##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30337_flu.3520000003ba83.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426457
Sequences flagged as poor quality	0
Sequence length	301
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.10065493121229	34.0	34.0	34.0	34.0	34.0
2	33.34453883978924	34.0	34.0	34.0	34.0	34.0
3	33.370536771585414	34.0	34.0	34.0	34.0	34.0
4	33.49345186032824	34.0	34.0	34.0	34.0	34.0
5	33.399559627348125	34.0	34.0	34.0	34.0	34.0
6	37.00850730554311	38.0	38.0	38.0	36.0	38.0
7	36.114396058688214	38.0	38.0	38.0	34.0	38.0
8	36.85925192926837	38.0	38.0	38.0	35.0	38.0
9	36.95737671089934	38.0	38.0	38.0	36.0	38.0
10-14	37.00966521829868	38.0	38.0	38.0	36.8	38.0
15-19	36.82734859552077	38.0	38.0	38.0	35.6	38.0
20-24	36.89559228714735	38.0	38.0	38.0	36.0	38.0
25-29	36.866413729871944	38.0	38.0	38.0	36.0	38.0
30-34	36.510638118262804	38.0	37.6	38.0	34.4	38.0
35-39	36.79102981074293	38.0	38.0	38.0	35.6	38.0
40-44	36.27396478425726	38.0	37.2	38.0	33.4	38.0
45-49	36.606326546404446	38.0	37.8	38.0	34.4	38.0
50-54	36.559525110386275	38.0	37.6	38.0	34.4	38.0
55-59	36.22760184496913	38.0	37.0	38.0	33.4	38.0
60-64	36.40831596151546	38.0	37.0	38.0	34.0	38.0
65-69	36.292403220019835	38.0	37.0	38.0	33.6	38.0
70-74	36.12997699650844	38.0	37.0	38.0	32.8	38.0
75-79	36.16448926855463	38.0	37.0	38.0	32.8	38.0
80-84	35.44552252630395	38.0	36.6	38.0	29.2	38.0
85-89	35.642175412761425	38.0	37.0	38.0	30.4	38.0
90-94	35.7195506229233	38.0	37.0	38.0	31.2	38.0
95-99	34.81593876991115	38.0	35.8	38.0	25.8	38.0
100-104	35.14780481971219	38.0	36.0	38.0	27.6	38.0
105-109	34.98426476760845	38.0	35.8	38.0	26.8	38.0
110-114	34.55287871930816	38.0	35.0	38.0	24.0	38.0
115-119	34.53498523884002	38.0	35.0	38.0	24.0	38.0
120-124	33.94916439406552	37.4	34.2	38.0	23.4	38.0
125-129	33.49059295544451	37.0	33.0	38.0	23.0	38.0
130-134	33.475331862297956	37.0	33.2	38.0	23.0	38.0
135-139	33.02318592495844	37.0	31.4	38.0	21.4	38.0
140-144	31.650952851049464	36.2	27.6	38.0	16.8	38.0
145-149	31.45578710163041	35.2	28.0	37.6	17.4	38.0
150-154	30.63743824113568	35.0	26.0	37.0	13.2	38.0
155-159	30.281154254707975	35.0	24.8	37.0	11.6	37.4
160-164	29.733528116551025	35.0	24.4	37.0	10.2	37.4
165-169	28.494366841205554	33.2	22.4	36.6	9.8	37.0
170-174	28.087787983313678	33.0	22.0	36.2	9.6	37.0
175-179	27.364444715410933	30.8	20.6	36.2	9.2	37.0
180-184	26.185931524163045	28.2	20.0	35.0	9.0	37.0
185-189	25.632134541114347	27.6	18.4	35.0	9.0	36.8
190-194	24.628283742557862	25.6	16.0	35.0	8.8	36.0
195-199	23.884469477579216	25.0	15.0	35.0	8.0	36.0
200-204	22.92230729006676	23.8	14.0	33.6	8.0	36.0
205-209	22.30086409649742	23.0	11.2	32.6	8.0	36.0
210-214	21.056870446492848	21.2	9.0	29.6	8.0	35.2
215-219	20.557735480951187	20.4	9.0	28.4	8.0	35.0
220-224	19.957673106550015	20.0	9.0	28.0	8.0	35.0
225-229	19.22945806962953	18.8	9.0	26.2	8.0	34.0
230-234	18.68119833887121	17.6	9.0	25.8	8.0	33.6
235-239	17.631254733771517	15.8	9.0	25.0	8.0	31.8
240-244	16.939277816989755	15.0	9.0	24.0	8.0	31.0
245-249	16.184752976267244	14.2	8.6	22.8	7.4	29.4
250-254	15.266997141564095	13.0	8.0	21.0	7.0	28.0
255-259	14.582758402371166	12.2	8.0	19.8	7.0	26.2
260-264	14.197665884250933	11.8	8.0	19.2	7.0	25.4
265-269	13.598521304609843	11.0	8.0	17.8	7.0	24.6
270-274	13.042831047444409	9.0	8.0	16.2	7.0	23.6
275-279	12.548164996705411	9.0	8.0	15.6	7.0	22.6
280-284	12.164932924069717	9.0	8.0	14.8	7.0	21.6
285-289	11.773401773215118	9.0	7.8	14.0	7.0	21.0
290-294	11.674814107870194	8.8	7.0	14.0	7.0	20.6
