##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_30082_flu.3520000003b984.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297291
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31312754170157	34.0	34.0	34.0	34.0	34.0
2	33.51019707962905	34.0	34.0	34.0	34.0	34.0
3	33.517048952036895	34.0	34.0	34.0	34.0	34.0
4	33.525905594182134	34.0	34.0	34.0	34.0	34.0
5	33.47343175541809	34.0	34.0	34.0	34.0	34.0
6	37.00280533214931	38.0	38.0	38.0	36.0	38.0
7	36.09193349277307	38.0	38.0	38.0	34.0	38.0
8	36.8912412417463	38.0	38.0	38.0	35.0	38.0
9	36.960123246246944	38.0	38.0	38.0	36.0	38.0
10-14	36.99734267098567	38.0	38.0	38.0	36.2	38.0
15-19	36.91185807844839	38.0	38.0	38.0	36.2	38.0
20-24	37.00652962921851	38.0	38.0	38.0	36.2	38.0
25-29	36.920363549518825	38.0	38.0	38.0	36.0	38.0
30-34	36.57759703455537	38.0	37.8	38.0	34.6	38.0
35-39	36.86244925006139	38.0	38.0	38.0	35.8	38.0
40-44	36.392215035100286	38.0	37.2	38.0	33.8	38.0
45-49	36.69346061602941	38.0	38.0	38.0	35.0	38.0
50-54	36.65963449953076	38.0	38.0	38.0	34.6	38.0
55-59	36.31196773531658	38.0	37.0	38.0	33.6	38.0
60-64	36.489120087725496	38.0	37.2	38.0	34.0	38.0
65-69	36.36534035675483	38.0	37.0	38.0	34.0	38.0
70-74	36.23152264952522	38.0	37.0	38.0	33.6	38.0
75-79	36.274301610206834	38.0	37.0	38.0	34.0	38.0
80-84	35.62944724192795	38.0	36.8	38.0	30.2	38.0
85-89	35.851366506217815	38.0	37.0	38.0	31.8	38.0
90-94	35.82986568715501	38.0	37.0	38.0	31.6	38.0
95-99	34.92469533218294	38.0	35.8	38.0	27.0	38.0
100-104	35.250496651429074	38.0	36.0	38.0	28.2	38.0
105-109	35.072035816758664	38.0	35.8	38.0	27.2	38.0
110-114	34.60188502174637	38.0	35.0	38.0	24.0	38.0
115-119	34.64601081095627	38.0	35.0	38.0	24.4	38.0
120-124	34.179497529356766	37.6	34.6	38.0	23.6	38.0
125-129	33.725306854227	37.0	34.0	38.0	23.0	38.0
130-134	33.518385689442326	37.0	33.2	38.0	22.8	38.0
135-139	32.899102899179596	37.0	30.8	38.0	21.2	38.0
140-144	31.620500452418675	36.0	27.6	38.0	17.2	38.0
145-149	31.46964085693815	35.0	28.0	37.6	17.8	38.0
150-154	30.430015708514553	35.0	25.4	37.0	13.4	38.0
155-159	30.277162107160997	35.0	25.2	37.0	13.2	37.4
160-164	29.53427651694804	34.4	24.0	37.0	10.2	37.4
165-169	28.450516833674747	32.8	22.4	36.6	9.8	37.0
170-174	27.76313914649283	32.2	21.0	36.2	9.2	37.0
175-179	26.941509833799206	29.8	20.0	35.6	9.2	37.0
180-184	26.0268430594939	28.2	19.6	35.0	9.0	37.0
185-189	25.257063281431325	26.8	17.8	35.0	8.6	36.6
190-194	24.217405841414642	25.6	15.8	34.6	8.2	36.0
195-199	23.377294973611715	24.4	15.0	33.6	8.0	36.0
200-204	22.51587299985536	23.4	13.6	32.2	8.0	36.0
205-209	21.65068367357236	22.6	10.0	31.0	8.0	35.2
210-214	20.318237013565835	20.4	9.0	28.2	8.0	35.0
215-219	19.780056577562053	19.6	9.0	27.8	8.0	34.0
220-224	19.075668620980785	18.6	9.0	26.2	8.0	33.6
225-229	18.29527903636504	16.8	9.0	25.6	8.0	32.4
230-234	17.40574050341248	15.6	9.0	24.6	8.0	31.4
235-239	16.446750826631146	14.6	8.4	22.8	7.2	29.8
240-244	15.608839823607173	13.4	8.0	21.8	7.0	28.8
245-249	14.953342011698973	12.8	8.0	20.4	7.0	27.4
250-254	14.262662509124056	12.0	8.0	19.2	7.0	26.0
255-259	13.679041074233663	11.2	8.0	18.2	7.0	24.8
260-264	13.066417079561777	10.2	8.0	16.6	7.0	23.6
265-269	12.680401357592391	9.0	8.0	16.0	7.0	22.6
270-274	12.22411038342903	9.0	7.4	14.6	7.0	21.6
275-279	11.7290190419488	8.4	7.0	14.0	7.0	20.8
