##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30292_flu.3520000003b9ae.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297430
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31482029385065	34.0	34.0	34.0	34.0	34.0
2	33.498739199139294	34.0	34.0	34.0	34.0	34.0
3	33.520912483609585	34.0	34.0	34.0	34.0	34.0
4	33.5123760212487	34.0	34.0	34.0	34.0	34.0
5	33.46024947046364	34.0	34.0	34.0	34.0	34.0
6	36.99190061527082	38.0	38.0	38.0	36.0	38.0
7	36.11183807954813	38.0	38.0	38.0	34.0	38.0
8	36.894331439330266	38.0	38.0	38.0	36.0	38.0
9	36.97177150926268	38.0	38.0	38.0	36.0	38.0
10-14	36.99084893924621	38.0	38.0	38.0	36.4	38.0
15-19	36.912466126483544	38.0	38.0	38.0	36.6	38.0
20-24	37.01695256026628	38.0	38.0	38.0	36.6	38.0
25-29	36.944429949904176	38.0	38.0	38.0	36.0	38.0
30-34	36.6185408331372	38.0	38.0	38.0	34.6	38.0
35-39	36.89535487341559	38.0	38.0	38.0	36.0	38.0
40-44	36.43790135494066	38.0	37.6	38.0	34.2	38.0
45-49	36.748457788387185	38.0	38.0	38.0	35.2	38.0
50-54	36.722797969270076	38.0	38.0	38.0	35.4	38.0
55-59	36.39510271324345	38.0	37.4	38.0	34.2	38.0
60-64	36.568577480415556	38.0	37.6	38.0	34.2	38.0
65-69	36.44275291665266	38.0	37.6	38.0	34.0	38.0
70-74	36.31325353864774	38.0	37.0	38.0	34.0	38.0
75-79	36.36346098241603	38.0	37.0	38.0	34.0	38.0
80-84	35.7040016138251	38.0	37.0	38.0	30.6	38.0
85-89	35.91520895672931	38.0	37.0	38.0	32.0	38.0
90-94	35.913517802508146	38.0	37.0	38.0	32.0	38.0
95-99	35.013388696500016	38.0	36.0	38.0	27.2	38.0
100-104	35.370152304743975	38.0	36.2	38.0	28.8	38.0
105-109	35.211791009649325	38.0	35.8	38.0	28.0	38.0
110-114	34.74960831119928	38.0	35.2	38.0	24.6	38.0
115-119	34.81832565645698	38.0	35.2	38.0	25.6	38.0
120-124	34.357658608748274	37.6	34.6	38.0	24.4	38.0
125-129	33.93803247823017	37.0	34.0	38.0	23.0	38.0
130-134	33.79827589684968	37.0	34.0	38.0	23.0	38.0
135-139	33.191072185051944	37.0	31.8	38.0	22.0	38.0
140-144	31.89937867733584	36.2	28.4	38.0	17.4	38.0
145-149	31.800529872575062	35.6	28.2	37.6	18.4	38.0
150-154	30.804701610462967	35.0	26.8	37.2	14.2	38.0
155-159	30.668093332885046	35.0	26.2	37.0	15.0	37.6
160-164	29.993308005244934	35.0	24.6	37.0	11.0	37.4
165-169	28.89153078035168	33.8	23.4	36.6	10.0	37.0
170-174	28.272442591534144	32.8	22.4	36.2	9.6	37.0
175-179	27.464043304306898	31.0	20.4	36.2	9.2	37.0
180-184	26.582157146219277	28.8	20.0	35.2	9.0	37.0
185-189	25.869078438624218	28.0	19.8	35.0	9.0	36.8
190-194	24.87639444575194	26.2	17.6	34.8	8.6	36.0
195-199	23.980723531587266	25.0	15.4	34.2	8.0	36.0
200-204	23.12558047271627	24.4	15.0	32.4	8.0	36.0
205-209	22.241196920283762	23.2	12.4	31.6	8.0	35.8
210-214	20.960063208149815	21.0	9.6	29.4	8.0	35.0
215-219	20.449371616851025	20.4	9.0	28.6	8.0	34.6
220-224	19.750404464916116	20.0	9.0	27.8	8.0	34.0
225-229	18.9697501933228	18.4	9.0	26.4	8.0	33.2
230-234	18.02784991426554	16.6	9.0	25.0	8.0	31.8
235-239	17.079452644319673	15.4	9.0	23.8	8.0	30.8
240-244	16.184873751807153	14.2	8.0	22.4	7.0	29.2
245-249	15.510013112328952	13.6	8.0	21.2	7.0	28.2
250-254	14.780667047708704	12.8	8.0	20.2	7.0	26.8
255-259	14.144788353562182	12.0	8.0	19.2	7.0	25.2
260-264	13.550911474968899	11.4	8.0	18.2	7.0	24.4
265-269	13.140858689439531	10.6	8.0	17.2	7.0	23.6
270-274	12.627007363077027	9.4	8.0	16.0	7.0	22.2
275-279	12.072393504353967	9.0	7.4	14.6	7.0	21.2
