##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n02_30076_flu.3520000003bafd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409686 Sequences flagged as poor quality 0 Sequence length 301 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0334988259301 34.0 34.0 34.0 34.0 34.0 2 33.256254790254 34.0 34.0 34.0 34.0 34.0 3 33.28904575699438 34.0 34.0 34.0 34.0 34.0 4 33.28465458912435 34.0 34.0 34.0 34.0 34.0 5 33.224342545266374 34.0 34.0 34.0 34.0 34.0 6 36.671758371045144 38.0 38.0 38.0 35.0 38.0 7 35.94115249239662 38.0 38.0 38.0 34.0 38.0 8 36.601123787486024 38.0 38.0 38.0 34.0 38.0 9 36.6455773446005 38.0 38.0 38.0 35.0 38.0 10-14 36.64851959793598 38.0 38.0 38.0 35.2 38.0 15-19 36.54220598214242 38.0 38.0 38.0 35.0 38.0 20-24 36.6290500529674 38.0 38.0 38.0 34.8 38.0 25-29 36.53991251836773 38.0 38.0 38.0 34.4 38.0 30-34 36.20511904238856 38.0 37.4 38.0 32.6 38.0 35-39 36.459712072172344 38.0 37.8 38.0 34.0 38.0 40-44 35.98550157925826 38.0 37.0 38.0 32.0 38.0 45-49 36.280339088960815 38.0 37.0 38.0 34.0 38.0 50-54 36.229681268093124 38.0 37.0 38.0 34.0 38.0 55-59 35.85914529664181 38.0 37.0 38.0 32.4 38.0 60-64 36.05031072577535 38.0 37.0 38.0 32.6 38.0 65-69 35.88580669097797 38.0 37.0 38.0 31.8 38.0 70-74 35.74775559818983 38.0 37.0 38.0 31.2 38.0 75-79 35.77308670542806 38.0 37.0 38.0 31.2 38.0 80-84 35.073403045259056 38.0 36.4 38.0 27.6 38.0 85-89 35.25240305990441 38.0 36.8 38.0 28.0 38.0 90-94 35.20740323076698 38.0 36.2 38.0 27.6 38.0 95-99 34.26092129093989 38.0 34.8 38.0 23.2 38.0 100-104 34.58276436099842 38.0 35.2 38.0 24.0 38.0 105-109 34.38025024042803 37.8 34.8 38.0 23.8 38.0 110-114 33.83630927100267 37.0 34.0 38.0 23.0 38.0 115-119 33.87202833389474 37.0 34.0 38.0 23.2 38.0 120-124 33.389110196589584 37.0 32.8 38.0 21.8 38.0 125-129 32.93032273497264 37.0 31.0 38.0 21.2 38.0 130-134 32.680393276802235 37.0 30.8 38.0 20.2 38.0 135-139 31.98074720639709 36.4 28.6 38.0 17.2 38.0 140-144 30.77522199928726 35.4 26.2 37.6 13.6 38.0 145-149 30.58282489516362 35.0 26.0 37.6 11.8 38.0 150-154 29.500868958177726 34.2 23.6 37.0 10.0 38.0 155-159 29.385442021450576 34.6 23.6 37.0 10.0 37.0 160-164 28.615178453742622 33.2 22.0 37.0 10.2 37.2 165-169 27.50694629545555 31.2 20.4 36.0 9.6 37.0 170-174 26.940776106579182 29.6 20.0 35.6 9.2 37.0 175-179 26.208494798455405 28.6 19.6 35.2 9.0 37.0 180-184 25.325955976040184 26.8 17.4 35.0 9.0 36.8 185-189 24.594045195588816 26.0 16.0 35.0 8.2 36.2 190-194 23.70291100989538 24.8 15.2 34.0 8.0 36.0 195-199 22.92137637117207 23.6 14.0 33.0 8.0 36.0 200-204 22.176829571916052 23.0 12.2 31.4 8.0 36.0 205-209 21.30587327855968 21.8 9.6 30.2 8.0 35.2 210-214 20.161787320045107 20.2 9.0 28.2 8.0 35.0 215-219 19.688515594870218 19.6 9.0 27.6 8.0 34.0 220-224 19.091860595675712 18.8 9.0 26.2 8.0 33.4 225-229 18.369753909091354 17.2 9.0 25.6 8.0 32.4 230-234 17.488166058884122 16.0 9.0 24.6 8.0 31.2 235-239 16.55920583080701 14.8 8.8 23.0 7.8 29.4 240-244 15.834043145238061 14.0 8.0 21.8 7.0 28.8 245-249 15.230834346304244 13.2 8.0 20.8 7.0 27.4 250-254 14.523764541624562 12.4 8.0 19.6 7.0 26.2 255-259 14.01949981205118 11.8 8.0 19.0 7.0 25.0 260-264 13.438496311809534 10.6 8.0 17.8 7.0 23.8 265-269 13.047709221208436 10.2 8.0 17.0 7.0 23.4 270-274 12.542922140370916 9.4 8.0 15.8 7.0 22.0 275-279 12.035701488457011 9.0 7.2 14.4 7.0 21.2 280-284 11.949190843719338 8.8 7.0 14.4 7.0 21.0 285-289 11.634407814765455 8.2 7.4 13.6 7.0 20.4 290-294 11.28560019136607 8.0 7.0 13.0 7.0 19.2 295-299 10.936406418574226 8.0 7.0 13.0 7.0 19.0 300-301 10.59300049306054 8.0 7.0 12.5 7.0 17.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35612048333315727 1101 2 0.265073035161727 1101 3 0.23892835093562326 1101 4 0.22200775124306915 1101 5 0.19573111062179294 1101 6 0.2857440126201354 1101 7 1.1000486738162678 1101 8 0.5831991375082168 1101 9 0.3522257871402772 1101 10-14 0.3739005298162681 1101 15-19 0.4971806121363542 1101 20-24 0.43188361781817264 1101 25-29 0.37296412804789725 1101 30-34 0.734634368251804 1101 35-39 0.5165584435342581 1101 40-44 0.8080479100085327 1101 45-49 0.3710856789670558 1101 50-54 0.5683315282102299 1101 55-59 0.6815088428427529 1101 60-64 0.6088271828143235 1101 65-69 0.6760158087368282 1101 70-74 0.6423950477932365 1101 75-79 0.5875388161768527 