##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30112_flu.3520000003bb25.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421526
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.14939292000968	34.0	34.0	34.0	34.0	34.0
2	33.35778575936004	34.0	34.0	34.0	34.0	34.0
3	33.391003164692094	34.0	34.0	34.0	34.0	34.0
4	33.3803774856118	34.0	34.0	34.0	34.0	34.0
5	33.32097901434312	34.0	34.0	34.0	34.0	34.0
6	36.80680906990316	38.0	38.0	38.0	35.0	38.0
7	36.086580661691094	38.0	38.0	38.0	34.0	38.0
8	36.751332064926004	38.0	38.0	38.0	35.0	38.0
9	36.81135920441444	38.0	38.0	38.0	36.0	38.0
10-14	36.80706338399055	38.0	38.0	38.0	36.0	38.0
15-19	36.70450743251899	38.0	38.0	38.0	35.6	38.0
20-24	36.794325379691884	38.0	38.0	38.0	35.8	38.0
25-29	36.70250328568107	38.0	38.0	38.0	35.4	38.0
30-34	36.358144930561814	38.0	37.4	38.0	33.2	38.0
35-39	36.62716795642499	38.0	38.0	38.0	34.8	38.0
40-44	36.14208850699602	38.0	37.2	38.0	33.0	38.0
45-49	36.434863329901354	38.0	37.2	38.0	34.0	38.0
50-54	36.39109568567538	38.0	37.2	38.0	34.0	38.0
55-59	36.03155297656609	38.0	37.0	38.0	32.8	38.0
60-64	36.19674468478813	38.0	37.0	38.0	33.4	38.0
65-69	36.05248406978454	38.0	37.0	38.0	32.6	38.0
70-74	35.91020008255718	38.0	37.0	38.0	31.8	38.0
75-79	35.960650588575795	38.0	37.0	38.0	32.0	38.0
80-84	35.19609324217248	38.0	36.4	38.0	28.2	38.0
85-89	35.40045643685087	38.0	37.0	38.0	29.0	38.0
90-94	35.375170689352494	38.0	37.0	38.0	28.4	38.0
95-99	34.46806317996992	38.0	35.2	38.0	23.8	38.0
100-104	34.7591190104525	38.0	36.0	38.0	24.6	38.0
105-109	34.52616303620654	38.0	35.0	38.0	24.0	38.0
110-114	34.05107205723965	37.2	34.0	38.0	23.2	38.0
115-119	34.07551799888975	37.4	34.2	38.0	23.4	38.0
120-124	33.58647817690961	37.0	33.2	38.0	21.8	38.0
125-129	33.08926756593899	37.0	31.8	38.0	21.4	38.0
130-134	32.88760835630542	37.0	31.2	38.0	20.6	38.0
135-139	32.19170300289899	36.8	29.4	38.0	18.2	38.0
140-144	30.964633261056257	35.4	26.4	37.8	14.0	38.0
145-149	30.766276338826078	35.0	26.6	37.6	14.6	38.0
150-154	29.71937009816714	35.0	23.6	37.0	10.2	38.0
155-159	29.57213789896709	34.8	23.8	37.0	10.0	37.4
160-164	28.81013602956876	33.4	22.4	37.0	10.2	37.4
165-169	27.671274369789764	31.4	20.8	36.6	9.6	37.0
170-174	27.046974563846597	30.0	20.0	36.0	9.2	37.0
175-179	26.235391411205956	28.6	19.6	35.4	9.0	37.0
180-184	25.341380602857235	27.0	17.4	35.0	8.8	36.8
185-189	24.667329180169197	26.0	16.2	35.0	8.2	36.4
190-194	23.802637559723486	24.8	15.2	34.2	8.0	36.0
195-199	22.968607867604845	23.8	14.0	33.2	8.0	36.0
200-204	22.179099747109316	23.0	12.2	31.4	8.0	36.0
205-209	21.321595346431774	21.8	9.4	30.4	8.0	35.2
210-214	20.123465693693866	20.2	9.0	28.2	8.0	35.0
215-219	19.71029118014073	19.6	9.0	27.6	8.0	34.0
220-224	19.075256093337064	18.8	9.0	26.2	8.0	34.0
225-229	18.39991934068124	17.4	9.0	25.6	8.0	32.8
230-234	17.534600475415516	15.8	9.0	24.8	7.8	31.4
235-239	16.55721070586393	14.8	8.8	22.8	7.2	30.0
240-244	15.818273131431988	13.8	8.0	21.8	7.0	28.8
245-249	15.212922571798657	13.0	8.0	20.8	7.0	27.4
250-254	14.511110109459441	12.2	8.0	19.6	7.0	26.2
255-259	13.920001613186376	11.4	8.0	18.8	7.0	25.2
260-264	13.366011112007328	10.6	8.0	17.6	7.0	23.8
265-269	12.934347110261289	9.8	8.0	16.4	7.0	23.0
270-274	12.511005726811632	9.4	8.0	15.8	7.0	22.0
275-279	12.055268239681538	9.0	7.0	14.6	7.0	21.0
280-284	11.879314680470483	8.4	7.2	14.4	7.0	20.8
