##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_plasmids_flu.3510000003bb92.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462812
Sequences flagged as poor quality	0
Sequence length	301
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.787980864800396	34.0	34.0	34.0	34.0	34.0
2	33.87158284573434	34.0	34.0	34.0	34.0	34.0
3	33.88501162459055	34.0	34.0	34.0	34.0	34.0
4	33.895983682359144	34.0	34.0	34.0	34.0	34.0
5	33.90210495838483	34.0	34.0	34.0	34.0	34.0
6	37.66038909967762	38.0	38.0	38.0	38.0	38.0
7	37.632161655272554	38.0	38.0	38.0	38.0	38.0
8	37.64307537401796	38.0	38.0	38.0	38.0	38.0
9	37.55858534350881	38.0	38.0	38.0	37.0	38.0
10-14	37.64181049756705	38.0	38.0	38.0	37.8	38.0
15-19	37.61273389626889	38.0	38.0	38.0	37.2	38.0
20-24	37.61071493392565	38.0	38.0	38.0	37.4	38.0
25-29	37.51092106514092	38.0	38.0	38.0	37.0	38.0
30-34	37.468760965575655	38.0	38.0	38.0	37.0	38.0
35-39	37.410323846399834	38.0	38.0	38.0	37.0	38.0
40-44	37.405880573537424	38.0	38.0	38.0	37.0	38.0
45-49	37.382383343560676	38.0	38.0	38.0	37.0	38.0
50-54	37.313319447205345	38.0	38.0	38.0	37.0	38.0
55-59	37.245500980959875	38.0	38.0	38.0	37.0	38.0
60-64	37.03057008029178	38.0	38.0	38.0	36.0	38.0
65-69	36.94969620493851	38.0	38.0	38.0	35.6	38.0
70-74	37.0127546390327	38.0	38.0	38.0	36.0	38.0
75-79	36.95706075036948	38.0	38.0	38.0	36.0	38.0
80-84	36.82337277339395	38.0	38.0	38.0	35.8	38.0
85-89	36.8315933899726	38.0	38.0	38.0	35.6	38.0
90-94	36.606950554436786	38.0	38.0	38.0	34.6	38.0
95-99	36.53335004278195	38.0	37.6	38.0	34.4	38.0
100-104	36.52850703957547	38.0	38.0	38.0	34.0	38.0
105-109	36.35975514895897	38.0	37.2	38.0	34.0	38.0
110-114	36.23623328695021	38.0	37.0	38.0	34.0	38.0
115-119	36.246928342393886	38.0	37.0	38.0	34.0	38.0
120-124	36.01739151102392	38.0	37.0	38.0	33.2	38.0
125-129	35.87854679653942	38.0	37.0	38.0	32.0	38.0
130-134	35.64425727941367	38.0	37.0	38.0	30.2	38.0
135-139	35.32597426168725	38.0	36.4	38.0	27.8	38.0
140-144	35.10437196961185	38.0	36.0	38.0	26.6	38.0
145-149	34.45226528266337	38.0	35.0	38.0	23.8	38.0
150-154	34.008645843236565	38.0	34.6	38.0	22.2	38.0
155-159	33.98133713041148	37.8	34.2	38.0	23.0	38.0
160-164	33.86644469028461	37.8	34.0	38.0	23.0	38.0
165-169	33.671262197177256	37.2	34.0	38.0	22.2	38.0
170-174	33.36646024735746	37.0	34.0	38.0	20.8	38.0
175-179	32.6474953112711	37.0	31.4	38.0	18.4	38.0
180-184	32.051124862795255	37.0	29.0	38.0	17.2	38.0
185-189	31.302262257676983	36.6	27.2	37.6	15.6	38.0
190-194	29.96494300061364	35.0	24.0	37.0	12.8	37.8
195-199	29.45590779841491	34.4	23.0	37.0	11.4	37.6
200-204	29.227478112062784	34.2	22.6	37.0	11.0	37.4
205-209	28.0807571973069	32.4	20.8	37.0	9.2	37.2
210-214	27.178422772097527	30.6	19.6	37.0	9.0	37.0
215-219	26.22637053490402	29.0	18.6	36.2	8.4	37.0
220-224	24.33377440515803	25.8	15.2	34.6	8.0	37.0
225-229	23.46962524740067	24.4	13.8	33.2	8.0	37.0
230-234	23.177645350595924	24.2	13.2	33.0	7.8	37.0
235-239	22.341571955783344	22.8	12.2	32.4	7.0	36.6
240-244	21.32494879130187	20.8	11.4	30.8	7.0	36.0
245-249	20.443767231618885	19.2	11.0	29.2	7.0	36.0
250-254	19.739308401683623	19.0	9.0	29.0	7.0	35.6
255-259	19.081672039618677	18.2	8.4	27.8	7.0	34.2
260-264	18.120817092037374	17.0	8.2	25.8	7.0	33.4
265-269	17.381697103791602	15.6	8.0	24.8	7.0	31.8