295-299	11.206681564612612	8.0	7.0	13.0	7.0	19.4
300-301	10.927697282492725	8.0	7.0	13.0	7.0	17.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.13902373063972817
1101	2	-0.13874574005409102
1101	3	0.03384941640357653
1101	4	-0.04153715615183273
1101	5	0.01167838620354189
1101	6	0.03428508211056425
1101	7	1.054806500542675
1101	8	0.09586973344184457
1101	9	0.16619172073129107
1101	10-14	0.05435516799087736
1101	15-19	0.1967205583034044
1101	20-24	0.24122313593908018
1101	25-29	0.11845502765775251
1101	30-34	0.412865302168008
1101	35-39	0.22340143650468747
1101	40-44	0.4323704630371523
1101	45-49	0.1474317186217533
1101	50-54	0.15963106889999779
1101	55-59	0.2182365847232859
1101	60-64	0.07494324952194376
1101	65-69	0.18049043879481985
1101	70-74	0.10237171699675685
1101	75-79	0.24714162238323212
1101	80-84	0.6855410816880081
1101	85-89	0.2621768705780596
1101	90-94	0.20251653742217712
1101	95-99	0.896081463510555
1101	100-104	0.27016376237656914
1101	105-109	0.40554928899056364
1101	110-114	0.3556384802131518
1101	115-119	0.3302843697426141
1101	120-124	0.6755738624057983
1101	125-129	0.557634363535513
1101	130-134	0.4126022367031581
1101	135-139	0.47318716092289037
1101	140-144	0.908932926738391
1101	145-149	1.134589650898537
1101	150-154	0.8947646669873102
1101	155-159	0.627865516338197
1101	160-164	0.9375095459531053
1101	165-169	0.5716260666075534
1101	170-174	0.3424596729082161
1101	175-179	0.4235780091512602
1101	180-184	0.26767183813898754
1101	185-189	0.6211619503262291
1101	190-194	0.6684012506009651
1101	195-199	0.9017811045109632
1101	200-204	0.9050947454235541
1101	205-209	0.3818422346005512
1101	210-214	0.9185986978267451
1101	215-219	0.7770849068339274
1101	220-224	0.49044169535408955
1101	225-229	0.36293985598203804
1101	230-234	0.17760889942642066
1101	235-239	0.27538867600076244
1101	240-244	0.07132960254627463
1101	245-249	0.018490883442535022
1101	250-254	0.11343799355516637
1101	255-259	0.10082230061579622
1101	260-264	-0.0866967482650729
1101	265-269	-0.10464057213492417
1101	270-274	0.16226001753889285
1101	275-279	0.27253705831214425
1101	280-284	0.0930096285015054
1101	285-289	0.08996905528353594
1101	290-294	-2.1659441801702428E-4
1101	295-299	0.0729335286913404
1101	300-301	-0.11139947701024333
1102	1	-0.04700697840578272
1102	2	0.044233640830455556
1102	3	0.035088715115819014
1102	4	-0.07562676909748944
1102	5	0.01609278115962809
1102	6	-0.15258854420527967
1102	7	0.861903022659547
1102	8	0.08457609811438971
1102	9	0.07918842105802071
1102	10-14	-0.029947237073749022
1102	15-19	0.10789196253723787
1102	20-24	0.13471592786390119
1102	25-29	-0.08470194829159539
1102	30-34	0.3239584008435301
1102	35-39	0.06193375736656748
1102	40-44	0.40441230473859235
1102	45-49	0.11130923513832869
1102	50-54	0.014494628745104876
1102	55-59	0.27535401798357384
1102	60-64	0.09193329064044775
1102	65-69	0.09801462412983852
1102	70-74	0.14627783769248026
1102	75-79	0.29316539070880054
1102	80-84	0.7405489567356369
1102	85-89	0.35276539994628564
1102	90-94	0.388169632473641
1102	95-99	0.7840388290462315
1102	100-104	0.519886982967698
1102	105-109	0.7165152220279865
1102	110-114	0.6495985168743914
1102	115-119	0.5164454801930916
1102	120-124	0.9484729396459812
1102	125-129	0.972100182916634
1102	130-134	0.8887799547271058
1102	135-139	0.9598795033996907
1102	140-144	1.7367563153467138
1102	145-149	1.6047630799135
1102	150-154	1.4624783469198128
1102	155-159	1.0391303533404894