285-289	11.493404440058264	8.0	7.6	13.2	7.0	19.8
290-294	11.276510108510507	8.0	7.2	13.0	7.0	19.2
295-299	10.96979308512405	8.0	7.0	13.0	7.0	19.0
300-301	10.646032510450127	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2239652570742905
1101	2	0.06353321905037745
1101	3	0.21334795793872274
1101	4	0.1373158032073576
1101	5	0.15634816185646372
1101	6	0.35821882607206845
1101	7	1.118928128387033
1101	8	0.21768810458931398
1101	9	0.3027556154396791
1101	10-14	0.36986279763682717
1101	15-19	0.3833528017679839
1101	20-24	0.3822435383388836
1101	25-29	0.35418377469597573
1101	30-34	0.7912954754503048
1101	35-39	0.43043516933622783
1101	40-44	0.7151623686399233
1101	45-49	0.41430125023337894
1101	50-54	0.5327280572887716
1101	55-59	0.6359029648771966
1101	60-64	0.5632375491819559
1101	65-69	0.5831251934565174
1101	70-74	0.5529892754233785
1101	75-79	0.6169435866354505
1101	80-84	1.1118729359109452
1101	85-89	0.6657640010912971
1101	90-94	0.7691632350285786
1101	95-99	1.3848382936024635
1101	100-104	0.7993213020154002
1101	105-109	1.085060665491767
1101	110-114	1.0275960968586162
1101	115-119	0.9685157216560967
1101	120-124	1.2253243538124465
1101	125-129	1.1510275261378382
1101	130-134	1.2647637059710775
1101	135-139	1.0232218046861377
1101	140-144	1.8270799529197532
1101	145-149	1.870162034708585
1101	150-154	1.5541869695937862
1101	155-159	1.2846013172389092
1101	160-164	1.608524139682931
1101	165-169	1.2001882782619866
1101	170-174	1.0342650987038802
1101	175-179	1.3547577066772583
1101	180-184	1.2156968534164356
1101	185-189	1.2004449707540097
1101	190-194	1.2971719343754415
1101	195-199	1.3882998642729376
1101	200-204	1.3679164504974999
1101	205-209	1.3186702621327342
1101	210-214	1.4801792241411
1101	215-219	1.5398515078831352
1101	220-224	0.8349915049676255
1101	225-229	1.0144923244614361
1101	230-234	1.0342801221368347
1101	235-239	0.7310236834093295
1101	240-244	0.28732366702610435
1101	245-249	0.37518691022479445
1101	250-254	0.7147933464075908
1101	255-259	0.29580141197791043
1101	260-264	0.34565217054600694
1101	265-269	0.22787334143759708
1101	270-274	0.09142041694584613
1101	275-279	0.22917567753716916
1101	280-284	0.2659228961240174
1101	285-289	0.22301948839507446
1101	290-294	-0.08364086962217776
1101	295-299	0.052042470501316274
1101	300-301	-0.4045915648286389
1102	1	-0.059704947759854576
1102	2	0.07218546192320474
1102	3	0.07513151291588116
1102	4	0.013016489150942334
1102	5	0.04802821526787682
1102	6	-0.16657037506122663
1102	7	1.039593025969964
1102	8	0.2500425110312179
1102	9	0.18590882080250992
1102	10-14	0.21086334666280493
1102	15-19	0.36677526709801356
1102	20-24	0.339421205859594
1102	25-29	0.1554965229438352
1102	30-34	0.554437191935989
1102	35-39	0.2922790493909915
1102	40-44	0.628542674196602
1102	45-49	0.20394821296739707
1102	50-54	0.20066646470672111
1102	55-59	0.41819094595275175
1102	60-64	0.24101555289635712
1102	65-69	0.3837103128188346
1102	70-74	0.3505998262116705
1102	75-79	0.391373717995009
1102	80-84	0.91311265014631
1102	85-89	0.3996131477286795
1102	90-94	0.5044673005321343
1102	95-99	1.0857675709215826
1102	100-104	0.9222339865491449
1102	105-109	1.1266700572251906
1102	110-114	0.9693051266415722
1102	115-119	0.7570473482642015
1102	120-124	1.2684461087977326
1102	125-129	1.1832307809807858
1102	130-134	1.3033224449736238
1102	135-139	1.4095391959315506
