##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_8033_flu.3510000003ba79.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440515
Sequences flagged as poor quality	0
Sequence length	301
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.76818269525442	34.0	34.0	34.0	34.0	34.0
2	33.85276551309263	34.0	34.0	34.0	34.0	34.0
3	33.87031315619218	34.0	34.0	34.0	34.0	34.0
4	33.88139109905452	34.0	34.0	34.0	34.0	34.0
5	33.86799087431756	34.0	34.0	34.0	34.0	34.0
6	37.5423878869051	38.0	38.0	38.0	37.0	38.0
7	37.57773969104344	38.0	38.0	38.0	37.0	38.0
8	37.5947266267891	38.0	38.0	38.0	37.0	38.0
9	37.56660045628412	38.0	38.0	38.0	37.0	38.0
10-14	37.655379271988465	38.0	38.0	38.0	37.6	38.0
15-19	37.60869754718909	38.0	38.0	38.0	37.0	38.0
20-24	37.60928231728772	38.0	38.0	38.0	37.4	38.0
25-29	37.507801096443934	38.0	38.0	38.0	37.0	38.0
30-34	37.43739123525874	38.0	38.0	38.0	37.0	38.0
35-39	37.35837099758238	38.0	38.0	38.0	37.0	38.0
40-44	37.34677774877133	38.0	38.0	38.0	37.0	38.0
45-49	37.331932397307696	38.0	38.0	38.0	37.0	38.0
50-54	37.282198336038505	38.0	38.0	38.0	37.0	38.0
55-59	37.2421315959729	38.0	38.0	38.0	37.0	38.0
60-64	37.0681579514886	38.0	38.0	38.0	36.6	38.0
65-69	37.012502638956676	38.0	38.0	38.0	36.4	38.0
70-74	37.07108180198177	38.0	38.0	38.0	37.0	38.0
75-79	36.97462333859234	38.0	38.0	38.0	36.2	38.0
80-84	36.8654883488644	38.0	38.0	38.0	35.8	38.0
85-89	36.89723800551627	38.0	38.0	38.0	36.0	38.0
90-94	36.78412040452652	38.0	38.0	38.0	35.8	38.0
95-99	36.78003609411711	38.0	38.0	38.0	35.8	38.0
100-104	36.75852263827566	38.0	38.0	38.0	35.8	38.0
105-109	36.62348160675573	38.0	38.0	38.0	35.0	38.0
110-114	36.52585178711281	38.0	37.8	38.0	34.4	38.0
115-119	36.58333314416081	38.0	37.8	38.0	34.8	38.0
120-124	36.428169301839894	38.0	37.2	38.0	34.0	38.0
125-129	36.35637515181095	38.0	37.0	38.0	34.0	38.0
130-134	36.252132617504515	38.0	37.0	38.0	34.0	38.0
135-139	36.07764729918391	38.0	37.0	38.0	33.2	38.0
140-144	35.95956959467895	38.0	37.0	38.0	32.0	38.0
145-149	35.5225116057342	38.0	36.4	38.0	29.4	38.0
150-154	35.26886394333904	38.0	36.4	38.0	28.4	38.0
155-159	35.2592225009364	38.0	36.0	38.0	28.2	38.0
160-164	35.2438736478894	38.0	36.0	38.0	28.0	38.0
165-169	34.96117362632374	38.0	35.4	38.0	27.2	38.0
170-174	34.81525214805398	38.0	35.0	38.0	26.4	38.0
175-179	34.488631715151584	38.0	35.0	38.0	24.0	38.0
180-184	34.1470987367059	37.6	34.6	38.0	23.0	38.0
185-189	33.404625041145025	37.0	33.6	37.8	21.8	38.0
190-194	32.45138894248777	36.8	30.4	37.0	20.2	38.0
195-199	32.19607504852276	36.8	29.8	37.2	20.0	37.8
200-204	32.04554214952953	36.8	29.8	37.0	19.4	37.4
205-209	31.098427976345867	35.4	27.6	37.0	16.8	37.2
210-214	30.365108112096074	35.0	26.2	37.0	15.4	37.0
215-219	29.6861632407523	34.2	24.8	37.0	13.4	37.0
220-224	28.29049362677775	32.2	22.2	36.2	9.4	37.0
225-229	27.432038863602827	31.0	20.8	36.0	9.0	37.0
230-234	27.060847417227563	30.8	20.2	36.0	9.0	37.0
235-239	26.18766489222842	28.8	19.4	35.6	9.0	37.0
240-244	25.217715174284642	27.6	17.6	34.2	8.8	37.0
245-249	24.402287322792642	26.0	16.2	34.0	8.2	36.4
250-254	23.470428475761327	25.0	14.8	33.0	8.0	36.0
255-259	22.991144001906857	24.0	14.0	32.0	8.0	36.0
260-264	22.034634461936598	22.4	12.8	31.2	8.0	36.0
265-269	21.196952657684754	21.0	11.6	29.8	8.0	35.8
270-274	20.19701440359579	19.8	10.2	28.8	7.4	34.2
275-279	19.001197689068476	18.4	9.0	26.8	7.0	33.6
280-284	18.632948707762505	18.2	9.0	26.0	7.0	32.8
285-289	17.46033892148962	16.4	8.2	24.2	7.0	30.8
290-294	17.065116511356027	15.4	8.0	23.4	7.0	30.6
295-299	16.12975358387342	14.0	8.0	22.0	7.0	29.2
