##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n02_30295_flu.3520000003b977.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 320926 Sequences flagged as poor quality 0 Sequence length 301 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.271068719891815 34.0 34.0 34.0 34.0 34.0 2 33.46801443323383 34.0 34.0 34.0 34.0 34.0 3 33.48363797261674 34.0 34.0 34.0 34.0 34.0 4 33.48542966291294 34.0 34.0 34.0 34.0 34.0 5 33.422399556284006 34.0 34.0 34.0 34.0 34.0 6 36.95247502539526 38.0 38.0 38.0 36.0 38.0 7 36.03138418202327 38.0 38.0 38.0 34.0 38.0 8 36.846129637361884 38.0 38.0 38.0 35.0 38.0 9 36.93232396253342 38.0 38.0 38.0 36.0 38.0 10-14 36.94603553467154 38.0 38.0 38.0 36.4 38.0 15-19 36.86498756722734 38.0 38.0 38.0 36.4 38.0 20-24 36.977230888117504 38.0 38.0 38.0 36.6 38.0 25-29 36.89557530396415 38.0 38.0 38.0 36.0 38.0 30-34 36.56637231012757 38.0 38.0 38.0 34.6 38.0 35-39 36.84912658993039 38.0 38.0 38.0 36.0 38.0 40-44 36.3925041909973 38.0 37.6 38.0 34.0 38.0 45-49 36.707961336881404 38.0 38.0 38.0 35.2 38.0 50-54 36.677356150639085 38.0 38.0 38.0 35.2 38.0 55-59 36.34255124234247 38.0 37.4 38.0 33.8 38.0 60-64 36.530973495447554 38.0 37.6 38.0 34.2 38.0 65-69 36.39928893265114 38.0 37.4 38.0 34.0 38.0 70-74 36.28578052261269 38.0 37.0 38.0 34.0 38.0 75-79 36.3326280824863 38.0 37.0 38.0 34.0 38.0 80-84 35.67541426995631 38.0 37.0 38.0 30.6 38.0 85-89 35.900261119385775 38.0 37.0 38.0 32.0 38.0 90-94 35.8892305391274 38.0 37.0 38.0 32.0 38.0 95-99 34.992722933012594 38.0 36.2 38.0 27.0 38.0 100-104 35.35908277920767 38.0 36.2 38.0 28.4 38.0 105-109 35.18518100745966 38.0 35.8 38.0 27.8 38.0 110-114 34.74163140412431 38.0 35.2 38.0 24.2 38.0 115-119 34.8038295432592 38.0 35.2 38.0 25.6 38.0 120-124 34.34105183126328 37.6 34.6 38.0 24.4 38.0 125-129 33.9204670235506 37.0 34.0 38.0 23.0 38.0 130-134 33.79415503885631 37.0 34.0 38.0 23.0 38.0 135-139 33.173889307815514 37.0 32.0 38.0 21.6 38.0 140-144 31.902505250431563 36.2 28.4 38.0 17.4 38.0 145-149 31.820137975732724 35.6 28.4 37.6 18.6 38.0 150-154 30.82496525678817 35.0 27.0 37.2 14.2 38.0 155-159 30.664758854066047 35.0 26.2 37.0 15.0 37.6 160-164 29.97734804908297 35.0 24.6 37.0 11.0 37.4 165-169 28.860615842904593 33.4 23.2 36.6 10.0 37.0 170-174 28.238511681820732 32.6 22.2 36.2 9.6 37.0 175-179 27.41497728448303 30.8 20.4 36.2 9.2 37.0 180-184 26.523056405526507 28.8 20.0 35.2 9.0 37.0 185-189 25.797315892137128 28.0 19.8 35.0 9.0 36.8 190-194 24.751862423113117 26.0 17.0 34.8 8.6 36.0 195-199 23.87621881679889 25.0 15.4 34.0 8.0 36.0 200-204 23.00999794345114 23.8 14.8 32.4 8.0 36.0 205-209 22.09200563369749 22.8 12.4 31.4 8.0 35.8 210-214 20.7861581797673 20.4 9.4 29.4 8.0 35.0 215-219 20.210805606276836 20.0 9.0 28.2 8.0 34.4 220-224 19.481988994347606 19.4 9.0 27.2 8.0 34.0 225-229 18.636935617556695 17.8 9.0 25.8 8.0 32.8 230-234 17.682178446121533 16.2 9.0 24.8 7.8 31.4 235-239 16.705640552650767 14.8 8.6 23.4 7.0 30.2 240-244 15.808916074110545 13.8 8.0 22.0 7.0 28.8 245-249 15.091876008799536 13.0 8.0 20.6 7.0 27.4 250-254 14.366621588777477 12.2 8.0 19.6 7.0 26.0 255-259 13.728769872182372 11.2 8.0 18.2 7.0 25.0 260-264 13.118462200008725 10.6 7.8 17.2 7.0 23.8 265-269 12.68153032163178 9.8 7.2 16.0 7.0 22.6 270-274 12.177810460978543 9.0 7.0 14.6 7.0 21.4 275-279 11.686752086150701 8.2 7.0 14.0 7.0 20.6 280-284 11.514643874288776 8.0 7.0 13.6 7.0 20.2 285-289 11.196207225341666 8.0 7.0 13.0 7.0 19.0 290-294 10.934386120164774 8.0 7.0 13.0 7.0 19.0 295-299 10.65724247957473 8.0 7.0 13.0 7.0 18.4 300-301 10.28250593594785 8.0 7.0 12.5 7.0 16.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.07632066652833203 1101 2 0.12202840108700741 1101 3 0.2352982137420554 1101 4 0.15915799485078708 1101 5 0.13723541271726702 1101 6 0.18079587097292205 1101 7 1.0100995059960667 1101 8 0.2951345824742404 1101 9 0.20961128900426473 1101 10-14 0.22766371643765382 1101 15-19 0.30189543736799607 1101 20-24 0.2345819951471242 1101 25-29 0.18583817479287745 1101 30-34 0.5809980871294869 1101 35-39 0.2718281777349745 1101 40-44 0.4753959582631211 1101 45-49 0.27458313755726493 1101 50-54 0.3212813211798746 1101 55-59 0.4559585766502181 1101 60-64 0.39748489545100085 1101 65-69 0.3835351690545963 1101 70-74 0.35843447299323117 1101 75-79 0.410659252168017 