1102	140-144	2.067171558922439
1102	145-149	1.8185025867169315
1102	150-154	1.8370283824206588
1102	155-159	1.3829330577190682
1102	160-164	1.6096881086663544
1102	165-169	1.784178917707802
1102	170-174	1.7480720855679266
1102	175-179	1.8879993662269214
1102	180-184	1.840321516206032
1102	185-189	1.662562516540067
1102	190-194	1.7779766980231422
1102	195-199	1.9062909714075609
1102	200-204	1.8355642469376363
1102	205-209	1.4886183757878904
1102	210-214	1.4802625947539774
1102	215-219	1.90603370995181
1102	220-224	1.6275349186892072
1102	225-229	1.5736031057050823
1102	230-234	1.554608995481285
1102	235-239	1.0005400321831708
1102	240-244	0.9221939342187504
1102	245-249	0.6959304337515739
1102	250-254	0.764262451834135
1102	255-259	0.8007546769880101
1102	260-264	0.5686853357824955
1102	265-269	0.5360465421913201
1102	270-274	0.25418018757499006
1102	275-279	0.40028403222151177
1102	280-284	0.4524146539561116
1102	285-289	0.32715124785391225
1102	290-294	0.2851136208681506
1102	295-299	0.3367636195245911
1102	300-301	-0.12065997956693764
1103	1	0.004825341967979568
1103	2	0.1378132055579755
1103	3	0.0664067543557394
1103	4	0.1086595971404023
1103	5	0.09560284213355175
1103	6	0.21150249587819303
1103	7	1.0681817002456455
1103	8	0.3725732397406176
1103	9	0.3160119814697637
1103	10-14	0.19678301303680712
1103	15-19	0.262626891329802
1103	20-24	0.2264931988358896
1103	25-29	0.20607817351451274
1103	30-34	0.5929139259131588
1103	35-39	0.22942786414077432
1103	40-44	0.43923187525015805
1103	45-49	0.23517077661971797
1103	50-54	0.3182586508349061
1103	55-59	0.3686848002108718
1103	60-64	0.3182324097883651
1103	65-69	0.3940593031612494
1103	70-74	0.4114536453512727
1103	75-79	0.443052822472616
1103	80-84	0.9418659425080236
1103	85-89	0.5614656499235835
1103	90-94	0.5897394691010547
1103	95-99	1.2174181415976193
1103	100-104	0.749889825003919
1103	105-109	0.9335181696044685
1103	110-114	0.8268556095212958
1103	115-119	0.8688449777637643
1103	120-124	1.3448179261814062
1103	125-129	1.2433822296199466
1103	130-134	1.2825915406716035
1103	135-139	1.3094733486971322
1103	140-144	1.9344345088785388
1103	145-149	1.9268542109487186
1103	150-154	2.1838128424733334
1103	155-159	0.9989229611431298
1103	160-164	1.2701687934775947
1103	165-169	1.9998176358816444
1103	170-174	2.1254206369287694
1103	175-179	2.1601789096860955
1103	180-184	1.5595159850383382
1103	185-189	1.6866845534145156
1103	190-194	1.5387975935455316
1103	195-199	1.7954481268070346
1103	200-204	1.6513017359631021
1103	205-209	1.5850077524340698
1103	210-214	1.9442222084502028
1103	215-219	2.0136610146050167
1103	220-224	1.6419774121044846
1103	225-229	1.0389016131677664
1103	230-234	1.5383666776586367
1103	235-239	1.136042666072548
1103	240-244	0.6639191317604549
1103	245-249	1.015827273084323
1103	250-254	1.2349055598236003
1103	255-259	0.9461124469616689
1103	260-264	0.5812950811731898
1103	265-269	0.5776071216469827
1103	270-274	0.6240660523686685
1103	275-279	0.5513375879721707
1103	280-284	0.4268769015417142
1103	285-289	0.3206213971076419
1103	290-294	0.4052519000604242
1103	295-299	0.14174606026208103
1103	300-301	-0.07887442206035189
2116	1	0.13096068650175852
2116	2	0.09771746203000475
2116	3	0.06982986818782422
2116	4	0.1549961036010501
2116	5	0.15264767165321302
2116	6	0.16664392018881813
2116	7	1.0272558967680254
2116	8	0.28851573080000037