280-284	11.60233441308348	8.0	7.0	13.8	7.0	20.6
285-289	11.298972387324202	8.0	7.0	13.0	7.0	19.0
290-294	11.030621175884908	8.0	7.0	13.0	7.0	19.0
295-299	10.737101358601505	8.0	7.0	13.0	7.0	18.6
300-301	10.437776454719451	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.20966178856022566
1101	2	0.11387376411319394
1101	3	0.25888353642105955
1101	4	0.18808536594127645
1101	5	0.18948405125710366
1101	6	0.37293978222184876
1101	7	1.1557673283938428
1101	8	0.36933252806429806
1101	9	0.1254170267514425
1101	10-14	0.2711280802140337
1101	15-19	0.2855541623236988
1101	20-24	0.24729712739210186
1101	25-29	0.21771914529410452
1101	30-34	0.700577892927889
1101	35-39	0.38361952348414263
1101	40-44	0.5454091664355545
1101	45-49	0.2543218734620183
1101	50-54	0.3317428059845753
1101	55-59	0.4566542223277068
1101	60-64	0.357746570065558
1101	65-69	0.397426357843095
1101	70-74	0.336873699801437
1101	75-79	0.39940820150075496
1101	80-84	1.040745099405548
1101	85-89	0.5244752424605537
1101	90-94	0.5877796298790301
1101	95-99	0.9592622804513979
1101	100-104	0.40118527931891634
1101	105-109	0.72103578275388
1101	110-114	0.8466977356867318
1101	115-119	0.6148499046790477
1101	120-124	0.9111738256238624
1101	125-129	0.8319561878767985
1101	130-134	0.9700094462490796
1101	135-139	0.452141267982789
1101	140-144	1.2126790081646561
1101	145-149	1.363912762696657
1101	150-154	1.0294929258096452
1101	155-159	0.7035754620648262
1101	160-164	1.2444621449369393
1101	165-169	0.7866988836793283
1101	170-174	0.7801873246127329
1101	175-179	0.503682731768393
1101	180-184	0.7900373789387771
1101	185-189	0.466785548857235
1101	190-194	0.6201471234639229
1101	195-199	0.6835997791490414
1101	200-204	0.6258631219158843
1101	205-209	0.3131781564410403
1101	210-214	0.8270170407249786
1101	215-219	0.8646112903292149
1101	220-224	0.1842787340238523
1101	225-229	0.25388815088170347
1101	230-234	0.22241477290370426
1101	235-239	0.20241038757066576
1101	240-244	0.06722398031265264
1101	245-249	-0.30303514861168424
1101	250-254	-0.044492590989190006
1101	255-259	-0.006220132040205328
1101	260-264	0.20047344329988803
1101	265-269	0.14987629858019957
1101	270-274	0.05282206627315844
1101	275-279	-0.05296901285756839
1101	280-284	0.033814453858401095
1101	285-289	-0.09066705100381611
1101	290-294	-0.03563675428716628
1101	295-299	0.06696858611467427
1101	300-301	-0.324536170066585
1102	1	-0.1132437372864743
1102	2	-0.043865392155375105
1102	3	0.10855673903543561
1102	4	0.043596358216973385
1102	5	-0.04129432069773742
1102	6	-0.26854863132538753
1102	7	0.7687798061121995
1102	8	-0.05997525565525308
1102	9	-0.018403531775000204
1102	10-14	-0.048877861556618996
1102	15-19	0.033085356619601214
1102	20-24	-0.04072129209690445
1102	25-29	0.01505723204396503
1102	30-34	0.32925584895878757
1102	35-39	0.012318275712310367
1102	40-44	0.2958102359542707
1102	45-49	0.05828800536933443
1102	50-54	0.08904821299586274
1102	55-59	0.18065600485890343
1102	60-64	0.08826944588254548
1102	65-69	0.06497537745094917
1102	70-74	0.16582794816743984
1102	75-79	0.2648568051846567
1102	80-84	0.5875573394530846
1102	85-89	0.059998118277547974
1102	90-94	0.18677547063957434
1102	95-99	0.7713656672606604
1102	100-104	0.5860872401032324
1102	105-109	0.7616210471626843
1102	110-114	0.5845586982535735
1102	115-119	0.5087417644532692