1101 80-84 0.8890686653042223 1101 85-89 0.448495650733328 1101 90-94 0.606395265763922 1101 95-99 1.1169691415509675 1101 100-104 0.5198296548580856 1101 105-109 0.6768357353232872 1101 110-114 0.6801826086279235 1101 115-119 0.7105645648763073 1101 120-124 1.0440882039065045 1101 125-129 1.0742706279374303 1101 130-134 1.1235187275505396 1101 135-139 0.8552543487908011 1101 140-144 1.5794334825282235 1101 145-149 1.4744239049767316 1101 150-154 1.5042629631957354 1101 155-159 1.0195418868161354 1101 160-164 1.4845785092922092 1101 165-169 0.6760960100127527 1101 170-174 0.7676202640916152 1101 175-179 0.8828063469645535 1101 180-184 0.7797006442540848 1101 185-189 0.7270790618225433 1101 190-194 0.915602889343635 1101 195-199 0.9401126845952525 1101 200-204 0.952660347676094 1101 205-209 0.6289464407637375 1101 210-214 0.8485087700840133 1101 215-219 0.5154966837819863 1101 220-224 0.7437393335930871 1101 225-229 0.44784876481177704 1101 230-234 0.28085175661878736 1101 235-239 0.020138591176113607 1101 240-244 0.0428651818960315 1101 245-249 0.011774447917646924 1101 250-254 0.05618581297006209 1101 255-259 0.04989441145033524 1101 260-264 -0.06887561671533149 1101 265-269 -0.02034688398607365 1101 270-274 -0.15569457854727276 1101 275-279 0.10992724946746435 1101 280-284 -0.024909590196088516 1101 285-289 -0.08894300712890946 1101 290-294 -0.15833458639415987 1101 295-299 -0.16534355644916765 1101 300-301 -0.27597989263539624 1102 1 0.11643390375284213 1102 2 0.1628921440402351 1102 3 0.1718071543931572 1102 4 0.012360799614342 1102 5 0.020838012745159062 1102 6 0.18358366907250456 1102 7 0.8093420990652476 1102 8 0.2945623577998546 1102 9 0.29461253265839105 1102 10-14 0.11421600526446696 1102 15-19 0.1433270917442897 1102 20-24 0.19084591436855902 1102 25-29 0.14570768456311356 1102 30-34 0.38965075681639405 1102 35-39 0.18648302417604157 1102 40-44 0.4305059389828898 1102 45-49 0.14894330266852762 1102 50-54 -0.009226449142374804 1102 55-59 0.2118429297559814 1102 60-64 0.19713034619295655 1102 65-69 0.2478920417173498 1102 70-74 0.08375771523548536 1102 75-79 0.17767783221692923 1102 80-84 0.7368014047507785 1102 85-89 0.2755949460918359 1102 90-94 0.40656808336658656 1102 95-99 0.9205169345321522 1102 100-104 0.8020005429996644 1102 105-109 0.7212498592084842 1102 110-114 0.5898844048095384 1102 115-119 0.5444506174030863 1102 120-124 0.8601737558085745 1102 125-129 0.7786446960954194 1102 130-134 0.8558478401278364 1102 135-139 1.139572085886634 1102 140-144 1.6979734182032686 1102 145-149 1.7181522219029404 1102 150-154 1.4333321204295615 1102 155-159 0.6522093020715296 1102 160-164 1.213775913865124 1102 165-169 1.3700199328860663 1102 170-174 1.419050322564928 1102 175-179 1.404335653350607 1102 180-184 1.1835236226691386 1102 185-189 1.3648473280824263 1102 190-194 1.9533927218600873 1102 195-199 1.3424697326635595 1102 200-204 1.4844244243311984 1102 205-209 1.3140793823580026 1102 210-214 1.4463855002369428 1102 215-219 0.9819058014201651 1102 220-224 1.0599834693684898 1102 225-229 1.3601710049419786 1102 230-234 1.0527129676934912 1102 235-239 0.6850374957540524 1102 240-244 1.0147016636631978 1102 245-249 0.6161178919913102 1102 250-254 0.5111259953630825 1102 255-259 0.4699843858727544 1102 260-264 0.6022550733617464 1102 265-269 0.36276558691745286 1102 270-274 0.38596495927347263 1102 275-279 0.1933955636044331 1102 280-284 0.41307462617960056 1102 285-289 0.30714372372852417 1102 290-294 0.035639226419361236 1102 295-299 0.054601492729085876 1102 300-301 0.1455908209632799 1103 1 -0.013294341659893405 1103 2 0.11978996392313945 1103 3 0.0689741650086404 1103 4 0.12330285509727901 1103 5 0.08019194938292173 1103 6 -0.20002223143592346 1103 7 0.8413902827213064 1103 8 0.19251393597150468 1103 9 0.09552851528022188 1103 10-14 0.03786805515735381 1103 15-19 0.126793171106101 1103 20-24 0.048665461028612356 1103 25-29 0.03158728986958437 1103 30-34 0.338929865323486 1103 35-39 0.03825383518072556 1103 40-44 0.35663918377216675 1103 45-49 -0.027365839364819067 1103 50-54 0.0553812880947433 1103 55-59 0.22090412462434017 1103 60-64 0.2164937536819309 1103 65-69 0.1886291709566379 1103 70-74 0.16353954941433813 1103 75-79 0.19169469201330003 1103 80-84 0.728122126160649 1103 85-89 0.3875157496010999 1103 90-94 