##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_8561_flu.3510000003bac9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346804
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.811789944752654	34.0	34.0	34.0	34.0	34.0
2	33.88080298958489	34.0	34.0	34.0	34.0	34.0
3	33.88418530351438	34.0	34.0	34.0	34.0	34.0
4	33.89157564503293	34.0	34.0	34.0	34.0	34.0
5	33.87820209686163	34.0	34.0	34.0	34.0	34.0
6	37.64067888490329	38.0	38.0	38.0	38.0	38.0
7	37.6559814765689	38.0	38.0	38.0	38.0	38.0
8	37.653325797857	38.0	38.0	38.0	38.0	38.0
9	37.56744443547364	38.0	38.0	38.0	37.0	38.0
10-14	37.64604214484262	38.0	38.0	38.0	37.6	38.0
15-19	37.610932976551595	38.0	38.0	38.0	37.0	38.0
20-24	37.60495438345578	38.0	38.0	38.0	37.0	38.0
25-29	37.515543073320956	38.0	38.0	38.0	37.0	38.0
30-34	37.458300365624396	38.0	38.0	38.0	37.0	38.0
35-39	37.36162558678677	38.0	38.0	38.0	37.0	38.0
40-44	37.359732875053346	38.0	38.0	38.0	37.0	38.0
45-49	37.34225268451344	38.0	38.0	38.0	37.0	38.0
50-54	37.29626417227022	38.0	38.0	38.0	37.0	38.0
55-59	37.24029307620442	38.0	38.0	38.0	37.0	38.0
60-64	37.07083020957083	38.0	38.0	38.0	36.4	38.0
65-69	37.00166491735966	38.0	38.0	38.0	36.0	38.0
70-74	37.0700516718377	38.0	38.0	38.0	36.2	38.0
75-79	36.93940438979943	38.0	38.0	38.0	36.0	38.0
80-84	36.82082501931927	38.0	38.0	38.0	35.8	38.0
85-89	36.8450046712264	38.0	38.0	38.0	36.0	38.0
90-94	36.6971459383398	38.0	38.0	38.0	35.2	38.0
95-99	36.68338946494274	38.0	38.0	38.0	35.0	38.0
100-104	36.65740706566245	38.0	38.0	38.0	35.0	38.0
105-109	36.49546948708781	38.0	38.0	38.0	34.0	38.0
110-114	36.393794189225034	38.0	37.0	38.0	34.0	38.0
115-119	36.48192523730983	38.0	37.0	38.0	34.0	38.0
120-124	36.388723313456595	38.0	37.0	38.0	33.8	38.0
125-129	36.27185787937855	38.0	37.0	38.0	34.0	38.0
130-134	36.126753439983396	38.0	37.0	38.0	33.2	38.0
135-139	35.866420802528225	38.0	37.0	38.0	31.6	38.0
140-144	35.819353294656345	38.0	37.0	38.0	31.0	38.0
145-149	35.41010138291369	38.0	36.2	38.0	28.6	38.0
150-154	35.008381102870786	38.0	35.6	38.0	27.0	38.0
155-159	35.05427273041833	38.0	35.6	38.0	27.6	38.0
160-164	34.999103816564975	38.0	35.8	38.0	27.4	38.0
165-169	34.79757384574573	38.0	35.2	38.0	26.2	38.0
170-174	34.66039261369534	38.0	35.0	38.0	24.8	38.0
175-179	34.2553586463824	37.6	34.4	38.0	23.2	38.0
180-184	33.96567571308289	37.2	34.0	38.0	23.0	38.0
185-189	33.21313075973749	37.0	32.8	37.8	21.6	38.0
190-194	32.28632541723856	36.8	30.2	37.0	20.2	38.0
195-199	31.95829978892977	36.4	29.6	37.2	19.8	37.8
200-204	31.782927532554414	36.8	29.8	37.0	19.4	37.4
205-209	30.776189432647833	35.2	27.0	37.0	16.4	37.2
210-214	30.076751709899543	34.2	25.4	37.0	15.4	37.0
215-219	29.253689692160414	33.0	23.8	36.8	13.4	37.0
220-224	27.81469879240147	31.2	21.4	36.2	9.2	37.0
225-229	26.906986078591945	29.8	20.0	36.0	9.2	37.0
230-234	26.506631411402406	29.2	20.0	35.8	9.0	37.0
235-239	25.518145696128077	27.6	18.4	34.6	9.0	37.0
240-244	24.482191670222953	25.8	16.6	33.4	8.4	37.0
245-249	23.740206572011857	25.0	16.0	33.0	8.0	36.2
250-254	22.91744443547364	23.8	14.4	31.8	8.0	36.0
255-259	22.330123066631298	22.8	13.6	31.0	8.0	36.0
260-264	21.131447734166848	21.2	12.0	29.4	8.0	35.2
265-269	20.42886933253365	20.4	11.4	28.8	8.0	34.0
270-274	19.578379718803706	19.2	9.4	27.2	7.6	33.6
275-279	18.344263041948764	18.0	8.4	25.8	7.0	32.0
