1117	170-174	1.4101521485638706
1117	175-179	1.596329230167843
1117	180-184	1.44071225697175
1117	185-189	1.3800989438793714
1117	190-194	-0.6532458472430918
1117	195-199	1.3333713040210426
1117	200-204	1.6679830214734856
1117	205-209	1.650167049872234
1117	210-214	2.0432237725783757
1117	215-219	2.1920051525256383
1117	220-224	1.709686202380368
1117	225-229	1.4627379963893858
1117	230-234	1.362372150426868
1117	235-239	1.1061779313872364
1117	240-244	1.1598355611079239
1117	245-249	1.0746715181847417
1117	250-254	1.092105055482115
1117	255-259	0.8675699173612994
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2108	245-249	-1.115456396159047
2108	250-254	-0.8901913732323106
2108	255-259	-0.5958321189992528
2108	260-264	-0.47283684975458584
2108	265-269	-0.474201208293767
2108	270-274	-0.48977163033056037
2108	275-279	-0.26917091797381865
2108	280-284	-0.8485270901690836
2108	285-289	-1.2723661171771
2108	290-294	-0.9181306778032372
2108	295-299	-0.44623291554956346
2108	300-301	2.440337442056288
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	7.0
11	65.0
12	238.0
13	531.0
14	980.0
15	1708.0
16	2596.0
17	3628.0
18	4767.0
19	5448.0
20	6933.0
21	8812.0
22	11569.0
23	15185.0
24	20684.0
25	27736.0
26	37062.0
27	47263.0
28	53469.0
29	49425.0
30	33523.0
31	14725.0
32	3724.0
33	412.0
34	10.0
35	4.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.920067216917207	20.03925741853943	14.079889987931768	34.96078537661159
2	49.29915807672886	22.74276975923448	14.548354241142118	13.40971792289455
3	49.176193342786306	21.408987552317072	16.484692174478685	12.930126930417938
4	49.275192792155366	20.110297369239415	16.96371256494164	13.650797273663578
5	30.29754042002014	20.134262653812907	36.28229964023543	13.285897285931522
6	29.224238032336014	20.002168287651887	36.38129908960448	14.392294590407609
7	15.99682745280408	51.40781781818908	18.412185776603607	14.183168952403232
8	28.346081533321733	18.910036033517162	37.98240833991903	14.761474093242075
9	14.751773859009948	17.805921137095694	38.73389119190202	28.708413811992344
10-14	27.38204943125244	35.606079193853475	22.187060458136358	14.824810916757725
15-19	21.484706439529024	36.828594008108254	19.99320983603751	21.69348971632521
20-24	26.029551478287168	30.77610432887218	26.157765750755335	17.03657844208532
25-29	19.22689133169951	25.069057108702538	38.94056324124768	16.763488318350277
30-34	19.08538203231319	27.655995458007972	31.930032781085682	21.328589728593155
35-39	19.952411792061213	30.371604561392495	27.47836705115732	22.197616595388965
40-44	20.25106488600798	28.660426182644	29.646825883648543	21.441683047699474
45-49	21.731548870635965	29.872042498437978	25.573126927564928	22.823281703361133
50-54	22.049145951436063	30.26923856014288	26.503550571029965	21.178064917391094
55-59	21.94432636152773	26.99432536297421	29.19736267749289	21.863985598005176
60-64	21.94392694011817	25.411646557700703	29.112114736652906	23.53231176552822
65-69	19.461637000116973	27.820043536933643	28.0549603859552	24.663359076994183
70-74	20.444727209442323	25.949895437181	29.295392103438733	24.309985249937945
75-79	19.953895357296716	25.480347040144707	30.18256411426876	24.38319348828982
80-84	23.97093353342444	24.8580484840531	27.022513102448737	24.14850488007372
85-89	20.788457862467798	27.817989369684486	26.26321300287869	25.13033976496903
90-94	19.777294034070646	30.365270879040935	29.13642238243459	20.721012704453834
95-99	22.01490983061679	30.329037651173874	26.299731531752574	21.356320986456762
100-104	22.66779265464028	28.087484700733512	26.149492021557347	23.09523062306887
105-109	23.397706750507123	27.456741234839814	23.173802520349092	25.971749494303964
110-114	21.019494617796504	28.486906110291667	29.219273794817223	21.274325477094607
115-119	24.44915508106828	27.06108579857178	26.300872735779883	22.18888638458005
120-124	18.500629088720054	28.037956445948296	27.611203199936092	25.850211265395558
125-129	20.013751508529072	23.594222084009733	32.024353293942774	24.367673113518418
130-134	25.74293808682851	28.374269272796266	25.424770404014758	20.45802223636047
135-139	25.057930369436278	26.755300179454334	23.945370563212716	24.241398887896676
140-144	20.0938069710448	28.617288670411718	27.143480729343494	24.14542362919999
145-149	20.483356965766735	26.814756909277133	29.140416596530166	23.561469528425967
150-154	22.95885674180544	24.42638806072347	25.870809998088482	26.74394519938261
155-159	22.202238471699566	25.2709361011335	29.804768521028112	22.72205690613882