300-301	14.366299671974847	12.0	8.0	18.5	7.0	25.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.046967262831088874
1101	2	0.03264021584758581
1101	3	0.039438531730731086
1101	4	0.043839734108729544
1101	5	-8.313275544296062E-4
1101	6	0.17159072889841553
1101	7	0.176392102963689
1101	8	0.18715617816555152
1101	9	0.11633840104090609
1101	10-14	0.023320764466213006
1101	15-19	0.07184065855947352
1101	20-24	0.07174832140921694
1101	25-29	0.15320399042545318
1101	30-34	0.14793760121005306
1101	35-39	0.1882141687026575
1101	40-44	0.1435189804705601
1101	45-49	0.17574886238865872
1101	50-54	0.18313492656444907
1101	55-59	0.1775792875302713
1101	60-64	0.3635734255784584
1101	65-69	0.33865264734816947
1101	70-74	0.292054575083057
1101	75-79	0.19939510714868192
1101	80-84	0.284562073499373
1101	85-89	0.2469641925791919
1101	90-94	0.3291349424134751
1101	95-99	0.26535008162341
1101	100-104	0.2883175059770835
1101	105-109	0.21656175029004743
1101	110-114	0.2564022447806664
1101	115-119	0.2432784696203001
1101	120-124	0.28823690966601845
1101	125-129	0.30028223569145496
1101	130-134	0.349938477021972
1101	135-139	0.31266787892463554
1101	140-144	0.3027930078980887
1101	145-149	0.5204102966609909
1101	150-154	0.8108509681144085
1101	155-159	0.5764590708796717
1101	160-164	0.42214316986925127
1101	165-169	0.2720052127435366
1101	170-174	0.507485722336213
1101	175-179	0.41871214426059566
1101	180-184	0.22059843186322325
1101	185-189	0.3237365415996081
1101	190-194	0.5588175268731987
1101	195-199	0.28809504135134745
1101	200-204	0.10605916052044506
1101	205-209	0.0723305082664254
1101	210-214	0.029660077057169332
1101	215-219	-0.06805704267269164
1101	220-224	0.418619525970211
1101	225-229	0.9055316781660601
1101	230-234	0.6387199083735133
1101	235-239	0.6262584189316733
1101	240-244	0.6597064988388155
1101	245-249	0.7183507012876476
1101	250-254	0.589112487423364
1101	255-259	0.15668722781122213
1101	260-264	0.8787391544569907
1101	265-269	0.4719586298041598
1101	270-274	0.43455750598291587
1101	275-279	0.8861666118846152
1101	280-284	0.13623692137073817
1101	285-289	0.5478194476378349
1101	290-294	0.4461326514647048
1101	295-299	0.22247892760710286
1101	300-301	0.33275798922664634
1102	1	-0.14411182324776206
1102	2	-0.0693482397422116
1102	3	-0.010572149150938515
1102	4	0.021549526627879345
1102	5	0.01258884172433028
1102	6	0.03474032842196095
1102	7	0.11216565921419885
1102	8	0.02827296813413227
1102	9	0.02459981769568742
1102	10-14	0.10437423907402632
1102	15-19	0.07395328411644897
1102	20-24	0.054417062355945234
1102	25-29	0.034658734057885
1102	30-34	0.07409111756010134
1102	35-39	0.008308870004064772
1102	40-44	0.05281331009238954
1102	45-49	0.17256829963074694
1102	50-54	0.11348078332590461
1102	55-59	-0.002577823332750029
1102	60-64	0.20177535387387735
1102	65-69	0.2839629099770917
1102	70-74	0.16482934736892219
1102	75-79	0.07835407218257728
1102	80-84	0.12182816030362176
1102	85-89	0.22127738924251616
1102	90-94	0.27803700061559766
1102	95-99	0.13138495338112222
1102	100-104	0.1413941660238649
1102	105-109	0.17288607771531161
1102	110-114	0.24031993179233524
1102	115-119	0.150426961049547
1102	120-124	0.25116572064612086
1102	125-129	0.11705673019891805
1102	130-134	0.19915537591289478
1102	135-139	0.18156069797539942
1102	140-144	0.36849484750069195
1102	145-149	0.4382438968276503
1102	150-154	0.8121596969234304
1102	155-159	0.46880484159020597
1102	160-164	0.5479917338379181
1102	165-169	0.6857359201651292
1102	170-174	0.545823218571492
1102	175-179	0.7364100255208967
1102	180-184	0.8032487548938434
1102	185-189	0.8186501019413086
1102	190-194	0.7956106355532455
