##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7965_flu.3510000003b951.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225577
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.779064354965264	34.0	34.0	34.0	34.0	34.0
2	33.87483209724396	34.0	34.0	34.0	34.0	34.0
3	33.87893269260607	34.0	34.0	34.0	34.0	34.0
4	33.89239594462202	34.0	34.0	34.0	34.0	34.0
5	33.89045869038067	34.0	34.0	34.0	34.0	34.0
6	37.663853141056045	38.0	38.0	38.0	38.0	38.0
7	37.67441716132407	38.0	38.0	38.0	38.0	38.0
8	37.66086968086285	38.0	38.0	38.0	38.0	38.0
9	37.575657092700055	38.0	38.0	38.0	37.0	38.0
10-14	37.66296829907304	38.0	38.0	38.0	38.0	38.0
15-19	37.63511882860398	38.0	38.0	38.0	37.6	38.0
20-24	37.628838046432044	38.0	38.0	38.0	37.4	38.0
25-29	37.5318591877718	38.0	38.0	38.0	37.0	38.0
30-34	37.466846354016596	38.0	38.0	38.0	37.0	38.0
35-39	37.376729897108305	38.0	38.0	38.0	37.0	38.0
40-44	37.38229606741822	38.0	38.0	38.0	37.0	38.0
45-49	37.349387570541325	38.0	38.0	38.0	37.0	38.0
50-54	37.31445227128652	38.0	38.0	38.0	37.0	38.0
55-59	37.26503765898119	38.0	38.0	38.0	37.0	38.0
60-64	37.104318259397004	38.0	38.0	38.0	36.6	38.0
65-69	37.03404513758052	38.0	38.0	38.0	36.4	38.0
70-74	37.10985694463532	38.0	38.0	38.0	37.0	38.0
75-79	37.03752598890844	38.0	38.0	38.0	36.8	38.0
80-84	36.928520194878026	38.0	38.0	38.0	36.0	38.0
85-89	36.96858013006645	38.0	38.0	38.0	36.0	38.0
90-94	36.827261644582556	38.0	38.0	38.0	35.8	38.0
95-99	36.82994897529447	38.0	38.0	38.0	36.0	38.0
100-104	36.813268196668986	38.0	38.0	38.0	36.0	38.0
105-109	36.66423083913697	38.0	38.0	38.0	35.0	38.0
110-114	36.571228449708975	38.0	38.0	38.0	34.6	38.0
115-119	36.669409558598616	38.0	38.0	38.0	35.0	38.0
120-124	36.574755405028	38.0	37.8	38.0	34.8	38.0
125-129	36.49086653337885	38.0	37.0	38.0	34.0	38.0
130-134	36.35762688571973	38.0	37.0	38.0	34.0	38.0
135-139	36.12660865247786	38.0	37.0	38.0	33.4	38.0
140-144	36.09128856222044	38.0	37.0	38.0	33.2	38.0
145-149	35.67824822566131	38.0	37.0	38.0	30.2	38.0
150-154	35.30629895778382	38.0	36.2	38.0	28.0	38.0
155-159	35.40539682680416	38.0	36.0	38.0	29.0	38.0
160-164	35.35997286957447	38.0	36.0	38.0	28.6	38.0
165-169	35.17634155964482	38.0	35.6	38.0	28.2	38.0
170-174	35.03821754877492	38.0	35.4	38.0	28.0	38.0
175-179	34.6787562561786	38.0	35.0	38.0	25.2	38.0
180-184	34.41243211852272	37.6	35.0	38.0	23.8	38.0
185-189	33.66732335300141	37.0	33.8	37.8	22.8	38.0
190-194	32.68938411274199	36.8	31.2	37.0	21.2	38.0
195-199	32.46963741870847	36.8	30.4	37.2	20.6	37.8
200-204	32.27124219224478	37.0	30.0	37.0	20.0	37.4
205-209	31.23294750794629	35.4	28.2	37.0	18.2	37.2
210-214	30.554122982396258	34.6	26.6	37.0	16.6	37.0
215-219	29.700245149106514	33.4	24.8	37.0	14.6	37.0
220-224	28.27470176480758	31.8	22.4	36.2	9.8	37.0
225-229	27.294724196172478	30.6	20.6	36.2	9.2	37.0
230-234	26.922566573719838	30.0	20.2	36.0	9.0	37.0
235-239	25.863549032037838	28.4	18.8	34.6	9.0	37.0
240-244	24.833354464329254	26.6	17.0	33.8	8.4	37.0
245-249	24.01361574983265	25.0	16.0	33.0	8.0	36.4
250-254	23.176940024913886	24.2	14.8	32.2	8.0	36.0
255-259	22.591320037060516	23.4	13.8	31.2	8.0	36.0
260-264	21.34794504758907	21.2	12.2	30.2	8.0	35.8
265-269	20.6065751384228	20.6	11.6	28.8	7.6	34.8
270-274	19.670855628011722	19.2	9.4	27.6	7.4	33.8
275-279	18.38175257229239	18.0	8.0	25.8	7.0	32.2