280-284	11.94411323672797	8.6	8.0	14.4	7.0	21.0
285-289	11.58200652254312	8.0	7.8	13.6	7.0	20.2
290-294	11.297304912080154	8.0	7.6	13.0	7.0	19.2
295-299	10.962834280334869	8.0	7.0	13.0	7.0	19.0
300-301	10.608148135695792	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.09378963316476074
1101	2	0.11978870910097328
1101	3	0.11293744489920243
1101	4	0.09375789739854667
1101	5	0.169852010112173
1101	6	0.3380721455465334
1101	7	1.0188409613274345
1101	8	0.3202630489132403
1101	9	0.20420051027511477
1101	10-14	0.23963630598732522
1101	15-19	0.2706589818468501
1101	20-24	0.20433919161727943
1101	25-29	0.1339956007381531
1101	30-34	0.5586963288628866
1101	35-39	0.19845709409626267
1101	40-44	0.5989190526983563
1101	45-49	0.2444086268294825
1101	50-54	0.21527806335223687
1101	55-59	0.3059166556801074
1101	60-64	0.19711522409472337
1101	65-69	0.36273431811958545
1101	70-74	0.22745150413293658
1101	75-79	0.2880029283353096
1101	80-84	0.8096430066104716
1101	85-89	0.4722551119691758
1101	90-94	0.42948469708257875
1101	95-99	1.1267072440031782
1101	100-104	0.4543301059545044
1101	105-109	0.5971779264249548
1101	110-114	0.8112941537450453
1101	115-119	0.7275425745585196
1101	120-124	1.1164354901148599
1101	125-129	0.8210751913967584
1101	130-134	1.0746369340134976
1101	135-139	0.789627109734802
1101	140-144	1.3287987186175165
1101	145-149	1.323307181490069
1101	150-154	1.3899398766520967
1101	155-159	0.8506644872473608
1101	160-164	1.242732318809768
1101	165-169	0.9152536281754209
1101	170-174	0.9811266530485518
1101	175-179	1.244911695963257
1101	180-184	0.9132688452980702
1101	185-189	0.7617376934133162
1101	190-194	0.7391111353319779
1101	195-199	1.4081936265572566
1101	200-204	1.9812706004406415
1101	205-209	1.142751711291229
1101	210-214	1.495626197643972
1101	215-219	1.2617181847296912
1101	220-224	0.9926532088921896
1101	225-229	0.8941959434955109
1101	230-234	0.670769502060935
1101	235-239	0.8455177108213228
1101	240-244	0.6054428064510979
1101	245-249	0.37432264785736
1101	250-254	0.8061608040715242
1101	255-259	0.4485299469580202
1101	260-264	0.6541843111862562
1101	265-269	0.6000587991482469
1101	270-274	0.4742371604107323
1101	275-279	0.09048205262588205
1101	280-284	0.3088033079538661
1101	285-289	0.26181697578875074
1101	290-294	0.09476379972164217
1101	295-299	0.0618811399684418
1101	300-301	-0.19772301187123809
1102	1	-0.29408111960415084
1102	2	-0.04165635512708832
1102	3	-0.11262672952221209
1102	4	-0.06946383018867408
1102	5	-0.14186190015627176
1102	6	-0.0790129867981264
1102	7	0.9600451368215417
1102	8	0.015268613556969513
1102	9	-0.02465216745826382
1102	10-14	-0.10338174223065977
1102	15-19	0.05282899176935274
1102	20-24	-0.030214564182010406
1102	25-29	-0.13464360921653196
1102	30-34	0.28568283963013386
1102	35-39	0.11041604982047204
1102	40-44	0.35353670133468285
1102	45-49	0.03545759569429663
1102	50-54	-0.07457383667190243
1102	55-59	0.14370729098811097
1102	60-64	0.16726593878270535
1102	65-69	0.11351839653413265
1102	70-74	0.1513248313876261
1102	75-79	0.1836387056422879
1102	80-84	0.5733514729142044
1102	85-89	0.09582405991629628
1102	90-94	0.230580529633734
1102	95-99	0.7473668218947935
1102	100-104	0.428147786771909
1102	105-109	0.5978584220134664
1102	110-114	0.49852933033867686
1102	115-119	0.3450484073778455
