##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30191_flu.3520000003b9d4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280371
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12627197534695	34.0	34.0	34.0	34.0	34.0
2	33.45276793962286	34.0	34.0	34.0	34.0	34.0
3	33.43291923915098	34.0	34.0	34.0	34.0	34.0
4	33.46622867557629	34.0	34.0	34.0	34.0	34.0
5	33.451508893573155	34.0	34.0	34.0	34.0	34.0
6	36.997214405198825	38.0	38.0	38.0	36.0	38.0
7	36.16316951467876	38.0	38.0	38.0	34.0	38.0
8	36.915237310563505	38.0	38.0	38.0	36.0	38.0
9	36.97308209479582	38.0	38.0	38.0	36.0	38.0
10-14	36.994657079369844	38.0	38.0	38.0	36.4	38.0
15-19	36.90278809149306	38.0	38.0	38.0	36.6	38.0
20-24	36.999091917495036	38.0	38.0	38.0	36.8	38.0
25-29	36.92995780590717	38.0	38.0	38.0	36.0	38.0
30-34	36.60418730895849	38.0	38.0	38.0	34.6	38.0
35-39	36.876521466200145	38.0	38.0	38.0	36.0	38.0
40-44	36.421546450952484	38.0	37.6	38.0	34.2	38.0
45-49	36.7361282015615	38.0	38.0	38.0	35.2	38.0
50-54	36.6919931091304	38.0	38.0	38.0	35.2	38.0
55-59	36.36436507342057	38.0	37.4	38.0	33.8	38.0
60-64	36.55394744820256	38.0	37.6	38.0	34.2	38.0
65-69	36.42297027866648	38.0	37.4	38.0	34.0	38.0
70-74	36.28436250539464	38.0	37.0	38.0	34.0	38.0
75-79	36.33112055098423	38.0	37.0	38.0	34.0	38.0
80-84	35.646686711535786	38.0	37.0	38.0	30.2	38.0
85-89	35.871500262152644	38.0	37.0	38.0	32.0	38.0
90-94	35.87960737736785	38.0	37.0	38.0	32.0	38.0
95-99	34.99080860716693	38.0	36.0	38.0	27.0	38.0
100-104	35.32592600518599	38.0	36.0	38.0	28.4	38.0
105-109	35.1557093993316	38.0	35.8	38.0	27.4	38.0
110-114	34.72536389284198	38.0	35.2	38.0	24.2	38.0
115-119	34.69987480873557	38.0	35.0	38.0	24.4	38.0
120-124	34.27283278227777	37.6	34.6	38.0	24.0	38.0
125-129	33.86083938781115	37.0	34.0	38.0	23.0	38.0
130-134	33.72063515841511	37.0	34.0	38.0	23.0	38.0
135-139	33.12162598842249	37.0	31.6	38.0	21.6	38.0
140-144	31.813988607951607	36.2	28.4	38.0	17.4	38.0
145-149	31.742723034835983	35.4	28.2	37.6	18.2	38.0
150-154	30.711375998230913	35.0	26.2	37.0	14.2	38.0
155-159	30.513852003238565	35.0	26.0	37.0	15.0	37.4
160-164	29.856530097620652	35.0	24.4	37.0	11.0	37.4
165-169	28.750293004626013	33.4	23.0	36.6	10.0	37.0
170-174	28.131853151716832	32.6	21.8	36.2	9.6	37.0
175-179	27.220392979302424	30.4	20.2	36.0	9.2	37.0
180-184	26.357128233661832	28.6	20.0	35.0	9.0	37.0
185-189	25.695452097399517	27.8	19.6	35.0	9.0	36.8
190-194	24.68308847919364	25.8	17.0	34.8	8.6	36.0
195-199	23.81288079009598	24.8	15.4	34.0	8.0	36.0
200-204	22.901829361809888	23.6	14.6	32.4	8.0	36.0
205-209	22.050925381013013	22.8	11.8	31.4	8.0	35.6
210-214	20.715972051317717	20.4	9.0	29.2	8.0	35.0
215-219	20.18526523784557	20.0	9.0	28.0	8.0	34.4
220-224	19.498382500329917	19.6	9.0	27.2	8.0	34.0
225-229	18.745517189723618	18.0	9.0	26.0	8.0	33.0
230-234	17.81533396820641	16.4	9.0	25.0	8.0	31.6
235-239	16.758451480359952	15.0	9.0	23.4	7.6	30.2
240-244	15.90449725542228	14.0	8.0	22.0	7.0	28.8
245-249	15.205535522575445	13.2	8.0	20.8	7.0	27.4
250-254	14.511320357668945	12.4	8.0	19.6	7.0	26.0
255-259	13.842789018835756	11.4	8.0	18.4	7.0	25.0
260-264	13.231615965987922	10.6	8.0	17.2	7.0	23.8
265-269	12.814229717053475	9.8	8.0	16.0	7.0	22.8
270-274	12.320595211344967	9.0	7.6	15.4	7.0	21.6
275-279	11.834286712962466	8.6	7.0	14.0	7.0	21.0