160-164	25.076474934880043	29.2092311993769	25.31287534913711	20.401418516605947
165-169	20.53391230417652	24.745982248571355	28.620541101889547	26.099564345362573
170-174	22.487938899936378	28.47355402317215	26.016827053382674	23.021680023508804
175-179	19.68679655470504	28.34847806177908	28.86070748943673	23.10401789407915
180-184	19.430025648560516	29.691675201921786	26.51028367479109	24.368015474726608
185-189	21.177893736787	27.241909576698898	27.79271170047959	23.787484986034517
190-194	19.256505576208177	26.57544642475043	27.14079889987932	27.02724909916207
195-199	20.451060891793887	29.684827977757934	27.52156162359097	22.34254950685721
200-204	21.801961159120932	26.226352112796608	27.406699438242317	24.564987289840147
205-209	20.34019292054082	26.822916518072393	28.19498612010602	24.641904441280772
210-214	23.15588561712034	25.948183631140033	27.799673045046173	23.096257706693446
215-219	22.736949618695203	25.89768535293161	28.73312087918358	22.632244149189603
220-224	18.236839777807575	25.994345334044684	29.37767291380771	26.391141974340027
225-229	21.294011246565688	27.54227447668663	26.775784791744528	24.387929485003152
230-234	20.62760515481859	25.772666451739905	29.39638865985558	24.203339733585917
235-239	21.49303722892838	25.685706704858962	26.06110576964226	26.760150296570394
240-244	21.766526774072986	30.207385301862733	23.383270519561663	24.64281740450262
245-249	20.147215319522864	28.575121181602647	24.38781536460042	26.889848134274065
250-254	20.995301092417556	25.375128028826815	25.85625964674029	27.773311232015335
255-259	21.116097538708214	25.744592832668108	27.994077151098267	25.145232477525415
260-264	20.195088828468474	26.989189944851315	27.26079650335086	25.554924723329346
265-269	19.78568188367137	26.64637225504769	27.75396782375245	25.813978037528496
270-274	18.811778366765854	28.682508480572427	25.339978944785695	27.165734207876017
275-279	20.31948006744516	27.15243918095787	23.634164224965552	28.893916526631426
280-284	17.364503419332568	28.2052569563518	27.703868966953582	26.726370657362047
285-289	19.918061550839212	25.99428827384332	28.731865554753544	25.355784620563927
290-294	19.08001837338484	29.22817518623023	25.410847714881584	26.280958725503346
295-299	18.49503718898624	30.060683524152154	23.038569843112978	28.405709443748627
300-301	13.96434308016673	29.363464923667713	31.187964862328	25.484227133837557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.25
5	0.25
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.25
22	0.25
23	0.25
24	0.0
25	0.0
26	0.75
27	0.75
28	0.75
29	0.5
30	1.75
31	3.25
32	4.5
33	12.0
34	39.75
35	123.25
36	472.5
37	1443.0
38	2898.0
39	4303.75
40	5447.0
41	6907.5
42	9129.25
43	11389.5
44	13008.25
45	12394.25
46	10061.5
47	8042.75
48	6823.0
49	6093.5
50	5398.75
51	4241.25
52	2652.75
53	1348.0
54	594.0
55	282.0
56	178.25
57	171.5
58	199.25
59	257.25
60	382.75
61	501.5
62	564.5
63	489.75
64	343.25
65	201.75
66	127.0
67	64.25
68	40.25
69	23.0
70	14.25
71	11.5
72	6.5
73	4.0
74	4.5
75	3.5
76	2.5
77	2.75
78	0.75
79	1.0
80	1.25
81	1.0
82	1.75
83	1.0
84	0.75
85	1.0
86	1.0
87	0.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.25
93	0.5
94	1.0
95	0.5
96	0.25
97	0.0
98	0.25
99	1.0
100	1.9999999999999998
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	350507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.201197586935944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.522463591964744	7.458818832221405
2	5.738711723233782	1.6299315815221718
3	5.363732661101515	2.2851428197141295
4	4.706774628883714	2.673673460078189
5	4.231123750779536	3.0043512199798847
6	3.629417724055078	3.0925246934900104
7	3.2478075775621313	3.2285929993315325
8	2.8753738745155455	3.266700202264705
9	2.4038417530906813	3.072368853303052
>10	14.688069621013828	36.53378655281806
>50	0.49852458913800707	4.548043830705126
>100	0.04403363048482976	1.0038250928699615
>500	0.00835414569610623	0.9330947416540675
>1k	0.029239509936369753	9.934819283675411
>5k	0.008354145696105644	8.554752585354965
>10k+	0.004177072848052822	8.779573251017311
