##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30301_flu.3520000003b9e1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345555
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15121181866852	34.0	34.0	34.0	34.0	34.0
2	33.46973419571414	34.0	34.0	34.0	34.0	34.0
3	33.45734832371113	34.0	34.0	34.0	34.0	34.0
4	33.491797253693335	34.0	34.0	34.0	34.0	34.0
5	33.48411974938866	34.0	34.0	34.0	34.0	34.0
6	37.03271548668085	38.0	38.0	38.0	36.0	38.0
7	36.18064852194296	38.0	38.0	38.0	34.0	38.0
8	36.94373399314147	38.0	38.0	38.0	36.0	38.0
9	37.0134566132743	38.0	38.0	38.0	36.0	38.0
10-14	37.03095889221687	38.0	38.0	38.0	36.8	38.0
15-19	36.93484163157819	38.0	38.0	38.0	36.6	38.0
20-24	37.02874795618642	38.0	38.0	38.0	36.8	38.0
25-29	36.95235374976487	38.0	38.0	38.0	36.2	38.0
30-34	36.601646047662456	38.0	38.0	38.0	34.6	38.0
35-39	36.87575176165878	38.0	38.0	38.0	36.0	38.0
40-44	36.42140324984445	38.0	37.6	38.0	34.0	38.0
45-49	36.73860601062059	38.0	38.0	38.0	35.2	38.0
50-54	36.704203382963634	38.0	38.0	38.0	35.2	38.0
55-59	36.3613126709207	38.0	37.4	38.0	33.8	38.0
60-64	36.54490254807483	38.0	37.2	38.0	34.2	38.0
65-69	36.416883564121484	38.0	37.0	38.0	34.0	38.0
70-74	36.28378753020503	38.0	37.0	38.0	34.0	38.0
75-79	36.31966315058385	38.0	37.0	38.0	34.0	38.0
80-84	35.65346355862308	38.0	37.0	38.0	30.2	38.0
85-89	35.869663584668146	38.0	37.0	38.0	32.0	38.0
90-94	35.85529423680746	38.0	37.0	38.0	32.0	38.0
95-99	34.928825801970746	38.0	36.0	38.0	27.0	38.0
100-104	35.288353518253246	38.0	36.0	38.0	28.4	38.0
105-109	35.11717382182286	38.0	35.8	38.0	27.4	38.0
110-114	34.64728972233074	38.0	35.2	38.0	24.0	38.0
115-119	34.67847607472038	38.0	35.0	38.0	24.4	38.0
120-124	34.19526616602277	37.6	34.6	38.0	23.6	38.0
125-129	33.76740895081825	37.0	34.0	38.0	23.0	38.0
130-134	33.60231569504132	37.0	33.6	38.0	23.0	38.0
135-139	32.942555020184926	37.0	31.2	38.0	21.2	38.0
140-144	31.676005266889497	36.0	27.6	38.0	17.4	38.0
145-149	31.490459116493753	35.0	28.0	37.6	18.0	38.0
150-154	30.485496664785636	35.0	25.4	37.0	14.2	38.0
155-159	30.34985979077137	35.0	25.4	37.0	14.8	37.4
160-164	29.579846334158095	34.4	24.2	37.0	10.2	37.4
165-169	28.464121775115395	32.8	22.6	36.6	9.8	37.0
170-174	27.838175688385355	32.2	21.4	36.2	9.4	37.0
175-179	26.975995717034913	29.8	20.0	35.4	9.2	37.0
180-184	26.084284701422348	28.2	20.0	35.0	9.0	37.0
185-189	25.357297680542892	27.0	18.2	35.0	9.0	36.4
190-194	24.30743702160293	25.6	16.0	34.6	8.4	36.0
195-199	23.461850646062135	24.6	15.0	33.6	8.0	36.0
200-204	22.574234492338412	23.4	14.0	32.0	8.0	36.0
205-209	21.656304785055926	22.6	10.2	30.6	8.0	35.2
210-214	20.390694968962972	20.4	9.0	28.2	8.0	35.0
215-219	19.83129516285396	20.0	9.0	27.8	8.0	34.0
220-224	19.072391370404134	18.6	9.0	26.2	8.0	33.4
225-229	18.271599021863377	16.8	9.0	25.4	8.0	32.4
230-234	17.375952308604997	15.8	9.0	24.6	8.0	31.2
235-239	16.34784969107667	14.6	8.4	22.8	7.2	29.2
240-244	15.51738276106553	13.4	8.0	21.2	7.0	28.2
245-249	14.80381646915831	12.8	8.0	20.2	7.0	26.6
250-254	14.093593205133772	11.8	8.0	19.0	7.0	25.4
255-259	13.518301283442579	11.2	8.0	18.0	7.0	24.4
260-264	12.92527962263605	10.2	8.0	16.4	7.0	23.2
265-269	12.513910086672166	9.0	7.4	15.8	7.0	22.4
270-274	12.01799134725297	9.0	7.4	14.4	7.0	21.0
275-279	11.543404667853164	8.0	7.0	13.8	7.0	20.4