1102	120-124	0.8983450344845068
1102	125-129	0.5636178983612794
1102	130-134	0.6827492459553639
1102	135-139	0.7249351094129963
1102	140-144	1.5634905848515253
1102	145-149	1.4565622969396692
1102	150-154	1.673017660985284
1102	155-159	1.0363579893525419
1102	160-164	1.130674808552051
1102	165-169	1.2767251747441115
1102	170-174	1.1752921576209445
1102	175-179	0.9868976033354002
1102	180-184	1.3915141052943198
1102	185-189	0.8079084810450574
1102	190-194	1.507570667633729
1102	195-199	1.5974454836732974
1102	200-204	1.6391405486827466
1102	205-209	1.2224374094864068
1102	210-214	1.5870385444486566
1102	215-219	1.702740671253867
1102	220-224	1.2961039584429983
1102	225-229	1.1019358265709478
1102	230-234	0.8101561174166676
1102	235-239	0.5131804263674624
1102	240-244	0.6536611147994016
1102	245-249	0.5436205641329401
1102	250-254	0.8818617480496371
1102	255-259	0.6205666065627291
1102	260-264	0.5235770945808227
1102	265-269	0.2976676784424086
1102	270-274	0.37856182720254594
1102	275-279	0.31216069780274225
1102	280-284	0.3359094920832355
1102	285-289	0.33343159404078015
1102	290-294	-0.051504872004603186
1102	295-299	-0.014480186428082575
1102	300-301	-0.18484555471229136
1103	1	-0.022304144771943868
1103	2	0.009373266328118746
1103	3	0.1112404895213075
1103	4	0.17136447194325655
1103	5	-0.0011653329952849845
1103	6	0.26178258716998926
1103	7	0.9561078893752466
1103	8	0.38510015396802544
1103	9	0.4138295746668348
1103	10-14	0.1720926927973636
1103	15-19	0.23724380726637406
1103	20-24	0.22069828844750106
1103	25-29	0.151665328203606
1103	30-34	0.57042016733444
1103	35-39	0.1793020916697543
1103	40-44	0.4580276961557388
1103	45-49	0.16531933153689948
1103	50-54	0.2727117815203499
1103	55-59	0.39585624853852863
1103	60-64	0.24016521004655544
1103	65-69	0.32631922016550874
1103	70-74	0.21711172992538508
1103	75-79	0.2911327973041722
1103	80-84	0.7255959835459294
1103	85-89	0.4592040404767488
1103	90-94	0.26421784566115747
1103	95-99	0.9711307580896644
1103	100-104	0.5863577042278578
1103	105-109	0.8495603687635978
1103	110-114	0.7165785015267048
1103	115-119	0.7279110198089569
1103	120-124	1.081963190556742
1103	125-129	0.933371812172723
1103	130-134	1.1102247861756425
1103	135-139	1.1653390966572417
1103	140-144	1.6295873319351806
1103	145-149	1.610192573944925
1103	150-154	1.958600000276018
1103	155-159	0.704172060489423
1103	160-164	1.2544718018370205
1103	165-169	1.648246080475488
1103	170-174	1.7852405326429412
1103	175-179	2.110713016491463
1103	180-184	1.6489183178530524
1103	185-189	1.2991246694803387
1103	190-194	1.3232344655151174
1103	195-199	1.496515444646196
1103	200-204	1.7047409460256802
1103	205-209	1.2090948442891047
1103	210-214	1.7712494256585813
1103	215-219	2.0383372366057593
1103	220-224	1.428617168944296
1103	225-229	0.9158017134630718
1103	230-234	1.5037728097669927
1103	235-239	1.2518860028189707
1103	240-244	0.8638956556758934
1103	245-249	1.0115030519338877
1103	250-254	0.8592855646140372
1103	255-259	0.7721519489503059
1103	260-264	0.806019832628877
1103	265-269	0.8135645205857553
1103	270-274	0.5631272909310265
1103	275-279	0.3854125780275304
1103	280-284	0.45322203289065044
1103	285-289	0.36604068654964905
1103	290-294	0.08016168227892173
1103	295-299	0.29592720520304816
1103	300-301	0.07441084167017742