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	18776	5.356811704188504	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10825	3.0883833989050147	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	9269	2.644455032281809	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	7912	2.2573015660172264	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6491	1.8518888353156997	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5171	1.4752915063037257	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4597	1.3115287283848824	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4471	1.2755808015246486	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3138	0.8952745594239203	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2876	0.82052569563518	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2851	0.8133931704644985	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2723	0.7768746415906103	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2333	0.6656072489279814	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2182	0.6225267968970662	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1784	0.5089769961798195	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1542	0.4399341525276243	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1433	0.4088363427834537	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1408	0.4017038176127723	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1093	0.3118340004621877	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1065	0.30384557227102454	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	903	0.2576268091650096	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	888	0.25334729406260076	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	714	0.2037049188746587	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	641	0.18287794537626922	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	409	0.1166881117923465	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	364	0.10384956648512012	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.853010068272531E-4	0.0	0.0
2	2.853010068272531E-4	0.0	0.0
3	2.853010068272531E-4	0.0	0.0
4	2.853010068272531E-4	0.0	0.0
5	2.853010068272531E-4	0.0	0.0
6	5.706020136545062E-4	0.0	0.0
7	5.706020136545062E-4	0.0	0.0
8	8.559030204817593E-4	0.0	0.0
9	0.0011412040273090123	0.0	0.0
10-14	0.0023965284573489254	0.0	0.0
15-19	0.002681829464176179	0.0	0.0
20-24	0.003309491679196136	0.0	0.0
25-29	0.00370891308875429	0.0	0.0
30-34	0.006561923157026821	0.0	0.0
35-39	0.007018404767950426	0.0	0.0
40-44	0.00736076597614313	0.0	0.0
45-49	0.008444909802086691	0.0	0.0
50-54	0.01192558208537918	0.0	0.0
55-59	0.015577434972768017	0.0	0.0
60-64	0.019172227658791407	0.0	0.0
65-69	0.023965284573489263	0.0	0.0
70-74	0.031611351556459645	0.0	0.0
75-79	0.03908623793533368	0.0	0.0
80-84	0.05112594042344375	0.0	0.0
85-89	0.06727397740986626	0.0	0.0
90-94	0.08536206124271413	0.0	0.0
95-99	0.10847144279572163	0.0	0.0
100-104	0.14013985455354672	0.0	0.0
105-109	0.1776854670520132	0.0	0.0
110-114	0.21842645082694498	0.0	0.0
115-119	0.266927621987578	0.0	0.0
120-124	0.326441412011743	0.0	0.0
125-129	0.39377244962297475	0.0	0.0
130-134	0.461046427032841	0.0	0.0
135-139	0.5445255016304953	0.0	0.0
140-144	0.6359359442179471	0.0	0.0
145-149	0.7454915308396124	0.0	0.0
150-154	0.8772435357924377	0.0	0.0
155-159	1.0252006379330514	0.0	0.0
160-164	1.187879272025951	0.0	0.0
165-169	1.37452319069234	0.0	0.0
170-174	1.5778857483588062	0.0	0.0
175-179	1.81012076791619	0.0	0.0
180-184	2.068888781108509	0.0	0.0
185-189	2.3466578413555217	0.0	0.0
190-194	2.6307035237527354	0.0	0.0
195-199	2.9313537247472947	0.0	0.0
200-204	3.2169970927827407	0.0	0.0
205-209	3.5259780831766556	0.0	0.0
210-214	3.8286253912190054	0.0	0.0
215-219	4.135609274565129	0.0	0.0
220-224	4.452692813552939	0.0	0.0
225-229	4.767722185291591	0.0	0.0
230-234	5.075561971658198	0.0	0.0
235-239	5.358295269424006	0.0	0.0
240-244	5.6049094597254845	0.0	0.0
245-249	5.801881274839019	0.0	0.0
250-254	5.972719517727178	0.0	0.0
255-259	6.123757870741526	0.0	0.0
260-264	6.2596752703940295	0.0	0.0
265-269	6.369116736612963	0.0	0.0
270-274	6.456304724299372	0.0	0.0
275-279	6.518899765197271	0.0	0.0
280-284	6.5627219998459365	0.0	0.0
285-289	6.595132194221513	0.0	2.2824080546180245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6135	0.0	292.83618	1
GGGTTAT	6245	0.0	287.67813	2
GTTATTA	6245	0.0	285.7886	4
GGTTATT	6310	0.0	283.7797	3
TTATTAG	6275	0.0	283.71713	5
GGGGGGA	5040	0.0	280.9524	1
TTAGTAG	6440	0.0	280.3416	8
GGGGAGC	5075	0.0	276.68967	3
TATTAGT	6525	0.0	273.75095	6
ATTAGTA	6540	0.0	273.3486	7
GGAGCGA	3690	0.0	270.61652	5
TAGTAGA	6710	0.0	268.40164	9
GGGAGCG	3755	0.0	268.28894	4
CGAAAGC	3795	0.0	266.23846	9
GAGCGAA	3820	0.0	261.02094	6
GCGAAAG	3965	0.0	254.82346	8
GGGGGAG	5725	0.0	247.59389	2
AGCGAAA	4080	0.0	244.02573	7
GGGAGCA	1905	0.0	211.37796	4
GCAAAAG	2365	0.0	167.76955	8
>>END_MODULE