FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_30112_flu.3520000003bb25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_30112_flu.3520000003bb25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421526
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC186004.412539202801251No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG100962.3951073006172807No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT95582.2674757903427074No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA84492.0043840712079444No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG82921.9671384446036544No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA59431.4098774452821417No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT48071.1403804273046028No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT46741.108828399671669No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA46601.1055071336050446No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA44381.0528413431199974No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT43551.033150980010723No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC32500.7710081940378529No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG32320.7667379948093356No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA31150.7389816998239729No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT26200.6215512210397461No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG21010.4984271432841628No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG19870.4713825481702196No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC15720.3729307326238476No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT14270.33853190550523576Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12130.2877639813439741No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11420.2709204177203779No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10960.2600076863586113No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT7110.1686728695264349No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6500.1542016388075706No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG6490.15396440551709742No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6360.15088037274094598No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6090.14447507389817No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT5610.13308787595545707No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG4500.10675498071293349No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC4420.10485711438914799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA73550.0288.783141
GGGTTAT73850.0287.210572
GTTATTA73850.0286.21194
GGTTATT74600.0283.532173
GGGGGGA65950.0278.673251
TTATTAG75850.0276.331575
TTAGTAG77100.0275.103768
GGGAGCG50500.0269.88124
GGGGAGC68500.0269.806583
GGAGCGA50500.0269.297035
ATTAGTA78400.0267.908177
TATTAGT79150.0266.114966
TAGTAGA80250.0264.672889
CGAAAGC51250.0264.492689
GAGCGAA53150.0254.760126
GGGGGAG73250.0252.511952
GCGAAAG54500.0250.344048
AGCGAAA57400.0235.383277
GGGAGCA20850.0232.74584
CAAAAGC28700.0160.348439
Produced by FastQC (version 0.11.2)