280-284	18.16852338669279	17.6	8.0	25.2	7.0	31.8
285-289	16.98796597170811	15.6	8.0	23.6	7.0	30.2
290-294	16.316045518825057	14.4	8.0	22.4	7.0	29.6
295-299	15.488546261365297	13.0	8.0	21.0	7.0	27.2
300-301	13.534103654184602	11.0	8.0	18.0	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.07784326004767905
1101	2	0.007419100344343121
1101	3	-0.04150595580745886
1101	4	0.05552235096539704
1101	5	0.023376117376038508
1101	6	0.18322854164537006
1101	7	0.16975413232015057
1101	8	0.08441707491472528
1101	9	0.17395068911887535
1101	10-14	0.08948373612564353
1101	15-19	0.03549850880505545
1101	20-24	0.13372331701574325
1101	25-29	0.16231736619954518
1101	30-34	0.1613898206383766
1101	35-39	0.10529420118550092
1101	40-44	0.13702676455640983
1101	45-49	0.18122629621855424
1101	50-54	0.14523875620753302
1101	55-59	0.20922130455829802
1101	60-64	0.3322194842023123
1101	65-69	0.41593618688083467
1101	70-74	0.25000123383885864
1101	75-79	0.2496598142224684
1101	80-84	0.3006452588887072
1101	85-89	0.33642375281132075
1101	90-94	0.4202371734300101
1101	95-99	0.23267976980802985
1101	100-104	0.2586004570740741
1101	105-109	0.35163723578278194
1101	110-114	0.43425164307455333
1101	115-119	0.3050508163535923
1101	120-124	0.25946167206841864
1101	125-129	0.2863932134917988
1101	130-134	0.3427889821297967
1101	135-139	0.44509847200090036
1101	140-144	0.5141695689191152
1101	145-149	0.668536707430242
1101	150-154	0.9097355240358596
1101	155-159	0.6820219108047922
1101	160-164	0.5817552121219478
1101	165-169	0.5722000728660319
1101	170-174	0.6257584081654599
1101	175-179	0.6242441961681209
1101	180-184	0.38359192267611775
1101	185-189	0.3374807660728507
1101	190-194	0.7314632747184149
1101	195-199	0.5191309847651908
1101	200-204	-0.08770266671567839
1101	205-209	0.10411199630558698
1101	210-214	-0.2855209168205164
1101	215-219	0.0033206894938651033
1101	220-224	0.166595087113933
1101	225-229	0.9583968881368676
1101	230-234	0.8141163421138984
1101	235-239	1.0287572859397827
1101	240-244	0.9630255147890168
1101	245-249	0.548379700424352
1101	250-254	0.6806055030803684
1101	255-259	0.8678108123787069
1101	260-264	0.7005720849689432
1101	265-269	0.18512571001814493
1101	270-274	0.2952371601949757
1101	275-279	0.703083961823733
1101	280-284	0.054270075515272964
1101	285-289	0.4385572074282642
1101	290-294	0.23104075983180294
1101	295-299	0.5754911008493249
1101	300-301	0.49155637629857907
1102	1	-0.01793603595817217
1102	2	0.020336937261326682
1102	3	0.05112031612034684
1102	4	0.07802856672118708
1102	5	-0.029886578880692127
1102	6	-0.028127298862941075
1102	7	0.14260624337498484
1102	8	0.059677791338138775
1102	9	0.08835742976070549
1102	10-14	0.015726311750668742
1102	15-19	0.0053831720031780605
1102	20-24	-0.0431839991590266
1102	25-29	-0.01002079393673938
1102	30-34	0.0683434096618285
1102	35-39	0.030637218819833834
1102	40-44	-0.06322416780695761
1102	45-49	0.0038504962277627897
1102	50-54	-0.03460205138028982
1102	55-59	-0.058586835779657065
1102	60-64	0.08077029225056265
1102	65-69	0.16225277604239352
1102	70-74	-0.03639719610726644
1102	75-79	-0.13654984091818534
1102	80-84	0.05444320078289877
1102	85-89	0.025116257070258996
1102	90-94	0.2739257640649342
1102	95-99	0.07470921407341535
1102	100-104	0.027991888539609988
1102	105-109	-0.08668807845819515
