FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30301_flu.3520000003b9e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30301_flu.3520000003b9e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345555
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC126803.6694592756579993No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT96632.7963710552589314No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG69332.006337630767895No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA52901.5308706284093703No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG49551.4339251349278697No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA46461.3445037692986646No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT41751.2082012993590023No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA41031.187365252998799No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33890.980741126593451No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG22030.6375251407156602No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA20460.5920909840691062No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19190.5553385134059701No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG18960.5486825541520164No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA18710.5414478158325013No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16950.4905152580631159No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11700.3385857533533012No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10780.31196191633748604No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8550.24742805052741243No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7220.2089392426675927No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT6870.19881060902027173Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6810.19707427182358817No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6780.19620610322524634No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6730.19475915556134335No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4820.13948575480024886No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG3570.10331206320267396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA52550.0294.71931
GGGTTAT53050.0292.21962
GTTATTA53700.0287.034424
GGTTATT54700.0283.135283
TTATTAG55350.0279.010835
TTAGTAG55650.0277.506748
TATTAGT55650.0276.18156
TAGTAGA56550.0273.090189
GGGGGGA34700.0269.495671
ATTAGTA57550.0266.807137
GGGGAGC35650.0265.62413
GGAGCGA26500.0257.150945
GGGAGCG26500.0257.150944
GCGAAAG27350.0251.316288
GAGCGAA27650.0246.989146
CGAAAGC27950.0245.921289
AGCGAAA28200.0242.171977
GGGGGAG42200.0224.04622
GGGAGCA12750.0195.509814
CAAAAGC16850.0138.308619
Produced by FastQC (version 0.11.2)