1102	120-124	0.8558826788621232
1102	125-129	0.7752271326183404
1102	130-134	1.1068929875443843
1102	135-139	1.0026522602584933
1102	140-144	2.070801408639994
1102	145-149	1.7448812713122237
1102	150-154	1.8245434308601496
1102	155-159	1.1202688666994085
1102	160-164	1.3254960130296318
1102	165-169	1.2509353661511078
1102	170-174	1.4909330168290111
1102	175-179	1.1678538547630417
1102	180-184	1.681784259509186
1102	185-189	1.4470172779124937
1102	190-194	1.6385161074211432
1102	195-199	1.9819271707117245
1102	200-204	1.9556611017138614
1102	205-209	1.6974294933394347
1102	210-214	2.105662317017316
1102	215-219	1.863206061571045
1102	220-224	1.571126624695271
1102	225-229	1.2258043719155722
1102	230-234	1.4560036023748886
1102	235-239	1.1372351053365648
1102	240-244	1.190456303544977
1102	245-249	0.6480700207287811
1102	250-254	0.9963481695574501
1102	255-259	0.9248404740203977
1102	260-264	0.6386166399724971
1102	265-269	0.643070000303311
1102	270-274	0.6113900995470729
1102	275-279	0.5982520210105271
1102	280-284	0.4006653154151447
1102	285-289	0.3815551712184053
1102	290-294	0.296517137572609
1102	295-299	0.112919269418299
1102	300-301	-0.04447823780277105
1103	1	-0.213536451509178
1103	2	0.02318621376306851
1103	3	0.0737437954530975
1103	4	0.10516257979762855
1103	5	-0.032440717906929706
1103	6	0.02601811152717204
1103	7	0.9399093622291304
1103	8	0.3673634507884671
1103	9	0.11230740290562125
1103	10-14	0.10209852081636228
1103	15-19	0.09004353457380887
1103	20-24	0.06455157369562414
1103	25-29	-0.05209060868742199
1103	30-34	0.39274837071186397
1103	35-39	0.11391015990975717
1103	40-44	0.4506440304127466
1103	45-49	0.14391132627233105
1103	50-54	0.11711028445020588
1103	55-59	0.3657107384087652
1103	60-64	0.18746027149704503
1103	65-69	0.3874502160571396
1103	70-74	0.21393573115795306
1103	75-79	0.2113342100525344
1103	80-84	0.7954117041028255
1103	85-89	0.36382425404883634
1103	90-94	0.22080249658286988
1103	95-99	0.9350514605964051
1103	100-104	0.6201014347381388
1103	105-109	0.779418071903045
1103	110-114	0.5145430600964715
1103	115-119	0.7139352365251241
1103	120-124	0.862205306072525
1103	125-129	0.6924764208817038
1103	130-134	0.871374322000463
1103	135-139	0.8254727422345098
1103	140-144	1.2925761389820352
1103	145-149	1.3492824100908436
1103	150-154	1.7776995317463111
1103	155-159	0.8371941644258563
1103	160-164	0.6342285203474844
1103	165-169	1.6031932754497937
1103	170-174	2.0388298851878055
1103	175-179	1.864805617063059
1103	180-184	1.2109650606640052
1103	185-189	1.1142757886787642
1103	190-194	1.3084996673072666
1103	195-199	1.6520855571746935
1103	200-204	1.7356747349790282
1103	205-209	1.2400719409115126
1103	210-214	1.4991023106016605
1103	215-219	1.4831218561097224
1103	220-224	1.3849700025372051
1103	225-229	0.8958119905049315
1103	230-234	1.802464968107273
1103	235-239	1.176832523035749
1103	240-244	0.8916151310841762
1103	245-249	0.8175331106682311
1103	250-254	0.8785070371944883
1103	255-259	0.8522297037409157
1103	260-264	0.8338417893369439
1103	265-269	0.9118875150392682
1103	270-274	0.38671870133543607
1103	275-279	0.243362089577829
1103	280-284	0.6478684911639725
1103	285-289	0.300409976944378
1103	290-294	0.059684866832000694
1103	295-299	0.0876319684038247
1103	300-301	-0.19979721610984136
2116	1	-0.08606676703269756
2116	2	0.01955667210805956