0.28844499312310745 1103 95-99 1.0085017027134597 1103 100-104 0.5277933415100975 1103 105-109 0.6200616596649411 1103 110-114 0.44060753792404483 1103 115-119 0.564500241301019 1103 120-124 0.8332440348484411 1103 125-129 0.7476301801946263 1103 130-134 0.8810775713153731 1103 135-139 0.8349306682851321 1103 140-144 1.5622056838242173 1103 145-149 1.4660247128628967 1103 150-154 1.7796214661422454 1103 155-159 1.0497174594725927 1103 160-164 0.8620579210691375 1103 165-169 1.3812081464397536 1103 170-174 1.7273901355856687 1103 175-179 1.445727195992557 1103 180-184 1.4473285791746733 1103 185-189 1.6061943184952838 1103 190-194 1.4982729282013558 1103 195-199 1.518534537943701 1103 200-204 1.4928330271144503 1103 205-209 1.3897066511242286 1103 210-214 2.076188699871558 1103 215-219 2.019983386745391 1103 220-224 1.4871933930272618 1103 225-229 1.1333617711422477 1103 230-234 1.677831458409674 1103 235-239 1.3629926885405155 1103 240-244 0.7233351291121028 1103 245-249 1.0634334800877419 1103 250-254 0.853955958558462 1103 255-259 0.8604866351999192 1103 260-264 0.7247966799599599 1103 265-269 0.7765863048247876 1103 270-274 0.7508342657297593 1103 275-279 0.5196955289993994 1103 280-284 0.5284985238271602 1103 285-289 0.4993229862069999 1103 290-294 0.24871600616182477 1103 295-299 0.349574236011577 1103 300-301 -0.011125847874959405 2116 1 -0.021038195820523242 2116 2 0.10568301762802434 2116 3 0.19348138055924835 2116 4 0.11771911415637248 2116 5 0.15665112931235115 2116 6 0.20645700471714434 2116 7 0.8020571866417896 2116 8 0.2506480174819856 2116 9 0.17702244185844052 2116 10-14 0.07794215559296447 2116 15-19 0.09238943684140821 2116 20-24 0.17450736722507543 2116 25-29 0.15232327327285589 2116 30-34 0.49832359906401535 2116 35-39 0.08119318924342167 2116 40-44 0.535828437829629 2116 45-49 0.25459100802856227 2116 50-54 0.1903042219864517 2116 55-59 0.42544974218775167 2116 60-64 0.26716206457270886 2116 65-69 0.33476372697474943 2116 70-74 0.3629144132002935 2116 75-79 0.40553155518681905 2116 80-84 0.805991311028265 2116 85-89 0.40044387548908134 2116 90-94 0.6005722494482626 2116 95-99 1.1763962958093686 2116 100-104 0.5543238355221973 2116 105-109 0.4836459578764334 2116 110-114 0.6364975293994561 2116 115-119 0.731862116820011 2116 120-124 0.9806672101321041 2116 125-129 0.9291663158817371 2116 130-134 0.9244566725648227 2116 135-139 1.2765728509098722 2116 140-144 1.8664395609101945 2116 145-149 1.9119592583976939 2116 150-154 2.1320782845685926 2116 155-159 1.8700354899348781 2116 160-164 1.8166789908631387 2116 165-169 1.5247196684229785 2116 170-174 1.6750422883881626 2116 175-179 2.004131360624637 2116 180-184 2.059925377351682 2116 185-189 1.4837382449355694 2116 190-194 1.6308067713545142 2116 195-199 0.9073262263356412 2116 200-204 0.9184920271723875 2116 205-209 0.8033495730980746 2116 210-214 1.809753493612476 2116 215-219 1.9615822567551326 2116 220-224 1.3260078412328227 2116 225-229 0.6525820888968319 2116 230-234 1.4716096150578828 2116 235-239 0.8045799717360502 2116 240-244 0.7176683493360638 2116 245-249 0.9970711052322336 2116 250-254 0.9471078754865196 2116 255-259 1.4979272182925474 2116 260-264 0.6055065654436138 2116 265-269 0.6406269028147502 2116 270-274 0.4371667907448664 2116 275-279 0.6761997319783148 2116 280-284 0.31213711453949955 2116 285-289 0.1993149857876677 2116 290-294 0.18859975179263344 2116 295-299 0.3305230305690863 2116 300-301 0.15276756998694374 1117 1 0.1532724819030804 1117 2 0.07711416874413146 1117 3 0.0706368583272976 1117 4 0.11694957351934931 1117 5 0.06026408093130442 1117 6 0.11050612196791576 1117 7 1.0324207043037177 1117 8 0.34958955184044527 1117 9 0.3811368450477346 1117 10-14 0.20651406041440623 1117 15-19 0.23504754688294582 1117 20-24 0.2608878870122737 1117 25-29 0.3028312534370414 1117 30-34 0.573984167746211 1117 35-39 0.34354959490524095 1117 40-44 0.6409890829102594 1117 45-49 0.2398330521729619 1117 50-54 0.4338257732914741 1117 55-59 0.4734965813759757 1117 60-64 0.43611679502512857 1117 65-69 0.4678825890547387 1117 70-74 0.5397809659638355 1117 75-79 0.627158147399733 1117 80-84 0.9210304131382472 1117 85-89 0.5203266386122394 1117 90-94 0.552419563695878 1117 95-99 1.1353717232933604 1117 100-104 0.45242443143001765 1117 105-109 0.7290569120234807 