280-284	11.646240873699492	8.4	7.0	13.8	7.0	20.6
285-289	11.286260704566448	8.0	7.0	13.0	7.0	19.0
290-294	11.049971644713612	8.0	7.0	13.0	7.0	19.0
295-299	10.753570804398457	8.0	7.0	13.0	7.0	18.4
300-301	10.42957367202742	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.23422253086324218
1101	2	0.11937894935886817
1101	3	0.21219402447864155
1101	4	0.2638383583307373
1101	5	0.25221854320912485
1101	6	0.31675673990758213
1101	7	1.1214908451936196
1101	8	0.358085001164433
1101	9	0.1877990033922643
1101	10-14	0.21378272435516976
1101	15-19	0.29068303233741943
1101	20-24	0.28254772703023434
1101	25-29	0.25878559458207206
1101	30-34	0.5678425344138986
1101	35-39	0.41423038509616816
1101	40-44	0.7251231514380123
1101	45-49	0.4249576125044996
1101	50-54	0.39448168904132075
1101	55-59	0.5066273340692504
1101	60-64	0.49781346551849737
1101	65-69	0.5304863929421941
1101	70-74	0.51183752829958
1101	75-79	0.6774614097828504
1101	80-84	0.878586266814608
1101	85-89	0.6519743139256988
1101	90-94	0.5681257058553584
1101	95-99	1.2172081991206127
1101	100-104	0.5984081053366168
1101	105-109	0.9126812087463421
1101	110-114	0.8610431503021658
1101	115-119	0.7663024398576042
1101	120-124	1.0588886304733407
1101	125-129	0.9417495471357
1101	130-134	1.0224846283096198
1101	135-139	1.3225369424922633
1101	140-144	1.7597300681465455
1101	145-149	1.7728838235991908
1101	150-154	1.5465898019177402
1101	155-159	1.043341922074191
1101	160-164	1.498911716380718
1101	165-169	1.4435318042058896
1101	170-174	1.3900951308340765
1101	175-179	1.327966934182765
1101	180-184	0.9289250556597963
1101	185-189	1.232373917616819
1101	190-194	1.2185865146058994
1101	195-199	1.088823705433441
1101	200-204	1.455919234055063
1101	205-209	1.2083046632758787
1101	210-214	1.5579084678122648
1101	215-219	1.6493113042898173
1101	220-224	1.3279046720256993
1101	225-229	0.9894172383360846
1101	230-234	0.5966969249995273
1101	235-239	0.47275280748872106
1101	240-244	0.8535966143462854
1101	245-249	0.683722542431008
1101	250-254	0.5642871689832472
1101	255-259	0.31330952550900726
1101	260-264	0.1559338731997464
1101	265-269	0.22763467374648982
1101	270-274	0.07121303433317827
1101	275-279	0.175301220205764
1101	280-284	0.2836288142677521
1101	285-289	0.1381213112794537
1101	290-294	-0.006222005213347259
1101	295-299	0.032389153061208376
1101	300-301	0.08020965131129287
1102	1	-0.10841486117222132
1102	2	-0.049267385579604195
1102	3	0.0344213423720916
1102	4	-0.05043015806458584
1102	5	0.10591800052013411
1102	6	-0.02825205012528187
1102	7	0.9105205020001605
1102	8	0.04945930369443374
1102	9	0.10928490112213751
1102	10-14	0.1159420150377315
1102	15-19	0.15795697562260358
1102	20-24	0.1116320349106843
1102	25-29	0.1235394736617863
1102	30-34	0.43081471204900623
1102	35-39	0.21880655264183702
1102	40-44	0.47180166554028347
1102	45-49	0.0761572609031802
1102	50-54	0.014165630033446064
1102	55-59	0.294077460187097
1102	60-64	0.15772441866379694
1102	65-69	0.17191458219542
1102	70-74	0.20011735708502698
1102	75-79	0.2843432411670861
1102	80-84	0.6968848221918194
1102	85-89	0.3851073873798043
1102	90-94	0.3055486975239319
1102	95-99	1.043628744866993
1102	100-104	0.8235617398241217
1102	105-109	0.8795894102291726
1102	110-114	0.6506399553163078
1102	115-119	0.6707368203513724
1102	120-124	1.11080447546302
1102	125-129	0.7991045115933915
