##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30291_flu.3520000003ba0c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233962
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1277557894017	34.0	34.0	34.0	34.0	34.0
2	33.46468657303323	34.0	34.0	34.0	34.0	34.0
3	33.435310007608074	34.0	34.0	34.0	34.0	34.0
4	33.47408126105949	34.0	34.0	34.0	34.0	34.0
5	33.459130115146905	34.0	34.0	34.0	34.0	34.0
6	36.96048076183312	38.0	38.0	38.0	36.0	38.0
7	36.15608945042357	38.0	38.0	38.0	34.0	38.0
8	36.88690471102145	38.0	38.0	38.0	35.0	38.0
9	36.94666655268804	38.0	38.0	38.0	36.0	38.0
10-14	36.9821500927501	38.0	38.0	38.0	36.2	38.0
15-19	36.891295167591316	38.0	38.0	38.0	36.0	38.0
20-24	36.98812542207709	38.0	38.0	38.0	36.0	38.0
25-29	36.911995965156734	38.0	38.0	38.0	36.0	38.0
30-34	36.56874877116797	38.0	37.8	38.0	34.6	38.0
35-39	36.83792154281464	38.0	38.0	38.0	35.8	38.0
40-44	36.369978885460036	38.0	37.2	38.0	33.6	38.0
45-49	36.67879741154546	38.0	38.0	38.0	34.8	38.0
50-54	36.628869645497986	38.0	37.8	38.0	34.6	38.0
55-59	36.2821603508262	38.0	37.0	38.0	33.4	38.0
60-64	36.45041160530342	38.0	37.0	38.0	34.0	38.0
65-69	36.33779844590147	38.0	37.0	38.0	33.8	38.0
70-74	36.18952137526607	38.0	37.0	38.0	33.4	38.0
75-79	36.22597259384003	38.0	37.0	38.0	33.4	38.0
80-84	35.59053008608235	38.0	36.8	38.0	29.8	38.0
85-89	35.7973995777092	38.0	37.0	38.0	31.4	38.0
90-94	35.76842393209154	38.0	37.0	38.0	31.2	38.0
95-99	34.86052264897718	38.0	35.8	38.0	26.2	38.0
100-104	35.18785956693822	38.0	36.0	38.0	27.4	38.0
105-109	34.983577675007055	38.0	35.8	38.0	26.8	38.0
110-114	34.49565314025355	38.0	35.0	38.0	24.0	38.0
115-119	34.552738478898284	38.0	35.0	38.0	24.4	38.0
120-124	34.098376659457514	37.6	34.6	38.0	23.4	38.0
125-129	33.6201853292415	37.0	34.0	38.0	23.0	38.0
130-134	33.37762029731324	37.0	33.2	38.0	22.6	38.0
135-139	32.72754464400201	37.0	30.6	38.0	21.0	38.0
140-144	31.453670254143834	35.8	27.2	38.0	16.8	38.0
145-149	31.284748805361552	35.0	28.0	37.6	17.2	38.0
150-154	30.236314444225986	35.0	25.0	37.0	11.4	38.0
155-159	30.05376172198904	35.0	24.8	37.0	11.2	37.4
160-164	29.31164890024876	34.2	23.4	37.0	10.2	37.4
165-169	28.249711491609748	32.2	22.0	36.6	9.8	37.0
170-174	27.54369256545935	31.2	20.6	36.0	9.2	37.0
175-179	26.68979834332071	29.2	20.0	35.4	9.2	37.0
180-184	25.79217052341833	27.6	19.0	35.0	9.0	36.8
185-189	25.031894068267498	26.4	17.6	35.0	8.4	36.4
190-194	23.993610928270403	25.2	15.6	34.2	8.2	36.0
195-199	23.13543395936092	24.2	15.0	33.2	8.0	36.0
200-204	22.280810558979663	23.0	12.8	31.4	8.0	36.0
205-209	21.360627794257187	21.8	9.6	30.4	8.0	35.2
210-214	20.101824227866064	20.2	9.0	28.2	8.0	34.4
215-219	19.625717851616926	19.6	9.0	27.6	8.0	34.0
220-224	18.907137911284742	18.2	9.0	26.2	8.0	33.2
225-229	18.175617407955137	16.8	9.0	25.4	8.0	32.2
230-234	17.26854019028731	15.6	9.0	24.6	7.6	31.0
235-239	16.335429685162545	14.6	8.4	22.8	7.0	29.4
240-244	15.471362870893566	13.2	8.0	21.2	7.0	28.2
245-249	14.868627383934143	12.8	8.0	20.2	7.0	26.8
250-254	14.151023670510593	11.8	8.0	19.2	7.0	25.6
255-259	13.563652217026695	11.2	8.0	18.0	7.0	24.4
260-264	12.986352484591515	10.2	7.8	16.4	7.0	23.2
265-269	12.59853651447671	9.0	7.6	15.8	7.0	22.4
270-274	12.152032381326881	9.0	7.4	14.6	7.0	21.4