1117 110-114 0.9233306057562274 1117 115-119 1.0073757226874136 1117 120-124 1.081356185142866 1117 125-129 1.1399059916745387 1117 130-134 1.2836107334891054 1117 135-139 1.3404016628447053 1117 140-144 1.534578078224861 1117 145-149 2.052387367466885 1117 150-154 2.4991666706168445 1117 155-159 1.9998342147754897 1117 160-164 2.341599050315015 1117 165-169 1.8400952014757195 1117 170-174 1.500091808467058 1117 175-179 2.152471292004943 1117 180-184 2.133535967564349 1117 185-189 2.0268500227966904 1117 190-194 -0.4878175011239918 1117 195-199 1.9310291892060008 1117 200-204 2.413979796476884 1117 205-209 2.3497881556986755 1117 210-214 2.82218865911927 1117 215-219 2.1786018842085646 1117 220-224 1.8784455070521595 1117 225-229 2.080194707140784 1117 230-234 1.918469639227574 1117 235-239 1.3223585829513382 1117 240-244 1.5438196738071728 1117 245-249 1.5428140453219648 1117 250-254 1.3300657452202351 1117 255-259 1.367827344697238 1117 260-264 0.947840468292581 1117 265-269 0.5296877576438277 1117 270-274 0.7286829524873042 1117 275-279 0.467830908097552 1117 280-284 0.49847957401360077 1117 285-289 0.6070459521584635 1117 290-294 0.3612363313650775 1117 295-299 0.22401888421745753 1117 300-301 -0.02623883359128243 2117 1 0.15466725627580757 2117 2 0.03150695740579579 2117 3 0.08447132781490296 2117 4 0.11440977400110341 2117 5 -0.023688507658022218 2117 6 0.19671191260163567 2117 7 0.958722846278647 2117 8 0.3362709578057661 2117 9 0.1773605821423132 2117 10-14 0.12755481866942375 2117 15-19 0.2752626314633204 2117 20-24 0.24893786415270824 2117 25-29 0.1572299105231707 2117 30-34 0.5176707665847999 2117 35-39 0.25398924907761966 2117 40-44 0.5971052777146539 2117 45-49 0.203871074104498 2117 50-54 0.2775283397036148 2117 55-59 0.4831106983486819 2117 60-64 0.28687702894325184 2117 65-69 0.3429361896060925 2117 70-74 0.3115647622885689 2117 75-79 0.4352740419591612 2117 80-84 0.7379522197941384 2117 85-89 0.3292919534941561 2117 90-94 0.2901550342275243 2117 95-99 1.0798932761057785 2117 100-104 0.5341407408734113 2117 105-109 0.47170766570659595 2117 110-114 0.6884707996871811 2117 115-119 0.6592171114568828 2117 120-124 0.8947266396728182 2117 125-129 0.9133990007433042 2117 130-134 0.6163291105285538 2117 135-139 1.354788856031881 2117 140-144 2.0598940519397715 2117 145-149 1.8420185438130403 2117 150-154 1.971617693155693 2117 155-159 1.747772555376116 2117 160-164 1.6273692127884836 2117 165-169 1.6684492448884107 2117 170-174 1.5315596651956156 2117 175-179 2.0785285978522197 2117 180-184 1.5303369938562597 2117 185-189 1.662950932402481 2117 190-194 1.751320522854666 2117 195-199 2.0098475887638223 2117 200-204 2.253922476982112 2117 205-209 1.7496986281068416 2117 210-214 2.0765231729612488 2117 215-219 2.262316187469633 2117 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2110 110-114 -0.4659262830221991 2110 115-119 -0.686499461139924 2110 120-124 -0.40368976306097437 2110 125-129 -0.6829204960323239 2110 130-134 -1.1760806437142364 2110 135-139 -1.3012976267014764 2110 140-144 -0.13978220264259988 2110 145-149 -0.437406814958873 2110 150-154 -1.0963021667658666 2110 155-159 -1.352152363239803 2110 160-164 -1.1035250547133977 2110 165-169 -1.7771393449454322 2110 170-174 -1.7351759753174711 2110 175-179 -1.476955442876335 2110 180-184 -1.5330920349488224 2110 185-189 -1.352080222927814 2110 190-194 -1.3935614753847148 2110 195-199 -1.7983562578827907 2110 200-204 -1.3592474866068613 2110 205-209 -1.5268270738352179 2110 210-214 -1.2506079329117732 2110 215-219 -1.025143843747692 2110 220-224 -1.3105854726008275 2110 225-229 -1.3476318841307808 2110 230-234 -1.1354627123124317 2110 235-239 -1.4256712712986097 2110 240-244 -0.8268419665195363 2110 245-249 -0.9141766407596297 2110 250-254 -0.7160884833263363 2110 255-259 -0.8890473489238655 2110 260-264 -0.6344646056871337 2110 265-269 -0.5785053145683836 2110 270-274 -0.43624783177362225 2110 275-279 -0.41096553203860076 2110 280-284 -0.42107385501351224 2110 285-289 -0.3840607401922469 2110 290-294 -0.44273061078368237 2110 295-299 -0.21416003415284735 2110 300-301 -0.5238968905794827 2109 1 0.13673211718457878 2109 2 0.0468501457675643 2109 3 0.11882537696116202 2109 4 0.09905968113552888 2109 5 0.11320708933990176 2109 6 -0.11480963290249946 2109 7 