1102	130-134	0.899420346417088
1102	135-139	1.1377673942235162
1102	140-144	1.9657321318933896
1102	145-149	1.75646442437883
1102	150-154	1.5236818297400987
1102	155-159	1.022304698195878
1102	160-164	1.4853215612175248
1102	165-169	1.3855966975789755
1102	170-174	1.6680038673623976
1102	175-179	1.4557342658345291
1102	180-184	1.2543297793470245
1102	185-189	1.0805960379256163
1102	190-194	1.7071074960321724
1102	195-199	1.7167744812493844
1102	200-204	1.6452175601270653
1102	205-209	1.132979049884458
1102	210-214	1.4444283791475847
1102	215-219	1.5647767938564314
1102	220-224	1.1217180175886412
1102	225-229	1.199937761151947
1102	230-234	1.3201861858724193
1102	235-239	0.698867230786135
1102	240-244	1.0147160059231464
1102	245-249	0.31163013243329907
1102	250-254	1.2282453590008284
1102	255-259	0.4844793642310137
1102	260-264	0.432574551943155
1102	265-269	0.39498963820418354
1102	270-274	0.31932508814346505
1102	275-279	0.3755996991479016
1102	280-284	0.43563561698884
1102	285-289	0.34803188224941906
1102	290-294	0.1911727054799197
1102	295-299	0.10377071692270867
1102	300-301	-0.13555699242485275
1103	1	0.013850265862259903
1103	2	0.04164305288946224
1103	3	0.10477205833070258
1103	4	0.08805891726671433
1103	5	-0.037799944397001184
1103	6	0.08146156642921909
1103	7	0.9291569195389258
1103	8	0.3036542578908694
1103	9	0.12566449259585966
1103	10-14	0.04974919537630029
1103	15-19	0.05176608297192331
1103	20-24	-0.0012359500289846892
1103	25-29	0.06313287439109416
1103	30-34	0.4345494071366005
1103	35-39	-0.023841160144613127
1103	40-44	0.30212169755830587
1103	45-49	-0.0020267538013882813
1103	50-54	0.06332738035214902
1103	55-59	0.18517022352053658
1103	60-64	0.11594460187804145
1103	65-69	0.3461897296858538
1103	70-74	0.1893372141897629
1103	75-79	0.21004734125305902
1103	80-84	0.6883111066186061
1103	85-89	0.27098388081314084
1103	90-94	0.1944513390109961
1103	95-99	0.9127076337229525
1103	100-104	0.5428548233378052
1103	105-109	0.7004930300447825
1103	110-114	0.5064350696028725
1103	115-119	0.41860154419590856
1103	120-124	0.7491734832007069
1103	125-129	0.9055533272229255
1103	130-134	0.8481070144672529
1103	135-139	0.8995236421853292
1103	140-144	1.4730896072128203
1103	145-149	1.598069327880058
1103	150-154	1.8865772375252305
1103	155-159	0.5831552510997398
1103	160-164	1.0881942895682997
1103	165-169	1.2298771814068985
1103	170-174	1.6369671355726432
1103	175-179	1.7820957730534062
1103	180-184	1.0892309088717944
1103	185-189	1.3577906067198988
1103	190-194	1.1185509753242222
1103	195-199	1.5179781218174107
1103	200-204	1.4052854501985124
1103	205-209	0.9331487750630743
1103	210-214	1.2784860708851582
1103	215-219	1.4363674671243452
1103	220-224	1.4186763052172324
1103	225-229	0.17504967600772048
1103	230-234	1.6606489649183978
1103	235-239	1.4260595761064607
1103	240-244	0.6178221990392654
1103	245-249	0.8725481307138168
1103	250-254	0.7440520353519613
1103	255-259	0.6583320516698681
1103	260-264	0.1968729717943294
1103	265-269	0.63824584169652
1103	270-274	0.5874982819414551
1103	275-279	0.4140709866875554
1103	280-284	0.5331949837434582
1103	285-289	0.3633768171757463
1103	290-294	0.039233095032555454
1103	295-299	-0.0528663528350819
1103	300-301	-0.09112827699987314
2116	1	0.2093258604224033
2116	2	0.11227797635802972
2116	3	0.12896148924532724
2116	4	0.23836543573815305