275-279	11.653656576709038	8.0	7.0	14.0	7.0	20.6
280-284	11.54286508065412	8.0	7.0	13.6	7.0	20.2
285-289	11.234291038715687	8.0	7.0	13.0	7.0	19.0
290-294	10.969622417315632	8.0	7.0	13.0	7.0	19.0
295-299	10.69904001504518	8.0	7.0	13.0	7.0	18.4
300-301	10.393657944452517	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.010364888231812586
1101	2	0.0937702606561146
1101	3	0.14987995760066752
1101	4	0.21328353304935632
1101	5	0.01864912647229744
1101	6	0.4619090051823491
1101	7	1.067408475989211
1101	8	0.39018689250595173
1101	9	0.07493115380576398
1101	10-14	0.18999718257968112
1101	15-19	0.21417784723156075
1101	20-24	0.23079124664656803
1101	25-29	0.30615318744189324
1101	30-34	0.5474327094300833
1101	35-39	0.2528361904429346
1101	40-44	0.6334653852255769
1101	45-49	0.35367596518160127
1101	50-54	0.280445268297413
1101	55-59	0.4920009679002604
1101	60-64	0.35005870900589287
1101	65-69	0.5006146653272694
1101	70-74	0.34428331544931723
1101	75-79	0.576188412102077
1101	80-84	0.9068290776393013
1101	85-89	0.570616630364654
1101	90-94	0.4638943952893513
1101	95-99	1.0722854382913596
1101	100-104	0.5383511714971476
1101	105-109	0.6383088006067865
1101	110-114	0.7310114756796438
1101	115-119	0.6658933613829703
1101	120-124	1.063102781015374
1101	125-129	0.8893767330927034
1101	130-134	1.153634853340634
1101	135-139	0.7948979293874601
1101	140-144	1.6191606232990523
1101	145-149	1.6557148525812124
1101	150-154	1.495544342764454
1101	155-159	1.0718954744062223
1101	160-164	1.4719445193518261
1101	165-169	1.0939276412643615
1101	170-174	1.0003547948722407
1101	175-179	0.9815683684538037
1101	180-184	0.7358437577307946
1101	185-189	1.0605268512638126
1101	190-194	1.2257192697651504
1101	195-199	1.6292485742903722
1101	200-204	1.557465291891333
1101	205-209	1.1765485565827305
1101	210-214	1.7173190064638355
1101	215-219	1.54094233240912
1101	220-224	1.4931096832109567
1101	225-229	0.7659023563858192
1101	230-234	0.7430290641973016
1101	235-239	0.8790787216159366
1101	240-244	0.5652777828565938
1101	245-249	0.8566389473915752
1101	250-254	0.5359745099863211
1101	255-259	0.31693813047474606
1101	260-264	0.7159771612720274
1101	265-269	0.507187789093603
1101	270-274	0.03698596609278759
1101	275-279	0.454821879872231
1101	280-284	0.34036900859709895
1101	285-289	0.30708439962342915
1101	290-294	0.28443899393827543
1101	295-299	3.618669870988356E-4
1101	300-301	-0.26892397802731516
1102	1	-0.012990672634920486
1102	2	-0.047443624811698726
1102	3	0.1228383769051149
1102	4	0.03984847389590129
1102	5	0.02412195426882846
1102	6	0.00833607235444589
1102	7	0.8476076623959159
1102	8	0.22557286948435262
1102	9	0.17486291006899535
1102	10-14	0.04131647263155713
1102	15-19	0.24528419549854874
1102	20-24	0.12984329849047782
1102	25-29	0.09333136224182681
1102	30-34	0.5453427449914727
1102	35-39	0.2566722348653414
1102	40-44	0.6432903507038432
1102	45-49	0.05482811139381738
1102	50-54	0.21606529996677182
1102	55-59	0.29868672148705144
1102	60-64	0.26497316441553664
1102	65-69	0.08796185000654333
1102	70-74	0.20482937197431994
1102	75-79	0.3056894330923612
1102	80-84	0.6806757318301564
1102	85-89	0.22910844378201034
1102	90-94	0.25952572982762234
1102	95-99	0.8128515414140409
1102	100-104	0.6527388705635886