1102	160-164	1.3447057296896574
1102	165-169	1.1827219965790832
1102	170-174	1.2489953557318714
1102	175-179	1.0130341010082127
1102	180-184	1.2037203034552064
1102	185-189	1.264965820788774
1102	190-194	1.4482574692999748
1102	195-199	1.6619339297803606
1102	200-204	1.2586202347726214
1102	205-209	1.058250662802525
1102	210-214	1.6477189009034738
1102	215-219	1.51694607625625
1102	220-224	1.16651813387492
1102	225-229	0.9769724061788949
1102	230-234	0.9715630308131189
1102	235-239	0.980695738142856
1102	240-244	0.7367047128656985
1102	245-249	0.450362054620264
1102	250-254	1.019180589918328
1102	255-259	0.6056100221748224
1102	260-264	0.6808771465385313
1102	265-269	0.38023779943303815
1102	270-274	0.483870005704075
1102	275-279	0.38569554698251274
1102	280-284	0.3195675086859122
1102	285-289	0.19309974062704427
1102	290-294	0.2103459193845474
1102	295-299	0.127748552033351
1102	300-301	-0.20582481694777677
1103	1	-0.22434603433682554
1103	2	0.07228698361629426
1103	3	0.18737384226957943
1103	4	0.09196838266152696
1103	5	-0.04438329298506005
1103	6	0.028447686672159023
1103	7	1.031399054044421
1103	8	0.20977069677411464
1103	9	0.2919017211479158
1103	10-14	0.05208038914993551
1103	15-19	0.21407026041569566
1103	20-24	0.2526997979302763
1103	25-29	0.07383205118787117
1103	30-34	0.4724599712048132
1103	35-39	0.16971498935372153
1103	40-44	0.4713291753845681
1103	45-49	0.2293052401775384
1103	50-54	0.21167276854956896
1103	55-59	0.31509429763082863
1103	60-64	0.23604934568852087
1103	65-69	0.35347176931361446
1103	70-74	0.41033899795555584
1103	75-79	0.3530762305926274
1103	80-84	0.8295372672440493
1103	85-89	0.49580415681411694
1103	90-94	0.41740166433113757
1103	95-99	1.1342402536281853
1103	100-104	0.5415651539079533
1103	105-109	0.7719062635439684
1103	110-114	0.5952114846895711
1103	115-119	0.6411296116403875
1103	120-124	0.9882545740032782
1103	125-129	1.0112495213211048
1103	130-134	0.9975536997553576
1103	135-139	1.0552088162249191
1103	140-144	1.6132829667807975
1103	145-149	1.5687566994725195
1103	150-154	1.8747943863201506
1103	155-159	0.9135345022238255
1103	160-164	0.7505943849270906
1103	165-169	1.4578454619441175
1103	170-174	1.8803522852005372
1103	175-179	1.9531515228374303
1103	180-184	1.4271989211540657
1103	185-189	1.3818819729166272
1103	190-194	1.3650774996693436
1103	195-199	1.1994961414749596
1103	200-204	1.4620743909209821
1103	205-209	1.2589912154866703
1103	210-214	1.7519153661136215
1103	215-219	1.7726524673951687
1103	220-224	1.7020961178407887
1103	225-229	1.0463498022372626
1103	230-234	1.8325304116868075
1103	235-239	1.3124762739657747
1103	240-244	0.5276852346375982
1103	245-249	1.0409229618052898
1103	250-254	0.9772836381915688
1103	255-259	0.8659918906093633
1103	260-264	0.554274731359591
1103	265-269	0.784594733691824
1103	270-274	0.5885667192021575
1103	275-279	0.4820384493806067
1103	280-284	0.5813852956820114
1103	285-289	0.4535304682943071
1103	290-294	0.2330806313146656
1103	295-299	0.20652192981900797
1103	300-301	-0.11066228016363944
2116	1	0.09579590865328669
2116	2	0.0063765705854592625
2116	3	0.038671108121818065
2116	4	0.05161934252191003
2116	5	-0.030545014916413038
2116	6	0.07186362617186859
2116	7	1.0184775880758323
2116	8	0.2423477764716182
2116	9	0.25043575256152906
2116	10-14	0.09300650898704532
2116	15-19	0.18072668857160323
2116	20-24	0.15786233428862317
2116	25-29	0.09734851424779833
2116	30-34	0.5207424610244757
2116	35-39	0.025756874170475896
2116	40-44	0.34019386415710784
