FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_8033_flu.3510000003ba79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_8033_flu.3510000003ba79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440515
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT82161.8650897245269742No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG52321.1877007593385014No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT49111.11483150403505No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA38860.882149302520913No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC37720.8562705015720237No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT34650.7865793446307162No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA31650.7184772368704812No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT28690.6512831572137158No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT27430.622680271954417No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA27380.6215452368250798No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT25510.5790949229878665No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA24040.5457248901853512No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG23700.5380066513058579No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT22360.5075877098396195No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC21340.4844329932011396No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT20680.4694505294938879No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG20530.4660454241058761No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA20480.4649103889765388No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT16750.38023676832797976No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC16200.36775138190526996No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT16150.3666163467759327TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA15940.36184919923271625No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT15930.3616221922068488No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC14400.32689011724912886No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT14150.32121494160244257No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12880.2923850493172764No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC12440.28239674017910854No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG11710.2658252272907846No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11470.26037705866996586No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA11360.2578799813854239No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA11050.2508427635835329No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG10840.24607561604031644No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA9840.22337491345357138No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA9450.2145216394447408No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA8670.19681509142707967No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA8380.19023188767692362No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6940.15754287595201072No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6630.15050565815011976No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6530.14823558789144523No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG6140.13938231388261468No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT5920.13438815931353074No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5310.12054073073561626No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA4670.10601228108009941No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA4600.10442323189902727No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA4550.10328819676969003No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC4530.10283418271795514No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG4420.10033710543341316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGG108.4632577E-4295.0295
CCGGGTT75000.0291.066681
CGGGTTA76550.0284.595062
GGGGGGA41150.0263.81531
TTATTAG83950.0258.454446
GTTATTA84150.0258.015445
GGGTTAT85100.0255.828453
TATTAGT88050.0246.252147
ATTAGTA88950.0243.594718
GGGGAGC45950.0236.256793
GGGAGCA46650.0232.39554
GGAGCAG47100.0229.8625
TTAGTAG94750.0228.372049
GAGCAGA48500.0222.922686
GGTTATT98050.0221.889344
CAGAAGC49950.0216.451469
GGGGGAG50950.0213.361152
GCAGAAG57250.0189.109188
CGTCACG255.5541866E-5177.0295
AGCAGAA66800.0162.294177
Produced by FastQC (version 0.11.2)