0.9611725642296634 2109 8 0.15558975946008502 2109 9 0.07010878179294622 2109 10-14 -0.01580505097353324 2109 15-19 0.022595904957618984 2109 20-24 -0.07188625742179511 2109 25-29 -0.07598323841389032 2109 30-34 -3.902580419263721 2109 35-39 -0.05081007151920147 2109 40-44 0.26139394120985315 2109 45-49 -0.3039573904028998 2109 50-54 -0.326251738041087 2109 55-59 -0.006025893450143371 2109 60-64 0.06699537213852125 2109 65-69 -0.0862057877057083 2109 70-74 -0.014885977529011996 2109 75-79 -0.28216328240752375 2109 80-84 0.0461074054217292 2109 85-89 -0.1324738043096616 2109 90-94 0.08520478117745256 2109 95-99 0.2675908312209714 2109 100-104 -0.19840916142786114 2109 105-109 -0.03172235369505927 2109 110-114 -0.36522055518359764 2109 115-119 -0.3868732132619286 2109 120-124 -0.07614961090543915 2109 125-129 -0.5642567821238558 2109 130-134 -0.5889315690127148 2109 135-139 -1.0890055830807555 2109 140-144 -0.3271721553472382 2109 145-149 -0.6017778285606568 2109 150-154 -0.5472861478197792 2109 155-159 -1.6445631692170402 2109 160-164 -1.6076614777309395 2109 165-169 -1.1789545926348062 2109 170-174 -1.2156948969552772 2109 175-179 -1.4105530340963028 2109 180-184 -1.1861000059987497 2109 185-189 -0.9334960099380041 2109 190-194 -1.1757541079269949 2109 195-199 -1.8724463635812896 2109 200-204 -1.4789839738957937 2109 205-209 -1.698988618584174 2109 210-214 -1.6247029288249486 2109 215-219 -0.8897008252277523 2109 220-224 -1.010651001477548 2109 225-229 -1.2328957112127945 2109 230-234 -1.215673410704948 2109 235-239 -1.3554835569150896 2109 240-244 -0.9103081506850224 2109 245-249 -1.0904272433039388 2109 250-254 -0.7775269050444784 2109 255-259 -0.8787597344404485 2109 260-264 -0.8969707708506434 2109 265-269 -0.7078263431901117 2109 270-274 -0.49347144731891746 2109 275-279 -0.3265190694103204 2109 280-284 -0.7179431395751052 2109 285-289 -1.0877282614821464 2109 290-294 -0.5313080473331464 2109 295-299 -0.4992615061074659 2109 300-301 -0.3907600973028291 2108 1 -0.07205520513059582 2108 2 -0.20350692421815353 2108 3 -0.21508606968237842 2108 4 0.018300652365887515 2108 5 0.01186722672682805 2108 6 -0.18594477539042487 2108 7 0.8804747474575763 2108 8 0.1184340769463148 2108 9 -0.21630215475061476 2108 10-14 -0.1461417169600665 2108 15-19 -0.06428296330055616 2108 20-24 -0.01923713819442696 2108 25-29 -0.11102234607898254 2108 30-34 0.19633544824630178 2108 35-39 -0.23466778763156526 2108 40-44 -0.07471569627908536 2108 45-49 -0.14849523982137924 2108 50-54 -0.2267509558877947 2108 55-59 -0.03352096248367076 2108 60-64 -0.20369768370041896 2108 65-69 -0.4072817585960067 2108 70-74 -0.3940806996945625 2108 75-79 -0.3758706890592194 2108 80-84 -0.22429727389542364 2108 85-89 -0.4338980034526898 2108 90-94 -0.2725290146465227 2108 95-99 -0.260591172451754 2108 100-104 -0.7670795008143827 2108 105-109 -0.76923306579463 2108 110-114 -1.027889398276848 2108 115-119 -0.7070949367197272 2108 120-124 -0.5581396809591723 2108 125-129 -0.5668453694869768 2108 130-134 -0.6592083832683073 2108 135-139 -1.1790538725454915 2108 140-144 -0.541550173438786 2108 145-149 -1.132262763607926 2108 150-154 -0.8948805163160003 2108 155-159 -2.0603109757871287 2108 160-164 -1.7091047206065433 2108 165-169 -1.3770937479092353 2108 170-174 -2.227195951161896 2108 175-179 -1.6232375202555893 2108 180-184 -2.0556150709514824 2108 185-189 -1.760002165144428 2108 190-194 -1.4107374446609953 2108 195-199 -1.5520682094630835 2108 200-204 -1.6298919518594772 2108 205-209 -1.271103153032925 2108 210-214 -1.2807595159160172 2108 215-219 -1.308899628193135 2108 220-224 -0.7103707865554192 2108 225-229 -1.4498718385337455 2108 230-234 -0.8360978138879744 2108 235-239 -1.1730840466110735 2108 240-244 -0.721229731314823 2108 245-249 -1.2551936175132834 2108 250-254 -0.9838725487906537 2108 255-259 -0.4863690658694093 2108 260-264 -0.26147189307059726 2108 265-269 -0.36680750349753133 2108 270-274 -0.46524727538121624 2108 275-279 -0.21672242926885232 2108 280-284 -0.6365597484304377 2108 285-289 -1.07211920210651 2108 290-294 -0.7840510249567618 2108 295-299 -0.5512019264298491 2108 300-301 2.5315550764712302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 165.0 11 609.0 12 706.0 13 580.0 14 642.0 15 998.0 16 1434.0 17 2070.0 18 2699.0 19 3095.0 20 4124.0 21 