2116	9	0.25070503205395056
2116	10-14	0.1604064406739596
2116	15-19	0.21156540406968105
2116	20-24	0.21979193717617562
2116	25-29	0.1653698975733704
2116	30-34	0.429581372642474
2116	35-39	0.002323304857839048
2116	40-44	0.46093861043669193
2116	45-49	0.18067204016415417
2116	50-54	0.3400962166625092
2116	55-59	0.49689191783750886
2116	60-64	0.42119738078731217
2116	65-69	0.45664739396053733
2116	70-74	0.5609698232746538
2116	75-79	0.5255669067916315
2116	80-84	0.8575643629596996
2116	85-89	0.5345115537308729
2116	90-94	0.7399244067719479
2116	95-99	1.2866847204156286
2116	100-104	0.6323186259638689
2116	105-109	0.788456829980376
2116	110-114	0.9873424000242181
2116	115-119	0.9205606182292243
2116	120-124	1.1643826826369477
2116	125-129	1.1242471882111857
2116	130-134	1.1198715198140832
2116	135-139	1.4146088832730186
2116	140-144	2.041285448330491
2116	145-149	1.9749810397113592
2116	150-154	2.1705801005740817
2116	155-159	1.9680447310162705
2116	160-164	1.8483609119130868
2116	165-169	1.4376429259314456
2116	170-174	1.9318464160621467
2116	175-179	2.1919239517073876
2116	180-184	2.0490828303864674
2116	185-189	1.44217496414106
2116	190-194	1.6434434163100633
2116	195-199	0.8741159787501012
2116	200-204	1.0150445288910248
2116	205-209	0.9597184879284413
2116	210-214	2.1022408074472203
2116	215-219	2.1223478236409683
2116	220-224	1.4183327185984282
2116	225-229	0.7220725207052148
2116	230-234	1.2140755268672905
2116	235-239	0.7667072951527594
2116	240-244	0.6958402375856387
2116	245-249	1.0598568882411996
2116	250-254	0.9515114706043288
2116	255-259	1.5278269408921012
2116	260-264	0.788815565670621
2116	265-269	0.7426858634739943
2116	270-274	0.8045740145047429
2116	275-279	0.6018152847246494
2116	280-284	0.1850819302757678
2116	285-289	0.10987726446139234
2116	290-294	0.3389801335640623
2116	295-299	0.2861995792607921
2116	300-301	0.034558727879730355
1117	1	0.11045908356865652
1117	2	0.1476217368939885
1117	3	0.17360992418984722
1117	4	0.05051884013230534
1117	5	0.13133775890881338
1117	6	0.10450953143688224
1117	7	1.0630751316432168
1117	8	0.5049149199139009
1117	9	0.3258971666520196
1117	10-14	0.29012953820733145
1117	15-19	0.3443109684293404
1117	20-24	0.2887290097743147
1117	25-29	0.3155148941839414
1117	30-34	0.5682445055031664
1117	35-39	0.3413955841787981
1117	40-44	0.6678164896378505
1117	45-49	0.3464463993531979
1117	50-54	0.2511892222063494
1117	55-59	0.549645565295279
1117	60-64	0.5455529469342721
1117	65-69	0.632671346981958
1117	70-74	0.5389172960183402
1117	75-79	0.5592853412468912
1117	80-84	1.074420198811623
1117	85-89	0.8274306309695447
1117	90-94	0.6792765351869292
1117	95-99	1.2174661885445488
1117	100-104	0.9153328486741543
1117	105-109	0.7657070952573548
1117	110-114	0.9478567877778445
1117	115-119	0.9646962661410683
1117	120-124	1.2587373953890832
1117	125-129	1.1473210519481896
1117	130-134	1.2411022117562638
1117	135-139	1.2224544799972747
1117	140-144	1.4212144072371231
1117	145-149	1.8469331723128093
1117	150-154	2.3276541800750543
1117	155-159	1.856862521560977
1117	160-164	2.0049648191732246
1117	165-169	1.5854026035092232
1117	170-174	1.3903279529457073
1117	175-179	1.7503270728781821
1117	180-184	1.6669096041193008
1117	185-189	1.218983718361745
1117	190-194	-0.5005135017431037
1117	195-199	1.1680861807792446
1117	200-204	1.5972536621478213
1117	205-209	1.6777489492930044