280-284	11.388712650663425	8.0	7.0	13.4	7.0	19.6
285-289	11.125257629031559	8.0	7.0	13.0	7.0	19.0
290-294	10.858842731258411	8.0	7.0	13.0	7.0	18.8
295-299	10.592954522434923	8.0	7.0	13.0	7.0	18.0
300-301	10.321284600136014	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2745556023616871
1101	2	0.11515402628309346
1101	3	0.13405604338789345
1101	4	0.15522852427259437
1101	5	0.10565363912665049
1101	6	0.1975963165134047
1101	7	1.10369864968537
1101	8	0.37558032411729414
1101	9	0.2902623500957944
1101	10-14	0.17645114431071107
1101	15-19	0.345162280326889
1101	20-24	0.34073562109733047
1101	25-29	0.25203803574914474
1101	30-34	0.6043941859661928
1101	35-39	0.3790161668981895
1101	40-44	0.5863008207117772
1101	45-49	0.2616087800718532
1101	50-54	0.3239872507619097
1101	55-59	0.4901936641405129
1101	60-64	0.3715326442125786
1101	65-69	0.38083365071792485
1101	70-74	0.39285768106093855
1101	75-79	0.40923601271443033
1101	80-84	0.724521305130267
1101	85-89	0.38226594706384276
1101	90-94	0.44315800240762115
1101	95-99	1.059396285480915
1101	100-104	0.5390026455713652
1101	105-109	0.68868929131974
1101	110-114	0.6535033109124129
1101	115-119	0.5781027310323452
1101	120-124	0.9844166173081845
1101	125-129	0.8221593064224066
1101	130-134	0.8308533337187711
1101	135-139	0.5904845691586686
1101	140-144	1.3473087843284794
1101	145-149	1.3773232885606213
1101	150-154	1.2594023062157937
1101	155-159	0.7696202378929407
1101	160-164	1.2527998899249155
1101	165-169	0.8801535860904579
1101	170-174	0.7182426259700456
1101	175-179	0.9618348720244292
1101	180-184	0.7939026136405545
1101	185-189	0.9097816121604936
1101	190-194	0.8315155157700751
1101	195-199	1.0044825042454555
1101	200-204	1.3730290496929811
1101	205-209	0.960193529654827
1101	210-214	0.8628184565950185
1101	215-219	1.032807753945626
1101	220-224	0.6211458954865492
1101	225-229	0.43412396187744307
1101	230-234	0.7081704972332687
1101	235-239	0.4347965923006356
1101	240-244	0.4197780852437205
1101	245-249	0.07784851451821595
1101	250-254	0.27891677516000435
1101	255-259	0.10350721642217131
1101	260-264	0.17602316389364958
1101	265-269	0.2822173874571181
1101	270-274	0.0011965010365457829
1101	275-279	0.22977229554467904
1101	280-284	0.041175324070806596
1101	285-289	0.13825240612323242
1101	290-294	-0.02038295960697667
1101	295-299	-0.02286318834625689
1101	300-301	-0.1541755273504961
1102	1	0.038947313322282184
1102	2	-0.02473908217994847
1102	3	0.11693068577013577
1102	4	0.06655763518264024
1102	5	0.047162453343695176
1102	6	-0.06140014363548829
1102	7	0.9783825479418198
1102	8	0.196165187132749
1102	9	0.07838046561045076
1102	10-14	0.14459810124761674
1102	15-19	0.2783456675699867
1102	20-24	0.18710144315723198
1102	25-29	0.15382749825101882
1102	30-34	0.5145076101707744
1102	35-39	0.23296891922704788
1102	40-44	0.551733118464675
1102	45-49	0.2970993589883051
1102	50-54	0.3094119535255331
1102	55-59	0.37658605645223986
1102	60-64	0.222550214035806
1102	65-69	0.3128018396793095
1102	70-74	0.310605412637905
1102	75-79	0.42522847145178844
1102	80-84	0.8999255063071274
1102	85-89	0.4520731784216352
1102	90-94	0.4641262780642066
1102	95-99	1.0083811577764195
1102	100-104	0.7167837609114116
1102	105-109	1.0061569118779872
1102	110-114	0.8191375718845464
1102	115-119	0.6542072097560094
1102	120-124	0.9111083645342504