1102	195-199	0.8812428217282644
1102	200-204	0.6425078390727066
1102	205-209	0.3379970217020656
1102	210-214	0.0582575150042679
1102	215-219	0.2306681205032497
1102	220-224	1.077833277209077
1102	225-229	1.1277978819693644
1102	230-234	0.9884460928380001
1102	235-239	1.1411792709565525
1102	240-244	1.2647659909723288
1102	245-249	1.1725765185260641
1102	250-254	0.8295283398143347
1102	255-259	0.7760674045477316
1102	260-264	1.3137325911873
1102	265-269	1.3650636111711165
1102	270-274	1.5158934389598144
1102	275-279	1.5056142706579934
1102	280-284	0.94156151072292
1102	285-289	1.5957211261591482
1102	290-294	1.2753049699406667
1102	295-299	1.3877919529234362
1102	300-301	1.1235466911938161
1103	1	-0.013084956490061472
1103	2	-0.03176361356929647
1103	3	-0.017132399513826613
1103	4	0.027303615744934007
1103	5	0.044425417441217974
1103	6	0.0923914251560305
1103	7	0.09371151114471843
1103	8	0.04181240096799144
1103	9	0.13026355334726247
1103	10-14	0.03985688283001565
1103	15-19	0.019795401814484137
1103	20-24	0.12118261209676717
1103	25-29	0.05311695822354068
1103	30-34	0.15542237057471908
1103	35-39	-0.3475560662842838
1103	40-44	-0.13428780804117935
1103	45-49	-0.014329466593061113
1103	50-54	0.14536295093346752
1103	55-59	-0.16393507278304753
1103	60-64	0.2793262541250172
1103	65-69	0.3612810198460039
1103	70-74	0.197885732611347
1103	75-79	0.36736725684407645
1103	80-84	0.34008861832096215
1103	85-89	0.299531642535662
1103	90-94	0.42574068653880204
1103	95-99	0.4221821094737166
1103	100-104	0.40789975140788926
1103	105-109	0.2061178860602837
1103	110-114	0.3832951951723089
1103	115-119	0.2975865636150772
1103	120-124	0.5173056651055319
1103	125-129	0.4795685716357525
1103	130-134	0.6129497912082229
1103	135-139	0.5615799764007008
1103	140-144	0.6570140696909519
1103	145-149	0.9352057709864923
1103	150-154	1.0938796887410405
1103	155-159	0.23860006304677484
1103	160-164	-0.27098128617948447
1103	165-169	0.7397162658331808
1103	170-174	0.9682341992726862
1103	175-179	0.7685815959577269
1103	180-184	0.8616776311669625
1103	185-189	0.9300028601374635
1103	190-194	0.6103405904067074
1103	195-199	0.823343083126808
1103	200-204	0.7532306139582161
1103	205-209	0.9868648859861757
1103	210-214	0.7948471962912826
1103	215-219	0.7993058816092962
1103	220-224	1.267705687850107
1103	225-229	1.849657004536816
1103	230-234	1.132540622037574
1103	235-239	1.6128554598368119
1103	240-244	1.2275045841303758
1103	245-249	1.2981592304434386
1103	250-254	1.3596956031761955
1103	255-259	0.6901946255483864
1103	260-264	1.5696007732559494
1103	265-269	0.5701309535639467
1103	270-274	1.0879952779585444
1103	275-279	1.7183685265930535
1103	280-284	0.6234431876892792
1103	285-289	0.8872450351051988
1103	290-294	1.1458715548589602
1103	295-299	1.10133010254183
1103	300-301	-1.0871723850118222
2116	1	-0.02089553871317662
2116	2	-0.07835262073271565
2116	3	-0.05733681360857901
2116	4	-0.036013426945480376
2116	5	-0.09357864406153737
2116	6	-0.4964773793785682
2116	7	-0.22534184628984377
2116	8	-0.04340774587095808
2116	9	-0.013205684116755378
2116	10-14	0.07066003384204578
2116	15-19	0.0857622403139473
2116	20-24	-0.1862093229191828
2116	25-29	-0.29558581564894126
2116	30-34	-0.00275575533186867
2116	35-39	-0.19290698458559774
2116	40-44	-0.22726230091123512
2116	45-49	-0.08908254061505971
2116	50-54	0.08823412095868122
2116	55-59	0.0426766966485701
2116	60-64	0.24442455588128098
2116	65-69	0.1112589270562836
2116	70-74	-0.9681386810370896
2116	75-79	0.11000053885072703
2116	80-84	-0.29075336244269323
2116	85-89	0.018555096598291243
2116	90-94	0.23211903114168564