270-274	16.601120109245223	14.0	8.0	23.0	7.0	30.8
275-279	15.609621185276097	13.0	8.0	21.0	7.0	29.8
280-284	15.291207228853185	13.0	8.0	21.0	7.0	28.8
285-289	14.432002627416747	12.0	7.8	19.2	7.0	26.4
290-294	13.994102572966995	11.8	7.2	18.8	7.0	25.8
295-299	13.445305221126507	11.0	7.2	18.0	7.0	25.0
300-301	12.144922776418934	9.5	7.0	15.5	7.0	21.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.011094578506600783
1101	2	0.026104105583080184
1101	3	0.006071958796191268
1101	4	-0.03290304550095868
1101	5	-0.03630841883347102
1101	6	0.12587155452908405
1101	7	0.10322528023766608
1101	8	0.09020343607685533
1101	9	0.16154277991653743
1101	10-14	0.05267029638599041
1101	15-19	0.08186859425739357
1101	20-24	0.07295034389216681
1101	25-29	0.12310857768630257
1101	30-34	0.1606470513278353
1101	35-39	0.1907494258628759
1101	40-44	0.13499836282718292
1101	45-49	0.15604753434584495
1101	50-54	0.18873795740969257
1101	55-59	0.14296344993557142
1101	60-64	0.3946632122615128
1101	65-69	0.4290778641263344
1101	70-74	0.36826491881441825
1101	75-79	0.25266376454447936
1101	80-84	0.34403782520509907
1101	85-89	0.2720836512201785
1101	90-94	0.3818078460220633
1101	95-99	0.27628342503150094
1101	100-104	0.4006922686616079
1101	105-109	0.41067560651408286
1101	110-114	0.29857680106339046
1101	115-119	0.2971825545008926
1101	120-124	0.3948103175454136
1101	125-129	0.3885408419298244
1101	130-134	0.32570861457354994
1101	135-139	0.36364213848234783
1101	140-144	0.18044831488300161
1101	145-149	0.6614724069499403
1101	150-154	0.8034884330814336
1101	155-159	0.44618091158852025
1101	160-164	0.28454875957179127
1101	165-169	0.14857474044018204
1101	170-174	0.42037214118056454
1101	175-179	0.5163601903144226
1101	180-184	-0.02773276360844079
1101	185-189	0.212564230195472
1101	190-194	0.6851927535698721
1101	195-199	0.15250807504567732
1101	200-204	-0.2626781571433874
1101	205-209	-0.08654451042510303
1101	210-214	-0.5464939711483296
1101	215-219	-0.10883421606411048
1101	220-224	0.3699218005746232
1101	225-229	0.7765516973525983
1101	230-234	0.3042708357948918
1101	235-239	0.41285487340779525
1101	240-244	0.4033381347434002
1101	245-249	0.6517817397145507
1101	250-254	0.08087466624486694
1101	255-259	-0.14813779565914587
1101	260-264	0.34204175579567675
1101	265-269	-0.04575856478867024
1101	270-274	0.14885648926839323
1101	275-279	0.3486316344848106
1101	280-284	-0.08366457837156815
1101	285-289	-8.789997905971347E-4
1101	290-294	-0.09641393617679839
1101	295-299	0.06677451083298358
1101	300-301	0.10706786845015515
1102	1	-0.003745312483012242
1102	2	0.02758383646368401
1102	3	0.06015665475939613
1102	4	0.019164319513521377
1102	5	0.03874534117334605
1102	6	0.07533714812472425
1102	7	0.12342520358973985
1102	8	0.01169253503785228
1102	9	0.07702246821494896
1102	10-14	0.07183866633999969
1102	15-19	0.036056552015857335
1102	20-24	0.0319261359207772
1102	25-29	0.07435942932996653
1102	30-34	0.13306657100079633
1102	35-39	0.04364075613028717
1102	40-44	0.03377731871204048
1102	45-49	0.10998638633017066
1102	50-54	0.11831075165257943
1102	55-59	0.05293460050616261
1102	60-64	0.27085711023460135
1102	65-69	0.3030601499379486
1102	70-74	0.24310417107127336
1102	75-79	0.08186130329426078