1102	110-114	0.11415518375321909
1102	115-119	0.11569644920961508
1102	120-124	0.252194417590772
1102	125-129	0.014120664111075598
1102	130-134	0.12621133122505768
1102	135-139	0.16816813450839163
1102	140-144	0.008873514772965052
1102	145-149	0.3213504879910474
1102	150-154	0.7666447970226429
1102	155-159	0.33434303516595065
1102	160-164	0.2342271261142983
1102	165-169	0.4303778204603006
1102	170-174	0.1928701536417634
1102	175-179	0.5205907130427434
1102	180-184	0.5566932394954947
1102	185-189	0.7310474588303535
1102	190-194	0.9461428179754776
1102	195-199	0.7113451751054427
1102	200-204	0.3006197698605888
1102	205-209	0.2864673073235977
1102	210-214	-0.709402656311287
1102	215-219	-0.05258255190767258
1102	220-224	0.7451242551235104
1102	225-229	1.2916367932890367
1102	230-234	0.7668455193320014
1102	235-239	1.1938368245925695
1102	240-244	0.8923569757218459
1102	245-249	1.2563715508778728
1102	250-254	0.8704352604356806
1102	255-259	0.5920960004628668
1102	260-264	1.5139975419421035
1102	265-269	1.1717992447112664
1102	270-274	0.9759752236417256
1102	275-279	1.483389800029915
1102	280-284	0.6720603743319806
1102	285-289	1.2650524420154916
1102	290-294	1.0574214633632728
1102	295-299	1.085938174839841
1102	300-301	0.5569498681720546
1103	1	0.02054119966670953
1103	2	0.0546762262147098
1103	3	0.04826343239097497
1103	4	0.05649990718917053
1103	5	0.08351583359890213
1103	6	-0.02385331010908942
1103	7	0.0030950879242297447
1103	8	0.0029369061576502986
1103	9	0.07058126433353351
1103	10-14	0.025732206907576938
1103	15-19	0.009305718710955091
1103	20-24	0.09058164568043736
1103	25-29	0.052982561767954905
1103	30-34	0.10308402275813933
1103	35-39	-0.46670996057432745
1103	40-44	-0.3340930232395678
1103	45-49	-0.18973285222135416
1103	50-54	0.0649059297552057
1103	55-59	-0.22939673964324925
1103	60-64	0.2609036134912657
1103	65-69	0.26807537716700836
1103	70-74	0.20009155774725684
1103	75-79	0.31142993415889464
1103	80-84	0.3230061429196738
1103	85-89	0.16446189465415273
1103	90-94	0.24777167373299136
1103	95-99	0.24144919230668904
1103	100-104	0.1891768150768911
1103	105-109	0.005732110186826844
1103	110-114	0.15207918157654632
1103	115-119	0.09965482033242523
1103	120-124	0.3051261244654313
1103	125-129	0.27798108493458074
1103	130-134	0.31435753111622944
1103	135-139	0.4999022175847472
1103	140-144	0.35262011372091706
1103	145-149	0.7018896681107663
1103	150-154	0.8821558617786707
1103	155-159	-0.14845897825686194
1103	160-164	-0.6473822517199181
1103	165-169	0.305747401450688
1103	170-174	0.7668226296076739
1103	175-179	0.47214159278154
1103	180-184	0.5438213911370653
1103	185-189	0.5488644368822193
1103	190-194	0.41797757219278253
1103	195-199	0.1891703156822686
1103	200-204	-0.03945369286388001
1103	205-209	0.6202828342599247
1103	210-214	0.6147334764657941
1103	215-219	0.3296118068806493
1103	220-224	0.7105559619981712
1103	225-229	1.248364074261282
1103	230-234	0.40295562272398655
1103	235-239	0.5760538570612788
1103	240-244	0.7447966637319965
1103	245-249	0.7632053981479601
1103	250-254	0.8524890424055691
1103	255-259	-0.2013195627513511
1103	260-264	1.0615555865639053
1103	265-269	0.045191680771491605
1103	270-274	0.22261044947158481
1103	275-279	1.2261274202004202
1103	280-284	0.09433273058084524
1103	285-289	0.32841177450234227
1103	290-294	0.5172006217162526