2116	1	0.16778185381539856
2116	2	0.13370566202075196
2116	3	0.0535693907468513
2116	4	0.043773051975229293
2116	5	0.0777374415124541
2116	6	0.24781401259037494
2116	7	0.850031605902096
2116	8	0.17408452799992347
2116	9	0.2833958022532954
2116	10-14	0.15549304472241232
2116	15-19	0.22387932939181354
2116	20-24	0.10393506957242238
2116	25-29	0.11140770419339674
2116	30-34	0.46363166933571165
2116	35-39	0.042160367484825656
2116	40-44	0.44783550046558673
2116	45-49	0.1892257616041917
2116	50-54	0.18491081744397064
2116	55-59	0.3736626649749155
2116	60-64	0.34408936028385284
2116	65-69	0.4615547869011536
2116	70-74	0.4680228316738564
2116	75-79	0.42062820616921215
2116	80-84	0.8568141522964723
2116	85-89	0.4787842087563945
2116	90-94	0.6072605103781967
2116	95-99	1.3546169227261515
2116	100-104	0.5764886228266022
2116	105-109	0.5408483648973714
2116	110-114	0.8606450327104938
2116	115-119	0.786392847340899
2116	120-124	1.2019931785367675
2116	125-129	1.0052336072383454
2116	130-134	0.9357452387215162
2116	135-139	1.3236743920140555
2116	140-144	1.9305414949359623
2116	145-149	1.904636743017658
2116	150-154	2.2912456960095433
2116	155-159	1.9667586476838181
2116	160-164	1.3156132038370494
2116	165-169	1.6142357457749377
2116	170-174	1.3779820783870065
2116	175-179	1.8465458644821489
2116	180-184	1.8301182088058532
2116	185-189	1.4751974832698558
2116	190-194	1.7703775527900518
2116	195-199	0.7569938892365897
2116	200-204	0.7507316026633113
2116	205-209	0.8257381732027262
2116	210-214	1.8795825524631269
2116	215-219	2.179032288589056
2116	220-224	1.7503466823211689
2116	225-229	0.701053389444187
2116	230-234	1.3423925572235902
2116	235-239	0.7458410084572051
2116	240-244	0.6217870174027986
2116	245-249	0.7898813465843784
2116	250-254	1.0255106727318601
2116	255-259	1.292983371892353
2116	260-264	0.4109003144192336
2116	265-269	0.3579619108879921
2116	270-274	0.5939860995903654
2116	275-279	0.6832533195484913
2116	280-284	0.13051729057661987
2116	285-289	0.12453924291341956
2116	290-294	0.17537351885682284
2116	295-299	0.33356986496339225
2116	300-301	0.12999470888042808
1117	1	0.04319439506952705
1117	2	-0.0736235614676346
1117	3	0.07160289221020122
1117	4	0.08546307003364007
1117	5	0.2339131062487354
1117	6	0.2177778957711496
1117	7	0.980403982762418
1117	8	0.27580630395110006
1117	9	0.18050489995551544
1117	10-14	0.0148949377419072
1117	15-19	0.042945273722089894
1117	20-24	0.14491613937751424
1117	25-29	0.002247108888582261
1117	30-34	0.3658276342152149
1117	35-39	0.16958050572505812
1117	40-44	0.3715904042578515
1117	45-49	0.06781191267211995
1117	50-54	0.06275688506959654
1117	55-59	0.056195823951185275
1117	60-64	0.27137655126710314
1117	65-69	0.22075928282244206
1117	70-74	0.25957927977150064
1117	75-79	0.28692187327177265
1117	80-84	0.48921623212709875
1117	85-89	0.2555586031769721
1117	90-94	0.2046351912425095
1117	95-99	0.811993471701804
1117	100-104	0.2809624381326543
1117	105-109	0.2782336317259393
1117	110-114	0.497427833632095
1117	115-119	0.387168424898654
1117	120-124	0.641972006608249
1117	125-129	0.6236223612080707
1117	130-134	0.6216039472338792
1117	135-139	0.8138730345198155
1117	140-144	1.2287929427222615
1117	145-149	1.7229747466293297
1117	150-154	1.757298688101212
1117	155-159	1.2641049955261465
1117	160-164	1.642646643030023