1102	80-84	0.20369759355802586
1102	85-89	0.2635757309340221
1102	90-94	0.381925479304293
1102	95-99	0.3030000193084561
1102	100-104	0.25639934582052604
1102	105-109	0.27701758403068766
1102	110-114	0.38930399830407225
1102	115-119	0.28326510006383643
1102	120-124	0.359833311924568
1102	125-129	0.32752589558336354
1102	130-134	0.4718889927033416
1102	135-139	0.5041063907391958
1102	140-144	0.5712729188686936
1102	145-149	0.654888136808566
1102	150-154	1.1773584083837605
1102	155-159	0.7205416465570096
1102	160-164	0.6453518595547649
1102	165-169	0.8994844023376416
1102	170-174	0.8864804489645906
1102	175-179	0.7960921354685624
1102	180-184	0.7677077490810547
1102	185-189	1.0683080344877531
1102	190-194	1.309567327748038
1102	195-199	0.7317790680621954
1102	200-204	0.3793307638240826
1102	205-209	0.26524926405965843
1102	210-214	-0.07090207879608812
1102	215-219	-0.011068716149267743
1102	220-224	0.9982400172327708
1102	225-229	1.3324168608681894
1102	230-234	0.8867205888182284
1102	235-239	0.9712877425953295
1102	240-244	0.9788001514356175
1102	245-249	1.0065573316069063
1102	250-254	0.6175959445620158
1102	255-259	0.4386936640581105
1102	260-264	0.8341480835081612
1102	265-269	0.8906657027977722
1102	270-274	0.8549473383571389
1102	275-279	0.8838934084585812
1102	280-284	0.6068140453722535
1102	285-289	0.8356389485997973
1102	290-294	0.4670175490897659
1102	295-299	0.5711934769375677
1102	300-301	0.22345029904119507
1103	1	0.01661198319521162
1103	2	0.030445411132056677
1103	3	0.040486671875356706
1103	4	0.031482462431789315
1103	5	0.04920346055716607
1103	6	0.04998948017436078
1103	7	0.1701918916513563
1103	8	0.16285321111829631
1103	9	0.16338586145414524
1103	10-14	0.15471307797885459
1103	15-19	0.08894807961534212
1103	20-24	0.10421405847105092
1103	25-29	0.12518687905183157
1103	30-34	0.15924757228449948
1103	35-39	-0.35479955195700796
1103	40-44	-0.19377028505347482
1103	45-49	-0.15351596711440862
1103	50-54	0.11550881540953384
1103	55-59	-0.27450201695211973
1103	60-64	0.2839441341969433
1103	65-69	0.3684345615768052
1103	70-74	0.14844488566263436
1103	75-79	0.2915997500208434
1103	80-84	0.2557482995913958
1103	85-89	0.29904000136620823
1103	90-94	0.49514354734023414
1103	95-99	0.3616705905536435
1103	100-104	0.32597840016370583
1103	105-109	0.1378150808214471
1103	110-114	0.29886727387103207
1103	115-119	0.13507110134752764
1103	120-124	0.43173746254502277
1103	125-129	0.35914388873698044
1103	130-134	0.478510729973344
1103	135-139	0.594391211021609
1103	140-144	0.6776619860488324
1103	145-149	0.847974893334019
1103	150-154	1.037559788358088
1103	155-159	-0.12145583953626726
1103	160-164	-0.8795967925633335
1103	165-169	0.7257876222777355
1103	170-174	1.0700927189688727
1103	175-179	0.810527843641438
1103	180-184	0.7115978860087324
1103	185-189	0.8479750755660582
1103	190-194	0.4902918142692201
1103	195-199	0.6116934882590321
1103	200-204	0.10480546429477045
1103	205-209	1.0992720496822486
1103	210-214	0.8650799494452492
1103	215-219	0.33108842976429287
1103	220-224	1.1465482114989207
1103	225-229	1.881982275930234
1103	230-234	0.9387242259598629
1103	235-239	1.1032258577001635
1103	240-244	0.8095564629460981
1103	245-249	0.6969542079440885
1103	250-254	0.6056193185457559
1103	255-259	0.42958262426349947