2116	5	0.28096855471575566
2116	6	0.44763777973894037
2116	7	1.144485699427598
2116	8	0.38773529159183084
2116	9	0.490182257099697
2116	10-14	0.284433447431951
2116	15-19	0.45160577220467957
2116	20-24	0.3366377365116975
2116	25-29	0.265538607018442
2116	30-34	0.6162425601887591
2116	35-39	0.2320198229281374
2116	40-44	0.7206670469393828
2116	45-49	0.3289749921221059
2116	50-54	0.4189530743478187
2116	55-59	0.6865262598100372
2116	60-64	0.5183214143001678
2116	65-69	0.4785250184059109
2116	70-74	0.7204025131568486
2116	75-79	0.5933171810494073
2116	80-84	1.0341246391324148
2116	85-89	0.6400449738540814
2116	90-94	0.6390570066571399
2116	95-99	1.5023734803887336
2116	100-104	0.587309820285121
2116	105-109	0.807477081087157
2116	110-114	0.9651102385718389
2116	115-119	0.9866925607232631
2116	120-124	1.3016458037079914
2116	125-129	1.111841508142021
2116	130-134	1.1031157440847537
2116	135-139	1.60691793169444
2116	140-144	2.2866528264244153
2116	145-149	1.8324215331349727
2116	150-154	2.505373228684938
2116	155-159	2.467764703365333
2116	160-164	2.0904575634069396
2116	165-169	2.0378130999447563
2116	170-174	1.8446409870656026
2116	175-179	2.5206549755698155
2116	180-184	2.2706234346054224
2116	185-189	1.850545559751346
2116	190-194	1.7541099743280775
2116	195-199	1.324653333631133
2116	200-204	1.4146577389292716
2116	205-209	0.9778244224650763
2116	210-214	2.278235072066959
2116	215-219	1.7309583176283034
2116	220-224	1.359334900409337
2116	225-229	0.9447645085026046
2116	230-234	1.4019509454168961
2116	235-239	1.0254401124513066
2116	240-244	0.6295413088682071
2116	245-249	0.7132406446651345
2116	250-254	1.0209940628368646
2116	255-259	1.3878231631697542
2116	260-264	0.4751153373957102
2116	265-269	0.5203788674997689
2116	270-274	0.46182178121487993
2116	275-279	0.5972197900694329
2116	280-284	0.30975605000643114
2116	285-289	0.150557311389921
2116	290-294	0.46879240109044673
2116	295-299	0.16333610288308442
2116	300-301	-0.056314241060269055
1117	1	0.025691699175730776
1117	2	0.009087090679912535
1117	3	0.14009066700661776
1117	4	0.04965139322211343
1117	5	0.12292295400570197
1117	6	0.15831917133176887
1117	7	1.0848439283623748
1117	8	0.16258906085282376
1117	9	0.11762125487349806
1117	10-14	0.1716113396722676
1117	15-19	0.21201943786737587
1117	20-24	0.27191353787070227
1117	25-29	0.3238777604806842
1117	30-34	0.4605537479315629
1117	35-39	0.2588935406809867
1117	40-44	0.5007825764983522
1117	45-49	0.27442656137436217
1117	50-54	0.23492496312515243
1117	55-59	0.41472805822987624
1117	60-64	0.36246345454635787
1117	65-69	0.5378805118800898
1117	70-74	0.4568528892826791
1117	75-79	0.6205248286988052
1117	80-84	0.7973650686579319
1117	85-89	0.5004480905331263
1117	90-94	0.47268352898461075
1117	95-99	1.0711175693203003
1117	100-104	0.6762257485229313
1117	105-109	0.7165412043574833
1117	110-114	0.7503409339317457
1117	115-119	0.8736998504347397
1117	120-124	1.1553193963280677
1117	125-129	1.0089812785379308
1117	130-134	1.0048359559374376
1117	135-139	1.2635376008201078
1117	140-144	1.5380107352521009
1117	145-149	1.7517767356027072
1117	150-154	2.341774133714978
1117	155-159	2.2205045290524694
1117	160-164	2.1294800727555234
1117	165-169	1.526248503788949
1117	170-174	1.4521194889644278
1117	175-179	1.7324589245272897
1117	180-184	1.7079693830681784