1103	295-299	0.7741025136753219
1103	300-301	-0.9624984811840118
2116	1	-0.056801393853383786
2116	2	0.08216462315618145
2116	3	0.05247614956573443
2116	4	0.04065630640116069
2116	5	0.059029762834157395
2116	6	-0.23168440074093866
2116	7	-0.1354348574273061
2116	8	0.03853631403994484
2116	9	0.14617644280360054
2116	10-14	0.087284623794055
2116	15-19	0.1308044888424078
2116	20-24	-0.08772480862521803
2116	25-29	-0.20179110902795117
2116	30-34	0.13076441275445205
2116	35-39	-0.14523678951686492
2116	40-44	-0.20850452278880738
2116	45-49	-0.047531109258315496
2116	50-54	0.16273390397805088
2116	55-59	0.08965954492941108
2116	60-64	0.28713598630281467
2116	65-69	0.2879463800942048
2116	70-74	-0.9068697208708443
2116	75-79	0.06724593246713795
2116	80-84	-0.1462621687657517
2116	85-89	0.003122061382036634
2116	90-94	0.1769663046054788
2116	95-99	0.16166912174228543
2116	100-104	0.09836020461687411
2116	105-109	0.16576082566981398
2116	110-114	0.3004029987323733
2116	115-119	0.18420021416860521
2116	120-124	-0.22963797450282186
2116	125-129	-0.054424560217732676
2116	130-134	0.048386491459922354
2116	135-139	0.1904105261537623
2116	140-144	0.30224113612566583
2116	145-149	0.3651377453977034
2116	150-154	0.9088879730344743
2116	155-159	-0.42206243747086347
2116	160-164	-0.747460718446689
2116	165-169	0.4425349402666754
2116	170-174	0.8078089730582008
2116	175-179	0.6796983281205087
2116	180-184	0.7077762254665387
2116	185-189	1.1759098585434984
2116	190-194	1.009222688891164
2116	195-199	0.6601103581989847
2116	200-204	0.46002208262110855
2116	205-209	0.4990272553312991
2116	210-214	0.5897003646201
2116	215-219	0.880031965594906
2116	220-224	1.386239624223954
2116	225-229	1.010751243560204
2116	230-234	1.2272675084853333
2116	235-239	0.9638264037239423
2116	240-244	1.7780927114575782
2116	245-249	1.0369696863267563
2116	250-254	1.0017555732858412
2116	255-259	0.5992964845290238
2116	260-264	0.06274983901529296
2116	265-269	0.6450831629578744
2116	270-274	0.9039771061212214
2116	275-279	1.113318422682422
2116	280-284	-0.01906003712425175
2116	285-289	-1.3175413458021783
2116	290-294	0.2355774178251977
2116	295-299	0.5084113943279878
2116	300-301	1.0756051839355774
1117	1	-0.11325930039908627
1117	2	0.0029040632108063846
1117	3	-0.05592790857493668
1117	4	0.02548705462084655
1117	5	-0.07573757693756988
1117	6	-0.37332911832946536
1117	7	-0.18540559198979167
1117	8	-0.11530233584787197
1117	9	-0.02434350646964134
1117	10-14	0.09810795810290784
1117	15-19	0.011738056299243738
1117	20-24	-0.041841480169459544
1117	25-29	0.12061646096521628
1117	30-34	0.055479074738663314
1117	35-39	-0.22866767430858914
1117	40-44	-0.1311803592925571
1117	45-49	0.0720230805768125
1117	50-54	0.14306752996576222
1117	55-59	0.10152180227015606
1117	60-64	0.12717110482784477
1117	65-69	0.1847223043942705
1117	70-74	-0.11068398289405224
1117	75-79	0.1506719396859424
1117	80-84	-0.034580381623037226
1117	85-89	0.059838479292487534
1117	90-94	0.25605127192574173
1117	95-99	0.18726662442495012
1117	100-104	0.25271587723418776
1117	105-109	0.18751480089174066
1117	110-114	0.22038641498445344
1117	115-119	0.32549374650691476
1117	120-124	0.34359390531378153
1117	125-129	0.3146174844608396
1117	130-134	0.16130230455132022
1117	135-139	0.26713474818087946
1117	140-144	0.27646316324497633