2116	95-99	0.1523804982621897
2116	100-104	0.08809211489006685
2116	105-109	0.13414314771478075
2116	110-114	0.3364874917066487
2116	115-119	0.20472855999989292
2116	120-124	-0.37864443643204027
2116	125-129	-0.12805946612282781
2116	130-134	0.053803599424959714
2116	135-139	0.341022970308444
2116	140-144	0.36322861522665306
2116	145-149	0.48987716863438635
2116	150-154	0.8220236712453399
2116	155-159	-0.3917955987326991
2116	160-164	-0.5056834798372947
2116	165-169	0.6102235073587039
2116	170-174	0.7171579010423415
2116	175-179	0.5257247081400038
2116	180-184	0.6438275497137482
2116	185-189	1.1465019340255722
2116	190-194	0.9257131551787552
2116	195-199	0.8490965811518478
2116	200-204	0.8085018643208528
2116	205-209	0.6224124078000237
2116	210-214	0.7098801123163696
2116	215-219	0.9327191060407962
2116	220-224	1.5197183633092592
2116	225-229	1.300602531304797
2116	230-234	1.4372925802621133
2116	235-239	1.0778350407432882
2116	240-244	1.5124640806825553
2116	245-249	1.310053631371776
2116	250-254	1.1577853579239097
2116	255-259	1.261826590914037
2116	260-264	0.4958537022242844
2116	265-269	0.8699823293422178
2116	270-274	1.1854521523866985
2116	275-279	1.7455439969016844
2116	280-284	-0.007753616666796859
2116	285-289	-1.0776841117560636
2116	290-294	0.433600460707968
2116	295-299	0.8466935177746961
2116	300-301	1.2517466304084977
1117	1	-0.03753168034931775
1117	2	0.04688285807419135
1117	3	0.03882575398255739
1117	4	0.051510557007077296
1117	5	0.055457637117598324
1117	6	-0.38438074585336324
1117	7	-0.3292272466038142
1117	8	-0.0429019025079711
1117	9	0.027283588515373935
1117	10-14	0.08783516030288041
1117	15-19	0.02842402297573443
1117	20-24	-0.011912270764987909
1117	25-29	0.09137737988567807
1117	30-34	0.03175671632347132
1117	35-39	-0.09057661654094318
1117	40-44	-0.07551975774012476
1117	45-49	0.10776117122864548
1117	50-54	0.09325330812072963
1117	55-59	0.18555738738639604
1117	60-64	0.18752415469516848
1117	65-69	0.20613857122165058
1117	70-74	-0.1872499665769496
1117	75-79	0.18261609789485078
1117	80-84	0.02447077849573276
1117	85-89	0.1434234028951593
1117	90-94	0.19845253890376569
1117	95-99	0.2703100290488578
1117	100-104	0.2791613631021761
1117	105-109	0.28657294014457335
1117	110-114	0.32951906330964675
1117	115-119	0.3177767023337452
1117	120-124	0.4633510284205684
1117	125-129	0.4266867400519416
1117	130-134	0.1923218272288878
1117	135-139	0.32901616758736196
1117	140-144	0.3092054162034472
1117	145-149	0.8174081297367763
1117	150-154	0.8739764010552094
1117	155-159	-0.12085891636744606
1117	160-164	-0.6305796947334983
1117	165-169	0.27886121885354953
1117	170-174	0.5798676516092414
1117	175-179	0.8231876420315061
1117	180-184	0.640472412787183
1117	185-189	0.9191349359442924
1117	190-194	1.0640909678422759
1117	195-199	0.7867522588075175
1117	200-204	1.0253159232777662
1117	205-209	1.5044445026892639
1117	210-214	0.937497415647961
1117	215-219	0.7127705625208236
1117	220-224	1.4070513477850994
1117	225-229	0.7181526795692363
1117	230-234	1.2763742129151758
1117	235-239	1.0074661239458003
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2108	160-164	-0.2528367665763298
2108	165-169	-0.08677123130961206
2108	170-174	-0.6372985106303091
2108	175-179	-0.7724792162611038
2108	180-184	-0.4536853911794836
2108	185-189	-0.7103874363849272
2108	190-194	-0.6003990535183839
2108	195-199	-1.3195759062613206
2108	200-204	-0.8152190230951426
2108	205-209	-0.43898216375196597
2108	210-214	-0.4742286832423055
2108	215-219	-0.6202222120537932
2108	220-224	-0.43617326600407935
2108	225-229	-0.18563855523454365