1117	185-189	1.3807138342286223
1117	190-194	-1.092110215699126
1117	195-199	1.245713106355101
1117	200-204	1.5557074719308588
1117	205-209	1.4889081304692091
1117	210-214	1.9968507544043241
1117	215-219	1.637898093844349
1117	220-224	1.718510333645181
1117	225-229	1.6355243263325683
1117	230-234	1.4044739713019219
1117	235-239	1.037915195024386
1117	240-244	0.8951316154435016
1117	245-249	1.041761822659229
1117	250-254	0.654864303062686
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2108	240-244	-0.6865888146640291
2108	245-249	-1.0283994676633537
2108	250-254	-1.0206733313459164
2108	255-259	-0.6568550564528088
2108	260-264	-0.395451906918753
2108	265-269	-0.4510475733458943
2108	270-274	-0.27215431260559697
2108	275-279	-0.39379138457808516
2108	280-284	-0.9487729851616962
2108	285-289	-1.2038437973446392
2108	290-294	-0.8362889352111527
2108	295-299	-0.5095704694974916
2108	300-301	2.5314796754499813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	63.0
11	338.0
12	529.0
13	508.0
14	583.0
15	879.0
16	1327.0
17	1919.0
18	2578.0
19	2808.0
20	3693.0
21	4796.0
22	6753.0
23	9542.0
24	13502.0
25	19324.0
26	27598.0
27	37485.0
28	46041.0
29	46059.0
30	33491.0
31	16001.0
32	4084.0
33	452.0
34	14.0
35	2.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.589975425418462	22.858997542541847	14.690178370801545	33.860848661238144
2	43.22094653155997	25.0710665511055	17.335601756244404	14.372385161090126
3	42.22904651336978	24.1084134949763	19.305491652132353	14.357048339521564
4	42.73516162513241	22.804070321110242	19.32938856015779	15.131379493599553
5	27.58024189377646	22.836170645323516	34.946552960184896	14.637034500715124
6	26.452093832814377	22.177757328682354	35.302866558952246	16.067282279551023
7	17.259987659208694	46.50088632561856	20.543137485688607	15.695988529484147
8	25.908528342802928	20.901234435801136	36.6032863598589	16.586950861537034
9	15.730228875311642	19.63612499152908	37.87588587978072	26.75776025337856
10-14	26.00575665814225	33.70384240880833	23.819367908949214	16.47103302410021
15-19	21.17301718080686	34.88862970849339	22.20778896533522	21.730564145364532
20-24	24.9435925969519	30.614364538415174	26.48868820241751	17.95335466221542
25-29	19.29165284569374	25.806877316127558	37.24943021924522	17.652039618933486
30-34	19.608304710544246	28.26597615302581	31.05371097581419	21.072008160615756
35-39	20.610904836805517	29.91479147272721	28.073374207746166	21.40092948272111
40-44	20.450189213577723	28.6030295572652	29.337484975264914	21.609296253892165
45-49	20.87648151913001	30.311266143788053	26.63228365273156	22.179968684350378
50-54	21.90362055990099	29.229199881585473	27.74388221321035	21.12329734530319
55-59	21.405423528110965	26.854988568717875	29.806649047155375	21.93293885601578
60-64	21.608226243085056	26.598328643119295	28.4942451252091	23.29919998858655
65-69	20.107072414764723	27.261735343526972	29.446768745697664	23.18442349601064
70-74	20.996822067902887	26.515224470433818	29.29504121324959	23.19291224841371
75-79	20.50654311608547	26.10555299941863	30.410277810472554	22.97762607402335
80-84	23.536956389926207	25.29391413519943	27.686743636110723	23.48238583876364
85-89	20.660053999878734	28.538400904515804	26.3091403889846	24.49240470662087
90-94	20.265719350432107	30.398793027809578	28.61658302748858	20.718904594269734