2116	45-49	0.16612432103849528
2116	50-54	0.20370581101612117
2116	55-59	0.30189918419277717
2116	60-64	0.2604267744261577
2116	65-69	0.3667529793194291
2116	70-74	0.46396087113763684
2116	75-79	0.38339013402485733
2116	80-84	1.0173385470578893
2116	85-89	0.536368205679743
2116	90-94	0.5351180110443394
2116	95-99	1.111857472941736
2116	100-104	0.5330522975961429
2116	105-109	0.5987777004258774
2116	110-114	0.9071937999891659
2116	115-119	0.7245497396217715
2116	120-124	0.9487357840090951
2116	125-129	1.1005879739098248
2116	130-134	1.0920495694470418
2116	135-139	1.2655082926647054
2116	140-144	1.8202982082002244
2116	145-149	1.9698410508395945
2116	150-154	2.16476855734982
2116	155-159	1.9160871491115614
2116	160-164	1.8026134657880633
2116	165-169	1.8590545369877454
2116	170-174	1.8148774742639446
2116	175-179	2.2097500103301577
2116	180-184	2.070296536046449
2116	185-189	1.4612383428991755
2116	190-194	1.405933346091679
2116	195-199	0.5423210396715845
2116	200-204	0.9872285503159794
2116	205-209	1.0994910409665977
2116	210-214	1.932436599388783
2116	215-219	2.057824311490574
2116	220-224	1.313284017782614
2116	225-229	1.2927930832145762
2116	230-234	1.5432124361196138
2116	235-239	1.0367033827499483
2116	240-244	1.049966560990132
2116	245-249	1.079857225912324
2116	250-254	1.0959563548873206
2116	255-259	1.6504456427908618
2116	260-264	0.9649465172816392
2116	265-269	0.8784479041397582
2116	270-274	0.7153809135011713
2116	275-279	0.6670655581647846
2116	280-284	0.3051772677587987
2116	285-289	0.1474832315287422
2116	290-294	0.31044700711454887
2116	295-299	0.21739848595629496
2116	300-301	0.11825569540354941
1117	1	0.09014393192055792
1117	2	0.16546165389414824
1117	3	0.1453101695434853
1117	4	0.11717391217585771
1117	5	0.23143506987112517
1117	6	0.08502501826493614
1117	7	1.018207845396148
1117	8	0.32378324921143786
1117	9	0.30329585312349394
1117	10-14	0.12331864543582327
1117	15-19	0.14626619364899796
1117	20-24	0.26495522593803855
1117	25-29	0.19106974070938776
1117	30-34	0.6626504566865066
1117	35-39	0.33753918709563635
1117	40-44	0.575465006817069
1117	45-49	0.215653651611305
1117	50-54	0.2885775846531118
1117	55-59	0.3999700508002917
1117	60-64	0.45907159351321525
1117	65-69	0.519943475227933
1117	70-74	0.4751536149018136
1117	75-79	0.5609420220264312
1117	80-84	1.0759416427889334
1117	85-89	0.6389679099092618
1117	90-94	0.5951676216132071
1117	95-99	1.1888043328451232
1117	100-104	0.6755761268379814
1117	105-109	0.7159201632080894
1117	110-114	0.8060376134565814
1117	115-119	0.7794109838296066
1117	120-124	1.063214892754047
1117	125-129	1.0457874612409839
1117	130-134	0.9325538778370657
1117	135-139	1.0447702861577568
1117	140-144	1.4702378315858162
1117	145-149	1.8647628688421527
1117	150-154	2.2594226833480455
1117	155-159	1.893555748145367
1117	160-164	2.0635325627021928
1117	165-169	1.2830469652634058
1117	170-174	0.927594264168313
1117	175-179	1.3384218431212425
1117	180-184	1.9844140949540652
1117	185-189	1.4670572130559343
1117	190-194	-0.668349055564633
1117	195-199	1.3713507153099584
1117	200-204	1.6651660441540947
1117	205-209	1.7271571752464006
1117	210-214	2.1903334740806883
1117	215-219	1.7403098877500653
1117	220-224	1.763495592267482
1117	225-229	1.4591746271364947
1117	230-234	1.0354048052411748
1117	235-239	1.090342266709328
1117	240-244	1.0254654074852496
1117	245-249	1.4009440838439033
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2108	255-259	-0.635058966189213
2108	260-264	-0.539300563697644
