##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30304_flu.3520000003baad.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384180
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.982851788224266	34.0	34.0	34.0	33.0	34.0
2	33.25470612733614	34.0	34.0	34.0	34.0	34.0
3	33.268532979332605	34.0	34.0	34.0	34.0	34.0
4	33.343948149305014	34.0	34.0	34.0	34.0	34.0
5	33.25543495236608	34.0	34.0	34.0	34.0	34.0
6	36.70428965589047	38.0	38.0	38.0	35.0	38.0
7	35.98223488989536	38.0	38.0	38.0	34.0	38.0
8	36.637628195116875	38.0	38.0	38.0	34.0	38.0
9	36.72189598625644	38.0	38.0	38.0	35.0	38.0
10-14	36.6866395960227	38.0	38.0	38.0	35.4	38.0
15-19	36.589682961111976	38.0	38.0	38.0	35.0	38.0
20-24	36.679212348378364	38.0	38.0	38.0	35.0	38.0
25-29	36.60834244364621	38.0	38.0	38.0	34.6	38.0
30-34	36.26589723567078	38.0	37.4	38.0	32.6	38.0
35-39	36.53086469883908	38.0	38.0	38.0	34.4	38.0
40-44	36.0156197615701	38.0	37.0	38.0	32.0	38.0
45-49	36.33097037846842	38.0	37.0	38.0	34.0	38.0
50-54	36.260833463480665	38.0	37.0	38.0	33.8	38.0
55-59	35.901319693893484	38.0	37.0	38.0	32.4	38.0
60-64	36.04686813472851	38.0	37.0	38.0	32.8	38.0
65-69	35.96277057629236	38.0	37.0	38.0	32.2	38.0
70-74	35.83711489406008	38.0	37.0	38.0	31.6	38.0
75-79	35.81979072309855	38.0	37.0	38.0	31.6	38.0
80-84	35.01280545577593	38.0	36.2	38.0	27.2	38.0
85-89	35.22717163829455	38.0	36.6	38.0	27.6	38.0
90-94	35.170058826591706	38.0	36.2	38.0	27.4	38.0
95-99	34.22334270394086	38.0	34.8	38.0	23.0	38.0
100-104	34.5831917330418	38.0	35.4	38.0	24.0	38.0
105-109	34.253512416054974	37.8	34.8	38.0	23.8	38.0
110-114	33.75852048518924	37.0	34.0	38.0	23.0	38.0
115-119	33.73443698266438	37.0	34.0	38.0	23.0	38.0
120-124	33.213407257014936	37.0	32.8	38.0	21.8	38.0
125-129	32.89703732625331	37.0	31.0	38.0	21.0	38.0
130-134	32.651679421104696	37.0	30.6	38.0	20.2	38.0
135-139	31.968400749648605	36.2	28.2	38.0	17.0	38.0
140-144	30.58407100838149	35.4	25.6	37.6	13.0	38.0
145-149	30.418167525639024	35.0	25.2	37.6	11.2	38.0
150-154	29.432646676037272	34.0	23.2	37.0	10.0	38.0
155-159	29.105889947420486	34.0	23.0	37.0	10.0	37.0
160-164	28.425553126138787	32.8	21.4	37.0	10.0	37.2
165-169	27.04215055442761	30.0	20.0	35.8	9.4	37.0
170-174	26.501130719975016	29.2	20.0	35.4	9.0	37.0
175-179	26.040285803529592	28.4	18.8	35.4	9.0	37.0
180-184	24.85986204383362	25.8	16.6	35.0	8.6	36.6
185-189	24.296773387474623	25.2	15.4	35.0	8.4	36.2
190-194	23.335163204747776	24.0	14.6	34.0	8.0	36.0
195-199	22.536742672705504	23.0	13.0	32.8	8.0	36.0
200-204	21.68963975219949	22.4	10.6	31.0	8.0	35.4
205-209	20.841882971523766	20.8	9.0	29.0	8.0	35.0
210-214	19.620953199021294	19.2	9.0	27.4	8.0	34.4
215-219	19.327201311885055	18.8	9.0	26.6	8.0	34.0
220-224	18.676151283252644	18.0	9.0	26.0	8.0	33.2
225-229	17.86923265136134	16.2	9.0	25.2	8.0	32.2
230-234	17.23040397730231	15.6	9.0	24.2	8.0	31.2
235-239	16.414249049924514	14.8	9.0	22.8	8.0	29.2
240-244	15.631011505023688	13.6	8.0	21.6	7.0	28.6
245-249	14.919952105783747	12.8	8.0	20.2	7.0	26.8
250-254	14.337063355718673	12.0	8.0	19.6	7.0	25.8
255-259	13.727968139934404	11.4	8.0	18.4	7.0	24.6
260-264	13.333089697537613	10.6	8.0	17.6	7.0	23.8
265-269	12.844880524754021	9.8	8.0	16.4	7.0	22.8
270-274	12.336894164193868	9.0	7.6	15.2	7.0	22.0
275-279	11.984683221406632	9.0	7.2	14.2	7.0	21.0
280-284	11.893817481388933	8.6	8.0	14.0	7.0	21.0
285-289	11.495886823884637	8.0	7.6	13.4	7.0	19.8