1102	125-129	0.827311932737949
1102	130-134	0.8652014894917599
1102	135-139	0.9326845029772528
1102	140-144	1.923697647778635
1102	145-149	1.9869877292360663
1102	150-154	1.9000953306699522
1102	155-159	1.4908130663670711
1102	160-164	1.630979559042249
1102	165-169	1.422715197693453
1102	170-174	1.486762250225656
1102	175-179	1.703705119814007
1102	180-184	1.2807609622066032
1102	185-189	1.6470262428571871
1102	190-194	1.594653931396703
1102	195-199	1.3208306575763196
1102	200-204	1.6064875416114077
1102	205-209	1.080329164929278
1102	210-214	1.892781299758493
1102	215-219	1.5610167895407265
1102	220-224	1.4389306251623175
1102	225-229	0.9300518509954863
1102	230-234	1.109135134132952
1102	235-239	1.021353461309051
1102	240-244	0.9011074769974208
1102	245-249	0.3208212568099711
1102	250-254	1.0225192177671634
1102	255-259	0.7587320683992527
1102	260-264	0.6043812908374271
1102	265-269	0.3625359862670763
1102	270-274	0.23688269366354575
1102	275-279	0.20233631263462648
1102	280-284	0.23832317360210098
1102	285-289	0.25297703892015555
1102	290-294	-0.03655466985935618
1102	295-299	-0.05135905078659242
1102	300-301	-0.09972787966031404
1103	1	-0.004698171627552483
1103	2	0.03780272384011596
1103	3	-0.03465794299241054
1103	4	0.018731548226114114
1103	5	0.0478592203448045
1103	6	0.20562884187175712
1103	7	0.9047760819440498
1103	8	0.24817187609596658
1103	9	0.2129122381857016
1103	10-14	0.018516718861881998
1103	15-19	0.1263947199668678
1103	20-24	0.12711259142924547
1103	25-29	-0.012359792718882545
1103	30-34	0.3630081675843684
1103	35-39	0.022768250330727824
1103	40-44	0.381643932288533
1103	45-49	0.15354863435062782
1103	50-54	0.06339746077190966
1103	55-59	0.19616799625156744
1103	60-64	0.17255578817180606
1103	65-69	0.18410618750539243
1103	70-74	0.1616254795275367
1103	75-79	0.14048488170819695
1103	80-84	0.7413580592614153
1103	85-89	0.3775023868943137
1103	90-94	0.21925448319240814
1103	95-99	0.9378014476314931
1103	100-104	0.4946488668200004
1103	105-109	0.638052118121017
1103	110-114	0.5889369029882232
1103	115-119	0.5446475665007
1103	120-124	0.8984996688820814
1103	125-129	0.8886162805640296
1103	130-134	0.8214055028696805
1103	135-139	0.7806833881500381
1103	140-144	1.1539429097630354
1103	145-149	1.5048584092806543
1103	150-154	1.947397448841631
1103	155-159	0.9001385959099935
1103	160-164	0.818058711773574
1103	165-169	1.5534454095106192
1103	170-174	1.6733285824441637
1103	175-179	1.5566036705386423
1103	180-184	1.2507107949825276
1103	185-189	1.2542001559006692
1103	190-194	1.0546985244847775
1103	195-199	1.7037793755250412
1103	200-204	1.659218868255774
1103	205-209	0.9968286075156776
1103	210-214	1.542591779134188
1103	215-219	1.7599800002430683
1103	220-224	1.5991981836907492
1103	225-229	0.9546449390668315
1103	230-234	1.7108296714796651
1103	235-239	0.9620390800381458
1103	240-244	0.8878577557042213
1103	245-249	1.4311891497865599
1103	250-254	0.5462483147571273
1103	255-259	0.7238769959354823
1103	260-264	0.48043480254311
1103	265-269	0.3681602895000733
1103	270-274	0.5446697616680058
1103	275-279	0.46301078309170585
1103	280-284	0.32161748798337264
1103	285-289	0.18878528864144783
1103	290-294	0.25059137250407915
1103	295-299	0.05262088232377593
1103	300-301	-0.10854894989442698
2116	1	-0.08476191437324587