2116	3	0.08899725062848773
2116	4	0.07008507947612941
2116	5	0.16940658111798257
2116	6	0.02741455460660802
2116	7	0.9536786238390462
2116	8	0.28100940783611605
2116	9	0.17672231141060735
2116	10-14	0.03743791095048721
2116	15-19	0.15507209130525723
2116	20-24	0.1922917717393986
2116	25-29	0.0906830631408937
2116	30-34	0.396973102732467
2116	35-39	-0.137060768959401
2116	40-44	0.3784420227421421
2116	45-49	0.16118364780221128
2116	50-54	0.1518261922516828
2116	55-59	0.397990208577383
2116	60-64	0.33072232016830583
2116	65-69	0.435174679530995
2116	70-74	0.3553214323675391
2116	75-79	0.4883201321512942
2116	80-84	0.7152431626520723
2116	85-89	0.5454541436798976
2116	90-94	0.45271069141920606
2116	95-99	1.188603161518543
2116	100-104	0.5827923487804867
2116	105-109	0.6897298130693699
2116	110-114	0.9098931921004336
2116	115-119	0.6532424615672952
2116	120-124	0.985506237286728
2116	125-129	1.2699959567118881
2116	130-134	1.0535367663053776
2116	135-139	1.440145228674794
2116	140-144	1.7306764922533944
2116	145-149	1.8970735941928822
2116	150-154	2.240331895033325
2116	155-159	1.8016875944864559
2116	160-164	1.987137978488235
2116	165-169	1.7248329991870577
2116	170-174	1.8020218847641836
2116	175-179	2.1307125864184684
2116	180-184	2.1573598016866065
2116	185-189	1.3830449368182265
2116	190-194	1.290266629249274
2116	195-199	0.8895828527954919
2116	200-204	1.070608354762225
2116	205-209	0.8887504639800561
2116	210-214	1.8085985774236555
2116	215-219	1.9944475692715358
2116	220-224	1.0302286773535734
2116	225-229	1.0537053736644033
2116	230-234	1.1814496344663077
2116	235-239	1.0402019904054285
2116	240-244	0.5104179421256063
2116	245-249	1.0349694348306304
2116	250-254	0.9285438772692274
2116	255-259	1.497768272348937
2116	260-264	0.41991851049464834
2116	265-269	0.9441691033230661
2116	270-274	0.4826075910648484
2116	275-279	0.7598593322457479
2116	280-284	0.3437392256204106
2116	285-289	0.12820539727677094
2116	290-294	0.4275839235142769
2116	295-299	0.2502405785420301
2116	300-301	-0.1838841193498002
1117	1	0.17773471109411076
1117	2	0.11626289707853488
1117	3	0.1675989432777314
1117	4	0.1486623251241781
1117	5	0.16383724865863059
1117	6	0.20726350269798388
1117	7	1.089021806257989
1117	8	0.4056952256723463
1117	9	0.3409083714249377
1117	10-14	0.2001412603424484
1117	15-19	0.16374405380355483
1117	20-24	0.24670101759524954
1117	25-29	0.3025300799191726
1117	30-34	0.5102855644385329
1117	35-39	0.2885386027526735
1117	40-44	0.6339009617117313
1117	45-49	0.2509939849110836
1117	50-54	0.2751142527946726
1117	55-59	0.3089044194663799
1117	60-64	0.3980811425562649
1117	65-69	0.3963834003874993
1117	70-74	0.4817518493264217
1117	75-79	0.4830456916438308
1117	80-84	0.9148251189316596
1117	85-89	0.597038232960351
1117	90-94	0.6259513095005147
1117	95-99	1.0867997367992643
1117	100-104	0.6672943251677381
1117	105-109	0.7109244463414299
1117	110-114	0.8479481581267407
1117	115-119	0.8851326578614334
1117	120-124	1.0391496808302065
1117	125-129	1.0036742983495657
1117	130-134	1.0137132125364943
1117	135-139	1.163265521672102
1117	140-144	1.3337146433266938
1117	145-149	1.8768131419539102
1117	150-154	2.26318861879901
1117	155-159	1.6032515913877923
1117	160-164	2.131402177230136
1117	165-169	1.3792770820196054
1117	170-174	1.4139196290699445