1102	105-109	0.7131066018788914
1102	110-114	0.7214856097039473
1102	115-119	0.7370854408442753
1102	120-124	0.9565636448318031
1102	125-129	0.7249658418188432
1102	130-134	0.8236502614968302
1102	135-139	1.0570290533298135
1102	140-144	1.8596622147643203
1102	145-149	1.8957104807168292
1102	150-154	1.7670936355354137
1102	155-159	1.1429441353894667
1102	160-164	1.3898147816103688
1102	165-169	1.2165925591852726
1102	170-174	1.2698295846761987
1102	175-179	1.0440391116174403
1102	180-184	1.4007664770304444
1102	185-189	1.4008402392987698
1102	190-194	1.6433181565392019
1102	195-199	1.1554237794214473
1102	200-204	1.6116716119011407
1102	205-209	1.051519099813536
1102	210-214	1.398370706425979
1102	215-219	1.2270046154820058
1102	220-224	1.1588646562066955
1102	225-229	1.0007071952932804
1102	230-234	0.7650872526459835
1102	235-239	0.7114625073306016
1102	240-244	0.5591699684155813
1102	245-249	0.42464169846721056
1102	250-254	0.7662000875256005
1102	255-259	0.4524312714460468
1102	260-264	0.6233053656620164
1102	265-269	0.5256349241895464
1102	270-274	0.5419139103035331
1102	275-279	0.08692842540626344
1102	280-284	0.42041091451670987
1102	285-289	0.34990841075569
1102	290-294	0.10424140699415396
1102	295-299	-0.14865686271972045
1102	300-301	-0.0699281739508173
1103	1	-0.24948692602281142
1103	2	0.014508333523849615
1103	3	0.18665832695028683
1103	4	0.11746977378430046
1103	5	0.1299916465683424
1103	6	-0.0620444346339184
1103	7	0.926329031370777
1103	8	0.1812193593605258
1103	9	-0.03655229958185657
1103	10-14	-0.05444586993102973
1103	15-19	0.08767299286262897
1103	20-24	-0.009890452904535607
1103	25-29	-0.018104041913993285
1103	30-34	0.41349388491813244
1103	35-39	0.1076665485178907
1103	40-44	0.34857865383399655
1103	45-49	-0.03154378635821331
1103	50-54	-0.012464221210883863
1103	55-59	0.13661731616208073
1103	60-64	0.12517510946522492
1103	65-69	0.1869138614855288
1103	70-74	0.09667291770424669
1103	75-79	0.161025033878893
1103	80-84	0.707466931277466
1103	85-89	0.27876779436924437
1103	90-94	0.22954007855483383
1103	95-99	0.9147418533659959
1103	100-104	0.4313619241853175
1103	105-109	0.4704488987381197
1103	110-114	0.5251998043152
1103	115-119	0.40550814371933797
1103	120-124	0.8484603339958525
1103	125-129	0.9276750940907235
1103	130-134	0.8019634888975844
1103	135-139	1.0041901310786656
1103	140-144	1.361951102472247
1103	145-149	1.4388300003001788
1103	150-154	1.8560339554588872
1103	155-159	0.704937545603638
1103	160-164	0.8720186398236898
1103	165-169	1.439453270244421
1103	170-174	1.8432371416725353
1103	175-179	1.5791578871927108
1103	180-184	1.0920583939141153
1103	185-189	1.4997731191414552
1103	190-194	1.222850494318866
1103	195-199	1.4833575627025226
1103	200-204	1.528402759267884
1103	205-209	1.199127113843403
1103	210-214	1.8100760792271693
1103	215-219	1.8200215682374932
1103	220-224	1.1062436220354641
1103	225-229	0.8453960404864596
1103	230-234	1.3975483250804785
1103	235-239	0.9374491151852027
1103	240-244	0.9520248336969743
1103	245-249	1.3168800999127122
1103	250-254	0.9144064966237586
1103	255-259	1.0806201432109397
1103	260-264	0.6432357477633097
1103	265-269	0.670824911966557
1103	270-274	0.5332935138535149
1103	275-279	0.5212181634091344