1117	210-214	2.0478103196757225
1117	215-219	1.709444815987787
1117	220-224	1.7189833918947386
1117	225-229	1.214341246683361
1117	230-234	1.6310669397831816
1117	235-239	0.9706457927592282
1117	240-244	1.084666562078553
1117	245-249	1.1283086529167718
1117	250-254	0.7031508742584673
1117	255-259	0.7865646572397651
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2108	255-259	-0.6457591822870423
2108	260-264	-0.6171169030234758
2108	265-269	-0.30152541942914723
2108	270-274	-0.40220307468513994
2108	275-279	-0.36379645818360373
2108	280-284	-0.7266098662997518
2108	285-289	-1.049405255344384
2108	290-294	-0.8454940550380332
2108	295-299	-0.6354698559551544
2108	300-301	2.5690655001771994
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	44.0
11	348.0
12	733.0
13	846.0
14	1287.0
15	2025.0
16	2870.0
17	4131.0
18	5313.0
19	6153.0
20	7892.0
21	10098.0
22	13306.0
23	17523.0
24	23389.0
25	31545.0
26	42302.0
27	53099.0
28	62055.0
29	61246.0
30	45510.0
31	22599.0
32	6378.0
33	792.0
34	31.0
35	4.0
36	0.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.185440518497074	19.237247524470614	14.28761215203807	34.289699804994235
2	49.11583152640644	22.757552321802212	14.546908138525264	13.579708013266087
3	48.79224531820101	21.685969548734835	16.862542286834024	12.659242846230127
4	49.00931377898397	20.31618452954266	17.20914012421535	13.465361567258011
5	31.211835094395123	20.570261383639444	35.34230391482376	12.875599607141671
6	29.981543250001184	20.236948610524617	35.561507475220985	14.220000664253213
7	15.960344083164502	51.323761760840384	18.643689831706702	14.072204324288418
8	29.122521505197778	18.90773048400336	36.98206041857442	14.987687592224441
9	14.496851914235421	17.56665069295844	37.95851264216205	29.977984750644087
10-14	27.074533955200863	35.63974701441904	22.221499978649003	15.064219051731092
15-19	21.596959618149295	35.888557289467315	20.498901609865108	22.01558148251828
20-24	25.25481227729725	30.586820267314472	26.25593676309409	17.90243069229419
25-29	19.140693575248026	24.867173080664063	38.92927126677833	17.062862077309585
30-34	19.615207602852493	28.018675004626047	31.159121857251986	21.206995535269474
35-39	20.20534913623359	29.91521282198488	27.87192249113934	22.00751555064219
40-44	20.75103315098001	28.29301158172924	29.551534187689487	21.404421079601256
45-49	21.432367161219	29.940596784065516	26.173569364641804	22.453466690073682
50-54	21.920071359773775	29.346232498113995	27.43465409013916	21.299042051973068
55-59	21.81250978587323	26.45421634727158	28.880590995573225	22.852682871281964
60-64	21.781859244744094	26.24189255229808	28.669405920394	23.306842282563828
65-69	19.50356561635581	27.338100140916577	29.128167657511046	24.03016658521657
70-74	20.79131536370236	25.48649430877336	29.262109573312205	24.460080754212076
75-79	20.460612156782737	25.1862518563505	29.881098674814837	24.472037312051924
80-84	24.696839578104317	24.304313375687382	26.95881155610805	24.040035490100255
85-89	20.733525334143092	28.445315354213026	26.040149362079685	24.781009949564204
90-94	20.5139896471392	29.881762928028166	28.65479234970085	20.949455075131784
95-99	21.347485089887694	29.88062420823389	26.383520826710573	22.388369875167843
100-104	21.965715044860815	28.77544920123551	25.746596888448163	23.51223886545551
105-109	24.07301091747603	26.851819342104637	23.590478404653567	25.484691335765763