290-294	11.147397053464521	8.0	7.4	13.0	7.0	19.0
295-299	10.924575980009369	8.0	7.0	13.0	7.0	19.0
300-301	10.66227809880785	8.0	7.0	13.0	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.1618936809574265
1101	2	-0.025764878063412766
1101	3	0.22507328248264713
1101	4	0.05418376887149634
1101	5	0.10776942944283263
1101	6	0.07247814809110764
1101	7	1.0334473526227512
1101	8	0.1957723652211314
1101	9	0.1937927618870816
1101	10-14	0.12112050440431688
1101	15-19	0.11278498831090644
1101	20-24	0.042734827264673925
1101	25-29	-0.047541144266020297
1101	30-34	0.32743177607126484
1101	35-39	0.1452856310282442
1101	40-44	0.3088899994330845
1101	45-49	0.07859147762828655
1101	50-54	0.0939551245238377
1101	55-59	0.30896481010820764
1101	60-64	0.2031931098620703
1101	65-69	0.17408641498354882
1101	70-74	0.1298889844973985
1101	75-79	0.22543975216979106
1101	80-84	0.6000538965572559
1101	85-89	0.3241432802104711
1101	90-94	0.28862938680799033
1101	95-99	0.9674484986735905
1101	100-104	0.428997392025785
1101	105-109	0.40451196396538336
1101	110-114	0.3561725528569184
1101	115-119	0.3516114504717862
1101	120-124	0.6458264191931633
1101	125-129	0.3717317554844115
1101	130-134	0.5914044484216276
1101	135-139	0.28430278908218654
1101	140-144	0.9555047911028254
1101	145-149	1.0875696632383196
1101	150-154	0.8033882190100528
1101	155-159	0.33292102844386307
1101	160-164	0.7694008647996462
1101	165-169	0.47072541924016775
1101	170-174	0.34668403805359205
1101	175-179	0.5196511491052789
1101	180-184	0.10327094251151436
1101	185-189	0.22360441293361788
1101	190-194	0.8382640160836594
1101	195-199	0.7142000015971668
1101	200-204	0.5487641623577169
1101	205-209	0.6126329599917995
1101	210-214	0.5570177127076903
1101	215-219	0.6673133999883625
1101	220-224	0.38027361106143687
1101	225-229	0.5648920962159814
1101	230-234	0.2281011516616651
1101	235-239	0.20386259948265106
1101	240-244	0.03903344859072888
1101	245-249	0.36707339327657884
1101	250-254	0.3377484546225755
1101	255-259	0.191404765408981
1101	260-264	0.19134929824507552
1101	265-269	0.02063182753635573
1101	270-274	0.039250254995774725
1101	275-279	0.024822961385918774
1101	280-284	0.013581743033524774
1101	285-289	0.10429007305541127
1101	290-294	0.028272789226152284
1101	295-299	-0.15003945081456038
1101	300-301	-0.3013049341120073
1102	1	-0.14659266595328546
1102	2	0.010994963532098723
1102	3	0.010080949674687645
1102	4	0.05195931779469021
1102	5	-0.10242951107215958
1102	6	-0.001662430306822671
1102	7	0.7973303560483416
1102	8	0.01777766366238609
1102	9	0.008925532858050644
1102	10-14	0.028073006429089276
1102	15-19	0.09096301146593788
1102	20-24	0.06535431402519265
1102	25-29	0.041335889013346616
1102	30-34	0.33529123222397317
1102	35-39	0.09556950512979512
1102	40-44	0.5288895319833316
1102	45-49	0.15244325337841502
1102	50-54	0.10128900457013401
1102	55-59	0.17472807932978185
1102	60-64	0.08095418816701283
1102	65-69	0.20496736467884347
1102	70-74	0.16350695407861338
1102	75-79	0.3304354689074387
1102	80-84	0.7045711886412036
1102	85-89	0.25538182896192296
1102	90-94	0.3113084226014351
1102	95-99	0.913871237868122
1102	100-104	0.5334732233495956
1102	105-109	0.8126801401898689
1102	110-114	0.9047164977059836
1102	115-119	0.6496065991563711
1102	120-124	0.7577168164543124
1102	125-129	0.7035942480280752
1102	130-134	0.9885521920112126
1102	135-139	0.9409194894357249