2108	230-234	-0.2683246525465002
2108	235-239	-0.5314016766955838
2108	240-244	-0.33417326981560436
2108	245-249	-0.20503428172773752
2108	250-254	-0.42042154058124837
2108	255-259	-0.626073762437855
2108	260-264	0.02309522071683645
2108	265-269	-0.8346067132087143
2108	270-274	-0.8671700868304058
2108	275-279	-0.1971509662406632
2108	280-284	-0.5388093527929563
2108	285-289	-0.08975784121173191
2108	290-294	-0.4602323804819548
2108	295-299	-0.1038791388791509
2108	300-301	-0.3026596463672888
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	50.0
13	199.0
14	532.0
15	693.0
16	702.0
17	758.0
18	1320.0
19	1214.0
20	1111.0
21	1789.0
22	2310.0
23	3397.0
24	4692.0
25	6855.0
26	9543.0
27	12893.0
28	17588.0
29	24694.0
30	33441.0
31	44578.0
32	60901.0
33	78809.0
34	80937.0
35	45447.0
36	6047.0
37	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.369976050758773	21.822866417715627	16.954246733936415	32.85291079758919
2	26.52145783912012	24.8554532762789	17.58419123071859	31.038897653882387
3	42.988774502570855	24.141516179925766	20.05175760189778	12.817951715605597
4	44.01461925246586	23.658218221853964	19.502854613350284	12.824307912329886
5	42.61716400122584	23.77558085422744	20.386819972078136	13.220435172468589
6	24.25184159449735	24.746943917914262	37.1524238675187	13.848790620069693
7	16.775630286438115	31.87866900938449	37.794485532809404	13.551215171367993
8	23.73789768793344	38.41503694539346	22.602408544544453	15.244656822128643
9	14.403595791289742	21.037422108214248	40.85036831889947	23.70861378159654
10-14	22.704198494943416	34.22603089565622	28.099020464683385	14.970750144716979
15-19	22.576302736569698	33.971238209822594	22.789371531048886	20.663087522558822
20-24	21.935643508166578	30.424208029238503	27.402472106511695	20.23767635608322
25-29	17.515544306096274	28.184511310625066	33.41044005311964	20.889504330159017
30-34	17.56376059839052	30.30030759452005	31.631068181560217	20.50486362552921
35-39	20.386774570672962	30.17963065956891	30.942692076319762	18.490902693438365
40-44	18.839812492196632	29.755218324007128	31.451868835340452	19.953100348455784
45-49	20.43031451823434	28.248708897540382	31.55679148269639	19.76418510152889
50-54	18.86905099712836	31.17591909469598	31.078805489029886	18.876224419145775
55-59	20.786806351656583	31.028455330692484	28.308366343938346	19.876371973712587
60-64	19.159211377592136	30.020271727409963	29.191741484398943	21.62877541059896
65-69	18.649943815761098	31.17119734855794	29.94068306414083	20.238175771540128
70-74	19.470755819892627	32.18482912046128	27.06632010260718	21.27809495703892
75-79	19.355890264803698	32.64987571365334	28.387092380509177	19.60714164103379
80-84	19.745888335243976	31.633610660249932	26.175635335913643	22.444865668592442
85-89	20.599026139859028	31.834012462685717	26.229072789802842	21.337888607652406
90-94	19.474160925280636	29.833217938095185	29.52739407284655	21.165227063777625
95-99	20.004222330681134	29.378840675118894	29.246223170607134	21.37071382359284
100-104	18.998399600467632	28.663042121153648	30.114252636119087	22.22430564225963
105-109	20.09938367592477	27.78003019193444	31.873103072540093	20.247483059600693
110-114	18.686037932874022	28.857314733891016	29.381337752403436	23.075309580831526
115-119	18.14930252091302	29.729657332894455	30.207870333586822	21.9131698126057
120-124	21.319001623100235	28.602522048057388	29.099304223465715	20.979172105376662
125-129	21.348103923816442	28.525521264883146	29.930921762028532	20.195453049271876
130-134	20.769009000828575	29.010998490403278	29.099849040327797	21.120143468440347