1117	145-149	0.6530608748484852
1117	150-154	0.8993620731222691
1117	155-159	-0.19237265110856328
1117	160-164	-0.7922368378496714
1117	165-169	0.2692689174396321
1117	170-174	0.4565603143907495
1117	175-179	0.7095149313787275
1117	180-184	0.7783303177502177
1117	185-189	0.9910059467300201
1117	190-194	1.1580179283799978
1117	195-199	0.7723238007899553
1117	200-204	0.8855561768558218
1117	205-209	1.1266148077652076
1117	210-214	0.9623713235509328
1117	215-219	0.36365795330342365
1117	220-224	1.2392198212779029
1117	225-229	0.7057663640362541
1117	230-234	1.1371202057831624
1117	235-239	0.8170504794238695
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2108	190-194	-0.7036141171775014
2108	195-199	-1.608385446605375
2108	200-204	-1.1244515886266129
2108	205-209	-0.5629729745424292
2108	210-214	-0.005528113451592986
2108	215-219	-1.1315306832072913
2108	220-224	-0.4987857439845058
2108	225-229	-0.1551732593378432
2108	230-234	-0.2638051808731525
2108	235-239	-0.7814028953253711
2108	240-244	-0.5226773655501233
2108	245-249	-0.25871853921221444
2108	250-254	-0.5903499059187922
2108	255-259	-0.7332585620204064
2108	260-264	0.12605378134982104
2108	265-269	-0.9425627658141096
2108	270-274	-0.7455081409109461
2108	275-279	-0.4064605701422366
2108	280-284	-0.5167842871611974
2108	285-289	-0.2731198887310988
2108	290-294	-0.8412595195998911
2108	295-299	-0.044076988838687825
2108	300-301	-0.17707530799421
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	44.0
14	154.0
15	266.0
16	295.0
17	395.0
18	660.0
19	618.0
20	569.0
21	751.0
22	1043.0
23	1682.0
24	2402.0
25	3431.0
26	4760.0
27	6505.0
28	8993.0
29	12454.0
30	17116.0
31	25141.0
32	34877.0
33	43822.0
34	40122.0
35	17767.0
36	1700.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.411584514378685	24.361526219428402	15.375237723704101	31.851651542488817
2	41.98389020157197	24.338917531485922	18.806438599679932	14.870753667262177
3	41.095945065321374	22.901714270515168	20.30659154080425	15.695749123359207
4	41.41158008130261	22.761185759186443	20.105329887355534	15.721904272155406
5	27.736870336958113	22.66144154767552	34.20605824175337	15.395629873613
6	26.586487097532107	22.567903642658603	34.63783985069399	16.20776940911529
7	17.727427884935075	45.71565363490072	20.780044064776106	15.776874415388093
8	26.02924943589107	21.625874978388754	35.44510300252242	16.899772583197755
9	16.249440324146523	20.14035118828604	37.072485226774006	26.537723260793435
10-14	25.730282785922327	33.87730132061336	23.816878493818074	16.57553739964624
15-19	21.514427446060548	34.67605296639285	22.29908190994649	21.510437677600112
20-24	24.715817658715206	30.918932337959987	26.47699011867345	17.88825988465136
25-29	19.59056109443782	26.101685898828336	36.46807963577847	17.83967337095537
30-34	19.487359083594516	28.529061030158214	30.99243273915337	20.991147147093898
35-39	20.727467782619684	29.932572913018614	27.70885329621371	21.631106008147995
40-44	20.133701574185313	29.13169339072689	28.80666025348329	21.92794478160451
45-49	21.17910957234115	30.072569455219284	26.651653315719244	22.096667656720324
50-54	21.890352296555058	29.540954973246386	27.6299445422184	20.938748187980156
55-59	21.569397589293235	27.151970280658045	29.7529446707776	21.525687459271115
60-64	21.753192923037368	26.302238260106304	28.997016539806808	22.94755227704952