2116	2	-0.002564510485825622
2116	3	0.10713874619035124
2116	4	0.015563438626649884
2116	5	0.050297142521571914
2116	6	0.16342781437675313
2116	7	0.9250477021514527
2116	8	0.2209670430639008
2116	9	0.16300975507277116
2116	10-14	0.14361280867255743
2116	15-19	0.07900626977183833
2116	20-24	0.17338126234543694
2116	25-29	0.0909805174323921
2116	30-34	0.458211763816891
2116	35-39	-0.06414545911652425
2116	40-44	0.47381499117921066
2116	45-49	0.217623888845921
2116	50-54	0.2611640862119273
2116	55-59	0.3934284852630796
2116	60-64	0.3346369412495349
2116	65-69	0.3620169476614308
2116	70-74	0.3231900309498599
2116	75-79	0.5200439151017875
2116	80-84	0.7915250271997678
2116	85-89	0.48770788589536096
2116	90-94	0.5819840046461309
2116	95-99	1.2611988408386097
2116	100-104	0.5654475539097135
2116	105-109	0.6374879472524029
2116	110-114	0.5993612203700138
2116	115-119	0.749815961515452
2116	120-124	0.8150756369155658
2116	125-129	0.9316217034543399
2116	130-134	0.9761343583544146
2116	135-139	1.3263055934587697
2116	140-144	1.607687558226548
2116	145-149	1.833267694791072
2116	150-154	1.951955530850956
2116	155-159	1.9550339436409416
2116	160-164	1.7993516429884906
2116	165-169	2.093756036472854
2116	170-174	1.759642919805021
2116	175-179	2.0463905604634895
2116	180-184	2.2409272349026104
2116	185-189	1.5397386393941517
2116	190-194	1.6745177614746005
2116	195-199	1.1654942456610904
2116	200-204	0.8011463727748449
2116	205-209	1.0993783173434757
2116	210-214	1.7063307192921187
2116	215-219	1.9767900088055264
2116	220-224	1.017333470533103
2116	225-229	0.7007509232738549
2116	230-234	1.1855456832768283
2116	235-239	0.917801138833692
2116	240-244	0.8146254276669254
2116	245-249	0.7705764583204786
2116	250-254	1.004134814614421
2116	255-259	1.6179398824353406
2116	260-264	0.5113871381914805
2116	265-269	0.6052038628993692
2116	270-274	0.5729682109116538
2116	275-279	0.7190782362360295
2116	280-284	0.24202039921922136
2116	285-289	0.02967007600706495
2116	290-294	0.23799409346307243
2116	295-299	0.27257997280041835
2116	300-301	-0.17348211514987355
1117	1	0.13904706101928355
1117	2	0.15856931422913334
1117	3	0.13142710658806322
1117	4	0.0978314719484672
1117	5	0.24117539120674536
1117	6	0.14083097764597596
1117	7	1.020679972104233
1117	8	0.24105898517300517
1117	9	0.2566097973008823
1117	10-14	0.24795798507088307
1117	15-19	0.2491826680980722
1117	20-24	0.2740271604605198
1117	25-29	0.29042243458852113
1117	30-34	0.53225683272548
1117	35-39	0.3439829719173062
1117	40-44	0.5604368384670195
1117	45-49	0.2903392521996011
1117	50-54	0.28563239593590595
1117	55-59	0.38717104735759733
1117	60-64	0.3745180307347411
1117	65-69	0.39618284654429914
1117	70-74	0.3648710935626198
1117	75-79	0.5354658122955342
1117	80-84	0.8797390661264117
1117	85-89	0.4526892671383962
1117	90-94	0.5125801887606869
1117	95-99	1.1143880227649774
1117	100-104	0.45548220015333385
1117	105-109	0.40535379622932766
1117	110-114	0.7759658884954703
1117	115-119	0.6652234627630946
1117	120-124	0.9234272051139527
1117	125-129	0.9205708953618981
1117	130-134	0.9628852892922666
1117	135-139	0.9559007794543959
1117	140-144	1.4128940920666118
1117	145-149	1.823229881439314
1117	150-154	2.1852864648599386
1117	155-159	1.7949841336674268
1117	160-164	2.1526372777537404
1117	165-169	1.4623546391063513
