FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30295_flu.3520000003b977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30295_flu.3520000003b977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320926
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC119143.712382293737497No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT80862.5195839539333056No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG74842.3320017698784143No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG49251.5346216884889352No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA49001.5268317306793466No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA47091.4673164530140903No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44671.3919096614172737No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT34881.086854913593788No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT32661.0176800882446422No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT25150.7836697556446034No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA21620.6736755513732138No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG21300.6637044053769404No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19980.6225734281423132No Hit
GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA19760.6157182652698754No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17520.5459202432959622No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9840.3066127393854035No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT9700.30225036301203395No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7710.24024229884770945No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7280.22684357141521724No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA6450.2009809114873834No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT6130.1910097654911101Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA5160.16078472918990672No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4960.15455276294223588No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4910.1529947713803182No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA3210.10002305827511639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT49800.0291.149632
CGGGTTA49700.0291.141881
GTTATTA50400.0287.683534
GGTTATT50900.0284.857573
TTATTAG51350.0280.925025
TTAGTAG52600.0277.894478
TATTAGT52550.0274.790686
GGGGAGC35600.0273.455053
TAGTAGA53500.0272.943949
ATTAGTA53450.0270.16377
GGAGCGA27050.0257.375245
GGGAGCG27100.0256.356084
CGAAAGC27900.0255.349469
GGGGGGA37900.0255.303441
AGCGAAA29300.0238.114337
GAGCGAA29200.0237.919536
GCGAAAG30100.0236.1968
GGGGGAG44650.0218.029112
GGGAGCA12400.0210.544344
GCAAAAG16300.0149.309818
Produced by FastQC (version 0.11.2)