1117	175-179	1.6245343708356472
1117	180-184	1.6489480469572513
1117	185-189	1.4401042376034354
1117	190-194	-0.5906006892355613
1117	195-199	1.5066340249528025
1117	200-204	1.6343889001442626
1117	205-209	1.6454704098299473
1117	210-214	1.6760576325165033
1117	215-219	1.5648694481152567
1117	220-224	1.5267414654271043
1117	225-229	1.5484799799530897
1117	230-234	1.2995072591039971
1117	235-239	0.8975082059026889
1117	240-244	0.9497293312194941
1117	245-249	1.3636071104097578
1117	250-254	0.9081969437635422
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2108	250-254	-0.738357294360938
2108	255-259	-0.592814953494182
2108	260-264	-0.7743273818530501
2108	265-269	-0.7228972111651419
2108	270-274	-0.5830507463602181
2108	275-279	-0.4952118639032346
2108	280-284	-0.8267987201350557
2108	285-289	-1.2935621996736124
2108	290-294	-0.8400434977784155
2108	295-299	-0.7084724439038208
2108	300-301	2.5731822109264417
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	46.0
12	208.0
13	374.0
14	533.0
15	1075.0
16	1613.0
17	2234.0
18	2538.0
19	3202.0
20	4370.0
21	5911.0
22	8107.0
23	11591.0
24	16209.0
25	22554.0
26	31293.0
27	40789.0
28	47346.0
29	45578.0
30	32654.0
31	14854.0
32	3777.0
33	413.0
34	14.0
35	2.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.91375790050826	23.086134460848125	14.730012008436177	33.27009563020744
2	44.12747106370526	24.422871866285895	17.27734778382124	14.172309286187607
3	43.27712577911878	23.534516685671612	18.832053442586556	14.356304092623054
4	43.527049254770574	22.53213181697394	18.981065689845973	14.959753238409505
5	27.703832272083584	22.775327877399583	35.142335287647455	14.378504562869377
6	26.731384401142318	21.95660144437605	35.702392605225185	15.609621549256453
7	17.051306632222303	47.18272668866532	20.52164377663636	15.244322902476027
8	26.0872343932376	20.908806522901806	36.75960590801605	16.24435317584454
9	15.647967816045558	19.670289379765954	37.97995903004127	26.701783774147213
10-14	26.133788106602623	34.04543023502225	23.859787211856396	15.96099444651873
15-19	21.17756676118685	35.341466778341754	22.08738239637258	21.39358406409881
20-24	25.136650621781353	30.958488484346987	26.50763057072027	17.39723032315139
25-29	19.259984325122524	25.79109357498209	37.620513234507605	17.328408865387786
30-34	19.45407025439721	28.49410173870047	31.19327527573993	20.858552731162398
35-39	20.78630029163345	29.980725955377054	27.97635986289528	21.25661389009422
40-44	20.23196127699796	29.136906263559947	28.85011655246879	21.781015906973302
45-49	20.772576364572085	30.552758072057344	26.563064472183818	22.11160109118675
50-54	21.939513809701605	29.598877867140278	27.932698938077504	20.528909385080613
55-59	21.104237935221718	27.357774032849967	29.97117302575591	21.566815006172405
60-64	22.035446750826633	26.321752088021498	28.304994096693136	23.33780706445873
65-69	20.17942016408166	27.264868428576715	29.206333188693907	23.349378218647722
70-74	20.977157061599577	26.395148188138894	29.38642609429818	23.24126865596335
75-79	20.35984944044724	26.17186527678268	30.56399285548503	22.90429242728505
80-84	22.899919607388046	26.035971489214273	27.61960503345207	23.44450386994561
85-89	20.758179695988105	28.49477448022308	26.332583226535615	24.4144625972532
90-94	20.287731549222816	30.13330373270634	28.905146809018774	20.673817909052072