2108	265-269	-0.2618000490766157
2108	270-274	-0.3683658998599171
2108	275-279	-0.2976018617975509
2108	280-284	-0.7970162499337352
2108	285-289	-1.091203338002428
2108	290-294	-1.009826240977434
2108	295-299	-0.7890898478726278
2108	300-301	2.664339549105602
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	85.0
12	317.0
13	533.0
14	742.0
15	1438.0
16	2702.0
17	3422.0
18	3736.0
19	4853.0
20	6087.0
21	8184.0
22	11063.0
23	14980.0
24	20331.0
25	28522.0
26	39412.0
27	52042.0
28	62232.0
29	66201.0
30	55148.0
31	31693.0
32	10907.0
33	1739.0
34	77.0
35	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.588446197389185	22.75024211116244	16.795597211442185	30.86571448000619
2	28.97689567764159	26.74103133492943	17.065260975901438	27.21681201152754
3	42.1343769711835	26.226325280157205	19.365610131853856	12.273687616805445
4	42.925312516853985	24.534712761192807	19.615576716996085	12.924398004957125
5	41.70244596758876	24.653130327324913	20.50570162994159	13.13872207514474
6	26.91080226142378	24.62522598995913	34.053374666144535	14.41059708247256
7	17.034777246006044	34.63772431921624	34.23533908459704	14.092159350180674
8	25.906246116255566	35.71286202360378	22.894688092820612	15.486203767320033
9	14.93632417805312	21.753189653353093	37.344914024157184	25.9655721444366
10-14	23.10840248841032	34.63340969898489	27.186093791402183	15.072094021202606
15-19	23.619872578009037	32.446366222151354	23.46557800669235	20.468183193147258
20-24	21.644573778833504	29.67159643293462	29.017556283517447	19.666273504714425
25-29	17.466192371094856	28.69780540593776	34.34644055555425	19.489561667413128
30-34	17.27452005712182	30.82177101091083	31.602013802094937	20.301695129872414
35-39	19.110719251882372	32.24831577392328	30.731163986052522	17.90980098814183
40-44	18.457101184879132	30.052877546857008	32.05847248374397	19.43154878451989
45-49	19.932373017678216	28.971314810168437	33.09454411581941	18.001768056333933
50-54	18.724232454854768	32.43042088651376	31.358425351207742	17.486921307423724
55-59	20.88257432754064	32.32461889475375	27.532811045427792	19.259995732277815
60-64	18.193627962490943	31.636530763945718	29.023371641220567	21.146469632342768
65-69	18.53415467444549	31.75560490272173	29.61499049142117	20.095249931411608
70-74	19.738637189681494	32.458278325833554	26.37541416836868	21.42767031611628
75-79	18.721465470141187	35.41632567879059	27.069692841247768	18.79251600982045
80-84	20.075271363818626	32.14237308802529	26.069169928034945	21.713185620121138
85-89	20.030155443573445	32.5559669556368	26.257653174880467	21.156224425909294
90-94	19.036292052891614	30.91974102899003	29.462571841944207	20.58139507617415
95-99	20.235756477206376	30.04846913053368	27.90635398176135	21.809420410498596
100-104	19.67490274517712	28.78325364573685	29.649319861087985	21.892523747998037
105-109	19.74234213531493	28.56677226543356	32.312097116473645	19.378788482777864
110-114	18.943387023779653	28.826118459774374	29.464307069645944	22.76618744680003
115-119	17.46398816293319	30.68839296810698	30.84207786482576	21.005541004134063
120-124	20.761718063016904	29.884935644156386	28.154819829431805	21.198526463394902
125-129	21.019610417931936	30.804653224123417	28.876017980710834	19.299718377233813
130-134	20.912354586746144	29.71868207111151	29.393819306518594	19.975144035623757
135-139	20.145899821083955	27.529715774392262	31.842272491716635	20.482111912807152
140-144	18.807757874768146	30.215519970360432	29.498917827588713	21.477804327282705