1103	260-264	1.222790039006668
1103	265-269	0.5154996244922501
1103	270-274	0.6615409947542261
1103	275-279	1.1678052077739434
1103	280-284	0.40401558580871466
1103	285-289	0.40793542913039005
1103	290-294	0.6432932230307156
1103	295-299	0.6533859245199363
1103	300-301	-0.7821952935989405
2116	1	-0.004136036484020167
2116	2	0.05427450977617809
2116	3	0.015796280509164262
2116	4	0.03077515840850964
2116	5	0.019363582704137627
2116	6	-0.23430106257055172
2116	7	-0.17468928910266612
2116	8	0.09560843433666122
2116	9	0.15342749060238248
2116	10-14	0.13095731971501579
2116	15-19	0.1190706180798955
2116	20-24	-0.15526577292437338
2116	25-29	-0.18207642857321105
2116	30-34	0.05107155537184127
2116	35-39	-0.09621520399474548
2116	40-44	-0.09610725930603792
2116	45-49	0.030048479766008995
2116	50-54	0.267967284135473
2116	55-59	0.17822632349341205
2116	60-64	0.35403248195040504
2116	65-69	0.38438987875437647
2116	70-74	-0.7855892267491242
2116	75-79	0.18448390614972254
2116	80-84	-0.11460835389488722
2116	85-89	0.2265562158541954
2116	90-94	0.3522159350785614
2116	95-99	0.3139690676315112
2116	100-104	0.3881963594219471
2116	105-109	0.387693325350277
2116	110-114	0.5627323902107975
2116	115-119	0.4827859351571817
2116	120-124	-0.08049785306656077
2116	125-129	-0.06443099805522223
2116	130-134	0.39314226633685934
2116	135-139	0.6913289238979345
2116	140-144	0.5516992304341173
2116	145-149	0.8365953894908316
2116	150-154	1.2295615109435332
2116	155-159	-0.35655691093221265
2116	160-164	-0.8220068461983843
2116	165-169	0.8027054993216822
2116	170-174	1.1494300455684652
2116	175-179	0.804821648858784
2116	180-184	0.8298692976153035
2116	185-189	1.1411418923064538
2116	190-194	1.0894305215471434
2116	195-199	1.0438429355961993
2116	200-204	1.1409001542946378
2116	205-209	1.114747130917813
2116	210-214	1.0849186528082058
2116	215-219	1.23800252234021
2116	220-224	1.262845174789586
2116	225-229	1.0458406063273387
2116	230-234	1.0573772163420898
2116	235-239	1.3790449340289648
2116	240-244	1.7873823513173406
2116	245-249	1.1344434091603368
2116	250-254	1.1165676540323233
2116	255-259	0.6544356172557109
2116	260-264	0.05295616410290904
2116	265-269	0.5456973303216373
2116	270-274	0.5849694617836789
2116	275-279	1.1405745248732106
2116	280-284	0.47250636606161933
2116	285-289	-0.5193404063888014
2116	290-294	0.252489480322339
2116	295-299	0.7130933459922257
2116	300-301	1.7133997161232255
1117	1	-0.06673635751131002
1117	2	0.05828735086767267
1117	3	0.02943994022024299
1117	4	0.01873663513403301
1117	5	0.05628172074587212
1117	6	-0.39401213801196633
1117	7	-0.31674386374151453
1117	8	-0.05045898139367466
1117	9	0.03625253073079193
1117	10-14	0.11426390077439663
1117	15-19	0.07733707072837603
1117	20-24	-2.0959314909418936E-4
1117	25-29	0.08582084269483659
1117	30-34	0.06471521508292
1117	35-39	-0.2727802120204288
1117	40-44	-0.12628382431406493
1117	45-49	0.059904017486722694
1117	50-54	0.04950821511460646
1117	55-59	0.2517415722895606
1117	60-64	0.24327806782520867
1117	65-69	0.3038120296372
1117	70-74	-0.14257157024832168
1117	75-79	0.21032660277893456
1117	80-84	-0.008187808148491627
1117	85-89	0.18434112757168464
1117	90-94	0.25943904904325166
1117	95-99	0.2759273340841588
1117	100-104	0.20906313309770752