1101 80-84 1.0735044164915166 1101 85-89 0.7641663494168043 1101 90-94 0.8144949111570554 1101 95-99 1.445141047666766 1101 100-104 0.9916316821907856 1101 105-109 0.9049053079919389 1101 110-114 0.9062986056513225 1101 115-119 0.9439843429722217 1101 120-124 1.2215449248635082 1101 125-129 1.3511408379079484 1101 130-134 1.111871671153601 1101 135-139 1.0197740760571037 1101 140-144 1.6373854730927562 1101 145-149 1.8140546442441803 1101 150-154 1.5335719341539402 1101 155-159 1.282364184405342 1101 160-164 1.5669250269399875 1101 165-169 1.1368736768457985 1101 170-174 1.1216937950128312 1101 175-179 1.3480127875739747 1101 180-184 0.9307846992783411 1101 185-189 0.9645391889016217 1101 190-194 1.2941400607328752 1101 195-199 1.2261991855611463 1101 200-204 1.4993056281634658 1101 205-209 1.2643692773043824 1101 210-214 1.5455751069076342 1101 215-219 1.2304120179269553 1101 220-224 0.8672920514028277 1101 225-229 0.8643250187895077 1101 230-234 1.0058165041651712 1101 235-239 0.7113500585589598 1101 240-244 0.6391479822257331 1101 245-249 0.6598490356384072 1101 250-254 0.3343896950658021 1101 255-259 0.48961697250907044 1101 260-264 0.29766521880527996 1101 265-269 0.46722562425378555 1101 270-274 0.318894946960274 1101 275-279 0.22886797441287143 1101 280-284 0.3856222858513956 1101 285-289 0.2800005097052001 1101 290-294 0.01843132924489943 1101 295-299 -0.03950822132896903 1101 300-301 -0.12271275254067326 1102 1 0.029081644378756266 1102 2 -0.07893894911578059 1102 3 0.08702041822349571 1102 4 -0.01386743085747355 1102 5 0.2274544002509984 1102 6 0.19561454943880818 1102 7 0.9458112284464377 1102 8 0.21439570989490164 1102 9 0.35669755811963455 1102 10-14 0.0888207491615347 1102 15-19 0.16914331244695546 1102 20-24 0.07546130947039842 1102 25-29 0.1634766895497748 1102 30-34 0.4920035994439118 1102 35-39 0.1452660793881364 1102 40-44 0.45789157840687267 1102 45-49 0.13419760552212523 1102 50-54 0.11930387641395868 1102 55-59 0.40284415523108663 1102 60-64 0.2872833137617903 1102 65-69 0.2785861751368728 1102 70-74 0.13230495956037203 1102 75-79 0.391016593038394 1102 80-84 0.7871918494922596 1102 85-89 0.3171043527702011 1102 90-94 0.32183459395852054 1102 95-99 0.9668841965631643 1102 100-104 0.5232268142652217 1102 105-109 0.9758867268644309 1102 110-114 0.8294277802204633 1102 115-119 0.5071608636835734 1102 120-124 1.0029940329144154 1102 125-129 0.7550999786682411 1102 130-134 0.9047931136648018 1102 135-139 0.9671714125789066 1102 140-144 1.9061212142763466 1102 145-149 1.6254232157512831 1102 150-154 1.5237446891634292 1102 155-159 1.1121431212130162 1102 160-164 1.269843102308993 1102 165-169 1.4931183512637674 1102 170-174 1.3492985200066414 1102 175-179 1.123519920418044 1102 180-184 0.965036437823187 1102 185-189 1.0150092144644098 1102 190-194 1.578957685317139 1102 195-199 1.0499368508259828 1102 200-204 0.9185340715817318 1102 205-209 0.8553697308378183 1102 210-214 1.4231952932686447 1102 215-219 1.4245689954701746 1102 220-224 0.9590477068273664 1102 225-229 1.258421181621756 1102 230-234 1.087538075864682 1102 235-239 0.6791378461403887 1102 240-244 0.6420018070243891 1102 245-249 0.17440574106156959 1102 250-254 0.7018751270220438 1102 255-259 0.6834647227083508 1102 260-264 0.5853995444148001 1102 265-269 0.592540348972733 1102 270-274 0.4092704176730084 1102 275-279 0.20307635241237598 1102 280-284 0.23998376574474634 1102 285-289 0.32138474873106837 1102 290-294 0.055725521268207956 1102 295-299 0.2069796432157105 1102 300-301 0.06581972320216067 1103 1 -0.055686992294639026 1103 2 0.005462232086529184 1103 3 0.16923941500324702 1103 4 0.06639987629521471 1103 5 0.17397459693989248 1103 6 0.1804352979790096 1103 7 1.030705969156493 1103 8 0.41144287113436206 1103 9 0.47349411472782066 1103 10-14 0.12620717201592413 1103 15-19 0.20657666938652142 1103 20-24 0.2368731402003661 1103 25-29 0.15344031145814796 1103 30-34 0.6049186571010239 1103 35-39 0.19707947213790789 1103 40-44 0.4690784261734109 1103 45-49 0.16151057068734787 1103 50-54 0.1888000615580694 1103 55-59 0.4284831937003375 1103 60-64 0.23245845640133211 1103 65-69 0.43879759565291465 1103 70-74 0.4329765679463691 1103 75-79 0.37737297139727133 1103 80-84 1.056324420745213 1103 85-89 0.5724643589167258 1103 90-94 0.6234530682833963 