110-114	20.294501406793415	28.17116856374221	29.294847767397503	22.239482262066872
115-119	23.877151112861366	27.387112538728335	25.822131968134826	22.913604380275476
120-124	18.523839573359652	28.011415665937573	28.19655252582284	25.268192234879937
125-129	20.64418327695089	24.229015529291196	31.106123940160273	24.02067725359764
130-134	25.267385641692325	28.08434117942903	25.24959314490684	21.398680033971807
135-139	24.19827009484587	27.25611231572904	24.12757457428486	24.418043015140228
140-144	20.90286245688285	27.584158509795365	26.88773646228228	24.62524257103951
145-149	20.02248971593686	27.2201951955514	29.073746340676493	23.683568747835245
150-154	22.930258157266692	25.006761148778484	25.8798271043779	26.183153589576918
155-159	22.227003790987983	25.937996707201926	29.726090442819658	22.10890905899043
160-164	24.913243785673956	28.24528024368604	25.831621299753753	21.009854670886256
165-169	20.33193682003008	25.019334513173565	28.292679455122578	26.356049211673778
170-174	22.343580229926506	28.494280305366694	26.32966886977316	22.832470594933646
175-179	20.761566309077022	28.646157057927624	27.562190707097546	23.030085925897808
180-184	19.912366022499207	29.25646342099894	26.927544208423683	23.903626348078173
185-189	22.006661510796487	26.7716344899247	27.798380171092653	23.423323828186163
190-194	19.97679858419172	26.906382998913468	27.30521960685699	25.811598810037818
195-199	20.39821980138829	29.42712905016535	27.85863742687284	22.316013721573523
200-204	22.20304322865019	27.158134966763615	26.845746169868523	23.79307563471767
205-209	21.081499124609156	26.98993656381813	28.480568221177343	23.447996090395375
210-214	23.101066126407385	26.656623790703303	27.51896680157333	22.72334328131598
215-219	22.94515640790841	26.36743640961649	28.415423959613406	22.271983222861696
220-224	18.597049766799675	26.947234571532952	29.04589515237494	25.40982050929243
225-229	21.685874655418647	27.973600679436146	26.603436086979215	23.737088578165995
230-234	21.051275603402875	26.440883836346984	29.009218885667785	23.498621674582353
235-239	21.512931586663694	26.67503309404402	25.961103229694015	25.850932089598267
240-244	22.383435422726	30.731105554580264	23.780786950271157	23.10467207242258
245-249	20.635215858571	28.893876059839723	24.566788288266917	25.904119793322355
250-254	21.37936924412729	26.984480198137245	25.477574337051568	26.158576220683894
255-259	21.295056532693117	27.219673282312364	27.453300626770353	24.031969558224166
260-264	21.19119579812396	28.405175481464962	26.586687416671808	23.816941303739274
265-269	20.484809952411002	28.254390002040207	26.97266598027168	24.288134065277113
270-274	19.632430739740848	29.990273435090597	25.337369462381915	25.039926362786634
275-279	20.401066600873968	29.188235126658853	23.908987820442867	26.501710452024312
280-284	18.10350962929926	29.822929071990817	27.310960652486443	24.762600646223483
285-289	20.32344386823114	28.611663337492825	27.342892253384132	23.7220005408919
290-294	19.303150932564066	30.930666198526307	25.773926163510673	23.992256705398958
295-299	18.914562802768987	32.33589387131517	23.275527488221368	25.474015837694473
300-301	14.271005821704948	33.627225841347865	30.37226647941052	21.729501857536665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.25
26	0.5
27	0.5
28	0.5
29	1.75
30	3.5
31	4.75
32	13.5