280-284	18.243199040380155	18.0	8.4	25.2	7.0	31.6
285-289	17.051028246502348	15.8	8.0	23.4	7.0	30.2
290-294	16.447576152524192	14.8	8.0	22.4	7.0	29.6
295-299	15.60920519947867	13.0	8.0	21.0	7.0	27.2
300-301	13.664278670372891	11.0	8.0	18.0	7.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.07113251526101294
1101	2	-0.009474988557656161
1101	3	0.03566109233145909
1101	4	3.8264257773334975E-4
1101	5	0.01577111967604594
1101	6	0.03240310520851608
1101	7	0.10199460441252484
1101	8	0.016221073991850687
1101	9	0.14268196707757852
1101	10-14	0.03778234212747833
1101	15-19	0.07055621192920114
1101	20-24	0.04835014000922655
1101	25-29	0.08694041532890395
1101	30-34	0.14939333204090133
1101	35-39	0.2313678380135329
1101	40-44	0.14593628558586857
1101	45-49	0.16150760517111706
1101	50-54	0.10120500152321199
1101	55-59	0.165579370996916
1101	60-64	0.41763713463980423
1101	65-69	0.29665757157015094
1101	70-74	0.24050718922459424
1101	75-79	0.1414946842750595
1101	80-84	0.3194092700608593
1101	85-89	0.36301218577576577
1101	90-94	0.4396039824547131
1101	95-99	0.29204853768759875
1101	100-104	0.2819163150416344
1101	105-109	0.2979796618433639
1101	110-114	0.4000705181961166
1101	115-119	0.14812925313444225
1101	120-124	0.22736820954956016
1101	125-129	0.34498246102412367
1101	130-134	0.2498469512907917
1101	135-139	0.34899497081058684
1101	140-144	0.16349959168842076
1101	145-149	0.5262468828615496
1101	150-154	0.712022637197208
1101	155-159	0.4272128485021014
1101	160-164	0.17697811397898278
1101	165-169	0.20002309318815747
1101	170-174	0.29444408816441836
1101	175-179	0.4139412505831146
1101	180-184	-0.03976967122397923
1101	185-189	0.16724475347351841
1101	190-194	0.31464404944907187
1101	195-199	0.1197100418277266
1101	200-204	-0.2718906688114089
1101	205-209	-0.36399403781795314
1101	210-214	-0.37273407303489847
1101	215-219	-0.51325376440467
1101	220-224	0.24214621060831476
1101	225-229	0.7777053715815683
1101	230-234	0.12986650289608548
1101	235-239	0.041159198876886904
1101	240-244	0.47135839593676465
1101	245-249	0.35599846798726276
1101	250-254	0.03203302878581127
1101	255-259	-0.2445655400750688
1101	260-264	0.3787550635892245
1101	265-269	0.07051559355182135
1101	270-274	-0.023202972368583374
1101	275-279	0.13059063075003152
1101	280-284	0.04253984621884044
1101	285-289	0.11919610061540453
1101	290-294	-0.06825346992588877
1101	295-299	0.09181199299715637
1101	300-301	0.2729869093497044
1102	1	-0.01581057816196818
1102	2	-0.0018201810158018361
1102	3	0.03862913332660867
1102	4	0.03738780608892256
1102	5	0.020514117641937446
1102	6	-0.06766437249450519
1102	7	0.123730492771152
1102	8	0.013869133763400043
1102	9	0.09226194282472022
1102	10-14	0.08165482882752428
1102	15-19	0.07182386370514138
1102	20-24	0.0724942000154769
1102	25-29	0.08383913031873647
1102	30-34	0.07927666407032063
1102	35-39	0.046299969135425556
1102	40-44	-0.007099966565597526
1102	45-49	0.045143773219926686
1102	50-54	-0.011590091185276208
1102	55-59	0.05305736739810385
1102	60-64	0.12703071545657707
1102	65-69	0.2784818654991099
1102	70-74	0.09970078177661179
1102	75-79	0.03701157325956217
1102	80-84	0.20673152987935595
1102	85-89	0.10129282456468758
1102	90-94	0.3020152346061806
1102	95-99	0.09845451875472122
1102	100-104	0.16725751751386042
1102	105-109	0.04568273061194361
1102	110-114	0.22333516770323314