1102	140-144	1.570855637542536
1102	145-149	1.7451991036298367
1102	150-154	1.5616272748428557
1102	155-159	1.006562451808012
1102	160-164	1.1899377613194844
1102	165-169	1.0588513827201496
1102	170-174	1.3515560838474059
1102	175-179	1.0854120810781254
1102	180-184	1.2419892969258157
1102	185-189	1.2225374080772227
1102	190-194	1.2486926166915495
1102	195-199	1.0983337410020617
1102	200-204	1.3777015373022081
1102	205-209	1.2590560040448757
1102	210-214	1.7318660709936928
1102	215-219	1.3793440321735027
1102	220-224	1.0423519536065804
1102	225-229	0.9049680872856598
1102	230-234	1.0656505768509064
1102	235-239	0.7624914677563268
1102	240-244	0.8373272570156001
1102	245-249	0.3371283592696095
1102	250-254	0.6739978614186839
1102	255-259	0.44912932166198516
1102	260-264	0.4622321583871578
1102	265-269	0.41532952473663975
1102	270-274	0.41663259738645486
1102	275-279	0.3317937549225114
1102	280-284	0.3514306409191281
1102	285-289	0.14544516170319177
1102	290-294	-0.0557665456538281
1102	295-299	0.010569778472587998
1102	300-301	-0.32632111701899547
1103	1	0.05060873394740639
1103	2	0.19524948437844358
1103	3	0.13968688723975475
1103	4	0.09687546790760848
1103	5	0.09963810541271556
1103	6	0.14643633156590852
1103	7	0.9143397901617121
1103	8	0.38024222567286614
1103	9	0.14669522196476947
1103	10-14	0.12139384636950723
1103	15-19	0.2390665784897834
1103	20-24	0.2274378675946167
1103	25-29	0.2325396594552629
1103	30-34	0.6058887127230719
1103	35-39	0.33934823592921504
1103	40-44	0.6397962239571271
1103	45-49	0.23721913312149923
1103	50-54	0.28799375205944955
1103	55-59	0.46532840287662935
1103	60-64	0.3817057938883437
1103	65-69	0.42356790445748516
1103	70-74	0.34758188240177645
1103	75-79	0.44787956898271375
1103	80-84	0.9932832880117246
1103	85-89	0.6259979294216151
1103	90-94	0.5987861987774892
1103	95-99	1.2576399579360427
1103	100-104	0.9397943996505873
1103	105-109	1.0028155653656583
1103	110-114	1.042330419830499
1103	115-119	0.9952136899577084
1103	120-124	1.2479837826420734
1103	125-129	1.2029143191443197
1103	130-134	1.2753324204160634
1103	135-139	1.2767595099377012
1103	140-144	1.851144507210826
1103	145-149	2.2351581397165248
1103	150-154	1.9983170062349132
1103	155-159	0.935262418171952
1103	160-164	1.3263051471571004
1103	165-169	2.13407241967176
1103	170-174	1.934767886900282
1103	175-179	1.7236014200495013
1103	180-184	1.5595961714953575
1103	185-189	1.4040910335637413
1103	190-194	1.6155352720180574
1103	195-199	1.6977575232966835
1103	200-204	1.560096464664337
1103	205-209	1.2675543142333936
1103	210-214	1.8127117375482413
1103	215-219	1.9696272157462928
1103	220-224	1.8067644037531387
1103	225-229	1.253071854524137
1103	230-234	1.5533133513451638
1103	235-239	1.0598059889230171
1103	240-244	0.7729434375611444
1103	245-249	1.248091711983717
1103	250-254	0.9751753236582061
1103	255-259	1.1525776182359113
1103	260-264	0.8083762248263806
1103	265-269	0.7493117536860705
1103	270-274	0.5865773781914765
1103	275-279	0.5551201608296452
1103	280-284	0.4828655391300103
1103	285-289	0.5012293623663027
1103	290-294	0.28801719051133823
1103	295-299	0.27602394533947106
1103	300-301	0.0287375058246937
2116	1	0.07287857521724561
2116	2	0.058733611362569604
2116	3	0.03677418882706007
2116	4	0.057399277431422036
2116	5	0.17334445461906256
2116	6	0.19813474426432265
2116	7	0.8524191552410798
2116	8	0.3581469875776264