33	35.25
34	89.0
35	228.75
36	644.5
37	1684.25
38	3462.75
39	5501.5
40	7728.25
41	10643.5
42	14148.25
43	16328.5
44	16067.0
45	13335.0
46	10002.25
47	7870.5
48	6581.75
49	5624.75
50	4811.0
51	3605.75
52	2228.0
53	1177.5
54	752.25
55	614.5
56	600.0
57	644.0
58	642.75
59	699.25
60	794.25
61	885.75
62	877.5
63	734.5
64	520.5
65	315.0
66	176.0
67	92.0
68	46.75
69	27.5
70	15.0
71	11.25
72	9.5
73	8.75
74	6.25
75	3.25
76	2.5
77	3.25
78	1.75
79	2.25
80	2.0
81	1.5
82	2.0
83	1.5
84	1.0
85	1.0
86	1.5
87	1.0
88	0.5
89	1.25
90	0.5
91	0.0
92	0.25
93	2.0
94	0.75
95	0.0
96	0.25
97	0.25
98	0.25
99	1.25
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	421526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.592278859602963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.484551812498076	8.534228890268698
2	4.978756464982485	1.45302805422151
3	3.1373895319524676	1.373449888243489
4	2.848387792896279	1.6625787589692607
5	2.983968009477273	2.1771446651213378
6	2.770635063822401	2.42579276816942
7	2.719363968596468	2.777720214536138
8	2.56503670711482	2.994378473226975
9	2.198421087218083	2.8871976199966016
>10	16.881688120561126	42.171398310855864
>50	0.32550090225699446	2.946748755208037
>100	0.05934075112192108	1.567369150315294
>500	0.010062811614417435	0.9338982862095717
>1k	0.02683416428161007	11.181572003311063
>5k	0.006708541070402517	7.8906573689674815
>10k+	0.0033542705352012587	7.022836792379221
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	18600	4.412539202801251	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	10096	2.3951073006172807	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9558	2.2674757903427074	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	8449	2.0043840712079444	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	8292	1.9671384446036544	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5943	1.4098774452821417	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4807	1.1403804273046028	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4674	1.108828399671669	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4660	1.1055071336050446	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	4438	1.0528413431199974	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4355	1.033150980010723	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	3250	0.7710081940378529	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	3232	0.7667379948093356	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	3115	0.7389816998239729	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2620	0.6215512210397461	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2101	0.4984271432841628	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1987	0.4713825481702196	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1572	0.3729307326238476	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	1427	0.33853190550523576	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1213	0.2877639813439741	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1142	0.2709204177203779	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1096	0.2600076863586113	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	711	0.1686728695264349	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	650	0.1542016388075706	No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG	649	0.15396440551709742	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	636	0.15088037274094598	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	609	0.14447507389817	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	561	0.13308787595545707	No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG	450	0.10675498071293349	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC	442	0.10485711438914799	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.372332904731855E-4	0.0	0.0