1103 95-99 1.2710543150842568 1103 100-104 0.8402715703415993 1103 105-109 0.8272192540227223 1103 110-114 0.7011298673675412 1103 115-119 0.828286546816706 1103 120-124 1.1846145783519546 1103 125-129 1.2187217200063074 1103 130-134 1.151636003067388 1103 135-139 1.2434914128461543 1103 140-144 1.7212947868788646 1103 145-149 1.8545457320863505 1103 150-154 1.963408166762001 1103 155-159 0.9351449985764226 1103 160-164 1.0649060040046336 1103 165-169 1.8030635335884924 1103 170-174 2.10640026508608 1103 175-179 1.788487717956766 1103 180-184 1.3035399253096536 1103 185-189 1.4606371624594452 1103 190-194 1.3383618729733016 1103 195-199 1.8816890121322523 1103 200-204 1.6837921589426017 1103 205-209 1.3705000439098818 1103 210-214 1.879001757812535 1103 215-219 1.89407587022799 1103 220-224 1.3944679088610634 1103 225-229 0.9658339276498964 1103 230-234 1.4068670687694507 1103 235-239 1.249569539508819 1103 240-244 0.7999077785098478 1103 245-249 1.1524217153530483 1103 250-254 0.8256880788252463 1103 255-259 0.9673284167716076 1103 260-264 0.7432345301842513 1103 265-269 0.7383690612751419 1103 270-274 0.6923213670443236 1103 275-279 0.39045559318256373 1103 280-284 0.6117675282215291 1103 285-289 0.5655689307751874 1103 290-294 0.3052224413232594 1103 295-299 0.2044178104814396 1103 300-301 0.013697834881901017 2116 1 0.14552181074171955 2116 2 0.11847797372573865 2116 3 0.16590285830639573 2116 4 0.15922273341899995 2116 5 0.20279380295787064 2116 6 0.21624300084440762 2116 7 1.0064890253890297 2116 8 0.25557002239576576 2116 9 0.1917490596984166 2116 10-14 0.1977274621019518 2116 15-19 0.23222735634674052 2116 20-24 0.18141754877712657 2116 25-29 0.16374984613405985 2116 30-34 0.423670289293824 2116 35-39 0.03063577100461856 2116 40-44 0.5081824701735727 2116 45-49 0.32895598974268125 2116 50-54 0.3254844136161097 2116 55-59 0.48720241982609735 2116 60-64 0.37158611244312567 2116 65-69 0.6066788779786805 2116 70-74 0.5577714644079919 2116 75-79 0.4253411260119222 2116 80-84 1.0124837845049939 2116 85-89 0.8889928714999442 2116 90-94 0.7420335757503977 2116 95-99 1.4535562069678107 2116 100-104 0.6703768399986245 2116 105-109 0.8624340693690442 2116 110-114 1.0338534037338434 2116 115-119 1.187535667205573 2116 120-124 1.2208975257021137 2116 125-129 1.2301432733782818 2116 130-134 1.3111496116965355 2116 135-139 1.748367424597106 2116 140-144 2.19568006030714 2116 145-149 2.151415342721279 2116 150-154 2.401435568411177 2116 155-159 2.0579998696238064 2116 160-164 2.147877383373384 2116 165-169 1.75029571004303 2116 170-174 1.8604569056181965 2116 175-179 1.9006467513148202 2116 180-184 2.08759019976835 2116 185-189 1.978475434158117 2116 190-194 1.841644947978665 2116 195-199 1.1099568811496638 2116 200-204 0.9050017796397682 2116 205-209 1.3055343898354685 2116 210-214 2.2595489397612525 2116 215-219 2.183066234345695 2116 220-224 1.5729974710537604 2116 225-229 1.1709236158624847 2116 230-234 1.6178702801739853 2116 235-239 1.1262409258026551 2116 240-244 1.0193484150838152 2116 245-249 1.0043745480054138 2116 250-254 1.094160496539784 2116 255-259 1.6755217952209023 2116 260-264 0.6808143042595667 2116 265-269 0.9305850972722425 2116 270-274 1.0340642027086862 2116 275-279 0.685609284211905 2116 280-284 0.476514416312968 2116 285-289 0.30112491092104854 2116 290-294 0.39017489601609157 2116 295-299 0.2486062028685172 2116 300-301 0.2599851959317103 1117 1 0.21892311345021653 1117 2 0.11504997281933527 1117 3 0.2205660129191216 1117 4 0.1268696970314167 1117 5 0.0911307624982598 1117 6 0.11696301733697823 1117 7 1.039376196112677 1117 8 0.36061218237709625 1117 9 0.43003005013851947 1117 10-14 0.29453543441897523 1117 15-19 0.2855619050415612 1117 20-24 0.2496870727927245 1117 25-29 0.31470898783895507 1117 30-34 0.6253892307026092 1117 35-39 0.25206401384746613 1117 40-44 0.6236747512083127 1117 45-49 0.37136983395947 1117 50-54 0.29233656489113 1117 55-59 0.4260262338689742 1117 60-64 0.4334001928035178 1117 65-69 0.5812884998933541 1117 70-74 0.4754149352759356 1117 75-79 0.6395220092844127 1117 80-84 1.0188567653153413 1117 85-89 0.7002856810465445 1117 90-94 0.5410458957675743 1117 95-99 1.1862438653126546 1117 100-104 0.6267217936643874 1117 105-109 0.6119347772887238 1117 110-114 0.9609371665611519 