1117	105-109	0.2409838550453003
1117	110-114	0.32854298411128724
1117	115-119	0.38679877624867487
1117	120-124	0.45208641659636584
1117	125-129	0.37601844014702834
1117	130-134	0.2099961989211323
1117	135-139	0.3786483460488199
1117	140-144	0.44399457553845423
1117	145-149	0.9280881663768668
1117	150-154	0.9358215430462593
1117	155-159	-0.055112288139277155
1117	160-164	-0.8929538766959695
1117	165-169	0.36963968872777997
1117	170-174	0.8934107839312162
1117	175-179	0.9738425156324624
1117	180-184	0.8411052526714826
1117	185-189	1.4281402871700202
1117	190-194	1.2993823674540472
1117	195-199	0.9539552951467591
1117	200-204	1.0657797690618942
1117	205-209	1.4320826044330595
1117	210-214	0.8142926495979346
1117	215-219	0.703813116240692
1117	220-224	1.323679845736617
1117	225-229	0.7779433350592804
1117	230-234	1.295739977176762
1117	235-239	1.0902808890851468
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2108	190-194	-0.7878339880414345
2108	195-199	-1.543038745702983
2108	200-204	-0.6805452483216641
2108	205-209	-0.3362942582075483
2108	210-214	0.07488739488812257
2108	215-219	-0.9354867323436054
2108	220-224	-0.6007326548320044
2108	225-229	-0.07118384763384
2108	230-234	-0.3968927715056587
2108	235-239	-1.0006732897933333
2108	240-244	-0.5585176623295673
2108	245-249	-0.24290623114612941
2108	250-254	-0.39153614045822493
2108	255-259	-0.4989885221767061
2108	260-264	0.046835330471722614
2108	265-269	-0.8004754915342112
2108	270-274	-0.7715481069869927
2108	275-279	-0.241519040934131
2108	280-284	-0.26597947689494994
2108	285-289	-0.19647390565121725
2108	290-294	-0.2634530986835131
2108	295-299	0.057129711755377954
2108	300-301	-0.2988422110802613
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	10.0
13	33.0
14	111.0
15	299.0
16	667.0
17	1168.0
18	2047.0
19	2557.0
20	3206.0
21	4507.0
22	6427.0
23	9270.0
24	12250.0
25	15527.0
26	19841.0
27	27192.0
28	35402.0
29	41945.0
30	45389.0
31	48996.0
32	54581.0
33	58367.0
34	49654.0
35	21525.0
36	1840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.54288998556649	24.842268566934305	17.438182242465622	20.17665920503358
2	34.94939629914523	26.307658401251484	18.878291833401033	19.86465346620226
3	22.068356049540636	24.029195440049094	20.79462070992109	33.10782780048918
4	23.28094345004019	24.436920391001095	19.951729860072774	32.33040629888594
5	35.06996361373517	24.098121915594238	20.903952360785805	19.927962109884792
6	32.77464715694494	24.108493297494444	21.37476988496409	21.742089660596527
7	33.67630917089445	23.926129832415754	22.023845535552233	20.373715461137568
8	23.703577262473747	31.876658340751753	22.271894419332256	22.147869977442245
9	32.096618065218706	21.919699575637626	24.287615705729323	21.696066653414345
10-14	28.042617736791613	23.570607503694806	24.37425995868733	24.012514800826253
15-19	23.43305705124327	23.252508577997112	21.985428208430204	31.329006162329414
20-24	26.40294547245966	23.725011451734183	22.606501127887782	27.265541947918376
25-29	27.725123808371432	23.306958332973217	23.342480315981433	25.625437542673918
30-34	25.786410032583422	23.35566061381295	23.42869242802693	27.429236925576696
35-39	26.22619983924358	24.66772685237202	23.886588938921204	25.219484369463196