65-69	20.3639555451132	27.27077671925773	28.991430863962194	23.37383687166688
70-74	20.97802524193513	26.387087336031602	29.31823723163266	23.31665019040062
75-79	20.308630755795136	26.255868284443896	30.64496823700998	22.79053272275099
80-84	23.02406716996857	25.95583769621903	27.570452661397216	23.44964247241518
85-89	20.86285392571051	28.383744796676968	26.515469218936328	24.237932058676197
90-94	20.04113894590317	29.885759629749487	29.033367763557454	21.039733660789885
95-99	22.04710586628956	30.057496996590967	26.74235405205318	21.153043085066297
100-104	22.333039272620876	28.42958280321132	26.941842475075028	22.29553544909277
105-109	23.2020995048254	28.086906023220454	24.430504883033286	24.280489588920855
110-114	21.56434388257668	28.278592232364115	28.9792842355382	21.177779649521007
115-119	23.250774680042735	27.989201026700417	27.112693226703076	21.64733106655377
120-124	19.073487101965185	28.64937471462073	28.129729538029142	24.147408645384946
125-129	20.700692003174083	25.219326438422357	30.726359513602898	23.353622044800666
130-134	24.596479250987468	28.796109532443463	26.106296297938176	20.50111491863089
135-139	23.688762595477375	27.80380978557211	25.234842204657387	23.272585414293122
140-144	20.780753356946853	29.550175771466066	27.045665116567736	22.62340575501935
145-149	20.83689457426473	27.487046540247047	29.06477971138876	22.611279174099465
150-154	23.06103902436862	26.07553075003214	25.94714886712741	24.91628135847183
155-159	21.44722201288252	27.142394836352995	29.819352150263544	21.591031000500937
160-164	24.476431551088986	29.308041156678204	26.25640025357195	19.959127038660856
165-169	20.25773904254423	26.393382304046952	28.451836845068424	24.89704180834039
170-174	21.897622541305186	29.421616565518647	26.289116354947534	22.39164453822863
175-179	20.065786848836538	29.227270510734694	28.706650057408332	22.000292583020432
180-184	19.829415232935983	29.976017058476707	26.826405174286382	23.368162534300925
185-189	21.805059913023047	28.02998532651822	27.782353697407093	22.38260106305164
190-194	19.913200370605157	28.23337485647916	27.387809927430546	24.465614845485135
195-199	20.76115916073004	30.33314566644649	27.465832066212425	21.439863106611046
200-204	21.755764107156313	28.020941851341224	27.744317904750925	22.47897613675153
205-209	20.922345806531695	27.96171595508407	28.428784849519236	22.687153388865
210-214	22.77129317261955	27.52248677835063	28.012607668334983	21.69361238069484
215-219	22.34740243907845	27.633579664593466	28.184345035176463	21.834672861151624
220-224	19.048661875989133	27.52771780810987	28.596621109421616	24.826999206479382
225-229	21.11944036847728	29.207144345389825	26.83997038705187	22.833444899081023
230-234	20.665848025286266	27.830762888060395	28.422401219982536	23.080987866670803
235-239	21.53056384294498	27.614074129897997	26.40925271636736	24.446109310789662
240-244	22.481635982391822	31.57671216480404	23.863159807959143	22.078492044844996
245-249	20.37175775899139	29.804634337720604	25.200352872854946	24.623255030433068
250-254	20.970134366535596	27.480195232669995	26.430620143011037	25.11905025778337
255-259	21.16456908284089	28.33205512973397	27.398892617598424	23.10448316982671
260-264	20.791658724072047	29.36318862295358	27.065347974305894	22.77980467866848