2116	9	0.19150474577429577
2116	10-14	0.15435575113140487
2116	15-19	0.29897451499771677
2116	20-24	0.09357913743588142
2116	25-29	0.02568886580446872
2116	30-34	0.45107125240561174
2116	35-39	0.026626013706987806
2116	40-44	0.4697216207825292
2116	45-49	0.09083659343895789
2116	50-54	0.15301756158324764
2116	55-59	0.3442649108131377
2116	60-64	0.26837880976135864
2116	65-69	0.4431758409654165
2116	70-74	0.35778505700495344
2116	75-79	0.4262621086652487
2116	80-84	0.793135668964112
2116	85-89	0.4692423738660594
2116	90-94	0.630967151158444
2116	95-99	1.2309812277773062
2116	100-104	0.5332229710791552
2116	105-109	0.6185806447307414
2116	110-114	0.7308478801479623
2116	115-119	0.599815277259296
2116	120-124	0.9081345762928592
2116	125-129	0.9550667000966939
2116	130-134	1.0678974997811466
2116	135-139	1.4425277639059466
2116	140-144	1.7543603802267
2116	145-149	2.0499819492403333
2116	150-154	2.289336053853958
2116	155-159	1.6923957515052876
2116	160-164	1.6127686392205938
2116	165-169	1.7503105149098594
2116	170-174	1.4915853472177432
2116	175-179	1.8147093565574401
2116	180-184	2.0321263302255197
2116	185-189	1.3815053192780233
2116	190-194	1.3214924148751983
2116	195-199	0.7075321264712855
2116	200-204	0.7410948773627482
2116	205-209	0.7874146316937143
2116	210-214	1.7498561819926834
2116	215-219	1.5713351522542247
2116	220-224	1.1982548799436152
2116	225-229	0.9586385211908031
2116	230-234	0.9621609703927874
2116	235-239	0.6990583698754023
2116	240-244	0.6736513740690793
2116	245-249	0.7258377437297518
2116	250-254	0.8205673871502679
2116	255-259	1.7356871420454372
2116	260-264	0.5344920978422554
2116	265-269	0.8173228647971822
2116	270-274	0.6450996004136993
2116	275-279	0.6049947640042479
2116	280-284	0.14045284071731245
2116	285-289	0.17614364808058802
2116	290-294	0.22136322225737182
2116	295-299	0.10968743740296105
2116	300-301	0.1287136963008848
1117	1	0.2484781826755409
1117	2	0.1051703140122271
1117	3	0.26211570011263063
1117	4	0.1941814167837279
1117	5	0.28146221713132746
1117	6	0.1216960789061261
1117	7	0.8341296557317577
1117	8	0.33719114851972876
1117	9	0.4191553836545694
1117	10-14	0.3561851917594794
1117	15-19	0.29623202235787716
1117	20-24	0.42349356334363364
1117	25-29	0.33302527404784144
1117	30-34	0.6718347460268106
1117	35-39	0.31907645531642004
1117	40-44	0.6650686276519977
1117	45-49	0.32232805565681844
1117	50-54	0.3765043133006287
1117	55-59	0.5892119176826185
1117	60-64	0.5872991237947218
1117	65-69	0.6158535642235208
1117	70-74	0.6028033101943535
1117	75-79	0.7288157887437663
1117	80-84	1.2135021066481073
1117	85-89	0.7917132276442658
1117	90-94	0.7997441874685549
1117	95-99	1.3323511983366814
1117	100-104	0.8443436776542299
1117	105-109	0.8651296143087492
1117	110-114	1.1217352206778912
1117	115-119	1.0365265628333944
1117	120-124	1.249276872661845
1117	125-129	1.3933367687914924
1117	130-134	1.21431477161628
1117	135-139	1.4144372830423642
1117	140-144	1.7629974754180608
1117	145-149	2.4074518216642815
1117	150-154	2.529792776130705
1117	155-159	2.191878381534732
1117	160-164	2.0714844390243172
1117	165-169	1.6840455492572524
1117	170-174	1.6925460930469853
1117	175-179	1.9980632329068015
1117	180-184	1.9335863891067717
1117	185-189	1.661235643124936
1117	190-194	-0.2040830512680749
1117	195-199	1.5675906151857113
1117	200-204	1.7825225515531322
1117	205-209	1.824146133165744
1117	210-214	2.372354906232136
