FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_8561_flu.3510000003bac9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_8561_flu.3510000003bac9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346804
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC135503.9071060310723063No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT96792.790913599612461No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA77182.2254645275141No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG69822.0132409084093608No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG64621.8633003079549255No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA56271.6225303053021303No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT46081.3287043978731503No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT40481.167229905076066No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT40361.1637697373732714No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36701.0582346224380341No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32870.9477976032571712No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT25450.7338439003010346No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA25170.7257701756611804No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT22100.6372475519313503TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG17750.5118164727050438No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG17710.5106630834707789No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT14210.40974152547260123No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14140.40772309431263765No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10120.2918074762690165No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8000.23067784685297746No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7060.20357319984775263No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6900.19895964291069307No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT5200.14994060045443536No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT4100.11822239651215097No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGG108.462483E-4295.0295
CGGGTTA54750.0293.652951
GGGGGGA37800.0288.75661
GGGTTAT55850.0288.133422
GTTATTA56100.0286.586464
GGTTATT56250.0285.822243
TTATTAG58300.0275.771885
TTAGTAG58650.0273.62328
GGGGAGC40150.0271.488163
GGGAGCG31400.0268.224524
GGAGCGA31600.0266.993685
ATTAGTA60400.0265.695347
TATTAGT61050.0262.866526
TAGTAGA62050.0258.630139
AAACGTT2800.0258.12503295
CGAAAGC33200.0253.682249
GAGCGAA34250.0245.90516
AGCGAAA35150.0239.189187
GGGGGAG45900.0237.478232
GCGAAAG36000.0234.361118
Produced by FastQC (version 0.11.2)