1103	280-284	0.5709891150800299
1103	285-289	0.37139695834563646
1103	290-294	0.16560613038258154
1103	295-299	0.2712575763513332
1103	300-301	-0.004786280981694091
2116	1	0.04333205897821557
2116	2	-0.018828424232467
2116	3	-0.08258350580089058
2116	4	-0.09089266714440924
2116	5	0.07941634350690663
2116	6	0.18124712073900184
2116	7	0.9681309627453416
2116	8	0.3411433371702657
2116	9	0.2841688838987295
2116	10-14	0.08717319725615624
2116	15-19	0.2712398718435196
2116	20-24	0.12583381255674198
2116	25-29	0.15870508074883816
2116	30-34	0.43945443556535935
2116	35-39	0.03432362680020162
2116	40-44	0.4599799277193455
2116	45-49	0.28682687318564604
2116	50-54	0.21831243792205868
2116	55-59	0.35969313288696014
2116	60-64	0.36321291509497655
2116	65-69	0.45336547438875385
2116	70-74	0.41849950492429855
2116	75-79	0.5545220546308869
2116	80-84	0.8644363169359792
2116	85-89	0.4745475354828699
2116	90-94	0.5554574814724447
2116	95-99	1.3474683789464308
2116	100-104	0.596843740499267
2116	105-109	0.760656418770985
2116	110-114	0.9379920788169471
2116	115-119	0.8359663315146904
2116	120-124	1.1341352877661492
2116	125-129	0.9497667316497953
2116	130-134	0.9078048696249326
2116	135-139	1.7598753581179025
2116	140-144	2.0787951542495193
2116	145-149	2.108034849283129
2116	150-154	2.69477650733889
2116	155-159	2.2276763991068194
2116	160-164	1.7649855598976565
2116	165-169	1.869611478586318
2116	170-174	1.9145829399055323
2116	175-179	2.0973765023560595
2116	180-184	2.2455019936675527
2116	185-189	1.7469335876025234
2116	190-194	2.264424359122639
2116	195-199	1.233608230465002
2116	200-204	1.1302457837182587
2116	205-209	1.6246274220414705
2116	210-214	1.765005193671385
2116	215-219	1.9045500251924992
2116	220-224	1.5063504640325895
2116	225-229	0.779645064525905
2116	230-234	0.972025415690716
2116	235-239	1.0012892631202739
2116	240-244	0.6817043077055978
2116	245-249	0.9141987767156721
2116	250-254	1.2807820873367213
2116	255-259	1.554540422643857
2116	260-264	0.6164574449073399
2116	265-269	0.8076258160142231
2116	270-274	0.8168480649116638
2116	275-279	0.7395806043378368
2116	280-284	0.19470392913386014
2116	285-289	0.016907539812658356
2116	290-294	0.25039141906143847
2116	295-299	0.19331839410354057
2116	300-301	-0.1712697410186781
1117	1	0.22773399592691845
1117	2	0.1282510830913708
1117	3	0.12372966102986283
1117	4	0.025213744477284195
1117	5	0.07426253290191198
1117	6	0.07577483879642699
1117	7	0.9731591931698986
1117	8	0.18280682247235802
1117	9	0.20748962572596952
1117	10-14	0.19183071498808602
1117	15-19	0.30429759854087024
1117	20-24	0.19328142050545694
1117	25-29	0.27190104610880184
1117	30-34	0.5273434829643406
1117	35-39	0.3253260580324948
1117	40-44	0.5554871711841542
1117	45-49	0.20306087427065478
1117	50-54	0.20027008223879506
1117	55-59	0.3066651435763461
1117	60-64	0.28566966528083526
1117	65-69	0.5035232030953836
1117	70-74	0.49294644980967206
1117	75-79	0.6206515730057802
1117	80-84	0.9304312133574513
1117	85-89	0.576972739927399
1117	90-94	0.47493948834419086
1117	95-99	1.182670010484138
1117	100-104	0.4650598991522443
1117	105-109	0.560098842769861
1117	110-114	0.7006867280966205
1117	115-119	0.5685579045822635
1117	120-124	1.1870356876134451
1117	125-129	1.0164427749699243