1117	170-174	1.4671024192099935
1117	175-179	1.4608656842686258
1117	180-184	1.8053274997880138
1117	185-189	1.1679167844285097
1117	190-194	-0.9898705067890603
1117	195-199	1.5264510002389997
1117	200-204	1.68081497809559
1117	205-209	1.6900017577826496
1117	210-214	1.8485967371814809
1117	215-219	1.8257904502812075
1117	220-224	1.9473351020736622
1117	225-229	1.3976140466183224
1117	230-234	0.9633010673606748
1117	235-239	1.0270486147444764
1117	240-244	0.7887578319818722
1117	245-249	1.0862330094356842
1117	250-254	0.7132925557945011
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2108	245-249	-0.9668670057210011
2108	250-254	-0.7100241051884062
2108	255-259	-0.6722066738926706
2108	260-264	-0.45681188728691957
2108	265-269	-0.2798503218249344
2108	270-274	-0.5707349711707597
2108	275-279	-0.3523732905302328
2108	280-284	-0.6337262062602189
2108	285-289	-1.0934703222140314
2108	290-294	-0.6449659568226203
2108	295-299	-0.504012604388592
2108	300-301	2.6078105674311445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	22.0
11	187.0
12	442.0
13	547.0
14	698.0
15	1158.0
16	1603.0
17	2519.0
18	2985.0
19	3505.0
20	4799.0
21	6527.0
22	9096.0
23	12337.0
24	18168.0
25	25958.0
26	36795.0
27	49838.0
28	58638.0
29	54498.0
30	36379.0
31	15108.0
32	3412.0
33	323.0
34	7.0
35	2.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.694419122860324	23.184731808250493	14.591888411396159	33.528960657493016
2	43.82399328616284	24.72746740750387	17.318805978787747	14.129733327545543
3	42.78566364254605	23.58814081694665	19.13414651792045	14.492049022586853
4	43.18791509311109	22.378492569923747	19.320223987498373	15.1133683494668
5	27.554513753237547	22.653412626065315	35.185426343129166	14.60664727756797
6	26.564222772062333	21.851514230730274	35.618642473701726	15.965620523505663
7	16.991795806745667	47.06226215797775	20.488489531333652	15.457452503942932
8	25.854929027217082	20.91794359798006	36.87980205755958	16.347325317243275
9	15.717613693912691	19.682539682539684	37.93896774753657	26.660878876011058
10-14	26.070176961699293	33.89492266064737	23.90930532042656	16.12559505722678
15-19	21.053898800480386	35.268075993691305	22.116132019504857	21.56189318632345
20-24	25.17584754959413	30.821374310891176	26.417155011503233	17.58562312801146
25-29	19.34441695243883	25.77789353359089	37.34919187972971	17.52849763424057
30-34	19.789266542229168	28.206508370592235	31.13165776793853	20.872567319240062
35-39	20.599383600295177	29.913732980278102	27.92840502959008	21.558478389836637
40-44	20.36723531709858	28.762020517717872	29.176368450753138	21.694375714430407
45-49	21.001866562486434	30.25226085572485	26.45002966242711	22.295842919361604
50-54	21.947707311426544	29.559693825874316	27.78828261781771	20.704316244881422
55-59	21.431841530291848	27.11562558782249	29.8881509455803	21.564381936305363
60-64	22.312048733197322	25.86922487013645	28.529351333362275	23.28937506330396
65-69	20.132019504854508	27.455021631867577	28.85074734846841	23.56221151480951
70-74	20.8487794996455	26.506229109693102	29.483468622939906	23.16152276772149
75-79	20.186366859110706	26.432955679993054	30.312685390169435	23.0679920707268
80-84	22.954319862250582	26.073996903532	27.824398431508733	23.147284802708686
85-89	21.02386016697776	27.82445630941529	26.51114873175037	24.640534791856577