1117 115-119 0.9922866515631981 1117 120-124 1.2799865838707873 1117 125-129 1.1123870124208608 1117 130-134 0.9615129423095112 1117 135-139 1.1901266773883208 1117 140-144 1.55175752086042 1117 145-149 1.720825545964935 1117 150-154 2.190994136624475 1117 155-159 1.6087559210165558 1117 160-164 1.5849045637404124 1117 165-169 1.5032908662027786 1117 170-174 1.0845863177637405 1117 175-179 1.3875564562034697 1117 180-184 1.6109268441098479 1117 185-189 1.197992379080091 1117 190-194 -0.9345747439442356 1117 195-199 0.8664202473715896 1117 200-204 1.6484762255286363 1117 205-209 1.350970224712139 1117 210-214 1.7092685831264838 1117 215-219 1.4394554862339035 1117 220-224 1.3218430764145275 1117 225-229 1.6036976557411045 1117 230-234 1.3215618555039654 1117 235-239 1.275038484629846 1117 240-244 0.7082212127380565 1117 245-249 1.2940068897968668 1117 250-254 0.7650272594131042 1117 255-259 0.6259686109454083 1117 260-264 0.526497186915277 1117 265-269 0.396456554053362 1117 270-274 0.41062516132222093 1117 275-279 0.25195422083988994 1117 280-284 0.19148517096726358 1117 285-289 0.5661253964977178 1117 290-294 0.1341642235520375 1117 295-299 0.128768095833788 1117 300-301 0.06579243732805828 2117 1 0.09483141537934614 2117 2 0.0183723284314965 2117 3 0.12138043223286843 2117 4 0.05754670783534266 2117 5 0.14118224119070533 2117 6 0.292939297539462 2117 7 0.9637371673440072 2117 8 0.41158103776816546 2117 9 0.29926922146356816 2117 10-14 0.08922553929031096 2117 15-19 0.2557953411122966 2117 20-24 0.2988165549038868 2117 25-29 0.1284148597098138 2117 30-34 0.4609755268106923 2117 35-39 0.26716179036395005 2117 40-44 0.5149642924539677 2117 45-49 0.19081030017161993 2117 50-54 0.316182749198056 2117 55-59 0.4820779501553787 2117 60-64 0.31223684035266785 2117 65-69 0.35310325579854407 2117 70-74 0.4566011231467684 2117 75-79 0.5706989573899222 2117 80-84 0.6794092372825489 2117 85-89 0.3433766465360222 2117 90-94 0.30454783281837194 2117 95-99 1.1394170647133919 2117 100-104 0.31755099660262687 2117 105-109 0.411762851655638 2117 110-114 0.6663889580856477 2117 115-119 0.6536571727519558 2117 120-124 0.9426836713647617 2117 125-129 0.7442447332347513 2117 130-134 1.0671927580421894 2117 135-139 1.34735151387747 2117 140-144 1.8285752796893462 2117 145-149 1.7129898949904643 2117 150-154 1.9290184108079131 2117 155-159 1.85415979775809 2117 160-164 1.9011682006440367 2117 165-169 1.4731246824238546 2117 170-174 1.0189971837277483 2117 175-179 2.0150860069528846 2117 180-184 1.3755118136516202 2117 185-189 1.5835770252300811 2117 190-194 1.3299356474918227 2117 195-199 1.7577470776700999 2117 200-204 1.8386375254316185 2117 205-209 1.6997383660013519 2117 210-214 2.0111633949982597 2117 215-219 1.8507705688308107 2117 220-224 1.2870725195866726 2117 225-229 0.8704645298624989 2117 230-234 0.8630546715864362 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160-164 -1.1847029349143554 2110 165-169 -2.105993505885028 2110 170-174 -1.7383851022719377 2110 175-179 -1.2658157826820506 2110 180-184 -1.5282774592651904 2110 185-189 -1.4598317617131968 2110 190-194 -1.4686586896326297 2110 195-199 -2.063705245101069 2110 200-204 -1.692980084656572 2110 205-209 -1.4770969399863318 2110 210-214 -1.464978046162333 2110 215-219 -1.3649039046531612 2110 220-224 -1.0871542568916475 2110 225-229 -1.737516088850402 2110 230-234 -1.0873623177106175 2110 235-239 -1.2702511767119251 2110 240-244 -0.8619308278513671 2110 245-249 -0.909918632890399 2110 250-254 -0.6572604277944034 2110 255-259 -0.9973280404951321 2110 260-264 -0.9188714058478133 2110 265-269 -0.8111643171118867 2110 270-274 -0.6191245157379317 2110 275-279 -0.327110110814667 2110 280-284 -0.4976328170792943 2110 285-289 -0.5171426698861534 2110 290-294 -0.5785169155726582 2110 295-299 -0.39197440271064643 2110 300-301 -0.5820932190503658 2109 1 -5.378062260135152E-4 2109 2 -0.002631788474140251 2109 3 -0.11077223881945741 2109 4 -0.009178591553542503 2109 5 -0.2032844533388456 2109 6 -0.34371251623555565 2109 7 0.4295686605203386 2109 8 0.054414791787166905 2109 9 -0.03231198622790998 2109 10-14 -0.14652786102363535 2109 15-19 -0.07057457487123742 2109 20-24 -0.17405909216772386 2109 25-29 -0.2663068789341452 2109 30-34 -3.9789545088296663 2109 35-39 -0.2738387723785465 