95-99	21.842344607680538	30.122088233091155	26.508590403429743	21.526976755798565
100-104	22.69471521662369	28.698189185044104	26.289309522026173	22.31778607630604
105-109	23.54045175856276	27.601642109918643	24.138302463521548	24.71960366799705
110-114	21.550017655178316	27.766566442321068	29.14752238997614	21.535893512524478
115-119	23.274518405969232	27.82777106048771	26.8816675048418	22.016043028701258
120-124	19.22723819510577	28.310131932332517	28.229881121799334	24.232748750762383
125-129	20.971997817177954	25.20303455064896	30.299924029232695	23.52504360294039
130-134	24.769394837554525	28.605882919417486	25.735329260158863	20.889392982869126
135-139	23.779777509086173	27.59279668724654	25.01000460104647	23.617421202620815
140-144	21.23693249301818	29.151231760774117	26.46286527493928	23.148970471268427
145-149	20.564038363454138	27.367737747484583	28.812537673297168	23.25568621576411
150-154	23.159171240962866	25.64580502263073	25.841617000331702	25.353406736074703
155-159	22.179398011919922	27.19468133294813	29.238473308580414	21.387447346551532
160-164	24.542695214555	28.72964750277311	26.352368825591803	20.37528845708008
165-169	20.436564409300534	25.605358614122004	28.369053860777328	25.589023115800135
170-174	22.309368657956778	28.630493167980998	26.724875254573405	22.335262919488823
175-179	21.265608782648705	28.500165851675103	28.11959867461328	22.114626691062913
180-184	20.387343912173513	28.983596734327016	26.859125943838702	23.76993340966077
185-189	22.670176302113983	27.189616615127814	27.706717171176763	22.43348991158144
190-194	20.756711642787593	27.639877162759348	27.355610958337344	24.24780023611572
195-199	21.696609135752272	29.185044102278766	27.502345107018915	21.616001654950047
200-204	22.923412193129817	27.51753926047986	27.409753505177072	22.14929504121325
205-209	22.1458710066305	27.28327822777677	27.89033102567669	22.68051973991604
210-214	23.770076077768383	27.261450007311737	27.30489244608037	21.663581468839503
215-219	23.508137432188065	27.2100894885705	27.951392975735722	21.33038010350571
220-224	20.6832375673661	27.295333682870197	27.911873909926488	24.109554839837216
225-229	22.612823722852934	28.198636806231743	26.340170702390758	22.848368768524562
230-234	22.458528164467793	26.98481654664712	27.968441814595664	22.58821347428942
235-239	22.82532786914481	27.43636110724718	25.967878275570584	23.770432748037422
240-244	24.247728902061912	30.779288870817595	23.247839469845314	21.725142757275183
245-249	22.519304778311593	29.33983899904056	24.158204664533777	23.98265155811407
250-254	23.317390172307405	27.829197741563856	24.630578768845567	24.222833317283172
255-259	23.57454943628264	28.07843892556648	26.143574050097907	22.203437588052974
260-264	23.465479668011312	28.984381408918896	25.431089520670824	22.119049402398964
265-269	22.57779870243356	28.713098002289822	25.841188996008857	22.867914299267756
270-274	22.127894825071067	30.45322091086453	24.030160037949717	23.388724226114686
275-279	23.08084644988248	29.841602733520944	23.003449001501583	24.074101815095002
280-284	21.0263543661791	30.386452236500922	25.843186349515463	22.744007047804516
285-289	22.653341465415465	29.608411711624953	25.365676193329552	22.372570629630026
290-294	22.064264849074974	31.233330123300913	23.995705689960804	22.70669933766331