1102	115-119	0.02440326150558292
1102	120-124	0.12964900480852037
1102	125-129	0.0696083998443342
1102	130-134	0.1825310969643894
1102	135-139	0.1674207844547695
1102	140-144	0.24335138522621236
1102	145-149	0.3297574348096006
1102	150-154	0.7269936413436682
1102	155-159	0.6683806039394327
1102	160-164	0.6432221485588556
1102	165-169	0.551850858796044
1102	170-174	0.5732949624407482
1102	175-179	0.7275705241709645
1102	180-184	0.6424702462380765
1102	185-189	0.7634481547409209
1102	190-194	1.0009644120683845
1102	195-199	0.8357257279488444
1102	200-204	0.5337011790997153
1102	205-209	0.4625303226107711
1102	210-214	0.2213088193137196
1102	215-219	-0.1375980571601083
1102	220-224	0.7634691647626006
1102	225-229	0.9954006657452332
1102	230-234	0.8339276337832686
1102	235-239	1.479910959164794
1102	240-244	1.1773238160588129
1102	245-249	1.1988076918958797
1102	250-254	0.4982792637255713
1102	255-259	0.2983717936755177
1102	260-264	1.2770187351586202
1102	265-269	0.9899276573916005
1102	270-274	1.1140606111864173
1102	275-279	1.2570088849356047
1102	280-284	0.6697939926747942
1102	285-289	1.2404410642361228
1102	290-294	0.6141683439530006
1102	295-299	0.7238000914950433
1102	300-301	0.3138286447979901
1103	1	-0.03291563832858202
1103	2	0.009571943328531063
1103	3	0.0032908889575864464
1103	4	0.01810363453692787
1103	5	0.033415228410220266
1103	6	0.014399419544986358
1103	7	0.13380888292851267
1103	8	0.01802487273285891
1103	9	0.08432168518818628
1103	10-14	0.07531081849120369
1103	15-19	0.017782383452129125
1103	20-24	0.019533597577968465
1103	25-29	0.021429283820737055
1103	30-34	0.13548905993579297
1103	35-39	-0.46958054613764943
1103	40-44	-0.2913038195430744
1103	45-49	-0.18403896358969973
1103	50-54	0.06168252914795147
1103	55-59	-0.32862363383607374
1103	60-64	0.17122521561085335
1103	65-69	0.24006442103753045
1103	70-74	-0.008628051916687696
1103	75-79	0.3145488881315259
1103	80-84	0.32053176514624937
1103	85-89	0.2412809647991807
1103	90-94	0.4576216253806322
1103	95-99	0.31759695164581103
1103	100-104	0.2274598084734336
1103	105-109	0.09425425560423406
1103	110-114	0.31521556727125954
1103	115-119	0.13678128912051335
1103	120-124	0.4082749111645896
1103	125-129	0.39947090370117166
1103	130-134	0.4158801790378206
1103	135-139	0.39199942530636633
1103	140-144	0.4015688528288024
1103	145-149	0.6793982671144434
1103	150-154	0.939529799165328
1103	155-159	-0.1251389502105269
1103	160-164	-0.6707039159274117
1103	165-169	0.41560625836407894
1103	170-174	0.7492239774052649
1103	175-179	0.530765429292849
1103	180-184	0.5130488678055016
1103	185-189	0.7937739608192871
1103	190-194	0.37338756464474443
1103	195-199	0.693287051614373
1103	200-204	0.4899608211854911
1103	205-209	0.9418784211914577
1103	210-214	0.6769793299585842
1103	215-219	0.422203123031192
1103	220-224	1.1267752431334692
1103	225-229	1.925697063461989
1103	230-234	1.1712015268718226
1103	235-239	1.2378444673412794
1103	240-244	1.2367576327820444
1103	245-249	1.3871735511985683
1103	250-254	1.5400347982828073
1103	255-259	0.7219486218149775
1103	260-264	1.8324346947389962
1103	265-269	0.6461024491225444
1103	270-274	0.993666153457319
1103	275-279	1.7182211843802229
1103	280-284	0.7507817279416855
1103	285-289	0.6459105373925524
1103	290-294	1.0976943583004228
1103	295-299	1.137803774771811