2	2.372332904731855E-4	0.0	0.0
3	2.372332904731855E-4	0.0	0.0
4	4.74466580946371E-4	0.0	0.0
5	4.74466580946371E-4	0.0	0.0
6	4.74466580946371E-4	0.0	0.0
7	4.74466580946371E-4	0.0	0.0
8	9.48933161892742E-4	0.0	0.0
9	9.48933161892742E-4	0.0	0.0
10-14	0.0022299929304479436	0.0	0.0
15-19	0.0026570128532996777	0.0	0.0
20-24	0.0031314794342460492	0.0	0.0
25-29	0.0034161593828138715	0.0	0.0
30-34	0.006073172236113549	0.0	0.0
35-39	0.006642532133249194	0.0	0.0
40-44	0.007259338688479477	0.0	0.0
45-49	0.01456612403505359	0.0	0.0
50-54	0.02514672879015766	0.0	0.0
55-59	0.028610334831066175	0.0	0.0
60-64	0.03411414717004408	0.0	0.0
65-69	0.04331879884040367	0.0	0.0
70-74	0.058644069404971466	0.0	0.0
75-79	0.07790741259139414	0.0	0.0
80-84	0.10390818122725527	0.0	0.0
85-89	0.13688360860302803	0.0	0.0
90-94	0.17745050127394277	0.0	0.0
95-99	0.23163458481801835	0.0	0.0
100-104	0.30005266579048506	0.0	0.0
105-109	0.3799528380218539	0.0	0.0
110-114	0.47408700768161394	0.0	0.0
115-119	0.5807470950783582	0.0	0.0
120-124	0.6923890815750393	0.0	0.0
125-129	0.8226776047029126	0.0	0.0
130-134	0.9656343855420543	0.0	0.0
135-139	1.1267157897733473	0.0	0.0
140-144	1.291877606600779	0.0	0.0
145-149	1.4946171766391634	0.0	0.0
150-154	1.7159558366506455	0.0	0.0
155-159	1.967043551287465	0.0	0.0
160-164	2.2383435422725997	0.0	0.0
165-169	2.5578018912237916	0.0	0.0
170-174	2.899892296086125	0.0	0.0
175-179	3.2893819123850014	0.0	0.0
180-184	3.689926600019928	0.0	0.0
185-189	4.146648130838905	0.0	0.0
190-194	4.646119100601149	0.0	0.0
195-199	5.143692204039608	0.0	0.0
200-204	5.617589425088844	0.0	0.0
205-209	6.092150899351404	0.0	0.0
210-214	6.585643590193724	0.0	0.0
215-219	7.090713265611138	0.0	0.0
220-224	7.604323339485584	0.0	0.0
225-229	8.095965610662214	0.0	0.0
230-234	8.541299943538478	0.0	0.0
235-239	8.941132931301983	0.0	0.0
240-244	9.310552611226829	0.0	0.0
245-249	9.593334693470865	0.0	0.0
250-254	9.86136086504747	0.0	0.0
255-259	10.077005926087596	0.0	0.0
260-264	10.274146790470812	0.0	0.0
265-269	10.425786309741273	0.0	0.0
270-274	10.545541674772137	0.0	4.74466580946371E-5
275-279	10.628620773095847	0.0	2.3723329047318549E-4
280-284	10.68849845561128	0.0	2.3723329047318549E-4
285-289	10.731342787870739	0.0	2.3723329047318549E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7355	0.0	288.78314	1
GGGTTAT	7385	0.0	287.21057	2
GTTATTA	7385	0.0	286.2119	4
GGTTATT	7460	0.0	283.53217	3
GGGGGGA	6595	0.0	278.67325	1
TTATTAG	7585	0.0	276.33157	5
TTAGTAG	7710	0.0	275.10376	8
GGGAGCG	5050	0.0	269.8812	4
GGGGAGC	6850	0.0	269.80658	3
GGAGCGA	5050	0.0	269.29703	5
ATTAGTA	7840	0.0	267.90817	7
TATTAGT	7915	0.0	266.11496	6
TAGTAGA	8025	0.0	264.67288	9
CGAAAGC	5125	0.0	264.49268	9
GAGCGAA	5315	0.0	254.76012	6
GGGGGAG	7325	0.0	252.51195	2
GCGAAAG	5450	0.0	250.34404	8
AGCGAAA	5740	0.0	235.38327	7
GGGAGCA	2085	0.0	232.7458	4
CAAAAGC	2870	0.0	160.34843	9
>>END_MODULE