2109 40-44 -0.10942549393424628 2109 45-49 -0.7075584047637236 2109 50-54 -0.4334828961951942 2109 55-59 -0.14673611116710106 2109 60-64 -0.1260051323395075 2109 65-69 -0.21285707579171032 2109 70-74 -0.3796923125113594 2109 75-79 -0.6307194169753458 2109 80-84 -0.474197251039719 2109 85-89 -0.5814284503971905 2109 90-94 -0.3474109059802615 2109 95-99 -0.25428564928507313 2109 100-104 -0.8458712346878272 2109 105-109 -0.5139651758825536 2109 110-114 -0.8211489283061795 2109 115-119 -0.5478490684243411 2109 120-124 -0.2760750700665753 2109 125-129 -1.0386681338230908 2109 130-134 -0.9397710980931784 2109 135-139 -1.1941931978239566 2109 140-144 -0.6590419171948376 2109 145-149 -0.8183882646770222 2109 150-154 -0.865992926314334 2109 155-159 -1.926501112041862 2109 160-164 -1.8927188032951605 2109 165-169 -1.5705255418504294 2109 170-174 -1.3502240925459077 2109 175-179 -1.4864825966230057 2109 180-184 -1.428487113517651 2109 185-189 -1.3603952128067078 2109 190-194 -1.0110278927179372 2109 195-199 -2.001624394905253 2109 200-204 -1.8138303143282783 2109 205-209 -1.5028189860154733 2109 210-214 -1.9697066509754926 2109 215-219 -0.894584666484409 2109 220-224 -0.9862554605626705 2109 225-229 -0.8868132773504733 2109 230-234 -1.1299994076384934 2109 235-239 -1.1736058986724665 2109 240-244 -1.0769382465817792 2109 245-249 -1.029319649760076 2109 250-254 -1.0833071543695407 2109 255-259 -0.7204268268365421 2109 260-264 -0.5497864785242861 2109 265-269 -0.5601940419713625 2109 270-274 -0.6405989683050333 2109 275-279 -0.5114670427358572 2109 280-284 -0.8282617205884009 2109 285-289 -0.9990073654473566 2109 290-294 -0.38930230223959406 2109 295-299 -0.37165986208368196 2109 300-301 -0.3804074363510299 2108 1 0.011466153671264578 2108 2 0.031212489482626893 2108 3 -0.30505824381853586 2108 4 0.03484315618899103 2108 5 0.03378812503967765 2108 6 -0.2940278151335747 2108 7 0.9002968629454884 2108 8 0.19589688426094654 2108 9 -0.29452340704538926 2108 10-14 -0.2109093856174553 2108 15-19 -0.15668215764085147 2108 20-24 -0.12278943133608067 2108 25-29 -0.07865044923024556 2108 30-34 0.2369674808602511 2108 35-39 -0.17894685639042507 2108 40-44 -0.24567231529934475 2108 45-49 -0.30213297852015586 2108 50-54 -0.2890528987222609 2108 55-59 -0.1352667540832755 2108 60-64 -0.11821077344171016 2108 65-69 -0.30722089427362675 2108 70-74 -0.33390112119777626 2108 75-79 -0.4693359216070405 2108 80-84 -0.11335419658112755 2108 85-89 -0.49646930456071203 2108 90-94 -0.30035785186360897 2108 95-99 -0.33285925224949153 2108 100-104 -0.8393243935566019 2108 105-109 -0.8190160465512122 2108 110-114 -1.0582917306424235 2108 115-119 -0.7829713890509211 2108 120-124 -0.7298335947296124 2108 125-129 -0.5432069200495135 2108 130-134 -0.9264685787878548 2108 135-139 -1.3396083323378676 2108 140-144 -0.7719853935809908 2108 145-149 -1.0584005747199647 2108 150-154 -0.7432190966649799 2108 155-159 -2.064078185337493 2108 160-164 -1.5377081737899267 2108 165-169 -1.1330688167388523 2108 170-174 -1.788915658415064 2108 175-179 -1.1011837569863339 2108 180-184 -1.708976446064785 2108 185-189 -1.431665690971812 2108 190-194 -1.4805737972517221 2108 195-199 -1.4969695139648849 2108 200-204 -1.5223837091395467 2108 205-209 -1.2182256386095425 2108 210-214 -1.1104216176024337 2108 215-219 -1.0253685309871976 2108 220-224 -0.7109156625107609 2108 225-229 -0.8888639503578695 2108 230-234 -0.9585959042803367 2108 235-239 -0.8052412078121716 2108 240-244 -0.5278507827258494 2108 245-249 -0.9557981684849359 2108 250-254 -0.8181923648871212 2108 255-259 -0.8308792709555348 2108 260-264 -0.5295311201944504 2108 265-269 -0.4239625664592577 2108 270-274 -0.4457356589885162 2108 275-279 -0.36095424938194576 2108 280-284 -0.843689473730338 2108 285-289 -1.2278512305673175 2108 290-294 -0.9046150448854693 2108 295-299 -0.6038426178485938 2108 300-301 2.4794799217519543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 11.0 10 403.0 11 988.0 12 926.0 13 784.0 14 1163.0 15 1940.0 16 2854.0 17 4058.0 18 5001.0 19 5916.0 20 7509.0 21 9727.0 22 13090.0 23 17103.0 24 22776.0 25 30908.0 26 42122.0 27 53581.0 28 62056.0 29 59160.0 30 42125.0 31 19707.0 32 5151.0 33 601.0 34 21.0 35 2.0 36 1.0 37 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.410607147913282 