145-149	19.034556825189878	31.7875424720429	28.21030021784142	20.967600484925796
150-154	19.55385888846941	28.037809204679487	29.323331543391244	23.085000363459855
155-159	18.877307676975636	30.53658399322792	30.882269490241686	19.70383883955475
160-164	20.5400309996084	28.869592948409807	28.602274086250194	21.988101965731598
165-169	20.524460848338755	28.488968407131317	29.60495430957869	21.381616434951237
170-174	19.015469320470764	30.57016299415885	31.933582987264835	18.480784698105555
175-179	19.47333494349958	31.109396726985374	29.04203706352575	20.375231265989303
180-184	22.38007583414037	28.767542800329228	28.414916392508506	20.4374649730219
185-189	19.315007140227504	30.615138220266054	28.439631662746773	21.630222976759672
190-194	20.161798258675553	29.32403501408114	28.530989056340967	21.983177670902343
195-199	19.876751935130624	30.192024049317983	31.49227237447152	18.438951641079875
200-204	20.863674415005498	28.10018360585006	28.882724401287817	22.15341757785662
205-209	21.953913290202763	27.89219077187149	29.797517686425596	20.35637825150015
210-214	21.61779499457155	27.541065101522545	28.945661579010313	21.895478324895592
215-219	20.759185568533287	29.96499060866629	27.94710838372919	21.32871543907123
220-224	21.349303681262118	28.126962390112016	30.002649739598596	20.521084189027263
225-229	19.978192408613296	30.477867639644796	28.83667052012278	20.70726943161913
230-234	18.702846945881998	30.27221970796587	27.2494999495846	23.775433396567532
235-239	21.084845599908082	29.555852055424108	27.140274400467106	22.219027944200707
240-244	19.099369924752086	29.82045083091613	28.85355381668023	22.226625427651555
245-249	21.50397343694675	29.021401923288863	28.042733499508742	21.43189114025564
250-254	21.51893391361849	30.357621049718965	26.815036451506245	21.3084085851563
255-259	20.215871705705382	30.0298037082285	26.754350379991415	22.999974206074704
260-264	19.105044588317227	31.75790290697538	27.25508081705776	21.881971687649635
265-269	20.25639161744326	31.35134374626281	26.663977845363075	21.728286790930856
270-274	19.614920144352187	30.425904604684646	27.715666526754163	22.243508724209004
275-279	19.367720543923536	32.19124085194991	25.3851619272283	23.055876676898258
280-284	19.674855847131127	30.50244221574508	27.57206470992386	22.250637227199928
285-289	19.73666747174979	32.62467259301641	25.290849956736555	22.347809978497246
290-294	18.315984964486454	33.1727700565356	24.92579556672771	23.585449412250238
295-299	19.34520948184694	31.992486933031934	25.02850228745219	23.63380129766893
300-301	14.578726577357154	33.18259519717111	35.92659986821649	16.312078357255245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.25
13	0.25
14	0.25
15	0.0
16	0.5
17	0.25
18	0.0
19	1.25
20	0.25
21	1.25
22	0.75
23	1.25
24	1.5
25	3.25
26	4.25
27	5.75
28	10.75
29	21.0
30	77.25
31	283.25
32	807.75
33	1903.0
34	3633.5
35	5743.75
36	7497.0
37	8287.75
38	9245.5
39	10894.0
40	12683.75
41	13765.0
42	13319.75
43	12214.25
44	11179.0
45	9806.25
46	7707.75
47	5350.25
48	3331.75
49	1967.5
50	1103.5
51	564.75
52	265.25
53	127.25
54	75.25
55	53.0
56	37.5
57	26.75
58	27.0
59	22.0
60	15.5
61	16.75
62	13.75
63	10.5
64	12.75
65	11.0
66	8.25
67	5.5
68	5.5
69	4.5
70	3.0
71	2.0
72	2.25
73	1.25
74	0.75
75	1.25
76	0.5
77	1.25
78	1.5
79	1.0
80	0.75
81	0.75
82	1.5
83	1.0
84	0.5
85	1.0
86	0.25
87	0.75
88	1.0
89	0.25
90	0.0
91	0.75
92	0.75
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.25
99	0.5