1117	165-169	1.1872427823276475
1117	170-174	0.9476806897575756
1117	175-179	1.361249457482959
1117	180-184	1.4040213160977117
1117	185-189	1.1420223166965933
1117	190-194	-1.001177338548139
1117	195-199	0.9910414638053666
1117	200-204	1.303533146968686
1117	205-209	1.3772581931292471
1117	210-214	1.7847039797001898
1117	215-219	1.3320162851019148
1117	220-224	0.7921070659066949
1117	225-229	1.1318557432644774
1117	230-234	0.8976765101471855
1117	235-239	0.4295152721099882
1117	240-244	0.18066646195103075
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2108	265-269	-0.2970276670171703
2108	270-274	-0.4579996816945311
2108	275-279	-0.4373464471951003
2108	280-284	-0.7583422344736306
2108	285-289	-1.237811559836027
2108	290-294	-1.0366764079403694
2108	295-299	-0.6336210082592704
2108	300-301	2.454957797294533
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	28.0
11	190.0
12	412.0
13	499.0
14	597.0
15	995.0
16	1319.0
17	2006.0
18	2784.0
19	2959.0
20	3797.0
21	5129.0
22	7030.0
23	9687.0
24	13813.0
25	19362.0
26	27841.0
27	38016.0
28	47768.0
29	48823.0
30	38367.0
31	19722.0
32	5614.0
33	646.0
34	21.0
35	3.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.214168039538713	22.215647379215277	14.239989241165988	33.330195340080024
2	45.69982853108294	23.438792320882225	17.00164744645799	13.859731701576841
3	44.94637393672461	22.482264734559394	18.490401102780485	14.080960225935515
4	45.25400934673704	21.483374239316813	18.600006724271257	14.662609689674882
5	28.985643680866087	21.50421948021383	35.08623877887234	14.423898060047744
6	27.860673099552834	21.232222707864036	35.44228894193592	15.46481525064721
7	16.55818175705208	48.24664626971052	20.020845240897017	15.174326732340383
8	27.212117136805297	20.17247755774468	36.60121709309754	16.014188212352487
9	15.343778368019365	18.961436304340516	37.745688061056384	27.949097266583735
10-14	26.439296641226505	34.36734693877551	23.43253874861312	15.760817671384864
15-19	21.477591366035707	35.101099418350536	21.89503412567663	21.526275089937126
20-24	25.05416400497596	31.03647917156978	26.64593349695727	17.26342332649699
25-29	19.076892041824966	25.560703358773495	38.11848165955015	17.243922939851394
30-34	19.228927814948054	28.472178327673735	31.382980869448275	20.915912987929932
35-39	20.590122045523316	29.728608412063345	27.987492855461788	21.69377668695155
40-44	19.85845409003799	29.063107285747908	29.592374676394446	21.486063947819655
45-49	20.921090676797903	30.56288874693205	26.208250680832464	22.30776989543758
50-54	21.922132938842754	29.52304743973372	27.843055508859226	20.711764112564303
55-59	21.492586490939043	26.76098577816629	30.142756278788283	21.603671452106376
60-64	21.93887637427294	25.72833944121306	29.59640923914871	22.736374945365295
65-69	20.048011296775712	26.493427024846184	29.52224052718287	23.936321151195237
70-74	20.817133443163097	25.895101368389202	29.475103385670575	23.812661802777125
75-79	20.13253538647749	25.681202299700768	30.870187943381637	23.316074370440106
80-84	22.95565343105941	25.600107588340116	27.503614295800695	23.940624684799786
85-89	20.555424805836665	28.13307332817806	25.999058602024007	25.31244326396127
90-94	20.022526308711296	30.110412534041625	28.814578220085394	21.052482937161685
95-99	22.00712772753253	30.109605621490772	26.434925864909392	21.44834078606731