19.91256718560068 14.26214222599747 35.414683440488574 2 48.611131451892426 22.846765571681726 14.87090112915745 13.671201847268396 3 48.419033113164716 21.794252183379466 16.99740777082937 12.78930693262645 4 48.48151999336076 20.54475866883418 17.488027416118687 13.485693921686364 5 29.670772249967047 20.560868567634724 36.63708303432385 13.131276148074377 6 28.460821214295827 20.37853380393765 36.661980150651964 14.49866483111456 7 15.886557021719073 50.875548590872036 18.826369463442735 14.41152492396616 8 27.67387706682679 19.09901729617317 38.08795028387594 15.139155353124101 9 14.411769013341925 17.97254482701386 39.05991417817548 28.555771981468737 10-14 26.94151130377899 35.173913680233156 22.731408932694798 15.15316608329306 15-19 21.32340377752718 36.42370010202936 20.643566048144187 21.609330072299272 20-24 25.71740308431335 31.28005350439117 26.35491571593855 16.64762769535693 25-29 18.96598858638079 25.218972578999526 39.21989035505241 16.59514847956728 30-34 18.569050443510395 28.46472664430808 32.19138559775047 20.774837314431053 35-39 20.164467421391016 30.255708030052286 27.637019571086146 21.94280497747055 40-44 19.262850085187193 29.433322105222047 29.311326235214285 21.992501574376476 45-49 21.28967062579634 30.352416240730705 25.841595758703008 22.516317374769944 50-54 21.735133736568983 30.489448016285642 27.233442197194925 20.54197604995045 55-59 21.401805285023165 26.767133853731885 30.11594245348877 21.715118407756183 60-64 21.75109718174407 25.364059303954733 29.478088096737505 23.406755417563694 65-69 19.44972491127351 26.389869314548214 29.20636780363498 24.954037970543293 70-74 20.130978359035943 25.305575489521242 30.015475266423554 24.547970885019257 75-79 19.678876017242473 25.36523093295841 31.37890970157633 23.576983348222786 80-84 22.643195032293022 25.779206514257258 27.08728147898635 24.49031697446337 85-89 20.67153869060695 27.679051761593026 25.904326728274825 25.745082819525194 90-94 19.37957362467841 29.889671602153843 29.560004491244513 21.17075028192323 95-99 21.94729622198464 29.803166327382435 26.77460298863032 21.474934462002608 100-104 22.1409079148421 27.99514750320978 26.95986682483658 22.904077757111544 105-109 23.1269801750609 27.9801115976626 23.301406442983165 25.591501784293335 110-114 20.80193123514106 28.21170359738922 29.568498801521166 21.417866365948555 115-119 23.67037194339079 27.312771244318817 27.08142333396796 21.935433478322423 120-124 17.585809620050476 29.348183730954926 27.76145633485157 25.304550314143025 125-129 19.57694429392266 23.96518309144076 32.25294493831862 24.20492767631796 130-134 25.252315187729142 28.352396713580646 26.162329198459304 20.23295890023091 135-139 24.231386964650977 26.96694541673379 24.594201412789307 24.207466205825927 140-144 20.205474436519676 28.734689493905087 27.24989382112154 23.809942248453694 145-149 20.937400838691094 26.540423641520576 29.74956430046426 22.77261121932407 150-154 23.12556445668146 24.881396972315382 25.034538646670867 26.95849992433229 155-159 20.79402273936625 25.78213558676645 31.36963430529723 22.054207368570076 160-164 25.497771463999257 28.65711789028671 25.898322129630984 19.94678851608305 165-169 20.036174045488497 25.159658860688427 28.61010627651421 26.194060817308866 170-174 21.947247404109486 28.720581127985824 26.111802697675778 23.22036877022891 175-179 18.950318048456623 28.878409318356006 29.242248941872557 22.92902369131481 180-184 19.131481183150022 29.80804811489775 26.733742427127115 24.32672827482511 185-189 21.391944074242225 27.28997329662229 27.953261766328357 23.364820862807125 190-194 19.351698617965955 27.221823542908467 26.884492025600093 26.541985813525482 195-199 20.48310169251573 30.014889451921718 27.12272325634754 22.37928559921501 200-204 22.078176945270283 26.598663366578307 27.897414117153136 23.425745570998277 205-209 20.527233051654193 27.063555991661907 28.812846912025307 23.596364044658593 210-214 23.412320655331158 25.68225421420307 28.38515350780842 22.520271622657354 215-219 23.029100335378804 26.08446468759001 28.433385568459745 22.45304940857144 220-224 18.599903340607195 26.028568220539633 29.486533589138997 25.88499484971417 225-229 21.64428367090894 28.0425496599835 26.76557168172698 23.54759498738058 230-234 21.017950332693818 26.31547087281479 