1103	300-301	-0.9634331474729141
2116	1	-0.01727646302337149
2116	2	-0.012514477391071921
2116	3	0.004713888124044274
2116	4	0.06830465043859846
2116	5	0.022779576064110074
2116	6	-0.20499113155634063
2116	7	-0.09773472577894893
2116	8	0.06524173504271857
2116	9	0.05378246521278385
2116	10-14	0.12303609215374678
2116	15-19	0.11461770698871732
2116	20-24	-0.1065869567009301
2116	25-29	-0.1654445850330788
2116	30-34	0.06946189861189112
2116	35-39	-0.06285514813536253
2116	40-44	-0.16245377786624005
2116	45-49	0.07508780307068008
2116	50-54	0.253431897087637
2116	55-59	0.2104867384002418
2116	60-64	0.4576210672689456
2116	65-69	0.36325332072298977
2116	70-74	-0.8120662757926809
2116	75-79	0.2465631457542372
2116	80-84	-0.10873688696499073
2116	85-89	0.1608244077203338
2116	90-94	0.2746464979324088
2116	95-99	0.2599217362387378
2116	100-104	0.2634262275351702
2116	105-109	0.3016828638742055
2116	110-114	0.547209101605155
2116	115-119	0.3907645354927922
2116	120-124	-0.18987058474372276
2116	125-129	0.018855468074619353
2116	130-134	0.2840169711648102
2116	135-139	0.5549940786978524
2116	140-144	0.6130242263775969
2116	145-149	0.6560200947243118
2116	150-154	0.9994583268447315
2116	155-159	-0.2635157180221839
2116	160-164	-0.6626347277737707
2116	165-169	0.7151904110348468
2116	170-174	0.9910288377072689
2116	175-179	0.7088305188404789
2116	180-184	0.6688861577519418
2116	185-189	1.0050626270223688
2116	190-194	0.9043859205971643
2116	195-199	0.9204405467121681
2116	200-204	0.7055295373086636
2116	205-209	0.7927508716512861
2116	210-214	0.4924508768830158
2116	215-219	1.08245124987614
2116	220-224	1.3565928321399987
2116	225-229	0.946313512227114
2116	230-234	0.9372558587299622
2116	235-239	0.9023256713183123
2116	240-244	1.1298291465492198
2116	245-249	0.8986344509577151
2116	250-254	0.804219428970768
2116	255-259	0.9456265911168806
2116	260-264	0.08030364987077121
2116	265-269	0.5293999388414896
2116	270-274	0.8776433670499557
2116	275-279	1.5010553934643909
2116	280-284	0.04645977092638631
2116	285-289	-1.3562640263822168
2116	290-294	0.09327615312144388
2116	295-299	0.32255742091935247
2116	300-301	1.0795619203843447
1117	1	0.005016177954843215
1117	2	0.06123477162823576
1117	3	0.02170431091145275
1117	4	0.03478322834622105
1117	5	0.04138033705788047
1117	6	-0.36943528804304293
1117	7	-0.10146312404020819
1117	8	0.028514950653693916
1117	9	0.1422002814198251
1117	10-14	0.1006798386933454
1117	15-19	0.08263853418658584
1117	20-24	0.06282297862637165
1117	25-29	0.12419553323902477
1117	30-34	0.11469744149731298
1117	35-39	-0.09614379566331621
1117	40-44	-0.12523458574476365
1117	45-49	0.09623000052852859
1117	50-54	0.15322221149822468
1117	55-59	0.2240286578479882
1117	60-64	0.3405967178243756
1117	65-69	0.2703798035417293
1117	70-74	-0.06283374861012447
1117	75-79	0.1815639114600458
1117	80-84	-0.0010765304994890812
1117	85-89	0.14351238849523185
1117	90-94	0.2009405996416973
1117	95-99	0.2889492898675883
1117	100-104	0.2126102207498448
1117	105-109	0.23232711695764863
1117	110-114	0.29856652129439
1117	115-119	0.38157300302097497
1117	120-124	0.4504912995993635
1117	125-129	0.2645871883210589
1117	130-134	0.294558030666046
1117	135-139	0.4966892335471229
1117	140-144	0.43996222776764426
1117	145-149	0.8114703886375381
1117	150-154	1.011217447578872
1117	155-159	-0.05453622715765505