90-94	20.149672266354127	29.934742660357976	28.927840720001157	20.987744353286743
95-99	22.11769472298187	29.955810218344403	26.871901723314668	21.05459333535906
100-104	22.65648015511279	28.12889409790048	26.95860282733574	22.256022919651
105-109	23.48818567232423	27.541143956823085	24.177337905687953	24.793332465164735
110-114	21.57532086064447	27.914861599455946	29.502163186757535	21.007654353142048
115-119	23.40032701017204	27.66870686287277	27.274558319225594	21.656407807729593
120-124	18.45824832515808	28.873319732025294	27.966199302571226	24.702232640245402
125-129	20.769776157196393	25.045564381936302	30.30371431465324	23.88094514621406
130-134	24.746798628293615	28.77776330830114	26.26597791957865	20.2094601438266
135-139	24.09248889467668	27.327227214191662	25.154201212542144	23.426082678589516
140-144	21.11281850935452	28.878644499428457	26.845740909551303	23.162796081665725
145-149	21.16791827639594	26.661110387637276	29.083590166543676	23.0873811694231
150-154	23.031065966344	25.465931617253407	25.828536701827492	25.674465714575106
155-159	21.59528873840633	26.680152218894243	30.207521233956967	21.517037808742458
160-164	25.287320397621215	28.3839620320933	26.006163997048226	20.322553573237258
165-169	20.588213164329847	25.42599586172968	28.487100461576304	25.49869051236417
170-174	22.630782364601874	28.59087554803143	26.373399314146805	22.404942773219894
175-179	20.717686041295885	28.700149035609385	28.66304929750691	21.919115625587825
180-184	20.357511828797154	29.051467928405028	26.67187567825672	23.9191445645411
185-189	22.498010446962134	27.31298924917886	27.673915874462825	22.51508442939619
190-194	21.16120443923543	27.654642531579633	26.32206161103153	24.862091418153405
195-199	21.826453097191475	29.20964824702291	27.33590890017508	21.62798975561054
200-204	22.798338904081838	27.496057067615865	27.04327820462734	22.66232582367496
205-209	22.333405680716528	27.095831343780297	27.648854740924023	22.921908234579156
210-214	24.071942237849257	26.802679747073547	27.21789584870715	21.907482166370045
215-219	24.019794244042192	26.894300473151887	27.183921517558712	21.90198376524721
220-224	21.14303077657681	26.717714980249163	27.733182850776288	24.406071392397738
225-229	23.045998466235478	27.886443547336892	26.03608687473774	23.031471111689893
230-234	22.39996527325607	26.82380518296653	27.61464889814935	23.161580645628046
235-239	23.25210169148182	26.87959948488663	25.087583742096047	24.7807150815355
240-244	24.233653108767054	30.1468073099796	22.967400269132266	22.65213931212108
245-249	22.76864753801855	28.93715906295669	23.62576145620813	24.66843194281663
250-254	23.05068657666652	27.072911692784075	24.80803345342999	25.06836827711942
255-259	23.122686692422334	27.733993141468073	25.92449827089754	23.21882189521205
260-264	23.054043495246777	28.52564714734268	25.08769949790916	23.332609859501382
265-269	22.206305797919292	28.196727004384254	25.59129516285396	24.005672034842497
270-274	21.7410831850212	29.76087742906339	23.63930488634226	24.858734499573153
275-279	22.48822908075415	29.12288926509528	23.244693319442636	25.144188334707934
280-284	20.523332031080436	29.804922515952597	25.346240106495348	24.32550534647162
285-289	21.868703968977442	29.022297463500745	25.517500831994905	23.591497735526907