1117	130-134	1.0891396362467205
1117	135-139	1.067594018328073
1117	140-144	1.4592880313416856
1117	145-149	1.8217261028747203
1117	150-154	2.365305350594735
1117	155-159	1.9941173563291912
1117	160-164	2.0403477331380273
1117	165-169	1.466809533600486
1117	170-174	1.6370999685780028
1117	175-179	1.5381046664742861
1117	180-184	1.4242106275561213
1117	185-189	1.1994250983823278
1117	190-194	-0.6172288788367233
1117	195-199	1.091047600353889
1117	200-204	1.2802568347741392
1117	205-209	2.060128436729329
1117	210-214	2.1978790709845732
1117	215-219	1.6649691616027127
1117	220-224	1.5554824538254337
1117	225-229	1.1300800742709285
1117	230-234	1.0534636603811833
1117	235-239	0.9464559331357449
1117	240-244	0.9335196947939508
1117	245-249	1.0771375538661054
1117	250-254	0.6978398944053801
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2108	260-264	-0.7142468785202709
2108	265-269	-0.617072609037491
2108	270-274	-0.49191860107232266
2108	275-279	-0.21281253880017736
2108	280-284	-0.6889413900542927
2108	285-289	-1.2167941006731393
2108	290-294	-0.7328483867836884
2108	295-299	-0.5126434712578369
2108	300-301	2.627427700970385
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	31.0
12	163.0
13	299.0
14	494.0
15	849.0
16	1225.0
17	1735.0
18	2106.0
19	2768.0
20	3566.0
21	4852.0
22	6659.0
23	9255.0
24	13163.0
25	18200.0
26	25247.0
27	33153.0
28	38129.0
29	35441.0
30	24020.0
31	10106.0
32	2242.0
33	249.0
34	5.0
35	2.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.11583932433472	21.437669365110573	13.82233012198562	35.62416118856908
2	47.129448371957835	23.110163188893925	16.258623195219737	13.501765243928501
3	46.11774561680957	22.525025431480326	17.83622981509818	13.520999136611927
4	46.57978646104923	21.574016293244203	17.90119763038442	13.944999615322146
5	27.766474897632946	21.893298911789095	36.67561398859644	13.66461220198152
6	26.686812388336566	21.071797984288047	37.15603388584471	15.085355741530677
7	16.303074858310325	49.847838537882225	19.099255434643233	14.749831169164224
8	26.484215385404468	19.72713517579778	38.402817551568205	15.38583188722955
9	14.992605636812817	18.541472546823844	39.57480274574504	26.89111907061831
10-14	26.96882399705935	34.60502132824988	23.0181824398834	15.407972234807362
15-19	20.783460561971605	36.70596079705251	21.042733435344203	21.467845205631683
20-24	25.586890178746973	31.0569237739462	26.161427924192814	17.19475812311401
25-29	18.837247074311215	25.159897761174893	39.15687162872603	16.845983535787862
30-34	19.301766953607853	28.667903334729573	31.20515297355981	20.82517673810277
35-39	20.639932980569494	30.181909882801484	27.856831451261314	21.32132568536771
40-44	20.341081030252774	29.019327925047655	29.484531676084153	21.15505936861542
45-49	20.541369966062863	31.34107248185603	26.273069985724177	21.84448756635693
50-54	22.02998777579265	29.957599952128977	27.665347364101862	20.34706490797651
55-59	21.301322436976942	27.03857891452458	30.166266316752292	21.493832331746184
60-64	22.174370196869578	26.00610355527821	28.72457920517007	23.094947042682147
65-69	20.181824398833996	27.100127371111547	29.458630033937133	23.259418196117316
70-74	20.949470426821453	26.032005197425224	29.58856566450962	23.429958711243707
