FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_plasmids_flu.3510000003bb92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_plasmids_flu.3510000003bb92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462812
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG393998.512959905966138No Hit
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG133062.8750334909207194No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC42850.9258619050499987No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT17900.386766116695332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT7500.1620528421907816TruSeq Adapter, Index 1 (97% over 36bp)
ACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCT6670.14411899432166841No Hit
GGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCC6110.1320190487714234No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT5990.1294262032963709No Hit
CCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAA5200.11235663725227521No Hit
CTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATAC5060.10933165086471396No Hit
GGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCCGGG4880.10544238265213521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT43800.0288.264838
GGGCGTC43900.0286.936229
GGCGGTG17100.0240.65798
GAGGGCG53500.0236.82717
GGGCGGT20350.0202.94847
GGAGGGC63250.0199.853766
GCCCGTG1850.0199.324335
CGCGCTC6050.0187.727284
CGGGGGC22650.0181.037524
CGCCCGT2150.0171.511644
CGGCCAT1800.0163.8889295
GGGAGGG79100.0160.366625
GGGGGCG26250.0157.333345
CGTGCCA2350.0156.914898
CTCCCCT7200.0155.694448
GGCCGCG8250.0137.666661
CCGCGCT8350.0134.25153
GCCGGGA95050.0132.990542
CGGGAGG98350.0128.528214
CCGGGAG99550.0127.127073
Produced by FastQC (version 0.11.2)