265-269	20.2078226060281	28.782721642720666	27.19727631806434	23.812179433186895
270-274	18.75581287099305	30.985251155924587	25.63701086546944	24.62192510761292
275-279	20.264034010559588	29.377285804847126	24.562787872877113	25.79589231171618
280-284	17.57572802191713	30.642042406805658	27.297552498703325	24.484677072573888
285-289	20.295154204550997	28.103219743147573	28.335778913630378	23.265847138671052
290-294	19.59809732375198	31.007505197781686	26.104257082947285	23.290140395519046
295-299	19.127038660856382	31.74853819316686	23.682378965940675	25.442044180036085
300-301	15.064257437593373	34.52102829632454	28.038541163327825	22.376173102754272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.25
21	0.25
22	0.0
23	0.0
24	0.0
25	0.25
26	0.0
27	0.0
28	0.25
29	0.25
30	0.75
31	2.25
32	3.5
33	9.0
34	22.0
35	55.25
36	148.75
37	496.0
38	1615.0
39	2588.25
40	4248.5
41	5539.0
42	7679.75
43	10489.75
44	11017.75
45	8401.75
46	6086.75
47	4964.5
48	4296.5
49	3155.75
50	2391.75
51	1513.75
52	361.0
53	70.75
54	33.25
55	17.0
56	12.75
57	10.5
58	9.25
59	7.25
60	5.5
61	5.0
62	2.25
63	1.75
64	0.75
65	0.75
66	0.5
67	0.5
68	0.5
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	8.866152134304473E-5
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	225577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.97209719474322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.62426692255343	6.215906178957961
2	11.785191169942586	4.000388199897902
3	9.358578613941459	4.765041175213793
4	8.033687836033392	5.453941231415404
5	6.6252078193050625	5.622183552230915
6	5.603242965907859	5.705927053388968
7	4.186794650979545	4.974108002559911
8	3.2398737556760073	4.398996182402475
9	2.7540784011246036	4.206823767524681
>10	11.612903840394665	30.670086861451
>50	0.09473304766487976	0.9935379640614566
>100	0.023649013410225864	0.942641844292773
>500	0.01838835188474311	2.359837890798031
>1k	0.034149796357379816	13.287407643954918
>5k	0.00525381482421228	6.403172451849833
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8484	3.7610217353719575	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	5878	2.6057621122720844	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4647	2.060050448405644	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	3718	1.6482176817672014	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3168	1.4043984980738284	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	2735	1.2124463043661367	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2519	1.1166918613156485	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2331	1.0333500312531863	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2061	0.9136569774400759	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	2041	0.9047908253057714	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1749	0.7753450041449261	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1357	0.6015684223125585	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1351	0.5989085766722672	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	1104	0.4894115978136069	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1022	0.45306037406295857	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	986	0.43710130022121046	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	909	0.4029666145041383	