95-99	21.87580518750988	30.17905015624422	26.6140582795981	21.331086376647796
100-104	22.599204146778746	28.719133778015483	26.613721908836794	22.06794016636898
105-109	23.485070183759348	27.863608383704857	24.106145157438334	24.545176275097464
110-114	21.373872737486167	28.099471561533985	29.249388646141323	21.277267054838525
115-119	23.161346963076582	27.88278151709941	27.146802291357623	21.809069228466385
120-124	18.49130986138161	29.38689701336401	27.847597135466597	24.274195989787785
125-129	20.725820828750283	25.43198415020973	30.536545001362303	23.30565001967769
130-134	24.798530732514607	28.87191337780155	25.91353253209818	20.416023357585665
135-139	24.039543746699362	27.562960197247815	25.044148662421666	23.353347393631154
140-144	21.318035191109047	29.140808164391117	26.585197668277882	22.95595897622195
145-149	21.14527516810129	27.100114029688083	28.857718531674355	22.896892270536277
150-154	23.209851626857187	25.811141272356043	25.44274801457158	25.53625908621519
155-159	21.529881496580792	27.234729608363523	29.937872320386422	21.297516574669263
160-164	25.304836002435326	28.363118964247153	26.080776074620488	20.251268958697032
165-169	20.375793414533234	25.70821181939581	28.274720728175424	25.641274037895528
170-174	22.55614868933133	28.618155275470837	26.28354036953692	22.542155665660918
175-179	20.84906707569351	28.895661153549888	28.326589099569112	21.92868267118749
180-184	20.407815911009752	29.24730314742121	26.518058064320815	23.82682287724822
185-189	22.760191193140727	27.174990161155232	27.488420436541972	22.576398209162065
190-194	21.119307345328313	27.850826294775153	26.493233902136293	24.53663245776024
195-199	21.8570356990289	29.345994328788965	27.019452321126437	21.7775176510557
200-204	22.941293211028924	27.59841367548967	27.239371524869572	22.22092158861183
205-209	22.318401835238873	27.223023905870008	27.606890218674636	22.851684040216487
210-214	24.0377273445883	26.830613775728157	27.175461080221062	21.95619779946248
215-219	23.841825013202552	26.979356926378532	27.04407466085418	22.134743399564734
220-224	20.71855522030603	26.937916048585393	27.779852064139178	24.5636766669694
225-229	22.619857311523052	28.228234288962668	25.832332630318444	23.31957576919584
230-234	22.27420271720301	26.997251850880115	27.421011735975863	23.307533695941014
235-239	22.608891624704412	27.265272073490284	25.381124891099965	24.744711410705335
240-244	23.73109175857998	30.702241238382594	22.875633638421615	22.691033364615816
245-249	22.01459176362554	29.16462321429172	23.890598773592203	24.930186248490536
250-254	22.366301031649126	27.534099585927592	24.76274088351144	25.33685849891184
255-259	22.335825840674627	28.137952376627613	25.995741546161845	23.53048023653592
260-264	22.27541365194372	28.81459580007467	25.444900787443952	23.465089760537655
265-269	21.6242671322038	28.5430773215469	25.53847913323982	24.294176413009474
270-274	20.97338970907293	30.35201200170876	23.662068478359586	25.01252981085872
275-279	21.952632269392616	29.41898678399279	23.11634055521358	25.51204039140102
280-284	19.80779774698864	29.992835302784144	25.74918177812312	24.4501851721041
285-289	21.59675200392881	28.951162329165697	25.8881029025433	23.56398276436219
290-294	20.92730691477374	30.661002183046243	24.02514707811538	24.38654382406464