1117	160-164	-0.42080952073869327
1117	165-169	0.4280179858678679
1117	170-174	0.8130663219514034
1117	175-179	0.8349158773675001
1117	180-184	0.9120467557092695
1117	185-189	1.1110332474796678
1117	190-194	1.3109745245387785
1117	195-199	0.9930412370253983
1117	200-204	0.8173574537642736
1117	205-209	1.286621926793881
1117	210-214	0.8864859108685721
1117	215-219	0.8509906366046884
1117	220-224	1.3132442239575433
1117	225-229	0.7787999356742041
1117	230-234	1.34072839345178
1117	235-239	0.8744235874205124
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2108	180-184	-0.5292468704866593
2108	185-189	-0.9564373266255402
2108	190-194	-0.5692865442261237
2108	195-199	-1.693458297198866
2108	200-204	-1.0417059561828168
2108	205-209	-0.5316800716476706
2108	210-214	-0.01984735020604944
2108	215-219	-0.8169170050467933
2108	220-224	-0.5143107170734922
2108	225-229	-0.28466438121734683
2108	230-234	-0.40880003058576264
2108	235-239	-1.1912809057041684
2108	240-244	-0.2781488395994174
2108	245-249	0.03692218212463416
2108	250-254	-0.40269336352787377
2108	255-259	-0.9432715179439377
2108	260-264	-0.08480475749400895
2108	265-269	-0.9292720850716663
2108	270-274	-1.0937940225811964
2108	275-279	-0.6031578985062964
2108	280-284	-0.825527738881032
2108	285-289	-0.2673340361274583
2108	290-294	-0.5291042189162631
2108	295-299	-0.08196631700367085
2108	300-301	-0.4767683054746943
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	19.0
14	80.0
15	202.0
16	328.0
17	568.0
18	1584.0
19	1309.0
20	1134.0
21	1340.0
22	1961.0
23	2923.0
24	4176.0
25	5966.0
26	8135.0
27	10749.0
28	14961.0
29	20284.0
30	28128.0
31	39860.0
32	54346.0
33	65740.0
34	56985.0
35	23909.0
36	2110.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.779345105592785	21.28003137218717	15.977036020345786	31.963587501874258
2	43.849551908282486	24.207909943368588	17.05199478668066	14.890543361668263
3	43.99660903565126	22.184000184542278	19.512750717984797	14.306640061821662
4	44.376939135650105	22.071833081510018	18.623775965675136	14.927451817164739
5	28.678446615379293	22.162662483708377	34.73720026297274	14.421690637939585
6	27.10032179559636	22.790394574457043	34.964129594814366	15.145154035132236
7	16.83832943103309	47.74368231046932	20.54618747188614	14.871800786611459
8	26.198371414401215	21.493696727834745	36.34127634052663	15.966655517237403
9	15.82651872527422	19.3181162847026	37.90613718411552	26.94922780590766
10-14	26.138452843681158	34.36021499175327	23.32499048453882	16.17634168002676
15-19	21.316651480375082	35.625367642818425	21.681929850866773	21.376051025939724
20-24	24.854845964867764	31.10725366489429	26.542254414597295	17.49564595564065
25-29	19.034670880382002	25.69128383755666	37.71571262153839	17.558332660522947
30-34	19.12815307781917	28.654975144462004	31.19710268624353	21.0197690914753
35-39	20.697569808883404	30.17692990853623	27.52476903380584	21.600731248774522
40-44	20.00369084554965	29.444008719622612	29.184150125142732	21.36815030968501
45-49	20.790186964394874	30.80327793220378	26.425300746242836	21.98123435715851
50-54	21.79957555276179	29.780625367642816	27.549912919112813	20.86988616048258
55-59	21.363594422209665	26.907013759933562	29.856864396027728	21.872527421829044
60-64	21.42916460017762	26.617916748365072	28.823773658896666	23.12914499256064