135-139	19.70416444388954	27.474569537927202	31.38994131868381	21.43132469949945
140-144	19.98687899390486	29.0231206655846	29.54827871922636	21.44172162128418
145-149	19.116670260944577	30.335720690555373	28.72655868699136	21.82105036150869
150-154	18.96348591988922	28.56965143071178	28.89177440041769	23.575088248981306
155-159	20.059385037966926	29.80012031372371	30.549833717353554	19.590660930955814
160-164	20.16503410780564	28.383369465284954	28.618230934247414	22.833365492661997
165-169	20.66408635347264	28.428680067648095	29.139166657208044	21.768066921671224
170-174	18.770121335255325	30.198381439905564	31.226723267085116	19.804773957753994
175-179	19.62430337218937	31.477021213806566	28.337695651680416	20.560979762323644
180-184	21.049998297447306	27.800279218641816	29.95348626039976	21.196236223511118
185-189	19.768543636425548	29.676583090246645	28.612510357195553	21.942362916132254
190-194	20.318808667128245	29.240230185124226	28.893590456624636	21.547370691122893
195-199	19.549164046627244	30.876088214930252	30.780654461255573	18.79409327718693
200-204	20.97826407727319	27.83206020226326	29.777056399895578	21.41261932056797
205-209	20.13643122254634	28.550174227892356	30.185215032405253	21.128179517156056
210-214	20.27681236734277	27.141958843626213	29.84829120461278	22.732937584418238
215-219	19.32692416830301	29.36789893647208	29.237960114865558	22.067216780359352
220-224	20.30945597766251	28.59330556280717	30.340896450745152	20.75634200878517
225-229	18.91200072642248	29.731382586291044	29.909855510028034	21.446761177258438
230-234	17.761529119326244	30.281466011373055	27.780802015822392	24.176202853478316
235-239	19.465807066728715	29.593316913158464	27.982202649171995	22.95867337094083
240-244	18.174818110620524	29.564214612442257	29.635994234021545	22.624973042915677
245-249	20.132299694675552	29.271920365935326	28.377966698069308	22.21781324131982
250-254	21.035787657628003	29.94840130302033	27.101733198642496	21.91407784070917
255-259	19.099599332599343	29.514908686423848	27.339273350510197	24.046218630466612
260-264	17.508552489699554	31.236938583249152	27.716286619070864	23.538222307980433
265-269	19.518064084083402	30.641226745967785	26.914270796681155	22.92643837326765
270-274	18.4137657060486	29.835079395707297	28.512082448951798	23.239072449292305
275-279	18.85874487815398	30.577573975914557	26.12560298741246	24.438078158519005
280-284	18.287867609502513	29.831174874862377	27.934576575144998	23.94638094049011
285-289	18.786011827066048	30.586336447113037	27.1106318740565	23.517019851764413
290-294	17.068295063732222	32.075547938208686	26.711780529607392	24.144376468451696
295-299	18.74910048466	30.14040384549902	25.31373506010011	25.796760609740872
300-301	14.32834296221468	32.14362734526633	33.439156441891875	20.088873250627106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.25
15	0.0
16	0.25
17	1.75
18	1.25
19	1.25
20	0.5
21	1.25
22	1.75
23	0.75
24	1.75
25	1.75
26	0.75
27	4.25
28	4.5
29	8.5
30	20.0
31	61.75
32	358.25
33	1261.75
34	3450.25
35	5386.0
36	7926.25
37	8991.0
38	9934.5
39	10608.75
40	13560.75
41	16704.0
42	13675.25
43	11493.5
44	11134.0
45	9278.25
46	6167.75
47	3717.25
48	2394.25
49	2197.5
50	2986.5
51	2627.5
52	1008.75
53	428.0
54	259.75
55	184.5
56	159.5
57	162.0
58	154.75
59	155.75
60	161.0
61	102.0
62	39.5
63	18.0
64	11.0
65	6.5
66	5.25
67	3.0
68	1.75
69	1.25
70	1.0
71	0.75
72	0.75
73	0.5
74	0.25
75	0.25
76	0.25
77	0.25
78	0.25
79	0.0
80	0.0
81	0.25
82	0.25
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.25
89	0.25