40-44	27.354779046351435	24.069730257642412	24.903459720145545	23.672030975860608
45-49	26.906519277806108	25.60309585749721	23.18807636794206	24.30230849675462
50-54	26.913347104223746	24.77230495319914	25.554350362566225	22.75999758001089
55-59	24.721269111431855	26.688677043810443	21.910192475562432	26.67986136919527
60-64	29.226727051156843	23.484784318470567	22.026395166936037	25.262093463436557
65-69	28.403239328280165	23.489062513504404	22.333085572543496	25.774612585671935
70-74	25.473410369653337	23.311236528007054	23.416030699290424	27.79932240304919
75-79	28.154887945861383	23.674883105883165	24.51198326750387	23.65824568075158
80-84	28.11746454283813	24.019601911791398	23.637330060586155	24.22560348478432
85-89	27.725383092918936	25.496443480290054	22.6393870513297	24.13878637546131
90-94	28.89224998487507	23.28444379143151	25.585983077361867	22.237323146331555
95-99	26.589889631210944	23.43102598895448	24.59063291358046	25.388451466254114
100-104	26.506356792823006	23.51801595464249	26.64974114759341	23.325886104941098
105-109	26.837722444534712	26.825320000345716	21.0593070188327	25.27765053628687
110-114	26.75475138933303	24.495129771916027	24.745944357536107	24.004174481214836
115-119	24.687129979343663	24.297079591713267	22.24453989957045	28.771250529372615
120-124	25.231800385469693	23.519701304201273	22.792278506175293	28.456219804153736
125-129	24.396428787499026	23.948341875318704	22.086808466504756	29.56842087067751
130-134	24.665004364623215	23.255144637563415	22.70913459460861	29.370716403204757
135-139	26.611367034562626	23.811223563779677	20.505820938091492	29.0715884635662
140-144	27.243718831836684	23.285783428260288	21.426453938100135	28.044043801802893
145-149	25.30025150601108	24.522311435312826	24.01865120178388	26.158785856892212
150-154	27.541377492372714	26.07002411346292	21.62415840557289	24.76443998859148
155-159	25.2692670025842	24.983276146685913	20.422979525163566	29.324477325566324
160-164	22.587400499554892	23.516805960087463	22.70472675730102	31.19106678305662
165-169	25.892154913874315	23.43305705124327	20.465372548680673	30.209415486201742
170-174	27.679144015280503	23.897176391277668	20.378036870262655	28.045642723179178
175-179	28.400603268713866	25.76316085149045	20.714977139745727	25.121258740049957
180-184	23.33634391502381	27.00063956855051	24.338521905222855	25.32449461120282
185-189	22.57633769219467	24.19457576726619	25.084742832942965	28.144343707596175
190-194	24.470411311720525	24.30459884359092	21.241454413455138	29.983535431233417
195-199	25.283700509061998	26.397889423783305	21.520531014753292	26.797879052401406
200-204	25.323673543469056	26.20199994814309	21.237176218421304	27.23715028996655
205-209	25.39847713542432	24.85419565611955	23.795925775476867	25.951401432979264
210-214	25.31161681201006	25.40202069090689	19.830773618661574	29.455588878421473
215-219	26.5407552094587	25.6052133479685	20.848076540798424	27.00595490177437
220-224	22.411087007251325	27.895041615169873	23.36443307433688	26.32943830324192
225-229	24.48203590226701	28.528214480177695	20.978367025919813	26.011382591635478
230-234	21.896363966362152	31.053170617875075	19.71552163729549	27.33494377846728
235-239	25.079125001080353	27.73731018210418	20.469693957805763	26.71387085900971