100-104	22.570957872440577	28.438220757825373	26.527855293682546	22.462966076051508
105-109	23.633796187338195	27.55310493225297	23.795514911071514	25.01758396933732
110-114	21.380963588071143	27.962276838247657	29.212722321218436	21.444037252462763
115-119	23.417947079985208	27.829875937195308	27.0048750966614	21.747301886158088
120-124	18.692330968631275	28.738795683017855	28.117607504286724	24.45126584406415
125-129	20.55623171838752	24.851292741149177	30.958948323975388	23.633527216487913
130-134	25.12698786269038	28.708738190498607	25.918569075076487	20.245704871734528
135-139	23.950711091685438	27.529569982853108	24.83071647110244	23.68900245435901
140-144	21.30592072084188	28.998756009817434	26.503580674444404	23.19174259489628
145-149	20.99169552499748	26.89748848468547	29.17533537302895	22.9354806172881
150-154	23.380627374508286	25.59445920048415	25.70957872440574	25.31533470060182
155-159	22.100191641730827	26.937968597653228	29.739770702350132	21.22206905826581
160-164	24.836095888108126	28.973271021753018	26.19688666240796	19.993746427730894
165-169	20.27159331607437	25.474229230407154	28.709545103049454	25.544632350469016
170-174	22.560064553004068	28.839995965437247	26.24462898833339	22.355310493225296
175-179	20.33392731062771	29.26987862690381	28.60807584977978	21.7881182126887
180-184	19.99939481558686	29.472010220892308	26.834078606730994	23.694516356789833
185-189	22.31294758430555	27.46535319234778	27.874726826480178	22.34697239686649
190-194	20.385905927445116	27.921729482567326	26.56335944592005	25.129005144067513
195-199	21.18064754732206	29.7526140604512	27.425209292942874	21.641529099283865
200-204	22.598527384594693	27.704132064687492	27.560098174360352	22.137242376357463
205-209	21.583700366472783	27.42184715731433	28.172141344181824	22.822311132031068
210-214	23.112747949263994	27.527638454519355	27.509079453370173	21.85053414284648
215-219	23.48700534579565	26.981003933698684	28.112833271694182	21.419157448811486
220-224	19.7191944323034	27.239552163534274	28.728507547994486	24.31274585616784
225-229	21.87203711797734	28.724338499815083	26.6386712839996	22.764953098207982
230-234	22.186396799246882	26.524022459066	28.586827152607334	22.702753589079784
235-239	22.157885889116766	27.518138721716035	25.938540160710083	24.385435228457116
240-244	23.131762095282923	31.518273207141178	23.518273207141178	21.831691490434725
245-249	21.28964798439969	29.91937598762734	24.625357226910534	24.165618801062436
250-254	22.21927848569411	27.928520996537	25.307400060518443	24.544800457250446
255-259	22.049356151027133	28.289076421342834	27.36045456073698	22.30111286689305
260-264	21.559560232659784	29.464815250647213	26.72440574252765	22.25121877416535
265-269	20.79944860975692	29.194163332548833	27.234172746528596	22.772215311165652
270-274	20.001546582389135	31.21998453417611	25.089735399926035	23.688733483508724
275-279	21.084019769357496	30.238039202501426	24.474868036176577	24.203072991964497
280-284	18.712234811552296	30.989476515482632	27.250512725683357	23.047775947281714
285-289	20.91107151262482	29.757724506606596	27.703728608412064	21.62747537235652
290-294	20.109269407927915	32.02077799818445	25.586524560400765	22.28342803348687
295-299	19.41478667249437	33.333490232996	23.31318293379955	23.938540160710083
