FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30191_flu.3520000003b9d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30191_flu.3520000003b9d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280371
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC88463.1551051998958526No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT78462.7984349308594685No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG55111.9656098526595118No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA50301.7940514532530112No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG46561.6606567726334038No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA36361.296853098216292No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT32841.171305163515485No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT30401.084277617870607No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT23280.8303283863167019No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA23280.8303283863167019No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA18920.6748201490168384No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17890.6380831113060909No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA14930.5325087116713212No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14340.5114651657981746No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12220.4358510687624612No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT8010.28569288549814353Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7950.2835528638839252No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7680.27392276661994286No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5050.1801184858633739No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4970.17726512371108283No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4020.14338144815262632No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA3930.14017141573129888No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3820.13624804277189867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41400.0291.793521
GTTATTA41650.0287.917184
GGGTTAT41950.0287.616242
GGTTATT42100.0284.839663
TTATTAG43500.0274.655155
TTAGTAG44200.0273.642558
GGGGAGC29250.0271.80343
TATTAGT44350.0270.721536
ATTAGTA44900.0267.405367
GGGGGGA29850.0266.834171
GGGAGCG23350.0264.678774
GGAGCGA23250.0264.54845
TAGTAGA46900.0258.518139
CGAAAGC24000.0257.510449
GCGAAAG25900.0238.619698
GAGCGAA26100.0235.660936
AGCGAAA26800.0229.50567
GGGGGAG35200.0227.53552
GGGAGCA8450.0214.704154
GCGCGAA150.0028485525196.666666
Produced by FastQC (version 0.11.2)