FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30304_flu.3520000003baad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30304_flu.3520000003baad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384180
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA162244.223020459159769No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC153113.9853714404706126No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC102742.674267270550263No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG98562.5654641053672758No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA97212.5303243271383207No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG72371.8837524077255454No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG61311.5958665209016607No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG56261.4644177208600135No Hit
GGGGGGAGCGAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA49501.28845853506169No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC49141.2790879275339684No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT46571.2121922015721798No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA45521.184861262949659No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA34780.9053048050393044No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA31700.8251340517465771No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT25090.6530792857514707No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA24550.6390233744598885No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT22940.5971159352386902No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG19680.5122598781821021No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG19650.5114789942214587No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC19100.4971627882763288No Hit
GGGGGGAGCAAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA15790.41100525795200166No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG13410.34905513040762143No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13210.3438492373366651No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT12500.32536831693477014Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA10780.2805976365245458No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG6910.17986360560154094No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT6800.17700036441251496No Hit
GGGGGGAGCGAAAGCAGGAGTGGACAGATTCTACAGGACATGCAAGTTAG6710.17465771253058462No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC6610.17205476599510644No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6520.16971211411317613No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA6130.15956062262481127No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA5930.15435472955385499No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG4030.1048987453797699No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3960.10307668280493518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA88550.0292.001681
GGGTTAT89150.0290.367372
GGTTATT90850.0284.609253
GGGGGGA70100.0283.427251
TTATTAG90600.0282.952555
ATTAGTA90550.0282.94597
TATTAGT91250.0280.775336
TAGTAGA91900.0280.715459
GTTATTA92300.0279.01954
TTAGTAG93250.0277.1268
GGAGCGA55900.0269.669075
CGAAAGC56200.0269.016889
GCGAAAG56900.0267.262768
GGGGGAG74700.0266.961182
GAGCGAA56250.0266.686
GGGGAGC82450.0241.688893
AGCGAAA62300.0241.256037
GGGAGCG63500.0237.625994
GCAAAAG25400.0176.535438
GAGCAAA26100.0174.626436
Produced by FastQC (version 0.11.2)