75-79	20.58308614219403	25.849753378753814	30.510766705704345	23.056393773347807
80-84	23.368239286721774	25.282139834674005	27.594823090929292	23.75479778767492
85-89	20.877236474299245	28.422222412186592	25.933527666886075	24.767013446628088
90-94	20.489566681768835	30.458963421410314	28.652943640420236	20.39852625640061
95-99	21.810892367136546	30.763286345645874	26.352570075482344	21.07325121173524
100-104	22.636752976979167	28.895376172198905	26.06192458604389	22.40594626477804
105-109	24.05510296543883	27.29477436506783	23.593660508971542	25.056462160521793
110-114	21.33756763918927	27.749292620169086	29.681572221129926	21.231567519511714
115-119	23.39354254109642	27.70398611740368	27.068412819175762	21.83405852232414
120-124	18.161410827399322	29.257828194322155	27.948983168206805	24.63177781007172
125-129	20.694984655627838	25.378822201896035	30.174301809695592	23.751891332780538
130-134	24.884553901915694	29.162257118677392	25.416777083457998	20.536411895948913
135-139	24.1265675622537	27.242287209033943	24.576469683110933	24.054675545601423
140-144	21.32312084868483	28.87024388575923	26.388815277694665	23.41781998786128
145-149	20.86885904548602	26.84461579230815	28.823313187611667	23.463211974594163
150-154	23.3775570391773	25.63578700814662	25.259914003128713	25.72674194954736
155-159	22.230105743667774	27.325548593361315	29.47478650379121	20.969559159179695
160-164	24.825398996418222	28.64661782682658	25.974474487309905	20.553508689445295
165-169	19.897077303151793	25.043896017301954	28.29681743189065	26.762209247655598
170-174	22.84336772638292	28.428719193715217	26.49438797753481	22.233525102367054
175-179	20.77627990870312	29.058821518024295	28.22270283208384	21.94219574118874
180-184	20.163530829792872	29.16097485916516	26.394029799711067	24.2814645113309
185-189	22.841829014968244	27.204503295406944	27.453860028551645	22.499807661073167
190-194	21.155486788452826	27.613886015677757	26.021234217522505	25.209392978346912
195-199	21.590771150870655	29.27022336960703	27.30229695420624	21.836708525316077
200-204	22.510151221138475	27.551910139253383	27.203990391602055	22.733948248006087
205-209	22.273788051051024	26.90830134808217	27.234166232123165	23.58374436874364
210-214	23.263606910523933	27.347774424906607	26.659286550807398	22.729332113762062
215-219	23.64751540848514	26.865131944503805	26.948393328831177	22.538959318179874
220-224	20.516750583428077	26.588505825732383	27.87196211350561	25.02278147733393
225-229	22.237628332806185	28.024465511493318	26.172711807900427	23.56519434780007
230-234	22.47877860507262	25.845222728477275	28.000700968533355	23.675297697916754
235-239	22.477581829527875	26.827604482779254	25.329925372496387	25.36488831519648
240-244	23.63144442259854	30.93946880262607	22.550585137757416	22.87850163701798
245-249	21.719253553995948	29.20542652225575	23.55074755729563	25.52457236645267
250-254	22.98210820560604	27.14073225566545	23.951068977013364	25.92609056171515
255-259	22.79045315051162	27.53147947102521	26.05166650994606	23.62640086851711
260-264	22.65436267428044	28.50172250194476	25.363007667911887	23.48090715586292
265-269	21.584530821244478	28.22663509458801	25.609030526324787	24.579803557842727
270-274	21.180106171087612	29.943495097494466	23.492020071635565	25.384378659782357