290-294	21.28818856621956	30.619901318169322	23.651632880438715	24.440277235172402
295-299	20.520553891565743	31.73312497286973	22.075385973289347	25.670935162275182
300-301	16.448756348482878	33.150439148615995	29.53191243072738	20.86889207217375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.25
27	0.25
28	1.0
29	0.25
30	1.0
31	1.75
32	3.25
33	7.25
34	25.25
35	89.25
36	304.5
37	928.0
38	2081.75
39	3416.75
40	4793.25
41	6411.25
42	9031.25
43	12234.75
44	14062.5
45	13568.75
46	11644.75
47	9381.25
48	7458.25
49	5987.0
50	4662.5
51	3558.5
52	2555.5
53	1413.0
54	631.75
55	254.75
56	118.25
57	70.0
58	54.75
59	48.75
60	45.25
61	38.5
62	31.0
63	29.0
64	20.25
65	14.75
66	11.0
67	6.25
68	6.5
69	4.0
70	4.5
71	4.25
72	1.75
73	2.25
74	2.25
75	3.0
76	1.0
77	1.0
78	1.5
79	1.75
80	0.75
81	1.25
82	1.0
83	0.5
84	1.0
85	1.5
86	1.25
87	0.25
88	0.75
89	0.75
90	0.5
91	0.5
92	0.25
93	0.0
94	0.25
95	0.75
96	0.75
97	0.0
98	0.0
99	1.0
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	345555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.717176733885303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.67846864441476	6.677311575303315
2	4.891671686671276	1.341998501004254
3	3.537355405905096	1.4556758782009436
4	3.528714069502062	1.9361597813882965
5	4.101536665783867	2.8130751662533986
6	3.8919756598255346	3.203215078188403
7	3.792096210065404	3.6411797733754656
8	3.762775143447624	4.129172132203334
9	3.286155668705585	4.056910027442419
>10	20.16608047178643	45.34947848031541
>50	0.2803624180993804	2.454112215405214
>100	0.032805384072486385	0.8714281094390746
>500	0.013044149144496892	1.281130917972806
>1k	0.028262323146379564	10.481058483013559
>5k	0.0065220745722414375	6.526729811621089
>10k+	0.002174024857413813	3.7813640688730423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	12680	3.6694592756579993	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9663	2.7963710552589314	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6933	2.006337630767895	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5290	1.5308706284093703	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4955	1.4339251349278697	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4646	1.3445037692986646	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4175	1.2082012993590023	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4103	1.187365252998799	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3389	0.980741126593451	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2203	0.6375251407156602	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2046	0.5920909840691062	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1919	0.5553385134059701	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1896	0.5486825541520164	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1871	0.5414478158325013	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1695	0.4905152580631159	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1170	0.3385857533533012	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1078	0.31196191633748604	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	855	0.24742805052741243	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	722	0.2089392426675927	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT	687	0.19881060902027173	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	681	0.19707427182358817	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	678	0.19620610322524634	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	673	0.19475915556134335	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	482	0.13948575480024886	No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG	357	0.10331206320267396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.8938953278059935E-4	0.0	0.0