1117	215-219	1.7300311287017252
1117	220-224	1.3271280889720742
1117	225-229	1.3658744387570465
1117	230-234	1.1423805974781622
1117	235-239	1.1263201951943387
1117	240-244	1.0639410698492515
1117	245-249	1.3627376455943239
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2108	260-264	-0.42668645342773637
2108	265-269	-0.38133395552266514
2108	270-274	-0.371762488015273
2108	275-279	-0.18541407889321349
2108	280-284	-0.9306224179844751
2108	285-289	-1.0722520245440599
2108	290-294	-0.7938303807529756
2108	295-299	-0.716672675485091
2108	300-301	2.549293658671532
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	36.0
11	328.0
12	653.0
13	766.0
14	1278.0
15	2236.0
16	3015.0
17	4305.0
18	5574.0
19	6138.0
20	7891.0
21	10030.0
22	13157.0
23	17050.0
24	22843.0
25	30263.0
26	39449.0
27	49497.0
28	55818.0
29	53561.0
30	37904.0
31	17438.0
32	4411.0
33	512.0
34	21.0
35	4.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.593888281534696	16.645843094382844	12.230204591597689	37.53006403248477
2	56.46207506897808	19.323494195429227	12.5862876776511	11.628143057941589
3	56.060700713207346	18.261752303607683	14.516632828361706	11.16091415482326
4	56.211151022957985	17.457962413452027	14.695455255349055	11.635431308240928
5	32.73751887136238	17.574574418241447	38.259409651725754	11.428497058670414
6	31.683585819147275	17.602686240824614	38.147222656046644	12.566505283981467
7	14.300848560570568	57.35696808787547	15.943828413764383	12.398354937789579
8	30.825654641053674	16.32411890259774	39.57441824144932	13.275808214899264
9	13.130303503566038	15.478421573220885	40.27409027018585	31.117184653027223
10-14	28.752563902337446	37.391899630381594	20.744078296631788	13.111458170649176
15-19	22.011296787963975	38.60305065333958	17.635639543963766	21.750013014732676
20-24	28.330209797490756	31.495809256077877	24.081368108699046	16.09261283773231
25-29	17.74709771461294	26.11447758863033	40.41464938310167	15.723775313655059
30-34	18.050288927065438	29.09651725753553	30.532042271851733	22.321151543547295
35-39	20.539174345358944	31.337810401374355	28.21224426050289	19.91077099276381
40-44	19.823572283825293	31.62335363631631	27.58753709198813	20.96553698787027
45-49	20.985163204747774	29.34228746941538	26.120464365661928	23.55208496017492
50-54	22.946847831745536	31.332656567234107	24.50793898693321	21.21255661408715
55-59	21.40272788796918	27.25029933885158	28.943411942318704	22.403560830860535
60-64	20.178093601957418	27.160549742308294	29.074236035191838	23.587120620542454
65-69	16.976209068665728	29.096309021812694	29.258004060596594	24.669477848924984
70-74	19.575927950439898	25.447498568379405	31.528918736009164	23.447654745171533
75-79	21.108907283044406	25.051798636056017	27.580613254203755	26.25868082669582
80-84	23.664792545161124	27.803789890155656	23.877661512832525	24.653756051850696
85-89	21.564683221406632	27.447602686240824	25.411473788328387	25.576240304024157
90-94	22.209276901452444	29.990785569264407	27.166380342547765	20.633557186735384
95-99	23.874433859128533	27.4779530428445	27.47540215523973	21.172210942787235
100-104	22.468686553178195	27.10817845801447	27.170961528450206	23.252173460357124
105-109	26.152168254464055	27.672913738351813	23.460929772502475	22.713988234681658
110-114	20.43411942318705	26.15857150294133	30.47399656411057	22.933312509761052
115-119	25.55749908896871	26.018324743609767	26.633192774220415	21.790983393201106