5571.0 22 7792.0 23 10666.0 24 15405.0 25 22156.0 26 31657.0 27 43487.0 28 52839.0 29 52195.0 30 38602.0 31 18135.0 32 4703.0 33 572.0 34 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.39119921726504 22.777525036924402 14.48527074777363 33.34600499803693 2 44.4703763484417 23.801437091416712 17.466331802346957 14.261854757794632 3 43.68140942148657 22.92366464543228 18.825212042651575 14.56971389042957 4 43.91479655746184 21.853947639019587 19.065765939811673 15.165489863706899 5 28.26196693318709 22.039037036575408 35.088151162573304 14.610844867664197 6 27.33122277409746 21.39496332487863 35.49354056698429 15.780273334039624 7 16.85933828982382 47.258246449337236 20.41249384593333 15.469921414905615 8 26.661286402472843 20.43524052273733 36.64271514305478 16.260757931735043 9 15.588640371923745 19.255840910365627 37.73704841614578 27.418470301564845 10-14 26.213706586565127 34.01039491970111 23.7225403987212 16.053358095012555 15-19 21.305721568211986 34.96930756623022 22.046889314047473 21.678081551510317 20-24 25.014676280513264 30.78354511631965 26.555592254912348 17.646186348254737 25-29 19.288870331478282 25.69614178969607 37.46383901584789 17.551148862977758 30-34 19.61305721568212 28.269196014034385 31.202457887488084 20.91528888279541 35-39 20.60069922661299 29.699494587537313 27.898643300947885 21.801162884901814 40-44 20.106317344185264 28.837551335821964 29.51702261580551 21.539108704187257 45-49 21.06161545029072 30.151000542181063 26.36987966073176 22.417504346796456 50-54 22.036668889401295 29.504683322635124 27.77693300013087 20.681714787832707 55-59 21.489938490493135 26.911125929341967 30.071356013535834 21.52757956662907 60-64 22.096558085041412 25.851068470613164 29.352249428217096 22.700124016128328 65-69 20.100770894224837 26.79159681671164 29.259081532814417 23.848550756249104 70-74 20.83221677271396 25.937318883480927 29.671326100097843 23.559138243707274 75-79 20.215376753519504 25.89294728379751 30.669500133987277 23.22217582869571 80-84 23.000193190953677 25.70037952674448 27.618952655752416 23.680474626549422 85-89 21.158647164766954 27.514442581778976 26.17675102671644 25.150159226737628 90-94 20.167452933074912 29.860216997064743 28.957579005752105 21.014751064108236 95-99 22.157070477306295 29.59710338208808 26.88096321270324 21.364862927902383 100-104 22.57536005184996 28.2386593794208 26.820637779425788 22.36534278930345 105-109 23.451200588297613 27.492069822949837 24.062307198544215 24.994422390208335 110-114 21.462704797990813 27.83096414749818 29.366832229236646 21.33949882527436 115-119 23.38994036008301 27.838130908683002 27.062001832198078 21.709926899035914 120-124 18.81891775674143 28.5460822744184 27.944012015230925 24.69098795360924 125-129 20.614534191682818 24.89209350442158 30.885811682443926 23.607560621451672 130-134 24.937212940054717 28.578737777556196 26.205355751793245 20.278693530595838 135-139 24.07389865576488 27.370733440107685 24.979527990876402 23.57583991325103 140-144 21.172482129836784 28.751487881941628 26.785177891476543 23.290852096745045 145-149 21.17802857979721 26.762368895010063 29.09050684581492 22.9690956793778 150-154 23.188086973320953 25.47197796376735 25.786567619949768 25.553367442961928 155-159 22.140057209450152 26.651190617151617 29.83722104161084 21.371531131787393 160-164 24.8318303908066 29.03211332207425 26.077475804391042 20.058580482728104 165-169 20.703651309024508 25.466369194144445 28.27704829150645 25.55293120532459 170-174 22.77621632401239 28.831381689236775 26.182733714314203 22.209668272436637 175-179 20.894100197553332 28.966989274786087 28.247321812505067 21.891588715155518 180-184 20.630924262914192 29.16666147336146 26.607379894430494 23.595034369293856 185-189 22.92061098197092 27.25500582688844 27.597327732873 22.22705545826764 190-194 21.423568049955442 27.694982643973997 26.023133058711355 24.858316247359205 195-199 22.282146039897047 29.358356755139813 26.999744489383847 21.35975271557929 200-204 23.484728566710082 27.536441422633256 26.884141515489553 22.094688495167112 205-209 23.02437321999464 27.166761184821425 27.359640540186835 22.4492250549971 210-214 24.451119572736392 27.261860989760876 26.78760835831313 21.499411079189596 215-219 24.884490505599423 26.83073356474701 27.11852576606445 21.166250163589115 220-224 