275-279	22.312597772287805	28.93384395756576	22.99381950915106	25.759738760995376
280-284	19.843051435703234	29.711662577683555	25.973106743830193	24.472179242783014
285-289	21.766953607850848	28.464707944025097	26.082611706174507	23.685726741949548
290-294	21.099836725622108	30.25209222010412	24.11930142501774	24.528769629256033
295-299	20.490763457313584	31.682580931946212	21.763790701054017	26.06286490968619
300-301	16.79995041929886	32.90384763337636	28.994024670673014	21.302177276651765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.0
21	0.25
22	0.0
23	0.0
24	0.25
25	0.5
26	0.0
27	0.25
28	0.75
29	0.75
30	1.5
31	2.0
32	1.25
33	6.0
34	19.0
35	74.25
36	236.5
37	662.75
38	1308.75
39	1974.5
40	2960.25
41	4061.5
42	5632.75
43	7854.5
44	9821.75
45	9903.0
46	8453.0
47	6750.0
48	5361.25
49	4249.0
50	3385.75
51	2481.0
52	1508.75
53	695.5
54	284.5
55	121.5
56	55.0
57	32.0
58	25.0
59	17.0
60	14.25
61	12.25
62	9.0
63	6.75
64	3.75
65	2.75
66	2.0
67	1.75
68	3.25
69	2.25
70	1.25
71	1.75
72	1.75
73	1.75
74	0.25
75	1.0
76	1.5
77	1.0
78	0.75
79	0.25
80	0.75
81	0.75
82	0.5
83	1.0
84	1.0
85	0.5
86	0.25
87	0.0
88	0.0
89	0.75
90	1.0
91	0.5
92	1.0
93	0.75
94	0.0
95	0.25
96	0.75
97	0.0
98	0.25
99	0.25
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	233962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.66174527638922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.31567886752116	8.35677467737591
2	7.379096881833299	2.6065545899347535
3	7.179260626306292	3.8039481736389678
4	6.684287364529018	4.722247231459941
5	5.620811214451236	4.963666795815482
6	5.13454618248711	5.441102807096641
7	4.26506284422548	5.272991747975177
8	3.146424546893728	4.44570791028923
9	2.3173801482973837	3.6836080099010506
>10	10.806323176823987	29.6569426852127
>50	0.06884174140850019	0.7801037467442209
>100	0.0374077558686262	1.5365714523433878
>500	0.007479774892361617	0.8010019789037306
>1k	0.02991909956944646	14.22163821367503
>5k	0.007479774892361615	9.707139979633775
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9048	3.8672946888811004	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7198	3.0765679896735367	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5798	2.4781802173002454	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4942	2.1123088364777187	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4455	1.904155375659295	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4321	1.846881117446423	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3315	1.416896761012472	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2719	1.1621545379164138	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2636	1.1266786914114257	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2537	1.084364127507886	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1771	0.7569605320522136	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1750	0.7479847154666143	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1632	0.6975491746522939	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1151	0.49196023285832735	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1067	0.45605696651593	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	650	0.277822894316171	