300-301	14.797095114816933	35.46565578455435	30.755135662172613	18.982113438456107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.25
5	0.25
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.25
13	0.5
14	0.25
15	0.25
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.25
22	0.25
23	0.5
24	0.0
25	0.25
26	0.75
27	0.5
28	1.0
29	0.5
30	0.75
31	3.0
32	5.25
33	18.5
34	61.5
35	183.5
36	495.25
37	1226.5
38	2252.5
39	3534.25
40	5090.75
41	7005.75
42	9275.25
43	11779.25
44	12891.5
45	11395.0
46	8738.75
47	6796.0
48	5479.25
49	4375.75
50	3525.0
51	2496.75
52	1315.75
53	544.5
54	227.0
55	102.25
56	64.75
57	49.75
58	30.75
59	22.0
60	21.0
61	17.75
62	11.0
63	12.0
64	8.5
65	6.0
66	4.75
67	3.5
68	5.75
69	3.5
70	3.5
71	2.5
72	2.5
73	1.0
74	0.75
75	0.25
76	1.5
77	1.75
78	1.5
79	0.0
80	1.0
81	0.25
82	0.25
83	0.5
84	0.75
85	0.25
86	1.0
87	1.25
88	1.0
89	0.5
90	0.75
91	0.0
92	0.0
93	0.0
94	0.0
95	0.25
96	0.75
97	0.0
98	0.5
99	0.25
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	6.724271257102511E-5
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	297430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.105403714166298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.01127968962595	6.9501895511722
2	7.78942183635825	2.3532472307626793
3	5.706732046611441	2.586074743579089
4	5.588443322784317	3.376627700975763
5	4.789342350736648	3.617247486661565
6	4.320624992700837	3.9158870887357775
7	3.8375413991299836	4.05773284725795
8	3.5920674321943866	4.340770298544391
9	2.813478906712449	3.8248861254464486
>10	15.293960915395447	37.84625705967045
>50	0.1743858396917165	1.6735176147070812
>100	0.0321964948833424	1.0595387123707458
>500	0.013779483593233851	1.6585660535376392
>1k	0.025262386587592646	9.951049413453969
>5k	0.009186322395488236	8.89332761942848
>10k+	0.002296580598872059	3.8950804536957007
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11228	3.77500588373735	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8116	2.728709276132199	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7201	2.4210738661197593	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5190	1.7449483912181016	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5129	1.724439363883939	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3905	1.3129139629492654	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3790	1.274249403220926	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3579	1.2033083414584944	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3235	1.0876508758363312	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2935	0.9867868069797935	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2732	0.9185354537202031	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2126	0.714790034629997	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1851	0.6223313048448375	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1800	0.6051844131392261	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1705	0.5732441246679891	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1027	0.34529132905221394	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	966	0.3247823017180513	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	930	0.3126786134552668	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	916	0.307971623575295	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	752	0.2528325992670544	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	641	0.21551289379013547	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	576	0.19365901220455234	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	470	0.15802037454190904	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	430	0.14457183202770402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.724271257102512E-4	0.0	0.0