295-299	20.42719086686109	31.81266839561238	22.067401973150886	25.692738764375644
300-301	16.422797864718408	33.38076161067776	29.234655606796032	20.961784917807805
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.0
24	0.5
25	0.75
26	0.5
27	0.5
28	0.25
29	0.75
30	1.5
31	1.75
32	2.25
33	7.5
34	23.25
35	86.5
36	299.5
37	914.75
38	1968.0
39	3259.5
40	4478.5
41	5811.25
42	7838.25
43	10614.25
44	12046.0
45	11525.75
46	9950.25
47	8071.25
48	6438.25
49	4980.75
50	3855.75
51	2958.0
52	2031.25
53	1015.0
54	405.75
55	156.75
56	74.0
57	45.75
58	30.5
59	21.5
60	17.0
61	10.25
62	12.75
63	10.0
64	5.5
65	4.25
66	4.5
67	3.5
68	3.5
69	4.0
70	3.0
71	2.0
72	2.25
73	1.75
74	2.25
75	1.25
76	1.5
77	1.25
78	1.75
79	1.25
80	0.25
81	0.25
82	0.5
83	0.75
84	0.75
85	1.5
86	0.75
87	0.75
88	0.5
89	0.5
90	0.5
91	0.0
92	0.25
93	0.25
94	0.0
95	0.0
96	0.0
97	0.25
98	0.5
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	297291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.228802404814717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.91297596672793	7.29657243623936
2	5.205761276346528	1.5855501968823467
3	5.125174350804067	2.341508024358591
4	4.3079688454559975	2.6242082525418864
5	4.524193594884083	3.4449025148809027
6	4.323270884660014	3.950294282698551
7	4.05116355851877	4.318605853958787
8	3.762953866371378	4.584422471152263
9	3.3053157954408836	4.530240102085399
>10	17.321070360148134	41.1480322639283
>50	0.08692315207524212	0.8793740213422225
>100	0.022914448135171732	0.8202575871311435
>500	0.016008968319267118	1.6595638337325893
>1k	0.025156950215860154	10.646633123434198
>5k	0.006860986422507316	6.683183715711302
>10k+	0.002286995474169105	3.486651319922135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	10011	3.3674076914538285	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8172	2.748821861408519	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5817	1.9566687185283107	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5200	1.7491279588013091	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4974	1.6731081667457137	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3813	1.2825817128671908	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3670	1.2344806940001547	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3651	1.2280896495353038	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3292	1.107332546225752	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2273	0.7645707404529568	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2244	0.7548159883750265	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1970	0.6626503997766499	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1661	0.5587118345324951	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1538	0.517338230891618	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1483	0.49883783901968104	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	979	0.32930697532047726	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	775	0.2606873400136567	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	701	0.2357959036768688	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	686	0.23075034225724964	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	560	0.18836762633244866	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	536	0.180294728061058	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	528	0.17760376197059446	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT	470	0.1580942578147337	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	461	0.15506692096296223	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	299	0.10057485763107527	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.3637076130794405E-4	0.0	0.0