2	2.8938953278059935E-4	0.0	0.0
3	2.8938953278059935E-4	0.0	0.0
4	2.8938953278059935E-4	0.0	0.0
5	2.8938953278059935E-4	0.0	0.0
6	2.8938953278059935E-4	0.0	0.0
7	5.787790655611987E-4	0.0	0.0
8	5.787790655611987E-4	0.0	0.0
9	5.787790655611987E-4	0.0	0.0
10-14	8.681685983417981E-4	0.0	0.0
15-19	0.001273313944234637	0.0	0.0
20-24	0.0015627034770152363	0.0	0.0
25-29	0.0017363371966835962	0.0	0.0
30-34	0.003935697645816151	0.0	0.0
35-39	0.005151133683494668	0.0	0.0
40-44	0.007871395291632301	0.0	0.0
45-49	0.009781366207984258	0.0	0.0
50-54	0.01302252897512697	0.0	0.0
55-59	0.01672671499471864	0.0	0.0
60-64	0.023961453314233622	0.0	0.0
65-69	0.038546685766375835	0.0	0.0
70-74	0.05949848793969122	0.0	0.0
75-79	0.08953712144231743	0.0	0.0
80-84	0.1278522955824688	0.0	0.0
85-89	0.18758229514838448	0.0	0.0
90-94	0.2632287190172332	0.0	0.0
95-99	0.3546758113759025	0.0	0.0
100-104	0.46985284542258104	0.0	0.0
105-109	0.6066183386146923	0.0	0.0
110-114	0.7709915932340727	0.0	0.0
115-119	0.9550433360825339	0.0	0.0
120-124	1.1733587996122181	0.0	0.0
125-129	1.4349669372458798	0.0	0.0
130-134	1.7322857432246679	0.0	0.0
135-139	2.059411671079857	0.0	0.0
140-144	2.4086469592394844	0.0	0.0
145-149	2.828030270145129	0.0	0.0
150-154	3.318661284021357	0.0	0.0
155-159	3.8719740706978625	0.0	0.0
160-164	4.4658013919636526	0.0	0.0
165-169	5.108824933802144	0.0	0.0
170-174	5.8099000159164245	0.0	0.0
175-179	6.558145591873942	0.0	0.0
180-184	7.37231410339888	0.0	0.0
185-189	8.258540608586188	0.0	0.0
190-194	9.236908741010838	0.0	0.0
195-199	10.159424693608832	0.0	0.0
200-204	11.049934163881291	0.0	0.0
205-209	11.934308576058806	0.0	0.0
210-214	12.84339685433578	0.0	0.0
215-219	13.750633039602956	0.0	0.0
220-224	14.650287219111286	0.0	0.0
225-229	15.504275730346833	0.0	0.0
230-234	16.270868602682643	0.0	0.0
235-239	16.980104469621335	0.0	0.0
240-244	17.58012472688863	0.0	0.0
245-249	18.052119054853787	0.0	0.0
250-254	18.465077918131698	0.0	0.0
255-259	18.808699049355386	0.0	0.0
260-264	19.09484741936884	0.0	0.0
265-269	19.309805964318272	0.0	0.0
270-274	19.476667968919564	0.0	0.0
275-279	19.583915729768055	0.0	0.0
280-284	19.648160206045347	0.0	2.3151162622447947E-4
285-289	19.699440031254067	0.0	2.8938953278059935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5255	0.0	294.7193	1
GGGTTAT	5305	0.0	292.2196	2
GTTATTA	5370	0.0	287.03442	4
GGTTATT	5470	0.0	283.13528	3
TTATTAG	5535	0.0	279.01083	5
TTAGTAG	5565	0.0	277.50674	8
TATTAGT	5565	0.0	276.1815	6
TAGTAGA	5655	0.0	273.09018	9
GGGGGGA	3470	0.0	269.49567	1
ATTAGTA	5755	0.0	266.80713	7
GGGGAGC	3565	0.0	265.6241	3
GGAGCGA	2650	0.0	257.15094	5
GGGAGCG	2650	0.0	257.15094	4
GCGAAAG	2735	0.0	251.31628	8
GAGCGAA	2765	0.0	246.98914	6
CGAAAGC	2795	0.0	245.92128	9
AGCGAAA	2820	0.0	242.17197	7
GGGGGAG	4220	0.0	224.0462	2
GGGAGCA	1275	0.0	195.50981	4
CAAAAGC	1685	0.0	138.30861	9
>>END_MODULE