240-244	21.490065080421424	27.426903364649146	24.75843322990761	26.324598325021825
245-249	21.556874065495276	29.3035617054009	21.25541256492917	27.884151664174656
250-254	22.161655272551272	29.177981556225852	21.271488206874498	27.388874964348375
255-259	21.86313233019023	30.49627062392505	19.67814144836348	27.96245559752124
260-264	21.298497013906296	29.644261600822798	20.929535102806323	28.127706282464587
265-269	21.179312550236382	30.66039774249587	20.651106712876935	27.509182994390812
270-274	21.318159425425442	30.173807075011023	20.165293899034598	28.342739600528937
275-279	20.821067733766625	30.95421034891057	20.22674433679334	27.997977580529458
280-284	20.94025219743654	31.010042954806703	19.956353767836614	28.093351079920144
285-289	20.02424310519174	30.937529709687734	18.904868499520326	30.133358685600196
290-294	19.059877444837213	32.2174014502649	18.89968280857022	29.823038296327663
295-299	20.08988530980182	32.645437024104815	17.533555741856304	29.73112192423705
300-301	15.707349852639949	36.152152493885204	20.868624841188215	27.27187281228663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.25
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.25
28	0.25
29	0.0
30	1.5
31	3.0
32	6.25
33	12.0
34	28.0
35	104.0
36	349.75
37	1133.0
38	2357.75
39	3363.5
40	4771.25
41	6051.5
42	8673.0
43	13874.0
44	15321.25
45	13433.25
46	9547.5
47	6603.75
48	4317.75
49	2755.0
50	2160.75
51	1848.0
52	1573.5
53	1442.25
54	1439.5
55	1289.75
56	1151.75
57	1104.5
58	1149.75
59	1198.25
60	1219.0
61	1336.75
62	1700.25
63	2688.25
64	5010.0
65	5962.25
66	5099.25
67	4354.25
68	3502.5
69	2748.0
70	2105.75
71	1661.75
72	1533.75
73	1410.75
74	1288.5
75	1144.75
76	911.25
77	689.5
78	527.0
79	429.0
80	349.75
81	307.0
82	292.25
83	267.75
84	226.25
85	197.0
86	149.0
87	96.25
88	44.0
89	20.0
90	10.0
91	7.25
92	3.75
93	5.25
94	3.25
95	1.5
96	1.0
97	0.25
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	462812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.9349867874721305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.10747266206246	2.6771225426560106
2	4.6471432372735535	0.5516146742541801
3	4.320637001360499	0.769287705496133
4	4.24082060134614	1.0067685694811583
5	3.0823602948496642	0.9146883812080728
6	3.050920354689066	1.0864303196825635
7	2.5060890360306285	1.0411523721949383
8	2.278202322993863	1.0816880548925514
9	1.682423243905974	0.8986643751166161
>10	19.482747101749233	25.746718562007846
>50	7.187228834355454	30.125562568107068
>100	2.3774617341133837	20.578007889381663
>500	0.02189614516206361	0.7911992044141989
>1k	0.0072987150540056575	1.315777488860568
>5k	0.0	0.0
>10k+	0.0072987150540056575	11.415317292246295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG	39399	8.512959905966138	No Hit
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG	13306	2.8750334909207194	No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC	4285	0.9258619050499987	No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT	1790	0.386766116695332	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	750	0.1620528421907816	TruSeq Adapter, Index 1 (97% over 36bp)
ACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCT	667	0.14411899432166841	No Hit
GGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCC	611	0.1320190487714234	No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT	599	0.1294262032963709	No Hit
CCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAA	520	0.11235663725227521	No Hit
CTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATAC	506	0.10933165086471396	No Hit
GGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCCGGG	488	0.10544238265213521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	2.1607045625437544E-4	0.0	0.0
6	4.3214091250875087E-4	0.0	0.0
7	4.3214091250875087E-4	0.0	0.0
8	8.642818250175017E-4	0.0	0.0
9	8.642818250175017E-4	0.0	0.0
10-14	8.642818250175017E-4	0.0	0.0
15-19	0.001037138190021002	0.0	0.0
20-24	0.0022039186537946296	0.0	0.0
25-29	0.0027657018400560054	0.0	0.0
30-34	0.004278195033836633	0.0	0.0
35-39	0.005315333223857635	0.0	0.0
40-44	0.008599604158924143	0.0	0.0
45-49	0.01400136556528353	0.0	0.0
50-54	0.019316698789141162	0.0	0.0
55-59	0.026619880210539053	0.0	0.0
60-64	0.038849468034536705	0.0	0.0
65-69	0.05816616682367787	0.0	0.0
70-74	0.08331676793168716	0.0	0.0
75-79	0.11641876182985748	0.0	0.0
80-84	0.1578178612481958	0.0	0.0
85-89	0.2097179848404968	0.0	0.0
90-94	0.2782555335643847	0.0	0.0
95-99	0.3657640683474067	0.0	0.0
100-104	0.47224358918956294	0.0	0.0
105-109	0.6000708711096514	0.0	0.0
110-114	0.7586665860003631	0.0	0.0
115-119	0.9586614003094128	0.0	0.0
120-124	1.1844118130039842	0.0	0.0
125-129	1.4685012488872373	0.0	0.0
130-134	1.790316586432504	0.0	0.0
135-139	2.159710638444984	0.0	0.0
140-144	2.591937979136237	0.0	0.0
145-149	3.059946587383214	0.0	0.0
150-154	3.587331357008894	0.0	0.0
155-159	4.246605533132245	0.0	0.0
160-164	4.940062055435035	0.0	0.0
165-169	5.818647744656578	0.0	0.0
170-174	6.693257737483039	0.0	0.0
175-179	7.768510755987312	0.0	0.0
180-184	8.800765753696965	0.0	0.0
185-189	9.9160782347908	0.0	0.0
190-194	11.306102693966448	0.0	0.0
195-199	12.762849710033448	0.0	0.0
200-204	14.263804741450091	0.0	0.0
205-209	15.703741476020502	0.0	0.0
210-214	17.229458181723896	0.0	0.0
215-219	18.719825760784076	0.0	0.0
220-224	20.219354727189444	0.0	0.0
225-229	21.749003915196667	0.0	0.0
230-234	23.287684848275326	0.0	0.0
235-239	24.71202129590417	0.0	0.0
240-244	26.09081009135459	0.0	0.0
245-249	27.47322887047008	0.0	0.0
250-254	28.791172225439272	0.0	0.0
255-259	30.03936803712954	0.0	0.0
260-264	31.192838560797902	0.0	2.1607045625437544E-4
265-269	32.20322722833462	0.0	2.1607045625437544E-4
270-274	33.14352263986241	0.0	2.1607045625437544E-4
275-279	34.008063749427414	0.0	2.1607045625437544E-4
280-284	34.787084172406935	0.0	2.1607045625437544E-4
285-289	35.46057578455183	0.0	2.1607045625437544E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCGT	4380	0.0	288.26483	8
GGGCGTC	4390	0.0	286.93622	9
GGCGGTG	1710	0.0	240.6579	8
GAGGGCG	5350	0.0	236.8271	7
GGGCGGT	2035	0.0	202.9484	7
GGAGGGC	6325	0.0	199.85376	6
GCCCGTG	185	0.0	199.32433	5
CGCGCTC	605	0.0	187.72728	4
CGGGGGC	2265	0.0	181.03752	4
CGCCCGT	215	0.0	171.51164	4
CGGCCAT	180	0.0	163.8889	295
GGGAGGG	7910	0.0	160.36662	5
GGGGGCG	2625	0.0	157.33334	5
CGTGCCA	235	0.0	156.91489	8
CTCCCCT	720	0.0	155.69444	8
GGCCGCG	825	0.0	137.66666	1
CCGCGCT	835	0.0	134.2515	3
GCCGGGA	9505	0.0	132.99054	2
CGGGAGG	9835	0.0	128.52821	4
CCGGGAG	9955	0.0	127.12707	3
>>END_MODULE