2	3.3637076130794405E-4	0.0	0.0
3	3.3637076130794405E-4	0.0	0.0
4	3.3637076130794405E-4	0.0	0.0
5	3.3637076130794405E-4	0.0	0.0
6	6.727415226158881E-4	0.0	0.0
7	6.727415226158881E-4	0.0	0.0
8	6.727415226158881E-4	0.0	0.0
9	6.727415226158881E-4	0.0	0.0
10-14	0.0011436605884470098	0.0	0.0
15-19	0.001412757197493365	0.0	0.0
20-24	0.0016818538065397203	0.0	0.0
25-29	0.0016818538065397203	0.0	0.0
30-34	0.004305545744741684	0.0	0.0
35-39	0.005785577094496638	0.0	0.0
40-44	0.007871075814605891	0.0	0.0
45-49	0.011032960970900566	0.0	0.0
50-54	0.016145796542781317	0.0	0.0
55-59	0.02334413083477132	0.0	0.0
60-64	0.03377162443531758	0.0	0.0
65-69	0.05099380741428432	0.0	0.0
70-74	0.07238698783346956	0.0	0.0
75-79	0.10084395424012163	0.0	0.0
80-84	0.1419484612719524	0.0	0.0
85-89	0.20135153771893535	0.0	0.0
90-94	0.2812732306057028	0.0	0.0
95-99	0.3738424641176491	0.0	0.0
100-104	0.49621414708147915	0.0	0.0
105-109	0.6467736998429149	0.0	0.0
110-114	0.8269338795994496	0.0	0.0
115-119	1.0469203574948451	0.0	0.0
120-124	1.299736621693896	0.0	0.0
125-129	1.5850463014352942	0.0	0.0
130-134	1.9253189635744103	0.0	0.0
135-139	2.279853745992983	0.0	0.0
140-144	2.6535616618061093	0.0	0.0
145-149	3.0921891345516683	0.0	0.0
150-154	3.6006471773447557	0.0	0.0
155-159	4.1975034562095725	0.0	0.0
160-164	4.780770356317547	0.0	0.0
165-169	5.39484881816133	0.0	0.0
170-174	6.045322596378632	0.0	0.0
175-179	6.739793670175014	0.0	0.0
180-184	7.458214342176521	0.0	0.0
185-189	8.212155766572145	0.0	0.0
190-194	8.992132287893007	0.0	0.0
195-199	9.725016902630754	0.0	0.0
200-204	10.429646373418638	0.0	0.0
205-209	11.125799301021559	0.0	0.0
210-214	11.823970453192326	0.0	0.0
215-219	12.514068707091706	0.0	0.0
220-224	13.175171801366337	0.0	0.0
225-229	13.814545344460479	0.0	0.0
230-234	14.380993706503057	0.0	0.0
235-239	14.873776871819194	0.0	0.0
240-244	15.306282396708951	0.0	0.0
245-249	15.650524233831499	0.0	0.0
250-254	15.970816472748922	0.0	0.0
255-259	16.238702147054575	0.0	0.0
260-264	16.45458490166201	2.0182245678476642E-4	0.0
265-269	16.620079316225517	3.3637076130794405E-4	0.0
270-274	16.742787369950655	3.3637076130794405E-4	0.0
275-279	16.836298441594263	3.3637076130794405E-4	0.0
280-284	16.903908964617163	3.3637076130794405E-4	2.0182245678476642E-4
285-289	16.954970046183707	3.3637076130794405E-4	3.3637076130794405E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4525	0.0	291.41437	1
GGGTTAT	4535	0.0	290.12128	2
GTTATTA	4585	0.0	285.34897	4
GGTTATT	4610	0.0	285.08133	3
TTATTAG	4720	0.0	277.18753	5
TTAGTAG	4825	0.0	275.12955	8
GGGGGGA	3185	0.0	273.23392	1
TAGTAGA	4860	0.0	273.14813	9
ATTAGTA	4830	0.0	271.18015	7
TATTAGT	4845	0.0	270.645	6
GGGGAGC	3265	0.0	265.63553	3
GGAGCGA	2425	0.0	255.4639	5
GGGAGCG	2460	0.0	254.22765	4
GCGAAAG	2450	0.0	253.45918	8
CGAAAGC	2480	0.0	249.20364	9
GAGCGAA	2515	0.0	243.97615	6
AGCGAAA	2535	0.0	242.05128	7
GGGGGAG	3915	0.0	223.41635	2
GGGAGCA	1190	0.0	195.84033	4
CAAAAGC	1470	0.0	135.45918	9
>>END_MODULE