295-299	21.464416790609587	32.706521002528795	21.940999604096	23.888062602765622
300-301	17.573322490557157	34.618059642402386	28.925423813447182	18.883194053593275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.25
13	0.25
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.25
25	0.75
26	0.75
27	0.75
28	1.25
29	1.25
30	1.5
31	1.5
32	3.75
33	6.5
34	24.75
35	83.25
36	304.5
37	865.75
38	1767.75
39	3017.5
40	4373.75
41	6205.25
42	8771.75
43	10938.75
44	11748.75
45	10410.5
46	8101.25
47	6406.75
48	5261.0
49	4522.75
50	3793.75
51	2867.25
52	1679.5
53	830.5
54	377.0
55	193.0
56	135.25
57	114.75
58	108.0
59	102.5
60	95.25
61	92.5
62	82.75
63	61.75
64	39.0
65	27.75
66	18.5
67	10.75
68	9.75
69	8.0
70	3.75
71	2.0
72	2.5
73	2.25
74	2.5
75	3.5
76	1.75
77	1.0
78	1.25
79	0.75
80	1.75
81	1.0
82	0.25
83	0.25
84	0.75
85	0.75
86	1.25
87	0.75
88	0.75
89	0.75
90	1.25
91	0.5
92	0.75
93	0.5
94	0.25
95	0.25
96	0.0
97	0.75
98	0.0
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	280371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.442941127198107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.22498455737041	7.9296058193687955
2	7.05460900108649	2.3199704096053404
3	5.014252390115568	2.473471705427481
4	4.461293783534276	2.9342716413515597
5	4.560508289019822	3.7494084653225954
6	4.155596360742329	4.09981357848509
7	3.9326301250799527	4.526480393521478
8	3.365949731202077	4.427689061381124
9	2.932697321634378	4.339995246317309
>10	16.15123041549683	40.27588437028973
>50	0.07718561883062842	0.856688541644308
>100	0.026748021376956463	1.1188287259629826
>500	0.00890829147589949	1.0506171293413726
>1k	0.02449780155854644	9.924651599604358
>5k	0.00890829147583507	9.972623312376411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8846	3.1551051998958526	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7846	2.7984349308594685	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5511	1.9656098526595118	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5030	1.7940514532530112	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4656	1.6606567726334038	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3636	1.296853098216292	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3284	1.171305163515485	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3040	1.084277617870607	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2328	0.8303283863167019	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2328	0.8303283863167019	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1892	0.6748201490168384	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1789	0.6380831113060909	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1493	0.5325087116713212	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1434	0.5114651657981746	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1222	0.4358510687624612	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	801	0.28569288549814353	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	795	0.2835528638839252	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	768	0.27392276661994286	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	505	0.1801184858633739	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	497	0.17726512371108283	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	402	0.14338144815262632	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	393	0.14017141573129888	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	382	0.13624804277189867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.5667026903638395E-4	0.0	0.0