65-69	20.229870474388992	27.552277367043054	29.30675540074509	22.911096757822865
70-74	20.80183619566095	26.672587398069226	29.19608770371737	23.32948870255245
75-79	20.427042363986576	26.176918374643893	30.73730406800383	22.658735193365708
80-84	23.442001822354992	25.504838467837743	27.441148314321634	23.612011395485634
85-89	20.65333733174935	29.072732725112743	26.36117230481771	23.912757638320205
90-94	20.120932861212673	30.826576394735934	28.61368380987532	20.438806934176075
95-99	21.708284794869726	30.65316432336421	26.525241923391885	21.113308958374184
100-104	22.155165453685655	29.20035524388415	26.395946990230794	22.248532312199398
105-109	23.33035374447815	27.95036966124958	24.149144761882795	24.570131832389478
110-114	21.21844038707743	28.528275337078	28.94326478356651	21.31001949227806
115-119	23.034970761582912	28.530582115546533	26.637524365347577	21.79692275752298
120-124	18.91800555933611	29.007739241761914	27.93629831259155	24.137956886310423
125-129	20.76786888271185	25.563603649323536	30.39342106780775	23.27510640015686
130-134	24.323940900335636	29.162178060229987	25.786207771536663	20.727673267897718
135-139	23.796034647812597	27.86928639808076	24.95634421748307	23.378334736623568
140-144	20.80137483996724	29.707615829113855	26.799229535991508	22.691779794927395
145-149	19.98252615310089	28.179375093712878	28.940842666174554	22.897256087011684
150-154	22.98122282326617	26.22190055478022	25.899470594341473	24.89740602761214
155-159	21.73625448380065	27.699968858490674	29.62710926056216	20.936667397146515
160-164	24.19349257794028	29.514769149144758	26.509901846576163	19.7818364263388
165-169	19.369615114012525	26.149410041406675	28.772390168510164	25.708584676070632
170-174	21.48441194449891	29.52970553972849	26.77345128660569	22.212431229166906
175-179	20.264875837648933	29.287436131071154	28.70434020368854	21.74334782759138
180-184	19.535818502670097	30.02174138706589	26.910070241404366	23.532369868859643
185-189	21.945709968743152	27.983183584964415	28.02355220816369	22.04755423812874
190-194	19.60571389026655	28.201462497549045	28.14015985974787	24.052663752436533
195-199	20.52150494227287	30.33206076054486	27.6931061925468	21.45332810463547
200-204	21.756900151093987	27.786011695366835	28.13964083459245	22.317447318946726
205-209	20.877498529428724	27.92972399395624	28.8900935398669	22.302683936748135
210-214	22.49691468379834	27.84546891039319	28.178106365555184	21.479510040253285
215-219	22.234806980311646	27.704986101659728	28.776773047600372	21.283433870428254
220-224	18.8099906575472	27.35360607144093	28.882192823612186	24.95421044739968
225-229	20.88488022052802	29.06540870347516	27.42615425427619	22.623556821720626
230-234	20.70108764604791	27.50983264322211	29.714190147749157	22.074889562980818
235-239	21.327204991868605	27.218601861570225	27.316524607559312	24.137668539001858
240-244	22.74345163262246	31.56693694421056	24.128729772436305	21.560881650730675
245-249	20.160436442486247	29.563903530524442	25.790302303318303	24.485357723671008
250-254	21.259789391125825	26.949516153216223	26.710764581723396	25.07992987393456
255-259	21.467572461678643	27.48013287043979	28.397077311680373	22.655217356201195
260-264	21.25903968812355	28.586060137714675	28.05394401448657	22.100956159675206
265-269	20.686554941696176	28.101348312014856	27.77811097911212	23.43398576717685