120-124	19.46020094747254	27.47477744807122	27.23827372585767	25.82674787859857
125-129	19.26216877505336	24.622000104117863	30.370399291998538	25.745431828830235
130-134	23.97974907595398	28.754906554219378	26.227133114685824	21.03821125514082
135-139	22.981571138528814	26.18970274350565	24.240616377739602	26.588109740225935
140-144	21.77073246915508	27.075381331667447	26.19391951689312	24.959966682284346
145-149	22.207923369253997	24.404445832682597	29.622937164870635	23.764693633192774
150-154	21.322088604300067	27.481076578687073	25.335832162007392	25.861002655005468
155-159	20.972408766723934	26.807642251028163	30.728772971003178	21.49117601124473
160-164	23.319329480972463	30.122807017543863	23.494872195325108	23.062991306158573
165-169	20.878806809308138	27.932739861523242	26.756728616794213	24.431724712374407
170-174	20.970899057733355	30.962725805611953	26.13071997501171	21.93565516164298
175-179	19.340777760424803	27.562444687386122	27.811234317247123	25.285543234941954
180-184	20.173148003540007	28.31277005570306	27.943307824457285	23.57077411629965
185-189	19.86766619813629	26.756103909625693	28.41444114737883	24.96178874485918
190-194	19.392055807173723	25.44458326825967	28.63662866364725	26.52673226091936
195-199	17.945546358477795	31.401894945077828	26.882711229111354	23.769847467333022
200-204	20.715185590087977	26.246915508355457	25.484980998490293	27.55291790306627
205-209	21.00848560570566	25.342339528346088	29.76396480816284	23.885210057785415
210-214	22.450726222083397	24.998334114217293	29.419334686865533	23.131604976833774
215-219	21.199802176063304	26.627205997188817	29.68197199229528	22.4910198344526
220-224	19.2122963194336	25.988442917382475	28.635899838617313	26.163360924566607
225-229	22.93560310271227	25.575147066479254	28.15019001509709	23.339059815711387
230-234	20.915924826904057	25.45015357384559	29.455255349055133	24.17866625019522
235-239	21.312665937841636	26.07756780675725	26.226039877140924	26.383726378260192
240-244	21.866312665937844	29.205476599510643	26.343849237336663	22.584361497214847
245-249	22.923889843302618	26.339111874642096	25.82778905721276	24.90920922484252
250-254	20.57785413087615	25.93216721328544	24.51855900879796	28.97141964704045
255-259	21.371076058097767	27.554479670987558	25.525586964443754	25.548857306470925
260-264	19.050028632411887	26.80358165443282	27.46233536363163	26.684054349523663
265-269	19.23254724347962	27.090530480503933	26.658857827060235	27.01806444895622
270-274	18.906824356720474	29.816924263066408	25.01786923726859	26.258382142944527
275-279	19.213451618226674	27.55371313119534	25.950505466450863	27.282329784127118
280-284	16.597792701337916	28.943255765526576	29.594460929772502	24.864490603363006
285-289	19.257795824873757	27.772814826383467	26.3135509396637	26.65583840907908
290-294	19.523712842938206	28.890832422302047	26.153105315216823	25.43234941954292
295-299	18.525908962418118	28.763875664467527	25.72628753515932	26.98392783795504
300-301	13.767895257431412	29.432427507938986	31.139569993232342	25.660107241397263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	1.0
24	1.0
25	0.25
26	0.5
27	0.75
28	1.0
29	3.0
30	5.0
31	21.25
32	48.25
33	138.75
34	239.5
35	436.25
36	881.75
37	1860.25
38	3373.5
39	5199.0
40	6987.5
41	8371.5
42	9715.25
43	11063.75
44	12059.75
45	11917.0
46	11485.0
47	10724.0