2	6.724271257102512E-4	0.0	0.0
3	6.724271257102512E-4	0.0	0.0
4	6.724271257102512E-4	0.0	0.0
5	6.724271257102512E-4	0.0	0.0
6	6.724271257102512E-4	0.0	0.0
7	6.724271257102512E-4	0.0	0.0
8	6.724271257102512E-4	0.0	0.0
9	6.724271257102512E-4	0.0	0.0
10-14	0.001143126113707427	0.0	0.0
15-19	0.001748310526846653	0.0	0.0
20-24	0.0020845240897017785	0.0	0.0
25-29	0.002958679353125105	0.0	0.0
30-34	0.006186329556534311	0.0	0.0
35-39	0.007665669233096863	0.0	0.0
40-44	0.009817436035369668	0.0	0.0
45-49	0.012910600813636822	0.0	0.0
50-54	0.017752076118750633	0.0	0.0
55-59	0.02709881316612312	0.0	0.0
60-64	0.038126618027771245	0.0	0.0
65-69	0.05379417005682009	0.0	0.0
70-74	0.07941364354638067	0.0	0.0
75-79	0.11901960125071445	0.0	0.0
80-84	0.17631039236122784	0.0	0.0
85-89	0.2444945029082473	0.0	0.0
90-94	0.3296237770231651	0.0	0.0
95-99	0.42974817604142157	0.0	0.0
100-104	0.5522643983458292	0.0	0.0
105-109	0.7008035504152237	0.0	0.0
110-114	0.8858554954106849	0.0	0.0
115-119	1.10594089365565	0.0	0.0
120-124	1.3467370473724911	0.0	0.0
125-129	1.6080422284234945	0.0	0.0
130-134	1.9139293279090879	0.0	0.0
135-139	2.201997108563359	0.0	0.0
140-144	2.544128030124735	0.0	0.0
145-149	2.9552499747839827	0.0	0.0
150-154	3.402279527956158	0.0	0.0
155-159	3.8916719900480787	0.0	0.0
160-164	4.409978818545541	0.0	0.0
165-169	4.971052012238173	0.0	0.0
170-174	5.5405977877147565	0.0	0.0
175-179	6.140402783848301	0.0	0.0
180-184	6.7451837407121005	0.0	0.0
185-189	7.4200988467874796	0.0	0.0
190-194	8.112295329993612	0.0	0.0
195-199	8.783781057727868	0.0	0.0
200-204	9.428167972296002	0.0	0.0
205-209	10.088693137881183	0.0	0.0
210-214	10.736307702652725	0.0	0.0
215-219	11.383586053861412	0.0	0.0
220-224	12.03926974414148	0.0	0.0
225-229	12.687825706889019	0.0	0.0
230-234	13.289110042699122	0.0	0.0
235-239	13.820327472010224	0.0	0.0
240-244	14.264532831254414	0.0	0.0
245-249	14.635779847359043	0.0	0.0
250-254	14.979390108596983	0.0	0.0
255-259	15.286689305046565	0.0	0.0
260-264	15.545775476582724	0.0	0.0
265-269	15.752076118750631	0.0	0.0
270-274	15.905389503412568	0.0	0.0
275-279	16.00981743603537	0.0	0.0
280-284	16.09595535083885	0.0	0.0
285-289	16.15149783142252	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4800	0.0	290.39062	1
GGGTTAT	4820	0.0	289.79773	2
GTTATTA	4850	0.0	286.48453	4
GGTTATT	4875	0.0	285.6205	3
GGGGGGA	3675	0.0	280.9524	1
TTATTAG	4960	0.0	279.83368	5
TTAGTAG	5085	0.0	274.98526	8
GGGGAGC	3785	0.0	272.0079	3
TAGTAGA	5125	0.0	271.6878	9
GGAGCGA	2810	0.0	268.22952	5
GGGAGCG	2820	0.0	267.27835	4
TATTAGT	5220	0.0	266.17816	6
ATTAGTA	5305	0.0	261.3572	7
CGAAAGC	3045	0.0	252.37276	9
GAGCGAA	3095	0.0	243.52989	6
GGGGGAG	4280	0.0	241.58296	2
GCGAAAG	3200	0.0	240.60938	8
AGCGAAA	3140	0.0	239.57007	7
GGGAGCA	1325	0.0	202.60376	4
GCAAAAG	1555	0.0	160.30547	8
>>END_MODULE