2	7.133405380727679E-4	0.0	0.0
3	7.133405380727679E-4	0.0	0.0
4	7.133405380727679E-4	0.0	0.0
5	7.133405380727679E-4	0.0	0.0
6	7.133405380727679E-4	0.0	0.0
7	7.133405380727679E-4	0.0	0.0
8	0.0010700108071091518	0.0	0.0
9	0.0014266810761455358	0.0	0.0
10-14	0.0034953686365565622	0.0	0.0
15-19	0.004066041067014777	0.0	0.0
20-24	0.004993383766509375	0.0	0.0
25-29	0.004993383766509375	0.0	0.0
30-34	0.0060633945736185265	0.0	0.0
35-39	0.006634067004076741	0.0	0.0
40-44	0.008132082134029554	0.0	0.0
45-49	0.010985444286320626	0.0	0.0
50-54	0.017904847505626473	0.0	0.0
55-59	0.02575159342442692	0.0	0.0
60-64	0.03509635447318018	0.0	0.0
65-69	0.048079152266104555	0.0	0.0
70-74	0.07112005164585496	0.0	0.0
75-79	0.09929700289972929	0.0	0.0
80-84	0.14331011409881905	0.0	0.0
85-89	0.2057987452339935	0.0	0.0
90-94	0.2890455860270855	0.0	0.0
95-99	0.38976927000296036	0.0	0.0
100-104	0.5128205128205128	0.0	0.0
105-109	0.6631213641924449	0.0	0.0
110-114	0.8269043517339526	0.0	0.0
115-119	1.023144333757771	0.0	0.0
120-124	1.2581187069989408	0.0	0.0
125-129	1.5204853569021046	0.0	0.0
130-134	1.8203737191078964	0.0	0.0
135-139	2.127823491017259	0.0	0.0
140-144	2.4646628930952206	0.0	0.0
145-149	2.850009451762129	0.0	0.0
150-154	3.2581115735935597	0.0	0.0
155-159	3.720498910372328	0.0	0.0
160-164	4.239596819927881	0.0	0.0
165-169	4.818329998466318	0.0	0.0
170-174	5.428664162841378	0.0	0.0
175-179	6.086578141105892	0.0	0.0
180-184	6.784653191663902	0.0	0.0
185-189	7.538226136083975	0.0	0.0
190-194	8.315053982045217	0.0	0.0
195-199	9.101511925270446	0.0	0.0
200-204	9.892820584154567	0.0	0.0
205-209	10.721936291556544	2.1400216142183036E-4	0.0
210-214	11.547913300591002	3.5667026903638395E-4	0.0
215-219	12.366899572352347	3.5667026903638395E-4	0.0
220-224	13.189167210588826	3.5667026903638395E-4	0.0
225-229	13.966423060872913	3.5667026903638395E-4	0.0
230-234	14.687539010810676	3.5667026903638395E-4	0.0
235-239	15.337463575048776	3.5667026903638395E-4	0.0
240-244	15.910347361175013	3.5667026903638395E-4	0.0
245-249	16.364245945550714	3.5667026903638395E-4	0.0
250-254	16.78932557218828	3.5667026903638395E-4	0.0
255-259	17.12980301101041	3.5667026903638395E-4	0.0
260-264	17.406650473836454	3.5667026903638395E-4	0.0
265-269	17.631780747652222	3.5667026903638395E-4	0.0
270-274	17.798203095184597	6.420064842654912E-4	0.0
275-279	17.913906930459998	7.133405380727679E-4	0.0
280-284	17.987737676150534	7.133405380727679E-4	0.0
285-289	18.043806242443047	7.133405380727679E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4140	0.0	291.79352	1
GTTATTA	4165	0.0	287.91718	4
GGGTTAT	4195	0.0	287.61624	2
GGTTATT	4210	0.0	284.83966	3
TTATTAG	4350	0.0	274.65515	5
TTAGTAG	4420	0.0	273.64255	8
GGGGAGC	2925	0.0	271.8034	3
TATTAGT	4435	0.0	270.72153	6
ATTAGTA	4490	0.0	267.40536	7
GGGGGGA	2985	0.0	266.83417	1
GGGAGCG	2335	0.0	264.67877	4
GGAGCGA	2325	0.0	264.5484	5
TAGTAGA	4690	0.0	258.51813	9
CGAAAGC	2400	0.0	257.51044	9
GCGAAAG	2590	0.0	238.61969	8
GAGCGAA	2610	0.0	235.66093	6
AGCGAAA	2680	0.0	229.5056	7
GGGGGAG	3520	0.0	227.5355	2
GGGAGCA	845	0.0	214.70415	4
GCGCGAA	15	0.0028485525	196.66666	6
>>END_MODULE