48	8915.25
49	6796.25
50	5282.75
51	3505.25
52	1760.25
53	803.0
54	423.75
55	285.0
56	293.5
57	332.5
58	386.25
59	476.25
60	606.5
61	758.75
62	806.0
63	728.75
64	575.75
65	359.75
66	187.5
67	85.5
68	52.0
69	29.0
70	16.0
71	12.0
72	8.0
73	7.25
74	4.25
75	3.75
76	3.25
77	1.25
78	2.25
79	0.75
80	0.75
81	1.25
82	1.0
83	0.5
84	0.25
85	0.25
86	1.25
87	0.75
88	1.0
89	0.75
90	1.0
91	0.25
92	1.25
93	0.5
94	0.25
95	0.25
96	0.0
97	0.25
98	0.75
99	1.0
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	5.2058930709563226E-5
275-279	5.2058930709563226E-5
280-284	0.0
285-289	0.0
290-294	0.0
295-299	5.2058930709563226E-5
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	384180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.868077686625696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.95196725912567	8.31898195928218
2	7.718370346310653	2.2951465984618973
3	5.4626391336770155	2.4365682904053467
4	4.531061823305122	2.694727167672177
5	4.637333682936121	3.4474118728350147
6	3.1851214073588334	2.8413979515567425
7	2.775600336096527	2.8887488996875295
8	2.4203643653968188	2.8788932331728323
9	1.8300834838753606	2.448884107014252
>10	11.01483868122722	29.614151333227294
>50	0.36128092670978335	3.362721046415477
>100	0.05299792048163343	1.4437174185334118
>500	0.012762013577961027	1.2363346929788677
>1k	0.03099346154645856	12.303983347724865
>5k	0.009115723984252517	10.455309239829386
>10k+	0.00546943439055151	11.333022841202636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	16224	4.223020459159769	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	15311	3.9853714404706126	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	10274	2.674267270550263	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	9856	2.5654641053672758	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	9721	2.5303243271383207	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	7237	1.8837524077255454	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6131	1.5958665209016607	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	5626	1.4644177208600135	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA	4950	1.28845853506169	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	4914	1.2790879275339684	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	4657	1.2121922015721798	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	4552	1.184861262949659	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	3478	0.9053048050393044	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3170	0.8251340517465771	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	2509	0.6530792857514707	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	2455	0.6390233744598885	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	2294	0.5971159352386902	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1968	0.5122598781821021	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1965	0.5114789942214587	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1910	0.4971627882763288	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA	1579	0.41100525795200166	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1341	0.34905513040762143	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1321	0.3438492373366651	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	1250	0.32536831693477014	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1078	0.2805976365245458	No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG	691	0.17986360560154094	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	680	0.17700036441251496	No Hit