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.270070258674506E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	440515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.296782502827206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.768355790724144	5.751002991733741
2	7.139968669804313	1.7559728361916822
3	4.148417662154142	1.5303656916718924
4	3.5234824113812153	1.733099874611678
5	3.455389125832615	2.1245084271498946
6	3.4015633372677123	2.5096970716783273
7	3.0565374703896167	2.630990353958594
8	2.5355185346774785	2.494297596225288
9	2.6676702961424454	2.9523385278824668
>10	22.690887579240076	50.20861062917298
>50	0.3715963954012374	2.9016638790283116
>100	0.163589183014211	4.155893735916504
>500	0.01833893939880614	1.6644889236016591
>1k	0.05501681678630782	14.554411743144666
>5k	0.003667787785753851	3.0326577180323726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	8216	1.8650897245269742	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	5232	1.1877007593385014	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT	4911	1.11483150403505	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA	3886	0.882149302520913	No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC	3772	0.8562705015720237	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	3465	0.7865793446307162	No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA	3165	0.7184772368704812	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	2869	0.6512831572137158	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2743	0.622680271954417	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	2738	0.6215452368250798	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	2551	0.5790949229878665	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	2404	0.5457248901853512	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2370	0.5380066513058579	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	2236	0.5075877098396195	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2134	0.4844329932011396	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT	2068	0.4694505294938879	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	2053	0.4660454241058761	No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA	2048	0.4649103889765388	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1675	0.38023676832797976	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1620	0.36775138190526996	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	1615	0.3666163467759327	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1594	0.36184919923271625	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	1593	0.3616221922068488	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	1440	0.32689011724912886	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT	1415	0.32121494160244257	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1288	0.2923850493172764	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1244	0.28239674017910854	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1171	0.2658252272907846	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1147	0.26037705866996586	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1136	0.2578799813854239	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA	1105	0.2508427635835329	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	1084	0.24607561604031644	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	984	0.22337491345357138	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	945	0.2145216394447408	No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA	867	0.19681509142707967	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	838	0.19023188767692362	