270-274	19.483915987128174	30.528540616601884	26.108407054128556	23.879136342141383
275-279	20.918905202938838	28.734847348934846	24.625436846172477	25.720810601953843
280-284	17.788779829529073	29.94434896944672	28.592230770117993	23.67464043090622
285-289	21.05160263434101	26.726104658539118	29.288993206537413	22.93329950058246
290-294	19.96066942711157	30.009515461182684	27.525461067346395	22.50435404435935
295-299	19.453005155649876	31.09295163838941	24.319096665551722	25.134946540408993
300-301	15.707287113182087	33.977981799517885	28.56440525484135	21.75032583245868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.5
30	0.25
31	2.5
32	4.75
33	7.25
34	22.0
35	64.25
36	186.75
37	708.5
38	2339.5
39	4317.5
40	7269.5
41	9515.25
42	13884.0
43	16160.25
44	17082.5
45	12088.25
46	8038.0
47	6558.5
48	5868.75
49	4895.25
50	3862.5
51	1751.75
52	430.0
53	92.0
54	40.5
55	21.5
56	19.75
57	17.75
58	13.0
59	21.25
60	31.0
61	20.25
62	7.5
63	3.0
64	2.25
65	1.25
66	0.5
67	0.25
68	0.75
69	0.5
70	0.25
71	0.0
72	0.25
73	0.25
74	0.25
75	0.25
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	346804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.737983589080613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.51910549730466	3.458844664315758
2	5.074635200540696	0.9883342860687221
3	4.263456404705423	1.2455240550534612
4	4.634712958108523	1.8053103490464022
5	4.597514889339984	2.2385262271473265
6	4.406130724466868	2.5744097211721337
7	3.900085375262348	2.658527716622263
8	4.347826642698455	3.387125159581201
9	3.490648817269384	3.0592692808031616
>10	28.998969010600323	48.68853669977209
>50	0.6368325434447455	3.945990543062325
>100	0.06224093298478663	0.946593202271719
>500	0.011798435352473921	0.7801232651983752
>1k	0.038344914893454395	9.856093417121311
>5k	0.014748044189790154	10.474792059671852
>10k+	0.0029496088379580305	3.89199935309185
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	13550	3.9071060310723063	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9679	2.790913599612461	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7718	2.2254645275141	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6982	2.0132409084093608	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	6462	1.8633003079549255	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5627	1.6225303053021303	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4608	1.3287043978731503	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4048	1.167229905076066	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4036	1.1637697373732714	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3670	1.0582346224380341	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3287	0.9477976032571712	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2545	0.7338439003010346	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2517	0.7257701756611804	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	2210	0.6372475519313503	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1775	0.5118164727050438	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1771	0.5106630834707789	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1421	0.40974152547260123	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1414	0.40772309431263765	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1012	