28.979022959046684 23.687555835444705 235-239 22.10766294186279 26.04126086807946 25.936741797376527 25.914334392681226 240-244 22.453830494573893 30.870959710607636 23.156807896779487 23.518401898038984 245-249 20.621988547326488 28.7170662409748 24.69003090171497 25.970914309983744 250-254 21.227720742226975 25.961980638830713 26.47832730432575 26.33197131461656 255-259 21.447986994918057 26.694981034255505 27.875543709084518 23.98148826174192 260-264 20.907963659973735 27.730017623252927 27.321363190345778 24.040655526427557 265-269 20.677250381999873 27.47157579219207 27.328881143119364 24.522292682688693 270-274 19.59446991110265 29.6723832398471 25.0982947916209 25.63485205742935 275-279 21.05095121629736 27.817401619777097 24.517850256049755 26.613796907875788 280-284 18.33623799690495 29.146907631698422 27.665041031424064 24.851813339972566 285-289 21.16552676928184 26.73178971212099 29.11727518148045 22.98540833711672 290-294 20.672270958734252 29.65056164965364 25.864588977900148 23.812578413711964 295-299 20.312092675854192 30.57556274805583 23.253076746581527 25.859267829508454 300-301 16.120150554326973 31.26223497996026 30.67776296968898 21.939851496023785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.25 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.25 21 0.25 22 0.0 23 0.0 24 0.25 25 0.75 26 0.0 27 0.75 28 1.0 29 1.25 30 2.5 31 3.25 32 10.0 33 16.75 34 50.25 35 180.75 36 630.5 37 1856.75 38 3806.75 39 5543.75 40 6910.5 41 8305.75 42 10993.0 43 14653.5 44 16150.25 45 14635.0 46 12183.5 47 9703.0 48 7839.5 49 6376.5 50 5073.75 51 4172.75 52 3066.75 53 1736.5 54 800.5 55 362.75 56 226.25 57 158.25 58 141.0 59 128.0 60 135.75 61 148.75 62 141.25 63 115.0 64 90.5 65 65.0 66 45.25 67 31.25 68 22.5 69 17.75 70 12.5 71 10.0 72 5.5 73 4.25 74 4.25 75 5.0 76 4.5 77 2.5 78 1.25 79 2.5 80 1.5 81 1.25 82 1.5 83 1.0 84 1.0 85 1.25 86 1.0 87 1.0 88 0.75 89 1.0 90 0.5 91 0.5 92 0.75 93 1.75 94 1.25 95 1.25 96 0.0 97 0.25 98 0.25 99 1.25 100 1.3333333333333333 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 409686.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.317467887825142 #Duplication Level Percentage of deduplicated Percentage of total 1 60.48751324179844 9.265155416956455 2 4.724435120486525 1.4473276649233129 3 3.5731610049528797 1.641953368541842 4 3.5436712987922467 2.171202852970314 5 3.361223387978988 2.5742715654587487 6 2.7243403193515965 2.5037997214305223 7 2.4567840255910367 2.634219729351874 8 2.3503799849301945 2.8801495954684158 9 2.0014322533564477 2.759118684340041 >10 14.236703328575395 39.334330652385766 >50 0.4529377440424019 4.478091197516984 >100 0.04966007533224021 1.3022135130850858 >500 0.008208307567803403 1.0780132005369243 >1k 0.018058276649042575 8.116405895391088 >5k 0.0065666460541973 7.376104784506205 >10k+ 0.004924984540647975 10.437642157136452 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 20084 4.902291022880938 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 10857 2.6500783526896208 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 10567 2.57929243371753 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 9833 2.4001308319054107 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 7792 1.9019444159673506 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG 6234 1.5216531685241867 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 5474 1.3361452429421556 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 4603 1.1235433966501174 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 4547 1.109874391607231 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 3907 0.9536571911170996 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 3882 0.9475549567229538 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA 3272 0.7986604375057971 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 3025 0.738370361691637 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 2407 0.5875231274683538 No Hit GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 2232 0.5448074867093335 No Hit GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 1569 0.3829762305765879 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 1463 0.3571027567454099 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 1370 0.3344024447991877 