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	426457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.74652217435285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.98485519340475	7.960888641887706
2	5.5855303812473105	1.6473429524516998
3	3.5130063797396005	1.5541387943241913
4	3.1649556955537705	1.866883573813121
5	3.2964219920017572	2.430538000053915
6	2.866447535570932	2.53620792869495
7	2.665119698097099	2.751087270770541
8	2.4728379972209282	2.9172608287680566
9	2.360812920981388	3.1332402079875683
>10	19.76673243117311	49.588291109810626
>50	0.18584673320778267	1.7202394497406432
>100	0.07250418985726259	2.0190991143660675
>500	0.011362549450667492	1.1496878394293393
>1k	0.05194308120590764	16.759637997899944
>5k	0.0016232212876846122	1.9654562900016623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	8211	1.9253992782390723	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	4843	1.1356361837184052	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	4027	0.9442921560673174	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	3686	0.8643309876493995	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	3574	0.8380680818933679	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	3543	0.8307988847644663	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA	3137	0.7355958513988515	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	3067	0.7191815353013317	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	2755	0.6460205835523863	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	2677	0.62773034561515	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT	2604	0.6106125588277365	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	2338	0.5482381576571612	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2238	0.5247891346607043	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	2186	0.5125956427025468	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2181	0.511423191552724	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2138	0.5013401116642475	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2068	0.4849257955667277	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1893	0.4438900053229282	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1795	0.42090996278640047	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	1721	0.40355768576902246	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1625	0.38104662369242387	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1603	0.37588783863320335	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1577	0.3697910926541246	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1568	0.3676806805844435	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	1489	0.34915595241724257	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1395	0.32711387080057314	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA	1291	0.302726886884258	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1280	0.3001474943546477	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1240	0.29076788515606494	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	1197	0.28068480526758854	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1129	0.26473946962999784	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT	1104	0.25887721388088364	Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	1047	0.24551127077290327	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	908	0.2129171288078282	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	847	0.19861322477998955	