21.98145366844693 26.938421941506764 27.469634744458226 23.61048964558808 225-229 24.18295806509912 28.069399176134063 25.725307391735164 22.022335367031655 230-234 24.386369443423096 26.32419934813633 27.301309336108638 21.988121872331938 235-239 24.732804447131116 27.094844294323302 24.669549989717254 23.50280126882833 240-244 25.766251409982367 30.577703271159084 22.477206583449146 21.178838735409407 245-249 24.409988595501765 29.28650218430417 22.98062481693599 23.322884403258072 250-254 25.40492200694241 27.3693624075332 23.679165913637412 23.546549671886975 255-259 25.44312396004063 27.776621401818485 25.292061098197095 21.488193539943786 260-264 25.20730635722877 28.859986414313582 24.486267862373257 21.446439366084395 265-269 24.606918728928164 28.646105332693516 24.780105070950935 21.96687086742738 270-274 24.239855916940353 30.229523316901712 22.84134037130055 22.68928039485738 275-279 25.18480895907468 29.450714494930295 22.399182366028306 22.965294179966723 280-284 23.109065641300486 30.159039778640555 24.55326149953572 22.178633080523234 285-289 25.00981534684008 29.344521790069983 24.575135701065044 21.07052716202489 290-294 24.439216517203345 31.035565831375457 22.763565432529617 21.761652218891584 295-299 23.851355141060555 32.18530128440824 20.86854913593788 23.094794438593322 300-301 20.68607716420608 34.522288627284794 26.502371263157237 18.28926294535189 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.25 26 0.25 27 0.75 28 1.25 29 1.25 30 1.0 31 1.75 32 3.75 33 7.25 34 25.25 35 91.0 36 343.5 37 925.0 38 1691.5 39 2559.25 40 3730.0 41 5372.0 42 7642.0 43 10717.25 44 13216.0 45 13324.5 46 11521.25 47 9277.75 48 7592.5 49 6193.75 50 4916.25 51 3646.25 52 2051.75 53 892.0 54 357.0 55 166.25 56 115.0 57 89.0 58 77.0 59 71.75 60 67.0 61 67.75 62 66.0 63 52.0 64 41.25 65 29.25 66 19.5 67 12.25 68 9.5 69 6.5 70 3.25 71 5.75 72 4.5 73 3.5 74 1.0 75 2.75 76 3.25 77 1.5 78 1.25 79 1.75 80 1.0 81 1.5 82 0.75 83 1.0 84 0.75 85 0.75 86 0.75 87 0.5 88 0.0 89 0.5 90 0.5 91 0.75 92 0.5 93 0.75 94 0.25 95 0.75 96 0.25 97 0.0 98 0.0 99 0.25 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 320926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.604943184084703 #Duplication Level Percentage of deduplicated Percentage of total 1 47.93721930108382 7.001203642953378 2 5.328253475788091 1.5563767856857382 3 5.036091250292035 2.206554797411438 4 4.527716650011632 2.645081777082168 5 4.480835665826558 3.2721175158308613 6 4.763063788720387 4.173856560985948 7 3.8872451157005883 3.974109584019251 8 3.6810803227963187 4.300957517639393 9 3.104995129072771 4.081344970227081 >10 16.92325051959047 40.34366749166894 >50 0.24375483658541416 2.2946438766477137 >100 0.03776186912315324 1.1809668688193597 >500 0.015505660357501136 1.691003858943637 >1k 0.026581132040985146 12.386676057103854 >5k 0.004430188673497524 5.037101603873459 >10k+ 0.002215094336748762 3.8543370911077965 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 11914 3.712382293737497 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 8086 2.5195839539333056 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 7484 2.3320017698784143 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG 4925 1.5346216884889352 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 4900 1.5268317306793466 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 4709 1.4673164530140903 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 4467 1.3919096614172737 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 3488 1.086854913593788 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 3266 1.0176800882446422 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 2515 0.7836697556446034 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA 2162 0.6736755513732138 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 2130 0.6637044053769404 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 1998 0.6225734281423132 No Hit GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA 1976 0.6157182652698754 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 1752 0.5459202432959622 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT 984 0.3066127393854035 No Hit GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 970 0.30225036301203395 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 