0.2918074762690165	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	800	0.23067784685297746	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	706	0.20357319984775263	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	690	0.19895964291069307	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	520	0.14994060045443536	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	410	0.11822239651215097	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	8.650419256986655E-4	0.0	0.0
2	0.0011533892342648873	0.0	0.0
3	0.0014417365428311092	0.0	0.0
4	0.0014417365428311092	0.0	0.0
5	0.0014417365428311092	0.0	0.0
6	0.0014417365428311092	0.0	0.0
7	0.0014417365428311092	0.0	0.0
8	0.0023067784685297747	0.0	0.0
9	0.0025951257770959967	0.0	0.0
10-14	0.004325209628493328	0.0	0.0
15-19	0.004959573707339016	0.0	0.0
20-24	0.006228301865030391	0.0	0.0
25-29	0.006401310250170125	0.0	0.0
30-34	0.014878721122017047	0.0	0.0
35-39	0.015628424124289223	0.0	0.0
40-44	0.017358507975686555	0.0	0.0
45-49	0.021222361910473924	0.0	0.0
50-54	0.03062248416973276	0.0	0.0
55-59	0.039676589658712125	0.0	0.0
60-64	0.05051844846080207	0.0	0.0
65-69	0.06672356720222375	0.0	0.0
70-74	0.08731156503385197	0.0	0.0
75-79	0.11603095696704768	0.0	0.0
80-84	0.1535161070806565	0.0	0.0
85-89	0.19578782251646462	0.0	0.0
90-94	0.24619093205384024	0.0	0.0
95-99	0.297343744593488	0.0	0.0
100-104	0.3605494746312038	0.0	0.0
105-109	0.4307332095362222	0.0	0.0
110-114	0.4987255048961373	0.0	0.0
115-119	0.5770983033644365	0.0	0.0
120-124	0.6731179571169883	0.0	0.0
125-129	0.7682725689438415	0.0	0.0
130-134	0.8775561988904397	0.0	0.0
135-139	1.0013148637270621	0.0	0.0
140-144	1.1402405969942677	0.0	0.0
145-149	1.294448737615483	0.0	0.0
150-154	1.4637662772055686	0.0	0.0
155-159	1.6703382890624099	0.0	0.0
160-164	1.8863104231785102	0.0	0.0
165-169	2.1326743636174896	0.0	0.0
170-174	2.436477087922861	0.0	0.0
175-179	2.7523903991880143	0.0	0.0
180-184	3.088199674744236	0.0	0.0
185-189	3.5600512104820012	0.0	0.0
190-194	4.007220216606498	0.0	0.0
195-199	4.453178164035018	0.0	0.0
200-204	4.924395335693936	0.0	0.0
205-209	5.446880658815931	0.0	0.0
210-214	5.971557421483028	0.0	0.0
215-219	6.522762136538217	0.0	0.0
220-224	7.11525818618009	0.0	0.0
225-229	7.756542600431368	0.0	0.0
230-234	8.42193284967878	0.0	0.0
235-239	9.116676854938236	0.0	0.0
240-244	9.832066527491033	0.0	0.0
245-249	10.490421102409432	0.0	0.0
250-254	11.243584272384402	0.0	0.0
255-259	11.989538759645217	0.0	0.0
260-264	12.744547352395013	0.0	0.0
265-269	13.45745723809414	0.0	0.0
270-274	14.168637039941867	0.0	0.0
275-279	14.842216352752564	0.0	0.0
280-284	15.468795054266963	0.0	0.0
285-289	16.087242361679795	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGCGG	10	8.462483E-4	295.0	295
CGGGTTA	5475	0.0	293.65295	1
GGGGGGA	3780	0.0	288.7566	1
GGGTTAT	5585	0.0	288.13342	2
GTTATTA	5610	0.0	286.58646	4
GGTTATT	5625	0.0	285.82224	3
TTATTAG	5830	0.0	275.77188	5
TTAGTAG	5865	0.0	273.6232	8
GGGGAGC	4015	0.0	271.48816	3
GGGAGCG	3140	0.0	268.22452	4
GGAGCGA	3160	0.0	266.99368	5
ATTAGTA	6040	0.0	265.69534	7
TATTAGT	6105	0.0	262.86652	6
TAGTAGA	6205	0.0	258.63013	9
AAACGTT	280	0.0	258.12503	295
CGAAAGC	3320	0.0	253.68224	9
GAGCGAA	3425	0.0	245.9051	6
AGCGAAA	3515	0.0	239.18918	7
GGGGGAG	4590	0.0	237.47823	2
GCGAAAG	3600	0.0	234.36111	8
>>END_MODULE