GGGGGGAGCGAAAGCAGGAGTGGACAGATTCTACAGGACATGCAAGTTAG	671	0.17465771253058462	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	661	0.17205476599510644	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	652	0.16971211411317613	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	613	0.15956062262481127	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	593	0.15435472955385499	No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG	403	0.1048987453797699	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	396	0.10307668280493518	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	7.808839606434484E-4	0.0	0.0
9	7.808839606434484E-4	0.0	0.0
10-14	0.00161382685199646	0.0	0.0
15-19	0.0026029465354781615	0.0	0.0
20-24	0.003383830496121609	0.0	0.0
25-29	0.003592066218959863	0.0	0.0
30-34	0.005518246655213702	0.0	0.0
35-39	0.006142953823728461	0.0	0.0
40-44	0.007704721745015357	0.0	0.0
45-49	0.009422666458430944	0.0	0.0
50-54	0.01077619865687959	0.0	0.0
55-59	0.014212088083710758	0.0	0.0
60-64	0.019313863293247958	0.0	0.0
65-69	0.030818886980061428	0.0	0.0
70-74	0.04341714821177574	0.0	0.0
75-79	0.06491748659482534	0.0	0.0
80-84	0.09636108074340152	0.0	0.0
85-89	0.13441615909209226	0.0	0.0
90-94	0.17944713415586444	0.0	0.0
95-99	0.23421312926232493	0.0	0.0
100-104	0.2932479566869697	0.0	0.0
105-109	0.3603519183715966	0.0	0.0
110-114	0.43484824821698165	0.0	0.0
115-119	0.5188713623822165	0.0	0.0
120-124	0.6200218647508982	0.0	0.0
125-129	0.7314279764693633	0.0	0.0
130-134	0.8387214326617732	0.0	0.0
135-139	0.959602269769379	0.0	0.0
140-144	1.0849601749180071	0.0	0.0
145-149	1.2348378364308399	0.0	0.0
150-154	1.389244624915404	0.0	0.0
155-159	1.5636941017231507	0.0	0.0
160-164	1.7642771617470978	0.0	0.0
165-169	1.99281586756208	0.0	0.0
170-174	2.2368160757978033	0.0	0.0
175-179	2.4914883648289865	0.0	0.0
180-184	2.7502733093862255	0.0	0.0
185-189	3.047217450153574	0.0	0.0
190-194	3.3470768910406585	0.0	0.0
195-199	3.6647925451611227	0.0	0.0
200-204	3.968816700504972	0.0	0.0
205-209	4.283512936644281	0.0	0.0
210-214	4.654484876880629	0.0	0.0
215-219	4.968244052267166	0.0	0.0
220-224	5.298870321203602	0.0	0.0
225-229	5.628403352595138	0.0	0.0
230-234	5.93789369566349	0.0	0.0
235-239	6.220365453693581	0.0	0.0
240-244	6.4720703836743185	0.0	0.0
245-249	6.6757769795408395	0.0	0.0
250-254	6.851527929616326	0.0	0.0
255-259	7.006507366338695	0.0	0.0
260-264	7.142693529074913	0.0	0.0
265-269	7.2514966942579	0.0	0.0
270-274	7.338539226404289	0.0	0.0
275-279	7.4041334790983395	0.0	0.0
280-284	7.44817533447863	0.0	0.0
285-289	7.485501587797385	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8855	0.0	292.00168	1
GGGTTAT	8915	0.0	290.36737	2
GGTTATT	9085	0.0	284.60925	3
GGGGGGA	7010	0.0	283.42725	1
TTATTAG	9060	0.0	282.95255	5
ATTAGTA	9055	0.0	282.9459	7
TATTAGT	9125	0.0	280.77533	6
TAGTAGA	9190	0.0	280.71545	9
GTTATTA	9230	0.0	279.0195	4
TTAGTAG	9325	0.0	277.126	8
GGAGCGA	5590	0.0	269.66907	5
CGAAAGC	5620	0.0	269.01688	9
GCGAAAG	5690	0.0	267.26276	8
GGGGGAG	7470	0.0	266.96118	2
GAGCGAA	5625	0.0	266.68	6
GGGGAGC	8245	0.0	241.68889	3
AGCGAAA	6230	0.0	241.25603	7
GGGAGCG	6350	0.0	237.62599	4
GCAAAAG	2540	0.0	176.53543	8
GAGCAAA	2610	0.0	174.62643	6
>>END_MODULE