771 0.24024229884770945 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 728 0.22684357141521724 No Hit GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA 645 0.2009809114873834 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 613 0.1910097654911101 Illumina Single End PCR Primer 1 (97% over 34bp) GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA 516 0.16078472918990672 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 496 0.15455276294223588 No Hit GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG 491 0.1529947713803182 No Hit GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA 321 0.10002305827511639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 3.115983123835401E-4 0.0 0.0 8 6.231966247670802E-4 0.0 0.0 9 9.347949371506204E-4 0.0 0.0 10-14 0.0013087129120108684 0.0 0.0 15-19 0.0019319095367779488 0.0 0.0 20-24 0.002492786499068321 0.0 1.8695898743012406E-4 25-29 0.002492786499068321 0.0 3.115983123835401E-4 30-34 0.003801499411079189 0.0 3.115983123835401E-4 35-39 0.0051102123230900584 0.0 3.115983123835401E-4 40-44 0.006418925235100926 0.0 3.115983123835401E-4 45-49 0.008787072409215831 0.0 3.115983123835401E-4 50-54 0.012152334182958066 0.0 3.115983123835401E-4 55-59 0.016639349881281046 0.0 3.115983123835401E-4 60-64 0.024429307690869546 0.0 3.115983123835401E-4 65-69 0.03383957672485246 0.0 3.115983123835401E-4 70-74 0.04742526314477481 0.0 3.115983123835401E-4 75-79 0.07116905454840057 0.0 3.115983123835401E-4 80-84 0.10451007397343934 0.0 3.115983123835401E-4 85-89 0.1477599197322747 0.0 3.115983123835401E-4 90-94 0.2044084929236023 0.0 3.115983123835401E-4 95-99 0.27296012164798117 0.0 3.115983123835401E-4 100-104 0.368620803549728 0.0 3.115983123835401E-4 105-109 0.48459769541888154 0.0 3.115983123835401E-4 110-114 0.6269981241781595 0.0 3.115983123835401E-4 115-119 0.7988757532889201 0.0 3.115983123835401E-4 120-124 1.0036581641873827 0.0 3.115983123835401E-4 125-129 1.248387478733415 0.0 3.115983123835401E-4 130-134 1.533562254226831 0.0 3.115983123835401E-4 135-139 1.826651626854789 0.0 3.115983123835401E-4 140-144 2.1732112698877626 0.0 3.115983123835401E-4 145-149 2.572804945688414 0.0 3.115983123835401E-4 150-154 3.0469329378112087 0.0 3.115983123835401E-4 155-159 3.5884908047338016 0.0 3.115983123835401E-4 160-164 4.170494132603778 0.0 3.115983123835401E-4 165-169 4.818493983036587 0.0 3.115983123835401E-4 170-174 5.546263001439584 0.0 3.115983123835401E-4 175-179 6.305752728043226 0.0 3.115983123835401E-4 180-184 7.124820051974598 0.0 3.115983123835401E-4 185-189 8.042601721269076 0.0 3.115983123835401E-4 190-194 9.021705938440638 0.0 3.115983123835401E-4 195-199 9.973015586147586 0.0 3.115983123835401E-4 200-204 10.903136548612453 0.0 3.115983123835401E-4 205-209 11.810261555623416 0.0 3.115983123835401E-4 210-214 12.702679122289874 0.0 3.115983123835401E-4 215-219 13.628250749393938 0.0 3.115983123835401E-4 220-224 14.56341960451942 0.0 3.115983123835401E-4 225-229 15.437639829742682 0.0 3.115983123835401E-4 230-234 16.225921240410564 0.0 3.115983123835401E-4 235-239 16.93094358200956 0.0 3.115983123835401E-4 240-244 17.539058848457277 0.0 3.115983123835401E-4 245-249 18.048958326841703 0.0 3.115983123835401E-4 250-254 18.479213276580897 0.0 3.115983123835401E-4 255-259 18.821784461215355 0.0 3.115983123835401E-4 260-264 19.11094769510728 0.0 3.115983123835401E-4 265-269 19.343711634457787 0.0 3.115983123835401E-4 270-274 19.522132828128605 0.0 3.115983123835401E-4 275-279 19.640290908184443 0.0 3.115983123835401E-4 280-284 19.724671731177903 0.0 3.115983123835401E-4 285-289 19.787053713317086 0.0 5.608769622903723E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTTAT 4980 0.0 291.14963 2 CGGGTTA 4970 0.0 291.14188 1 GTTATTA 5040 0.0 287.68353 4 GGTTATT 5090 0.0 284.85757 3 TTATTAG 5135 0.0 280.92502 5 TTAGTAG 5260 0.0 277.89447 8 TATTAGT 5255 0.0 274.79068 6 GGGGAGC 3560 0.0 273.45505 3 TAGTAGA 5350 0.0 272.94394 9 ATTAGTA 5345 0.0 270.1637 7 GGAGCGA 2705 0.0 257.37524 5 GGGAGCG 2710 0.0 256.35608 4 CGAAAGC 2790 0.0 255.34946 9 GGGGGGA 3790 0.0 255.30344 1 AGCGAAA 2930 0.0 238.11433 7 GAGCGAA 2920 0.0 237.91953 6 GCGAAAG 3010 0.0 236.196 8 GGGGGAG 4465 0.0 218.02911 2 GGGAGCA 1240 0.0 210.54434 4 GCAAAAG 1630 0.0 149.30981 8 >>END_MODULE