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	905	0.4011933840772774	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	739	0.32760432136255024	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	719	0.31873816922824577	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	519	0.23007664788520107	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	516	0.2287467250650554	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	483	0.214117574043453	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	338	0.14983797106974558	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	334	0.1480647406428847	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	231	0.10240405715121666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	8.866152134304472E-4	0.0	0.0
15-19	0.0015072458628317604	0.0	0.0
20-24	0.0026598456402913417	0.0	0.0
25-29	0.0033691378110356995	0.0	0.0
30-34	0.005231029759239639	0.0	0.0
35-39	0.0071815832287866225	0.0	0.0
40-44	0.008954813655647518	0.0	0.0
45-49	0.010639382561165367	0.0	0.0
50-54	0.013831197329514978	0.0	0.0
55-59	0.0184415964393533	0.0	0.0
60-64	0.025091210540081656	0.0	0.0
65-69	0.03652854679333443	0.0	0.0
70-74	0.05399486649791424	0.0	0.0
75-79	0.07580560074830325	0.0	0.0
80-84	0.11038359407209068	0.0	0.0
85-89	0.15329577040212436	0.0	0.0
90-94	0.21003914406167296	0.0	0.0
95-99	0.2792837922305909	0.0	0.0
100-104	0.36413286815588464	0.0	0.0
105-109	0.47850623068841236	0.0	0.0
110-114	0.6133604046511835	0.0	0.0
115-119	0.7600952224739226	0.0	0.0
120-124	0.9350244040837496	0.0	0.0
125-129	1.1186424147851954	0.0	0.0
130-134	1.3275289590694086	0.0	0.0
135-139	1.5666490821316001	0.0	0.0
140-144	1.845932874362191	0.0	0.0
145-149	2.1597946599165696	0.0	0.0
150-154	2.4921867034316443	0.0	0.0
155-159	2.8801695208288076	0.0	0.0
160-164	3.3326092642423655	0.0	0.0
165-169	3.7808819161527993	0.0	0.0
170-174	4.290419679311277	0.0	0.0
175-179	4.847214033345598	0.0	0.0
180-184	5.450821670649047	0.0	0.0
185-189	6.146459967106575	0.0	0.0
190-194	6.923312217114334	0.0	0.0
195-199	7.674363964411265	0.0	0.0
200-204	8.47675073256582	0.0	0.0
205-209	9.316020693599082	0.0	0.0
210-214	10.201660630294755	0.0	0.0
215-219	11.1749868115987	0.0	0.0
220-224	12.17171963453721	0.0	0.0
225-229	13.256050040562645	0.0	0.0
230-234	14.35022187545716	0.0	0.0
235-239	15.474893273693684	0.0	0.0
240-244	16.623769267256854	0.0	0.0
245-249	17.762626508908266	0.0	0.0
250-254	18.98287502715259	0.0	0.0
255-259	20.15852680016136	0.0	0.0
260-264	21.360245060444992	0.0	0.0
265-269	22.491388749739556	0.0	0.0
270-274	23.56685300363069	0.0	0.0
275-279	24.590716252100172	0.0	0.0
280-284	25.563953771882773	0.0	0.0
285-289	26.490466669917584	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	3055	0.0	293.06876	1
GGGTTAT	3060	0.0	292.58987	2
GTTATTA	3100	0.0	288.33868	4
GGGGGGA	2200	0.0	286.2841	1
GGTTATT	3135	0.0	285.59012	3
TTATTAG	3210	0.0	278.45795	5
TTAGTAG	3230	0.0	278.1037	8
TAGTAGA	3300	0.0	272.20453	9
GGGGAGC	2340	0.0	268.52563	3
TATTAGT	3360	0.0	266.9048	6
GGGAGCG	1505	0.0	264.61792	4
GGAGCGA	1515	0.0	262.87128	5
ATTAGTA	3420	0.0	262.22223	7
CGAAAGC	1610	0.0	247.36024	9
GCGAAAG	1645	0.0	242.09726	8
GAGCGAA	1660	0.0	239.90962	6
AGCGAAA	1675	0.0	237.7612	7
GGGGGAG	2845	0.0	221.37961	2
GGGAGCA	1070	0.0	213.66821	4
AGACCCC	85	0.0	208.23529	295
>>END_MODULE