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	758	0.17774359431314293	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTT	653	0.15312212016686325	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	556	0.1303765678603001	No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	542	0.12709370464079614	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	539	0.12639023395090243	No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC	441	0.10341019141437471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	7.034706898937056E-4	0.0	0.0
2	7.034706898937056E-4	0.0	0.0
3	7.034706898937056E-4	0.0	0.0
4	7.034706898937056E-4	0.0	0.0
5	7.034706898937056E-4	0.0	0.0
6	9.379609198582741E-4	0.0	0.0
7	0.0011724511498228426	0.0	0.0
8	0.0016414316097519796	0.0	0.0
9	0.0021104120696811167	0.0	0.0
10-14	0.0026731886215960814	0.0	0.0
15-19	0.003470455403475614	0.0	0.0
20-24	0.004877396783263026	0.0	0.0
25-29	0.005768459657128385	0.0	0.0
30-34	0.01036446816443393	0.0	0.0
35-39	0.011865205636207167	0.0	0.0
40-44	0.05829427116919174	0.0	0.0
45-49	0.13534776073554894	0.0	0.0
50-54	0.14083483211671985	0.0	0.0
55-59	0.15138689246512543	0.0	0.0
60-64	0.17235031902395787	0.0	0.0
65-69	0.20213057822945807	0.0	0.0
70-74	0.23116046869907164	0.0	0.0
75-79	0.26783474066553015	0.0	0.0
80-84	0.3110747390709966	0.0	0.0
85-89	0.36599235092869853	0.0	0.0
90-94	0.43929399681562276	0.0	0.0
95-99	0.5252112170746406	0.0	0.0
100-104	0.6230405410158586	0.0	0.0
105-109	0.7316095174894538	0.0	0.0
110-114	0.8551858686807815	0.0	0.0
115-119	0.9740724152728177	0.0	0.0
120-124	1.1090449916404235	0.0	0.0
125-129	1.2454714074338091	0.0	0.0
130-134	1.4001411631184388	0.0	0.0
135-139	1.5631118729438138	0.0	0.0
140-144	1.7950227103787721	0.0	0.0
145-149	2.1385508972768648	0.0	0.0
150-154	2.45314298979733	0.0	0.0
155-159	3.4504299378366405	0.0	0.0
160-164	3.728535350574618	0.0	0.0
165-169	4.069577941035087	0.0	0.0
170-174	4.410667429541549	0.0	0.0
175-179	4.804892404157981	0.0	0.0
180-184	5.243576726375695	0.0	0.0
185-189	5.7372724565430975	0.0	0.0
190-194	6.25155642890139	0.0	0.0
195-199	6.81555233001217	0.0	0.0
200-204	7.39146033480515	0.0	0.0
205-209	7.963288209596747	0.0	0.0
210-214	8.56869508531927	0.0	0.0
215-219	9.1740550629958	0.0	0.0
220-224	9.777492220786622	0.0	0.0
225-229	10.322775801546229	0.0	0.0
230-234	10.8749064970208	0.0	0.0
235-239	11.368977411556148	0.0	0.0
240-244	11.799360779633115	0.0	0.0
245-249	12.152596862051743	0.0	0.0
250-254	12.472160147447457	0.0	0.0
255-259	12.739807295929015	0.0	0.0
260-264	12.971061560720074	0.0	0.0
265-269	13.14519400549176	0.0	0.0
270-274	13.278853436571566	1.4069413797874114E-4	4.6898045992913705E-5
275-279	13.36810041809608	2.3449022996456853E-4	2.3449022996456853E-4
280-284	13.441589656166977	2.3449022996456853E-4	2.3449022996456853E-4
285-289	13.498805272278329	2.3449022996456853E-4	3.7518436794330964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	5495	0.0	285.06824	1
CGGGTTA	5565	0.0	282.80774	2
TTATTAG	6090	0.0	257.70114	6
GGGGGGA	4935	0.0	254.35156	1
TATTAGT	6175	0.0	253.6761	7
ATTAGTA	6270	0.0	253.126	8
GTTATTA	6320	0.0	248.32278	5
GGGTTAT	6475	0.0	243.0618	3
GGGGAGC	5205	0.0	240.30739	3
GGAGCAG	5300	0.0	235.4434	5
GGGAGCA	5365	0.0	233.69057	4
CAGAAGC	5505	0.0	230.96275	9
GAGCAGA	5425	0.0	229.20276	6
TTAGTAG	6970	0.0	227.70444	9
GGTTATT	7260	0.0	217.18665	4
GGGGGAG	5825	0.0	215.99571	2
GCAGAAG	6325	0.0	201.25294	8
AGCAGAA	7355	0.0	169.6601	7
CCGAGAA	30	1.1485748E-4	147.5	295
CGGGTAA	160	0.0	110.625	2
>>END_MODULE