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 998 0.24360119701429878 Illumina Single End PCR Primer 1 (97% over 34bp) GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA 958 0.23383762198366553 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT 928 0.22651494071069062 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 736 0.1796497805636512 No Hit GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 667 0.1628076136358089 No Hit GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC 426 0.10398207407624376 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.881787515316608E-4 0.0 0.0 2 4.881787515316608E-4 0.0 0.0 3 4.881787515316608E-4 0.0 0.0 4 4.881787515316608E-4 0.0 0.0 5 4.881787515316608E-4 0.0 0.0 6 4.881787515316608E-4 0.0 0.0 7 4.881787515316608E-4 0.0 0.0 8 7.322681272974912E-4 0.0 0.0 9 7.322681272974912E-4 0.0 0.0 10-14 0.0019038971309734772 0.0 0.0 15-19 0.0026361652582709686 0.0 0.0 20-24 0.0034172512607216258 0.0 0.0 25-29 0.0037589763867937886 0.0 0.0 30-34 0.005662873517767265 0.0 0.0 35-39 0.00585814501837993 0.0 0.0 40-44 0.006541595270524255 4.8817875153166085E-5 0.0 45-49 0.007615588523893909 2.440893757658304E-4 0.0 50-54 0.00922657840394839 2.440893757658304E-4 0.0 55-59 0.011667472161606693 2.440893757658304E-4 0.0 60-64 0.016207534550851138 2.440893757658304E-4 0.0 65-69 0.021235775691627247 2.440893757658304E-4 0.0 70-74 0.030511171970728806 2.440893757658304E-4 0.0 75-79 0.041592829630497506 2.440893757658304E-4 0.0 80-84 0.05882553955956513 2.440893757658304E-4 0.0 85-89 0.08020776887665189 2.440893757658304E-4 0.0 90-94 0.10573951758175774 2.440893757658304E-4 0.0 95-99 0.13561605717549538 2.440893757658304E-4 0.0 100-104 0.17164364903853196 2.440893757658304E-4 0.0 105-109 0.21221130329081297 2.440893757658304E-4 0.0 110-114 0.2573678378074916 2.440893757658304E-4 0.0 115-119 0.3098958714722983 2.440893757658304E-4 0.0 120-124 0.3726268410441167 2.440893757658304E-4 0.0 125-129 0.44521902139687464 2.440893757658304E-4 0.0 130-134 0.5365572658084485 2.440893757658304E-4 0.0 135-139 0.6312151257304375 2.440893757658304E-4 0.0 140-144 0.7383215438164838 2.440893757658304E-4 0.0 145-149 0.8713502536088613 2.440893757658304E-4 0.0 150-154 1.0257611927183259 2.440893757658304E-4 0.0 155-159 1.2157115449392948 2.440893757658304E-4 0.0 160-164 1.406491801037868 2.440893757658304E-4 0.0 165-169 1.616945660823167 2.440893757658304E-4 0.0 170-174 1.8366260990124144 2.440893757658304E-4 0.0 175-179 2.089307420805202 2.440893757658304E-4 0.0 180-184 2.3599049027792014 2.440893757658304E-4 0.0 185-189 2.6840555937962245 2.440893757658304E-4 0.0 190-194 3.0193367603481693 2.440893757658304E-4 0.0 195-199 3.3500778645108698 2.440893757658304E-4 0.0 200-204 3.686140117065265 2.440893757658304E-4 0.0 205-209 4.00863100032708 2.440893757658304E-4 0.0 210-214 4.336931210732121 2.440893757658304E-4 0.0 215-219 4.685149114199655 2.929072509189965E-4 0.0 220-224 5.031951299287747 4.881787515316608E-4 0.0 225-229 5.368355276968215 4.881787515316608E-4 0.0 230-234 5.684304564959506 4.881787515316608E-4 0.0 235-239 5.976967726502737 4.881787515316608E-4 0.0 240-244 6.232871028055633 4.881787515316608E-4 0.0 245-249 6.4476696787295635 6.34632376991159E-4 0.0 250-254 6.650117406989743 7.322681272974912E-4 0.0 255-259 6.813754924503155 7.322681272974912E-4 0.0 260-264 6.961917175593015 7.322681272974912E-4 0.0 265-269 7.086939753860273 7.322681272974912E-4 0.0 270-274 7.1837456002890026 7.322681272974912E-4 0.0 275-279 7.2486245563675595 7.322681272974912E-4 0.0 280-284 7.300517957655376 7.322681272974912E-4 2.440893757658304E-4 285-289 7.3437217771659276 7.322681272974912E-4 2.440893757658304E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 7325 0.0 291.77817 1 GGGTTAT 7420 0.0 287.64487 2 GTTATTA 7435 0.0 286.27103 4 GGTTATT 7515 0.0 283.6161 3 TTATTAG 7595 0.0 278.88083 5 TTAGTAG 7735 0.0 277.2657 8 TAGTAGA 7800 0.0 274.00958 9 GGGGGGA 5855 0.0 272.3271 1 GGGGAGC 5860 0.0 271.0879 3 TATTAGT 8020 0.0 265.38965 6 GGGAGCG 4350 0.0 265.16092 4 GGAGCGA 4340 0.0 264.41245 5 ATTAGTA 8060 0.0 263.34058 7 GCGAAAG 4430 0.0 260.37247 8 CGAAAGC 4460 0.0 258.29034 9 GAGCGAA 4490 0.0 253.27953 6 AGCGAAA 4545 0.0 249.56546 7 GGGGGAG 6595 0.0 241.54665 2 GGGAGCA 2140 0.0 199.8832 4 GCAAAAG 2380 0.0 172.28992 8 >>END_MODULE