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	694	0.15754287595201072	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	663	0.15050565815011976	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	653	0.14823558789144523	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG	614	0.13938231388261468	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	592	0.13438815931353074	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	531	0.12054073073561626	No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	467	0.10601228108009941	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	460	0.10442323189902727	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	455	0.10328819676969003	No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC	453	0.10283418271795514	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	442	0.10033710543341316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	2.270070258674506E-4	0.0	0.0
7	2.270070258674506E-4	0.0	0.0
8	4.540140517349012E-4	0.0	0.0
9	6.810210776023518E-4	0.0	0.0
10-14	0.002542478689715447	0.0	0.0
15-19	0.0032234997673177986	0.0	0.0
20-24	0.0036321124138792094	0.0	0.0
25-29	0.00399532365526713	0.0	0.0
30-34	0.010215316164035277	0.0	0.0
35-39	0.01480085808655778	0.0	0.0
40-44	0.07114400190685902	0.0	0.0
45-49	0.17225293122822152	0.0	0.0
50-54	0.18269525441812423	0.0	0.0
55-59	0.1975415139098555	0.0	0.0
60-64	0.2261443991691543	0.0	0.0
65-69	0.3025095626709646	0.0	0.0
70-74	0.3850039158711962	0.0	0.0
75-79	0.43944020067421086	0.0	0.0
80-84	0.5133536882966527	0.0	0.0
85-89	0.5914441051950559	0.0	0.0
90-94	0.6860152321714357	0.0	0.0
95-99	0.8039226814069895	0.0	0.0
100-104	0.9392642702291637	0.0	0.0
105-109	1.093538245008683	0.0	0.0
110-114	1.2609786272885146	0.0	0.0
115-119	1.434048783809859	0.0	0.0
120-124	1.6404889731337184	0.0	0.0
125-129	1.862411041621738	0.0	0.0
130-134	2.087692814092596	0.0	0.0
135-139	2.315244656822128	0.0	0.0
140-144	2.634416535191764	0.0	0.0
145-149	3.0850254815386533	0.0	0.0
150-154	3.4472832934179314	0.0	0.0
155-159	4.253294439462902	0.0	0.0
160-164	4.546178904237086	0.0	0.0
165-169	5.020351179869016	0.0	0.0
170-174	5.533387058329455	0.0	0.0
175-179	5.896325891286335	0.0	0.0
180-184	6.272703540174568	0.0	0.0
185-189	6.71318797316777	0.0	0.0
190-194	7.18045923521333	0.0	0.0
195-199	7.716967640148463	0.0	0.0
200-204	8.306232477895191	0.0	0.0
205-209	8.928640341418568	0.0	0.0
210-214	9.619014108486656	0.0	0.0
215-219	10.338626380486476	0.0	0.0
220-224	11.14481913215214	0.0	0.0
225-229	11.950240059929856	0.0	0.0
230-234	12.82707739804547	0.0	0.0
235-239	13.729430325868586	0.0	0.0
240-244	14.67300772958923	0.0	0.0
245-249	15.633474456034415	0.0	0.0
250-254	16.70753549822367	0.0	0.0
255-259	17.70995312304916	0.0	0.0
260-264	18.741609252806377	0.0	0.0
265-269	19.691906064492695	0.0	0.0
270-274	20.67330283872286	0.0	0.0
275-279	21.67749111835011	0.0	0.0
280-284	22.620886916450065	0.0	0.0
285-289	23.511344676117723	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGAGG	10	8.4632577E-4	295.0	295
CCGGGTT	7500	0.0	291.06668	1
CGGGTTA	7655	0.0	284.59506	2
GGGGGGA	4115	0.0	263.8153	1
TTATTAG	8395	0.0	258.45444	6
GTTATTA	8415	0.0	258.01544	5
GGGTTAT	8510	0.0	255.82845	3
TATTAGT	8805	0.0	246.25214	7
ATTAGTA	8895	0.0	243.59471	8
GGGGAGC	4595	0.0	236.25679	3
GGGAGCA	4665	0.0	232.3955	4
GGAGCAG	4710	0.0	229.862	5
TTAGTAG	9475	0.0	228.37204	9
GAGCAGA	4850	0.0	222.92268	6
GGTTATT	9805	0.0	221.88934	4
CAGAAGC	4995	0.0	216.45146	9
GGGGGAG	5095	0.0	213.36115	2
GCAGAAG	5725	0.0	189.10918	8
CGTCACG	25	5.5541866E-5	177.0	295
AGCAGAA	6680	0.0	162.29417	7
>>END_MODULE