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	606	0.2590164214701533	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	563	0.24063736846154504	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	477	0.20387926244432855	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	443	0.1893469879724058	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	383	0.16370179772783613	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	368	0.15729050016669374	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	238	0.10172592130345953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	4.2741983740949386E-4	0.0	0.0
8	4.2741983740949386E-4	0.0	0.0
9	4.2741983740949386E-4	0.0	0.0
10-14	0.001453227447192279	0.0	0.0
15-19	0.0017096793496379757	0.0	0.0
20-24	0.002137099187047469	0.0	0.0
25-29	0.002137099187047469	0.0	0.0
30-34	0.0031629067968302546	0.0	0.0
35-39	0.004017746471649242	0.0	0.0
40-44	0.004616134244022534	0.0	0.0
45-49	0.006154845658696712	0.0	0.0
50-54	0.009488720390490764	0.0	0.0
55-59	0.011625819577538232	0.0	0.0
60-64	0.016925825561415954	0.0	0.0
65-69	0.027269385626725706	0.0	0.0
70-74	0.04291295167591318	0.0	0.0
75-79	0.06402749164394218	0.0	0.0
80-84	0.09540010770979904	0.0	0.0
85-89	0.14121951428009677	0.0	0.0
90-94	0.2106324958753986	0.0	0.0
95-99	0.2865422589993247	0.0	0.0
100-104	0.3772407484976193	0.0	0.0
105-109	0.4924731366632188	0.0	0.0
110-114	0.6549781588463084	0.0	0.0
115-119	0.8373154614851984	0.0	0.0
120-124	1.0560689342713776	0.0	0.0
125-129	1.3109821253023997	0.0	0.0
130-134	1.6052179413750949	0.0	0.0
135-139	1.909284413708209	0.0	0.0
140-144	2.2319009069848947	0.0	0.0
145-149	2.627777160393568	0.0	0.0
150-154	3.067506689120455	0.0	0.0
155-159	3.5478411024012444	0.0	0.0
160-164	4.0588642600080345	0.0	0.0
165-169	4.608269719014198	0.0	0.0
170-174	5.180926817175439	0.0	0.0
175-179	5.773758131662407	0.0	0.0
180-184	6.404458843743856	0.0	0.0
185-189	7.068583787110728	0.0	0.0
190-194	7.770834579974526	0.0	0.0
195-199	8.402903035535687	0.0	0.0
200-204	9.013771467161334	0.0	0.0
205-209	9.607201169420676	0.0	0.0
210-214	10.237559945632196	0.0	0.0
215-219	10.848513861225328	0.0	0.0
220-224	11.437241945273163	0.0	0.0
225-229	12.037766816833502	0.0	0.0
230-234	12.577085167676803	0.0	0.0
235-239	13.054513126063208	0.0	0.0
240-244	13.455090997683381	0.0	0.0
245-249	13.776938135252731	0.0	0.0
250-254	14.072114274967731	0.0	0.0
255-259	14.295996785802823	0.0	0.0
260-264	14.497226045255212	0.0	0.0
265-269	14.660500423145638	0.0	0.0
270-274	14.786247339311512	0.0	0.0
275-279	14.872928082338158	0.0	0.0
280-284	14.932681375608004	0.0	0.0
285-289	14.976620134893698	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4185	0.0	290.7706	1
GGGTTAT	4265	0.0	285.66235	2
GTTATTA	4245	0.0	284.2285	4
GGTTATT	4300	0.0	282.99417	3
GGGGGGA	2760	0.0	280.57065	1
TTAGTAG	4395	0.0	278.55518	8
TTATTAG	4350	0.0	276.68967	5
TAGTAGA	4430	0.0	275.02258	9
GGGGAGC	2835	0.0	272.62787	3
TATTAGT	4430	0.0	272.02597	6
ATTAGTA	4460	0.0	270.85764	7
GGAGCGA	2150	0.0	268.24417	5
GGGAGCG	2195	0.0	264.76083	4
CGAAAGC	2250	0.0	257.63333	9
GAGCGAA	2305	0.0	249.56616	6
AGCGAAA	2320	0.0	247.31682	7
GCGAAAG	2420	0.0	240.75414	8
GGGGGAG	3395	0.0	228.96172	2
GGGAGCA	940	0.0	197.71275	4
CAAAAGC	1115	0.0	138.90135	9
>>END_MODULE